Pub Date : 2021-03-16DOI: 10.15567/MLJEKARSTVO.2021.0201
J. Vitas
Milk-based kombucha beverages were obtained conducting kombucha lead fermentation of milk. In order to discriminate the analysed samples and to detect similarities or dissimilarities among them in the space of experimentally determined variables, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were applied. Linear discriminant analysis (LDA) was conducted on the raw data set in order to find a rule for allocating a new sample of unknown origin to the correct group of samples. In the space of the variables analysed by HCA, the dominant discriminating factor for the studied samples of kombucha beverages is the milk fat (MF) content, followed by total unsaturated fatty acids content (TUFA), monounsaturated fatty acids content (MUFA) and polyunsaturated fatty acids content (PUFA). The samples with 0.8 and 1.6% milk fat belong to the same cluster in the space of the analysed variables due to similarities in their AADPPH. It was determined by LDA that there was the biggest difference in quality between the groups of products with winter savoury and stinging nettle, while the highest similarity is between groups of products with wild thyme and peppermint regarding their pH values and antioxidant activity expressed as AADPPH.
{"title":"Chemometric approach to quality characterization of milk-based kombucha beverages","authors":"J. Vitas","doi":"10.15567/MLJEKARSTVO.2021.0201","DOIUrl":"https://doi.org/10.15567/MLJEKARSTVO.2021.0201","url":null,"abstract":"Milk-based kombucha beverages were obtained conducting kombucha lead fermentation of milk. In order to discriminate the analysed samples and to detect similarities or dissimilarities among them in the space of experimentally determined variables, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were applied. Linear discriminant analysis (LDA) was conducted on the raw data set in order to find a rule for allocating a new sample of unknown origin to the correct group of samples. In the space of the variables analysed by HCA, the dominant discriminating factor for the studied samples of kombucha beverages is the milk fat (MF) content, followed by total unsaturated fatty acids content (TUFA), monounsaturated fatty acids content (MUFA) and polyunsaturated fatty acids content (PUFA). The samples with 0.8 and 1.6% milk fat belong to the same cluster in the space of the analysed variables due to similarities in their AADPPH. It was determined by LDA that there was the biggest difference in quality between the groups of products with winter savoury and stinging nettle, while the highest similarity is between groups of products with wild thyme and peppermint regarding their pH values and antioxidant activity expressed as AADPPH.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":"71 1","pages":"83-94"},"PeriodicalIF":1.2,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44972124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.15567/MLJEKARSTVO.2021.0206
R. Loučka
The aim of this study was to confirm the hypothesis that artificial brushes administered to the rumen can partially replace the function of structural fibre, and increase milk production or quality. To mitigate the risks of feeding low levels of physically effective neutral detergent fibre (peNDF) to cattle, the administration of ruminal mechanical stimulating (RMS) brushes was examined in 22 high-yielding lactating Holstein dairy cows. The cows were divided into an experimental group equipped with RMS brushes and a control group without RMS. Cows were fed four experimental total mixed rations (TMR) consisting of fixed amounts of alfalfa silage, maize silage, rush corn cob mix silage and different proportions of brewer’s grains, concentrate and wheat straw. The TMRs had the following 4 peNDF contents: 10.9 %, 13.0 %, 12.6 % and 14.0 %. The duration of the experiment was 18 weeks. All cows were fed TMRs with a low structural fibre content near levels associated with a risk of subacute ruminal acidosis (SARA). For the RMS brush group, 3 RMS brushes were inserted orally into the rumen using a special applicator. The effects of RMS brushes on feed intake, rumen fermentation and milk production were evaluated. Ruminal fluid (250 mL) was taken using a stomach tube for pH, volatile fatty acids and ammonia nitrogen analysis. A significant increase was found for the feed intake and milk yield of the RMS group fed the TMRs with 13.0 % peNDF although no relationship between peNDF content and RMS TMRs was found. No significant increase in milk quality, rumen pH or rumen fermentation metabolites was declared for the group with RMS brushes. Although RMS brush technology only partially reduced the requirements for peNDF, it may decrease the risk of SARA.
