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The effect of serum on human marrow mononuclear cell proliferation and maturation. 血清对人骨髓单核细胞增殖和成熟的影响。
C D Alley, R P MacDermott, C C Stewart

The effect of different serum sources on the growth of human bone marrow mononuclear cells in liquid culture was investigated. Newborn calf serum failed to support growth in liquid cultures whether or not exogenous colony-stimulating factor was present. Neither adherent nor nonadherent cells proliferated in medium supplemented with horse serum. Fetal calf serum allowed proliferation of the nonadherent cell population, but only in the presence of colony-stimulating factor. However, no growth of adherent cells was observed in these cultures. Both the nonadherent and adherent populations grew well in the presence of pooled human sera. While growth of the nonadherent population was minimal in the absence of colony-stimulating factor, the adherent population appeared to increase to a greater extent when the factor was absent. The positive effect of the human serum was shown to be dose dependent in that as the proportion of serum was increased in the medium, the number of cells recovered from the cultures increased, regardless of the presence or absence of exogenous colony-stimulating factor.

研究了不同血清来源对人骨髓单核细胞液体培养生长的影响。无论是否存在外源性集落刺激因子,新生牛犊血清都不能支持液体培养物的生长。在添加马血清的培养基中,贴壁细胞和非贴壁细胞均未增殖。胎牛血清允许非贴壁细胞群增殖,但只有在存在集落刺激因子的情况下。然而,在这些培养中没有观察到贴壁细胞的生长。在汇集的人类血清中,非粘附种群和粘附种群都生长良好。在没有集落刺激因子的情况下,非粘附种群的增长最小,而在没有集落刺激因子的情况下,粘附种群的增长似乎更大。人血清的积极作用被证明是剂量依赖的,因为随着培养基中血清比例的增加,从培养中恢复的细胞数量增加,无论是否存在外源性集落刺激因子。
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引用次数: 0
Effects of Mycobacterium bovis (strain BCG) on the interstitial cells of hydronephrotic, contralateral, and normal rabbit kidneys. 牛分枝杆菌(卡介苗)对肾积水、对侧和正常兔肾间质细胞的影响。
D L Thomasson, S Buck, T V Zenser, B B Davis

Studies were undertaken to determine the effect of viable organisms of Mycobacterium bovis, strain Bacillus Calmette Guerin (BCG), on cell growth characteristics and phagocytic properties of cells from surgically-induced unilaterally hydronephrotic, contralateral, and normal rabbit kidneys. A single intravenous administration of 8 X 10(8) BCG organisms was given at the time of ureteral ligation. Four days after injection, explants were removed from the hydronephrotic, contralateral, and normal kidneys. Two cell types, fibroblasts and mononuclear phagocytes, grew from these explants. BCG caused a marked increase in the rate of growth of cells from the hydronephrotic and contralateral kidneys. There was no measurable effect of BCG on cells from the normal kidney.

研究确定了牛分枝杆菌,卡介苗菌株(BCG)的活菌对手术诱导的单侧肾、对侧肾和正常兔肾脏细胞的生长特性和吞噬特性的影响。输尿管结扎时单次静脉注射8 × 10(8)个卡介苗。注射后4天,从肾积水、对侧和正常肾脏中取出外植体。两种细胞类型,成纤维细胞和单核吞噬细胞,从这些外植体中生长出来。卡介苗引起肾积水和对侧肾细胞生长速率的显著增加。卡介苗对正常肾细胞无明显影响。
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引用次数: 0
Kinetic analysis of the growth of bone marrow mononuclear phagocytes in long-term cultures. 长期培养骨髓单核吞噬细胞生长的动力学分析。
R van Furth, J W van der Meer, H Toivonen, T Rytömaa

The present work concerns a mathematical analysis of the growth of monoblasts, promonocytes, and macrophages in long-term bone marrow cultures in the presence of conditioned medium. For this purpose, use was made of the normalized data of four experiments, each done in triplicate. The computer program was based on a concept of hypothetical subcompartments within each developmental stage. The growth parameters were then determined experimentally or by trial and error after a series of computer simulations. The mathematical results are in close agreement with the numbers of monoblasts, promonocytes, and macrophages obtained by counts in 21-day-old bone marrow cultures. This approach provides a means to understand the kinetic behaviour of mononuclear phagocytes.

