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MUltiparametric Score for Ventilation Discontinuation in Intensive Care Patients: A Protocol for an Observational Study 重症监护患者停止通气的比值评分:观察性研究方案
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-20 DOI: 10.3390/mps7030045
I. Cappellini, Andrea Cardoni, Lorenzo Campagnola, Guglielmo Consales
Background: Mechanical ventilation significantly improves patient survival but is associated with complications, increasing healthcare costs and morbidity. Identifying optimal weaning times is paramount to minimize these risks, yet current methods rely heavily on clinical judgment, lacking specificity. Methods: This study introduces a novel multiparametric predictive score, the MUSVIP (MUltiparametric Score for Ventilation discontinuation in Intensive care Patients), aimed at accurately predicting successful extubation. Conducted at Santo Stefano Hospital’s ICU, this single-center, observational, prospective cohort study will span over 12 months, enrolling adult patients undergoing invasive mechanical ventilation. The MUSVIP integrates variables measured before and during a spontaneous breathing trial (SBT) to formulate a predictive score. Results: Preliminary analyses suggest an Area Under the Curve (AUC) of 0.815 for the MUSVIP, indicating high predictive capacity. By systematically applying this score, we anticipate identifying patients likely to succeed in weaning earlier, potentially reducing ICU length of stay and associated healthcare costs. Conclusion: This study’s findings could significantly influence clinical practices, offering a robust, easy-to-use tool for optimizing weaning processes in ICUs.
背景:机械通气能明显提高患者的存活率,但也会带来并发症,增加医疗成本和发病率。要将这些风险降至最低,确定最佳断流时间至关重要,但目前的方法严重依赖临床判断,缺乏特异性。方法:本研究引入了一种新型多参数预测评分--MUSVIP(重症监护患者中断通气的多参数评分),旨在准确预测成功拔管的时间。这项单中心、观察性、前瞻性队列研究将在圣斯蒂法诺医院重症监护室进行,为期12个月,研究对象为接受有创机械通气的成年患者。MUSVIP 综合了自主呼吸试验 (SBT) 前和试验过程中测量的变量,以制定预测评分。研究结果初步分析表明,MUSVIP 的曲线下面积 (AUC) 为 0.815,显示出较高的预测能力。通过系统地应用该评分,我们预计能识别出更早成功断奶的患者,从而缩短重症监护室的住院时间并降低相关医疗费用。结论:这项研究的结果将对临床实践产生重大影响,为优化重症监护病房的断奶流程提供了一个强大、易用的工具。
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引用次数: 0
Pole Dancing-Specific Muscle Strength: Development and Reliability of a Novel Assessment Protocol 钢管舞专用肌力:新型评估方案的开发与可靠性
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-18 DOI: 10.3390/mps7030044
Despoina Ignatoglou, Achilleas Paliouras, E. Paraskevopoulos, N. Strimpakos, Paraskevi Bilika, Maria Papandreou, Eleni Kapreli
Background: Pole dancing is a physically demanding sport that combines dance and acrobatic movements on a vertical pole. Despite its highly growing popularity, there is currently limited research in the field. The aim of this study was to create and evaluate a strength assessment protocol for athletes in pole dancing, with a specific focus on functional positions on the pole. Methods: Thirty-two female pole dancing athletes participated in this study. Maximal voluntary isometric contractions (MVIC) were measured at three different sport-specific positions on the pole (shoulder abduction and adduction, and hip adduction), on two separate days (test and re-test) with a five to seven day interval between them. A hand-held dynamometer (Activ5- Activbody) stabilized on the pole was used for this study. Results: The intra-session reliability was good to excellent for all sports-specific positions and for both sides of the body, across all different movements (ICC = 0.837–0.960, SEM = 5.02Kg-2.24Kg, and SDD = 27.46%-14.92%). Slightly better results were found regarding inter-session reliability (ICC = 0.927–0.970, SEM = 3.72Kg-1.97Kg, and SDD = 22.86%-15.19%). There was not a statistically significant difference between the MVICs between the left and right or dominant and non-dominant side in shoulder abduction (p = 0.105) and hip adduction (p = 0.282), in contrast to shoulder adduction (p = 0.00). Conclusion: The strength assessment protocol developed in the current study has proven to be a reliable and functional tool, with the potential for utilization in clinical practice as part of objective strength testing. Further studies are needed in order to expand the protocol to other muscle groups and positions and to generalize the results in all pole dancing populations such as male athletes.
