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Asian Conference on Biomedical Research and Laboratory Medicine 2021 2021年亚洲生物医学研究和实验室医学会议
Pub Date : 2022-04-18 DOI: 10.28916/lsmb.6.1.2022.105
W. A. N. Wan Ahmad, M. Kaderi, S. F. Masre, S. B. Budin, U. Kuppusamy, Raja Elina Raja Aziddin, S. N. E. Sarchio, S. Sukardi, Abdah Md Akim, Y. Cheah, N. Rajab, Izatus Shima Taib, Farah Wahida Ibrahim, N. Jufri, C. Lim, Harmiza Harun
Asian Conference on Biomedical Research and Laboratory Medicine was organized by three associations: Malaysian Association of Clinical Biochemists (MACB), Association of Scientific Officers Ministry of Health (ASOMH), and Malaysian Biomedical Science Association (MyBIOMED). The conference was held on 24th-25th August 2021 and a workshop on 26th August 2021 through Webex Webinar Platform with the theme of “Revolutionising laboratory medicine through research and innovation”. This conference aimed to bring together leading academic, scientists, laboratory medical scientists, pathologists, researchers, and research scholars to exchange and share their experiences and research results about all aspects of laboratory medicine and health sciences. There were a total of almost 500 participants registered and over 100 abstracts submitted for this conference.
亚洲生物医学研究和实验室医学会议由三个协会组织:马来西亚临床生物化学家协会(MACB)、卫生部科学官员协会(ASOMH)和马来西亚生物医学科学协会(MyBIOMED)。会议于2021年8月24日至25日举行,并于2021年8月26日通过Webex网络研讨会平台举办研讨会,主题为“通过研究和创新革新检验医学”。本次会议旨在汇集领先的学术、科学家、检验医学科学家、病理学家、研究人员和研究学者,交流和分享他们在检验医学和健康科学各个方面的经验和研究成果。本次会议共有近500名与会者注册,并提交了100多份摘要。
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引用次数: 0
Myocardial infarction in normal coronary artery (MINCA): Death in healthy military personnel 正常冠状动脉心肌梗死(MINCA):健康军人死亡
Pub Date : 2022-04-15 DOI: 10.28916/lsmb.6.1.2022.90
Nadiawati Abdul Razak, F. Mohd Nor, Nur Najmi Mohamad Anuar, Ahmad Zakuan Kamarudin
Sudden unexpected death among healthy military personnel leads to a false allegation from family members. However, majority of the deaths were related to an abnormal cardiac condition. Objective: The occurrence of patent coronary vessels in sudden deaths demands histological examination and/or molecular study to detect underlying diseases, which need to be highlighted especially in a centre with limitation of post-mortem computed tomography (PMCT) or virtopsy. In this article, a case of a young healthy male personnel, who died during routine training was reported. He collapsed after a 10-minute jog. An autopsy was performed to determine the cause of death. The autopsy showed a moderately built male. No fatal external injuries were found. Internal examination showed cardiomegaly and patent coronaries. The cardiac markers were markedly raised with normal toxicological analysis. Histology of the heart showed a recent myocardial infarct, hence the cause of death was determined as myocardial infarction in normal coronary artery (MINCA). Conclusion: Annual medical screening is highly recommended to detect any cardiac abnormalities among military personnel. Screening may be extended for a genetic study in suspicious and strong family history to exclude a conduction defect or channelopathy.
