首页 > 最新文献

Journal of Reproduction and Development最新文献

英文 中文
Continuous acceleration of neural activity of the GnRH pulse generator during chronic peripheral infusion of neurokinin 3 receptor agonist in goats. 慢性外周输注神经激肽3受体激动剂对山羊GnRH脉冲发生器神经活动持续加速的影响。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-025
Takashi Yamamura, Hiroaki Okamura, Yoshihiro Wakabayashi

Secretion of pulsatile gonadotropin-releasing hormone (GnRH) is essential for reproduction. Kisspeptin neurons in the arcuate nucleus (ARC), which coexpress neurokinin B (NKB) and its receptor (NK3R), are believed to be components of the GnRH pulse generator that regulates pulsatile GnRH secretion. We examined the effects of peripheral infusion of senktide, an NK3R selective agonist, on GnRH pulse generator activity by monitoring multiple unit activity (MUA) in the goat ARC. Previous studies have shown that characteristic increases in MUA (MUA volleys) reflect GnRH pulse generator activity. Senktide was infused intravenously or intravaginally for 2 h while recording MUA. Both infusions significantly increased the MUA volley frequency compared with the control. These results demonstrate that peripherally administered senktide acts centrally to sustainably accelerate the neural activity of the GnRH pulse generator throughout the infusion period. This suggests the possibility of practical applications of NK3R agonists for improving reproductive activity in farm animals.

脉动性促性腺激素释放激素(GnRH)的分泌是生殖所必需的。弓形核(ARC)中的Kisspeptin神经元共表达神经激肽B (NKB)及其受体(NK3R),被认为是GnRH脉冲发生器的组成部分,调节脉动性GnRH分泌。我们通过监测山羊ARC中的多单位活性(MUA),研究了外周输注senktide(一种NK3R选择性激动剂)对GnRH脉冲发生器活性的影响。先前的研究表明,MUA (MUA截击)的特征性增加反映了GnRH脉冲发生器的活性。Senktide静脉或静脉滴注2小时,同时记录MUA。与对照组相比,两组注射均显著增加了MUA的射频。这些结果表明,外周给药senktide在整个输注期间持续加速GnRH脉冲发生器的神经活动。这表明NK3R激动剂在提高农场动物生殖活性方面具有实际应用的可能性。
{"title":"Continuous acceleration of neural activity of the GnRH pulse generator during chronic peripheral infusion of neurokinin 3 receptor agonist in goats.","authors":"Takashi Yamamura,&nbsp;Hiroaki Okamura,&nbsp;Yoshihiro Wakabayashi","doi":"10.1262/jrd.2023-025","DOIUrl":"https://doi.org/10.1262/jrd.2023-025","url":null,"abstract":"<p><p>Secretion of pulsatile gonadotropin-releasing hormone (GnRH) is essential for reproduction. Kisspeptin neurons in the arcuate nucleus (ARC), which coexpress neurokinin B (NKB) and its receptor (NK3R), are believed to be components of the GnRH pulse generator that regulates pulsatile GnRH secretion. We examined the effects of peripheral infusion of senktide, an NK3R selective agonist, on GnRH pulse generator activity by monitoring multiple unit activity (MUA) in the goat ARC. Previous studies have shown that characteristic increases in MUA (MUA volleys) reflect GnRH pulse generator activity. Senktide was infused intravenously or intravaginally for 2 h while recording MUA. Both infusions significantly increased the MUA volley frequency compared with the control. These results demonstrate that peripherally administered senktide acts centrally to sustainably accelerate the neural activity of the GnRH pulse generator throughout the infusion period. This suggests the possibility of practical applications of NK3R agonists for improving reproductive activity in farm animals.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"218-222"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/f5/jrd-69-218.PMC10435531.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10400331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progesterone modulates HSD11B1-mediated cortisol production in luteinized bovine granulosa cells. 黄体酮调节hsd11b1介导的黄体化牛颗粒细胞中皮质醇的产生。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-005
Memory Mukangwa, Masafumi Tetsuka

