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Antibacterial and antioxidant activities of various extracts and essential oil from dried leaves of Artemisia herba-alba Asso of Tamanrasset (South Algeria) 南阿尔及利亚塔曼拉塞特(Tamanrasset)青蒿(Artemisia herba-alba Asso)干叶提取物和精油的抑菌和抗氧化活性
Pub Date : 2022-06-03 DOI: 10.53552/ijmfmap.8.1.2022.47-55
Dalila Almi, Hillal Sebbane, Souad Lahcene, Fatma Habera, Kenza Laoudi, A. Mati
The objective of this study was to evaluate the antibacterial and antioxidant activities of two extracts (aqueous and ethanolic) and essential oil from dried sheets of Artemisia herba alba collected in southern Algeria. The extracts were prepared separately with different polarity solvents (water and ethanol). Total phenolics, flavonoids and tanins contents were evaluated. The essential oil was isolated using hydrodistillation. Two tests were established to assess the antioxidant activity (DPPH and FRAP), agar-well diffusion method was used to evaluate the antibacterial effect: Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and Bacillus cereus. The yield of the aqueous extract is higher than that of the ethanolic extract. The phytochemical study revealed the presence of phenolic compounds, flavonoids and tannins. The aqueous extract contains higher amounts of total phenolics (97.17 ± 1.06 mg/g DM), flavonoids (35.61 ± 0.39 mg/g DM) and tannins (46.58 ± 0.91 mg/g DM) compared to the ethanolic extract, 28.69 ± 0.99, 10.98 ± 0.64 and 15.11 ± 0.49 mg/g DM respectively. Antioxidant activity (IC50) of aqueous, ethanolic extracts and essential oil were 2.02, 0.753 and 1.088 mg/ml, respectively. Analysis of the antibacterial activity showed that aqueous extract exhibited much higher activity that the ethanolic extract and essential oil. RP HPLC analysis of aqueous extract show the presence of certain compounds that belong to flavonoids (cathecine and apigenin) and others to phenolic acids (caffeic acid and ferulic acid). The results of this study demonstrated that the essential oil and extracts can be used as antioxidant and antimicrobial agents.
本研究的目的是评价两种提取液(水提液和醇提液)和精油的抑菌和抗氧化活性。分别用不同极性溶剂(水和乙醇)制备提取物。测定了总酚、总黄酮和单宁的含量。采用加氢蒸馏法分离精油。建立抗氧化活性测定法(DPPH和FRAP)和琼脂孔扩散法(琼脂孔扩散法),对大肠杆菌、粪肠球菌、金黄色葡萄球菌和蜡样芽孢杆菌进行抑菌效果测定。水提物得率高于乙醇提物得率。植物化学研究揭示了酚类化合物、类黄酮和单宁的存在。水提物的总酚(97.17±1.06 mg/g DM)、总黄酮(35.61±0.39 mg/g DM)和单宁(46.58±0.91 mg/g DM)含量分别高于乙醇提物(28.69±0.99、10.98±0.64和15.11±0.49 mg/g DM)。水提液、醇提液和精油的抗氧化活性(IC50)分别为2.02、0.753和1.088 mg/ml。抑菌活性分析表明,水提物抑菌活性明显高于醇提物和精油。水提取物的反相高效液相色谱分析显示,某些化合物属于类黄酮(儿茶素和芹菜素),其他化合物属于酚酸(咖啡酸和阿魏酸)。本研究结果表明,其精油和提取物可作为抗氧化和抗菌药物。
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引用次数: 0
Biological valorization and characterization of essential oil of Algerian Mentha spicata L. 阿尔及利亚薄荷精油的生物学鉴定与特性研究。
Pub Date : 2022-06-03 DOI: 10.53552/ijmfmap.8.1.2022.56-60
B. Amel, Medjaher Souhila, D. Mahmoud
Main purpose of our work is to valorise Mentha spicata from the arid zone in Algeria by the study of it biological activities and chemical profile; antibacterial activity and antioxidant activity. Essential oil is characterized by GCMS. Fortyfour constituents, accounting for 98.41% of the total oil contents identified were carbon (42.23%) followed respectively by limonene (29.57%), 1,8-cineole (5.31%), β-pinene (3.54%). The antioxidant activity of the hydro distilled oil was studied using DPPH to determined IC50. The antibacterial activity of essential oils was tested against five microorganisms with the diffusion disc method on bacteria: Staphylococcus aureus ATCC 25923,Candida Albicans ATCC 10231, Escherichia coli ATCC 25922, Bacillus ATCC 11778, Pseudomonas aeruginosa ATCC 27853), diameters vary between 6 and 18 mm.