{"title":"Effect of ruminal mechanical stimulating brushes on the performance of lactating Holstein dairy cows","authors":"R. Loučka","doi":"10.15567/MLJEKARSTVO.2021.0206","DOIUrl":"https://doi.org/10.15567/MLJEKARSTVO.2021.0206","url":null,"abstract":"The aim of this study was to confirm the hypothesis that artificial brushes administered to the rumen can partially replace the function of structural fibre, and increase milk production or quality. To mitigate the risks of feeding low levels of physically effective neutral detergent fibre (peNDF) to cattle, the administration of ruminal mechanical stimulating (RMS) brushes was examined in 22 high-yielding lactating Holstein dairy cows. The cows were divided into an experimental group equipped with RMS brushes and a control group without RMS. Cows were fed four experimental total mixed rations (TMR) consisting of fixed amounts of alfalfa silage, maize silage, rush corn cob mix silage and different proportions of brewer’s grains, concentrate and wheat straw. The TMRs had the following 4 peNDF contents: 10.9 %, 13.0 %, 12.6 % and 14.0 %. The duration of the experiment was 18 weeks. All cows were fed TMRs with a low structural fibre content near levels associated with a risk of subacute ruminal acidosis (SARA). For the RMS brush group, 3 RMS brushes were inserted orally into the rumen using a special applicator. The effects of RMS brushes on feed intake, rumen fermentation and milk production were evaluated. Ruminal fluid (250 mL) was taken using a stomach tube for pH, volatile fatty acids and ammonia nitrogen analysis. A significant increase was found for the feed intake and milk yield of the RMS group fed the TMRs with 13.0 % peNDF although no relationship between peNDF content and RMS TMRs was found. No significant increase in milk quality, rumen pH or rumen fermentation metabolites was declared for the group with RMS brushes. Although RMS brush technology only partially reduced the requirements for peNDF, it may decrease the risk of SARA.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41548299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.15567/MLJEKARSTVO.2021.0205
N. Ünver
In this research, Sanliurfa butterfat, collected from local small-sized dairy plants located in Karacadağ Region of Sanliurfa, were stored at two different temperatures (4 and 20 °C) for 12 months. The butterfat samples have been analysed to determine some biochemical parameters (free fatty acids, acid value, peroxide value, induction time, and pH) and fatty acid composition on the 1st, 30th, 90th, 120th, 180th, 270th and 360th days of storage. The mean value of free fatty acids, acid value and peroxide value of the butterfat stored in refrigerator conditions (4 °C) were lower, and the induction time was higher than the butterfat stored at room temperature (20 °C) (P<0.05). Furthermore, the acid value, free fatty acids value, C16:1, C17:1, C18:2n6c and MUFA of the butterfat increased significantly during the storage period (P<0.05). The ratio of saturated fatty acid of the butterfat decreased during the storage (P<0.05). According to the results of free fatty acids, traditional Sanliurfa butterfat at room temperature (20 °C) could be stored safely for a maximum of 3 months, while this period could be up to 6 months for the butterfat stored at refrigerated temperature (4 °C).
{"title":"Storage stability and fatty acid composition of Sanliurfa butterfat","authors":"N. Ünver","doi":"10.15567/MLJEKARSTVO.2021.0205","DOIUrl":"https://doi.org/10.15567/MLJEKARSTVO.2021.0205","url":null,"abstract":"In this research, Sanliurfa butterfat, collected from local small-sized dairy plants located in Karacadağ Region of Sanliurfa, were stored at two different temperatures (4 and 20 °C) for 12 months. The butterfat samples have been analysed to determine some biochemical parameters (free fatty acids, acid value, peroxide value, induction time, and pH) and fatty acid composition on the 1st, 30th, 90th, 120th, 180th, 270th and 360th days of storage. The mean value of free fatty acids, acid value and peroxide value of the butterfat stored in refrigerator conditions (4 °C) were lower, and the induction time was higher than the butterfat stored at room temperature (20 °C) (P<0.05). Furthermore, the acid value, free fatty acids value, C16:1, C17:1, C18:2n6c and MUFA of the butterfat increased significantly during the storage period (P<0.05). The ratio of saturated fatty acid of the butterfat decreased during the storage (P<0.05). According to the results of free fatty acids, traditional Sanliurfa butterfat at room temperature (20 °C) could be stored safely for a maximum of 3 months, while this period could be up to 6 months for the butterfat stored at refrigerated temperature (4 °C).","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":"71 1","pages":"124-131"},"PeriodicalIF":1.2,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46543063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.15567/MLJEKARSTVO.2021.0207
Paweł Solarczyk
The intensive breeding work of Holstein-Friesian cattle has led to the decrease in the diversity within the population and to inbreeding depression, which may impair its functional traits. In addition, as shown by the research, production traits are negatively correlated with functional traits such as reproduction, health, and longevity, which have a very strong impact on the profit of dairy farms. The aim of this study was to compare milk yield traits of hybrids obtained by crossbreeding of Polish Holstein Friesian (PHF) cows and Swedish Red (SRB) bulls with values obtained for pure PHF cows. For the study, 100 primiparous cows were selected and divided into two groups. The experimental group consisted of 50 crossbreds (PHF×SRB), while the control group consisted of 50 purebred PHF cows. The study showed a higher content of milk components (fat by 11.78 %, protein by 9.06 %, dry matter by 5.75 %) in PHF×SRB, as compared to PHF. A lower level of SCC (by 38.94 %) has also been shown in hybrids, which indicates their higher resistance to udder diseases. The experiment demonstrated a highly significant impact of heterosis on performance parameters and technological quality of milk in F1 generation obtained as a result of crossbreeding between PHF cows and SRB bulls.
