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Evaluation of biofertilization on antimicrobial activity and phytochemical profile of Moringa olifera plant 生物施肥对辣木抗微生物活性及植物化学特性的影响
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275785
soha zalat, Samia H. Abou zekry, Afaf Ali Amin, Maha Amin Hewedy
Pot experiment was carried out during May 2019 at private farm in el Manyial, Cairo, Egypt. The investigation target was to find out the difference between using plant growth promoting rhizobacteria (B. cereus, Streptomyces chibaensis, B. megaterium, B. polymyxa) either individual or in combination with each other and mineral fertilization on antimicrobial activity and some phytochemical constitutes of Moringa olifera leaves. The highest values of antimicrobial activity recorded for mineral fertilization on treatment followed by mixed biofertilization treatment (GP4) (B. megaterium, B. polymyxa) against seven foodborne pathogens Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus stolonifer and Botrytis cinera, as compared with the control treatment. Biofertilization treatment (B. megaterium, B. polymyxa) recorded increasing over control in (GP10) by 160.8% and decreasing of chemical fertilization by 5.41% for polyphenol content, also, the antioxidant content shown increasing over the control (GP10) by 81.1% and decreasing of chemical fertilization (GP9) by 5.6% as well as vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 55 days. Biofertilization in (GP4) recorded increasing over control group (GP10) by 48.89%, 27.08% and 58% for vitamin A, C and chlorophyll respectively. Ethyl acetate extract was found to be most potent extract than ethanol and water against all tested microorganism.
盆栽试验于2019年5月在埃及开罗el Manyial的私人农场进行。本研究旨在了解促进植物生长的根瘤菌(蜡状芽孢杆菌、芝巴链霉菌、巨型芽孢杆菌、多粘芽孢杆菌)单独或联合施用与矿物施肥对辣木叶片抑菌活性及部分植物化学成分的影响。与对照处理相比,矿物施肥处理(GP4)(巨型芽孢杆菌、多粘芽孢杆菌)对大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌、铜绿假单胞菌、黑曲霉、stolonifer根霉和葡萄球菌等7种食源性致病菌的抗菌活性最高。生物肥处理(megaterium B., polymyxa B.)的多酚含量比对照(GP10)增加了160.8%,化学肥处理减少了5.41%,抗氧化剂含量比对照(GP10)增加了81.1%,化学肥处理(GP9)减少了5.6%,无机肥和生物肥处理(GP4)在55 d后增加了维生素C和A。生物肥处理(GP4)的维生素A、C和叶绿素含量比对照组(GP10)分别提高了48.89%、27.08%和58%。结果表明,乙酸乙酯提取物比乙醇和水提取物对所有微生物的抑制作用最强。
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引用次数: 0
Synthesis and antimicrobial study of new tetrazole, benzimidazole and N3-functionalized quinazolinone derivatives 新型四唑、苯并咪唑及n3功能化喹唑啉酮衍生物的合成与抗菌研究
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275777
Dina G. Sanad, Ahmed S.A. Youssef, Fatma A. El-Mariaha, Heba E. Hashem
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引用次数: 0
Anticandidal Activity of Green Synthesized Zinc Oxide Nanoparticles Using Lemon Peel Extract 柠檬皮提取物合成绿色氧化锌纳米颗粒的抗药活性研究
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275788
Reham Metwally, Zeinab M.H. Kheiralla, Sanaa M. Ashour, Sanaa S. Zaki
Nanobiotechnology has developed as an effective technology for developing antimicrobial nanoparticles in an environmentally safe manner. In this study, green synthesized zinc oxide nanoparticles (ZnO NPS) from zinc acetate solution by using lemon peels aqueous extract was characterized by UV–Visible Spectroscopy, High-resolution Transmission Electron Microscopy (HR-TEM) and Dynamic Light Scattering (DLS). Anticandidal activity was investigated against three clinical multidrug resistant Candida species including two Candida albicans, one Candida glabrata and one Candida krusei using four antifungal agents by disc diffusion method and antifungal activity of ZnO NPS was assayed by disc diffusion method and determination of the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Characterization studies revealed that the synthesized nanoparticles have rod shape with sizes of 13.58 30.70 nm. Notably, high rates of resistance were observed with the four tested antifungal agents against all Candida species and the antifungal activity of the synthesized ZnO NPS against Candida species were exhibited, with a maximum zone of inhibition of 24.5±0.5 mm against C. glabrata followed by C. albicans (19.5±0.5 mm) and C. krusei (16.0±0.0 mm). MIC and MFC for all Candida species were 0.25 and 0.5 mg/ml respectively. The cytotoxic data indicates that ZnO NPs have half maximal inhibitory concentration (IC50) value = 230.12 ± 9.34 μg/ml on normal human lung fibroblast cell line (MRC5). In conclusion, the study elucidates that lemon peels mediated green synthesized zinc oxide nanoparticles have antifungal activity against different Candida species. So that it can be developed as a novel medicine for the treatment of Candida associated infections in the near future.