背景介绍钢管舞是一项耗费体力的运动,它将舞蹈和杂技动作结合在一根垂直的钢管上。尽管钢管舞越来越受欢迎,但目前该领域的研究却十分有限。本研究的目的是为钢管舞运动员制定并评估一项力量评估方案,重点关注钢管上的功能性姿势。研究方法32 名女性钢管舞运动员参与了这项研究。在钢管上三个不同的运动特定位置(肩部外展、内收和髋部内收)测量最大自主等长收缩(MVIC),分别在两天内进行(测试和复测),中间间隔五到七天。本研究使用的是稳定在杆上的手持式测力计(Activ5- Activbody)。研究结果对于所有运动特定姿势和身体两侧的所有不同动作,会期内的可靠性都达到了良好至优秀(ICC = 0.837-0.960,SEM = 5.02千克-2.24千克,SDD = 27.46%-14.92%)。两次测验之间的可靠性稍好一些(ICC = 0.927-0.970, SEM = 3.72Kg-1.97Kg, SDD = 22.86%-15.19%)。在肩关节外展(p = 0.105)和髋关节内收(p = 0.282)方面,左右侧或优势侧与非优势侧的 MVICs 之间没有统计学意义上的显著差异,而肩关节内收(p = 0.00)则相反。结论当前研究中开发的力量评估方案已被证明是一种可靠且实用的工具,有望在临床实践中作为客观力量测试的一部分加以应用。还需要进一步研究,以便将该方案扩展到其他肌肉群和位置,并将结果推广到所有钢管舞人群,如男性运动员。
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引用次数: 0
Isolation of Intestinal Macrophage Subpopulations for High-Quality Total RNA Purification in Zebrafish 分离斑马鱼肠道巨噬细胞亚群以进行高质量总 RNA 纯化
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-17 DOI: 10.3390/mps7030043
Yalén Del Río-Jay, Audrey Barthelaix, Cristian Reyes-Martínez, Christophe Duperray, Camila J. Solis-Cascante, Yessia Hidalgo, Patricia Luz-Crawford, Farida Djouad, Carmen G. Feijoo
Intestinal macrophages have been poorly studied in fish, mainly due to the lack of specific molecular markers for their identification and isolation. To address this gap, using the zebrafish Tg(mpeg1:EGFP) transgenic line, we developed a fluorescence-activated cell sorting strategy (FACS) that allows us to isolate different intestinal macrophage subpopulations, based on GFP expression and morphological differences. Also, we achieved the purification of high-quality total RNA from each population to perform transcriptomic analysis. The complete strategy comprises three steps, including intestine dissection and tissue dissociation, the isolation of each intestinal macrophage population via FACS, and the extraction of total RNA. To be able to characterize molecularly different macrophage subpopulations and link them to their functional properties will allow us to unravel intestinal macrophage biology.
对鱼类肠道巨噬细胞的研究很少,主要原因是缺乏识别和分离它们的特异性分子标记。为了填补这一空白,我们利用斑马鱼 Tg(mpeg1:EGFP)转基因品系,开发了一种荧光激活细胞分选策略(FACS),可以根据 GFP 表达和形态差异分离出不同的肠巨噬细胞亚群。此外,我们还从每个亚群中纯化出高质量的总 RNA,以进行转录组分析。完整的策略包括三个步骤,包括肠道解剖和组织解离、通过 FACS 分离每个肠道巨噬细胞群以及提取总 RNA。通过分子鉴定不同的巨噬细胞亚群,并将它们与其功能特性联系起来,将使我们能够揭示肠巨噬细胞的生物学特性。
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引用次数: 0
Syrian Hamsters Model Does Not Reflect Human-Like Disease after Aerosol Exposure to Encephalitic Alphaviruses 叙利亚仓鼠模型在气溶胶暴露于脑阿尔法病毒后并不反映类似人类的疾病
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-15 DOI: 10.3390/mps7030042
Christina L. Gardner, Rebecca A. Erwin-Cohen, Bridget S. Lewis, Russell R. Bakken, Shelley P. Honnold, Pamela J. Glass, Crystal W. Burke
Venezuelan (VEE), eastern (EEE), and western (WEE) equine encephalitis viruses are encephalitic New World alphaviruses that cause periodic epizootic and epidemic outbreaks in horses and humans that may cause severe morbidity and mortality. Currently there are no FDA-licensed vaccines or effective antiviral therapies. Each year, there are a limited number of human cases of encephalitic alphaviruses; thus, licensure of a vaccine or therapeutic would require approval under the FDA animal rule. Approval under the FDA animal rule requires the disease observed in the animal model to recapitulate what is observed in humans. Currently, initial testing of vaccines and therapeutics is performed in the mouse model. Unfortunately, alphavirus disease manifestations in a mouse do not faithfully recapitulate human disease; the VEEV mouse model is lethal whereas in humans VEEV is rarely lethal. In an effort to identify a more appropriate small animal model, we evaluated hamsters in an aerosol exposure model of encephalitic alphavirus infection. The pathology, lethality, and viremia observed in the infected hamsters was inconsistent with what is observed in NHP models and humans. These data suggest that hamsters are not an appropriate model for encephalitic alphaviruses to test vaccines or potential antiviral therapies.