{"title":"A comparison between Polish Holstein-Friesian and F1 hybrid Polish Holstein Friesian × Swedish Red cows in terms of milk yield traits","authors":"Paweł Solarczyk","doi":"10.15567/MLJEKARSTVO.2021.0207","DOIUrl":"https://doi.org/10.15567/MLJEKARSTVO.2021.0207","url":null,"abstract":"The intensive breeding work of Holstein-Friesian cattle has led to the decrease in the diversity within the population and to inbreeding depression, which may impair its functional traits. In addition, as shown by the research, production traits are negatively correlated with functional traits such as reproduction, health, and longevity, which have a very strong impact on the profit of dairy farms. The aim of this study was to compare milk yield traits of hybrids obtained by crossbreeding of Polish Holstein Friesian (PHF) cows and Swedish Red (SRB) bulls with values obtained for pure PHF cows. For the study, 100 primiparous cows were selected and divided into two groups. The experimental group consisted of 50 crossbreds (PHF×SRB), while the control group consisted of 50 purebred PHF cows. The study showed a higher content of milk components (fat by 11.78 %, protein by 9.06 %, dry matter by 5.75 %) in PHF×SRB, as compared to PHF. A lower level of SCC (by 38.94 %) has also been shown in hybrids, which indicates their higher resistance to udder diseases. The experiment demonstrated a highly significant impact of heterosis on performance parameters and technological quality of milk in F1 generation obtained as a result of crossbreeding between PHF cows and SRB bulls.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":"71 1","pages":"141-150"},"PeriodicalIF":1.2,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45559559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.15567/mljekarstvo.2021.0103
E. Sulejmani
This study aimed to investigate the influence of substitution of milk fat with palm fat on the composition, yield and technological quality of White cheese, a Macedonian traditional cheese. In this study, fullfat white brined cheese was used as a control sample (WC), while experimental cheeses were prepared from cow milk with vegetable fat (WV) addition and low-fat cheese (WL), respectively. The cheeses have been analysed for physicochemical (acidity, pH, fat, fat acidity, protein), instrumental texture and colour properties (L, a, b) as well as the sensory properties after 1, 20 and 60 days. WV cheeses showed a significantly (P<0.05) lower degree of pH and higher titratable acidity than the WC and WL cheese samples. Cheese that contained vegetable fat showed lower degrees of lipolysis, as assessed by the acid-degree value and received significantly (P<0.05) better appearance, highest hardness value (5226.98 N) and cheese yield scores compared to other samples.
