首页 > 最新文献

Journal of the Peripheral Nervous System最新文献

英文 中文
Focal slowing of nerve conduction velocity in leprosy patients unveiled through multisegmented nerve analysis 通过多节神经分析揭示麻风病人神经传导速度的灶性减慢。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-08-20 DOI: 10.1111/jns.12649
Vanessa Daccach, Pedro José Tomaselli, Juliana Secchin Algemiro, Patricia Toscano, André Cleriston José dos Santos, Marco Andrey Cipriano Frade, Wilson Marques Jr.

Background and Aims

Leprosy is a chronic infectious disease caused by Mycobacterium leprae (M. leprae), an intracellular bacillus that systematically invades the peripheral nerves. Diagnosing leprosy neuropathy is still a defying skill, and late diagnosis and treatment are still a reality. Based on the biological characteristics of M. leprae, particularly its preference for invading the Schwann cells localized at the coldest areas of human body, we hypothesized that these areas have focal demyelination that may escape detection through standard nerve conduction studies (NCSs) protocols.

Methods

Twenty-five patients with confirmed multibacillary leprosy and 14 controls were accessed. A multisegmented NCS protocol (MP) was performed, targeting short segments through the coldest areas, to identify focal areas of slowed conduction velocity. The effectiveness of this multisegmented protocol was compared to the standard protocol (SP) to detect abnormalities.

Results

All leprosy patients presented an abnormal study with the MP, contrasting to 19 with the SP. The most frequent NCS pattern was an asymmetric neuropathy with focal slowing of conduction velocity, found in 23 out of 25 leprosy patients. Significant differences favoring the proposed method were observed when comparing the MP with the SP. Notably, the MP increased the sensitivity to detect abnormalities by 122%, 133%, and 257% for the median, peroneal, and tibial nerves, respectively. MP also increases sensitivity to detect focal abnormalities in the ulnar nerve.

Interpretation

The MP protocol significantly increases the sensitivity of NCSs to detect neurophysiological abnormalities in leprosy neuropathy.

背景和目的:麻风病是由麻风分枝杆菌(M. leprae)引起的一种慢性传染病,麻风分枝杆菌是一种细胞内杆菌,可系统性地侵犯周围神经。麻风病神经病变的诊断仍是一门高难度技术,晚期诊断和治疗仍是现实。根据麻风杆菌的生物学特性,尤其是其偏好侵袭人体最寒冷部位的许旺细胞,我们推测这些部位存在局灶性脱髓鞘,可能无法通过标准神经传导研究(NCS)方案检测出来:方法:我们访问了 25 名确诊为多疱性麻风病的患者和 14 名对照组患者。采用多分段神经传导检查方案(MP),以通过最冷区域的短节段为目标,识别传导速度减慢的病灶区域。结果显示,所有麻风病人均出现传导速度异常:所有麻风病人在使用 MP 时均出现异常,而使用 SP 时只有 19 人出现异常。最常见的 NCS 模式是不对称神经病变,伴有局灶性传导速度减慢,25 名麻风病人中有 23 人出现这种情况。在将 MP 与 SP 进行比较时,观察到了有利于拟议方法的显著差异。值得注意的是,MP 对正中神经、腓肠神经和胫神经的异常检测灵敏度分别提高了 122%、133% 和 257%。MP 还能提高检测尺神经局灶性异常的灵敏度:MP方案大大提高了NCS检测麻风病神经病变神经电生理异常的灵敏度。
{"title":"Focal slowing of nerve conduction velocity in leprosy patients unveiled through multisegmented nerve analysis","authors":"Vanessa Daccach,&nbsp;Pedro José Tomaselli,&nbsp;Juliana Secchin Algemiro,&nbsp;Patricia Toscano,&nbsp;André Cleriston José dos Santos,&nbsp;Marco Andrey Cipriano Frade,&nbsp;Wilson Marques Jr.","doi":"10.1111/jns.12649","DOIUrl":"10.1111/jns.12649","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Leprosy is a chronic infectious disease caused by <i>Mycobacterium leprae</i> (<i>M. leprae</i>), an intracellular bacillus that systematically invades the peripheral nerves. Diagnosing leprosy neuropathy is still a defying skill, and late diagnosis and treatment are still a reality. Based on the biological characteristics of <i>M. leprae,</i> particularly its preference for invading the Schwann cells localized at the coldest areas of human body, we hypothesized that these areas have focal demyelination that may escape detection through standard nerve conduction studies (NCSs) protocols.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Twenty-five patients with confirmed multibacillary leprosy and 14 controls were accessed. A multisegmented NCS protocol (MP) was performed, targeting short segments through the coldest areas, to identify focal areas of slowed conduction velocity. The effectiveness of this multisegmented protocol was compared to the standard protocol (SP) to detect abnormalities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All leprosy patients presented an abnormal study with the MP, contrasting to 19 with the SP. The most frequent NCS pattern was an asymmetric neuropathy with focal slowing of conduction velocity, found in 23 out of 25 leprosy patients. Significant differences favoring the proposed method were observed when comparing the MP with the SP. Notably, the MP increased the sensitivity to detect abnormalities by 122%, 133%, and 257% for the median, peroneal, and tibial nerves, respectively. MP also increases sensitivity to detect focal abnormalities in the ulnar nerve.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Interpretation</h3>\u0000 \u0000 <p>The MP protocol significantly increases the sensitivity of NCSs to detect neurophysiological abnormalities in leprosy neuropathy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"356-362"},"PeriodicalIF":3.9,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PNS Abstracts 2024 2024 年 PNS 年会 - 加拿大蒙特利尔,2024 年 6 月 22-25 日。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-08-20 DOI: 10.1111/jns.12648
{"title":"PNS Abstracts 2024","authors":"","doi":"10.1111/jns.12648","DOIUrl":"10.1111/jns.12648","url":null,"abstract":"","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 S3","pages":"S3-S193"},"PeriodicalIF":3.9,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term and low-dose rituximab treatment for chronic inflammatory demyelinating polyneuropathy 慢性炎症性脱髓鞘性多发性神经病的长期和低剂量利妥昔单抗治疗。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-08-17 DOI: 10.1111/jns.12653
Yongsheng Zheng, Chong Sun, Yanyin Zhao, Quanhua Meng, Jianian Hu, Kai Qiao, Jian Sun, Jianying Xi, Sushan Luo, Jiahong Lu, Chongbo Zhao, Jie Lin

Objective

To evaluate the efficacy and safety of a low-dose, long-term rituximab regimen in the treatment of idiopathic CIDP.