委内瑞拉马脑炎病毒(VEE)、东部马脑炎病毒(EEE)和西部马脑炎病毒(WEE)都是新世界α-病毒,它们会在马和人体内引起周期性的流行病暴发,可能导致严重的发病和死亡。目前,美国食品和药物管理局尚未批准使用疫苗或有效的抗病毒疗法。每年,人类感染脑炎阿尔巴病毒的病例数量有限;因此,疫苗或疗法的许可需要获得美国食品及药物管理局动物规则的批准。根据美国食品及药物管理局的动物规则进行审批,要求在动物模型中观察到的疾病能够再现在人类身上观察到的情况。目前,疫苗和疗法的初步测试是在小鼠模型中进行的。不幸的是,阿尔法病毒在小鼠中的疾病表现并不能忠实地再现人类疾病;VEEV 小鼠模型是致命的,而在人类中 VEEV 很少致命。为了确定一种更合适的小动物模型,我们对仓鼠进行了脑炎阿尔卑斯病毒感染气溶胶暴露模型的评估。在受感染的仓鼠身上观察到的病理变化、致死率和病毒血症与在 NHP 模型和人类身上观察到的不一致。这些数据表明,仓鼠不是测试疫苗或潜在抗病毒疗法的合适的脑炎α-病毒模型。
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引用次数: 0
Methodological Considerations Regarding the Quantification of DNA Impurities in the COVID-19 mRNA Vaccine Comirnaty® 有关 COVID-19 mRNA 疫苗 Comirnaty® 中 DNA 杂质定量的方法学考虑因素
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-08 DOI: 10.3390/mps7030041
B. König, Jürgen O. Kirchner
DNA impurities can impact the safety of genetically engineered pharmaceuticals; thus, a specific limit value must be set for them during marketing authorisation. This particularly applies to mRNA vaccines, as large quantities of DNA templates are used for their production. Furthermore, when quantifying the total DNA content in the final product, we must observe that, in addition to the mRNA active ingredient, DNA impurities are also encased in lipid nanoparticles and are therefore difficult to quantify. In fact, the manufacturer of the mRNA vaccine Comirnaty (BioNTech/Pfizer) only measures DNA impurities in the active substance by means of a quantitative polymerase chain reaction (qPCR), whose DNA target sequence is less than just 1% of the originally added DNA template. This means that no direct DNA quantification takes place, and compliance with the limit value for DNA contamination is only estimated from the qPCR data using mathematical extrapolation methods. However, it is also possible to dissolve the lipid nanoparticles with a detergent to directly measure DNA contamination in the final product by using fluorescence spectroscopic methods. Experimental testing of this approach confirms that reliable values can be obtained in this way.
