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Exploring the multidimensional nature of repetitive and restricted behaviors and interests (RRBI) in autism: neuroanatomical correlates and clinical implications. 探索自闭症中重复性和限制性行为和兴趣(RRBI)的多维本质:神经解剖学相关性和临床意义。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-11-27 DOI: 10.1186/s13229-023-00576-z
Aline Lefebvre, Nicolas Traut, Amandine Pedoux, Anna Maruani, Anita Beggiato, Monique Elmaleh, David Germanaud, Anouck Amestoy, Myriam Ly-Le Moal, Christopher Chatham, Lorraine Murtagh, Manuel Bouvard, Marianne Alisson, Marion Leboyer, Thomas Bourgeron, Roberto Toro, Guillaume Dumas, Clara Moreau, Richard Delorme

Background: Repetitive and restricted behaviors and interests (RRBI) are core symptoms of autism with a complex entity and are commonly categorized into 'motor-driven' and 'cognitively driven'. RRBI symptomatology depends on the individual's clinical environment limiting the understanding of RRBI physiology, particularly their associated neuroanatomical structures. The complex RRBI heterogeneity needs to explore the whole RRBI spectrum by integrating the clinical context [autistic individuals, their relatives and typical developing (TD) individuals]. We hypothesized that different RRBI dimensions would emerge by exploring the whole spectrum of RRBI and that these dimensions are associated with neuroanatomical signatures-involving cortical and subcortical areas.

Method: A sample of 792 individuals composed of 267 autistic subjects, their 370 first-degree relatives and 155 TD individuals was enrolled in the study. We assessed the whole patterns of RRBI in each individual by using the Repetitive Behavior Scale-Revised and the Yale-Brown Obsessive Compulsive Scale. We estimated brain volumes using MRI scanner for a subsample of the subjects (n = 152, 42 ASD, 89 relatives and 13 TD). We first investigated the dimensionality of RRBI by performing a principal component analysis on all items of these scales and included all the sampling population. We then explored the relationship between RRBI-derived factors with brain volumes using linear regression models.

Results: We identified 3 main factors (with 30.3% of the RRBI cumulative variance): Factor 1 (FA1, 12.7%) reflected mainly the 'motor-driven' RRBI symptoms; Factor 2 and 3 (respectively, 8.8% and 7.9%) gathered mainly Y-BOCS related items and represented the 'cognitively driven' RRBI symptoms. These three factors were significantly associated with the right/left putamen volumes but with opposite effects: FA1 was negatively associated with an increased volume of the right/left putamen conversely to FA2 and FA3 (all uncorrected p < 0.05). FA1 was negatively associated with the left amygdala (uncorrected p < 0.05), and FA2 was positively associated with the left parietal structure (uncorrected p = 0.001).

Conclusion: Our results suggested 3 coherent RRBI dimensions involving the putamen commonly and other structures according to the RRBI dimension. The exploration of the putamen's integrative role in RSBI needs to be strengthened in further studies.

背景:重复性和限制性行为和兴趣(RRBI)是具有复杂实体的自闭症的核心症状,通常分为“运动驱动”和“认知驱动”。RRBI的症状取决于个体的临床环境,这限制了对RRBI生理学的理解,特别是其相关的神经解剖结构。复杂的RRBI异质性需要通过整合临床背景[自闭症个体、其亲属和典型发展(TD)个体]来探索整个RRBI谱。我们假设,通过探索RRBI的全谱,会出现不同的RRBI维度,并且这些维度与涉及皮层和皮层下区域的神经解剖学特征相关。方法:选取267名自闭症患者及其370名一级亲属和155名TD患者共792人进行研究。我们使用重复行为量表和耶鲁-布朗强迫症量表来评估每个个体的RRBI的整体模式。我们使用MRI扫描仪估算了受试者亚样本的脑容量(n = 152, 42名ASD, 89名亲属和13名TD)。我们首先通过对这些量表的所有项目进行主成分分析来调查RRBI的维度,并包括所有的抽样人口。然后,我们使用线性回归模型探讨了rrbi衍生因素与脑容量之间的关系。结果:我们确定了3个主要因素(占RRBI累积方差的30.3%):因素1 (FA1, 12.7%)主要反映“马达驱动”的RRBI症状;因子2和因子3(分别为8.8%和7.9%)主要收集Y-BOCS相关项目,代表“认知驱动”的RRBI症状。这三个因素与左右壳核体积显著相关,但具有相反的作用:FA1与FA2和FA3相反,与左右壳核体积增加负相关(均未校正)。结论:我们的结果表明,3个一致的RRBI维度通常涉及壳核,并根据RRBI维度涉及其他结构。壳核在RSBI中的综合作用有待进一步的研究。
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引用次数: 0
Increased cerebral lactate levels in adults with autism spectrum disorders compared to non-autistic controls: a magnetic resonance spectroscopy study. 与非自闭症对照相比,自闭症谱系障碍成人脑乳酸水平升高:磁共振波谱研究。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-11-17 DOI: 10.1186/s13229-023-00577-y
Simon Maier, Kathrin Nickel, Thomas Lange, Georg Oeltzschner, Michael Dacko, Dominique Endres, Kimon Runge, Anke Schumann, Katharina Domschke, Michalis Rousos, Ludger Tebartz van Elst

