Medicni perspektivi (Medical perspectives)最新文献

{"title":"Comparative analysis of aldehyde dehydrogenase 1 expression in polyps and adenocarcinoma of the distal colon","authors":"M. Shyshkin, T. O. Khrystenko","doi":"10.26641/2307-0404.2020.4.221242","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.4.221242","url":null,"abstract":"Comparative analysis of aldehyde dehydrogenase 1 expression in polyps and adenocarcinoma of the distal colon. Shyshkin M.A., Khrystenko T.O. Aldehyde dehydrogenase 1 (ALDH1) is one of the most widely used cancer stem cells markers, even though the question of its significance for certain stages of colorectal carcinogenesis is still unclear. The aim of this study was to compare ALDH1 immunohistochemical expression levels in polyps and adenocarcinoma of the distal colon. Histopathological and immunohistochemical studies of biopsies from 40 patients (biopsies of polyps and histologically non-changed mucosa of the distal colon), as well as surgical material of colorectal adenocarcinoma from 30 patients were carried out. It was established that distal colonic polyps are characterized by cytoplasmic expression of ALDH1 with the median of relative area that is equal to 25.63 (18.26; 30.42)% stromal cells. ALDH1 expression by epitheliocytes is revealed exclusively in dysplastic polyps with the median of the area that is equal to 22.13 (17.22; 30.05)%. Colorectal adenocarcinoma is characterized by cytoplasmic expression of ALDH1 with the medians of the area that are equal to 40,22 (22,54; 47,77)% stromal cells and 32,12 (23.64; 40.28)% cancer cells. The area of ALDH1+ cells varies depending on the pTNM stage of the carcinoma, herewith the median of ALDH1+ stromal cells is significantly increasing during the tumor progression from the I to the III stages that displays increasing in number of stromal cells which are involved in oncogenic signaling pathways activation. The median of ALDH1+ cancer cells is significantly increasing during the tumor progression from the III to the IV stages that shows increasing number of cancer cells that acquire properties of stem cells on advanced stages of colorectal adenocarcinoma. Comparative analysis of the parameters obtained for polyps and carcinoma indicates that the median of ALDH1+ stromal cells area in polyps is 2 times smaller than the median of ALDH1+ stromal cells area in carcinoma, and the median of ALDH1+ epitheliocytes area in polyps is 1.5 times smaller than the median of ALDH1+ cancer cells area. Реферат. Порівняльний аналіз експресії альдегіддегідрогенази 1 в поліпах і аденокарциномі дистальної товстої кишки. Шишкін М.А., Христенко Т.О. Альдегіддегідрогеназа 1 (ALDH1) є одним з найчастіше використовуваних маркерів ракових стовбурових клітин, проте питання щодо його значущості відносно окремих етапів колоректального канцерогенезу лишається до кінця не вивченим. Метою цього дослідження стало порівняння рівнів імуногістохімічної експресії ALDH1 в поліпах і аденокарциномі дистальної товстої кишки. Проведено патоморфологічне та імуногістохімічне дослідження біопсійного матеріалу 40 пацієнтів (біоптати поліпів і гістологічно незміненої слизової оболонки дистальної товстої кишки), а також операційного матеріалу колоректальної аденокарциноми 30 пацієнтів. Установлено, що поліпи дистальної товстої кишки характеризу","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75648535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective properties of n-phenylacetyl-l-prolylglycine ethyl ester nasal gel in an experimental model of multiple sclerosis equivalent","authors":"B. Burlaka, I. Belenichev, O. Nefedov, O. Aliyeva, N. Bukhtiyarova","doi":"10.26641/2307-0404.2020.4.221226","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.4.221226","url":null,"abstract":"The purpose of this research is to study the specific activity of our developed nasal dosage form with n-phenylacetyl-l-prolylglycine ethyl ester. The experiments were performed on 260 white outbred rats weighing 190-220 g. Experimental allergic encephalomyelitis (EAE) was induced by a single subcutaneous inoculation of an encephalitogenic mixture (EHM) in Complete Freund's Adjuvant (CFA) based on 100 mg of homologous spinal cord homogenate; 0.2 ml of CFA (the content of killed mycobacteria 5 mg/ml) and 0.2 ml of physiological saline per animal. There were five groups of animals in the study: 1) intact; 2) control - untreated with EAE, received saline solution; 3) animals with EAE that received basic treatment - methylprednisolone (MP), 3.4 