DNA 杂质会影响基因工程药品的安全性,因此在上市许可过程中必须为其设定特定的限值。这一点尤其适用于 mRNA 疫苗,因为生产过程中会使用大量 DNA 模板。此外,在量化最终产品中的 DNA 总含量时,我们必须注意到,除了 mRNA 活性成分外,DNA 杂质也被包裹在脂质纳米颗粒中,因此很难量化。事实上,mRNA 疫苗制造商 Comirnaty(BioNTech/辉瑞)只通过定量聚合酶链反应(qPCR)来测量活性物质中的 DNA 杂质,其 DNA 目标序列只占最初添加的 DNA 模板的不到 1%。这意味着不需要对 DNA 进行直接定量,只需通过数学推断方法从 qPCR 数据中估算出是否符合 DNA 污染的限值。不过,也可以用洗涤剂溶解脂质纳米粒子,利用荧光光谱法直接测量最终产品中的 DNA 污染情况。对这种方法的实验测试证实,这种方法可以获得可靠的数值。
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引用次数: 0
Quantifying mRNA in Highly Degraded Fixed Tissues by Nanostring Technology: A Comparative Study 利用纳米试管技术量化高度降解固定组织中的 mRNA:比较研究
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-07 DOI: 10.3390/mps7030040
E. Azzalini, Barbara Di Stefano, Vincenzo Canzonieri, Tiziana Venesio, Umberto Miglio, C. Marchiò, A. Sapino, C. Previderè, P. Fattorini, Serena Bonin
Archive tissues are the most available source of human tissues useful for molecular analysis in translational research. The main issues for those specimens are the modification and degradation of biomolecules, namely proteins, DNA, and RNA. In the last decade, several high-throughput analytical methods have been applied to archive tissues. Although histological tissues are fixed in neutral-buffered formalin nowadays, in the recent past, Bouin’s solution was also used in tissue processing. The present study aims to investigate the feasibility of nCounter Nanostring hybridization in quantifying mRNA in highly degraded samples, such as Bouin’s fixed and paraffin-embedded (BFPE) tissues, in comparison to the standard formalin-fixed and paraffin-embedded (FFPE) tissues as a source of RNA. A total of 16 paraffin-embedded tissue blocks from eight patients were analyzed (8 were FFPE and 8 were BEPE). Nanostring technology was applied to 300 ng of each RNA sample, whereas 360 ng of the same templates were retrotranscribed and submitted to qPCR and ddPCR. Our results show that the Nanostring technology outperforms the reference methods (ddPCR and qPCR) in detecting target mRNA in FFPE and BFPE samples. However, even Nanostring technology does not escape the limitation imposed by the degradation of the RNA templates, which could lead to misleading conclusions on the gene expression level.
档案组织是转化研究中进行分子分析最有用的人体组织来源。这些标本的主要问题是生物大分子(即蛋白质、DNA 和 RNA)的修饰和降解。近十年来,一些高通量分析方法已被应用于档案组织。虽然现在组织学组织是用中性缓冲福尔马林固定的,但在最近的过去,布因溶液也被用于组织处理。本研究旨在探讨 nCounter 纳米环杂交技术与作为 RNA 来源的标准福尔马林固定和石蜡包埋(FFPE)组织相比,量化高度降解样本(如布氏固定和石蜡包埋(BFPE)组织)中 mRNA 的可行性。共分析了 8 位患者的 16 块石蜡包埋组织块(8 块为 FFPE,8 块为 BEPE)。对每个 RNA 样本的 300 纳克应用了纳米环技术,而对 360 纳克的相同模板进行了逆转录并进行了 qPCR 和 ddPCR。结果表明,在检测 FFPE 和 BFPE 样品中的目标 mRNA 方面,Nanostring 技术优于参考方法(ddPCR 和 qPCR)。然而,即使是纳米环技术也无法摆脱 RNA 模板降解的限制,这可能会导致对基因表达水平得出误导性结论。
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引用次数: 0
A Protocol for Comprehensive Analysis of Gait in Individuals with Incomplete Spinal Cord Injury 综合分析不完全脊髓损伤患者步态的规程
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-04 DOI: 10.3390/mps7030039
Emelie Butler Forslund, Minh Tat Nhat Truong, Ruoli Wang, Åke Seiger, E. Gutierrez-Farewik
This is a protocol for comprehensive analysis of gait and affecting factors in individuals with incomplete paraplegia due to spinal cord injury (SCI). A SCI is a devastating event affecting both sensory and motor functions. Due to better care, the SCI population is changing, with a greater proportion retaining impaired ambulatory function. Optimizing ambulatory function after SCI remains challenging. To investigate factors influencing optimal ambulation, a multi-professional research project was grounded with expertise from clinical rehabilitation, neurophysiology, and biomechanical engineering from Karolinska Institutet, the Spinalis Unit at Aleris Rehab Station (Sweden’s largest center for specialized neurorehabilitation), and the Promobilia MoveAbility Lab at KTH Royal Institute of Technology. Ambulatory adults with paraplegia will be consecutively invited to participate. Muscle strength, sensitivity, and spasticity will be assessed, and energy expenditure, 3D movements, and muscle function (EMG) during gait and submaximal contractions will be analyzed. Innovative computational modeling and data-driven analyses will be performed, including the identification of clusters of similar movement patterns among the heterogeneous population and analyses that study the link between complex sensorimotor function and movement performance. These results may help optimize ambulatory function for persons with SCI and decrease the risk of secondary conditions during gait with a life-long perspective.