健康的军事人员突然意外死亡导致家属提出虚假指控。然而,大多数死亡与心脏异常有关。目的:猝死患者冠脉未闭的发生需要通过组织学检查和/或分子研究来发现潜在疾病,特别是在尸检计算机断层扫描(PMCT)或虚拟解剖技术有限的中心,这一点需要得到重视。本文报告了一例年轻健康男性工作人员在日常训练中死亡的病例。慢跑10分钟后,他晕倒了。为了确定死因,进行了尸检。尸检显示是一个中等身材的男性。没有发现致命的外部伤害。内部检查显示心脏肥大,冠状动脉未闭。心脏指标明显升高,毒理学分析正常。心脏组织学显示近期心肌梗死,故确定死因为正常冠状动脉心肌梗死。结论:强烈建议军人每年进行一次体检,以发现任何心脏异常。筛查可扩展到可疑和强烈家族史的遗传研究,以排除传导缺陷或通道病。
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引用次数: 1
Microencapsulation of Lysiphyllum strychnifolium extract using pectin as a carrier matrix and its characterization 以果胶为载体的马钱兰提取物微胶囊化及其表征
Pub Date : 2022-04-03 DOI: 10.28916/lsmb.6.1.2022.102
Arman Syah Goli, J. Leanpolchareanchai, S. Chewchinda, J. Yahuafai, Jannarin Nontakham, H. Sato, V. Sato
Lysiphyllum strychnifolium (Craib) A. Schmitz (LS, Fabaceae) is one of the folklore medicines in Thailand. The previous studies have demonstrated several pharmacological activities and high polyphenolic substances possessed by this plant. However, the suitable encapsulation of LS extract has not been discovered. This study aimed to develop LS microcapsules using spray-drying technique with pectin as a carrier. Moreover, the powder analysis and characterization were also conducted. The effects of inlet temperatures (80, 100, and 120°C) and carrier concentrations (1, 5, and 10 %w/v) on the encapsulation yield (EY), encapsulation efficiency (EE), total phenolic content (TPC), and main markers (trilobatin and yanangdaengin) of LS microcapsules were studied. Finally, the characterization was investigated by Fourier transform infrared (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). The obtained results indicated that S2 microcapsule formulation, pectin to extract ratio 10:1 (w/w) at inlet temperature of 100°C, was chosen as the optimal condition because of the positive tendency to acquire higher EE as pectin level was increased. On the contrary, the level of TPC and markers was reduced due to the more addition of pectin. The FTIR, XRD, and DSC results suggested that the well-encapsulated microcapsules were obtained for S2 formulation and SEM represented the semi-spherical structure of its microstructures. The development of LS microcapsules with the proximity to gain the advantageous powder analysis and characteristic has been achieved. Therefore, this approach could be used for the subsequent manufacturing of LS extract.
马钱兰(Lysiphyllum strychnifolium) (LS, Fabaceae)是泰国的民间药材之一。以往的研究表明,该植物具有多种药理活性和高多酚类物质。然而,目前还没有找到合适的包封方法。以果胶为载体,采用喷雾干燥技术制备LS微胶囊。此外,还对粉末进行了分析和表征。研究了进口温度(80、100和120℃)和载体浓度(1、5和10% w/v)对LS微胶囊的包封率(EY)、包封效率(EE)、总酚含量(TPC)和主要标记物(三叶草苷和杨柳糖苷)的影响。最后,利用傅里叶变换红外(FTIR)、x射线衍射(XRD)、差示扫描量热法(DSC)和扫描电镜(SEM)对其进行表征。结果表明,在进口温度为100℃的条件下,果胶与提取物比为10:1 (w/w)的S2微胶囊配方具有随着果胶添加量的增加而获得更高EE的趋势。相反,随着果胶添加量的增加,TPC和标记物的含量降低。FTIR、XRD和DSC分析结果表明,S2配方获得了包封良好的微胶囊,SEM表征了其微观结构的半球形结构。研制了具有接近性的LS微胶囊,获得了良好的粉末分析和特性。因此,该方法可用于LS提取物的后续生产。
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引用次数: 1
Traffic light profiling of meals sold in cafeteria of local universities: A pilot study 地方大学食堂出售食物的红绿灯分析:一项试点研究
Pub Date : 2022-03-26 DOI: 10.28916/lsmb.6.1.2022.88
Shi Yee Loo, Anis Wahida Md Nazri, Nur Eizzati Farhani Rosle, Munirah Ismail, Yi Yi Lee, S. Krishnasamy
Unregulated university cafeterias may be serving food and beverages high in fat or sugar. Traffic light labelling (TLL) has been able to guide students in making healthier choices. Therefore, the aim of this study was to assess the healthiness level of food and beverages served in university cafeterias by profiling it against TLL and calorie density to evaluate student’s perception towards the implementation of TLL. This pilot observational study was conducted in three universities; two public university and one private institution within Klang Valley. A total of 166 foods and beverages were analysed for calories, fat, saturated fat, sodium, and sugar by using a nutritional software, Nutritionist Pro (TM). Analysed data for each food/beverage were further profiled into TLL and calorie density by using an excel spreadsheet. Food and beverages analysed were those sold in canteen and cafeterias combined those frequently consumed, reported in a food frequency questionnaire (FFQ). A total of 192 students completed a questionnaire containing three sections: personal profile, a short FFQ of their usual intake and their perception towards implementation of TLL in their institutions. 8.1% of all food items were categorised as green (healthy food), whereas 41.9% were amber (moderately healthy food), and 50% were red (unhealthy food). Almost half of the beverages (46.7%) were found to be unhealthy. However, when all foods were profiled for calorie density, more than half (61.8%) were categorised as moderately healthy food. In addition, most of the respondents (88%) showed a positive perception towards the implementation of TLL in the cafeteria. A large number of meals sold in two universities cafeterias were unhealthy and requires dietary modification. TLL implementation was beneficial for tertiary students as it may help guide the students to choose healthier options of cooked meals and packaged foods and beverages.