{"title":"Effect of vegetable fat on the texture, colour and sensory properties of Macedonian white brined cheese","authors":"E. Sulejmani","doi":"10.15567/mljekarstvo.2021.0103","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2021.0103","url":null,"abstract":"This study aimed to investigate the influence of substitution of milk fat with palm fat on the composition, yield and technological quality of White cheese, a Macedonian traditional cheese. In this study, fullfat white brined cheese was used as a control sample (WC), while experimental cheeses were prepared from cow milk with vegetable fat (WV) addition and low-fat cheese (WL), respectively. The cheeses have been analysed for physicochemical (acidity, pH, fat, fat acidity, protein), instrumental texture and colour properties (L, a, b) as well as the sensory properties after 1, 20 and 60 days. WV cheeses showed a significantly (P<0.05) lower degree of pH and higher titratable acidity than the WC and WL cheese samples. Cheese that contained vegetable fat showed lower degrees of lipolysis, as assessed by the acid-degree value and received significantly (P<0.05) better appearance, highest hardness value (5226.98 N) and cheese yield scores compared to other samples.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45796092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.15567/mljekarstvo.2021.0104
O. Tomar
The aim of this study was to use various plants to increase the functional properties of yoghurt. The ethanol extracts of three different plant (Mentha piperita L., Ocimum basilicum L., and Hibiscus sabdariffa L.) extracts were added to the stirred type yoghurts at different ratios (0.1 %, 0.3 % and 0.5 %). The pH values, colour values, antioxidant activity, microbial and sensory attributes of yoghurt were evaluated on the storage days of 0, 7, 14, 21, and 28. The lowest pH values were found at the beginning (4.29) and at the end (3.95) of the storage period in samples containing 0.5 % hibiscus extract. The addition of plant extracts in amounts of 0.3 % and higher decreased the lightness (L*) value of yoghurt (p<0.05), and the maximum decrease was found in yoghurt samples containing 0.5% hibiscus ethanol extract. The concentrations of added mint and basil extracts increased the yellowness (b*) value (p<0.05), while the addition of hibiscus extract significantly increased the redness value (p<0.05). Hibiscus ethanol extract significantly reduced the growth of total aerobic mesophilic bacteria (TAMB), lactic acid bacteria (LAB) and Lactococcus/Streptococcus bacteria (p<0.05). The antioxidant activity of all samples increased with adding plant extracts. According to the results of sensory analysis, the addition of 0.1 % plant extracts to yoghurts rated higher scores than that of the control sample, but the addition of 0.3 % and 0.5 % ethanol extracts negatively affected the sensory properties. It can be concluded that adding plant extracts had a positive effect on the sensory and functional properties of yoghurt.
{"title":"The effect of plant extracts on antioxidant potential, microbial and sensory attributes of stirred yoghurt","authors":"O. Tomar","doi":"10.15567/mljekarstvo.2021.0104","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2021.0104","url":null,"abstract":"The aim of this study was to use various plants to increase the functional properties of yoghurt. The ethanol extracts of three different plant (Mentha piperita L., Ocimum basilicum L., and Hibiscus sabdariffa L.) extracts were added to the stirred type yoghurts at different ratios (0.1 %, 0.3 % and 0.5 %). The pH values, colour values, antioxidant activity, microbial and sensory attributes of yoghurt were evaluated on the storage days of 0, 7, 14, 21, and 28. The lowest pH values were found at the beginning (4.29) and at the end (3.95) of the storage period in samples containing 0.5 % hibiscus extract. The addition of plant extracts in amounts of 0.3 % and higher decreased the lightness (L*) value of yoghurt (p<0.05), and the maximum decrease was found in yoghurt samples containing 0.5% hibiscus ethanol extract. The concentrations of added mint and basil extracts increased the yellowness (b*) value (p<0.05), while the addition of hibiscus extract significantly increased the redness value (p<0.05). Hibiscus ethanol extract significantly reduced the growth of total aerobic mesophilic bacteria (TAMB), lactic acid bacteria (LAB) and Lactococcus/Streptococcus bacteria (p<0.05). The antioxidant activity of all samples increased with adding plant extracts. According to the results of sensory analysis, the addition of 0.1 % plant extracts to yoghurts rated higher scores than that of the control sample, but the addition of 0.3 % and 0.5 % ethanol extracts negatively affected the sensory properties. It can be concluded that adding plant extracts had a positive effect on the sensory and functional properties of yoghurt.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44406645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.15567/mljekarstvo.2021.0101
A. Ivanković
Over the past three decades, the scientific and public interest has been initiated by studies in which the negative effect of milk consumption of beta-casein A1 variant has been observed on consumers’ health. The production of “A2 milk” is one of the ways to economically increase the competitiveness of small and medium-sized dairy farms. Breeders of endangered local breeds are also interested in reaffirmation through production of “A2 milk”. The aim of this study is to determine frequencies of A1 and A2 variants of beta-casein in three commercial and three local breeds of cattle in Croatia, and their relationship with production indicators of commercial breeds in the first three lactations. The genomic DNA was extracted from hair and determination of CSN2 genotypes was conducted by PCR RFLP method. Data for milk yield and chemical composition were provided by the central database. The dominant presence of A2 variant beta-casein in the investigated cattle breeds (0.650-0.758) and the increase in the frequency of A2 beta-casein in the population of Simmental and Istrian cattle were determined. The association of A2A2 and A1A1 genotypes of beta-casein with lactation production and milk fat content in the first and second lactation was observed (p<0.05). The dominance of the A2 allelic variation of beta-casein makes the researched conventional and local breeds suitable for the production of “A2 milk”. The breeding extension of A2 allelic variation of beta-casein within local cattle breeds should be carried out carefully trying not to lose part of the existing genetic variability.