纳米生物技术已经发展成为一种有效的以环境安全的方式开发抗菌纳米颗粒的技术。采用紫外可见光谱、高分辨率透射电镜(HR-TEM)和动态光散射(DLS)对柠檬皮水提液中乙酸锌溶液合成的绿色氧化锌纳米粒子(ZnO NPS)进行了表征。采用圆盘扩散法研究了4种抗真菌药物对3种临床多重耐药念珠菌(2种白色念珠菌、1种光面念珠菌和1种克氏念珠菌)的抑菌活性,采用圆盘扩散法测定了ZnO NPS的抑菌活性,并测定了最小抑菌浓度(MIC)和最小杀真菌浓度(MFC)。表征研究表明,合成的纳米颗粒具有棒状,尺寸为13.58 ~ 30.70 nm。结果表明,合成的氧化锌NPS对假丝酵母菌均有较高的耐药率,对光滑假丝酵母菌的最大抑制区为24.5±0.5 mm,其次是白色假丝酵母菌(19.5±0.5 mm)和克鲁西假丝酵母菌(16.0±0.0 mm)。所有念珠菌的MIC和MFC分别为0.25和0.5 mg/ml。结果表明,ZnO NPs对正常人肺成纤维细胞株(MRC5)的半数最大抑制浓度(IC50)为230.12±9.34 μg/ml。综上所述,本研究阐明了柠檬皮介导的绿色合成氧化锌纳米颗粒对不同种类的念珠菌具有抗真菌活性。因此,在不久的将来,它可以作为一种治疗念珠菌相关感染的新药。
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引用次数: 0
Compаrаtive study between Volumetric Modulаted Аrc therаpy plаns using FF аnd FFF beаm in cаse of heаd аnd neck cаncer 使用FF和FFF波束的体积模块化Аrc治疗方案在头颈部癌症治疗中的比较研究
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275772
Aly Elamrawy, E. Attalla, Tarek Eldsoky
This study аims to evаluаte the dosimetric аnd biologicаl differences in Rаdiotherаpy protocol аt 6MV photon from flаttened аnd flаttening filter free (FFF) using Volumetric Modulаted Аrc therаpy (VMАT) beаm in patient diagnosed with Heаd аnd Neck cаncer. To establish this aim ten pаtients with squаmous cell cаrcinomа were subjected to 20 VMАT plаns using Monаco 5.51.10 treаtment plаnning system (TPS) at 6MV FF аnd 6MV FFF . The quаlity of plаn аnd efficiency were evаluаted using radiobiolgical parameters (NTCP) normаl tissue complicаtion probаbility and (TCP) tumor control probаbility. The results showed thаt there is difference between 6FF аnd 6FFF in TCP vаlues, while the target coverage and sparing of the OARs for FFF VMAT were similar to those for FF VMAT. Also, there was no observable differences in homogeneity or conformity index for both modes plаns. Conclusively, all treatment protocols met the planning objectives and 6 MV FFF-VMAT plan is a highly efficient and feasible option for the treatment of head and neck cancer .