不受监管的大学食堂可能会提供高脂肪或高糖的食品和饮料。交通灯标签(TLL)能够引导学生做出更健康的选择。因此,本研究的目的是评估大学食堂提供的食品和饮料的健康水平,通过将其与TLL和卡路里密度进行对比,以评估学生对实施TLL的看法。这项初步观察性研究在三所大学进行;巴生谷的两所公立大学和一所私立大学。使用营养软件Nutritionist Pro (TM)分析了166种食品和饮料的卡路里、脂肪、饱和脂肪、钠和糖。通过使用excel电子表格,对每种食品/饮料的分析数据进一步分析为TLL和卡路里密度。所分析的食品和饮料是在食堂和自助餐厅出售的食品和饮料,以及在食物频率问卷(FFQ)中报告的经常消费的食品和饮料。共有192名学生完成了一份问卷,其中包括三个部分:个人简介,他们通常入学的简短FFQ以及他们对在其机构实施教学教学的看法。8.1%的食品被分类为绿色(健康食品),41.9%的食品被分类为琥珀色(中等健康食品),50%的食品被分类为红色(不健康食品)。几乎一半的饮料(46.7%)被发现是不健康的。然而,当对所有食物的卡路里密度进行分析时,超过一半(61.8%)的食物被归类为中等健康食品。此外,大多数受访者(88%)对在自助餐厅实施TLL表示积极的看法。两所大学食堂出售的大量食物不健康,需要调整饮食。TLL的实施对大学生是有益的,因为它可以帮助引导学生选择更健康的熟食和包装食品和饮料。
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引用次数: 0
Effects of fresh garlic extract on Candida albicans Sessile cells, biofilms and biofilm associated genes, Flo-8 and Ndt80 鲜大蒜提取物对白色念珠菌无柄细胞、生物膜及生物膜相关基因Flo-8和Ndt80的影响
Pub Date : 2022-02-20 DOI: 10.28916/lsmb.6.1.2022.89
Dave Thomas, Joshua Lee, S. Choo, H. Rao, P. Chong
Candida-associated nosocomial infections are a persistent problem which has been steadily increasing over the years. The emergence of antimicrobial resistant strains has narrowed the spectrum of effective drugs that can be utilised and indicated the need for alternative therapeutics. Garlic is a spice often studied for its unique characteristics. Most of its antimicrobial properties have been attributed to the presence of allicin, which makes up more than 70% of thiosulfinates extracted from garlic. The present study aimed to determine the effects of fresh garlic extract (FGE) towards the expression of two major biofilm genes, Flo-8 and Ndt80 produced by C. albicans that enable the morphogenesis of yeast from planktonic cells to biofilm.  Minimum concentration required for FGE to inhibit C. albicans was determined through agar well and disc diffusion assay. Effect of FGE towards preformed C. albicans biofilms was evaluated at ¼× and ½× inhibitory concentrations of FGE and Amphotericin B, respectively by XTT assay. RNA from FGE-treated C. albicans was extracted, reverse transcribed and analysed by Real-Time Polymerase Chain Reaction (RT-PCR) to determine FGE effects towards the expression of Flo-8 and Ndt80. Inhibitory concentration of FGE was determined to be 100 mg/mL. Both genes appeared to be upregulated in the presence of FGE with a higher upregulation noted with Flo-8 (67.34 and 30.20-folds) when compared against Ndt80 (5.55 and 1.79-folds) at 5 mg/mL and 10 mg/mL, respectively. It is surmised that C. albicans upregulated these genes as a survival mechanism in the presence of FGE. The findings from this study indicate that FGE could inhibit C. albicans growth and biofilms. Further research is required to determine the minimum inhibitory concentration (MIC) through broth microdilution and to understand the mechanism behind this phenomenon before it can be utilised as a potential therapeutic to combat Candida-associated nosocomial infections.