我们工作的主要目的是通过研究阿尔及利亚干旱地区的薄荷的生物活性和化学特征来评估它的价值;抗菌活性和抗氧化活性。精油用GCMS表征。碳(42.23%)占总油含量的98.41%,其次为柠檬烯(29.57%)、1,8-桉叶油素(5.31%)、β-蒎烯(3.54%)。采用DPPH法测定了氢蒸馏油的IC50,研究了氢蒸馏油的抗氧化活性。采用扩散盘法测定精油对5种细菌的抑菌活性:金黄色葡萄球菌ATCC 25923、白色念珠菌ATCC 10231、大肠杆菌ATCC 25922、芽孢杆菌ATCC 11778、铜绿假单胞菌ATCC 27853,直径在6 ~ 18 mm之间。
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引用次数: 0
A comparative study of maturity indices (heat unit) for indigenous and exotic date palm germplasm at Kachchh-India 印度kachchh地区本地和外来枣椰树种质成熟度指数(热单位)的比较研究
Pub Date : 2022-06-03 DOI: 10.53552/ijmfmap.8.1.2022.27-29
Kapil Mohan Sharma, C. Muralidharan, D. A. Baidiyavadra
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引用次数: 0
Intergeneric and interspecific crossing in Vitaceae: an attempt for disease resistant types Vitaceae属间和种间杂交:抗病类型的尝试
Pub Date : 2022-06-03 DOI: 10.53552/ijmfmap.8.1.2022.12-20
Sujata Tetali, S. Karkamkar, S. Phalake
Wild grapes species are conserved, characterized and utilized at MACS-Agharkar Research Institute, Pune, in a crop improvement programme. In the present study, 20 species/ cultivars belonging to the family Vitaceae collected from different sources within India and abroad were used for making intergeneric as well as interspecific crosses. Out of 263 seeds obtained from 21 cross combinations, four hybrids were successfully grown from V. rotundifolia as one of the parents showed resistance against downy and powdery mildew. The hybrids were evaluated for eleven different fruit characters. These hybrids could be utilized as pre-breeding material in future hybridization programmes.
