Pub Date : 2018-07-16DOI: 10.17951/C.2017.72.1.15-26
E. Kochan, Sylwia Caban, G. Szymańska, P. Szymczyk, A. Lipert, P. Kwiatkowski, Monika Sienkiewicz
Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.
植物悬浮培养被描述为获得药用次生代谢物的来源，在未来可能成为传统原料的替代品。本研究证实了人参的一种——西洋参(Panax quinquefolium L.)的细胞培养可合成人参皂苷，这是一种具有多向药理作用的三萜皂苷。测试的悬浮培养液在摇瓶中小规模运行，并在10升搅拌槽中按比例放大过程。在摇瓶中，培养第30天生物量产量最高(干重2.28 g -1，鲜重33.99 g -1)，总皂苷含量最高(2.66 mg g -1 dw)。在生物反应器中，相对于接种量，干生物量和鲜生物量分别增加了近2.67倍和3倍。大规模培养合成了原人参二醇衍生物，如Rg1和Re人参皂苷，但没有报道属于原人参二醇衍生物的皂苷。
{"title":"Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor","authors":"E. Kochan, Sylwia Caban, G. Szymańska, P. Szymczyk, A. Lipert, P. Kwiatkowski, Monika Sienkiewicz","doi":"10.17951/C.2017.72.1.15-26","DOIUrl":"https://doi.org/10.17951/C.2017.72.1.15-26","url":null,"abstract":"Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.","PeriodicalId":177112,"journal":{"name":"Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133759248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-16DOI: 10.17951/c.2017.72.1.37-45
Samaneh Yari, N. Sabaghnia, M. Pasandi, M. Janmohammadi
Effective interpretation of the data on breeding programs is important at all stages of plant improvement and the genotype by trait (GT) biplot was used for two-way wheat dataset as genotypes with multiple traits. For this propose, 18 rye genotypes with specific characteristics were evaluated in randomized block design with four replications. The GT biplot for rye dataset explained 61% of the total variation of the standardized data (the first two principal components explained 40 and 21% respectively). The polygon view of GT presented for 11 different traits of rye cultivars showed six vertex cultivars as G1, G3, G6, G8, G11 and G13 whose genotype G8 had the highest values for most of the measured traits. Generally based on vector view, ideal genotype and ideal tester biplots, it was demonstrated that the selection of high seed yield will be performed via seed number per spike, first internode weight, number of spike per area and harvest index. These traits should be considered simultaneously as effective selection criteria evolving high yielding rye cultivars because of their large contribution to seed yield. The genotypes G8 and G7 following to genotypes G3, G18 and G19 could be considered for the developing of desirable progenies in the selection strategy of rye improvement programs.
{"title":"Assessment of genotype × trait interaction of rye genotypes for some morphologic traits through GGE biplot methodology","authors":"Samaneh Yari, N. Sabaghnia, M. Pasandi, M. Janmohammadi","doi":"10.17951/c.2017.72.1.37-45","DOIUrl":"https://doi.org/10.17951/c.2017.72.1.37-45","url":null,"abstract":"Effective interpretation of the data on breeding programs is important at all stages of plant improvement and the genotype by trait (GT) biplot was used for two-way wheat dataset as genotypes with multiple traits. For this propose, 18 rye genotypes with specific characteristics were evaluated in randomized block design with four replications. The GT biplot for rye dataset explained 61% of the total variation of the standardized data (the first two principal components explained 40 and 21% respectively). The polygon view of GT presented for 11 different traits of rye cultivars showed six vertex cultivars as G1, G3, G6, G8, G11 and G13 whose genotype G8 had the highest values for most of the measured traits. Generally based on vector view, ideal genotype and ideal tester biplots, it was demonstrated that the selection of high seed yield will be performed via seed number per spike, first internode weight, number of spike per area and harvest index. These traits should be considered simultaneously as effective selection criteria evolving high yielding rye cultivars because of their large contribution to seed yield. The genotypes G8 and G7 following to genotypes G3, G18 and G19 could be considered for the developing of desirable progenies in the selection strategy of rye improvement programs.","PeriodicalId":177112,"journal":{"name":"Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124331023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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