念珠菌相关的医院感染是一个持续存在的问题,多年来一直在稳步增加。抗菌素耐药菌株的出现缩小了可用有效药物的范围,并表明需要替代疗法。大蒜是一种香料,因其独特的特性而经常被研究。它的大部分抗菌特性归因于大蒜素的存在,大蒜中提取的硫代亚硫酸盐中有70%以上是大蒜素。本研究旨在确定新鲜大蒜提取物(FGE)对白色念珠菌产生的两个主要生物膜基因Flo-8和Ndt80表达的影响,这些基因使酵母从浮游细胞向生物膜的形态发生。通过琼脂孔和圆盘扩散试验确定了FGE抑制白色念珠菌所需的最低浓度。在FGE和两性霉素B的抑制浓度分别为¼×和½×时,采用XTT法评价FGE对预制白色念珠菌生物膜的影响。提取经FGE处理的白念珠菌RNA,进行逆转录,RT-PCR检测FGE对fl -8和Ndt80表达的影响。测定FGE的抑制浓度为100 mg/mL。FGE存在时,这两个基因都出现了上调,与Ndt80(5.55和1.79倍)相比,FGE在5 mg/mL和10 mg/mL时的上调幅度更高,其中Flo-8的上调幅度分别为67.34和30.20倍。据推测,白色念珠菌在FGE存在下上调这些基因是一种生存机制。本研究结果表明,FGE能抑制白色念珠菌的生长和生物膜的形成。需要进一步的研究来确定通过肉汤微量稀释的最低抑制浓度(MIC),并了解这一现象背后的机制,然后才能将其用作对抗念珠菌相关医院感染的潜在治疗方法。
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引用次数: 1
Polyethylene microplastics adversely affect airway patency 聚乙烯微塑料对气道通畅有不利影响
Pub Date : 2022-02-08 DOI: 10.28916/lsmb.6.1.2022.93
Nurshafida Adzlin Shamsul Anuar, L. Pang, S. Selvam, C. Gibbins, Ting Kang Nee
Human exposure to microplastics through inhalation has been widely reported in recent years. There is a paucity of work focusing on the direct effect of accumulation of microplastics in airways and how it may impact the respiratory function. This study aimed to investigate whether the exposure of microplastic would change the contractility of isolated airway smooth muscle tissue. Microplastics were obtained through milling of high-density polyethylene (HDPE) pellets by using a centrifugal mill. To confirm that the milled microplastic particle size range fell within the definition of microplastic, field-emission scanning electron microscope (FESEM) was employed. The milled microplastics particle size ranged from 44.2 µm to 552.4 µm. The organ bath technique was employed to study the direct change of tissue contractility of rat isolated tracheal rings. Tracheal rings were incubated with polyethylene microplastics of different concentrations (0.3 mg/ml to 10 mg/ml) for a minimum of 18 hours in physiological Krebs buffer, followed by the construction of concentration-response curves to a contractile agent, carbachol (muscarinic agonist). Exposure to all concentrations of polyethylene microplastics enhanced the contractile responses of the tissues to carbachol. However, the effect was only statistically significant in tissues incubated at 3 mg/ml and above (p < 0.05). Findings from this study provide preliminary evidence that exposure to polyethylene microplastics adversely affects airway function. Heightened contractile responses of airways mimic the pathophysiological responses in respiratory diseases such as asthma, chronic cough and chronic obstructive pulmonary disease. Further experiments focusing on the possible mechanism of actions of these microplastics affecting the airway tissue function are now needed.
