Microvascular research最新文献

{"title":"Assessing coronary microvascular dysfunction in refractory no-reflow: Insights from dynamic myocardial perfusion scintigraphy and cardiac MRI","authors":"Stanislav Dil,&nbsp;Vyacheslav Ryabov,&nbsp;Leonid Maslov,&nbsp;Olga Mochula,&nbsp;Andrey Mochula,&nbsp;Maria Kercheva,&nbsp;Konstantin Zavadovsky,&nbsp;Evgeny Vyshlov","doi":"10.1016/j.mvr.2025.104862","DOIUrl":"10.1016/j.mvr.2025.104862","url":null,"abstract":"<div><h3>Background</h3><div>Refractory no-reflow correlates with worse outcomes, including larger infarct sizes, impaired ventricular function, and higher mortality rates, despite advances in percutaneous coronary intervention (PCI). Microvascular obstruction (MVO) and increased left ventricular end-diastolic pressure (LVEDP) are implicated in the pathogenesis, potentially exacerbating ischemic injury and limiting myocardial recovery. While pressure-wire–derived indices such as the Index of Microcirculatory Resistance (IMR) have been validated against MRI-defined MVO in STEMI populations, their invasive nature and procedural complexity limit broad adoption. In contrast, combining dynamic SPECT and cardiac MRI enables a comprehensive non-invasive functional-structural evaluation of coronary microvascular function in refractory no-reflow.</div></div><div><h3>Methods</h3><div>This study is a post hoc analysis of a larger randomized controlled trial (RCT) evaluating the efficacy and safety of intracoronary epinephrine in patients with refractory no-reflow post-PCI (<span><span>ClinicalTrials.gov</span><svg><path></path></svg></span> <span><span>NCT04573751</span><svg><path></path></svg></span>). We evaluated global coronary flow metrics (RMBF, SMBF, gRFI) derived from SPECT and assessed structural markers of microvascular injury (infarct size, MVO) on MRI. Echocardiographic estimations of LVEDP were also analyzed.</div></div><div><h3>Results</h3><div>Dynamic SPECT revealed suboptimal stress myocardial blood flow in most patients, highlighting microvascular impairment. Elevated estimated LVEDP was significantly correlated with indexed MVO (rs = 0.678, <em>p</em> = 0.001). Traditional flow reserve metrics showed limited sensitivity, whereas global relative flow increase (gRFI) showed a statistically significant correlation with MVO, highlighting its added value in detecting stress-induced perfusion abnormalities. Given the small sample and potential outlier influence, this observation should be considered hypothesis-generating.</div></div><div><h3>Conclusion</h3><div>Our findings support that functional impairments—particularly elevated LVEDP and reduced gRFI—are associated with refractory no-reflow. In particular, gRFI may serve as a promising non-invasive marker of microvascular dysfunction, complementing structural imaging. None-theless, further validation in larger cohorts is needed. This study advocates for refined multimodal imaging strategies and tailored therapeutic approaches targeting dynamic microvascular disturbances to improve outcomes in refractory no-reflow.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104862"},"PeriodicalIF":2.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinal blood flow in eyes with primary open-angle glaucoma using a new Adaptive Optics Laser Doppler Velocimeter device","authors":"Ohud Altuwaym ,&nbsp;Martial Geiser ,&nbsp;Thibaud Mautuit ,&nbsp;Frédéric Truffer ,&nbsp;Christophe Chiquet","doi":"10.1016/j.mvr.2025.104858","DOIUrl":"10.1016/j.mvr.2025.104858","url":null,"abstract":"<div><h3>Purpose</h3><div>To measure retinal blood flow (RBF) in the retinal veins of patients with primary open-angle glaucoma (POAG) and to compare it with healthy controls. A secondary objective was to determine any correlation between RBF and visual field (VF) loss or retinal nerve-fiber layer (RNFL) thickness.</div></div><div><h3>Method</h3><div>Twelve patients with POAG and 11 healthy controls were included in a prospective single-center study. Our prototype Adaptive Optics Laser Doppler Velocimeter (AO-LDV) consisted of a laser Doppler velocimeter combined with an adaptive optic fundus camera (rtx1, Imagine Eyes®) allowing measurement of blood vessel diameter and therefore, calculation of blood flow within the vessel. Blood flow in the superior temporal vein (STV) was compared with that in the inferior temporal vein (ITV). All subjects underwent eye examinations including visual field (Humphrey 24-2 SITA-standard strategy) and measurement of retinal nerve-fiber layer (RNFL) thickness using a Cirrus HD-OCT (optic disc 200 × 200 cube) protocol. Sectoral structure-function relationships were studied in the glaucoma group.