mg/kg, intraperitoneally slowly in saline no more than 1/10 of the CBV rat; 4) animals with EAE treated with MP + Noopept, at a dose of 10 mg/kg; 5) animals with EAE treated with MP + Citicoline (Ceraxon, Ferrer Internacional S.A., Spain) D003U1 series, 500 mg/kg, intragastrically. The in­tegrative functions of rats’ brain with EAE were studied using the “Open Field” method with an arena of own production with dimensions 80x80x35 cm. The study of memory was carried out using the radial labyrinth LE760 (AgnTho's, Sweden). Capture and image recording was carried out using a color video camera SSC-DC378P (Sony, Japan). Video file analysis was performed using Smartv 3.0 software (Harvard Apparatus, USA). As a result of the stu­dies on experimental model of multiple sclerosis with a nasal gel containing ethyl ester of n-phenylacetyl-l-pro­lylglycine revealed the presence of normotimic activity, antidepressant and anxiolytic effects, an increase in the total activity of the central nervous system. The results obtained indicate a high neuroprotective and nootropic activity of the Noopept intranasal gel. By the degree of influence on reducing the number of errors in working memory, the Noopept gel sig­nificantly exceeds monotherapy with methylprednisolone and combination therapy with methylprednisolone and citi­coline. A further study of the effect of the developed nasal gel on the morphofunctional indices of sensorimotor cortical neurons under experimental multiple sclerosis, as well as on the content of HSP70 in the animal brain, is promising.","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79577490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimates of potentially preventable hospitalizations for ambulatory care sensitive conditions in Ukraine","authors":"V. Lekhan, L. Kriachkova, O. Doroshenko, L. O. Gritsenko","doi":"10.26641/2307-0404.2020.4.221711","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.4.221711","url":null,"abstract":"The purpose: this study aims to identify the most relevant diseases that can be treated outpatient (Ambulatory Care Sensitive Conditions, or ACSCs) and to define the scope of potentially preventable hospitalizations (PPHs) for such conditions (Ambulatory Care Sensitive Hospitalizations, or ACSH), for Ukrainian adults (above 18 years old), subject to improvement of primary care efficiency. The study has been carried out in four phases, using the World Health Organization’s (WHO) guidance on ACSCs for the European Region, which was adapted to the national context. The data was taken from the official statistical reporting and analyzed by 104 reviewers. The assessment of the PPHs is conducted based on responses of the subgroup of reviewers – 6 experts and 61 primary care physicians, which confirmed the levels of competencies. The most significant rates of potentially preventable hospitalizations (PPHs) are registered for hypertension, pneumonia, angina, chronic obstructive pulmonary disease (COPD), and diabetes, which coincide with ACSCs that are most prevalent for Ukrainian adults. The overall rate of PPHs for adult population of Ukraine with ACSCs is 28.2% as defined by physicians and 32.9% as defined by experts, which may reduce the hospitalization rate for all ACSCs (as percent of all patients with ACSCs) from 8.2% to 6.1% (according to reviews of physicians) or to 5.7% (according to experts’ reviews). The study has shown an important scope for better managing of ACSCs in Ukrainian adults. The results of the study can be used to inform the continued health system transformation, specifically for the development of interventions to prevent avoidable hospitalizations for ACSCs in Ukraine, which can lead to more efficient use of resources in the health system and further strengthening of the primary care.","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"10 9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83854479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Results of stress-strain states study in prosthetics of different types of atrophy of edentulous mandible","authors":"O. Fastovets, S. O. Sapalov, V. O. Shtepa","doi":"10.26641/2307-0404.2020.4.221411","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.4.221411","url":null,"abstract":"Complete dentures remain still popular due to the economic component of such prosthetic treatment. However, additional fixation on intraosseous implants provides a greater clinical and functional effectiveness and a greater level of satisfaction with the results of