近年来,人体通过吸入接触微塑料已被广泛报道。关于微塑料在气道中积聚的直接影响及其如何影响呼吸功能的研究很少。本研究旨在探讨微塑料暴露是否会改变离体气道平滑肌组织的收缩性。采用离心磨对高密度聚乙烯(HDPE)球团进行研磨制备微塑料。为了确认研磨后的微塑料粒径范围在微塑料的定义范围内,采用场发射扫描电镜(FESEM)进行了分析。研磨后的微塑料粒径范围为44.2µm ~ 552.4µm。采用器官浴技术研究大鼠离体气管环组织收缩性的直接变化。气管环与不同浓度的聚乙烯微塑料(0.3 mg/ml至10 mg/ml)在生理克雷布斯缓冲液中孵育至少18小时,然后构建对收缩剂卡巴卡醇(毒蕈碱激动剂)的浓度-响应曲线。暴露于所有浓度的聚乙烯微塑料都增强了组织对苯酚的收缩反应。然而,只有在3 mg/ml及以上孵育的组织中,效果才有统计学意义(p < 0.05)。本研究的发现提供了初步证据,表明接触聚乙烯微塑料会对气道功能产生不利影响。气道收缩反应的增强与哮喘、慢性咳嗽和慢性阻塞性肺疾病等呼吸系统疾病的病理生理反应相似。目前还需要进一步的实验来研究这些微塑料影响气道组织功能的可能机制。
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引用次数: 0
A polyphenol, pyrogallol changes the acidic pH of the digestive vacuole of Plasmodium falciparum 邻苯三酚是一种多酚,能改变恶性疟原虫消化液泡的酸性pH值
Pub Date : 2021-08-02 DOI: 10.28916/lsmb.5.1.2021.82
Nur Saidatul Aqilah Ja’afar, Nik Nor Imam Nik Mat Zin, F. S. Mohamad, N. Abu-Bakar
Pyrogallol has a capability of generating free radicals like other antimalarial drugs such as artemisinin, which is thought to inhibit the proton pump located in the membrane of the Plasmodium falciparum digestive vacuole, thus alkalinising this acidic organelle. This study aimed to determine pH changes of the malaria parasite’s digestive vacuole following treatment with pyrogallol. The antimalarial activity of this compound was evaluated by a malarial SYBR Green 1 fluorescence-based assay to determine the 50% inhibitory concentration (IC50). Based on the IC50 value, different concentrations of pyrogallol were selected to ensure changes of the digestive vacuole pH were not due to parasite death. This was measured by flow cytometry after 4-hour pyrogallol treatment on the fluorescein isothiocyanate-dextran-accumulated digestive vacuole of the mid-trophozoite stage parasites. Pyrogallol showed a moderate antimalarial activity with the IC50 of 2.84 ± 9.40 µM. The treatment of 1.42, 2.84 and 5.67 µM pyrogallol increased 2.9, 3.0 and 3.1 units of the digestive vacuole pH, respectively as compared with the untreated parasite (pH 5.6 ± 0.78). The proton pump, V-type H+-ATPase might be inhibited by pyrogallol, hence causing the digestive vacuole pH alteration, which is similar with the result shown by a standard V-type H+-ATPase inhibitor, concanamycin A. This study provides a fundamental understanding on the antimalarial activity and mechanism of action of pyrogallol that has a potential to be the antimalarial drug candidate.