在浦那MACS-Agharkar研究所的作物改良计划中,野生葡萄品种得到了保护、鉴定和利用。在本研究中,我们利用从印度和国外不同来源收集的20个属/栽培品种进行了属间和种间杂交。在21个杂交组合的263个种子中,由于其中一个亲本对霜霉病和白粉病表现出抗性,成功地培育出4个杂交种。对杂种进行了11个不同的果实性状评价。这些杂交种可作为未来杂交计划的预育种材料。
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引用次数: 0
Comparative accounts on proximate and phytochemical compositions and antioxidant properties of Garcinia quaesita and Garcinia zeylanica. 槲皮藤黄和白花藤黄的化学成分及抗氧化性能比较。
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii02.005
S. Kokilananthan, V. Bulugahapitiya, C. S. Gangabadage, H. Manawadu
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引用次数: 3
Antimitotic and genotoxic effect on the meristematic cells of Allium cepa L. of the alkaloid and flavonoid fractions of the leaves of Peganum harmala L. from the Laghouat region, Algeria. 阿尔及利亚Laghouat地区骆驼蓬(Peganum harmala L.)叶片生物碱和黄酮类成分对葱分生组织细胞的抗有丝分裂和遗传毒性作用。
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii02.008
Y. Ouzid, Ghenima Aiche-Iratni, Lina Harchaoui, Lydia Moussaoui, Lynda Chabane, N. Smail-Saadoun, K. Houali
Medicinal plants are an inexhaustible source of secondary metabolites, namely polyphenols including flavonoids, alkaloids and terpenoids, which generate various biological activities. Keeping this in mind, we were interested in a spontaneous plant Peganum harmala L. to demonstrate the antimitotic and genotoxic effect of the alkaloid and flavonoid fractions (aqueous and butanolic) of the leaves of this species on root meristematic cells of Allium cepa L. Mitotic indices, phase indices, aberration indices as well as cytotoxicity limit values were calculated for our different samples and controls, namely the negative (distilled water) and positive controls (colchicine 1mg/ml and quercetin 1mg/ml). The results indicate a mitodepressant and sublethal effect, observed with the alkaloid and flavonoid fractions of the leaves of Peganum harmala L. Exposure of meristematic cells to the samples resulted in an antimitotic and genotoxic effect translated into a large number of chromosomal, nuclear and cellular aberrations.
药用植物是次生代谢物的取之不尽的来源,即黄酮类、生物碱、萜类等多酚类物质,产生各种生物活性。考虑到这一点,我们对一种自发植物Peganum harmala L.感兴趣,以证明该物种叶片的生物碱和类黄酮组分(水和丁醇)对Allium cepa L.根分生细胞的抗有丝分裂和遗传毒性作用。计算了不同样品和对照的有丝分裂指数、相指数、畸变指数以及细胞毒性极限值。即阴性(蒸馏水)和阳性对照(秋水仙碱1mg/ml和槲皮素1mg/ml)。结果表明,在分生组织细胞暴露于这些样品中,会产生抗有丝分裂和遗传毒性作用,导致大量的染色体、核和细胞畸变。
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引用次数: 1
Evaluation of postharvest quality and shelf life of rose apple (Syzygium jambos) during storage 玫瑰苹果(Syzygium jambos)采后品质及贮藏期评价
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii02.004
Shamima Nashrin, M. Hossain, M. A. Rahim
Three rose apple accessions and five postharvest bagging treatments viz., Control (non-bag), unperforated low density polyethylene (LDPE) bag, perforated LDPE bag, unperforated brown paper bag, and perforated brown paper bag were evaluated for storage quality and shelf life of rose apple. Results showed that different postharvest treatments significantly influenced fruit quality and shelf life of rose apple. Changes in skin colour, weight loss, moisture, dry matter, vitamin C and pH of LDPE bagged fruits irrespective of perforation were slower than nonbagged control fruits during storage at ambient condition. The shelf life of fruit was counted until two weeks of storage. It was found that fruits stored in unperforated LDPE bag exhibited the longest shelf life (14.40 days) as compared to other treatments. The overall results expressed that rose apple quality can be maintained effectively at least six days using various bagging materials. However, it may conclude that unperforated LDPE bag could be used for extending storage life with maintaining external and internal qualities of rose apple.