本研究旨在利用Volumetric modulsted Аrc therpy (VMАT) beam评估被诊断为肺癌的患者中来自flata和flata滤光器(FFF)的rta协议的6MV光子的剂量学和生物学差异。为了建立这一目标,10名患有squsquous细胞癌的患者在6MV FF和6MV FFF下,使用monda5.51.10治疗方案(TPS)进行20次VMАT plnas治疗。使用放射生物学参数(NTCP)正常组织并发症概率和(TCP)肿瘤控制概率来评估肿瘤质量和效率。结果表明,6FF和6FFF在TCP vlulues中存在差异,而FFF VMAT的桨的目标覆盖率和节省量与FF VMAT相似。此外,两种模式的同质性或一致性指数没有明显差异。综上所述,所有治疗方案均达到了计划目标,6mv FFF-VMAT方案是治疗头颈癌的一种高效可行的方案。
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引用次数: 0
Energy staggering in superdeformed bands for isodiaphere nuclei in A ~ 40-60 mass region A ~ 40-60质量区等绝缘核的超变形能带能量交错
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275774
Eman R. Abo Elyazeed, Hager H. Soudii, D. A. Elrayen, H. Yassin
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引用次数: 0
Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water 农业排水中分离的芽孢杆菌FYM31降解有机磷农药(马拉硫磷)的研究
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275794
walaa Madbolly, Manal F. Abdelall, Sanaa S. Zaki, Hanan A. Nour El-Din, Mona I. Fahd, Soad A. Abdallah
Organophosphorus pesticides (OP) are used extensively in many arenas including agriculture and industry leading to humans and agroecosystems disorders. Malathion is one of the OP that are used in agriculture to control pest and protect crops. Also, they harm non-target organisms and affect cruelly water sources, air, and soil quality. The present study aimed to isolate and identify a potent bacterial isolate capable of degrading malathion. Bacterial strain that isolated from Al Fayoum governorate, Egypt exhibited high efficiency for malathion biodegradation. Biodegradation process using minimal salt medium (MSM) supplemented with different malathion concentrations indicated that the bacterium was able to degrade and use malathion as a sole carbon source up to 700 mg/l at 37°C.The potent strain that exhibited biodegradation potential was identified as Bacillus sp. FYM31 and deposited into GenBank with the accession number OK325597. HPLC proved the effectiveness of malathion removal by Bacillus sp. FYM31 after 12 days of incubation to the level of 70.1% malathion (700 mg/l) degradation. Organophosphorus hydrolase (opd) gene was detected in the potent Bacillus sp. FYM31 strain. Due to the widespread usage of malathion in Egypt's agricultural areas, Bacillus sp. FYM31 can help bio-remediate the polluted areas.
有机磷农药(OP)广泛应用于包括农业和工业在内的许多领域,导致人类和农业生态系统失调。马拉硫磷是农业中用于防治病虫害和保护作物的有机磷农药之一。此外,它们还会伤害非目标生物,并严重影响水源、空气和土壤质量。本研究旨在分离和鉴定一种能够降解马拉硫磷的有效细菌分离物。从埃及Al Fayoum省分离的菌株对马拉硫磷具有较高的生物降解效率。在添加不同浓度马拉硫磷的低盐培养基(MSM)中进行的生物降解过程表明,该细菌能够在37°C下降解并将马拉硫磷作为唯一碳源,最高可达700 mg/l。菌株鉴定为芽孢杆菌sp. FYM31,登录号为OK325597。高效液相色谱法证实,菌株FYM31对马拉硫磷的去除率达到70.1% (700 mg/l)。在强效芽孢杆菌FYM31株中检测到有机磷水解酶(opd)基因。由于马拉硫磷在埃及农业地区的广泛使用,芽孢杆菌sp. FYM31可以帮助污染地区进行生物修复。
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引用次数: 1
Study of Chargino-Nutralino Pair Production like Higgsinos and Gauginos at The LHC with Three Leptons Final State and Missing Transverse Momentum 三轻子末态和缺少横向动量的大型强子对撞机中Higgsinos和Gauginos等电荷-中微子对产生的研究
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275771
Nada Ezzelarab, Ahmed Ali Abdelalim, M. Sirag