Progesterone (P4) and cortisol production increase in luteinized granulosa cells (LGCs) during the periovulatory period, but their interaction is not well established. Therefore, we investigated their interaction in cultured bovine LGCs. Granulosa cells were collected from follicles of 2-5 mm in diameter and cultured in DMEM/F-12 supplemented with 10% fetal calf serum for up to 14 days. P4 production and the expression of steroidogenic acute regulatory protein (STAR), cholesterol side-chain cleavage enzyme (CYP11A1), and 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) rapidly increased until day 10 and remained high thereafter. No de novo production of cortisol from P4 was detected during the culture period. The expression of 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1), which converts cortisone to cortisol, increased dramatically on day two, decreased until day 8, and remained relatively constant. To investigate how P4 and cortisol influence each other's production, LGCs were treated with trilostane (a P4 synthesis inhibitor), nomegestrol acetate (NA, a synthetic progestogen), P4, and/or cortisol for 24 h on days 6 and 12 of culture. Trilostane suppressed P4 and STAR expression while elevating HSD11B1 and HSD3B1 expression and cortisol production. Concomitant treatment with NA or P4 dose-dependently decreased cortisol production and HSD11B1 and HSD3B1 expression but elevated STAR expression in both days 6 and 12. Conversely, cortisol treatment increased HSD11B1 and HSD3B1 expression and decreased STAR expression without influencing P4 production. These results indicate that progestogens suppress cortisol production by modulating HSD11B1 expression and that progestogens and cortisol differentially regulate STAR, HSD3B1, and HSD11B1 expression in bovine LGCs.

在排卵期黄体化颗粒细胞(lgc)中,黄体酮(P4)和皮质醇的产生增加,但它们之间的相互作用尚未得到很好的证实。因此,我们研究了它们在培养的牛LGCs中的相互作用。从直径为2-5 mm的卵泡中收集颗粒细胞,在添加10%胎牛血清的DMEM/F-12中培养14天。P4的产生和类固醇急性调节蛋白(STAR)、胆固醇侧链切割酶(CYP11A1)、3β-羟基类固醇脱氢酶1型(HSD3B1)的表达迅速增加,直到第10天,此后一直保持较高水平。在培养期间,未检测到P4重新产生皮质醇。将可的松转化为皮质醇的11β-羟基类固醇脱氢酶1型(HSD11B1)的表达在第2天急剧增加,直到第8天下降,并保持相对稳定。为了研究P4和皮质醇如何相互影响产生,在培养的第6天和第12天,用三叶甾烷(一种P4合成抑制剂)、醋酸异孕酮(NA,一种合成孕激素)、P4和/或皮质醇处理LGCs 24小时。Trilostane抑制P4和STAR的表达,同时提高HSD11B1和HSD3B1的表达和皮质醇的产生。在第6天和第12天,NA或P4的剂量依赖性治疗降低了皮质醇的产生和HSD11B1和HSD3B1的表达,但升高了STAR的表达。相反,皮质醇处理增加了HSD11B1和HSD3B1的表达,降低了STAR的表达,但不影响P4的产生。这些结果表明,孕激素通过调节HSD11B1的表达来抑制皮质醇的产生,并且孕激素和皮质醇对牛LGCs中STAR、HSD3B1和HSD11B1的表达有差异。
{"title":"Progesterone modulates HSD11B1-mediated cortisol production in luteinized bovine granulosa cells.","authors":"Memory Mukangwa,&nbsp;Masafumi Tetsuka","doi":"10.1262/jrd.2023-005","DOIUrl":"https://doi.org/10.1262/jrd.2023-005","url":null,"abstract":"<p><p>Progesterone (P4) and cortisol production increase in luteinized granulosa cells (LGCs) during the periovulatory period, but their interaction is not well established. Therefore, we investigated their interaction in cultured bovine LGCs. Granulosa cells were collected from follicles of 2-5 mm in diameter and cultured in DMEM/F-12 supplemented with 10% fetal calf serum for up to 14 days. P4 production and the expression of steroidogenic acute regulatory protein (STAR), cholesterol side-chain cleavage enzyme (CYP11A1), and 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) rapidly increased until day 10 and remained high thereafter. No de novo production of cortisol from P4 was detected during the culture period. The expression of 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1), which converts cortisone to cortisol, increased dramatically on day two, decreased until day 8, and remained relatively constant. To investigate how P4 and cortisol influence each other's production, LGCs were treated with trilostane (a P4 synthesis inhibitor), nomegestrol acetate (NA, a synthetic progestogen), P4, and/or cortisol for 24 h on days 6 and 12 of culture. Trilostane suppressed P4 and STAR expression while elevating HSD11B1 and HSD3B1 expression and cortisol production. Concomitant treatment with NA or P4 dose-dependently decreased cortisol production and HSD11B1 and HSD3B1 expression but elevated STAR expression in both days 6 and 12. Conversely, cortisol treatment increased HSD11B1 and HSD3B1 expression and decreased STAR expression without influencing P4 production. These results indicate that progestogens suppress cortisol production by modulating HSD11B1 expression and that progestogens and cortisol differentially regulate STAR, HSD3B1, and HSD11B1 expression in bovine LGCs.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"206-213"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/1d/jrd-69-206.PMC10435524.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10419215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system. 在体外培养8天的牛卵母细胞中,预成熟培养可促进其发育能力。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-022
Madalitso Chelenga, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.