Introduction: Autism spectrum disorder (ASD) encompasses a heterogeneous group with varied phenotypes and etiologies. Identifying pathogenic subgroups could facilitate targeted treatments. One promising avenue is investigating energy metabolism, as mitochondrial dysfunction has been implicated in a subgroup of ASD. Lactate, an indicator of energy metabolic anomalies, may serve as a potential biomarker for this subgroup. This study aimed to examine cerebral lactate (Lac+) levels in high-functioning adults with ASD, hypothesizing elevated mean Lac+ concentrations in contrast to neurotypical controls (NTCs).

Materials and methods: Magnetic resonance spectroscopy (MRS) was used to study cerebral Lac+ in 71 adults with ASD and NTC, focusing on the posterior cingulate cortex (PCC). After quality control, 64 ASD and 58 NTC participants remained. Lac+ levels two standard deviations above the mean of the control group were considered elevated.

Results: Mean PCC Lac+ levels were significantly higher in the ASD group than in the NTC group (p = 0.028; Cohen's d = 0.404), and 9.4% of the ASD group had elevated levels as compared to 0% of the NTCs (p = 0.029). No significant correlation was found between blood serum lactate levels and MRS-derived Lac+ levels.

Limitations: A cautious interpretation of our results is warranted due to a p value of 0.028. In addition, a higher than anticipated proportion of data sets had to be excluded due to poor spectral quality.

Conclusion: This study confirms the presence of elevated cerebral Lac+ levels in a subgroup of adults with ASD, suggesting the potential of lactate as a biomarker for mitochondrial dysfunction in a subgroup of ASD. The lower-than-expected prevalence (20% was expected) and moderate increase require further investigation to elucidate the underlying mechanisms and relationships with mitochondrial function.

简介:自闭症谱系障碍(ASD)包括具有不同表型和病因的异质群体。确定致病亚群有助于有针对性的治疗。一个有希望的途径是研究能量代谢,因为线粒体功能障碍与ASD的一个亚群有关。乳酸作为能量代谢异常的指标,可能作为该亚群的潜在生物标志物。本研究旨在检测高功能成人ASD患者的脑乳酸(Lac+)水平,并假设与神经正常对照组(ntc)相比,Lac+的平均浓度升高。材料与方法:采用磁共振波谱法(MRS)对71例成人ASD合并NTC患者的脑组织Lac+进行研究,重点观察后扣带皮层(PCC)。质量控制后,64名ASD和58名NTC参与者保留。Lac+水平高于对照组平均值两个标准差被认为升高。结果:ASD组平均PCC Lac+水平显著高于NTC组(p = 0.028;Cohen’s d = 0.404),与0%的ntc相比,9.4%的ASD组的水平升高(p = 0.029)。血清乳酸水平与mrs衍生Lac+水平无显著相关性。局限性:由于p值为0.028,对我们的结果进行谨慎的解释是必要的。此外,由于光谱质量差,必须排除比预期比例更高的数据集。结论:本研究证实了成人ASD亚组中存在升高的脑Lac+水平,提示乳酸盐可能作为ASD亚组中线粒体功能障碍的生物标志物。低于预期的患病率(预计为20%)和适度增长需要进一步调查,以阐明潜在的机制及其与线粒体功能的关系。
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引用次数: 0
Hyperthermia elevates brain temperature and improves behavioural signs in animal models of autism spectrum disorder. 在自闭症谱系障碍动物模型中,热疗可提高脑温度并改善行为体征。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-11-15 DOI: 10.1186/s13229-023-00569-y
Ana Belen Lopez-Rodriguez, Carol L Murray, John Kealy, Clodagh Towns, Andrew Roche, Arshed Nazmi, Michelle Doran, John P Lowry, Colm Cunningham

Background: Autism spectrum disorders (ASD) are predominantly neurodevelopmental and largely genetically determined. However, there are human data supporting the idea that fever can improve symptoms in some individuals, but those data are limited and there are almost no data to support this from animal models. We aimed to test the hypothesis that elevated body temperature would improve function in two animal models of ASD.