prosthetics in edentulous patients. At the moment, the biomechanical aspects of complete denture prosthetics and prosthetics with fixation on implants, taking into account the degree of atrophy of the edentulous jaws, are not complete understood. Such studies make it possible to create recommendations on the choice of prosthetic tactics for edentulous patients. The aim of the research was to study the distribution of stress-strain states in prosthetics of the edentulous mandible with complete dentures and with designs supported on intraosseous implants, taking into account the type of atrophy. It was carried out computer simulation of 8 virtual finite element models of mandible with different types of atrophy. According to Keller they were 4 models: of the biomechanical system simulation \"complete denture – mandible\" and 4 ones – \"denture – intraosseous implants – mandible\". In each of the models, a chewing load of 100 N was simulated (symmetrically and asymmetrically). The ANSIS 12.1 finite element analysis was used to calculate the stress-strain states in the described calculation systems. It was estimated the distribution of stresses in cortical bone and displacements of the dentures on the prosthetic bed. Under using complete dentures, the maximum stresses in the bone tissue of the prosthetic bed were observed for the third type of mandible atrophy with all types of power load, the smallest ones – for the fourth type. Additional fixation of removable dentures in simulation models of biomechanical systems “denture – intraosseous implants – mandible” lead to a significant increase in stresses in the alveolar bone. The maximum stresses were observed in the area of the marginal bone, while their greatest values were in the well-expressed alveolar part of the mandible for first and third types by Keller. The movements of bases of complete dentures were insignificant and fluctuated within hundredths of a millimeter for all types of atrophy. The use of intraosseous implants for fixation of dentures caused increase in movements by several times. Besides, displacement fields were characteristic: they were uniform for complete dentures but in the use of implants – not. The expressed alveolar process in the first and third types of atrophy of the edentulous mandible caused an increase in the displacements of the distal sections of the dentures on both sides with a symmetrical force load and on one side – with an asymmetric one. It can be assumed that such a distribution of stress-strain states accelerates atrophy of prosthetic bed tissues. As a result of prosthetics of complete defects of lower dentitions, both with traditional complete dentures and with additional support on implants, differ","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88329990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and laboratory predictors of antitoxic immunity against diphtheria and tetanus in adults with HIV infection","authors":"H. Revenko, V. Mavrutenkov, Z. Chykarenko","doi":"10.26641/2307-0404.2020.3.214846","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214846","url":null,"abstract":"Clinical and laboratory predictors of antitoxic immunity against diphtheria and tetanus in adults with HIV infection. Revenko H.O., Mavrutenkov V.V., Chykarenko Z.O. Antiretroviral therapy has made HIV infection a chronic controlled disease, where aspects of the immunoprophylaxis of infectious diseases have acquired important clinical significance. The goal of the study was to determine the clinical and laboratory predictors of antitoxic immunity against diphtheria and tetanus in HIV-infected adults. The study included 90 HIV-infected patients aged 22 to 60 years (main group). The control group consisted of 49 immunocompetent volunteers of the corresponding age. The levels of anti-diphtheria and anti-tetanus antibodies were determined by ELISA using the diagnostic test systems RIDASCREEN Diphtheria IgG and RIDASCREEN Tetanus IgG (R-Biopharm AG, Germany). Statistical processing was performed using the licensed software product STATISTICA v.6.1. Significant differences were found between the titers of antitoxic antibodies in HIV-infected and immunocompetent adults. According to the correlation analysis, the decrease in the titers of antidiphtheria antibodies was revealed with an increase in the age of the HIV-infected patient (rs=-0.21; p=0.05). The fact of smoking (rs=-0.31; p=0.003), lowered body weight (BMI<18.5 kg/m) (rs=-0.29; p=0.006), the presence of arterial hypertension (rs=-0.38; p<0.001), a