对3份玫瑰苹果材料和5种采后装袋处理,即对照(无袋)、无孔低密度聚乙烯(LDPE)袋、有孔LDPE袋、无孔牛皮纸袋和有孔牛皮纸袋,对玫瑰苹果的贮藏品质和货架期进行了评价。结果表明,不同采后处理对玫瑰苹果果实品质和货架期有显著影响。环境条件下,无论穿孔与否,LDPE袋装水果的果皮颜色、失重、水分、干物质、维生素C和pH值的变化都比未袋装对照水果慢。水果的保质期被计算到两周。结果表明,无孔LDPE袋贮藏的果实贮藏期最长,达14.40天。综合结果表明,采用不同的套袋材料,月季苹果的品质至少可以保持6天以上。结果表明,无孔LDPE袋可以在保持玫瑰苹果内外品质的前提下延长其贮藏寿命。
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引用次数: 1
Vegetative propagation of Acalypha hispidathrough cuttings with different types of media 不同培养基对木耳扦插无性繁殖的影响
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii02.009
L. Rifnas, N. Vidanapathirana, T. Silva, N. Dahanayake, S. Subasinghe, S. Weerasinghe, W.P.S. Madushani, S. Nelka, H. Rohanadheera
Acalypha hispida is a wild flowering herbaceous plant commonly called as Red-hot cat’s tail or Monkey tail, belongs to family Euphorbiaceae. It is commonly propagated by stem cuttings, but successful propagation of Acalypha hispida by stem cutting is harder, and even the basic information on these aspects are limited. Hence, an experiment was conducted to determine the effects of stem cutting types and growing media on the successful propagation of Acalypha hispida. Four types of stem cuttings (shoot tip, soft wood, semi hardwood and hardwood) and three types of media (pure sand, pure coir dust and 1:1 mixture of sand: coir dust v:v) were used, and the experiment was laid out in Complete Randomized Design (CRD) with 3×4 factor factorial arrangement. Data was analyzed by SAS 9.1.3 software. It was observed that there was a significant (P>0.05) interaction between the cutting types and media used in all the tested parameters except survival rate. All the type of cuttings planted in sand with coir dust showed highest percentage of rooted shoots with well formed root system. Hardwood cuttings planted in pure coir dust and 1:1 ratio of sand: coir dust showed not significant virtuous root weight (0.15g, 0.16g), number of leaves (13.10, 13.43), and shoot length (3.53 cm , 3.34 cm). Cutting types showed significant (P>0.05) in survival and hardwood cuttings showed highest survival rate (80.56%) compared to others. Accordingly, it could be concluded that hardwood cutting as the best material planted in a 1:1 mixture of sand with coir dust and pure coir dust medium can be used to propagate Acalypha hispida plants successfully.
灰菖蒲是一种野生开花草本植物,俗称红猫尾或猴尾,属于大戟科。它通常是通过茎插繁殖,但通过茎插繁殖比较困难,甚至在这些方面的基本信息也很有限。为此,通过试验研究了茎切方式和生长介质对灰果成功繁殖的影响。采用4种茎条(茎尖、软材、半硬木和硬木)和3种介质(纯砂、纯椰砂和1:1混合椰砂v:v),采用完全随机设计(CRD), 3×4因子因子排列。数据分析采用SAS 9.1.3软件。除成活率外,各试验参数与切割类型之间存在显著的交互作用(P>0.05)。不同类型的插枝均表现出根系发育良好的生根芽率最高。在纯椰砂和1:1比例椰砂中种植的硬木插枝,根重(0.15g、0.16g)、叶数(13.10、13.43)、枝长(3.53 cm、3.34 cm)均不显著改善。不同扦插方式对成活率有显著影响(P < 0.05),以硬木扦插方式成活率最高(80.56%)。综上所述,以硬木切块为最佳材料,种植在砂与椰子粉和纯椰子粉1:1的混合培养基中,可成功繁殖木耳。
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引用次数: 0
Momordica dioica Roxb (Spine Gourd)- An underutilized vegetable and medicinal plant in Sri Lanka 苦瓜-斯里兰卡一种未充分利用的蔬菜和药用植物
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii02.011
M.H.G. Weerasinghe, N. Dahanayake
Momordica dioica Roxb belongs to the family Cucurbitaceaeis a wild, perennial and creeping plant growing in the tropical and subtropical countries; Pakistan, China, India, Nepal and Sri Lanka. It is well known as folk medicine and nutritious vegetable as it contains various nutrients and phyto-chemicals with huge medicinal value. Different plant parts of M. dioica provide a number of phyto-constituents; alkaloids, steroids, triterpenoids, flavonoids, glycosides, ursolic acid, vitamins, minerals and rich in fibre. They are potential to cure asthma, excessive salivation and inflammation caused by lizards, snake bite, elephantiasis, fever, mental and digestive disorders and maintain skin health. According to the indigenous knowledge spine gourd plays a vital role in reducing various disorders like urinary disorders, digestive disease and as a remedy for bleeding. At the present scenario, M. dioica is considered as an underutilized, non-neglected plant in Sri Lanka. Extensive identification of M. dioica and its phyto-constituents provide huge potential to protect the plant biodiversity and enhance medicinal utilization. The paper highlighted the nutrient content, different uses, botany and cultivation aspects of M. dioica which will be helpful for the students and the interested peoples.