Methods: We used a 4 h whole-body hyperthermia (WBH) protocol and, separately, systemic inflammation induced by bacterial endotoxin (LPS) at 250 µg/kg, to dissociate temperature and inflammatory elements of fever in two ASD animal models: C58/J and Shank3B- mice. We used one- or two-way ANOVA and t-tests with normally distributed data and Kruskal-Wallis or Mann-Whitney with nonparametric data. Post hoc comparisons were made with a level of significance set at p < 0.05. For correlation analyses, data were adjusted by a linear regression model.

Results: Only LPS induced inflammatory signatures in the brain while only WBH produced fever-range hyperthermia. WBH reduced repetitive behaviours and improved social interaction in C58/J mice and significantly reduced compulsive grooming in Shank3B- mice. LPS significantly suppressed most activities over 5-48 h.

Limitations: We show behavioural, cellular and molecular changes, but provide no specific mechanistic explanation for the observed behavioural improvements.

Conclusions: The data are the first, to our knowledge, to demonstrate that elevated body temperature can improve behavioural signs in 2 distinct ASD models. Given the developmental nature of ASD, evidence that symptoms may be improved by environmental perturbations indicates possibilities for improving function in these individuals. Since experimental hyperthermia in patients would carry significant risks, it is now essential to pursue molecular mechanisms through which hyperthermia might bring about the observed benefits.

背景:自闭症谱系障碍(ASD)主要是神经发育和很大程度上由遗传决定的。然而,有人类数据支持发烧可以改善某些人症状的观点,但这些数据有限,而且几乎没有来自动物模型的数据支持这一观点。我们的目的是在两种ASD动物模型中验证体温升高会改善功能的假设。方法:采用4 h全身热疗法(WBH)和250µg/kg细菌内毒素(LPS)诱导的全身性炎症,分别分离C58/J和Shank3B-两种ASD动物模型小鼠的温度和发热炎症因子。我们对正态分布数据使用单因素或双因素方差分析和t检验,对非参数数据使用Kruskal-Wallis或Mann-Whitney检验。事后比较的显著性水平设为p。结果:只有LPS在大脑中诱导炎症特征,而只有白头菌产生发热范围的高温。WBH减少了C58/J小鼠的重复行为并改善了社会互动,显著减少了Shank3B-小鼠的强迫性梳理。局限性:我们显示了行为、细胞和分子的变化,但没有提供观察到的行为改善的具体机制解释。结论:据我们所知,这些数据是第一次证明体温升高可以改善两种不同ASD模型的行为体征。考虑到ASD的发育性质,环境扰动可能改善症状的证据表明,改善这些个体的功能是可能的。由于对患者进行实验性热疗会带来重大风险,因此现在有必要探索热疗可能带来观察到的益处的分子机制。
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引用次数: 0
Tau reduction attenuates autism-like features in Fmr1 knockout mice. Tau减少可减弱Fmr1基因敲除小鼠的自闭症样特征。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-11-07 DOI: 10.1186/s13229-023-00574-1
Shanshan Zhao, Xiangyu Jiang, Linkun Han, Yiru Jiang, Yong Wang, Jian Meng, Xiang Zhu, Xian Zhang, Hong Luo, Yun-Wu Zhang

Background: Fragile X syndrome (FXS) is a leading cause of autism spectrum disorder (ASD) and resulted from a loss of the FMR1-encoded fragile X messenger ribonucleoprotein 1 (FMRP) protein due to large CGG repeat expansions in the promoter region of the FMR1 gene. The microtubule-associated protein Tau is a promising target for Tauopathic diseases and our preliminary study found that Tau protein levels were increased in the brain of Fmr1 knockout (KO) mice, a model of FXS. However, whether Tau reduction can prevent autism-like features in Fmr1 KO mice and become a novel strategy for FXS treatment remain unknown.

Methods: Tau was genetically reduced in Fmr1 KO mice through crossing Fmr1± female mice with Mapt± male mice. The male offspring with different genotypes were subjected to various autism-related behavioral tests, RNA sequencing, and biochemical analysis. Fmr1 KO male mice were treated with Tau-targeting antisense oligonucleotide (ASO) and then subjected to behavioral tests and biochemical analysis.

Results: Tau expression was increased in the cortex of Fmr1 KO mice. Genetically reducing Tau prevented social defects, stereotyped and repetitive behavior, and spine abnormality in Fmr1 KO mice. Tau reduction also reversed increased periodic activity and partially rescued Per1 expression reduction in Fmr1 KO mice. Moreover, Tau reduction reversed compromised P38/MAPK signaling in Fmr1 KO mice. Finally, Tau-targeting ASO also effectively alleviated autism-like phenotypes and promoted P38/MAPK signaling in Fmr1 KO mice.