目前的工作涉及在条件培养基存在下的长期骨髓培养中单核细胞、单核细胞和巨噬细胞生长的数学分析。为此,使用了四个实验的归一化数据,每个实验都做了三份。计算机程序是基于每个发育阶段的假想小隔间的概念。然后通过实验或一系列计算机模拟后的试错来确定生长参数。数学结果与单核细胞、单核细胞和巨噬细胞在21日龄骨髓培养中计数的数量密切一致。这种方法为了解单核吞噬细胞的动力学行为提供了一种手段。
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引用次数: 0
Characteristics of long-term cultures of proliferating, mononuclear phagocytes from bone marrow. 骨髓中增殖的单核吞噬细胞长期培养的特点。
J W van der Meer, J S van de Gevel, R van Furth

The characteristics of murine bone marrow mononuclear phagocytes in long-term cultures with embryonic fibroblast-conditioned medium were studied to determine the stage of development and state of activation of these cells. Two liquid culture systems were used: for studies on the morphology, cytochemistry, and functional characteristics at the cellular level, the cells were cultured adherent to a glass surface; and for experiments where the cells were needed in suspension (replating experiments, and studies on locomotion, intracellular killing, and cytotoxicity) use was made of Teflon culture systems. Three developmental stages of mononuclear phagocytes could be recognized easily in these cultures: monoblasts, promonocytes, and macrophages. In cultures on a glass surface, these cells grow in colonies separate from granulocytic colonies. When incubation is prolonged beyond 7-9 days, the granulocytes die, leaving pure mononuclear phagocyte cultures. Primary cultures, in which monoblasts, promonocytes, and some macrophages proliferate, can be maintained for 3-4 weeks. Calculation showed that one monoblast present on day 0 gives rise to a progeny of more than 7 X 10(3) mononuclear phagocytes by day 14; after that, the rate of proliferation declines despite the addition of fresh media. Regular replating of the cells cultured on Teflon made it possible to maintain proliferation over a period of almost 200 days. The cells in culture have the typical characteristics of mononuclear phagocytes, as judged by light microscopy, alpha-naphthyl butyrate esterase activity, lysozyme activity, presence of receptors for Fc and C3, and endocytic, microbicidal, and cytotoxic activity. The 5'nucleotidase activity, ingestion of erythrocytes via C3-receptor, locomotion, and antibody-dependent cytotoxicity indicate that the cultured bone marrow mononuclear phagocytes are more active than resident macrophages, and as active as or even more active than thioglycollate-induced macrophages. In conclusion, the population of mononuclear phagocytes in the liquid cultures of bone marrow is heterogenous with respect to developmental stage and state of activation.

研究了小鼠骨髓单核吞噬细胞在胚胎成纤维细胞条件培养基中长期培养的特点,以确定这些细胞的发育阶段和活化状态。采用两种液体培养系统:在细胞水平上研究细胞形态、细胞化学和功能特征,将细胞贴壁培养在玻璃表面;对于需要悬浮细胞的实验(复制实验、运动、细胞内杀伤和细胞毒性研究),使用聚四氟乙烯培养系统。在这些培养物中可以很容易地识别出单核吞噬细胞的三个发育阶段:单核细胞、单核细胞和巨噬细胞。在玻璃表面的培养中,这些细胞在与粒细胞菌落分离的菌落中生长。当孵育超过7-9天,粒细胞死亡,留下纯单核吞噬细胞培养物。原代培养可维持3-4周,其中单核细胞、单核细胞和部分巨噬细胞增殖。计算表明,第0天存在的一个单核细胞在第14天产生了超过7 × 10(3)个单核吞噬细胞的后代;在此之后,尽管添加了新鲜培养基,但增殖速度下降。在聚四氟乙烯上培养的细胞定期复制,可以在近200天的时间内保持增殖。培养的细胞具有典型的单核吞噬细胞的特征,通过光镜判断,α -丁酸萘酯酶活性,溶菌酶活性,Fc和C3受体的存在,以及内吞,杀微生物和细胞毒性活性。5′核苷酸酶活性、红细胞通过c3受体摄取、运动和抗体依赖的细胞毒性表明,培养的骨髓单核吞噬细胞比驻留的巨噬细胞更活跃,与巯基乙酸盐诱导的巨噬细胞一样活跃,甚至更活跃。综上所述,骨髓液体培养中的单核吞噬细胞在发育阶段和激活状态方面具有异质性。
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引用次数: 0
Distribution of peritoneal macrophage populations after intravenous injection in mice: differential effects of eliciting and activating agents. 小鼠静脉注射后腹腔巨噬细胞群的分布:诱导剂和激活剂的不同作用。
R H Wiltrout, M J Brunda, E Gorelik, E S Peterson, J J Dunn, J Leonhardt, L Varesio, C W Reynolds, H T Holden