苦瓜(Momordica dioica Roxb)属于葫芦科,是一种生长在热带和亚热带国家的野生多年生匍匐植物;巴基斯坦、中国、印度、尼泊尔和斯里兰卡。它含有多种营养成分和植物化学物质,具有巨大的药用价值,被誉为民间药材和营养蔬菜。薯蓣的不同植物部位提供多种植物成分;生物碱、类固醇、三萜、黄酮类、糖苷、熊果酸、维生素、矿物质和丰富的纤维。对治疗因蜥蜴、蛇咬、象皮病、发烧、精神和消化系统疾病等引起的哮喘、流涎过多和炎症,以及保持皮肤健康具有潜在的疗效。根据土著知识,葫芦在减少各种疾病,如泌尿系统疾病,消化系统疾病和治疗出血方面起着至关重要的作用。在目前的情况下,杜鹃花在斯里兰卡被认为是一种未被充分利用的、未被忽视的植物。薯蓣及其植物成分的广泛鉴定为保护植物生物多样性和提高药用价值提供了巨大的潜力。本文着重介绍了薯蓣的营养成分、不同用途、植物学和栽培方面的情况,以期对学生和感兴趣的人士有所帮助。
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引用次数: 1
Observations on relationship of seed content with russeting and fruit physical characteristics of apple cv. Golden Delicious 苹果种子含量与赤霉病及果实物理性状关系的观察。金冠苹果
Pub Date : 2021-12-02 DOI: 10.53552/ijmfmap.2021.v07ii01.010
Munib ur Rehman, Mohd Maqbool Mir, Gh. Hassan Rather, E. Parray, Tajamul Nissar, T. Angmo
Apple (Malus x domestica Borkh.) seeds during their development in the fruit, produce a sequence of different types of hormones,influencing fruit growth and quality in diverse ways.This way role of seeds can also be postulated in fruit russeting of apple. So this study was framed to identify the relationship of fruit seed content with qualitative factors like length, diameter and weight,and also russeting incidence in apple cv. ‘Golden Delicious’. Though seed content of the fruit seemed to influence significantly all the above factors, but could only explain the variability in russeting up to 29%, which indicates that there may be many more factors associated with fruit russeting of ‘Golden Delicious’ apple. The study indicated that weight, length and fruit diameter were relatively strongly related to the seed content explaining the variability of 43%, 45% and 46% respectively. Moreover, russeting also influenced negatively the fruit size factors in association with seed number.
苹果(Malus x domestica Borkh.)种子在果实发育过程中产生一系列不同类型的激素,以不同的方式影响果实的生长和品质。这种方式的作用也可以假定种子在果实赤褐色的苹果。因此,本研究旨在确定果实种子含量与长、径、重等定性因子及赤霉病发生率之间的关系。“金冠苹果”。虽然果实的种子含量似乎对上述所有因素都有显著影响,但只能解释高达29%的赤褐色变异,这表明可能有更多的因素与金冠苹果果实赤褐色有关。结果表明,重量、长度和果径与种子含量的相关性较强,其变异率分别为43%、45%和46%。此外,赤褐色对果实大小因子和种子数也有负相关影响。
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引用次数: 0
期刊
International Journal of Minor Fruits, Medicinal and Aromatic Plants
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