Limitations: Our study is limited to male mice, in agreement with the higher incidence of FXS in males than females. Whether Tau reduction also exerts protection in females deserves further scrutiny. Moreover, although Tau reduction rescues impaired P38/MAPK signaling in Fmr1 KO mice, whether this is the responsible molecular mechanism requires further determination.

Conclusion: Our data indicate that Tau reduction prevents autism-like phenotypes in Fmr1 KO mice. Tau may become a new target for FXS treatment.

背景:脆性X综合征(FXS)是自闭症谱系障碍(ASD)的主要原因,是由于FMR1基因启动子区CGG重复序列大量扩增,导致FMR1编码的脆性X信使核糖核蛋白1(FMRP)蛋白缺失。微管相关蛋白Tau是Tau病的一个有前途的靶点,我们的初步研究发现,Fmr1敲除(KO)小鼠(FXS模型)大脑中的Tau蛋白水平增加。然而,Tau减少是否可以预防Fmr1-KO小鼠的自闭症样特征,并成为FXS治疗的一种新策略,目前尚不清楚。方法:通过Fmr1±雌性小鼠与Mapt±雄性小鼠的杂交,在Fmr1 KO小鼠中降低Tau。不同基因型的雄性后代接受了各种与自闭症相关的行为测试、RNA测序和生化分析。Fmr1-KO雄性小鼠用Tau靶向反义寡核苷酸(ASO)处理,然后进行行为测试和生化分析。结果:Fmr1-KO小鼠皮层Tau表达增加。基因减少Tau可以预防Fmr1-KO小鼠的社会缺陷、定型和重复行为以及脊柱异常。Tau的减少还逆转了Fmr1-KO小鼠中增加的周期性活性,并部分挽救了Per1表达的减少。此外,Tau的减少逆转了Fmr1-KO小鼠中受损的P38/MAPK信号传导。最后,靶向ASO的Tau也有效地减轻了Fmr1-KO小鼠的自闭症样表型,并促进了P38/MAPK信号传导。局限性:我们的研究仅限于雄性小鼠,这与雄性小鼠FXS的发病率高于雌性小鼠一致。Tau的减少是否也对女性起到了保护作用,值得进一步研究。此外,尽管Tau减少挽救了Fmr1-KO小鼠中受损的P38/MAPK信号传导,但这是否是负责任的分子机制需要进一步确定。结论:我们的数据表明,Tau减少可以预防Fmr1-KO小鼠的自闭症样表型。Tau可能成为FXS治疗的新靶点。
{"title":"Tau reduction attenuates autism-like features in Fmr1 knockout mice.","authors":"Shanshan Zhao, Xiangyu Jiang, Linkun Han, Yiru Jiang, Yong Wang, Jian Meng, Xiang Zhu, Xian Zhang, Hong Luo, Yun-Wu Zhang","doi":"10.1186/s13229-023-00574-1","DOIUrl":"10.1186/s13229-023-00574-1","url":null,"abstract":"<p><strong>Background: </strong>Fragile X syndrome (FXS) is a leading cause of autism spectrum disorder (ASD) and resulted from a loss of the FMR1-encoded fragile X messenger ribonucleoprotein 1 (FMRP) protein due to large CGG repeat expansions in the promoter region of the FMR1 gene. The microtubule-associated protein Tau is a promising target for Tauopathic diseases and our preliminary study found that Tau protein levels were increased in the brain of Fmr1 knockout (KO) mice, a model of FXS. However, whether Tau reduction can prevent autism-like features in Fmr1 KO mice and become a novel strategy for FXS treatment remain unknown.</p><p><strong>Methods: </strong>Tau was genetically reduced in Fmr1 KO mice through crossing Fmr1<sup>±</sup> female mice with Mapt<sup>±</sup> male mice. The male offspring with different genotypes were subjected to various autism-related behavioral tests, RNA sequencing, and biochemical analysis. Fmr1 KO male mice were treated with Tau-targeting antisense oligonucleotide (ASO) and then subjected to behavioral tests and biochemical analysis.</p><p><strong>Results: </strong>Tau expression was increased in the cortex of Fmr1 KO mice. Genetically reducing Tau prevented social defects, stereotyped and repetitive behavior, and spine abnormality in Fmr1 KO mice. Tau reduction also reversed increased periodic activity and partially rescued Per1 expression reduction in Fmr1 KO mice. Moreover, Tau reduction reversed compromised P38/MAPK signaling in Fmr1 KO mice. Finally, Tau-targeting ASO also effectively alleviated autism-like phenotypes and promoted P38/MAPK signaling in Fmr1 KO mice.</p><p><strong>Limitations: </strong>Our study is limited to male mice, in agreement with the higher incidence of FXS in males than females. Whether Tau reduction also exerts protection in females deserves further scrutiny. Moreover, although Tau reduction rescues impaired P38/MAPK signaling in Fmr1 KO mice, whether this is the responsible molecular mechanism requires further determination.</p><p><strong>Conclusion: </strong>Our data indicate that Tau reduction prevents autism-like phenotypes in Fmr1 KO mice. Tau may become a new target for FXS treatment.</p>","PeriodicalId":18733,"journal":{"name":"Molecular Autism","volume":"14 1","pages":"42"},"PeriodicalIF":6.2,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developmental prediction modeling based on diffusion tensor imaging uncovering age-dependent heterogeneity in early childhood autistic brain. 基于扩散张量成像的发展预测模型揭示了儿童早期自闭症大脑中年龄依赖性的异质性。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-30 DOI: 10.1186/s13229-023-00573-2
Xinyue Huang, Yating Ming, Weixing Zhao, Rui Feng, Yuanyue Zhou, Lijie Wu, Jia Wang, Jinming Xiao, Lei Li, Xiaolong Shan, Jing Cao, Xiaodong Kang, Huafu Chen, Xujun Duan