Methods

This study included 15 CIDP patients treated with rituximab. Patients were administered 600 mg of rituximab intravenously every 6 months. Baseline evaluation was conducted before the initiation of rituximab treatment and subsequent evaluations were conducted 6 months after each rituximab infusion at on-site visits. Clinical improvement was objectively determined by improvement of scale score at least decrease ≥1 INCAT or mRS or increase ≥4 MRC or ≥8 cI-RODS after each infusion compared to baseline evaluation.

Results

Fifteen CIDP patients were included and 10 of them were typical CIDP and five were distal CIDP. Nine in 15 (60%) patients after first infusion and three in six (50%) patients after second infusion exhibited significant clinical improvement compared to baseline evaluation. Additionally, rituximab facilitated a reduction or cessation of other medications in 73% of patients at last visit. The safety profile was favorable, with no reported adverse events.

Conclusion

Rituximab presents a promising therapeutic option for idiopathic CIDP, offering both efficacy and safety with a low-dose, long-term regimen.

目的:评估低剂量、长期利妥昔单抗治疗特发性CIDP的疗效和安全性:评估低剂量、长期利妥昔单抗方案治疗特发性 CIDP 的有效性和安全性:本研究纳入了15名接受利妥昔单抗治疗的CIDP患者。患者每 6 个月静脉注射 600 毫克利妥昔单抗。在开始利妥昔单抗治疗前进行基线评估,每次输注利妥昔单抗 6 个月后进行现场评估。与基线评估相比,每次输注后的量表评分至少下降≥1 INCAT或mRS,或上升≥4 MRC或≥8 cI-RODS,以此客观判定临床改善情况:共纳入15例CIDP患者,其中10例为典型CIDP,5例为远端CIDP。与基线评估结果相比,15 名患者中有 9 人(60%)在第一次输液后临床症状明显改善,6 名患者中有 3 人(50%)在第二次输液后临床症状明显改善。此外,利妥昔单抗还有助于73%的患者在最后一次就诊时减少或停用其他药物。安全性状况良好,无不良反应报告:利妥昔单抗为特发性CIDP提供了一种前景广阔的治疗方案,它以低剂量、长期方案提供了疗效和安全性。
{"title":"Long-term and low-dose rituximab treatment for chronic inflammatory demyelinating polyneuropathy","authors":"Yongsheng Zheng,&nbsp;Chong Sun,&nbsp;Yanyin Zhao,&nbsp;Quanhua Meng,&nbsp;Jianian Hu,&nbsp;Kai Qiao,&nbsp;Jian Sun,&nbsp;Jianying Xi,&nbsp;Sushan Luo,&nbsp;Jiahong Lu,&nbsp;Chongbo Zhao,&nbsp;Jie Lin","doi":"10.1111/jns.12653","DOIUrl":"10.1111/jns.12653","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the efficacy and safety of a low-dose, long-term rituximab regimen in the treatment of idiopathic CIDP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study included 15 CIDP patients treated with rituximab. Patients were administered 600 mg of rituximab intravenously every 6 months. Baseline evaluation was conducted before the initiation of rituximab treatment and subsequent evaluations were conducted 6 months after each rituximab infusion at on-site visits. Clinical improvement was objectively determined by improvement of scale score at least decrease ≥1 INCAT or mRS or increase ≥4 MRC or ≥8 cI-RODS after each infusion compared to baseline evaluation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Fifteen CIDP patients were included and 10 of them were typical CIDP and five were distal CIDP. Nine in 15 (60%) patients after first infusion and three in six (50%) patients after second infusion exhibited significant clinical improvement compared to baseline evaluation. Additionally, rituximab facilitated a reduction or cessation of other medications in 73% of patients at last visit. The safety profile was favorable, with no reported adverse events.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Rituximab presents a promising therapeutic option for idiopathic CIDP, offering both efficacy and safety with a low-dose, long-term regimen.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"350-355"},"PeriodicalIF":3.9,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Earlier diagnosis of peripheral neuropathy in primary care: A call to action” 更正 "基层医疗机构更早地诊断周围神经病变:行动呼吁"。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-08-14 DOI: 10.1111/jns.12652

Gad H, Kalra S, Pinzon R, et al. Earlier diagnosis of peripheral neuropathy in primary care: a call to action. J Peripher Nerv Syst. 2024;29(1):28-37. doi:10.1111/jns.12613

It has come to our attention that there was an error in the spelling of one of the co-author's names in the above article. The correct spelling should be Rey-an Nino Garcia instead of Rey-an Nino Gracia. The article has been corrected.

We apologize for this error.

Gad H、Kalra S、Pinzon R 等人.初级保健中周围神经病变的早期诊断:行动呼吁。J Peripher Nerv Syst.2024;29(1):28-37.DOI:10.1111/jns.12613我们注意到,上述文章中一位合著者的姓名拼写有误。正确的拼写应该是 Rey-an Nino Garcia,而不是 Rey-an Nino Gracia。我们对此错误深表歉意。
{"title":"Correction to “Earlier diagnosis of peripheral neuropathy in primary care: A call to action”","authors":"","doi":"10.1111/jns.12652","DOIUrl":"10.1111/jns.12652","url":null,"abstract":"<p>Gad H, Kalra S, Pinzon R, et al. Earlier diagnosis of peripheral neuropathy in primary care: a call to action. <i>J Peripher Nerv Syst</i>. 2024;29(1):28-37. doi:10.1111/jns.12613</p><p>It has come to our attention that there was an error in the spelling of one of the co-author's names in the above article. The correct spelling should be Rey-an Nino Garcia instead of Rey-an Nino Gracia. The article has been corrected.</p><p>We apologize for this error.</p>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"378"},"PeriodicalIF":3.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jns.12652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic and clinical utility of comprehensive multigene panel testing for patients with neuropathy 神经病变患者多基因综合面板检测的诊断和临床实用性。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-08-14 DOI: 10.1111/jns.12651
Jennifer Roggenbuck, Ana Morales, Colin A. Ellis, Laynie Dratch, Molly Stetler, Christopher A. Tan, Brianna Bucknor, Kathryn E. Hatchell, Swaroop Aradhya, Edward D. Esplin, Yi-Lee Ting, Steven S. Scherer

