Methods and Protocols最新文献_第2页

Odours in Asphalt: Analysis of the Release of H2S from Bitumen by a Mass Spectrometric Residual Gas Analyser 沥青中的气味：用质谱残余气体分析仪分析沥青中释放的 H2S

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-07-15 DOI: 10.3390/mps7040055

Marcello Monteleone, Giuseppe Di Luca, Marcello Filomia, A. Fuoco, A. Figoli, J. C. Jansen

During the production and laying phases of hot-mixing asphalt (HMA), various volatile organic compounds (VOCs) and noxious gases such as H2S are released into the atmosphere. These emissions are a serious environmental problem, a risk to human health, and expose workers and residents to unfriendly odours. The aim of this study was the development of a fast and sensitive analytical method to detect the H2S emitted from hot bituminous binder that is generally used in the various stages of asphalt production, processing, handling and during road construction. The method consisted in the analysis of evolved H2S from a flask with molten bitumen, using nitrogen as a carrier gas to lead the volatile compounds into a residual gas analyser equipped with a quadrupole mass spectrometer. The analysis was performed following the H2S-specific signals at m/z 33 (HS+) and at m/z 34 (H2S+) in real time, directly on the sample without laborious and expensive pre-treatments and with short response times (<6 s). Calibration with a standard mixture of 1000 ppm of H2S in nitrogen allows semi-quantitative H2S detection. The sensitivity and rapidity of the method were evaluated by quenching the release of sulphur compounds with commercial odour-suppressing agents. Upon addition of 0.1% of additive in two minutes, the H2S signal drops about 80% in two minutes, confirming the good response of the method, even with a very complex matrix.

在热拌沥青（HMA）的生产和铺设阶段，各种挥发性有机化合物（VOC）和有害气体（如 H2S）会释放到大气中。这些排放物是一个严重的环境问题，对人类健康构成威胁，并使工人和居民受到不友好气味的影响。这项研究的目的是开发一种快速灵敏的分析方法，用于检测热沥青粘结剂排放的 H2S，这种粘结剂通常用于沥青生产、加工、处理和道路建设的各个阶段。该方法包括分析从装有熔融沥青的烧瓶中挥发出来的 H2S，使用氮气作为载气，将挥发性化合物导入配备四极质谱仪的残余气体分析仪。分析是根据 m/z 33（HS+）和 m/z 34（H2S+）处的 H2S 特定信号实时进行的，直接在样品上进行，无需费力和昂贵的预处理，响应时间短（<6 秒）。使用氮气中 1000 ppm 的 H2S 标准混合物进行校准，可实现半定量 H2S 检测。通过使用商用气味抑制剂淬灭硫化合物的释放，对该方法的灵敏度和快速性进行了评估。在两分钟内加入 0.1% 的添加剂后，H2S 信号在两分钟内下降了约 80%，这证实了该方法即使在基质非常复杂的情况下也能做出良好的反应。

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引用次数: 0

Protocol for the Development and Initial Validation of the COG-IMPACT Tool: A Purpose-Built Unmet Needs Assessment for Cancer-Related Cognitive Impairment COG-IMPACT 工具的开发和初步验证协议：针对癌症相关认知障碍的未满足需求评估工具

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-07-10 DOI: 10.3390/mps7040054

Darren Haywood, Moira O’Connor, Frank D. Baughman, Alexandre Chan, Raymond J. Chan, Evan Dauer, Haryana M. Dhillon, Ashley M. Henneghan, Blake J. Lawrence, Maryam Lustberg, Janette L Vardy, S. L. Rossell, Nicolas H. Hart

(1) Background: A significant proportion of cancer survivors report experiencing a cognitive ‘fog’ that affects their ability to think coherently and quickly, and reason with clarity. This has been referred to as cancer-related cognitive impairment (CRCI). CRCI has extensive impacts on the daily lives of people living with or beyond cancer, including occupational, social, and psychological functioning. Oncology health professionals report feeling under-resourced to effectively assess the needs of an individual with CRCI and then provide optimal care and referral. (2) Methods: The objective of this project is to develop and provide an initial validation of the first purpose-built unmet needs assessment for CRCI: the Unmet Needs Assessment of Cancer-Related Cognitive Impairment Impact (COG-IMPACT). We will use a multiple-stage, co-design, mixed-methods approach to develop and provide an initial validation of the COG-IMPACT. (3) Results: The primary anticipated result of this research is the production of the COG-IMPACT, the first purpose-built unmet needs assessment for CRCI. The assessment could be used by health professionals to understand the unmet needs and facilitate optimal care and referral for cancer survivors, by survivors to elucidate their supportive needs and advocate for their care, and by researchers to examine the correlates of unmet needs relating to CRCI, as well as how best to support people with CRCI.