history of bone fractures over the past 5 years (rs=-0.38; p<0.001), anemia (rs=-0.21; p=0.049), thrombocytopenia (rs=-0.44; p<0.001), accelerated ESR (rs=-0.61; p<0.001), the presence of hairy leukoplakia of the tongue (rs=-0.23; p=0.027), frequent infections caused by herpes simplex (rs=0.52; p=0.003) and varicella zoster virus (rs=-0.34; p=0.013) are associated with low levels of antidiphtheria antibodies. A direct relationship was found between the intensity of anti-diphtheria immunity and patients receiving OST (rs=+0.54; p=0.003) and with a history of injuries with impaired skin integrity (rs=+0.31; p=0.003). Decreased anti-tetanus immunity in HIV-infected patients was also associated with smoking (rs=-0.48; p<0.001), decreased BMI (rs=-0.71; p<0.001), anemia (rs=-0.33; p=0.002), thrombocytopenia (rs=-0.75; p<0.001), a history of bone fractures over the past 5 years (rs=-0.67; p<0.001); the total number of HIV-associated opportunistic diseases (rs=-0.42; p<0.001), including the presence of oropharyngeal candidiasis (rs=-0.23; p=0.032) and hairy leukoplakia of the tongue (rs=-0.57; p<0.001), history of Herpes Zoster in the past (rs=-0.48; p<0.001), with frequent relapses of diseases caused by herpes simplex viruses (rs=-0.78; p<0.001) and repeated episodes of herpes zoster (rs=-0.74; p<0.001), as well as with pathology of the skin (rs=-0.55; p<0.001). Protective predictors of anti-tetanus antitoxic immunity strength were established: male gender (rs=+0.22; p=0.039), parenterally acquired HIV infection (rs=+0.21; p=0.05), HIV-infected patients rec","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"110 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73570629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles in the air of the working zone as a risk factor for the health of workers of various industries","authors":"A. Sevalnev, L. P. Sharavara, A. Kutsak, O. Nefodov, O. A. Zemliynyi, K. I. Pisarevskyi, O. S. Shevchenko","doi":"10.26641/2307-0404.2020.3.214859","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214859","url":null,"abstract":"Purpose: analysis of scientific literature, summarizing data on domestic and foreign experience of assessing the determination of nanoparticles in the air of the working zone as a risk factor for the health of workers of various industries. The article analyzes the literature data on the study of the content of fine dust and nanoparticles in the atmospheric air and air of the working zone of different industries. Numerous studies indicate that fine dust is contained in the emissions of many industrial enterprises. According to the World Health Organization by level of impact on human health, suspended particles in the air and especially in the air of the working zone belong to the priority pollutants. Evaluation of the dust content in the air of large industrial cities is particularly relevant, because of a large number of sources of dust emissions of various origins in urban areas. Various technological processes contribute to the formation of fine dust and nanoparticles which pollute the ambient air and the air of the working zone. Data on the negative impact of fine dust and nanoparticles on health of workers are presented. Attention is paid to the problem of hygienic assessment of nanoscale dust content in the working zone air. The obtained results indicate that today the issues of studying the physicochemical properties of nanoparticles, their toxicity to the body, analysis of potential risks to humans, the development of an effective and reliable system for monitoring ultrafine particles in the environment and the production environment are still relevant as for informing employees about the risks involved, reducing and preventing harmful effects on humans. The potential negative effects on workers’ health determine the need and opportunity for further research in this area.","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87268286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"West Nile Fever in the central part of Ukraine","authors":"T. M. Kotelevska, N. O. Pryimenko, H. M. Dubynska, T. Koval, O. M. Iziumska, I. M. Zviagolska","doi":"10.26641/2307-0404.2020.3.214874","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214874","url":null,"abstract":"204 На умовах ліцензії CC BY 4.0 (nondirected altruistic) kidney donors in the United Kingdom. Transplantation. 