在这项研究中,我们评估了预成熟培养(预ivm)对牛卵母细胞发育能力的影响,这些卵母细胞来自8天的体外生长(IVG)培养系统。IVG卵母细胞在体外成熟前进行5h的预ivm,然后进行体外受精(IVF)。预ivm组和未预ivm组的卵母细胞进入生发囊泡破裂期的比例相似。尽管体外受精后中期卵母细胞和卵裂率与体外受精前后无明显差异,但体外受精组的囊胚率(22.5%)显著高于未体外受精组(11.0%,P < 0.05)。综上所述,体外培养可提高体外培养8天的牛卵母细胞的发育能力。
{"title":"Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system.","authors":"Madalitso Chelenga,&nbsp;Yojiro Yanagawa,&nbsp;Seiji Katagiri,&nbsp;Masashi Nagano","doi":"10.1262/jrd.2023-022","DOIUrl":"https://doi.org/10.1262/jrd.2023-022","url":null,"abstract":"<p><p>In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"214-217"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/84/jrd-69-214.PMC10435529.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10034465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of calcium-sensing receptor in regulating activation susceptibility of postovulatory aging mouse oocytes. 钙敏感受体在调节排卵后衰老小鼠卵母细胞活化敏感性中的作用。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-026
Rui Yang, Chang-Li Ji, Min Zhang, Jie Zhang, Hong-Jie Yuan, Ming-Jiu Luo, Guang-Zhong Jiao, Jing-He Tan

The mechanisms underlying postovulatory oocyte aging (POA) remain largely unknown. The expression of the calcium-sensing receptor (CaSR) in mouse oocytes and its role in POA need to be explored. Our objective was to observe CaSR expression and its role in the susceptibility to activating stimuli (STAS) in POA mouse oocytes. The results showed that, although none of the newly ovulated oocytes were activated, 40% and 94% of the oocytes recovered 19 and 25 h after human chorionic gonadotropin (hCG) injection were activated, respectively, after ethanol treatment. The level of the CaSR functional dimer protein in oocytes increased significantly from 13 to 25 h post hCG. Thus, the CaSR functional dimer level was positively correlated with the STAS of POA oocytes. Aging in vitro with a CaSR antagonist suppressed the elevation of STAS, and cytoplasmic calcium in oocytes recovered 19 h post hCG, whereas aging with a CaSR agonist increased STAS, and cytoplasmic calcium of oocytes recovered 13 h post hCG. Furthermore, the CaSR was more important than the Na-Ca2+ exchanger in regulating oocyte STAS, and T- and L-type calcium channels were inactive in aging oocytes. We conclude that the CaSR is involved in regulating STAS in POA mouse oocytes, and that it is more important than the other calcium channels tested in this connection.