这是一份全面分析脊髓损伤(SCI)导致的不完全截瘫患者步态及其影响因素的方案。脊髓损伤是一种破坏性事件,会影响感官和运动功能。由于护理水平的提高,SCI 患者正在发生变化,越来越多的人保留了受损的活动功能。优化 SCI 后的行走功能仍然是一项挑战。为了研究影响最佳活动能力的因素,卡罗林斯卡医学院、Aleris 康复站(瑞典最大的专业神经康复中心)脊柱单元和瑞典皇家理工学院 Promobilia 运动能力实验室的临床康复、神经生理学和生物机械工程专家共同开展了一项跨专业研究项目。将连续邀请患有截瘫、行动不便的成年人参加。将对肌肉力量、敏感性和痉挛性进行评估,并对步态和亚最大收缩时的能量消耗、三维运动和肌肉功能(肌电图)进行分析。还将进行创新的计算建模和数据驱动分析,包括识别异质人群中相似运动模式的群组,以及研究复杂感觉运动功能与运动表现之间联系的分析。这些结果可能有助于优化 SCI 患者的活动功能,并从终生的角度降低步态过程中继发病症的风险。
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引用次数: 0
Chromatographic Methods for the Determination of Glyphosate in Cereals together with a Discussion of Its Occurrence, Accumulation, Fate, Degradation, and Regulatory Status 测定谷物中草甘膦含量的色谱法及其发生、积累、归宿、降解和监管现状的讨论
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-02 DOI: 10.3390/mps7030038
M. Masci, R. Caproni, Teresina Nevigato
The European Union’s recent decision to renew the authorization for the use of glyphosate until 15 December 2033 has stimulated scientific discussion all around the world regarding its toxicity or otherwise for humans. Glyphosate is a chemical of which millions of tons have been used in the last 50 years worldwide to dry out weeds in cultivated fields and greenhouses and on roadsides. Concern has been raised in many areas about its possible presence in the food chain and its consequent adverse effects on health. Both aspects that argue in favor of toxicity and those that instead may indicate limited toxicity of glyphosate are discussed here. The widespread debate that has been generated requires further investigations and field measurements to understand glyphosate’s fate once dispersed in the environment and its concentration in the food chain. Hence, there is a need for validated analytical methods that are available to analysts in the field. In the present review, methods for the analytical determination of glyphosate and its main metabolite, AMPA, are discussed, with a specific focus on chromatographic techniques applied to cereal products. The experimental procedures are explained in detail, including the cleanup, derivatization, and instrumental conditions, to give the laboratories involved enough information to proceed with the implementation of this line of analysis. The prevalent chromatographic methods used are LC-MS/MS, GC-MS/SIM, and GC-MS/MS, but sufficient indications are also given to those laboratories that wish to use the better performing high-resolution MS or the simpler HPLC-FLD, HPLC-UV, GC-NPD, and GC-FPD techniques for screening purposes. The concentrations of glyphosate from the literature measured in wheat, corn, barley, rye, oats, soybean, and cereal-based foods are reported, together with its regulatory status in various parts of the world and its accumulation mechanism. As for its accumulation in cereals, the available data show that glyphosate tends to accumulate more in wholemeal flours than in refined ones, that its concentration in the product strictly depends on the treatment period (the closer it is to the time of harvesting, the higher the concentration), and that in cold climates, the herbicide tends to persist in the soil for a long time.