(1) 背景：有相当一部分癌症幸存者表示，他们经历过认知 "迷雾"，这影响了他们连贯、快速思考和清晰推理的能力。这种情况被称为癌症相关认知障碍（CRCI）。CRCI 对癌症患者或癌症后患者的日常生活造成了广泛影响，包括职业、社交和心理功能。肿瘤科医护人员表示，在有效评估 CRCI 患者的需求并提供最佳护理和转诊方面，他们感到资源不足。(2) 方法：本项目的目标是开发首个专门针对 CRCI 的未满足需求评估工具：癌症相关认知功能障碍未满足需求评估工具 (COG-IMPACT)，并对其进行初步验证。我们将采用多阶段、共同设计、混合方法来开发 COG-IMPACT 并对其进行初步验证。(3) 成果：本研究的主要预期成果是编制 COG-IMPACT，这是首个专门针对 CRCI 的未满足需求评估。健康专业人员可利用该评估来了解未满足的需求，并促进为癌症幸存者提供最佳护理和转诊服务；幸存者可利用该评估来阐明其支持性需求，并倡导为其提供护理；研究人员可利用该评估来研究与 CRCI 相关的未满足需求的相关性，以及如何为 CRCI 患者提供最佳支持。

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引用次数: 0

Suitability of XRF for Routine Analysis of Multi-Elemental Composition: A Multi-Standard Verification XRF 对多元素成分常规分析的适用性：多标准验证

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-07-05 DOI: 10.3390/mps7040053

Riccardo Fedeli, L. D. Di Lella, Stefano Loppi

This study investigated the suitability of X-ray fluorescence (XRF) analysis for routine multi-elemental composition analysis, checking its analytical capabilities by measuring a wide array of certified reference materials of soil and plant origin. A portable XRF analyzer was used to evaluate 32 soil and 12 plant standard materials, using both the Soil and Geochem mode, with sequential beams, allowing the detection of a wide range of elements. Recovery rates were calculated by comparing XRF measurements with certified values, and their correlations were verified through the Spearman coefficient. The results demonstrated the reliability of XRF measurements for soil samples, with a large number of elements showing a good or very good recovery and strong correlations with certified values. For plant samples, XRF largely overestimated the certified values, but the strong statistically significant correlations for almost all tested elements allowed us to correct this systematic bias, using the reported median value for dividing the value obtained via XRF. The Geochem mode emerged as more reliable for a larger number of elements. It was concluded that XRF may be a suitable alternative to ICP-MS in routine multi-elemental composition analysis.

本研究调查了 X 射线荧光（XRF）分析法在常规多元素成分分析中的适用性，并通过测量一系列经认证的土壤和植物参考材料来检验其分析能力。使用便携式 X 射线荧光分析仪评估了 32 种土壤和 12 种植物标准材料，采用土壤和地球化学模式，连续光束可检测多种元素。通过比较 XRF 测量值和认证值，计算出回收率，并通过斯皮尔曼系数验证其相关性。结果表明 XRF 测量对土壤样本的可靠性，大量元素的回收率良好或非常好，并与认证值有很强的相关性。对于植物样本，XRF 很大程度上高估了认证值，但几乎所有测试元素都有很强的统计意义上的相关性，这使我们能够纠正这种系统性偏差，使用报告的中值来除以通过 XRF 获得的值。对于更多元素而言，Geochem 模式更为可靠。结论是，在常规多元素成分分析中，XRF 可能是 ICP-MS 的合适替代品。

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引用次数: 0

Methods to Investigate the Secretome of Senescent Cells 研究衰老细胞分泌组的方法

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-07-02 DOI: 10.3390/mps7040052