Background and Aims

Prior to next-generation sequencing (NGS), the evaluation of a patient with neuropathy typically consisted of screening for acquired causes, followed by clinical genetic testing of PMP22, MFN2, GJB1, and MPZ in patients with a positive family history and symptom onset prior to age 50. In this study, we examined the clinical utility of NGS in a large cohort of patients analyzed in a commercial laboratory.

Methods

A cohort of 6849 adult patients underwent clinician-ordered peripheral neuropathy multigene panel testing ranging from 66 to 111 genes that included NGS and intragenic deletion/duplication analysis.

Results

A molecular diagnosis was identified for 8.4% of the cohort (n = 573/6849). Variants in PMP22, MFN2, GJB1, MPZ, and TTR accounted for 73.8% of molecular diagnoses. Results had potential clinical actionability for 398 (69.5%) patients. Our results suggest that 225/573 (39.3%) of molecular diagnoses and 113/398 (28.4%) of clinical interventions would have been missed if the testing approach had been restricted to older guidelines.

Interpretation

Our results highlight the need for expanded genetic testing guidelines that account for the increased number of genes associated with hereditary neuropathy, address the overlap of acquired and hereditary neuropathy, and provide broader access to genetic diagnosis for patients.

背景和目的:在使用新一代测序技术(NGS)之前,对神经病患者的评估通常包括筛查获得性病因,然后对有阳性家族史且在 50 岁之前发病的患者进行 PMP22、MFN2、GJB1 和 MPZ 的临床基因检测。在本研究中,我们在商业实验室分析的一大批患者中考察了 NGS 的临床实用性:6849 名成年患者接受了临床医生要求的周围神经病变多基因组检测,检测范围从 66 个基因到 111 个基因,包括 NGS 和基因内缺失/重复分析:结果:8.4%的患者(n = 573/6849)得到了分子诊断。PMP22、MFN2、GJB1、MPZ 和 TTR 的变异占分子诊断的 73.8%。398例(69.5%)患者的结果具有潜在的临床可操作性。我们的结果表明,如果检测方法仅限于旧版指南,将错过 225/573 例(39.3%)分子诊断和 113/398 例(28.4%)临床干预:我们的研究结果凸显了扩大基因检测指南范围的必要性,这些指南应考虑到与遗传性神经病相关基因数量的增加,解决获得性和遗传性神经病的重叠问题,并为患者提供更广泛的基因诊断途径。
{"title":"Diagnostic and clinical utility of comprehensive multigene panel testing for patients with neuropathy","authors":"Jennifer Roggenbuck,&nbsp;Ana Morales,&nbsp;Colin A. Ellis,&nbsp;Laynie Dratch,&nbsp;Molly Stetler,&nbsp;Christopher A. Tan,&nbsp;Brianna Bucknor,&nbsp;Kathryn E. Hatchell,&nbsp;Swaroop Aradhya,&nbsp;Edward D. Esplin,&nbsp;Yi-Lee Ting,&nbsp;Steven S. Scherer","doi":"10.1111/jns.12651","DOIUrl":"10.1111/jns.12651","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Prior to next-generation sequencing (NGS), the evaluation of a patient with neuropathy typically consisted of screening for acquired causes, followed by clinical genetic testing of <i>PMP22</i>, <i>MFN2</i>, <i>GJB1</i>, and <i>MPZ</i> in patients with a positive family history and symptom onset prior to age 50. In this study, we examined the clinical utility of NGS in a large cohort of patients analyzed in a commercial laboratory.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A cohort of 6849 adult patients underwent clinician-ordered peripheral neuropathy multigene panel testing ranging from 66 to 111 genes that included NGS and intragenic deletion/duplication analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A molecular diagnosis was identified for 8.4% of the cohort (<i>n</i> = 573/6849). Variants in <i>PMP22</i>, <i>MFN2</i>, <i>GJB1</i>, <i>MPZ</i>, and <i>TTR</i> accounted for 73.8% of molecular diagnoses. Results had potential clinical actionability for 398 (69.5%) patients. Our results suggest that 225/573 (39.3%) of molecular diagnoses and 113/398 (28.4%) of clinical interventions would have been missed if the testing approach had been restricted to older guidelines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Interpretation</h3>\u0000 \u0000 <p>Our results highlight the need for expanded genetic testing guidelines that account for the increased number of genes associated with hereditary neuropathy, address the overlap of acquired and hereditary neuropathy, and provide broader access to genetic diagnosis for patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"363-367"},"PeriodicalIF":3.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jns.12651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Initial findings using high-resolution magnetic resonance imaging for visualisation of the sural nerve and surrounding anatomy in healthy volunteers at 7 Tesla 使用高分辨率磁共振成像在 7 特斯拉下观察健康志愿者的硬脊膜神经和周围解剖结构的初步发现。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-07-26 DOI: 10.1111/jns.12645
Amy R. McDowell, Laura Zambreanu, Hamza A. Salhab, Carolynne M. Doherty, Philippa Bridgen, Pete Lally, Sachit Shah, Zimu Huo, Stephen J. Wastling, Tarek Yousry, Jasper Morrow, John S. Thornton, Michael P. Lunn

Background and Aims

Histopathological diagnosis is the gold standard in many acquired inflammatory, infiltrative and amyloid based peripheral nerve diseases and a sensory nerve biopsy of sural or superficial peroneal nerve is favoured where a biopsy is deemed necessary. The ability to determine nerve pathology by high-resolution imaging techniques resolving anatomy and imaging characteristics might improve diagnosis and obviate the need for biopsy in some. The sural nerve is anatomically variable and occasionally adjacent vessels can be sent for analysis in error. Knowing the exact position and relationships of the nerve prior to surgery could be clinically useful and thus reliably resolving nerve position has some utility.