邻苯三酚具有像其他抗疟疾药物(如青蒿素)一样产生自由基的能力,人们认为青蒿素可以抑制恶性疟原虫消化液泡膜上的质子泵,从而使这种酸性细胞器碱化。本研究旨在测定邻苯三酚治疗后疟原虫消化液泡pH值的变化。该化合物的抗疟活性通过疟疾SYBR Green 1荧光法测定50%抑制浓度(IC50)来评估。根据IC50值,选择不同浓度的邻苯三酚,以确保消化液泡pH的变化不是由寄生虫死亡引起的。这是在邻苯三酚对滋养体中期寄生虫的异硫氰酸葡聚糖积累的荧光素消化液泡处理4小时后用流式细胞术测量的。邻苯三酚具有中等抗疟活性,IC50为2.84±9.40µM。1.42、2.84和5.67µM邻苯三酚处理较未处理的寄生虫(pH 5.6±0.78)分别增加2.9、3.0和3.1个单位的消化液泡pH。质子泵v型H+- atp酶可能被邻苯三酚抑制,从而引起消化液泡pH的改变,这与标准的v型H+- atp酶抑制剂concanamycin a的结果相似。本研究为邻苯三酚的抗疟活性及其作用机制提供了基础认识,邻苯三酚有可能成为抗疟候选药物。
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引用次数: 3
Achievements and progress beyond the current global pandemic 超越当前全球大流行病的成就和进展
Pub Date : 2021-06-14 DOI: 10.28916/lsmb.5.10.2021.83
Y. Cheah
Life Sciences, Medicine and Biomedicine was established in 2017 as an open access, multidisciplinary, peer-reviewed journal. The journal welcomes submissions in all areas of life sciences, medicine and biomedicine with a selection of relevant biological sciences on a case by case basis. It is expected that the wide scope of the journal will promote multidisciplinary collaboration and attract research papers from diverse scientific settings. To put things into perspective, the first issue was published at the end of 2017, with four issues per year through 2018 and 2019 respectively. In 2020, the publication frequency was changed to a single issue annually, adopting to the continuous publication model practiced by many others. Manuscripts are processed for peer-review, and published as they are accepted throughout the year. This method has helped the editorial office to streamline the editorial process. Accepted articles are prepared for publication more robustly and in a timely manner. Based on this experience, the current model of continuous publication will be maintained. In addition to research and review articles, the journal also accepts submissions for mini reviews, opinion pieces and case reports.For the upcoming Volume 6 Issue 1 which will be accepting articles throughout 2022, the journal will be implementing a number of changes to the submission and editorial process. One area currently being improved is the mechanism to allow authors to submit manuscripts with very minimal formatting. Based on the feedback received from our authors, this will significantly reduce the time taken for new submissions to go through initial quality checks and approval, so that the peer-review process can commence as soon as possible. The new author’s guide will be updated and implemented in September 2021. Taking into account the global pandemic, the article processing charges for accepted manuscripts are waived throughout 2021. Further discussions are currently being held to provide financial assistance for future article processing charges for authors that may require them.Despite the challenging period the world is currently facing, the growth of the journal continues. The journal attributes this achievements to the scholarly contributions of the authors, reviewers and editors. During this similar period, the journal received successful approval for indexing with the Directory of Open Access Journals (DOAJ) and Scilit. It is also indexed in MyJurnal (Citation and Infometric Division of the Ministry of Higher Education, Malaysia). Editorial good practices will continue to be enhanced based on industry standards to ensure the journal attracts high quality scientific work. Preparation is also in progress for indexing with other major indexing organizations, which will assist authors and articles to reach a wider global audience.While the journal was initially established for the Malaysian scientific community, the current focus is for authorship and reade