</div></div><div><h3>Results</h3><div>The velocity in the STV was lower in the glaucoma group (6.30 ± 1.6 mm/s) compared to the control group (8.6 ± 2.8 mm/s, <em>p</em> = 0.07), with no significant differences in the ITV. There were no significant differences in STV or ITV diameters between the groups. We found no relationship between either STV or ITV retinal blood flow and visual field or RNFL thickness.</div></div><div><h3>Conclusion</h3><div>Our prototype AO-LDV allowed accurate measurement of RBF in patients with glaucoma and showed that RBF was not reduced in the early or moderate stages of glaucoma. These preliminary results should be confirmed in a larger study, especially in late-stage glaucoma.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104858"},"PeriodicalIF":2.7,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent heat stress facilitates the autophagy and apoptosis of the vascular endothelium in spontaneously hypertensive rats via the AMPK/mTOR/ULK1 pathway","authors":"Chunli Yang ,&nbsp;Huijuan Zhao ,&nbsp;Xiaomin Wu ,&nbsp;Wei Tuo ,&nbsp;Ling Hou ,&nbsp;Dahai Chai ,&nbsp;Guanghua Li","doi":"10.1016/j.mvr.2025.104857","DOIUrl":"10.1016/j.mvr.2025.104857","url":null,"abstract":"<div><div>This study examined the autophagy and apoptosis of vascular endothelial cells in spontaneously hypertensive rats (SHRs) under intermittent heat stress and determined whether the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR)/Unc-51 like autophagy activating kinase (ULK1) pathway is involved in autophagy regulation. Wistar-Kyoto (WKY) rats were assigned to control (WKY-CN), intermittent heat stress (WKY-8), and continuous heat stress (WKY-24) groups. SHRs were also assigned to three groups: SHR-CN, SHR-8, and SHR-24. Western blotting assay, immunohistochemical assay, and immunofluorescence assay were performed for observing expression of proteins related to autophagy and apoptosis and the AMPK/mTOR/ULK1 pathway. Vascular endothelial cells underwent autophagy and apoptosis following heat stress, as revealed by high expression of autophagy- and apoptosis-related proteins. Heat stress elevated AMPK and ULK1 expression levels, whereas it decreased mTOR phosphorylation in SHR-8 and SHR-24 groups. Finally, the rats in SHR-8 group were administered an autophagy inducer (rapamycin, Rapa) and inhibitor (3-Methyladenine, 3-MA), respectively, for evaluating autophagy induction and inhibition. Following Rapa administration, LC3-II/LC3-I and Caspase-3 expression levels were elevated in the intermittent heat stress groups as compared to those in the control groups; in contrast, 3-MA attenuated cell death in the intermittent heat stress groups. Overall, this study demonstrated that intermittent heat stress elicits autophagy and apoptosis processes in vascular endothelial cells and that the AMPK/mTOR/ULK1 pathway participates in regulating autophagy and apoptosis.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104857"},"PeriodicalIF":2.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of superficial collecting lymph vessels in normal tissue and lymphedema using video-capillaroscopy","authors":"Chihiro Matsui ,&nbsp;Reiko Tsukuura ,&nbsp;Toko Miyazaki ,&nbsp;Shigeki Ishibashi ,&nbsp;Takakuni Tanaka ,&nbsp;Takayoshi Nishimura ,&nbsp;Go Arai ,&nbsp;Joseph M. Escandón ,&nbsp;Hatan Mortada ,&nbsp;Takumi Yamamoto","doi":"10.1016/j.mvr.2025.104842","DOIUrl":"10.1016/j.mvr.2025.104842","url":null,"abstract":"<div><h3>Introduction</h3><div>Lymphedema is a chronic, progressive disorder characterized by impaired lymphatic transport, tissue swelling, and fibrosis. This study used video-capillaroscopy, a high-resolution imaging technique, to assess superficial collecting lymphatic vessels and their vasa vasorum in patients with lymphedema. By comparing these microvascular structures to those in healthy tissue, we aimed to identify early vascular changes contributing to disease progression.</div></div><div><h3>Methods</h3><div>VC recordings were retrospectively analyzed from 28 limbs in 17 patients with lower extremity lymphedema and 53 lymphatic vessels in 12 cancer patients undergoing reconstructive surgery. In the latter group, observations were performed on normal subcutaneous tissue exposed at the donor site during flap harvest. These areas showed no tumor involvement, regional metastasis, or prior chemotherapy/radiotherapy. Thus, all normal tissue observations were made on untreated, unaffected sites. VCLs were classified into six stages (0–5) based on morphology and flow. Vessel diameter and red blood cell (RBC) velocity were measured. Statistical significance was set at <em>p</em> &lt; 0.05.