Murine peritoneal macrophages (pM phi) elicited in vivo by intraperitoneal (IP) inoculation of various agents were tested for their homing/distribution patterns after intravenous (IV) adoptive transfer to syngeneic C57BL/6 recipients. Resident pM phi (RpM phi) obtained from normal mice and pM phi elicited by proteose peptone (PpM phi) or thioglycollate broth (TpM phi) exhibited similar homing patterns following IV transfer. After initial arrest in the lungs, these cells rapidly disseminated to liver and spleen, with minimal or no detectable migration to peripheral lymph nodes, intestine, peritoneum, kidney, heart, or retention in the blood. The pattern of results reflected the properties of pM phi themselves, since highly enriched pM phi populations obtained by treatment of crude peritoneal exudate cells with anti-Thy 1.2 + C, or by fractionation on Percoll density gradients, gave similar results. The distribution of pM phi elicited by Brewer's thioglycollate medium (BTpM phi) was markedly different from other pM phi tested. BTpM phi homed rapidly to the lungs and many remained localized there for at least 72 hr with very little migration to the spleen. The distribution of PpM phi could be altered by activation of these cells in vivo through the IP injection of the pyran copolymer, MVE-2, prior to adoptive IV transfer. Activated PpM phi contained a population of highly differentiated, low density pM phi, separable on density gradients, which arrested in the lungs for appreciably longer periods of time than did PpM phi. These cells exhibited reduced ability for migration to the spleen. Macrophage-like (M phi-like) cell lines did not exhibit migration capability, but rather were rapidly cleared from the circulation in a manner similar to other types of tumor cells.

通过腹腔(IP)接种多种药物在体内诱导小鼠腹腔巨噬细胞(pM phi),并通过静脉(IV)过继转移到同源C57BL/6受体,检测其归巢/分布模式。从正常小鼠获得的常驻pM phi (RpM phi)和由蛋白酶蛋白胨(PpM phi)或巯基酸盐肉汤(TpM phi)诱导的pM phi在IV传递后表现出相似的归巢模式。在肺部最初停止后,这些细胞迅速播散到肝脏和脾脏,很少或未检测到迁移到周围淋巴结、肠、腹膜、肾脏、心脏或保留在血液中。结果的模式反映了pM phi本身的特性,因为用抗thy 1.2 + C处理粗腹膜渗出细胞或在Percoll密度梯度上分离获得的高度富集的pM phi群体给出了类似的结果。Brewer's thioglycollate培养基(BTpM phi)诱导的pM phi分布与其他测试的pM phi有明显不同。BTpM phi迅速回到肺部,许多在那里停留至少72小时,很少迁移到脾脏。在继代IV转移之前,通过IP注射pyran共聚物MVE-2,可以在体内激活这些细胞,从而改变PpM phi的分布。激活的PpM phi包含一个高度分化的种群,低密度的pM phi,在密度梯度上是可分离的,它在肺部停留的时间比PpM phi明显更长。这些细胞表现出向脾脏迁移的能力降低。巨噬细胞样细胞系不表现出迁移能力,而是以类似于其他类型肿瘤细胞的方式迅速从循环中清除。
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引用次数: 0
Kinetics of killing Listeria monocytogenes by macrophages: rapid killing accompanying phagocytosis. 巨噬细胞杀死单核增生李斯特菌的动力学:伴随吞噬作用的快速杀伤。
W A Davies

The kinetics of bactericidal activity of activated macrophages can be precisely described by a mathematical model in which phagocytosis, killing, digestion, and release of degraded bacterial material are considered to occur continuously. To gain a better understanding of these events, I have determined the period of time between first contact of bacteria with macrophages and the onset of killing. Activated rat peritoneal macrophages were incubated for various times up to 15 min with Listeria monocytogenes previously labeled with 3H-thymidine and the unassociated bacteria removed by two centrifugations through a density interface. Both cell-associated radioactivity and cell-associated viable bacteria, determined as colony forming units after sonication of the cell pellet, increased with time of incubation. However, the specific viability of these bacteria, expressed as the ratio of number of viable bacteria per unit radioactivity declined with time, as an approximate inverse exponential, after a lag period of 2.9 +/- 0.8 min. Evidence is given that other possible causes for this decline in specific viability, other than death of the bacteria, such as preferential ingestion of dead Listeria, clumping of bacteria, variations in autolytic activity, or release of Listericidins are unlikely. I conclude therefore that activated macrophages kill Listeria approximately 3 min after the cell and the bacterium first make contact.