排卵后卵母细胞老化(POA)的机制在很大程度上仍然未知。钙敏感受体(CaSR)在小鼠卵母细胞中的表达及其在POA中的作用有待进一步探讨。我们的目的是观察CaSR表达及其在POA小鼠卵母细胞活化刺激敏感性(STAS)中的作用。结果表明,虽然新排卵的卵母细胞没有被激活,但在人绒毛膜促性腺激素(hCG)注射后19和25 h,经乙醇处理后,分别有40%和94%的卵母细胞被激活。卵母细胞中CaSR功能二聚体蛋白水平在hCG后13 ~ 25 h显著升高。因此,CaSR功能二聚体水平与POA卵母细胞STAS呈正相关。体外衰老加CaSR拮抗剂抑制STAS升高,hCG后19 h卵母细胞胞质钙恢复,而加CaSR激动剂衰老加STAS升高,hCG后13 h卵母细胞胞质钙恢复。此外,在调节卵母细胞STAS方面,CaSR比Na-Ca2+交换器更重要,T型和l型钙通道在衰老的卵母细胞中失活。我们得出结论,CaSR参与了POA小鼠卵母细胞STAS的调节,并且在这方面它比其他钙通道更重要。
{"title":"Role of calcium-sensing receptor in regulating activation susceptibility of postovulatory aging mouse oocytes.","authors":"Rui Yang,&nbsp;Chang-Li Ji,&nbsp;Min Zhang,&nbsp;Jie Zhang,&nbsp;Hong-Jie Yuan,&nbsp;Ming-Jiu Luo,&nbsp;Guang-Zhong Jiao,&nbsp;Jing-He Tan","doi":"10.1262/jrd.2023-026","DOIUrl":"https://doi.org/10.1262/jrd.2023-026","url":null,"abstract":"<p><p>The mechanisms underlying postovulatory oocyte aging (POA) remain largely unknown. The expression of the calcium-sensing receptor (CaSR) in mouse oocytes and its role in POA need to be explored. Our objective was to observe CaSR expression and its role in the susceptibility to activating stimuli (STAS) in POA mouse oocytes. The results showed that, although none of the newly ovulated oocytes were activated, 40% and 94% of the oocytes recovered 19 and 25 h after human chorionic gonadotropin (hCG) injection were activated, respectively, after ethanol treatment. The level of the CaSR functional dimer protein in oocytes increased significantly from 13 to 25 h post hCG. Thus, the CaSR functional dimer level was positively correlated with the STAS of POA oocytes. Aging in vitro with a CaSR antagonist suppressed the elevation of STAS, and cytoplasmic calcium in oocytes recovered 19 h post hCG, whereas aging with a CaSR agonist increased STAS, and cytoplasmic calcium of oocytes recovered 13 h post hCG. Furthermore, the CaSR was more important than the Na-Ca<sup>2+</sup> exchanger in regulating oocyte STAS, and T- and L-type calcium channels were inactive in aging oocytes. We conclude that the CaSR is involved in regulating STAS in POA mouse oocytes, and that it is more important than the other calcium channels tested in this connection.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"185-191"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f0/db/jrd-69-185.PMC10435528.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10418737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of linker histone variant H1a in the regulation of early preimplantation development in mice. 连接蛋白变异H1a参与小鼠早期着床前发育的调控。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2023-013
Satoshi Funaya, Yuan Wang, Masataka G Suzuki, Masahito Ikawa, Fugaku Aoki

Linker histone variants regulate higher-order chromatin structure and various cellular processes. It has been suggested that linker histone variant H1a loosens chromatin structure and activates transcription. However, its role in early mouse development remains to be elucidated. We investigated the functions of H1a during preimplantation development using H1a gene-deleted mice. Although H1a homozygous knockout (KO) mice were born without any abnormalities, the number of offspring were reduced when the mothers but not fathers were homozygous KO animals. Maternal H1a KO compromised development during the morula and blastocyst stages, but not differentiation of the inner cell mass or trophectoderm. Thus, maternal linker histone H1a is important in early development.

连接体组蛋白变异调节高阶染色质结构和各种细胞过程。有研究表明,连接体组蛋白变体H1a使染色质结构松动并激活转录。然而,它在小鼠早期发育中的作用仍有待阐明。我们利用H1a基因缺失的小鼠研究了H1a在着床前发育中的功能。虽然H1a纯合子敲除(KO)小鼠出生时没有任何异常,但当母亲而不是父亲是纯合子KO动物时,后代数量减少。母体H1a KO在桑葚胚和囊胚阶段影响发育,但不影响内细胞群或滋养外胚层的分化。因此,母体连接蛋白H1a在早期发育中很重要。
{"title":"Involvement of linker histone variant H1a in the regulation of early preimplantation development in mice.","authors":"Satoshi Funaya,&nbsp;Yuan Wang,&nbsp;Masataka G Suzuki,&nbsp;Masahito Ikawa,&nbsp;Fugaku Aoki","doi":"10.1262/jrd.2023-013","DOIUrl":"https://doi.org/10.1262/jrd.2023-013","url":null,"abstract":"<p><p>Linker histone variants regulate higher-order chromatin structure and various cellular processes. It has been suggested that linker histone variant H1a loosens chromatin structure and activates transcription. However, its role in early mouse development remains to be elucidated. We investigated the functions of H1a during preimplantation development using H1a gene-deleted mice. Although H1a homozygous knockout (KO) mice were born without any abnormalities, the number of offspring were reduced when the mothers but not fathers were homozygous KO animals. Maternal H1a KO compromised development during the morula and blastocyst stages, but not differentiation of the inner cell mass or trophectoderm. Thus, maternal linker histone H1a is important in early development.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"178-182"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/42/jrd-69-178.PMC10267588.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell cycle regulation for meiosis in mammalian germ cells. 哺乳动物生殖细胞减数分裂的细胞周期调控。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2023-010
Ryuki Shimada, Kei-Ichiro Ishiguro