</div></div><div><h3>Results</h3><div>In normal donor tissue, mean VCL main vessel diameter and RBC velocity were 0.038 ± 0.031 mm and 185 ± 160.5 μm/s. In lymphedema, these values were reduced to 0.033 ± 0.024 mm and 28.3 ± 36.8 μm/s (<em>p</em> &lt; 0.001). VCL Stage 0 showed preserved flow (<em>p</em> = 0.178), while Stages 1–5 exhibited progressive impairment.</div></div><div><h3>Conclusion</h3><div>These findings suggest that early ischemic changes in the vasa vasorum may precede lymphatic dysfunction and fibrosis in lymphedema. Preserving microvascular integrity should be a therapeutic focus, alongside drainage support. Further studies are needed to clarify clinical relevance and optimize treatment strategies.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104842"},"PeriodicalIF":2.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of pericyte-like vessel dilation on RBC flux in an In Vitro microvascular network","authors":"Aurelia Bucciarelli,&nbsp;Dominik Obrist","doi":"10.1016/j.mvr.2025.104846","DOIUrl":"10.1016/j.mvr.2025.104846","url":null,"abstract":"<div><div>Efficient oxygen delivery in the brain relies on a finely tuned balance between vascular architecture and dynamic flow regulation. While red blood cells (RBCs) passively flow through the capillary network, neurovascular coupling ensures that the blood supply adapts to meet the metabolic demands of active neurons. Pericytes, contractile cells embedded in the capillary walls, play a key role in this process by modulating capillary diameter in response to neural signals. While pericytes are believed to enable rapid and localized blood flow regulation, their contributions in time and space remain debated. This study investigates the effects of pericyte-like vessel dilation (i.e., pericyte relaxation) on RBC distribution and flow dynamics using an <em>in vitro</em> microfluidic model. We investigate how pericyte-induced dynamic cross-sectional changes affect RBC distribution and velocity in a capillary network. By employing a programmable pressure pump to simulate gradual variations in capillary diameter, we observed that short-time dilation increased RBC velocity and hematocrit near the dilation site, enhancing localized perfusion. In contrast, prolonged dilation led to a network-wide RBC redistribution minimizing hydraulic resistance, ultimately depleting hematocrit due to the network Fåhræus effect. These findings highlight the dynamic and adaptive nature of capillary blood flow, where sustained localized changes can propagate into systemic effects over time. More broadly, this study provides new insights into the interplay between localized flow regulation and systemic capillary network dynamics, revealing how geometric and dynamic factors govern RBC behavior and perfusion.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104846"},"PeriodicalIF":2.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of shear stress as a potential vasoconduction signal across microvascular networks","authors":"Nien-Wen Hu ,&nbsp;M.M.N. Hossain ,&nbsp;Julia Withrow ,&nbsp;Ryan Walker ,&nbsp;Ali Kazempour ,&nbsp;Nikolaos Tsoukias ,&nbsp;Donald G. Welsh ,&nbsp;Walter L. Murfee ,&nbsp;Peter Balogh","doi":"10.1016/j.mvr.2025.104855","DOIUrl":"10.1016/j.mvr.2025.104855","url":null,"abstract":"<div><div>The objective of this study was to computationally estimate the effects of vessel specific vasoconstriction on immediate shear stress changes across microvascular networks. Shear stress due to microvascular blood flow is an established initiator of ion-mediated signaling along microvessels which regulates control of microcirculatory blood flow. Yet, beyond initiating local vasomotion in a vessel, shear stress as a vasoconduction signal itself and characteristics of hydrodynamic propagation via blood flow are not well understood. In the current work, we use images of mesenteric microvascular networks from adult rat tissues and a network segmental blood flow model to simulate various vessel constriction scenarios and estimate subsequent shear stress changes and distances these changes spread from the site of constriction. Scenarios involving both arteriolar constriction and capillary constriction are considered, in addition to a microvascular network from muscle tissue. The findings generally reveal heterogenous and physiologically relevant shear stress changes across the networks for all cases, with magnitudes spanning a wide range and can exceed 30 dyne/cm². Further, physiological relevant wall shear changes were predicted at distances several mm from the stimulus site. Spatial patterns of shear stress change relative to network topology and capillary density are also identified. Altogether, the results invigorate consideration and discussion about shear stress as a potential player in vasoconduction responses.