Journal of The Chinese Chemical Society最新文献

Fibromyalgia (FM) is a widespread muscle pain disorder that primarily affects females. Osteoarthritis (OA) is another common chronic condition. This study utilized sequential window acquisition of all theoretical mass spectra (SWATH)-based proteomic analysis on the urine of patients with FM and OA. The study identified 54 differentially expressed proteins (DEPs) in the FM group compared to the healthy control (HC) group; 17 DEPs in the OA group compared to the HC group; and 47 DEPs in the FM group compared to the OA group, with a p-value <0.05 and a fold change >1.5 or <0.67. The DEPs in both FM and OA groups were primarily involved in pathways related to coagulation and the complement system, as well as serotonin, dopamine, glutamate pathways, and acute phase reactions. The study suggests that FM and OA induce inflammatory reactions, significantly altering the expression of acute phase proteins and notably decreasing the expression of GNB2. This distinction between health and disease, and the differentiation between FM and OA, is facilitated through the coagulation and complement systems, with SERPING1 and EPCR being key markers. Through these pathways, a better understanding of the impact of FM and OA on the human proteome is achieved.

Extraction of high-quality genomic DNA from wild boar tissue is of great significance for the study of its genetic information, population quantity, and distribution. In this work, a hydrophobic magnetic deep eutectic solvent (HMDES) was synthesized. Based on the HMDES, a HMDES-based vortexed extraction method was developed for extracting genomic DNA from wild boar muscle tissue. Compared to the traditional high salt-extraction method, this approach is faster and embraces higher DNA extraction efficiency. Different pretreatment methods before extraction were evaluated. Single-factor experiments were used to optimize the extraction condition, for example, extraction time, temperature, and HMDES volume. After extraction, the DNA can be quickly and easily recovered from the HMDES phase, and the HMDES can be reused. This work provides a simple and environmental-friendly extraction method for DNA extraction from wild boar tissue.

Fe₃(CO)₁₂ reacted with azadiethylthiols afforded [Fe₂(μ-S(CH₂)₂NR(CH₂)₂S)(CO)₆]₂ (R = ⁿPr, 1A; ⁱPr, 2A). Structure analysis revealed that 1A features the anti-(a,e,a,e) configuration, while its iso-propyl 2A adopts the syn-(a,e,a,e) arrangement. NMR measurements at room temperature confirmed this structure, showing distinct signals for 2 NCH₂ and 2 SCH₂ methylene groups. Acid treatment of 1–2 yielded N-protonated species (1A–H, 2A–H), maintaining the anti-(a,e,a,e) configuration. Isolation of 1B–H revealed both the syn-(a,e,e,a) and anti-(a,e,e,a) configuration by X-ray single-crystallography. Protonation of 1A–H induced downfield shifts in methylene proton signals, with NH hydrogen recorded at 6.96 ppm. Furthermore, 1B–H displays similar NMR behavior, indicating the coexistence of two isomeric molecules of 1B–H, syn-(a,e,e,a) and anti-(a,e,e,a), which is consistent with the crystallographic results. Moreover, ion mobility-mass spectrometry (IM-MS) is harnessed for the swift identification and differentiation of these isomeric complexes in solution. By leveraging accurate mass measurements and MS/MS analyses, the molecular formula and constituents are validated. The sample mobility enables straightforward characterization of 1A, 1B, and 2A in relation to their stereo- and regio-configurations, obviating the need for extensive time or sample quantities. Integration of findings from X-ray crystallography, NMR, and IM-MS furnishes precise and comprehensive structural insights into the coordination complexes.

There have been increasing interests in developing proteomics-based protein adduct detection for monitoring carcinogen exposure risk. Generating adducted protein standards and characterizing the adduction sites by bottom-up proteomics, however, require lengthy adduction and enzymatic digestion. Here we demonstrated that microdroplet can greatly accelerate the catechol estrogen adduction on hemoglobin (Hb) as well as one-pot reaction of adduction and enzyme digestion in millisecond for bottom-up characterization. The adducted Hb generated by microdroplet reaction was characterized to contain one to two catechol estrogens with the β chain of Hb (Hb-β) by online in situ intact measurement of mass spectrometry. The adduction sites were further identified to be C112-Hb-β or C93-Hb-β by microdroplet one-pot or two-step adduction and digestion. These results were consistent with the intact measurement and also same as the bulk or endogenous reaction. This method is expected to be applicable to prepare protein standards adducted by other reactive oxidizing species to greatly save time and sample amount.