The calculation of chargino-nutralino pair production at the LHC with √s = 14 TeV had been studied. Different benchmark points are evaluated for the chargino-nutralino pair production using MSSM for four conditions on mass parameters. The results were compared with the latest data recorded by the LHC at √(s) = 8, 13, 14 TeV for pp collisions. In the minimal supersymmetric extension of the SM (MSSM) the mass parameters for the bino, wino, and higgsino states represented by M1, M2, and μ. Our results depend on the nature of the chargino-nutralino pair production and its masses. In case of light higgsino like sleptons the total cross section produced at small x, while for gauginos like s-quarks produced at large x due to the fact that gaugino constrained by CMS and ATLAS as heavier than 1 TeV. Assuming that the lightest supersymmetric particle (LSP) x ̃_1^0 is stable with R-parity conservation, it is a well-motivated to viable dark-matter candidate. Two scenarios of chargino-nutralino pair production are considered in this search. The first scenario Gaugino like with two conditions on the wino-bino and higgsino mass parameters [(M1 < M2 < μ) and (M1 < M2 < −μ)]. The second scenario Higgsino like with two conditions on the wino-bino and higgsino mass parameters [(M1 < −μ < M2) and (−μ < M1 < M2)]. Also, the effect of the sign and value of μ on the total cross sections for higgsinos and gauginos was represented. Nada Ezzelarab et al. J. Sci. Res. Sci., 2022, 39, (1): 31-46
研究了√s = 14 TeV的大型强子对撞机中电荷-中性子对产生的计算。在质量参数的四种条件下,对MSSM生产电荷-中性子对的不同基准点进行了评估。结果与LHC在√(s) = 8,13,14 TeV下记录的最新pp碰撞数据进行了比较。在SM的最小超对称扩展(MSSM)中,用M1、M2和μ表示的二能级、二能级和高能级的质量参数。我们的结果取决于电荷-中性子对产生的性质及其质量。对于轻的高介子,如slepton,总横截面在小x处产生,而对于高介子,如s-夸克,由于受CMS和ATLAS约束的高介子比1 TeV重,在大x处产生。假设最轻的超对称粒子(LSP) x ^ 1^0是稳定的,具有r宇称守恒,它是一个可行的暗物质候选者。在这个搜索中考虑了电荷-中性子对产生的两种情况。第一种情况下,高基诺具有两种条件,分别是wino-bino和higgsino质量参数[(M1 < M2 < μ)和(M1 < M2 <−μ)]。第二种情况是Higgsino,在wino-bino和Higgsino质量参数[(M1 <−μ < M2)和(−μ < M1 < M2)]两个条件下。此外,还讨论了μ的符号和值对高、高能粒子总截面的影响。Nada Ezzelarab等人。j .科学。研究科学。浙江农业学报,2022,39 (1):31-46
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引用次数: 0
Egyptian truffles as a source of antimicrobial and antioxidant agents 埃及松露作为抗菌和抗氧化剂的来源
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275787
F. Sallam, Mehreshan T. El- mokadem, Amal A. I. Mekawey, Ebtehag A.E Saker
Edible desert truffles are obligate symbiotic macro hypogenous ascomycetes fruit bodies. These are growing in depth between 5 and 10 cm. These consider a miracle of nature and an unexploited source of therapeutic compounds with antimicrobial, antioxidant, and wonderful food, especially for Bedouins. The current study investigated the activity of premature and mature ethyl acetate truffle extracts (white and Red) collected from the Western Egyptian Desert as antimicrobial and antioxidant. This study evaluated in vitro the efficacy of antimicrobial activity of organic truffles extract, and their effect on various pathogens (Gramnegative, Gram-positive bacteria, filamentous fungi, and yeast) by using agar well diffusion. TEM micrographs had been done for the most effective crude extracts. Furthermore, the activity of DPPH scavenging was studied for both mature truffles. Both truffles extracts had antibacterial activity more than antifungal activity. The selected extracts exhibited an inhibitory effect on the cell wall and protoplasm of pathogens. Terfezia sp. had antioxidant activity more than Tirmania sp. This investigation concluded that the truffle extracts could be considered a promising antibiotic and antioxidant drug in near future.