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104855"},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCSK9 inhibitor improved cardiac function after acute myocardial infarction in rats","authors":"Hongjin An ,&nbsp;Junju Zhu ,&nbsp;Qianqian Li","doi":"10.1016/j.mvr.2025.104847","DOIUrl":"10.1016/j.mvr.2025.104847","url":null,"abstract":"<div><h3>Background</h3><div>This study investigated the effects and possible mechanisms of Protein expression of protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor evolocumab on cardiac function in rats with acute myocardial infarction (AMI).</div></div><div><h3>Methods</h3><div>The AMI model was established by ligating the left anterior descending coronary artery in rats. The mRNA expression of PCSK9 in myocardial tissues was detected by real-time fluorescent quantitative PCR (RT-qPCR). Echocardiography was used to examine the cardiac function indexes. Hematoxylin-eosin (HE) and Masson stainings were used to detect myocardial pathologic injury. 2,3,5-Triphenyltetrazolium chloride (TTC) staining was used to detect myocardial infarction area. Serum levels of low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), lactate dehydrogenase (LDH), creatine kinase isoenzymes (CK-MB), cardiac troponin T (cTnT), interleukin-1β (IL-1β), interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) were detected by ELISA. CD31 and vascular endothelial growth factor (VEGF) positive expression was detected by immunohistochemistry. Protein expression levels of PCSK9, Bax, Bcl-2, cleaved-caspase3, receptor interacting protein kinase 1 (RIPK1), RIPK3, mixed lineage kinase domain-like (MLKL), and p-MLKL were detected in myocardial tissues by western blot.</div></div><div><h3>Results</h3><div>The mRNA level and protein expression of PCSK9 were significantly increased in AMI rats. PCSK9 inhibitor evolocumab reduced the levels of LDL-C and TC in serum, thereby improving dyslipidemia. And, evolocumab up-regulated the levels of left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS), and down-regulated the levels of left ventricular end systolic diameter (LVESD), left ventricular end diastolic diameter (LVEDD), left ventricular end systolic volume (LVESV), and left ventricular end diastolic volume (LVEDV), which in turn improved cardiac function. In addition, evolocumab attenuated myocardial pathological injury, reduced myocardial infarction area, lowered the levels of LDH, CK-MB, cTnT, IL-1β, IL-17, and TNF-α, and inhibited apoptosis rate, down-regulated Bax, cleaved-caspase3, RIPK1, RIPK3, MLKL, p-MLKL expression, and up-regulated Bcl-2 protein expression, CD31 and VEGF positive expression.</div></div><div><h3>Conclusion</h3><div>The PCSK 9 inhibitor evolocumab improved cardiac function in AMI rats, and the mechanism may be related to the RIPK1/RIPK3/MLKL pathway.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"162 ","pages":"Article 104847"},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel in vivo porcine models of chronic ischemic tissue","authors":"Veronika Frelichova ,&nbsp;Robert Bem ,&nbsp;Jaroslav Chlupac ,&nbsp;Michal Dubsky ,&nbsp;Jitka Husakova ,&nbsp;Andrea Nemcova ,&nbsp;Ludek Voska ,&nbsp;Zuzana Simunkova ,&nbsp;Filip Tichanek ,&nbsp;Jiri Fronek","doi":"10.1016/j.mvr.2025.104845","DOIUrl":"10.1016/j.mvr.2025.104845","url":null,"abstract":"<div><div>There is a lack of reliable in vivo models that replicate limb-threatening ischemia in humans. To fill this gap, we developed and validated two novel porcine ischemic models: ischemic limb and dorsal flap models, both with and without streptozotocin-induced hyperglycemia (<em>N</em> = 3 per group, 12 in total). Hind limb ischemia model was induced via different arterial ligations, with two ischemic and three control wounds per animal. In the flap model, four full-thickness flaps were created on the dorsum with silicone sheets to block reperfusion, and excisional wounds were made on the top. One non-ischemic wound served as control. Transcutaneous oxygen pressure (TcPO₂), wound area, and microvascular density were measured, with TcPO₂ and wound area assessed longitudinally. Data analysis focused on detailed visualization and Bayesian hierarchical modelling to account for the small sample size. Developed models exhibited stable ischemia and prolonged wound healing, with TcPO₂ remaining under 30 mmHg over 28 days, and wound healing extending beyond two weeks. The flap model showed slower TcPO₂ recovery and greater chronicity compared to the limb model, without reliable effect of hyperglycemia. Thus, the porcine flap model shows the highest potential as a relevant model for chronic limb-threatening ischemia.