Objective: There has been increasing evidence for atypical white matter (WM) microstructure in autistic people, but findings have been divergent. The development of autistic people in early childhood is clouded by the concurrently rapid brain growth, which might lead to the inconsistent findings of atypical WM microstructure in autism. Here, we aimed to reveal the developmental nature of autistic children and delineate atypical WM microstructure throughout early childhood while taking developmental considerations into account.

Method: In this study, diffusion tensor imaging was acquired from two independent cohorts, containing 91 autistic children and 100 typically developing children (TDC), aged 4-7 years. Developmental prediction modeling using support vector regression based on TDC participants was conducted to estimate the WM atypical development index of autistic children. Then, subgroups of autistic children were identified by using the k-means clustering method and were compared to each other on the basis of demographic information, WM atypical development index, and autistic trait by using two-sample t-test. Relationship of the WM atypical development index with age was estimated by using partial correlation. Furthermore, we performed threshold-free cluster enhancement-based two-sample t-test for the group comparison in WM microstructures of each subgroup of autistic children with the rematched subsets of TDC.

Results: We clustered autistic children into two subgroups according to WM atypical development index. The two subgroups exhibited distinct developmental stages and age-dependent diversity. WM atypical development index was found negatively associated with age. Moreover, an inverse pattern of atypical WM microstructures and different clinical manifestations in the two stages, with subgroup 1 showing overgrowth with low level of autistic traits and subgroup 2 exhibiting delayed maturation with high level of autistic traits, were revealed.

Conclusion: This study illustrated age-dependent heterogeneity in early childhood autistic children and delineated developmental stage-specific difference that ranged from an overgrowth pattern to a delayed pattern. Trial registration This study has been registered at ClinicalTrials.gov (Identifier: NCT02807766) on June 21, 2016 ( https://clinicaltrials.gov/ct2/show/NCT02807766 ).

