Pub Date : 2022-12-14DOI: 10.20473/jscrte.v6i2.42757
Andi Nur Ummah A. Nur Ummah
Objective to determine the effect of triamcinolone acetonide as an antifibrotic agent on inflammatory response and collagen type I after strabismus surgery in rabbit Material and methods thirty six eyes of male New Zealand white rabbits divided by two groups, 18 rabbits eyes in control group and 18 rabbits eyes in treatment group. Control group underwent recess muskulus rektus superior and irrigation of balanced salt solution in reattachment site. Treatment group underwent strabismus surgery and irrigation Triamcinolone Acetonide (TCA) (Flamicort ® Dexa-medica)(40 mg/ml) 0,15 ml (6 mg). They were terminated on 15 postoperative days. Staining Hematoxylin & Eosin were performed to evaluate inflammatory response and immunohistochemistry using a monoclonal antibody Collagen I Alpha 2 Antibody (LS-C352030, LifeSpan BioSciences, Inc) was performed to evaluate collagen type I expression. Results this study showed of inflammatory response decreased and statistically significant in the treatment group (p=0.002, p=<0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p=0.004, p<0.05). Conclusion triamcinolone Acetonide (TCA) 40 mg/ml is one of therapeutic approaches that aims to reduce fibrosis after strabismus surgery by inhibiting the accumulation of inflammatory cells activation and suppressing of type I collagen deposition.
{"title":"TRIAMCINOLONE ACETONIDE EFFECT ON INFLAMMATORY RESPONSE AND EXPRESSION OF COLLAGEN TYPE I AFTER STRABISMUS SURGERY","authors":"Andi Nur Ummah A. Nur Ummah","doi":"10.20473/jscrte.v6i2.42757","DOIUrl":"https://doi.org/10.20473/jscrte.v6i2.42757","url":null,"abstract":"Objective to determine the effect of triamcinolone acetonide as an antifibrotic agent on inflammatory response and collagen type I after strabismus surgery in rabbit Material and methods thirty six eyes of male New Zealand white rabbits divided by two groups, 18 rabbits eyes in control group and 18 rabbits eyes in treatment group. Control group underwent recess muskulus rektus superior and irrigation of balanced salt solution in reattachment site. Treatment group underwent strabismus surgery and irrigation Triamcinolone Acetonide (TCA) (Flamicort ® Dexa-medica)(40 mg/ml) 0,15 ml (6 mg). They were terminated on 15 postoperative days. Staining Hematoxylin & Eosin were performed to evaluate inflammatory response and immunohistochemistry using a monoclonal antibody Collagen I Alpha 2 Antibody (LS-C352030, LifeSpan BioSciences, Inc) was performed to evaluate collagen type I expression. Results this study showed of inflammatory response decreased and statistically significant in the treatment group (p=0.002, p=<0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p=0.004, p<0.05). Conclusion triamcinolone Acetonide (TCA) 40 mg/ml is one of therapeutic approaches that aims to reduce fibrosis after strabismus surgery by inhibiting the accumulation of inflammatory cells activation and suppressing of type I collagen deposition.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85116127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.20473/jscrte.v6i2.42759
Sekar Ayu Sitoresmi Sekar Ayu
Objective to analyze the effect of bevacizumab on α-smooth muscle actin expression and fibroblast count in trabeculectomy area of rabbit models in order to find a safer modulator of wound healing to improve surgical outcome. Material and methods 16 New Zealand white rabbits aged 4-6 months and weigh between 2,5-3,5 kg were performed trabeculectomy in their right eyes with postoperative subconjunctival injection of BSS and Bevacizumab. Rabbits were put into control and bevacizumab group using simple random sampling. Examination were done postoperative day 1, 7, and 14 and subjects were terminated and performed enucleation on postoperative day 14. The samples were histologically stained with Haematoxyline-Eosin to count the fibroblast and immunohistochemistry using α-smooth muscle actin antibody to differ the myofibroblast from fibroblast and the expression of α-SMA were collected using immunoreactive score. Result Mann Whitney u test and independent t-test were used to analyze the data, and we found both less expression of α-smooth muscle actin and fibroblast count on bevacizumab group compared to control group which indicates less myofibroblast, fibroblast, and less scarring potential in trabeculectomy area. Conclusion bevacizumab inhibits fibroblast proliferation and its differentiation to myofibroblast that lead to less collagen production and fibrosis.