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104845"},"PeriodicalIF":2.9,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of neovascularization in murine osteoarthritis using micro-computed tomography","authors":"Reza Talaie,&nbsp;Pooya Torkian,&nbsp;Anthony Spano,&nbsp;Alexander Clayton,&nbsp;Jafar Golzarian","doi":"10.1016/j.mvr.2025.104844","DOIUrl":"10.1016/j.mvr.2025.104844","url":null,"abstract":"<div><h3>Purpose</h3><div>The study aimed to examine neovascularization in murine osteoarthritis (OA) using micro-computed tomography (μCT).</div></div><div><h3>Materials and methods</h3><div>OA was induced in eighteen mice through intra-articular collagenase injection, designating the left hindlimbs as OA models and the right hindlimbs as controls. Mice were monitored for 4, 8, or 12 weeks post-induction. Hindlimbs underwent overnight tissue fixation and were then subjected to μCT scanning. Quantification of unnamed arterial branches spanned from the femoral artery's terminal branching point to 2.5 mm below the tibial plateau.</div></div><div><h3>Results</h3><div>Baseline characteristics did not differ significantly between control and OA-induced groups (p &gt; 0.05). Collagenase-treated limbs showed a significantly higher number of unnamed arterial branches compared to controls (11.6 vs. 7.5, p &lt; 0.001), reflecting increased neovascularization. This elevation persisted across all post-induction time points, with no significant time-dependent trend (p = 0.09) or interaction between time and treatment group (p = 0.17). Spatial analysis revealed that neovessels were predominantly localized to peri-meniscal (61 %) and subchondral (29 %) regions.</div></div><div><h3>Conclusion</h3><div>Collagenase-induced OA in mice results in sustained and spatially patterned neovascularization, detectable using non-contrast μCT. These findings underscore the utility of μCT for tracking vascular remodeling in OA and highlight potential anatomical targets for angiogenesis-modulating therapies.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104844"},"PeriodicalIF":2.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The electrophysiological effects of angiotensin 1–7 on hypertrophic myocardium in spontaneously hypertensive rats","authors":"Xin Yu ,&nbsp;Zhen Yang ,&nbsp;Zhilan Ma ,&nbsp;Ru Yan ,&nbsp;Jianzhong Zhang","doi":"10.1016/j.mvr.2025.104843","DOIUrl":"10.1016/j.mvr.2025.104843","url":null,"abstract":"<div><div>This study aimed to explore the effects of Angiotensin 1–7 (Ang1–7) on electrophysiological remodeling of hypertensive hypertrophic myocardium in spontaneously hypertensive rats (SHR). Thirty male SHR rats were equally divided into three groups: SHR control group (SHR<img>C) treated with saline; Ang1–7 group (SHR-A) treated with Ang1–7[25 μg·(kg min)⁻¹] and Ang1–7 blocker group (SHR<img>B) treated with A779 [72 μg·(kg min)⁻¹]. Wistar-Kyoto (WKY) rats (<em>n</em> = 10) were used as a normotensive group. The treatment period was 5 weeks. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured. Echocardiography was used to evaluate cardiac function. The ventricular myocytes were isolated for evaluation of electrophysiological remodeling of myocardium using microelectrode and patch clamp techniques. When compared with those in WKY rats before treatment, systolic and diastolic pressures were significantly higher in SHR (<em>P</em> &lt; 0.01 and <em>P</em> &lt; 0.001 respectively), left ventricular end-diastolic diameter (LVEDd) was significantly lower (<em>P</em> &lt; 0.05), while diastolic interventricular septum thickness (IVSd), diastolic left ventricular posterior wall thickness (LVPWd) were significantly greater in SHR (<em>P</em> &lt; 0.01). After 5 weeks of treatment, compared with SHR-C group, SBP, DBP, IVSd, LVPWd in SHR-A group were lower significantly (<em>P</em> &lt; 0.05), while SBP in SHR-B group was significantly higher (<em>P</em> &lt; 0.05). Compared with WKY group, the resting potential (RP), action potential amplitude (APA) and transient sodium current (<em>I</em>_Na-T) in SHR group were significantly lower (<em>P</em> &lt; 0.05), and the action potential duration (APD₂₀, APD₅₀ and APD₉₀) in SHR group was greater (<em>P</em> &lt; 0.05). After Ang1–7 intervention, RP, APA and <em>I</em>_Na-T were significantly greater in SHR-A than those in SHR-C (<em>P</em> &lt; 0.05), and APD in SHR-A significantly lower than that in SHR-C (<em>P</em> &lt; 0.05). Taken together, these results demonstrated that Ang1–7 can not only decrease the blood pressure, but reverse the myocardial hypertrophy and electrophysiological remodeling as well in SHR rats.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104843"},"PeriodicalIF":2.9,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}