Afshin Samiminemati, Domenico Aprile, Dario Siniscalco, G. Di Bernardo

The word “secretome” was first used to describe the proteins that cells secrete under different circumstances; however, recent studies have proven the existence of other molecules such as RNA and chemical compounds in the secretome. The study of secretome has significance for the diagnosis and treatment of disease as it provides insight into cellular functions, including immune responses, development, and homeostasis. By halting cell division, cellular senescence plays a role in both cancer defense and aging by secreting substances known as senescence-associated secretory phenotypes (SASP). A variety of techniques could be used to analyze the secretome: protein-based approaches like mass spectrometry and protein microarrays, nucleic acid-based methods like RNA sequencing, microarrays, and in silico prediction. Each method offers unique advantages and limitations in characterizing secreted molecules. Top-down and bottom-up strategies for thorough secretome analysis are became possible by mass spectrometry. Understanding cellular function, disease causes, and proper treatment targets is aided by these methodologies. Their approaches, benefits, and drawbacks will all be discussed in this review.

分泌组 "一词最初用于描述细胞在不同情况下分泌的蛋白质，但最近的研究证明，分泌组中还存在其他分子，如核糖核酸和化合物。对分泌物组的研究对疾病的诊断和治疗具有重要意义，因为它能让人们深入了解细胞的功能，包括免疫反应、发育和稳态。细胞衰老通过停止细胞分裂，分泌称为衰老相关分泌表型（SASP）的物质，在癌症防御和衰老中发挥作用。有多种技术可用于分析分泌组：基于蛋白质的方法，如质谱法和蛋白质微阵列；基于核酸的方法，如 RNA 测序、微阵列和硅预测。每种方法在描述分泌分子特征方面都有其独特的优势和局限性。质谱技术使自上而下和自下而上彻底分析分泌组的策略成为可能。这些方法有助于了解细胞功能、疾病原因和适当的治疗目标。本综述将讨论它们的方法、优点和缺点。

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引用次数: 0

A Review of Histological Techniques for Differentiating Human Bone from Animal Bone. 区分人类骨骼与动物骨骼的组织学技术综述。

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-06-30 DOI: 10.3390/mps7040051

Emanuela Stan, Camelia-Oana Muresan, Ecaterina Daescu, Raluca Dumache, Veronica Ciocan, Stefania Ungureanu, Dan Costachescu, Alexandra Enache

The first step in anthropological study is the positive identification of human remains, which can be a challenging undertaking when bones are broken. When bone pieces from different species are mixed together, it can be crucial to distinguish between them in forensic and archaeological contexts. For years, anthropology and archaeology have employed the histomorphological analysis of bones to evaluate species-specific variations. Based on variations in the dimensions and configuration of Haversian systems between the two groups, these techniques have been devised to distinguish between non-human and human bones. All of those techniques concentrate on a very particular kind of bone, zone, and segment. Histomorphometric techniques make the assumption that there are size, form, and quantity variations between non-humans and humans. The structural components of Haversian bones are significant enough to use discriminant function analysis to separate one from the other. This review proposes a comprehensive literature analysis of the various strategies or techniques available for distinguishing human from non-human bones to demonstrate that histomorphological analysis is the most effective method to be used in the case of inadequate or compromised samples.

人类学研究的第一步是对人类遗骸进行积极的鉴定，当骨头断裂时，这可能是一项具有挑战性的工作。当来自不同物种的骨片混杂在一起时，在法医和考古学背景下区分它们可能至关重要。多年来，人类学和考古学一直采用骨骼组织形态学分析来评估物种的具体差异。根据两类骨骼在哈弗斯系统的尺寸和构造上的差异，这些技术被用来区分非人类骨骼和人类骨骼。所有这些技术都集中于一种非常特殊的骨骼、区域和部分。组织形态计量技术假定非人类和人类在大小、形态和数量上存在差异。哈弗类骨骼的结构成分非常重要，足以使用判别函数分析法将它们区分开来。本综述对现有的区分人类和非人类骨骼的各种策略或技术进行了全面的文献分析，以证明在样本不足或受损的情况下，组织形态学分析是最有效的方法。

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引用次数: 0

In Vitro Methods for Assessing the Antibacterial and Antibiofilm Properties of Essential Oils as Potential Root Canal Irrigants-A Simplified Description of the Technical Steps. 评估作为潜在根管灌洗剂的精油的抗菌和抗生物膜特性的体外方法--技术步骤简述。

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols

Pub Date : 2024-06-25 DOI: 10.3390/mps7040050

Jihad Diouchi, Jelena Marinković, Milica Nemoda, Lhoussaine El Rhaffari, Babacar Toure, Sonia Ghoul

Background: Essential oils have gained in significance due to their various biological activities, and there is a growing demand for them in many industries. The present article focuses on the technical steps for an in vitro evaluation of the antibacterial and antibiofilm activities of essential oils for potential use as root canal irrigant in dentistry.