《生命科学、医学与生物医学》创刊于2017年,是一本开放获取、多学科、同行评议的期刊。本刊欢迎生命科学、医学和生物医学所有领域的投稿,并根据具体情况选择相关的生物科学。预计该杂志的广泛范围将促进多学科合作,并吸引来自不同科学背景的研究论文。从实际情况来看,第一期于2017年底出版,到2018年和2019年分别每年出版四期。从2020年开始,将出版频率改为每年一期,采用了许多国家实行的连续出版模式。稿件经过同行评审,并在全年接受后出版。这种方法帮助编辑部简化了编辑过程。被接受的文章准备得更充分、更及时。根据这一经验,将维持目前的连续出版模式。除了研究和评论文章外,该杂志还接受迷你评论、观点文章和案例报告的投稿。对于即将出版的第6卷第1期(将在2022年全年接受文章),该期刊将对提交和编辑过程进行一些更改。目前正在改进的一个领域是允许作者以最小的格式提交手稿的机制。根据我们从作者那里收到的反馈,这将大大减少新投稿通过初始质量检查和批准的时间,从而使同行评审过程能够尽快开始。新的作者指南将于2021年9月更新并实施。考虑到全球大流行,在2021年全年免除已接受稿件的文章处理费。目前正在进一步讨论为可能需要的作者今后的文章处理费提供财政援助。尽管世界正面临着一个充满挑战的时期,但期刊的增长仍在继续。本刊将这一成就归功于作者、审稿人和编辑的学术贡献。在此期间,该期刊成功获得了开放获取期刊目录(DOAJ)和sciit的索引批准。它也在myjournal(马来西亚高等教育部引文与信息计量部门)中被索引。编辑的良好做法将根据行业标准继续得到加强,以确保该期刊吸引高质量的科学工作。与其他主要索引组织进行索引的准备工作也在进行中,这将有助于作者和文章接触到更广泛的全球读者。虽然该杂志最初是为马来西亚科学界创办的,但目前的重点是在东南亚和亚洲地区尽可能广泛地覆盖作者和读者。考虑到这一点,编辑委员会成员的扩大现在包括来自马来西亚、菲律宾和香港的专家。目前正在探索更多的编辑角色,以补充该杂志的多学科范围,并计划让每个研究领域成为其自己部分的先锋。为了使该杂志能够接触到更广泛的全球科学界,最近成立了由来自新加坡、英国、日本、孟加拉国、中国、伊拉克、菲律宾和马来西亚的专家组成的国际咨询委员会。咨询委员会将为期刊的国际扩张提供当前战略和未来方向的反馈,建立良好的做法,并将提交持续质量改进的年度评估。同样令人兴奋的是,该杂志今年将为由马来西亚临床生物化学家协会与马来西亚生物医学科学协会和马来西亚卫生部科学官员协会合作于2021年8月24日至26日举办的2021年亚洲生物医学研究和实验室医学会议出版一期特刊。
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引用次数: 0
Optimization of Claudin-5 and ICAM-1 protein detection by using capillary-based immunoassay method in human brain endothelial cells 基于毛细管免疫分析法的人脑内皮细胞Claudin-5和ICAM-1蛋白检测优化
Pub Date : 2021-06-09 DOI: 10.28916/lsmb.5.1.2021.80
N. Jufri, Tharsini Salyam, Farah Wahida Ibrahim, M. A. Latif, A. Hamid
Background: Human brain endothelial cells (HBECs) are part of the blood-brain barrier (BBB). BBB acts as a barrier to control the passage of molecules or materials from the blood into the brain.  Identification of specific proteins changes in their expressions that are related to disease state is important in order to understand the disease mechanism involving brain vasculature. To achieve that, the techniques involve in identifying the proteins of interest must be optimized prior to further investigation. Methodology: In this study, identification of Claudin-5 in HBEC lysates was tested using different sample preparation techniques such as; 1) reducing with Dithiothreitol (DTT) and non-reducing conditions; 2) denaturing by heating at 95°C for 5 minutes or 70°C for 20 minutes and 3) protein loading at 3 and 4 µg. The samples were then subjected to an automated capillary-based immunoassay, Jess. Results and Discussion: The results showed that HBEC samples loaded at 4 µg and heated for 5 minutes at 95°C with DTT produced clearer and intense bands for Claudin-5 identification compared to the other set ups. As reducing condition and denaturing by heated at 95°C for 5 minutes conditions demonstrated good results, the conditions were used to identify ICAM-1 expression at different protein loading (3 and 4 µg). The result demonstrated that HBEC samples heated for 5 minutes at 95°C with DTT and loaded at 4 µg produced a good detection for ICAM-1. Conclusion: These optimized conditions could be served as a standard procedure for further identification of Claudin-5 and ICAM-1 proteins in HBEC using a capillary immunoassay instrument.