{"title":"EFFECT OF BEVACIZUMAB ON α-SMOOTH MUSCLE ACTIN EXPRESSION AND FIBROBLAST COUNT IN TRABECULECTOMY AREA TOWARDS PREVENTION OF FIBROSIS","authors":"Sekar Ayu Sitoresmi Sekar Ayu","doi":"10.20473/jscrte.v6i2.42759","DOIUrl":"https://doi.org/10.20473/jscrte.v6i2.42759","url":null,"abstract":"Objective to analyze the effect of bevacizumab on α-smooth muscle actin expression and fibroblast count in trabeculectomy area of rabbit models in order to find a safer modulator of wound healing to improve surgical outcome. Material and methods 16 New Zealand white rabbits aged 4-6 months and weigh between 2,5-3,5 kg were performed trabeculectomy in their right eyes with postoperative subconjunctival injection of BSS and Bevacizumab. Rabbits were put into control and bevacizumab group using simple random sampling. Examination were done postoperative day 1, 7, and 14 and subjects were terminated and performed enucleation on postoperative day 14. The samples were histologically stained with Haematoxyline-Eosin to count the fibroblast and immunohistochemistry using α-smooth muscle actin antibody to differ the myofibroblast from fibroblast and the expression of α-SMA were collected using immunoreactive score. Result Mann Whitney u test and independent t-test were used to analyze the data, and we found both less expression of α-smooth muscle actin and fibroblast count on bevacizumab group compared to control group which indicates less myofibroblast, fibroblast, and less scarring potential in trabeculectomy area. Conclusion bevacizumab inhibits fibroblast proliferation and its differentiation to myofibroblast that lead to less collagen production and fibrosis.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81351628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.20473/jscrte.v6i2.42774
Rini Riyanti
The excess of unbound α globin chains are the basic pathophysiology of the cause of clinical symptoms in major β thalassemia. Recently there are many alternative therapies by increasing γ globin chains to reduced the effects of unbound α globin chains. Alternative therapies by decreasing α globin chains have not been much noticed. α globin expression involves complex regulatory involving transcription factors and miRNAs. It involves GATA-1, KLFD and MYB transcription factors . It also involves miRNA-144 and miRNA-150. The role of miRNA-144 and miRNA-150 to reduce the α globin chains expression in major β thalassemia patients is an alternative therapy. miRNA-144 and miRNA150 activity need to be known by using anti-miRNA 144 and anti- miRNA 150. Analyzed the effect of anti-miRNA 144 and anti-miRNA 150 on the expression of α globin chains. This study was an experimental study using PBMC of a major β thalassemia patients. PBMC divided into four groups that were not transfected, transfected by anti-miRNA 144, transfected by anti-miRNA 150 and transfected by anti-miRNA 144 and anti-miRNA 150. qPCR examinations to find out expression of miRNA-144 and miRNA-150. Western blot examination to find out the expression of α globin chains. There was significantly lower miRNA-144 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 144 than those not transfected. In line with the result of miRNA-144 expression which was 0.17 times lower than the control group. There was significantly lower miRNA-150 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. In line with the result of miRNA-150 expression which was 0.30 times lower than the control group. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 and anti-miRNA 144 than those not transfected. This is evidenced by the decreased in the area 10-25 KDa band. Based on this study, the administration of anti-miRNA 150 or anti-miRNA 144 and anti-miRNA 150 is capable of decreasing the expression α globin chains in PBMC of major β thalassemia patients.