活化的巨噬细胞的杀菌活性动力学可以用一个数学模型精确地描述,在这个模型中,吞噬、杀死、消化和释放降解的细菌物质被认为是连续发生的。为了更好地理解这些事件,我确定了从细菌与巨噬细胞第一次接触到开始杀伤之间的时间。活化的大鼠腹腔巨噬细胞与单核增生李斯特菌(Listeria monocytogenes)孵育不同时间,孵育时间最长为15分钟,李斯特菌先前用3h -胸腺嘧啶标记,通过密度界面两次离心去除不相关的细菌。细胞相关的放射性和细胞相关的活菌,在超声处理后被确定为集落形成单位,随着孵育时间的延长而增加。然而,在2.9 +/- 0.8分钟的滞后期后,这些细菌的比活力(以单位放射性活菌数之比表示)以近似的逆指数形式随时间下降。证据表明,除细菌死亡外,其他可能导致比活力下降的原因,如优先摄入死亡的李斯特菌、细菌结块、自溶活性的变化或李斯特菌素的释放不太可能。因此,我得出结论,激活的巨噬细胞在细胞和细菌第一次接触后大约3分钟杀死李斯特菌。
{"title":"Kinetics of killing Listeria monocytogenes by macrophages: rapid killing accompanying phagocytosis.","authors":"W A Davies","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The kinetics of bactericidal activity of activated macrophages can be precisely described by a mathematical model in which phagocytosis, killing, digestion, and release of degraded bacterial material are considered to occur continuously. To gain a better understanding of these events, I have determined the period of time between first contact of bacteria with macrophages and the onset of killing. Activated rat peritoneal macrophages were incubated for various times up to 15 min with Listeria monocytogenes previously labeled with 3H-thymidine and the unassociated bacteria removed by two centrifugations through a density interface. Both cell-associated radioactivity and cell-associated viable bacteria, determined as colony forming units after sonication of the cell pellet, increased with time of incubation. However, the specific viability of these bacteria, expressed as the ratio of number of viable bacteria per unit radioactivity declined with time, as an approximate inverse exponential, after a lag period of 2.9 +/- 0.8 min. Evidence is given that other possible causes for this decline in specific viability, other than death of the bacteria, such as preferential ingestion of dead Listeria, clumping of bacteria, variations in autolytic activity, or release of Listericidins are unlikely. I conclude therefore that activated macrophages kill Listeria approximately 3 min after the cell and the bacterium first make contact.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 2","pages":"131-41"},"PeriodicalIF":0.0,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17471839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification by flow cytometry of human monocytes with fucose-binding lectin (FBL) from Lotus tetragonolobus seeds. 用流式细胞术鉴定荷花种子凝集素(FBL)对人单核细胞的影响。
D E Van Epps, R Brazil, K Tung, N Warner

Fucose binding lectin (FBL) from Lotus tetragonolobus seeds has previously been shown by fluorescence microscopy to bind to human neutrophils. This study shows using highly sensitive flow cytometry that this lectin binds both to human peripheral blood neutrophils and monocytes but not to lymphocytes. This binding is blocked by the presence of free L-fucose and is reversible when neutrophils or monocytes stained with fluorescent FBL are subsequently incubated in 0.05 M L-fucose. Quantitative comparison of neutrophils and monocytes from the same individual show that neutrophils bind approximately 2.6 times more FBL than monocytes and that FBL binding is more efficiently reversed with neutrophils, as compared with monocytes, by L-fucose. Additional double-labeling studies of cells with FBL and the OKM1 monoclonal antibody, which identifies monocytes and granulocytes, show that all cells binding FBL also stain with the OKM1 monoclonal antibody. This study shows that qualitatively, FBL may be utilized as a human myeloid cell marker to differentiate peripheral blood monocytes and neutrophils from lymphocytes.