Methods: The bioactivities of the essential oil were investigated through in vitro assays. The gram-positive bacterium Enterococcus faecalis was used as a micro-organism model. The antibacterial activity of the essential oil was assessed using the microdilution method, and resazurin staining to determine the minimal inhibition concentrations (MICs) and the minimal bactericidal concentrations (MBCs). The antibiofilm effect was evaluated spectrophotometrically at 570 nm using the microplate cultivation technique and crystal violet staining.

Conclusions: This article features a detailed in vitro protocol to facilitate the preparation of the essential oil samples, the bacterial suspension, and the methods used for assessment of the antibiofilm and antibacterial activities of the essential oil. The advantages of these approaches are presented in relation to the limits linked to the choice of the bacteria and the essential oil.

背景：精油因其多种生物活性而变得越来越重要，许多行业对精油的需求也在不断增长。本文重点介绍了对精油的抗菌和抗生物膜活性进行体外评估的技术步骤，以便将其用作牙科根管冲洗剂：方法：通过体外试验研究了精油的生物活性。以革兰氏阳性菌粪肠球菌为微生物模型。精油的抗菌活性采用微量稀释法和树脂醛染色法进行评估，以确定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。采用微孔板培养技术和水晶紫染色法，在 570 纳米波长处用分光光度法评估了抗生物膜效果：本文提供了一个详细的体外实验方案，便于制备精油样品、细菌悬浮液以及用于评估精油抗生物膜和抗菌活性的方法。文章介绍了这些方法的优点，以及与细菌和精油的选择有关的限制。

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引用次数: 0

Simple Determination of Affinity Constants of Antibodies by Competitive Immunoassays 用竞争性免疫测定法简单测定抗体的亲和常数

IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology

Methods and Protocols

Pub Date : 2024-06-13 DOI: 10.3390/mps7030049

Janina Fischer, Jan Ole Kaufmann, Michael G. Weller

The affinity constant, also known as the equilibrium constant, binding constant, equilibrium association constant, or the reciprocal value, the equilibrium dissociation constant (Kd), can be considered as one of the most important characteristics for any antibody–antigen pair. Many methods based on different technologies have been proposed and used to determine this value. However, since a very large number of publications and commercial datasheets do not include this information, significant obstacles in performing such measurements seem to exist. In other cases where such data are reported, the results have often proved to be unreliable. This situation may indicate that most of the technologies available today require a high level of expertise and effort that does not seem to be available in many laboratories. In this paper, we present a simple approach based on standard immunoassay technology that is easy and quick to perform. It relies on the effect that the molar IC50 approaches the Kd value in the case of infinitely small concentrations of the reagent concentrations. A two-dimensional dilution of the reagents leads to an asymptotic convergence to Kd. The approach has some similarity to the well-known checkerboard titration used for the optimization of immunoassays. A well-known antibody against the FLAG peptide, clone M2, was used as a model system and the results were compared with other methods. This approach could be used in any case where a competitive assay is available or can be developed. The determination of an affinity constant should belong to the crucial parameters in any quality control of antibody-related products and assays and should be mandatory in papers using immunochemical protocols.

亲和力常数，又称平衡常数、结合常数、平衡关联常数，或其倒数值--平衡解离常数（Kd），可被视为任何抗体-抗原配对的最重要特征之一。许多基于不同技术的方法已被提出并用于确定该值。然而，由于大量的出版物和商业数据表都不包含这一信息，因此在进行此类测量时似乎存在很大的障碍。在报告了此类数据的其他情况下，结果往往被证明是不可靠的。这种情况可能表明，当今的大多数技术都需要高水平的专业知识和努力，而许多实验室似乎并不具备这种能力。在本文中，我们介绍了一种基于标准免疫测定技术的简单方法，该方法简便快捷。它依赖于在试剂浓度无限小的情况下摩尔 IC50 接近 Kd 值的效应。试剂的二维稀释会导致向 Kd 的渐近收敛。这种方法与著名的用于优化免疫测定的棋盘滴定法有些相似。我们使用了一种著名的 FLAG 肽抗体克隆 M2 作为模型系统，并将结果与其他方法进行了比较。这种方法适用于有竞争性检测方法或可以开发竞争性检测方法的任何情况。亲和力常数的测定应属于抗体相关产品和检测质量控制的关键参数，在使用免疫化学方案的论文中也应强制使用。