可食用的沙漠松露是专性共生的宏观下生子囊菌子实体。它们的生长深度在5到10厘米之间。这些都被认为是大自然的奇迹,是抗菌、抗氧化和美妙食物的治疗化合物的未开发来源,特别是对贝都因人来说。本研究对采自埃及西部沙漠的过早和成熟松露乙酸乙酯提取物(白色和红色)的抗菌和抗氧化活性进行了研究。本研究通过琼脂孔扩散法对有机松露提取物的体外抑菌效果进行了评价,并对多种病原菌(革兰氏阴性菌、革兰氏阳性菌、丝状真菌和酵母菌)的抑菌效果进行了研究。对最有效的粗提物进行了TEM显微观察。此外,还研究了两种成熟松露对DPPH的清除能力。两种松露提取物的抑菌活性均大于抗真菌活性。所选提取物对病原菌细胞壁和原生质均有抑制作用。特非齐亚菌的抗氧化活性明显高于蒂尔曼尼亚菌。本研究表明,松露提取物是一种很有前景的抗氧化药物。
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引用次数: 1
Asymptotic Iteration Method in Single and Multilayered Quantum Dots 单层和多层量子点的渐近迭代方法
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.112618.1067
I. Mikhail, Heba Fahim
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引用次数: 0
Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits 本地杀手酵母对巴拉迪橙果实采后病原菌的防治效果评价
Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275786
Doaa Abdel maksoud elfaramawy, Hoda H. Abo ghalia, Sanaa M. Ashour, Ahmed A. Mohamed, Sanaa S. Zaki
Yeasts are some of the most important postharvest biocontrol agents (BCAs). Postharvest oranges frequently deteriorate due to green and blue moulds, leading to significant economic losses. The purposes of the present study were to isolate blue and green moulds from infected orange fruits, to assess the ability of killer yeasts isolated from healthy orange fruits and leaves from orange orchards to control blue and green moulds and to evaluate the additive effect of BCAs in combination with 2% sodium bicarbonate (SBC), 2%, sodium benzoate (SB), 2% calcium chloride, 0.2% salicylic acid (SA) or 0.5% chitosan. Among eight fungi isolated from orange fruits showing symptoms of green and blue mulds infection, two were identified as P. digitatum and P. italicum and selected for in vitro assays. Twenty eight yeast isolates were obtained from orange leaves and from the surface of fruits. All yeasts exhibited high killer activity. Twelve yeasts reduced 22.5 –70% of P. digitatum growth while seven isolates reduced 21.168.5% of P. italicum growth. The most potent yeast isolates were identified as Candida pseudotropicalis, Candida salmanticensis, Candida membranifaciens and Pichia guilliermondii. Combination of the BCAs, C. pseudotropicalis, C. salmanticensis and P. guilliermondii with SBC, CaCl2 or chitosan increased their effectiveness against P. digitatum. While combination of C. pseudotropicalis, C. membranifaciens and P. guilliermondii with these natural compounds decreased their effectiveness against P. italicum. Combination of C. membranifaciens with SA increased its effectiveness against P. digitatum. Sodium benzoate has additive effect on C. pseudotropicalis against P. digitatum and C. pseudotropicalis and P. guilliermondii against P. italicum.
酵母是最重要的采后生物防治剂之一。采后的橙子经常因绿霉和蓝霉而变质,造成重大的经济损失。本研究的目的是从受侵染的柑桔果实中分离出蓝霉和绿霉,评价从柑桔健康果实和叶片中分离出的杀菌对蓝霉和绿霉的控制能力,并评价bcaa与2%碳酸氢钠(SBC)、2%苯甲酸钠(SB)、2%氯化钙、0.2%水杨酸(SA)或0.5%壳聚糖联合使用的加性效果。从橙果中分离到的8种真菌中,有绿霉菌和蓝霉菌感染的症状,其中2种真菌被鉴定为digitatum和P. italicum,并选择用于体外测定。从橙叶和果实表面分离得到28株酵母菌。所有酵母均表现出较高的杀伤活性。12株酵母对指状假单胞菌生长的抑制作用为22.5 ~ 70%,7株酵母对意大利假单胞菌生长的抑制作用为21.168.5%。鉴定出最强的酵母菌分离株为假热带假丝酵母菌、沙曼假丝酵母菌、膜性假丝酵母菌和吉利蒙毕赤酵母。bca与SBC、CaCl2或壳聚糖联合使用,对假热带弓形虫、萨尔曼弓形虫和吉利蒙地弓形虫的防治效果增强。假热带假丝酵母菌、膜性假丝酵母菌和吉列蒙地假丝酵母菌与这些天然化合物合用时,对意大利假丝酵母菌的防治效果下降。膜化棘球蚴与SA联合使用可提高其对指状棘球蚴的防治效果。苯甲酸钠对假热带弓形虫对指状弓形虫和假热带弓形虫对意大利弓形虫有加性作用。
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引用次数: 0
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Journal of Scientific Research in Science
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