背景:人脑内皮细胞(HBECs)是血脑屏障(BBB)的一部分。血脑屏障的作用是控制分子或物质从血液进入大脑的通道。鉴定与疾病状态相关的特异性蛋白表达变化对于了解涉及脑血管系统的疾病机制具有重要意义。为了实现这一目标,鉴定感兴趣的蛋白质的技术必须在进一步研究之前进行优化。方法:在本研究中,使用不同的样品制备技术测试了HBEC裂解物中Claudin-5的鉴定;1)用二硫苏糖醇(DTT)和非还原条件还原;2)在95°C下加热5分钟或70°C下加热20分钟使其变性,3)在3和4µg下加载蛋白质。然后样本进行了自动毛细管免疫分析,杰西。结果和讨论:结果表明,与其他设置相比,负载为4µg的HBEC样品在95°C下用DTT加热5分钟可以产生更清晰和强烈的条带,用于Claudin-5鉴定。由于还原条件和95°C加热5分钟的条件均表现出良好的效果,因此使用该条件鉴定了不同蛋白负载(3和4µg)下ICAM-1的表达。结果表明,HBEC样品在95°C下用DTT加热5分钟,加载4µg,可以很好地检测ICAM-1。结论:该优化条件可作为毛细管免疫分析仪进一步鉴定HBEC中Claudin-5和ICAM-1蛋白的标准程序。
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引用次数: 1
Knowledge, attitude and practice towards salt intake and health among non-academic staff of Universiti Kebangsaan Malaysia: A pilot study 马来西亚国民大学非学术工作人员对盐摄入和健康的知识、态度和做法:一项试点研究
Pub Date : 2021-06-03 DOI: 10.28916/lsmb.5.10.2021.75
Munirah Ismail, Tuneesha Regu, Martha James Jimponey, Viola Michael, Fathimah Nestha Mohamed, Rohana Hussin, Arimi Fitri Mat Ludin
Background: Over the years, global salt intake has increased and has resulted in the escalation of cardiovascular diseases and hypertension. The lack of knowledge, as well as poor attitude and practice, are the main reasons that have led to high salt intake. Therefore, this study aims to evaluate the knowledge, attitude and practice (KAP) towards salt intake and health among non-academic staff of the Faculty of Health Sciences (FSK), Universiti Kebangsaan Malaysia (UKM). Methodology: This cross-sectional study was conducted among the non-academic staff of the Faculty of Health Sciences (FSK), Universiti Kebangsaan Malaysia (UKM). The respondents were recruited using stratified random sampling technique with an informed consent. A validated questionnaire to test the knowledge, attitude and practice (KAP) towards salt intake and health by the Ministry of Health Malaysia was converted into a Google form and the link was emailed to 118 participants. A total of 33 responses were obtained for evaluation. The significant values were set as P < 0.05. Results: It was observed that female participants had better attitudes towards salt intake and health compared to males (p = 0.044). Also, individuals with higher education levels had better attitudes towards salt intake and health, compared to those with lower education levels (p=0.009). However, knowledge and practice of salt intake and health bore no significant results when considering gender, education level and age. Discussion and Conclusion: Overall, this study has identified sociodemographic characteristics as contributing factors towards individuals’ attitude on salt intake and health. This may provide a crucial foundation in the development of cost-effective healthcare programs to widen knowledge, create awareness and promote a healthy lifestyle by reducing salt intake and further reduce the prevalence of non-communicable diseases.
背景:多年来,全球盐摄入量增加,导致心血管疾病和高血压的升级。知识的缺乏,以及不良的态度和做法,是导致高盐摄入量的主要原因。因此,本研究旨在评估马来西亚国民大学(UKM)健康科学学院(FSK)非学术人员对盐摄入量和健康的知识,态度和实践(KAP)。方法:本横断面研究在马来西亚国民大学(UKM)健康科学学院(FSK)的非学术人员中进行。在知情同意的情况下,采用分层随机抽样技术招募受访者。马来西亚卫生部为测试对盐摄入和健康的知识、态度和做法(KAP)而进行的有效问卷被转换成谷歌表格,并通过电子邮件将链接发送给118名参与者。共获得33份回复进行评估。P < 0.05为显著值。结果:与男性相比,女性对盐摄入和健康的态度更好(p = 0.044)。此外,与受教育程度较低的人相比,受教育程度较高的人对盐摄入和健康的态度更好(p=0.009)。然而,考虑到性别、教育程度和年龄,盐摄入量与健康的知识和实践没有显著的结果。讨论与结论:总体而言,本研究确定了社会人口学特征是影响个人对盐摄入和健康态度的因素。这可能为制定具有成本效益的医疗保健方案提供重要基础,以通过减少盐摄入量来扩大知识、提高认识和促进健康的生活方式,并进一步减少非传染性疾病的流行。
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引用次数: 0
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Life Sciences, Medicine and Biomedicine
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