{"title":"EFFECT OF Anti-miRNA 144 AND Anti-miRNA 150 ON THE EXPRESSION OF α GLOBIN CHAINS IN PBMC","authors":"Rini Riyanti","doi":"10.20473/jscrte.v6i2.42774","DOIUrl":"https://doi.org/10.20473/jscrte.v6i2.42774","url":null,"abstract":"The excess of unbound α globin chains are the basic pathophysiology of the cause of clinical symptoms in major β thalassemia. Recently there are many alternative therapies by increasing γ globin chains to reduced the effects of unbound α globin chains. Alternative therapies by decreasing α globin chains have not been much noticed. α globin expression involves complex regulatory involving transcription factors and miRNAs. It involves GATA-1, KLFD and MYB transcription factors . It also involves miRNA-144 and miRNA-150. The role of miRNA-144 and miRNA-150 to reduce the α globin chains expression in major β thalassemia patients is an alternative therapy. miRNA-144 and miRNA150 activity need to be known by using anti-miRNA 144 and anti- miRNA 150. Analyzed the effect of anti-miRNA 144 and anti-miRNA 150 on the expression of α globin chains. This study was an experimental study using PBMC of a major β thalassemia patients. PBMC divided into four groups that were not transfected, transfected by anti-miRNA 144, transfected by anti-miRNA 150 and transfected by anti-miRNA 144 and anti-miRNA 150. qPCR examinations to find out expression of miRNA-144 and miRNA-150. Western blot examination to find out the expression of α globin chains. There was significantly lower miRNA-144 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 144 than those not transfected. In line with the result of miRNA-144 expression which was 0.17 times lower than the control group. There was significantly lower miRNA-150 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. In line with the result of miRNA-150 expression which was 0.30 times lower than the control group. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 and anti-miRNA 144 than those not transfected. This is evidenced by the decreased in the area 10-25 KDa band. Based on this study, the administration of anti-miRNA 150 or anti-miRNA 144 and anti-miRNA 150 is capable of decreasing the expression α globin chains in PBMC of major β thalassemia patients.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77477545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.20473/jscrte.v6i2.42773
Diana Yuliawati
Objective to examine the effect of Bevacizumab injection to the angiogenesis which the amount and density of blood vessel as the indicators after trabeculectomy procedure. Method This was a true experimental study using 16 eyes of 16 New Zealand White Rabbit eye treated by trabeculectomy procedure with eight eyes as the control group using Balanced Saline Solution (BSS) and eight eyes as the treatment group using Bevacizumab. It was injected subconjuctiva after the trabeculectomy. At the end of the study all rabbits in each group was sacrified, the eye was enucleated and the bleb area was dissected, and then processed for histological studies. The amount and density of blood vessel were evaluated using haematoxyllin eosin methode at day 14 after the eyes was done for trabeculectomy procedure. Result The mean of amount of blood vessel in control group was 22,63 ± 11,02 and treatment group was 14,75 ± 4,92 (p=0.043). The mean of density of blood vessel in control group was 19,10 ± 1,69 % and treatment group was 16,53 ± 2,90 % (p=0.029)%. The result shows there were statistically significant difference between the two groups (p<0.05). Conclusion In this study the subconjunctival Bevacizumab injection after trabeculectomy reduce the amount and density of blood vessel compared with subconjunctival BSS injection only, thus it is potential in preventing subconjunctival fibrosis after trabeculectomy
{"title":"EFFECT OF BEVACIZUMAB ON ANGIOGENESIS INTRABECULECTOMY AREA","authors":"Diana Yuliawati","doi":"10.20473/jscrte.v6i2.42773","DOIUrl":"https://doi.org/10.20473/jscrte.v6i2.42773","url":null,"abstract":"Objective to examine the effect of Bevacizumab injection to the angiogenesis which the amount and density of blood vessel as the indicators after trabeculectomy procedure. Method This was a true experimental study using 16 eyes of 16 New Zealand White Rabbit eye treated by trabeculectomy procedure with eight eyes as the control group using Balanced Saline Solution (BSS) and eight eyes as the treatment group using Bevacizumab. It was injected subconjuctiva after the trabeculectomy. At the end of the study all rabbits in each group was sacrified, the eye was enucleated and the bleb area was dissected, and then processed for histological studies. The amount and density of blood vessel were evaluated using haematoxyllin eosin methode at day 14 after the eyes was done for trabeculectomy procedure. Result The mean of amount of blood vessel in control group was 22,63 ± 11,02 and treatment group was 14,75 ± 4,92 (p=0.043). The mean of density of blood vessel in control group was 19,10 ± 1,69 % and treatment group was 16,53 ± 2,90 % (p=0.029)%. The result shows there were statistically significant difference between the two groups (p<0.05). Conclusion In this study the subconjunctival Bevacizumab injection after trabeculectomy reduce the amount and density of blood vessel compared with subconjunctival BSS injection only, thus it is potential in preventing subconjunctival fibrosis after trabeculectomy","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83312835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.20473/jscrte.v6i2.42836
Shinta Shinta Arta Wiguna
Objective to determine the effect of bevacizumab as an antifibrotic agent on collagen density, collagen thickness and collagen type I after trabeculectomy in rabbit. Materials and methods : Sixteen male New Zealand white rabbits divided by two groups, 8 rabbits in control group and 8 rabbits in treatment group. Control group underwent trabeculectomy and injection of balanced salt solution. Treatment group underwent trabeculectomy and subconjunctival injection of bevacizumab (1.25mg, 25mg/mL). They were terminated on 14 postoperative days. Masson Trichrome were performed to evaluate collagen collagen density, collagen thickness. Immunohistochemistry using a monoclonal antibody to collagen type I was performed to evaluate collagen type I expression. Results This study showed the density of collagen fibers decreased and statistically significant in the treatment group (p = 0.075, p 0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p = 0.006, p<0.05). Conclusion Bevacizumab reduces bleb fibrosis by inhibition of angiogenesis and accumulation of extracellular matrix. Postoperative subconjunctival injection of bevacizumab may limiting scar tissue formation at the site of trabeculectomy by blocking collagen synthesis.