In the following research, meso-tetrakis(4-carboxyphenyl)porphyrinatomanganese (III) acetate (MnTCPP(OAc)) was immobilized onto the surface of two functionalized magnetic nanoparticles (MNPs) with different linker length via amide bond (Fe₃O₄@SiO₂-NH₂-MnTCPP(OAc) (A¹) and Fe₃O₄@SiO₂-NHCO-NH₂-MnTCPP(OAc) (A²)). The prepared catalysts were characterized by standard methods such as: atomic absorption spectroscopy (AAS), Ultra violet-visible (UV-Vis), Fourier-transform infrared (FT-IR), vibrating sample magnetometry (VSM), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) techniques and also the effect of the length of the linker on the catalytic activity of supported magnetic nanohybrids was investigated. The prepared magnetic nano-catalysts with different linker lengths were participated in a comparative study of the clean oxidation of sulfides with urea hydrogen peroxide (UHP) in ethanol. The higher catalytic activity, selectivity and also reusability were achieved in the presence of the catalyst with longer linker length (A²) at ambient temperature. Moreover, the turnover number (TON) of catalytic reactions using A² as catalyst was highly enough to consider the catalyst as an efficient material to oxidize various sulfides. Both A¹ and A² catalysts simply have accomplished 5 runs of recycling without significant decrease in the catalytic activity.

In this work, the Tb₂(WO₄)₃/C₃N₄ composite is for the electrochemical sensing of 4-nitrophenol (4-NP). Tb₂(WO₄)₃ was prepared by hydrothermal method. Tb₂(WO₄)₃ homogeneously functionalized with the g-C₃N₄ by a noncovalent π–π interaction. Besides, field emission scanning electron microscopy, x-ray diffraction studies, and energy-dispersive x-ray spectroscopy were utilized to characterize the prepared materials. Cyclic voltammetry and amperometry techniques were used to investigate the electrocatalytic behavior of prepared electrode materials. The reduction in 4-NP exhibits high electrocatalytic activity, with best at working voltage with a linear response range of up to 1200 μM. Moreover, the SPCE/C₃N₄/Tb₂(WO₄)₃ electrode exhibited a detection limit and sensitivity of 0.23 μM and 1.72 μAμM⁻¹ cm⁻², respectively. The electrode provides a workable platform for the analysis of 4-NP in water samples and is highly reproducible and stable.

The early detection and treatment of Helicobacter pylori infections can help prevent the formation of ulcers and development of gastric cancer in humans. The urea breath test (UBT) is a noninvasive test for diagnosing H. pylori. However, isotopic-labeled UBT reagents are expensive and require specialized test equipment to detect isotopic CO₂. In this study, the development of a user-friendly, cost-effective, and alternative method for diagnosing H. pylori infections by detecting NH₃ in exhaled breath is reported. A commercial disposable pH strip is modified with sodium carboxymethylcellulose to enhance its performance in gaseous environments. The modified pH strip exhibits compatibility with both acidic (HCl and CH₃COOH) and alkaline (NH₃) gases. Furthermore, the sensor monitors the production of NH₃ resulting from fish spoilage for 72 h, highlighting its real-time capabilities. The feasibility of employing this simple strip sensor to detect H. pylori infection in patients is investigated through in vitro experiments and preliminary clinical studies. The simple strip sensor is a promising alternative to the C-13 UBT for detecting H. pylori infection.

In this paper, photograph of the sample environ-ment set-up at the TLS 15A1 experimental station of the NSRRC. The storage ring delivers a high-quality light source, and the experimental station is equipped with state-of-the-art instruments. Together, they enable the acquisition of high-resolution complex structures, facili-tating further research and applications. More details about this figure will be discussed by Dr. Chun-Hsiang Huang and his co-worker on page 721–731 in this issue.