Methods

7T images of eight healthy volunteers' (HV) right ankle were acquired in a pilot study using a double-echo in steady-state sequence for high-resolution anatomy images. Magnetic Transfer Ratio images were acquired of the same area. Systematic scoring of the sural, tibial and deep peroneal nerve around the surgical landmark 7 cm from the lateral malleolus was performed (number of fascicles, area in voxels and mm2, diameter and location relative to nearby vessels and muscles).

Results

The sural and tibial nerves were visualised in the high-resolution double-echo in steady-state (DESS) image in all HV. The deep peroneal nerve was not always visualised at level of interest. The MTR values were tightly grouped except in the sural nerve where the nerve was not visualised in two HV. The sural nerve location was found to be variable (e.g., lateral or medial to, or crossing behind, or found positioned directly posterior to the saphenous vein).

Interpretation

High-resolution high-field images have excellent visualisation of the sural nerve and would give surgeons prior knowledge of the position before surgery. Basic imaging characteristics of the sural nerve can be acquired, but more detailed imaging characteristics are not easily evaluable in the very small sural and further developments and specific studies are required for any diagnostic utility at 7T.

背景和目的:组织病理学诊断是许多获得性炎症、浸润性和淀粉样变性周围神经疾病的金标准,在认为有必要进行活检的情况下,应首选对硬神经或腓浅神经进行感觉神经活检。通过高分辨率成像技术来确定神经病变的解剖和成像特征可能会改善诊断,并使某些患者无需进行活检。鞍神经在解剖学上是多变的,偶尔会错误地将邻近的血管送去分析。手术前了解神经的确切位置和关系可能对临床有用,因此可靠地确定神经位置具有一定的实用性。方法:在一项试验性研究中,使用稳态双回波序列获取了八名健康志愿者(HV)右踝关节的 7T 图像,以获得高分辨率解剖图像。同时还采集了同一区域的磁转移比图像。在距外侧踝骨 7 厘米的手术标志周围对硬神经、胫神经和腓深神经进行了系统评分(筋束数量、体素和平方毫米面积、直径以及与附近血管和肌肉的相对位置):在所有 HV 的高分辨率稳态双回波(DESS)图像中都能看到鞍神经和胫神经。腓深神经并不总能在感兴趣水平上显示出来。除了在两个 HV 中未观察到硬脊膜神经外,其他 MTR 值都是紧密分组的。腓总神经的位置可变(例如,位于隐静脉的外侧或内侧,或穿过隐静脉的后方,或位于隐静脉的正后方):解读:高分辨率的高场图像可清晰显示腓肠神经,使外科医生在手术前就能预先了解其位置。可以获得腓肠神经的基本成像特征,但在非常小的腓肠神经中不容易评估更详细的成像特征,需要进一步开发和具体研究才能在 7T 下进行诊断。
{"title":"Initial findings using high-resolution magnetic resonance imaging for visualisation of the sural nerve and surrounding anatomy in healthy volunteers at 7 Tesla","authors":"Amy R. McDowell,&nbsp;Laura Zambreanu,&nbsp;Hamza A. Salhab,&nbsp;Carolynne M. Doherty,&nbsp;Philippa Bridgen,&nbsp;Pete Lally,&nbsp;Sachit Shah,&nbsp;Zimu Huo,&nbsp;Stephen J. Wastling,&nbsp;Tarek Yousry,&nbsp;Jasper Morrow,&nbsp;John S. Thornton,&nbsp;Michael P. Lunn","doi":"10.1111/jns.12645","DOIUrl":"10.1111/jns.12645","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Histopathological diagnosis is the gold standard in many acquired inflammatory, infiltrative and amyloid based peripheral nerve diseases and a sensory nerve biopsy of sural or superficial peroneal nerve is favoured where a biopsy is deemed necessary. The ability to determine nerve pathology by high-resolution imaging techniques resolving anatomy and imaging characteristics might improve diagnosis and obviate the need for biopsy in some. The sural nerve is anatomically variable and occasionally adjacent vessels can be sent for analysis in error. Knowing the exact position and relationships of the nerve prior to surgery could be clinically useful and thus reliably resolving nerve position has some utility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>7T images of eight healthy volunteers' (HV) right ankle were acquired in a pilot study using a double-echo in steady-state sequence for high-resolution anatomy images. Magnetic Transfer Ratio images were acquired of the same area. Systematic scoring of the sural, tibial and deep peroneal nerve around the surgical landmark 7 cm from the lateral malleolus was performed (number of fascicles, area in voxels and mm<sup>2</sup>, diameter and location relative to nearby vessels and muscles).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The sural and tibial nerves were visualised in the high-resolution double-echo in steady-state (DESS) image in all HV. The deep peroneal nerve was not always visualised at level of interest. The MTR values were tightly grouped except in the sural nerve where the nerve was not visualised in two HV. The sural nerve location was found to be variable (e.g., lateral or medial to, or crossing behind, or found positioned directly posterior to the saphenous vein).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Interpretation</h3>\u0000 \u0000 <p>High-resolution high-field images have excellent visualisation of the sural nerve and would give surgeons prior knowledge of the position before surgery. Basic imaging characteristics of the sural nerve can be acquired, but more detailed imaging characteristics are not easily evaluable in the very small sural and further developments and specific studies are required for any diagnostic utility at 7T.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"368-375"},"PeriodicalIF":3.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jns.12645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to ‘Serum neurofilament light chain measurements following nerve trauma’ 神经创伤后血清神经丝轻链的测量结果 "的更正。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-07-11 DOI: 10.1111/jns.12642

Wilcox M, Rayner MLD, Guillemot-Legris O, Platt I, Brown H, Quick T, Phillips JB Serum neurofilament light chain measurements following nerve trauma. J Peripher Nerv Syst. 2023;28(3):500–507. doi:10.1111/jns.12576