目的:越来越多的证据表明自闭症患者存在非典型白质(WM)微观结构,但研究结果存在分歧。自闭症患者在儿童早期的发展受到同时快速大脑生长的影响,这可能导致自闭症中非典型WM微观结构的不一致发现。在这里,我们旨在揭示自闭症儿童的发展本质,并在考虑发展因素的同时,描绘整个幼儿期非典型WM微观结构。方法:在本研究中,从两个独立的队列中获得扩散张量成像,包括91名自闭症儿童和100名4-7岁的典型发育中儿童(TDC)。基于TDC参与者,使用支持向量回归进行发展预测建模,以估计自闭症儿童的WM非典型发展指数。然后,使用k-means聚类方法确定自闭症儿童的亚组,并使用双样本t检验在人口统计学信息、WM非典型发展指数和自闭症特征的基础上相互比较。WM非典型发展指数和年龄的关系用偏相关估计。此外,我们对自闭症儿童各亚组与TDC亚组的WM微观结构进行了基于无阈值聚类增强的双样本t检验。结果:我们根据WM非典型发育指数将自闭症儿童分为两个亚组。这两个亚组表现出不同的发育阶段和年龄依赖性的多样性。WM非典型发育指数与年龄呈负相关。此外,在这两个阶段中,非典型WM微观结构和不同临床表现的相反模式被揭示,亚组1表现出过度生长,具有低水平的自闭症特征,而亚组2表现出延迟成熟,具有高水平的自闭主义特征。结论:本研究阐明了儿童早期自闭症儿童的年龄依赖性异质性,并描绘了从过度生长模式到延迟模式的发育阶段特异性差异。试验注册本研究已于2016年6月21日在ClinicalTrials.gov(标识符:NCT02807766)上注册(https://clinicaltrials.gov/ct2/show/NCT02807766)。
{"title":"Developmental prediction modeling based on diffusion tensor imaging uncovering age-dependent heterogeneity in early childhood autistic brain.","authors":"Xinyue Huang,&nbsp;Yating Ming,&nbsp;Weixing Zhao,&nbsp;Rui Feng,&nbsp;Yuanyue Zhou,&nbsp;Lijie Wu,&nbsp;Jia Wang,&nbsp;Jinming Xiao,&nbsp;Lei Li,&nbsp;Xiaolong Shan,&nbsp;Jing Cao,&nbsp;Xiaodong Kang,&nbsp;Huafu Chen,&nbsp;Xujun Duan","doi":"10.1186/s13229-023-00573-2","DOIUrl":"10.1186/s13229-023-00573-2","url":null,"abstract":"<p><strong>Objective: </strong>There has been increasing evidence for atypical white matter (WM) microstructure in autistic people, but findings have been divergent. The development of autistic people in early childhood is clouded by the concurrently rapid brain growth, which might lead to the inconsistent findings of atypical WM microstructure in autism. Here, we aimed to reveal the developmental nature of autistic children and delineate atypical WM microstructure throughout early childhood while taking developmental considerations into account.</p><p><strong>Method: </strong>In this study, diffusion tensor imaging was acquired from two independent cohorts, containing 91 autistic children and 100 typically developing children (TDC), aged 4-7 years. Developmental prediction modeling using support vector regression based on TDC participants was conducted to estimate the WM atypical development index of autistic children. Then, subgroups of autistic children were identified by using the k-means clustering method and were compared to each other on the basis of demographic information, WM atypical development index, and autistic trait by using two-sample t-test. Relationship of the WM atypical development index with age was estimated by using partial correlation. Furthermore, we performed threshold-free cluster enhancement-based two-sample t-test for the group comparison in WM microstructures of each subgroup of autistic children with the rematched subsets of TDC.</p><p><strong>Results: </strong>We clustered autistic children into two subgroups according to WM atypical development index. The two subgroups exhibited distinct developmental stages and age-dependent diversity. WM atypical development index was found negatively associated with age. Moreover, an inverse pattern of atypical WM microstructures and different clinical manifestations in the two stages, with subgroup 1 showing overgrowth with low level of autistic traits and subgroup 2 exhibiting delayed maturation with high level of autistic traits, were revealed.</p><p><strong>Conclusion: </strong>This study illustrated age-dependent heterogeneity in early childhood autistic children and delineated developmental stage-specific difference that ranged from an overgrowth pattern to a delayed pattern. Trial registration This study has been registered at ClinicalTrials.gov (Identifier: NCT02807766) on June 21, 2016 ( https://clinicaltrials.gov/ct2/show/NCT02807766 ).</p>","PeriodicalId":18733,"journal":{"name":"Molecular Autism","volume":"14 1","pages":"41"},"PeriodicalIF":6.2,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71413090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of presentation rate on auditory processing in Rett syndrome: event-related potential study. Rett综合征呈现率对听觉处理的影响:事件相关电位研究。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-26 DOI: 10.1186/s13229-023-00566-1
Daria Kostanian, Anna Rebreikina, Victoria Voinova, Olga Sysoeva

Background: Rett syndrome (RS) is a rare neurodevelopmental disorder characterized by mutations in the MECP2 gene. Patients with RS have severe motor abnormalities and are often unable to walk, use hands and speak. The preservation of perceptual and cognitive functions is hard to assess, while clinicians and care-givers point out that these patients need more time to process information than typically developing peers. Neurophysiological correlates of auditory processing have been also found to be distorted in RS, but sound presentation rates were relatively quick in these studies (stimulus onset asynchrony, SOA < 1000 ms). As auditory event-related potential (ERP) is typically increased with prolongation of SOA we aim to study if SOA prolongation might compensate for observed abnormalities.

Methods: We presented a repetitive stimulus (1000 Hz) at three different SOAs of 900 ms, 1800 ms, and 3600 ms in children with RS (N = 24, Mean age = 9.0 ± 3.1) and their typical development (TD) peers (N = 27, Mean age = 9.7 ± 3.4) while recording 28-channels electroencephalogram, EEG. Some RS participants (n = 10) did not show clear ERP and were excluded from the analysis.