{"title":"Genetic polymorphism and effect on milk production of CSN2 gene in conventional and local cattle breeds in Croatia","authors":"A. Ivanković","doi":"10.15567/mljekarstvo.2021.0101","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2021.0101","url":null,"abstract":"Over the past three decades, the scientific and public interest has been initiated by studies in which the negative effect of milk consumption of beta-casein A1 variant has been observed on consumers’ health. The production of “A2 milk” is one of the ways to economically increase the competitiveness of small and medium-sized dairy farms. Breeders of endangered local breeds are also interested in reaffirmation through production of “A2 milk”. The aim of this study is to determine frequencies of A1 and A2 variants of beta-casein in three commercial and three local breeds of cattle in Croatia, and their relationship with production indicators of commercial breeds in the first three lactations. The genomic DNA was extracted from hair and determination of CSN2 genotypes was conducted by PCR RFLP method. Data for milk yield and chemical composition were provided by the central database. The dominant presence of A2 variant beta-casein in the investigated cattle breeds (0.650-0.758) and the increase in the frequency of A2 beta-casein in the population of Simmental and Istrian cattle were determined. The association of A2A2 and A1A1 genotypes of beta-casein with lactation production and milk fat content in the first and second lactation was observed (p<0.05). The dominance of the A2 allelic variation of beta-casein makes the researched conventional and local breeds suitable for the production of “A2 milk”. The breeding extension of A2 allelic variation of beta-casein within local cattle breeds should be carried out carefully trying not to lose part of the existing genetic variability.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45852219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.15567/mljekarstvo.2021.0102
Ž. K. Šalavardić, V. Biotechnology
Due to the high content of crude protein and high concentration of unsaturated fatty acids, pumpkin seed cake (PSC) could be a good quality feedstuff for ruminants, while extruded linseed (ELS) has already shown some beneficial effect on fatty acids proportion of goat milk. Besides, lactation is the most demanding period when monitoring the metabolic condition of animal. Therefore, the aim of this study was to determine the effect of PSC and ELS replacing soybean meal in diets of dairy goats on blood haemato-chemical parameters and milk quality. A 28 lactating French Alpine dairy goats were monitored within 20 days in early lactation. Diet of each groups had a balanced content of energy and crude protein, and a slight difference in lipid content. Goats in control group (n = 9) received soybean meal and extruded soybean. In experimental groups soybean was partially replaced with ELS (n = 10) or completely with PSC (n = 9). Dietary treatments had no effect on haemato-chemical parameters, although PSC increased concentration of globulins in serum. PSC lowered linoleic acid compared to control. ELS increased proportion of α-linolenic acid, eicosapentaenoic acid, and total n-3 fatty acids in milk. Positive correlations were found between non-esterified fatty acids (NEFA) and ratio C18:1/C15:0, NEFA and ratio C17:0/C15:0, as well as β-hydroxybutyrate and ratio C17:0/C15:0. The replacement of soybean meal with PSC or partially with ELS is feasible with respect to haemato-chemical parameters of early lactating dairy goats, while ELS had much beneficial effect on fatty acids compared to PSC.