荧光显微镜已经证实,莲子中的聚焦结合凝集素(FBL)可以与人中性粒细胞结合。本研究使用高灵敏度流式细胞术显示,这种凝集素与人外周血中性粒细胞和单核细胞结合,但不与淋巴细胞结合。这种结合被游离的L-病灶阻断,当中性粒细胞或单核细胞被荧光FBL染色后在0.05 M L-病灶中孵育时,这种结合是可逆的。对来自同一个体的中性粒细胞和单核细胞的定量比较表明,中性粒细胞结合FBL的能力大约是单核细胞的2.6倍,并且与单核细胞相比,中性粒细胞通过L-聚焦更有效地逆转FBL的结合。另外对FBL细胞和OKM1单克隆抗体(可识别单核细胞和粒细胞)的双标记研究表明,所有结合FBL的细胞也被OKM1单克隆抗体染色。本研究定性地表明,FBL可作为人骨髓细胞的标志物,用于区分外周血单核细胞和中性粒细胞与淋巴细胞。
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引用次数: 0
Replication of respiratory syncytial virus in lungs of immunodeficient mice. 呼吸道合胞病毒在免疫缺陷小鼠肺中的复制。
P R Wyde, C S Sun, V Knight

Respiratory syncytial virus was frequently isolated during a 10-day test period from the lungs of 4- to 6-week-old immunodeficient nude (nu/nu) mice and from gamma-irradiated C3H mice inoculated intranasally with this virus, but not from similar aged and comparably inoculated normal littermates of these mice. Virus isolation rates and levels of virus in lungs in both groups of immunodeficient mice were similar. No extrapulmonary dissemination of virus was observed in any test group of mice.

在为期10天的试验期间,经常从4至6周龄免疫缺陷裸(nu/nu)小鼠的肺部和经鼻内接种该病毒的γ辐照C3H小鼠中分离出呼吸道合胞体病毒,但没有从这些小鼠的相似年龄和同样接种的正常窝伴中分离出。两组免疫缺陷小鼠的病毒分离率和肺部病毒水平相似。各组小鼠均未见病毒肺外传播。
{"title":"Replication of respiratory syncytial virus in lungs of immunodeficient mice.","authors":"P R Wyde,&nbsp;C S Sun,&nbsp;V Knight","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Respiratory syncytial virus was frequently isolated during a 10-day test period from the lungs of 4- to 6-week-old immunodeficient nude (nu/nu) mice and from gamma-irradiated C3H mice inoculated intranasally with this virus, but not from similar aged and comparably inoculated normal littermates of these mice. Virus isolation rates and levels of virus in lungs in both groups of immunodeficient mice were similar. No extrapulmonary dissemination of virus was observed in any test group of mice.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 2","pages":"125-9"},"PeriodicalIF":0.0,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17936657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of calcium-dependent proteolytic activity in human polymorphonuclear leukocytes. 人多形核白细胞钙依赖性蛋白水解活性的鉴定。
J L Legendre, H P Jones

Calcium-dependent proteolytic activity has been identified in extracts of human polymorphonuclear leukocytes. The activity is most pronounced in the neutral pH range with a pH optimum of 7.3. Maximal activation of the protease occurs at a free calcium concentration of 190 microM; it is half maximal at 91 microM. This protease activity is strongly inhibited by aprotinin and phenylmethylsulfonyl fluoride (PMSF) and more weakly inhibited by antipain, leupeptin, and o-phenanthroline. The protease is not activated by calmodulin nor is it inhibited by the calmodulin antagonist trifluoperazine. Gel filtration suggests a molecular weight of 74,100 daltons.