There was an error in the analysis of serum NfL data that resulted in the values in Figure 1, Table 1 and Results being expressed incorrectly as 40-fold lower than their correct concentration. The ‘Serum NfL analysis’ section of the Methods should read ‘Serum NfL concentration was measured using the Simoa HD-X Analyser (Quanterix, Billerica, MA) with a Neurology 4-plex B kit (item number: 103345). All samples were randomised across the plates and run in duplicate. All samples measured were above the lower limit of detection (0.0962 pg/mL) with a mean coefficient of variation of 13.2% between duplicates. Intra- and inter-plate coefficients of variation, monitored using 2-4 pooled human internal control samples across each plate, were <10%’. not ‘NfL levels in serum were measured using a SIMOA® SR-X analyser (Quanterix Corporation, Massachusetts) with a SIMOA® NF-light Advantage (SR-X) Kit (Quanterix, No. 103400) according to protocols well described elsewhere (O'Brien, et al., 2021). All samples were run in duplicate and analysers were blinded to the experimental conditions. All samples measured above the lower limit of detection (0.038 pg/mL) with a mean coefficient of variation of 9.15% between duplicates. Furthermore, two control samples were run on each place with a mean coefficient of variation of 6.90%’.

New versions of Figure 1 and Table 1 have been provided. In addition, the second line of the Results section should read 20.33 ± 8.98 pg/mL rather than 0.50 ± 0.2 pg/mL. The third line of the Discussion section should read 20 pg/mL rather than 0.50 pg/mL.

We apologise for this error.

Wilcox M、Rayner MLD、Guillemot-Legris O、Platt I、Brown H、Quick T、Phillips JB 神经创伤后的血清神经丝轻链测量。J Peripher Nerv Syst.doi:10.1111/jns.12576在分析血清神经丝轻链数据时出现错误,导致图 1、表 1 和结果中的数值被错误地表示为比其正确浓度低 40 倍。方法》中的 "血清 NfL 分析 "部分应为 "使用 Simoa HD-X 分析仪(Quanterix, Billerica, MA)和神经学 4-plex B 试剂盒(货号:103345)测量血清 NfL 浓度。所有样品在平板上随机排列,一式两份。所有测定的样品均高于检测下限(0.0962 pg/mL),重复样品之间的平均变异系数为 13.2%。使用每个板上的 2-4 个集合人类内部对照样本监测板内和板间变异系数,结果均为 <10%'。而非'使用 SIMOA® SR-X 分析仪(Quanterix Corporation,马萨诸塞州)和 SIMOA® NF-light Advantage (SR-X) 试剂盒(Quanterix,编号 103400),按照其他地方详细描述的方案(O'Brien 等人,2021 年)测量血清中的 NfL 水平。所有样品均一式两份,分析人员对实验条件保密。所有样本的测量值均高于检测下限(0.038 pg/mL),重复样本之间的平均变异系数为 9.15%。图 1 和表 1 提供了新版本。此外,结果部分第二行应为 20.33 ± 8.98 pg/mL,而不是 0.50 ± 0.2 pg/mL。讨论部分第三行应为 20 pg/mL,而不是 0.50 pg/mL。
{"title":"Correction to ‘Serum neurofilament light chain measurements following nerve trauma’","authors":"","doi":"10.1111/jns.12642","DOIUrl":"10.1111/jns.12642","url":null,"abstract":"<p>Wilcox M, Rayner MLD, Guillemot-Legris O, Platt I, Brown H, Quick T, Phillips JB Serum neurofilament light chain measurements following nerve trauma. J Peripher Nerv Syst. 2023;28(3):500–507. doi:10.1111/jns.12576</p><p>There was an error in the analysis of serum NfL data that resulted in the values in Figure 1, Table 1 and Results being expressed incorrectly as 40-fold lower than their correct concentration. The ‘Serum NfL analysis’ section of the Methods should read ‘Serum NfL concentration was measured using the Simoa HD-X Analyser (Quanterix, Billerica, MA) with a Neurology 4-plex B kit (item number: 103345). All samples were randomised across the plates and run in duplicate. All samples measured were above the lower limit of detection (0.0962 pg/mL) with a mean coefficient of variation of 13.2% between duplicates. Intra- and inter-plate coefficients of variation, monitored using 2-4 pooled human internal control samples across each plate, were &lt;10%’. not ‘NfL levels in serum were measured using a SIMOA® SR-X analyser (Quanterix Corporation, Massachusetts) with a SIMOA® NF-light Advantage (SR-X) Kit (Quanterix, No. 103400) according to protocols well described elsewhere (O'Brien, et al., 2021). All samples were run in duplicate and analysers were blinded to the experimental conditions. All samples measured above the lower limit of detection (0.038 pg/mL) with a mean coefficient of variation of 9.15% between duplicates. Furthermore, two control samples were run on each place with a mean coefficient of variation of 6.90%’.</p><p>New versions of Figure 1 and Table 1 have been provided. In addition, the second line of the Results section should read 20.33 ± 8.98 pg/mL rather than 0.50 ± 0.2 pg/mL. The third line of the Discussion section should read 20 pg/mL rather than 0.50 pg/mL.</p><p>We apologise for this error.</p>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"376-377"},"PeriodicalIF":3.9,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jns.12642","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy and safety of eculizumab in Guillain-Barré syndrome: A phase 3, multicenter, double-blind, randomized, placebo-controlled clinical trial 依库珠单抗治疗格林-巴利综合征的有效性和安全性:3期多中心、双盲、随机、安慰剂对照临床试验。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-07-10 DOI: 10.1111/jns.12646
Satoshi Kuwabara, Susumu Kusunoki, Motoi Kuwahara, Yoshihisa Yamano, Yoichiro Nishida, Hirokazu Ishida, Tomoyuki Kasuya, Erik Kupperman, Qun Lin, Glen Frick, Sonoko Misawa

Background and Aims

Guillain-Barré syndrome (GBS) is an acute, self-limited, immune-mediated peripheral neuropathy. Current treatments for GBS include intravenous immunoglobulin (IVIg) and plasma exchange, which may not sufficiently benefit severely affected patients. This study evaluated the efficacy and safety of eculizumab add-on therapy to IVIg (standard-of-care treatment) in patients with severe GBS.