{"title":"The effect of dietary pumpkin seed cake and extruded linseed on blood haemato-chemicals and milk quality in Alpine goats during early lactation","authors":"Ž. K. Šalavardić, V. Biotechnology","doi":"10.15567/mljekarstvo.2021.0102","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2021.0102","url":null,"abstract":"Due to the high content of crude protein and high concentration of unsaturated fatty acids, pumpkin seed cake (PSC) could be a good quality feedstuff for ruminants, while extruded linseed (ELS) has already shown some beneficial effect on fatty acids proportion of goat milk. Besides, lactation is the most demanding period when monitoring the metabolic condition of animal. Therefore, the aim of this study was to determine the effect of PSC and ELS replacing soybean meal in diets of dairy goats on blood haemato-chemical parameters and milk quality. A 28 lactating French Alpine dairy goats were monitored within 20 days in early lactation. Diet of each groups had a balanced content of energy and crude protein, and a slight difference in lipid content. Goats in control group (n = 9) received soybean meal and extruded soybean. In experimental groups soybean was partially replaced with ELS (n = 10) or completely with PSC (n = 9). Dietary treatments had no effect on haemato-chemical parameters, although PSC increased concentration of globulins in serum. PSC lowered linoleic acid compared to control. ELS increased proportion of α-linolenic acid, eicosapentaenoic acid, and total n-3 fatty acids in milk. Positive correlations were found between non-esterified fatty acids (NEFA) and ratio C18:1/C15:0, NEFA and ratio C17:0/C15:0, as well as β-hydroxybutyrate and ratio C17:0/C15:0. The replacement of soybean meal with PSC or partially with ELS is feasible with respect to haemato-chemical parameters of early lactating dairy goats, while ELS had much beneficial effect on fatty acids compared to PSC.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":"71 1","pages":"13-24"},"PeriodicalIF":1.2,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41620794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.15567/mljekarstvo.2021.0105
Ç. Koçak
Kefiran is an exopolysaccharide (EPS) produced by specific microorganisms in the kefir grain. In this research, kefir grain was activated in various carbon sources (glucose, galactose or lactose) containing medium. The monosaccharides composition of the formed kefiran were determined by a high performance liquid chromatography (HPLC). HPLC with refractive index (RI) detector was used in order to make qualitative and quantitative analysis of sucrose, glucose, galactose, arabinose, xylose, and ribose present in kefiran. Also, the physical properties of differently kefiran formed were determined. The amount of kefiran in the control group and in the sample from the galactose containing medium were determined to be 511.00 and 610.55 mg/L, respectively. The kefiran form obtained from the medium containing galactose was more positively affected than in other media. The apparent viscosity, at a shear rate of 66.0 s-1of the kefiran produced in glucose containing medium, was determined to be 29.2 mPas. The surface morphologies of different kefirans were analysed and roughness parameters were determined by atomic force microscopy (AFM); the maximum height of lumps ranged from 16.80 to 22.10 nm. In this study, of sucrose, glucose, galactose, arabinose, xylose, and ribose were found in sugar composition in all kefiran samples.
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Pub Date : 2020-12-14DOI: 10.15567/MLJEKARSTVO.2021.0106
Gizem Şendoğan, Ecem Akan, O. Yerlikaya, Şaban Meriç, Ö. Kinik
In this study, the content of hippuric and benzoic acid of goat milk kefir produced with commercial lyophilized kefir cultures and kefir grain was investigated. 9 commercial kefir culture and 1 kefir grain were used in production of goat milk kefir. Based on the obtained results, hippuric acid was not found in kefir samples during the storage period, whereas goat milk samples contained hippuric acid (14.8 mg/kg). Benzoic acid levels of kefir samples ranged from 8.5 to 26.6 mg/kg during the 28-day storage period. The highest Lactobacillus and Lactococcus counts were observed in kefir sample produced with kefir grain while this sample was one of the sample containing the lowest (11.2 mg/kg) benzoic acid levels. In conclusion, the research results showed that starter kefir cultures and microbiota used in kefir production and storage period had a significant effect on the formation of benzoic acid levels (p<0.05).
{"title":"Changes in benzoic acid content of goat milk kefir produced using different kefir cultures","authors":"Gizem Şendoğan, Ecem Akan, O. Yerlikaya, Şaban Meriç, Ö. Kinik","doi":"10.15567/MLJEKARSTVO.2021.0106","DOIUrl":"https://doi.org/10.15567/MLJEKARSTVO.2021.0106","url":null,"abstract":"In this study, the content of hippuric and benzoic acid of goat milk kefir produced with commercial lyophilized kefir cultures and kefir grain was investigated. 9 commercial kefir culture and 1 kefir grain were used in production of goat milk kefir. Based on the obtained results, hippuric acid was not found in kefir samples during the storage period, whereas goat milk samples contained hippuric acid (14.8 mg/kg). Benzoic acid levels of kefir samples ranged from 8.5 to 26.6 mg/kg during the 28-day storage period. The highest Lactobacillus and Lactococcus counts were observed in kefir sample produced with kefir grain while this sample was one of the sample containing the lowest (11.2 mg/kg) benzoic acid levels. In conclusion, the research results showed that starter kefir cultures and microbiota used in kefir production and storage period had a significant effect on the formation of benzoic acid levels (p<0.05).","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":"71 1","pages":"60-68"},"PeriodicalIF":1.2,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41336653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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