钙依赖的蛋白水解活性已经在人类多形核白细胞提取物中被鉴定出来。活性在中性pH范围内最明显,pH值最适为7.3。蛋白酶的最大活化发生在游离钙浓度为190微米时;在91微米时是最大值的一半。抑肽蛋白和苯基甲基磺酰氟(PMSF)强烈抑制该蛋白酶的活性,而抗疼痛、白细胞介素和邻菲罗啉的抑制作用较弱。蛋白酶不能被钙调素激活,也不能被钙调素拮抗剂三氟拉嗪抑制。凝胶过滤表明其分子量为74100道尔顿。
{"title":"Identification of calcium-dependent proteolytic activity in human polymorphonuclear leukocytes.","authors":"J L Legendre,&nbsp;H P Jones","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Calcium-dependent proteolytic activity has been identified in extracts of human polymorphonuclear leukocytes. The activity is most pronounced in the neutral pH range with a pH optimum of 7.3. Maximal activation of the protease occurs at a free calcium concentration of 190 microM; it is half maximal at 91 microM. This protease activity is strongly inhibited by aprotinin and phenylmethylsulfonyl fluoride (PMSF) and more weakly inhibited by antipain, leupeptin, and o-phenanthroline. The protease is not activated by calmodulin nor is it inhibited by the calmodulin antagonist trifluoperazine. Gel filtration suggests a molecular weight of 74,100 daltons.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 2","pages":"89-97"},"PeriodicalIF":0.0,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17411499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leprosy in wild armadillos (Dasypus novemcinctus) of the Texas Gulf Coast: epidemiology and mycobacteriology. 德克萨斯海湾沿岸野生犰狳(Dasypus novemcintus)的麻风病:流行病学和分枝细菌学。
J H Smith, D S Folse, E G Long, J D Christie, D T Crouse, M E Tewes, A M Gatson, R L Ehrhardt, S K File, M T Kelly

A significant prevalence of leprosy has been demonstrated in wild Louisiana armadillos. The Texas Gulf Coast still has endemic human leprosy, and recent mores in Texas have markedly increased armadillo-human contact. Armadillos were screened by physical examination, and by ear-snip and slit-scrape technique. Animals that screened "positive" were sacrificed and necropsied under aseptic conditions. Liver, spleen, gross lesions, and four groups of lymph nodes were cultured for mycobacteria and were studied histologically. Base ratios and DNA homology with Mycobacterium leprae were determined on mycobacteria from two armadillos (and two tissues from one of these); these studies indicate that the organism found in Texas armadillos is M leprae. Twenty-one of the armadillos were leprous--4.66%. The local prevalence varied from 1.0% to 15.4%. Epidemiologic implications of these findings and the occurrence of other concomitant mycobacterial infections are discussed.

麻疯病在野生路易斯安那犰狳中有显著的流行。德克萨斯州墨西哥湾沿岸仍然有地方性的人类麻风病,最近德克萨斯州的一些活动明显增加了犰狳与人类的接触。对犰狳进行体格检查,并采用剪耳和刮耳技术进行筛选。筛选“阳性”的动物被处死并在无菌条件下进行尸检。对肝、脾、大体病变及四组淋巴结进行分枝杆菌培养及组织学观察。对来自两只犰狳的分枝杆菌(以及其中一只犰狳的两个组织)进行了碱基比和与麻风分枝杆菌的DNA同源性测定;这些研究表明,在德克萨斯犰狳身上发现的微生物是麻风杆菌。21只犰狳患麻风,占4.66%。当地患病率为1.0% ~ 15.4%。这些发现的流行病学意义和其他并发分枝杆菌感染的发生进行了讨论。
{"title":"Leprosy in wild armadillos (Dasypus novemcinctus) of the Texas Gulf Coast: epidemiology and mycobacteriology.","authors":"J H Smith,&nbsp;D S Folse,&nbsp;E G Long,&nbsp;J D Christie,&nbsp;D T Crouse,&nbsp;M E Tewes,&nbsp;A M Gatson,&nbsp;R L Ehrhardt,&nbsp;S K File,&nbsp;M T Kelly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A significant prevalence of leprosy has been demonstrated in wild Louisiana armadillos. The Texas Gulf Coast still has endemic human leprosy, and recent mores in Texas have markedly increased armadillo-human contact. Armadillos were screened by physical examination, and by ear-snip and slit-scrape technique. Animals that screened \"positive\" were sacrificed and necropsied under aseptic conditions. Liver, spleen, gross lesions, and four groups of lymph nodes were cultured for mycobacteria and were studied histologically. Base ratios and DNA homology with Mycobacterium leprae were determined on mycobacteria from two armadillos (and two tissues from one of these); these studies indicate that the organism found in Texas armadillos is M leprae. Twenty-one of the armadillos were leprous--4.66%. The local prevalence varied from 1.0% to 15.4%. Epidemiologic implications of these findings and the occurrence of other concomitant mycobacterial infections are discussed.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 2","pages":"75-88"},"PeriodicalIF":0.0,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17411498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of the Reticuloendothelial Society
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