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引用次数: 0

A Comprehensive Methodology for Microbial Strain Typing Using Fourier-Transform Infrared Spectroscopy 利用傅立叶变换红外光谱进行微生物菌种分类的综合方法

IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology

Methods and Protocols

Pub Date : 2024-06-11 DOI: 10.3390/mps7030048

F. Muchaamba, Roger Stephan

Timely and accurate detection and characterization of microbial threats is crucial for effective infection and outbreak management. Additionally, in food production, rapid microbe identification is indispensable for maintaining quality control and hygiene standards. Current methods for typing microbial strains often rely on labor-intensive, time-consuming, and expensive DNA- and sera-serotyping techniques, limiting their applicability in rapid-response scenarios. In this context, the IR Biotyper®, utilizing Fourier-transform infrared (FTIR) spectroscopy, offers a novel approach, providing specific spectra for fast strain typing within 3 h. This methodology article serves as a comprehensive resource for researchers and technicians aiming to utilize FTIR spectroscopy for microbial strain typing. It encompasses detailed guidelines on sample preparation, data acquisition, and analysis techniques, ensuring the generation of reliable and reproducible results. We highlight the IR Biotyper®’s rapid and accurate discrimination capabilities, showcasing its potential for real-time pathogen monitoring and source-tracking to enhance public health and food safety. We propose its integration as an early screening method, followed by more detailed analysis with whole-genome sequencing, to optimize detection accuracy and response efficiency in microbial surveillance systems.

及时准确地检测微生物威胁并确定其特征对于有效管理感染和疫情爆发至关重要。此外，在食品生产中，快速鉴定微生物对于保持质量控制和卫生标准也是不可或缺的。目前对微生物菌株进行分型的方法通常依赖于劳动密集型、耗时且昂贵的 DNA 和血清分型技术，这限制了它们在快速反应场景中的适用性。在这种情况下，利用傅立叶变换红外（FTIR）光谱的 IR Biotyper® 提供了一种新方法，可在 3 小时内为快速菌株分型提供特异性光谱。它包含有关样品制备、数据采集和分析技术的详细指南，可确保生成可靠、可重复的结果。我们强调了 IR Biotyper® 快速准确的分辨能力，展示了其在病原体实时监测和来源追踪方面的潜力，以提高公共卫生和食品安全。我们建议将其整合为一种早期筛选方法，然后利用全基因组测序进行更详细的分析，以优化微生物监测系统的检测准确性和响应效率。

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引用次数: 0

Evaluation of the Sexually Transmitted Infections Programme among Key and Priority Populations in Primary Healthcare Facilities to Inform a Targeted Response: A Protocol Paper 评估基层医疗机构重点人群和优先人群中的性传播感染计划，为有针对性的应对措施提供依据：协议文件

IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology

Methods and Protocols

Pub Date : 2024-06-05 DOI: 10.3390/mps7030047

M. A. Seloka, Edith Phalane, R. Phaswana-Mafuya

Primary healthcare facilities lack routine diagnostic screening due to resource limitations and dependence on syndromic management, resulting in an unprecedented prevalence and incidence of sexually transmitted infections (STIs), particularly among key and priority populations. Specific focuses are essential to strengthen current STI control measures. Therefore, this article describes the protocol for evaluating STI programme among key and priority populations in selected primary healthcare facilities in South Africa. We will employ an exploratory, descriptive research design to assess the STI programme in terms of its facility operations, functions, scope, gaps, delivery services, STI surveillance methods, and indicators in the selected primary healthcare facilities. A purposive sample of 15–20 STI programme stakeholders will be selected from five primary healthcare facilities in Limpopo Province, South Africa. The programme evaluation will use the World Health Organization assessment checklist tool, a globally recognised and validated instrument comprising open- and closed-ended questions to assess the STI programme. This tool, known for its credibility and reliability, ensures the study’s validity. Quantitative data will be captured on STATA software (College Station, TX, USA) version 18 for descriptive analysis and presented as the mean and standard deviation for continuous variables, proportions and percentages for categorical variables. A p ≤ 0.05 will demonstrate a statistically significant level. Thematic content analysis will be conducted for the qualitative data using Atlas. ti software (Technical University, Berlin, Germany) version 23.1. The study’s results will inform new approaches to strengthen STI coverage, service delivery, and linkage to care.