Methods

This phase 3, multicenter, double-blind, randomized, placebo-controlled clinical trial (NCT04752566), enrolled Japanese adults (age ≥ 18 years) with severe GBS (Hughes functional grade [FG] score FG3 or FG4/FG5 within 2 weeks of onset of GBS). Participants were randomized 2:1 to receive intravenous infusion of eculizumab or placebo (once weekly for 4 weeks) with IVIg treatment with 20 weeks of follow-up. Primary efficacy endpoint was the time to first reach FG score ≤1 (able to run). Key secondary endpoints were proportion of participants achieving FG ≤1 at weeks 8 and 24 and FG improvement ≥3 at week 24. Pharmacodynamic analysis of serum free C5 concentration over time was performed. Safety was evaluated.

Results

The analysis included 57 participants (eculizumab, n = 37; placebo, n = 20). Primary endpoint was not achieved (hazard ratio, 0.9; 95% CI, 0.45–1.97; p = .89). Key secondary endpoints did not reach statistical significance. Serum C5 concentration was reduced by 99.99% at 1 h postdose and sustained to week 5 but returned to baseline at the end of follow-up period. No new safety signals for eculizumab were identified.

Interpretation

Although well tolerated, eculizumab treatment did not show significant effects on motor function recovery compared to placebo in patients with GBS.

背景和目的:吉兰-巴雷综合征(GBS)是一种急性、自限性、免疫介导的周围神经病。目前治疗吉兰-巴雷综合征的方法包括静脉注射免疫球蛋白(IVIg)和血浆置换,但这两种方法可能无法使重症患者充分受益。本研究评估了依库珠单抗作为IVIg(标准疗法)的附加疗法对重症GBS患者的疗效和安全性:这项 3 期、多中心、双盲、随机、安慰剂对照临床试验(NCT04752566)招募了患有重症 GBS(GBS 发病 2 周内休斯功能分级 [FG] 评分 FG3 或 FG4/FG5)的日本成人(年龄≥ 18 岁)。参与者按 2:1 随机分配接受静脉输注 eculizumab 或安慰剂(每周一次,共 4 周),并接受 20 周的 IVIg 治疗。主要疗效终点是首次达到FG评分≤1(能跑步)的时间。主要次要终点是第8周和第24周达到FG≤1的参与者比例,以及第24周FG改善≥3。对血清游离 C5 浓度随时间变化进行药效学分析。对安全性进行了评估:分析包括57名参与者(依库珠单抗,n = 37;安慰剂,n = 20)。主要终点未达到(危险比,0.9;95% CI,0.45-1.97;P = .89)。主要次要终点未达到统计学意义。用药后1小时血清C5浓度降低了99.99%,并持续到第5周,但在随访期结束时又恢复到基线水平。未发现依库珠单抗有新的安全性信号:尽管耐受性良好,但与安慰剂相比,依库珠单抗治疗对GBS患者运动功能的恢复没有显著影响。
{"title":"Efficacy and safety of eculizumab in Guillain-Barré syndrome: A phase 3, multicenter, double-blind, randomized, placebo-controlled clinical trial","authors":"Satoshi Kuwabara,&nbsp;Susumu Kusunoki,&nbsp;Motoi Kuwahara,&nbsp;Yoshihisa Yamano,&nbsp;Yoichiro Nishida,&nbsp;Hirokazu Ishida,&nbsp;Tomoyuki Kasuya,&nbsp;Erik Kupperman,&nbsp;Qun Lin,&nbsp;Glen Frick,&nbsp;Sonoko Misawa","doi":"10.1111/jns.12646","DOIUrl":"10.1111/jns.12646","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Guillain-Barré syndrome (GBS) is an acute, self-limited, immune-mediated peripheral neuropathy. Current treatments for GBS include intravenous immunoglobulin (IVIg) and plasma exchange, which may not sufficiently benefit severely affected patients. This study evaluated the efficacy and safety of eculizumab add-on therapy to IVIg (standard-of-care treatment) in patients with severe GBS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This phase 3, multicenter, double-blind, randomized, placebo-controlled clinical trial (NCT04752566), enrolled Japanese adults (age ≥ 18 years) with severe GBS (Hughes functional grade [FG] score FG3 or FG4/FG5 within 2 weeks of onset of GBS). Participants were randomized 2:1 to receive intravenous infusion of eculizumab or placebo (once weekly for 4 weeks) with IVIg treatment with 20 weeks of follow-up. Primary efficacy endpoint was the time to first reach FG score ≤1 (able to run). Key secondary endpoints were proportion of participants achieving FG ≤1 at weeks 8 and 24 and FG improvement ≥3 at week 24. Pharmacodynamic analysis of serum free C5 concentration over time was performed. Safety was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis included 57 participants (eculizumab, <i>n</i> = 37; placebo, <i>n</i> = 20). Primary endpoint was not achieved (hazard ratio, 0.9; 95% CI, 0.45–1.97; <i>p</i> = .89). Key secondary endpoints did not reach statistical significance. Serum C5 concentration was reduced by 99.99% at 1 h postdose and sustained to week 5 but returned to baseline at the end of follow-up period. No new safety signals for eculizumab were identified.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Interpretation</h3>\u0000 \u0000 <p>Although well tolerated, eculizumab treatment did not show significant effects on motor function recovery compared to placebo in patients with GBS.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"339-349"},"PeriodicalIF":3.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jns.12646","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical, histologic, and immunologic signatures of Small Fiber Neuropathy in Systemic Lupus Erythematosus 系统性红斑狼疮小纤维神经病的临床、组织学和免疫学特征。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-07-09 DOI: 10.1111/jns.12644
Eleonora Galosi, Carmelo Pirone, Fulvia Ceccarelli, Nicoletta Esposito, Pietro Falco, Martina Leopizzi, Valeria Di Maio, Lorenzo Tramontana, Gianfranco De Stefano, Giuseppe Di Pietro, Giulia Di Stefano, Cristina Garufi, Caterina Leone, Francesco Natalucci, Valeria Orefice, Cristiano Alessandri, Francesca Romana Spinelli, Andrea Truini, Fabrizio Conti

Background and Objectives

Systemic Lupus Erythematosus (SLE) often causes damage to small nerve fibers, leading to distressing painful and autonomic symptoms. Despite this, Small Fiber Neuropathy (SFN) remains an underrecognized complication for SLE patients. In this cross-sectional study, we aimed to assess SFN in patients with SLE and to explore its correlations with immunologic disease features and clinical manifestations.