欧盟最近决定将草甘膦的使用授权延长至 2033 年 12 月 15 日,这引发了全世界关于草甘膦对人类是否有毒的科学讨论。草甘膦是一种化学品,在过去的 50 年里,全世界使用了数百万吨草甘膦来清除耕地、温室和路边的杂草。许多地区都对草甘膦可能进入食物链以及由此对健康产生的不利影响表示担忧。这里既讨论了草甘膦的毒性,也讨论了草甘膦的有限毒性。引起的广泛争论需要进一步调查和实地测量,以了解草甘膦在环境中扩散后的命运及其在食物链中的浓度。因此,需要有可供实地分析人员使用的有效分析方法。在本综述中,讨论了分析测定草甘膦及其主要代谢物 AMPA 的方法,特别侧重于应用于谷物产品的色谱技术。文中详细解释了实验程序,包括净化、衍生化和仪器条件,以便为相关实验室提供足够的信息,继续实施这一分析方法。常用的色谱分析方法有 LC-MS/MS、GC-MS/SIM 和 GC-MS/MS,但也为那些希望使用性能更好的高分辨率 MS 或更简单的 HPLC-FLD、HPLC-UV、GC-NPD 和 GC-FPD 技术进行筛选的实验室提供了充分的说明。报告了文献中测得的草甘膦在小麦、玉米、大麦、黑麦、燕麦、大豆和谷类食品中的浓度,以及草甘膦在世界各地的监管状况和积累机制。关于草甘膦在谷物中的积累,现有数据表明,草甘膦在全麦面粉中的积累往往多于在精制面粉中的积累,其在产品中的浓度严格取决于处理时间(越接近收获时间,浓度越高),在寒冷的气候条件下,除草剂往往在土壤中长期存在。
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引用次数: 0
Simplifying Data Analysis in Biomedical Research: An Automated, User-Friendly Tool 简化生物医学研究中的数据分析:自动化、用户友好型工具
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-24 DOI: 10.3390/mps7030036
Rúben Araújo, Luís Ramalhete, Ana Viegas, Cristiana P Von Rekowski, Tiago A H Fonseca, C. Calado, Luís Bento
Robust data normalization and analysis are pivotal in biomedical research to ensure that observed differences in populations are directly attributable to the target variable, rather than disparities between control and study groups. ArsHive addresses this challenge using advanced algorithms to normalize populations (e.g., control and study groups) and perform statistical evaluations between demographic, clinical, and other variables within biomedical datasets, resulting in more balanced and unbiased analyses. The tool’s functionality extends to comprehensive data reporting, which elucidates the effects of data processing, while maintaining dataset integrity. Additionally, ArsHive is complemented by A.D.A. (Autonomous Digital Assistant), which employs OpenAI’s GPT-4 model to assist researchers with inquiries, enhancing the decision-making process. In this proof-of-concept study, we tested ArsHive on three different datasets derived from proprietary data, demonstrating its effectiveness in managing complex clinical and therapeutic information and highlighting its versatility for diverse research fields.
稳健的数据归一化和分析在生物医学研究中至关重要,可确保观察到的人群差异直接归因于目标变量,而不是对照组和研究组之间的差异。ArsHive 利用先进的算法对人群(如对照组和研究组)进行归一化处理,并对生物医学数据集中的人口、临床和其他变量进行统计评估,从而使分析结果更加平衡、公正。该工具的功能还可扩展到全面的数据报告,在保持数据集完整性的同时,阐明数据处理的效果。此外,ArsHive 还配备了 A.D.A.(自主数字助理),它采用 OpenAI 的 GPT-4 模型来协助研究人员进行查询,从而加强决策过程。在这项概念验证研究中,我们在源自专有数据的三个不同数据集上对 ArsHive 进行了测试,证明了它在管理复杂的临床和治疗信息方面的有效性,并突出了它在不同研究领域的通用性。
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引用次数: 0
Dedicated Protocol for Ultrastructural Analysis of Farmed Rainbow Trout (Oncorhynchus mykiss) Tissues with Red Mark Syndrome: The Skin—Part One 养殖虹鳟鱼(Oncorhynchus mykiss)红印综合征组织超微结构分析专用方案:皮肤--第一部分
IF 2.4 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-24 DOI: 10.3390/mps7030037
Diana Torge, S. Bernardi, Giulia Ciciarelli, Guido Macchiarelli, S. Bianchi
The present study aims to provide a specific protocol for transmission electron microscopy of a sample of skin of rainbow trout affected by red mark syndrome (RMS). The red mark syndrome is a skin disease that affects the rainbow trout (Oncorhynchus mykiss). The disease, probably due to the Midichloria-like organism infection, is not lethal, but morbidity can reach up to 60%, leading to significant economic impact associated with the downgrading of the commercial product, increased labor, and susceptibility to secondary infections. The ultrastructure analyses allowed an earlier study to identify the presence of scattered microorganisms characterized by an oval shape, mainly in the cytoplasm of the cells. The protocol developed in this study will be instrumental in visualizing the ultrastructure of the microorganism, which is probably responsible for red mark syndrome infection.
本研究旨在为受红印综合征(RMS)影响的虹鳟鱼皮肤样本的透射电子显微镜检查提供一个具体的方案。红印综合征是一种影响虹鳟鱼(Oncorhynchus mykiss)的皮肤病。这种疾病可能是由类敌百虫感染引起的,虽然不致命,但发病率高达 60%,会导致商品降级、劳动力增加以及易继发感染等重大经济影响。通过超微结构分析,早先的一项研究确定了散在微生物的存在,其特点是呈椭圆形,主要存在于细胞的细胞质中。本研究制定的方案将有助于观察微生物的超微结构,这可能是感染红印综合征的原因。
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