目的探讨贝伐单抗作为抗纤维化药物对兔小梁切除术后胶原密度、胶原厚度及胶原型的影响。材料与方法:雄性新西兰大白兔16只，随机分为两组，对照组8只，治疗组8只。对照组行小梁切除术并注射平衡盐溶液。治疗组行小梁切除术，结膜下注射贝伐单抗(1.25mg, 25mg/mL)。术后14天终止手术。马松三色法测定胶原蛋白密度、胶原蛋白厚度。采用I型胶原单克隆抗体免疫组化检测I型胶原表达。结果治疗组胶原纤维密度降低，差异有统计学意义(p = 0.075, p 0.05)。与BSS组相比，治疗组I型胶原表达降低(p = 0.006, p<0.05)。结论贝伐单抗通过抑制血管生成和细胞外基质的积累来减轻大疱性纤维化。术后结膜下注射贝伐单抗可以通过阻断胶原合成来限制小梁切除术部位瘢痕组织的形成。
{"title":"BEVACIZUMAB EFFECT ON EXPRESSION OF COLLAGEN TYPE I AFTER TRABECULECTOMY","authors":"Shinta Shinta Arta Wiguna","doi":"10.20473/jscrte.v6i2.42836","DOIUrl":"https://doi.org/10.20473/jscrte.v6i2.42836","url":null,"abstract":"Objective to determine the effect of bevacizumab as an antifibrotic agent on collagen density, collagen thickness and collagen type I after trabeculectomy in rabbit. Materials and methods : Sixteen male New Zealand white rabbits divided by two groups, 8 rabbits in control group and 8 rabbits in treatment group. Control group underwent trabeculectomy and injection of balanced salt solution. Treatment group underwent trabeculectomy and subconjunctival injection of bevacizumab (1.25mg, 25mg/mL). They were terminated on 14 postoperative days. Masson Trichrome were performed to evaluate collagen collagen density, collagen thickness. Immunohistochemistry using a monoclonal antibody to collagen type I was performed to evaluate collagen type I expression. Results This study showed the density of collagen fibers decreased and statistically significant in the treatment group (p = 0.075, p 0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p = 0.006, p<0.05). Conclusion Bevacizumab reduces bleb fibrosis by inhibition of angiogenesis and accumulation of extracellular matrix. Postoperative subconjunctival injection of bevacizumab may limiting scar tissue formation at the site of trabeculectomy by blocking collagen synthesis.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"359 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76550340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-12DOI: 10.20473/jscrte.v6i1.37512
D. Destri
Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, first is stem cells that have self renewal ability and multineage differentiation, second is bioreactor / growth factor, and then scaffold. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Purpose to prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum calcium levels. Methods: This study uses 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at 1st,3rd,7th, and 14th days which maxillary bone drilling only. While groups 5 to 8 were the positive control group at 1st,3rd,7th, and 14th days which were given chitosan scaffold. Groups 9 to 12 were treatment group at 1st,3rd,7th, and 14th days which were given hADMSC and chitosan scaffold. Blood collection is carried out in each group to check serum calcium levels. Result there were differences in calcium levels in blood serum in each group. Conclusion the application of hADMSC and chitosan scaffold caused a significant change in serum calcium levels on the 1st, 3rd, 7th and 14th days which meant that the combination of hADMSC and chitosan scaffold could trigger osteoinduction.