2014;98:1182. doi: https://doi.org/10.1097/TP.0000000000000340 20. Northam H. Increasing consent to organ donation by enabling donor families and recipients to meet. Donor Families Australia News. 2017;18:10. Available from: https://docs.wixstatic.com/ugd/8a154b_c40154f232ad4e1 bb43c525f3c4de9ae.pdf 21. Annema C, et al. Opinions of Dutch Liver Transplant Recipients on Anonymity of Organ Donation and Direct Contact With the Donors Family. Transplantation. 2014;99(4). doi: https://doi.org/10.1097/TP.0000000000000394 22. Poole J. Donor Family and Recipient Anonymity: Time for Change. The Journal of Heart and Lung Transplantation. 2019;38(4):S92-3. doi: https://doi.org/10.1016/j.healun.2019.01.214 23. Post M. What matters and what doesn’t: Organ, eye and tissue donor families, grief support and recipient communication. Grief Matters: The Australian Journal of Grief and Bereavement. 2015;18(1):18-22. Available from: https://search.informit.com.au/documentSummary;dn=3","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78382805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low rates of restenosis in primary lateral carotid artery endarterectomy","authors":"A. Mackevičius, V. Mosenko, K. Laurikėnas, A. Sataviciute, T. Baltrūnas, U. Chernyaha-Royko, N. Demkova, L. Bardachenko","doi":"10.26641/2307-0404.2020.3.214818","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214818","url":null,"abstract":"Low rates of restenosis in primary lateral carotid artery endarterectomy. Mackevičius A., Mosenko V., Laurikėnas K., Šatavičiūtė A., Baltrūnas T., Chernyaha-Royko U., Demkova N., Bardachenko L. Carotid artery endarterectomy (CAE) is a treatment of choice for symptomatic and asymptomatic high-grade carotid stenosis, showing great results in reducing stroke morbidity. The optimal technique of the arterial closure is, however, still under discussion, with both patch angioplasty and primary closure having numerous advantages and pitfalls. The definite evidence is still lacking. The aim of this study was to evaluate the results of the modified primary closure technique during CEA. Incidence of restenosis more than 8 months after the surgery was measured. A retrospective observational","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83832734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When the limited pelvic lymph node dissection in prostate cancer patients can be justified?","authors":"R. Molchanov, E. Stakhovskyi, L. Kriachkova, Ye. V. Pilin, S. L. Malinovskyi","doi":"10.26641/2307-0404.2020.3.214848","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214848","url":null,"abstract":"When the limited pelvic lymph node dissection in prostate cancer patients can be justified? Molchanov R.M., Stakhovskyi E.O., Kriachkova L.V., Pilin Ye.V., Malinovskyi S.L. Extended pelvic lymph node dissection (PLND) is an important diagnostic step in the surgical treatment of moderate and high risk prostate cancer (PCa) according to D'Amico criteria. However, it has a number of complications and prolonged time of surgery. Limited PLND has a more favorable complication profile, but is not used because of its low diagnostic efficacy in lowrisk RP patients, while in higher-risk groups its relevance remains controversial. The goal – to determine the diagnostic efficacy of limited PLND in radical prostatectomy in patients of moderate and high risk. A retrospective analysis included 377 PCa patients in whom the radical prostatectomy with PLND was performed in the period between 2013 and 2016. Patients' age was 63.4±6.2 y.o. 40 (10.6%) patients had low, 126 (33.4%) – moderate and 211 (56.0%) – high risk PCa. No statistically significant differences in the number of complications of PLND in open and laparoscopic МЕДИЧНІ ПЕРСПЕКТИВИ / MEDICNI PERSPEKTIVI 125 20/ Том XXV / 3 surgery (p=0.16) were found. The overall frequency of complications was 22.8% (95% CI 18.6 27.1). When comparing clinical and histological parameters in groups with and without metastases, statistically significant differences were found between the levels of total prostate specific antigen before surgery (p=0.010); the Gleason score (corresponding median values of 8.0 (8.0; 9.0) and 7.0 (6.0; 7.0); p<0.001) and local tumor status (T) – the patients with stage >T2 53.1% and 19.4%, respectively (p<0.001). In 32 (8.5%) patients metastatic lesions of lymphatic nodes were found. Of these, 28 (87.5%) were related to highrisk, 4 (12.5%) – to moderaterisk. The main prognostic criteria for lymph node metastasis are preoperative PSA level, the Gleason Score, and T-status of the tumor. According to ROC analysis, the diagnostic efficacy of limited PLND increases in patients at high and moderate risk at a total PSA level greater than 18.4 ng/ml. This can be used to justify the indications for limited PLND in patients in these groups to reduce the number of postoperative complications associated with extended procedure. Реферат. Коли обмежена тазова лімфаденектомія у хворих на рак передміхурової залози може бути виправданою? Молчанов Р.