In mouse fetal gonads, germ cell development is accompanied by changes in cell cycle mode in response to external signals and intrinsic mechanisms of cells. During fetal development, male germ cells undergo G0/G1 arrest, while female germ cells exit the mitotic cell cycle and enter meiosis. In fetal testes, NANOS2 and CYP26B1 force germ cells to stay in G0/G1 arrest phase, preventing them from entering the meiotic cell cycle. In the fetal ovary, external signals, such as RA, BMP, and WNT, promote the competency of female germ cells to enter the meiotic cell cycle. MEIOSIN and STRA8 ensure the establishment of the meiotic cell cycle by activating meiotic genes, such that meiotic entry coincides with the S phase. This review discusses germ cell development from the viewpoint of cell cycle regulation and highlights the mechanism of the entry of germ cells into meiosis.

在小鼠胎儿性腺中,生殖细胞的发育伴随着细胞周期模式的改变,以响应外部信号和细胞的内在机制。在胎儿发育过程中,男性生殖细胞经历G0/G1阻滞,而女性生殖细胞退出有丝分裂周期进入减数分裂。在胎儿睾丸中,NANOS2和CYP26B1迫使生殖细胞停留在G0/G1停滞期,阻止它们进入减数分裂细胞周期。在胎儿卵巢中,RA、BMP、WNT等外部信号促进雌性生殖细胞进入减数分裂细胞周期。MEIOSIN和STRA8通过激活减数分裂基因来确保减数分裂细胞周期的建立,使减数分裂进入与S期一致。本文从细胞周期调控的角度对生殖细胞的发育进行了综述,重点阐述了生殖细胞进入减数分裂的机制。
{"title":"Cell cycle regulation for meiosis in mammalian germ cells.","authors":"Ryuki Shimada,&nbsp;Kei-Ichiro Ishiguro","doi":"10.1262/jrd.2023-010","DOIUrl":"https://doi.org/10.1262/jrd.2023-010","url":null,"abstract":"<p><p>In mouse fetal gonads, germ cell development is accompanied by changes in cell cycle mode in response to external signals and intrinsic mechanisms of cells. During fetal development, male germ cells undergo G0/G1 arrest, while female germ cells exit the mitotic cell cycle and enter meiosis. In fetal testes, NANOS2 and CYP26B1 force germ cells to stay in G0/G1 arrest phase, preventing them from entering the meiotic cell cycle. In the fetal ovary, external signals, such as RA, BMP, and WNT, promote the competency of female germ cells to enter the meiotic cell cycle. MEIOSIN and STRA8 ensure the establishment of the meiotic cell cycle by activating meiotic genes, such that meiotic entry coincides with the S phase. This review discusses germ cell development from the viewpoint of cell cycle regulation and highlights the mechanism of the entry of germ cells into meiosis.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"139-146"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/f7/jrd-69-139.PMC10267585.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9633467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling. MiR-145通过靶向Arpc5和随后的细胞骨架重塑来调节小鼠原代颗粒细胞中的甾体生成。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2022-137
Lanfang Ma, Shuo Wang, Jun Yang, Weicheng Tang, Zhangying Wu, Lili Cao, Aiyue Luo, Fangfang Fu, Shuhong Yang, Shixuan Wang

MicroRNA (miR)-145 is enriched in the follicular granulosa cells (GCs) of 3-week-old mice. Downregulating miR-145 inhibits the proliferation and differentiation of GCs and induces evident changes in their cytoskeleton. In this study, we examined how miR-145 induces cytoskeletal changes in mouse GCs and its potential mechanism in regulating GC steroidogenesis. We found that actin related protein 2/3 complex subunit 5 (Arpc5) is a target of miR-145. The miR-145 antagomir increased ARPC5 expression but not β-ACTIN, β-TUBULIN, and PAXILLIN expression. Arpc5 overexpression inhibited GC proliferation, differentiation, and progesterone synthesis. Furthermore, the expression of progesterone synthesis-associated enzymes was downregulated in the Arpc5 overexpression group, and the GC cytoskeleton exhibited evident changes. We conclude that Arpc5, a new target of miR-145, regulates primary GC proliferation and progesterone production by regulating the cytoskeleton remodeling.