Results: Major ERP components (here assessed as N1P1 and P2N1 peak-to-peak values) were smaller at SOA 900 than at longer SOAs in both groups, pointing out that the basic mechanism of adaptation in the auditory system is preserved in at least in RS patients with evident ERPs. At the same time the latencies of these components were significantly delayed in the RS than in TD. Moreover, late components (P2N1 and N2P2) were drastically reduced in Rett syndrome irrespective of the SOA, suggesting a largely affected mechanism of integration of upcoming sensory input with memory. Moreover, developmental stagnation of auditory ERP characterized patients with RS: absence of typical P2N1 enlargement and P1 and N1 shortening with age at least for shortest SOA.

Limitations: We could not figure out the cause for the high percentage of no-evident ERP RS participants and our final sample of the RS group was rather small. Also, our study did not include a control clinical group.

Conclusions: Thus, auditory ERPs inform us about abnormalities within auditory processing that cannot be fully overcomed by slowing presentation rate.

背景:雷特综合征是一种罕见的以MECP2基因突变为特征的神经发育障碍。RS患者有严重的运动异常,通常无法行走、用手和说话。感知和认知功能的保存很难评估,而临床医生和护理人员指出,这些患者比通常发育中的同龄人需要更多的时间来处理信息。在RS中,听觉处理的神经生理学相关性也被发现是扭曲的,但在这些研究中,声音呈现率相对较快(刺激发作不同步,SOA 方法:我们在患有RS(N = 24,平均年龄 = 9 ± 3.1)及其典型发展(TD)同行(N = 27,平均年龄 = 9.7 ± 3.4)同时记录28个通道的脑电图。一些RS参与者(n = 10) 没有显示出明确的ERP,因此被排除在分析之外。结果:在SOA 900时,两组的主要ERP成分(此处评估为N1P1和P2N1峰间值)均小于较长SOA时,这表明至少在具有明显ERP的RS患者中,听觉系统的基本适应机制得以保留。同时,与TD相比,RS中这些成分的潜伏期显著延迟。此外,无论SOA如何,Rett综合征中的晚期成分(P2N1和N2P2)都显著减少,这表明即将到来的感觉输入与记忆的整合机制受到了很大影响。此外,听觉ERP的发育停滞是RS患者的特征:至少在最短的SOA中,没有典型的P2N1增大和P1和N1随年龄缩短。局限性:我们无法找出没有明显ERP RS参与者的高百分比的原因,我们对RS组的最终样本相当小。此外,我们的研究不包括对照临床组。结论:因此,听觉ERPs告知我们听觉处理中的异常,这些异常不能通过减慢呈现率来完全克服。
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引用次数: 1
Translatome analysis of tuberous sclerosis complex 1 patient-derived neural progenitor cells reveals rapamycin-dependent and independent alterations. 结节性硬化综合征1患者来源的神经祖细胞的Translatomy分析显示雷帕霉素依赖性和独立性改变。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-25 DOI: 10.1186/s13229-023-00572-3
Inci S Aksoylu, Pauline Martin, Francis Robert, Krzysztof J Szkop, Nicholas E Redmond, Srirupa Bhattacharyya, Jennifer Wang, Shan Chen, Roberta L Beauchamp, Irene Nobeli, Jerry Pelletier, Ola Larsson, Vijaya Ramesh

Background: Tuberous sclerosis complex (TSC) is an inherited neurocutaneous disorder caused by mutations in the TSC1 or TSC2 genes, with patients often exhibiting neurodevelopmental (ND) manifestations termed TSC-associated neuropsychiatric disorders (TAND) including autism spectrum disorder (ASD) and intellectual disability. Hamartin (TSC1) and tuberin (TSC2) proteins form a complex inhibiting mechanistic target of rapamycin complex 1 (mTORC1) signaling. Loss of TSC1 or TSC2 activates mTORC1 that, among several targets, controls protein synthesis by inhibiting translational repressor eIF4E-binding proteins. Using TSC1 patient-derived neural progenitor cells (NPCs), we recently reported early ND phenotypic changes, including increased cell proliferation and altered neurite outgrowth in TSC1-null NPCs, which were unaffected by the mTORC1 inhibitor rapamycin.

Methods: Here, we used polysome profiling, which quantifies changes in mRNA abundance and translational efficiencies at a transcriptome-wide level, to compare CRISPR-edited TSC1-null with CRISPR-corrected TSC1-WT NPCs generated from one TSC donor (one clone/genotype). To assess the relevance of identified gene expression alterations, we performed polysome profiling in postmortem brains from ASD donors and age-matched controls. We further compared effects on translation of a subset of transcripts and rescue of early ND phenotypes in NPCs following inhibition of mTORC1 using the allosteric inhibitor rapamycin versus a third-generation bi-steric, mTORC1-selective inhibitor RMC-6272.