Methods

We recruited 50 SLE patients (1 male to 12.5 females, aged 20–80 years) reporting painful disturbances. We conducted a comprehensive clinical and neurophysiological evaluation, using Nerve Conduction Studies and Quantitative Sensory Testing. Additionally, we carried out an extensive laboratory assessment of disease-related serological parameters. We also performed a thorough skin biopsy analysis, investigating somatic and autonomic innervation while detecting complement and inflammatory cell infiltrates within the skin.

Results

Out of 50 patients, 19 were diagnosed with SFN, primarily characterized by a non-length-dependent distribution; 7 had a mixed neuropathy, with both large and small fiber involvement. Patients with SFN were younger than patients with a mixed neuropathy (p = .0143); furthermore, they were more likely to have a history of hypocomplementemia (p = .0058) and to be treated with cyclosporine A (p = .0053) compared to patients without neuropathy. However, there were no significant differences in painful and autonomic symptoms between patients with and without SFN.

Discussion

This study highlights the relevant frequency of SFN with a non-length-dependent distribution among SLE patients experiencing painful symptoms. Indeed, SFN emerges as an early manifestation of SLE-related neuropathy and is closely associated with hypocomplementemia, suggesting a potential pathogenic role of the complement system. Moreover, SFN may be influenced by disease-modifying therapies. However, the precise role of SFN in shaping painful and autonomic symptoms in patients with SLE remains to be fully elucidated.

背景和目的:系统性红斑狼疮(SLE)常常会对小神经纤维造成损伤,导致令人痛苦的疼痛和自主神经症状。尽管如此,小神经纤维病变(SFN)仍然是系统性红斑狼疮患者未得到充分认识的并发症。在这项横断面研究中,我们旨在评估系统性红斑狼疮患者的小纤维神经病变,并探讨其与免疫学疾病特征和临床表现的相关性:方法:我们招募了50名报告有疼痛障碍的系统性红斑狼疮患者(男性1人,女性12.5人,年龄20-80岁)。我们使用神经传导研究和定量感觉测试对患者进行了全面的临床和神经生理学评估。此外,我们还对与疾病相关的血清学参数进行了广泛的实验室评估。我们还进行了全面的皮肤活检分析,研究躯体和自主神经支配,同时检测皮肤内的补体和炎性细胞浸润:在 50 名患者中,19 人被确诊为 SFN,其主要特征是非长度依赖性分布;7 人患有混合型神经病,大纤维和小纤维均受累。与混合型神经病变患者相比,SFN 患者更年轻(p = .0143);此外,与无神经病变患者相比,SFN 患者更可能有低补体血症病史(p = .0058),也更可能接受环孢素 A 治疗(p = .0053)。但是,有SFN和没有SFN的患者在疼痛和自主神经症状方面没有明显差异:讨论:本研究强调了在出现疼痛症状的系统性红斑狼疮患者中,SFN的发生率与长度无关。事实上,SFN 是系统性红斑狼疮相关神经病变的早期表现,与低补体血症密切相关,这表明补体系统具有潜在的致病作用。此外,SFN 还可能受到疾病改变疗法的影响。然而,SFN 在形成系统性红斑狼疮患者疼痛和自主神经症状方面的确切作用仍有待全面阐明。
{"title":"Clinical, histologic, and immunologic signatures of Small Fiber Neuropathy in Systemic Lupus Erythematosus","authors":"Eleonora Galosi,&nbsp;Carmelo Pirone,&nbsp;Fulvia Ceccarelli,&nbsp;Nicoletta Esposito,&nbsp;Pietro Falco,&nbsp;Martina Leopizzi,&nbsp;Valeria Di Maio,&nbsp;Lorenzo Tramontana,&nbsp;Gianfranco De Stefano,&nbsp;Giuseppe Di Pietro,&nbsp;Giulia Di Stefano,&nbsp;Cristina Garufi,&nbsp;Caterina Leone,&nbsp;Francesco Natalucci,&nbsp;Valeria Orefice,&nbsp;Cristiano Alessandri,&nbsp;Francesca Romana Spinelli,&nbsp;Andrea Truini,&nbsp;Fabrizio Conti","doi":"10.1111/jns.12644","DOIUrl":"10.1111/jns.12644","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Systemic Lupus Erythematosus (SLE) often causes damage to small nerve fibers, leading to distressing painful and autonomic symptoms. Despite this, Small Fiber Neuropathy (SFN) remains an underrecognized complication for SLE patients. In this cross-sectional study, we aimed to assess SFN in patients with SLE and to explore its correlations with immunologic disease features and clinical manifestations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We recruited 50 SLE patients (1 male to 12.5 females, aged 20–80 years) reporting painful disturbances. We conducted a comprehensive clinical and neurophysiological evaluation, using Nerve Conduction Studies and Quantitative Sensory Testing. Additionally, we carried out an extensive laboratory assessment of disease-related serological parameters. We also performed a thorough skin biopsy analysis, investigating somatic and autonomic innervation while detecting complement and inflammatory cell infiltrates within the skin.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Out of 50 patients, 19 were diagnosed with SFN, primarily characterized by a non-length-dependent distribution; 7 had a mixed neuropathy, with both large and small fiber involvement. Patients with SFN were younger than patients with a mixed neuropathy (<i>p</i> = .0143); furthermore, they were more likely to have a history of hypocomplementemia (<i>p</i> = .0058) and to be treated with cyclosporine A (<i>p</i> = .0053) compared to patients without neuropathy. However, there were no significant differences in painful and autonomic symptoms between patients with and without SFN.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>This study highlights the relevant frequency of SFN with a non-length-dependent distribution among SLE patients experiencing painful symptoms. Indeed, SFN emerges as an early manifestation of SLE-related neuropathy and is closely associated with hypocomplementemia, suggesting a potential pathogenic role of the complement system. Moreover, SFN may be influenced by disease-modifying therapies. However, the precise role of SFN in shaping painful and autonomic symptoms in patients with SLE remains to be fully elucidated.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"315-328"},"PeriodicalIF":3.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of cellular and noncellular components of mature intact human peripheral nerve 鉴定成熟完整人类周围神经的细胞和非细胞成分。
IF 3.9 3区 医学 Q1 CLINICAL NEUROLOGY Pub Date : 2024-07-07 DOI: 10.1111/jns.12643
Gabriela I. Aparicio, Jorge E. Quintero, Lauren Plum, Lingxiao Deng, Kristen Wanczyk, Miriam Henry, Evan Lynch, Michael Murphy, Greg A. Gerhardt, Craig G. van Horne, Paula V. Monje