MicroRNA (miR)-145在3周龄小鼠滤泡颗粒细胞(GCs)中富集。下调miR-145抑制GCs的增殖和分化,诱导其细胞骨架发生明显变化。在这项研究中,我们研究了miR-145如何诱导小鼠GC细胞骨架变化及其调节GC类固醇生成的潜在机制。我们发现肌动蛋白相关蛋白2/3复合物亚基5 (Arpc5)是miR-145的靶标。miR-145拮抗剂增加了ARPC5的表达,但没有增加β-ACTIN、β-TUBULIN和PAXILLIN的表达。Arpc5过表达抑制GC增殖、分化和黄体酮合成。Arpc5过表达组黄体酮合成相关酶表达下调,GC细胞骨架发生明显变化。我们得出结论,miR-145的新靶点Arpc5通过调节细胞骨架重塑来调节原代GC增殖和孕酮的产生。
{"title":"MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling.","authors":"Lanfang Ma,&nbsp;Shuo Wang,&nbsp;Jun Yang,&nbsp;Weicheng Tang,&nbsp;Zhangying Wu,&nbsp;Lili Cao,&nbsp;Aiyue Luo,&nbsp;Fangfang Fu,&nbsp;Shuhong Yang,&nbsp;Shixuan Wang","doi":"10.1262/jrd.2022-137","DOIUrl":"https://doi.org/10.1262/jrd.2022-137","url":null,"abstract":"<p><p>MicroRNA (miR)-145 is enriched in the follicular granulosa cells (GCs) of 3-week-old mice. Downregulating miR-145 inhibits the proliferation and differentiation of GCs and induces evident changes in their cytoskeleton. In this study, we examined how miR-145 induces cytoskeletal changes in mouse GCs and its potential mechanism in regulating GC steroidogenesis. We found that actin related protein 2/3 complex subunit 5 (Arpc5) is a target of miR-145. The miR-145 antagomir increased ARPC5 expression but not β-ACTIN, β-TUBULIN, and PAXILLIN expression. Arpc5 overexpression inhibited GC proliferation, differentiation, and progesterone synthesis. Furthermore, the expression of progesterone synthesis-associated enzymes was downregulated in the Arpc5 overexpression group, and the GC cytoskeleton exhibited evident changes. We conclude that Arpc5, a new target of miR-145, regulates primary GC proliferation and progesterone production by regulating the cytoskeleton remodeling.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"154-162"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/8e/jrd-69-154.PMC10267586.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years. 小鼠体细胞核移植:25年来发生了什么变化?
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2022-105
Kimiko Inoue

Somatic cell nuclear transfer (SCNT) is the only reproductive technology used to produce individuals from somatic cells by transferring them to enucleated oocytes. Although more than 25 years have passed since the first mammalian SCNT was reported in sheep, problems such as low birth rates and morphological abnormalities have persisted and limited its practical applications. The mouse is the ideal laboratory animal to unveil these questions due to its established reproductive technologies and extensive knowledge base of its genome and various strains. We investigated the causes of incomplete reprogramming after nuclear transfer of donor somatic cells and found that the loss of imprint in some placenta-specific imprinted genes could induce non-random SCNT abnormalities. By ameliorating aberrantly expressed imprinted genes, we succeeded in increasing the low birth rate and improving morphological abnormalities observed in SCNT fetuses. Furthermore, we sought appropriate mouse strains and cell types as nuclear donors to increase their developmental efficiencies and expand their applications in various fields. Peripheral blood cells are useful as ethical and economical cell species because they can be collected easily, even though SCNT embryos derived from hematopoietic cells show poor developmental abilities after reconstruction. Additionally, it is possible to obtain mice that are reactive to specific antigens of interest by using lymphocytes. Although there are still many limitations to the practical use of SCNT, its utilization is steadily expanding.