{"title":"Levels of Wistar Calcium Serum (Rattus Norvegicus) in Human Adipose-Derived Mesenchymal Stem Cells (hADMSCs) and Chitosan Scaffold by Osteoinduction Examination","authors":"D. Destri","doi":"10.20473/jscrte.v6i1.37512","DOIUrl":"https://doi.org/10.20473/jscrte.v6i1.37512","url":null,"abstract":"Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, first is stem cells that have self renewal ability and multineage differentiation, second is bioreactor / growth factor, and then scaffold. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Purpose to prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum calcium levels. Methods: This study uses 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at 1st,3rd,7th, and 14th days which maxillary bone drilling only. While groups 5 to 8 were the positive control group at 1st,3rd,7th, and 14th days which were given chitosan scaffold. Groups 9 to 12 were treatment group at 1st,3rd,7th, and 14th days which were given hADMSC and chitosan scaffold. Blood collection is carried out in each group to check serum calcium levels. Result there were differences in calcium levels in blood serum in each group. Conclusion the application of hADMSC and chitosan scaffold caused a significant change in serum calcium levels on the 1st, 3rd, 7th and 14th days which meant that the combination of hADMSC and chitosan scaffold could trigger osteoinduction.\u0000 ","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82536020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-12DOI: 10.20473/jscrte.v6i1.37516
W. Widya
Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding the teeth, including Human Gingival Fibroblasts (HGF) which is one of the major components of tissue formation in periodonsium. HGF regeneration with the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering using regenerative materials from cow or pig as the therapies, but these materials have some flaws so this research to find alternative materials regenerative tissue engineering scaffold collagen type 1 derived from fresh water fish scales, one of which are gourami fish scales. This research was conducted to test the viability of fish scales collagen gouramy against Human Gingival Fibroblasts for 24 hours. This study aimed to determine the concentration of fish scale collagen gourami which can maintain the viability of human gingival fibroblast cells for 24 hours. HGF is taken from healthy gingiva and planted in 96 well plates. Fish scales collagen gouramy with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells. The viability of HGF were increased after the addition of fish scales collagen gourami on concentration 0.32 mg/ml until 0.01 mg/ml. The highest viability of the cells was shown after the addition of 0.01 mg/ml. Fish scales collagen gouramy has the potential in tissue engeneering and the concentration of 0.01 mg/ml shows the highest viability of HGF.
{"title":"Viabilty Test of Fish Scales Collagen Gourami (Oshpronemus Gouramy) on Human Gingival Fibroblasts Cells","authors":"W. Widya","doi":"10.20473/jscrte.v6i1.37516","DOIUrl":"https://doi.org/10.20473/jscrte.v6i1.37516","url":null,"abstract":"Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding the teeth, including Human Gingival Fibroblasts (HGF) which is one of the major components of tissue formation in periodonsium. HGF regeneration with the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering using regenerative materials from cow or pig as the therapies, but these materials have some flaws so this research to find alternative materials regenerative tissue engineering scaffold collagen type 1 derived from fresh water fish scales, one of which are gourami fish scales. This research was conducted to test the viability of fish scales collagen gouramy against Human Gingival Fibroblasts for 24 hours. This study aimed to determine the concentration of fish scale collagen gourami which can maintain the viability of human gingival fibroblast cells for 24 hours. HGF is taken from healthy gingiva and planted in 96 well plates. Fish scales collagen gouramy with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells. The viability of HGF were increased after the addition of fish scales collagen gourami on concentration 0.32 mg/ml until 0.01 mg/ml. The highest viability of the cells was shown after the addition of 0.01 mg/ml. Fish scales collagen gouramy has the potential in tissue engeneering and the concentration of 0.01 mg/ml shows the highest viability of HGF.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74778337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-12DOI: 10.20473/jscrte.v6i1.37511
Ayu Kartika Fitri Ayu
Cervical cancer is a disease caused by a malignant process that occurs in the cervix or cervix. The cause of cervical cancer is not known for certain, but it is estimated that around 95% is caused by HPV (Human Papilloma Virus). Efforts to cure cancer with drugs (pharmacotherapy) or with chemical compounds (chemotherapy) in general have not been able to give satisfactory results, so alternative treatment methods are sought, including traditional medicine, namely by using mangroves. Lumnitzera racemosa is one type of mangrove plant that has been used in alternative medicine because of its potential as anticancer. The aim of this study was to determine the effect of Lumnitzera racemosa mangrove extract on hela cell viability. Lumnitzera racemosa leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 56 ppm, it means that the ethanol extract has toxic properties. The results of the phytochemical test of the leaf extract of Lumnitzera racemosa contained alkaloids, steroids, triterpenoids and saponins. The test results showed that the extract yield was 11.58%, the water content of the extract was 22.17%, and the total phenol was 2742.17 mg GAE. The test results from the LC-MS test resulted in suspected compounds including pyrogallol, isoniazid and caffeine. The ethanolic extract of Lumnitzera racemosa leaf was cytotoxic to the viability of hela cells with the resulting IC50 value of 493.33 µg/mL.