Background and Aims

The goal of this study was to define basic constituents of the adult peripheral nervous system (PNS) using intact human nerve tissues.

Methods

We combined fluorescent and chromogenic immunostaining methods, myelin-selective fluorophores, and routine histological stains to identify common cellular and noncellular elements in aldehyde-fixed nerve tissue sections. We employed Schwann cell (SC)-specific markers, such as S100β, NGFR, Sox10, and myelin protein zero (MPZ), together with axonal, extracellular matrix (collagen IV, laminin, fibronectin), and fibroblast markers to assess the SC's relationship to myelin sheaths, axons, other cell types, and the acellular environment.

Results

Whereas S100β and Sox10 revealed mature SCs in the absence of other stains, discrimination between myelinating and non-myelinating (Remak) SCs required immunodetection of NGFR along with axonal and/or myelin markers. Surprisingly, our analysis of NGFR+ profiles uncovered the existence of at least 3 different novel populations of NGFR+/S100β− cells, herein referred to as nonglial cells, residing in the stroma and perivascular areas of all nerve compartments. An important proportion of the nerve's cellular content, including circa 30% of endoneurial cells, consisted of heterogenous S100β negative cells that were not associated with axons. Useful markers to identify the localization and diversity of nonglial cell types across different compartments were Thy1, CD34, SMA, and Glut1, a perineurial cell marker.

Interpretation

Our optimized methods revealed additional detailed information to update our understanding of the complexity and spatial orientation of PNS-resident cell types in humans.

背景和目的本研究的目的是利用完整的人类神经组织确定成人周围神经系统(PNS)的基本成分:我们结合了荧光和色原免疫染色法、髓鞘选择性荧光团和常规组织学染色法,以确定醛固定神经组织切片中常见的细胞和非细胞元素。我们采用许旺细胞(SC)特异性标记物,如S100β、NGFR、Sox10和髓鞘蛋白零(MPZ),以及轴突、细胞外基质(胶原蛋白IV、层粘连蛋白、纤维连接蛋白)和成纤维细胞标记物来评估SC与髓鞘、轴突、其他细胞类型和无细胞环境的关系:结果:在没有其他染色剂的情况下,S100β和Sox10能显示成熟的SC,而区分髓鞘化和非髓化(Remak)SC则需要免疫检测NGFR以及轴突和/或髓鞘标记物。令人惊奇的是,我们对 NGFR+ 图谱的分析发现,至少存在 3 种不同的新型 NGFR+/S100β- 细胞群,在此称为非神经细胞,它们驻留在所有神经区的基质和血管周围区域。神经细胞的重要组成部分,包括约30%的内膜细胞,由与轴突无关的异源S100β阴性细胞组成。Thy1、CD34、SMA和Glut1(神经周围细胞标志物)是识别不同区段非神经胶质细胞类型定位和多样性的有用标志物:我们的优化方法揭示了更多详细信息,更新了我们对人类 PNS 驻留细胞类型的复杂性和空间定位的认识。
{"title":"Identification of cellular and noncellular components of mature intact human peripheral nerve","authors":"Gabriela I. Aparicio,&nbsp;Jorge E. Quintero,&nbsp;Lauren Plum,&nbsp;Lingxiao Deng,&nbsp;Kristen Wanczyk,&nbsp;Miriam Henry,&nbsp;Evan Lynch,&nbsp;Michael Murphy,&nbsp;Greg A. Gerhardt,&nbsp;Craig G. van Horne,&nbsp;Paula V. Monje","doi":"10.1111/jns.12643","DOIUrl":"10.1111/jns.12643","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>The goal of this study was to define basic constituents of the adult peripheral nervous system (PNS) using intact human nerve tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We combined fluorescent and chromogenic immunostaining methods, myelin-selective fluorophores, and routine histological stains to identify common cellular and noncellular elements in aldehyde-fixed nerve tissue sections. We employed Schwann cell (SC)-specific markers, such as S100β, NGFR, Sox10, and myelin protein zero (MPZ), together with axonal, extracellular matrix (collagen IV, laminin, fibronectin), and fibroblast markers to assess the SC's relationship to myelin sheaths, axons, other cell types, and the acellular environment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Whereas S100β and Sox10 revealed mature SCs in the absence of other stains, discrimination between myelinating and non-myelinating (Remak) SCs required immunodetection of NGFR along with axonal and/or myelin markers. Surprisingly, our analysis of NGFR+ profiles uncovered the existence of at least 3 different novel populations of NGFR+/S100β− cells, herein referred to as nonglial cells, residing in the stroma and perivascular areas of all nerve compartments. An important proportion of the nerve's cellular content, including circa 30% of endoneurial cells, consisted of heterogenous S100β negative cells that were not associated with axons. Useful markers to identify the localization and diversity of nonglial cell types across different compartments were Thy1, CD34, SMA, and Glut1, a perineurial cell marker.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Interpretation</h3>\u0000 \u0000 <p>Our optimized methods revealed additional detailed information to update our understanding of the complexity and spatial orientation of PNS-resident cell types in humans.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17451,"journal":{"name":"Journal of the Peripheral Nervous System","volume":"29 3","pages":"294-314"},"PeriodicalIF":3.9,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of the Peripheral Nervous System
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1