体细胞核移植(SCNT)是将体细胞转移到去核卵母细胞中产生个体的唯一生殖技术。尽管自首次在绵羊中报道哺乳动物SCNT以来已经过去了25年,但低出生率和形态异常等问题仍然存在,并限制了其实际应用。小鼠是揭示这些问题的理想实验动物,因为它具有成熟的生殖技术和广泛的基因组和各种菌株的知识基础。我们研究了供体体细胞核移植后不完全重编程的原因,发现一些胎盘特异性印迹基因的印记缺失可能导致非随机SCNT异常。通过改善异常表达的印迹基因,我们成功地提高了低出生率,改善了SCNT胎儿中观察到的形态异常。此外,我们寻找合适的小鼠品系和细胞类型作为核供体,以提高其发育效率并扩大其在各个领域的应用。外周血细胞作为一种伦理和经济的细胞种类是有用的,因为它们易于收集,尽管造血细胞衍生的SCNT胚胎在重建后表现出较差的发育能力。此外,利用淋巴细胞获得对特定抗原有反应的小鼠也是可能的。尽管SCNT在实际应用中仍有许多限制,但其应用正在稳步扩大。
{"title":"Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.","authors":"Kimiko Inoue","doi":"10.1262/jrd.2022-105","DOIUrl":"https://doi.org/10.1262/jrd.2022-105","url":null,"abstract":"<p><p>Somatic cell nuclear transfer (SCNT) is the only reproductive technology used to produce individuals from somatic cells by transferring them to enucleated oocytes. Although more than 25 years have passed since the first mammalian SCNT was reported in sheep, problems such as low birth rates and morphological abnormalities have persisted and limited its practical applications. The mouse is the ideal laboratory animal to unveil these questions due to its established reproductive technologies and extensive knowledge base of its genome and various strains. We investigated the causes of incomplete reprogramming after nuclear transfer of donor somatic cells and found that the loss of imprint in some placenta-specific imprinted genes could induce non-random SCNT abnormalities. By ameliorating aberrantly expressed imprinted genes, we succeeded in increasing the low birth rate and improving morphological abnormalities observed in SCNT fetuses. Furthermore, we sought appropriate mouse strains and cell types as nuclear donors to increase their developmental efficiencies and expand their applications in various fields. Peripheral blood cells are useful as ethical and economical cell species because they can be collected easily, even though SCNT embryos derived from hematopoietic cells show poor developmental abilities after reconstruction. Additionally, it is possible to obtain mice that are reactive to specific antigens of interest by using lymphocytes. Although there are still many limitations to the practical use of SCNT, its utilization is steadily expanding.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"129-138"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e4/3d/jrd-69-129.PMC10267587.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9633469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progesterone increases the success of in vitro fertilization via enhanced sperm hyperactivation in mice. 黄体酮通过增强小鼠精子的超激活来提高体外受精的成功率。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2022-114
Risa Suzuki, Masakatsu Fujinoki

Progesterone (P) enhances spermatozoal hyperactivation, a capacitation event. Hyperactivation is associated with successful in vitro fertilization (IVF). In this study, we examined the effects of P on hyperactivation and IVF in mice. P enhanced spermatozoal hyperactivation and increased IVF success rate in a dose-dependent manner. Moreover, P affected spermatozoal hyperactivation and IVF through the membrane progesterone receptor of the spermatozoal head. These results show that P regulates spermatozoal capacitation and fertilization in mice. The concentration of P changes during the estrous cycle, indicating that spermatozoa are capacitated in response to the oviductal environment and subsequently fertilize the oocyte.