{"title":"Effect of Concentration of Mangrove Leaf Extract Lumnitzera Racemosa on Hela Cell Viability","authors":"Ayu Kartika Fitri Ayu","doi":"10.20473/jscrte.v6i1.37511","DOIUrl":"https://doi.org/10.20473/jscrte.v6i1.37511","url":null,"abstract":"Cervical cancer is a disease caused by a malignant process that occurs in the cervix or cervix. The cause of cervical cancer is not known for certain, but it is estimated that around 95% is caused by HPV (Human Papilloma Virus). Efforts to cure cancer with drugs (pharmacotherapy) or with chemical compounds (chemotherapy) in general have not been able to give satisfactory results, so alternative treatment methods are sought, including traditional medicine, namely by using mangroves. Lumnitzera racemosa is one type of mangrove plant that has been used in alternative medicine because of its potential as anticancer. The aim of this study was to determine the effect of Lumnitzera racemosa mangrove extract on hela cell viability. Lumnitzera racemosa leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 56 ppm, it means that the ethanol extract has toxic properties. The results of the phytochemical test of the leaf extract of Lumnitzera racemosa contained alkaloids, steroids, triterpenoids and saponins. The test results showed that the extract yield was 11.58%, the water content of the extract was 22.17%, and the total phenol was 2742.17 mg GAE. The test results from the LC-MS test resulted in suspected compounds including pyrogallol, isoniazid and caffeine. The ethanolic extract of Lumnitzera racemosa leaf was cytotoxic to the viability of hela cells with the resulting IC50 value of 493.33 µg/mL.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"242 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74899850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-12DOI: 10.20473/jscrte.v6i1.37514
F. Fauzan
Bone defect is one of the challenges for dentists in the process of healing bone tissue. Bone defect can occur in alveolar bone with the etiology of microorganisms and cyst expansion. In addition, cases of bone defects in alveolar bone are also often found in cases with treatment of apex resection and hemisection. Autologous bone graft is a clinical gold standard in the treatment of bone defect. However, the use of bone graft has a limited number of growth factors produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components; stem cell, growth factor, and scaffold. Stem cells will increase osteoblastogenesis and chitosan scaffold will immobilize alkaline phosphatase (ALP) so that serum ALP levels decrease and bone regeneration and mineralization processes become faster. The aim of this study is analyzing the effect of human adipose-derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in bone defect on serum alkaline phosphatase (ALP) levels. This research was a in vivo laboratory experimental study. Bone defects are planted with chitosan scaffold (CS) and a combination of human adipose-derived mesenchymal stem cells (HADMSC) with chitosan scaffold. Measurement of ALP levels was carried out by the International Federation of Clinical Chemistry (IFCC) method using an analyzer on the 1st, 3rd, 7th and 14th days. Research data were analyzed using multivariate analysis of variance (MANOVA) and Bonferroni tests. The results of the data analysis showed that there were significant differences in ALP levels with CS planting and the combination of HADMSC and CS. the effect of human adipose- derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) on bone defect reduces serum alkaline phosphatase (ALP) levels on the 3th and 14th days.