М., Стаховський Е.О., Крячкова Л.В., Пілін Є.В., Маліновський С.Л. Розширена тазова лімфаденектомія (ТЛАЕ) є важливим лікувально-діагностичним етапом при хірургічному лікуванні раку передміхурової залози (РПЗ) помірного і високого ризику за критеріями D’Amico. Проте вона має низку ускладнень і подовження часу оперативного втручання. Обмежена ТЛАЕ має більш сприятливий профіль ускладнень, проте не використовується внаслідок її низької діагностичної ефективності у хворих на РПЗ низького ризику, у той час як у групах більшого ризику її доцільність залишається дискутаб","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83919436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of chronic generalized periodontitis in laboratory animals (literature review)","authors":"O. V. Kopchak, N. Marchenko, Ya. V. Yanishevska","doi":"10.26641/2307-0404.2020.3.214646","DOIUrl":"https://doi.org/10.26641/2307-0404.2020.3.214646","url":null,"abstract":"Modeling of chronic generalized periodontitis in laboratory animals (literature review). Kopchak O.V., Marchenko N.S., Yanishevska Ya.V. Modern experiments in the study of periodontal deseas are aimed at improving the effectiveness of innovative methods of early diagnosis, treatment and prevention of this disease in patients. The conducted analysis of the literature showed that the disorder of microcirculation occupies one of the leading places in the pathogenesis of periodontitis. Disruption of the microcirculatory bed, based on the dyscirculatory changes of the capillary blood flow is accompanied by a decrease in the intensity of blood filling of vessels, their spasm. These changes, in case of chronization, include tissue disorders of the ischemic nature, up to the development of a capillary stasis. This can lead to disorder in gas exchange, trophism and pathomorphological damage to periodontal tissues. This type of change is most typical for agerelated, traumatic, vascular, and stress-induced models. Morphological lesions in the vessels, namely ischemic phenomena were most pronounced in them. Microscopically, this was manifested in the heterogeneity of the epithelial layer, the thickening of individual layers, the detachment of the epithelial lining of the mucosa. As for the basal layer, the most common was the vacuolization of cells, the appearance of a significant number of cells that lacked nuclei (pathology, indicating a decrease in functional activity, impaired regenerative properties of the cell), expansion of intercellular contacts, a sign of tissue edema. In the basal membrane (function dissociation of the epithelium from the actual lining of the mucous membrane), collagen fibers were loose, hypertrophic, their hyperplasia was observed, neutrophil leukocytes, lymphocytes, histocytes were found between the fibers, this testified to changes in cells. Some models lack a clinical picture of chronic hyperplastic periodontitis. The disorders consisted of changes in normal metabolism in periodontal tissues, decreased immune function, changes in morphological structure without induction of the inflammatory process. Реферат. Моделювання хронічного генералізованого пародонтиту в лабораторних тварин (огляд літератури). Копчак О.В., Марченко Н.С., Янішевська Я.В. Сучасні експерименти в дослідженні захворювань пародонту спрямовані на підвищення ефективності інноваційних методів ранньої діагностики, лікування та профілактики цього захворювання в пацієнтів. Проведений аналіз літератури показав, що розлад мікроциркуляції посідає одне з провідних місць у патогенезі пародонтиту. Порушення мікроциркуляторного русла, що базується на дисциркуляторних змінах капілярного кровотоку, супроводжується зниженням інтенсивності кровонаповнення судин, їх спазмом. Ці зміни у випадку хронізації призводять до тканинних порушень ішемічного характеру, аж до розвитку капіляного стазу. Це може призвести до порушення газообміну, трофіки та патоморфологічного ураження тканин","PeriodicalId":18652,"journal":{"name":"Medicni perspektivi (Medical perspectives)","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86099514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}