黄体酮(P)增强精子过度活化,这是一种能化事件。过度激活与成功的体外受精(IVF)有关。在本研究中,我们检测了P对小鼠过度激活和体外受精的影响。P以剂量依赖性的方式增强精子的超激活,提高体外受精成功率。此外,P通过精子头部的膜孕酮受体影响精子的过度激活和体外受精。上述结果表明,磷对小鼠精子获能和受精具有调控作用。P的浓度在发情周期中发生变化,表明精子对输卵管环境作出反应,并随后使卵母细胞受精。
{"title":"Progesterone increases the success of in vitro fertilization via enhanced sperm hyperactivation in mice.","authors":"Risa Suzuki,&nbsp;Masakatsu Fujinoki","doi":"10.1262/jrd.2022-114","DOIUrl":"https://doi.org/10.1262/jrd.2022-114","url":null,"abstract":"<p><p>Progesterone (P) enhances spermatozoal hyperactivation, a capacitation event. Hyperactivation is associated with successful in vitro fertilization (IVF). In this study, we examined the effects of P on hyperactivation and IVF in mice. P enhanced spermatozoal hyperactivation and increased IVF success rate in a dose-dependent manner. Moreover, P affected spermatozoal hyperactivation and IVF through the membrane progesterone receptor of the spermatozoal head. These results show that P regulates spermatozoal capacitation and fertilization in mice. The concentration of P changes during the estrous cycle, indicating that spermatozoa are capacitated in response to the oviductal environment and subsequently fertilize the oocyte.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"147-153"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/20/jrd-69-147.PMC10267584.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9991849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysophosphatidic acid stimulates rat uterine contraction in vitro. 溶血磷脂酸刺激体外大鼠子宫收缩。
IF 1.8 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-06 DOI: 10.1262/jrd.2023-011
Satoshi Nagashima, Takuma Kimura, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu

Lysophosphatidic acid (LPA) has been implicated in the uterine endometrial functions of implantation and decidualization; however, not much is known about its myometrial contractile function. Herein we characterized the uterotonic effects of LPA in non-pregnant (estrus) and peri-parturient rats in vitro. LPA dose-dependently (0.01-10 μM) stimulated the amplitude and integral, but not the frequency, of the uterine strip contraction of estrous rats. The stimulatory effect of LPA was enhanced 1 day before parturition but was lost 1 day postpartum. LPA did not cause the de novo synthesis of prostaglandin (PG) F2α but stimulated contractions cooperatively with the PG. LPA-induced contractions were significantly inhibited by an LPA1/2/3 antagonist in the uteri of estrous rats but not in term rats. This study characterized the uterotonic effect of a natural LPA that occurs at physiological concentrations, changes with reproductive states, and is independent of mediation by the newly synthesized PG.

溶血磷脂酸(LPA)与子宫内膜着床和去胎化功能有关;然而,其肌层收缩功能尚不清楚。我们在体外研究了LPA对未怀孕(发情)和围分娩大鼠的子宫扩张作用。LPA剂量依赖性(0.01 ~ 10 μM)刺激发情大鼠子宫条形收缩的幅度和积分,但不影响频率。LPA的刺激作用在产前1天增强,产后1天减弱。LPA不引起前列腺素F2α的重新合成,但与PG协同刺激收缩,LPA1/2/3拮抗剂在发情大鼠子宫内明显抑制LPA诱导的收缩,而在足月大鼠子宫内无明显抑制作用。本研究描述了天然LPA在生理浓度下的子宫张力效应,随着生殖状态的变化而变化,并且不依赖于新合成的PG的介导。
{"title":"Lysophosphatidic acid stimulates rat uterine contraction in vitro.","authors":"Satoshi Nagashima,&nbsp;Takuma Kimura,&nbsp;Ryota Terashima,&nbsp;Makoto Sugiyama,&nbsp;Keiichiro Kizaki,&nbsp;Mitsumori Kawaminami,&nbsp;Shiro Kurusu","doi":"10.1262/jrd.2023-011","DOIUrl":"https://doi.org/10.1262/jrd.2023-011","url":null,"abstract":"<p><p>Lysophosphatidic acid (LPA) has been implicated in the uterine endometrial functions of implantation and decidualization; however, not much is known about its myometrial contractile function. Herein we characterized the uterotonic effects of LPA in non-pregnant (estrus) and peri-parturient rats in vitro. LPA dose-dependently (0.01-10 μM) stimulated the amplitude and integral, but not the frequency, of the uterine strip contraction of estrous rats. The stimulatory effect of LPA was enhanced 1 day before parturition but was lost 1 day postpartum. LPA did not cause the de novo synthesis of prostaglandin (PG) F<sub>2</sub>α but stimulated contractions cooperatively with the PG. LPA-induced contractions were significantly inhibited by an LPA1/2/3 antagonist in the uteri of estrous rats but not in term rats. This study characterized the uterotonic effect of a natural LPA that occurs at physiological concentrations, changes with reproductive states, and is independent of mediation by the newly synthesized PG.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 3","pages":"163-169"},"PeriodicalIF":1.8,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/85/jrd-69-163.PMC10267583.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9992339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Reproduction and Development
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1