由于资源限制和对综合症管理的依赖，基层医疗机构缺乏常规诊断筛查，导致性传播感染（STI）的流行率和发病率达到前所未有的水平，尤其是在重点人群和优先人群中。要加强当前的性传播感染控制措施，就必须突出重点。因此，本文介绍了在南非选定的初级医疗保健机构中对重点人群和优先人群的性传播感染计划进行评估的方案。我们将采用探索性、描述性研究设计，从设施运营、功能、范围、差距、提供服务、性传播感染监测方法和指标等方面对选定初级医疗保健机构的性传播感染计划进行评估。将从南非林波波省的五家初级医疗保健机构中选取 15-20 名性传播感染项目相关人员作为目的性样本。计划评估将使用世界卫生组织的评估核对表工具，这是一种全球公认的、经过验证的工具，由开放式和封闭式问题组成，用于评估性传播感染计划。该工具以其可信度和可靠性著称，确保了研究的有效性。定量数据将采用 STATA 软件（美国德克萨斯州 College Station）第 18 版进行描述性分析，连续变量以平均值和标准差表示，分类变量以比例和百分比表示。p≤0.05 表示统计意义显著。将使用 Atlas.ti 软件（德国柏林工业大学）23.1 版对定性数据进行主题内容分析。研究结果将为加强性传播感染的覆盖面、服务提供和护理联系提供新的方法。

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引用次数: 0

Advances in 3D Inner Ear Reconstruction Software for Cochlear Implants: A Comprehensive Review 用于人工耳蜗植入的 3D 内耳重建软件的进展：全面回顾

IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology

Methods and Protocols

Pub Date : 2024-05-23 DOI: 10.3390/mps7030046

Michael Athanasopoulos, Pinelopi Samara, Ioannis Athanasopoulos

Auditory impairment stands as a pervasive global issue, exerting significant effects on individuals’ daily functioning and interpersonal engagements. Cochlear implants (CIs) have risen as a cutting-edge solution for severe to profound hearing loss, directly stimulating the auditory nerve with electrical signals. The success of CI procedures hinges on precise pre-operative planning and post-operative evaluation, highlighting the significance of advanced three-dimensional (3D) inner ear reconstruction software. Accurate pre-operative imaging is vital for identifying anatomical landmarks and assessing cochlear deformities. Tools like 3D Slicer, Amira and OTOPLAN provide detailed depictions of cochlear anatomy, aiding surgeons in simulating implantation scenarios and refining surgical approaches. Post-operative scans play a crucial role in detecting complications and ensuring CI longevity. Despite technological advancements, challenges such as standardization and optimization persist. This review explores the role of 3D inner ear reconstruction software in patient selection, surgical planning, and post-operative assessment, tracing its evolution and emphasizing features like image segmentation and virtual simulation. It addresses software limitations and proposes solutions, advocating for their integration into clinical practice. Ultimately, this review underscores the impact of 3D inner ear reconstruction software on cochlear implantation, connecting innovation with precision medicine.

听力障碍是一个普遍存在的全球性问题，对个人的日常功能和人际交往产生重大影响。人工耳蜗（CI）直接通过电信号刺激听觉神经，是治疗重度和极重度听力损失的最先进解决方案。CI 手术的成功取决于精确的术前规划和术后评估，这就凸显了先进的三维（3D）内耳重建软件的重要性。精确的术前成像对于确定解剖标志和评估耳蜗畸形至关重要。3D Slicer、Amira 和 OTOPLAN 等工具可提供耳蜗解剖的详细描述，帮助外科医生模拟植入情况并改进手术方法。术后扫描在检测并发症和确保人工耳蜗寿命方面发挥着至关重要的作用。尽管技术不断进步，但标准化和优化等挑战依然存在。本综述探讨了三维内耳重建软件在患者选择、手术规划和术后评估中的作用，追溯了其发展历程，并强调了图像分割和虚拟仿真等功能。它探讨了软件的局限性并提出了解决方案，提倡将其融入临床实践。最后，这篇综述强调了三维内耳重建软件对人工耳蜗植入术的影响，将创新与精准医疗联系起来。

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引用次数: 0