{"title":"Effect of Human Adipose-Derived Mesenchymal Stem Cell (HADMSC) With Chitosan Scaffold on Bone Defect White Rats (Rattus Norvegicus) on Serum Alkaline Phosphatase (ALP) Levels","authors":"F. Fauzan","doi":"10.20473/jscrte.v6i1.37514","DOIUrl":"https://doi.org/10.20473/jscrte.v6i1.37514","url":null,"abstract":"Bone defect is one of the challenges for dentists in the process of healing bone tissue. Bone defect can occur in alveolar bone with the etiology of microorganisms and cyst expansion. In addition, cases of bone defects in alveolar bone are also often found in cases with treatment of apex resection and hemisection. Autologous bone graft is a clinical gold standard in the treatment of bone defect. However, the use of bone graft has a limited number of growth factors produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components; stem cell, growth factor, and scaffold. Stem cells will increase osteoblastogenesis and chitosan scaffold will immobilize alkaline phosphatase (ALP) so that serum ALP levels decrease and bone regeneration and mineralization processes become faster. The aim of this study is analyzing the effect of human adipose-derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in bone defect on serum alkaline phosphatase (ALP) levels. This research was a in vivo laboratory experimental study. Bone defects are planted with chitosan scaffold (CS) and a combination of human adipose-derived mesenchymal stem cells (HADMSC) with chitosan scaffold. Measurement of ALP levels was carried out by the International Federation of Clinical Chemistry (IFCC) method using an analyzer on the 1st, 3rd, 7th and 14th days. Research data were analyzed using multivariate analysis of variance (MANOVA) and Bonferroni tests. The results of the data analysis showed that there were significant differences in ALP levels with CS planting and the combination of HADMSC and CS. the effect of human adipose- derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) on bone defect reduces serum alkaline phosphatase (ALP) levels on the 3th and 14th days.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76644398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-12DOI: 10.20473/jscrte.v6i1.37515
N. Azizah
The role of type I collagen is as a matrix of extracellular proteins with characteristics of increased cell proliferation which directly affects the physiology and morphology of cells. Type 1 collagen can be obtained either from fish scales. This is what underlies the author to support engineering tissue used for the treatment of periodontal disease in the regenerative field by utilizing collagen derived from gouramy scales. As an initial step, the researchers wanted to conduct a study using collagen extract derived from gouramy scales (Osphoronemus gouramy) which was applied to bone marrow mesenchymal stem cell cultures to see viability in vitro. To determine the viability of collagen in carp (Osphronemus goramy) scales to bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells are taken from mice and planted in 96 well plates. Collagen extracted from gouramy scales using the enzymatic method was dissolved in a condition medium and hydrolyzed into a collagen hydrolysis solution with each concentration of 0.01 mg / ml, 0.02 mg / ml, 0.04 mg / ml, 0.16 mg / ml, 0.32 mg / ml was put into the well prepared and incubated for 24 hours for the MTT assay. Collagen in carp scales can increase the viability of bone marrow mesenchymal stem cells with a percentage above 50% and the highest viability concentration at 0,01 mg / ml. The collagen of gouramy scales soaked in a medium condition has better viability than the collagen hydrolysis solution of carp. Collagen in carp scales is viable against bone marrow mesenchymal stem cells. Collagen scales of gouramy soaked in medium had the highest viability with an optimum dose of 0.01 mg / ml.
{"title":"Viabilty Test of Fish Scale Collagen (Oshpronemus Gouramy) on Bone Marrow Mesenchymal Stem Cell Culture","authors":"N. Azizah","doi":"10.20473/jscrte.v6i1.37515","DOIUrl":"https://doi.org/10.20473/jscrte.v6i1.37515","url":null,"abstract":"The role of type I collagen is as a matrix of extracellular proteins with characteristics of increased cell proliferation which directly affects the physiology and morphology of cells. Type 1 collagen can be obtained either from fish scales. This is what underlies the author to support engineering tissue used for the treatment of periodontal disease in the regenerative field by utilizing collagen derived from gouramy scales. As an initial step, the researchers wanted to conduct a study using collagen extract derived from gouramy scales (Osphoronemus gouramy) which was applied to bone marrow mesenchymal stem cell cultures to see viability in vitro. To determine the viability of collagen in carp (Osphronemus goramy) scales to bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells are taken from mice and planted in 96 well plates. Collagen extracted from gouramy scales using the enzymatic method was dissolved in a condition medium and hydrolyzed into a collagen hydrolysis solution with each concentration of 0.01 mg / ml, 0.02 mg / ml, 0.04 mg / ml, 0.16 mg / ml, 0.32 mg / ml was put into the well prepared and incubated for 24 hours for the MTT assay. Collagen in carp scales can increase the viability of bone marrow mesenchymal stem cells with a percentage above 50% and the highest viability concentration at 0,01 mg / ml. The collagen of gouramy scales soaked in a medium condition has better viability than the collagen hydrolysis solution of carp. Collagen in carp scales is viable against bone marrow mesenchymal stem cells. Collagen scales of gouramy soaked in medium had the highest viability with an optimum dose of 0.01 mg / ml.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80562237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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