Results: Polysome profiling of NPCs revealed numerous TSC1-associated alterations in mRNA translation that were largely recapitulated in human ASD brains. Moreover, although rapamycin treatment partially reversed the TSC1-associated alterations in mRNA translation, most genes related to neural activity/synaptic regulation or ASD were rapamycin-insensitive. In contrast, treatment with RMC-6272 inhibited rapamycin-insensitive translation and reversed TSC1-associated early ND phenotypes including proliferation and neurite outgrowth that were unaffected by rapamycin.

Conclusions: Our work reveals ample mRNA translation alterations in TSC1 patient-derived NPCs that recapitulate mRNA translation in ASD brain samples. Further, suppression of TSC1-associated but rapamycin-insensitive translation and ND phenotypes by RMC-6272 unveils potential implications for more efficient targeting of mTORC1 as a superior treatment strategy for TAND.

背景:结节性硬化综合征(TSC)是一种由TSC1或TSC2基因突变引起的遗传性神经皮肤疾病,患者经常表现出神经发育(ND)表现,称为TSC相关神经精神障碍(TAND),包括自闭症谱系障碍(ASD)和智力残疾。Hamartin(TSC1)和tuberin(TSC2)蛋白形成雷帕霉素复合物1(mTORC1)信号传导的复合物抑制机制靶标。TSC1或TSC2的缺失激活mTORC1,在几个靶标中,mTORC1通过抑制翻译阻遏物eIF4E结合蛋白来控制蛋白质合成。使用TSC1患者来源的神经祖细胞(NPC),我们最近报道了早期ND表型变化,包括TSC1缺失的NPC中细胞增殖增加和轴突生长改变,这些细胞不受mTORC1抑制剂雷帕霉素的影响。方法:在这里,我们使用多组分析,在转录组水平上量化mRNA丰度和翻译效率的变化,来比较CRISPR编辑的TSC1-null和由一个TSC供体(一个克隆/基因型)产生的CRISPR校正的TSC1-WT NPC。为了评估已确定的基因表达改变的相关性,我们对ASD供体和年龄匹配的对照组的死后大脑进行了多聚体分析。我们进一步比较了在使用变构抑制剂雷帕霉素抑制mTORC1后对NPCs中转录物子集的翻译和早期ND表型的拯救的影响与第三代双空间,mTORC1选择性抑制剂RMC-6272结果:NPC的多聚体图谱显示了许多与TSC1相关的mRNA翻译变化,这些变化在人类ASD大脑中大量重现。此外,尽管雷帕霉素治疗部分逆转了TSC1相关的mRNA翻译改变,但大多数与神经活动/突触调节或ASD相关的基因对雷帕霉素不敏感。相反,RMC-6272治疗抑制了雷帕霉素不敏感的翻译,并逆转了TSC1相关的早期ND表型,包括不受雷帕霉素影响的增殖和轴突生长。结论:我们的工作揭示了TSC1患者来源的NPC中大量的mRNA翻译改变,这些改变概括了ASD脑样本中的mRNA翻译。此外,RMC-6272对TSC1相关但雷帕霉素不敏感的翻译和ND表型的抑制揭示了更有效地靶向mTORC1作为TAND的优越治疗策略的潜在意义。
{"title":"Translatome analysis of tuberous sclerosis complex 1 patient-derived neural progenitor cells reveals rapamycin-dependent and independent alterations.","authors":"Inci S Aksoylu, Pauline Martin, Francis Robert, Krzysztof J Szkop, Nicholas E Redmond, Srirupa Bhattacharyya, Jennifer Wang, Shan Chen, Roberta L Beauchamp, Irene Nobeli, Jerry Pelletier, Ola Larsson, Vijaya Ramesh","doi":"10.1186/s13229-023-00572-3","DOIUrl":"10.1186/s13229-023-00572-3","url":null,"abstract":"<p><strong>Background: </strong>Tuberous sclerosis complex (TSC) is an inherited neurocutaneous disorder caused by mutations in the TSC1 or TSC2 genes, with patients often exhibiting neurodevelopmental (ND) manifestations termed TSC-associated neuropsychiatric disorders (TAND) including autism spectrum disorder (ASD) and intellectual disability. Hamartin (TSC1) and tuberin (TSC2) proteins form a complex inhibiting mechanistic target of rapamycin complex 1 (mTORC1) signaling. Loss of TSC1 or TSC2 activates mTORC1 that, among several targets, controls protein synthesis by inhibiting translational repressor eIF4E-binding proteins. Using TSC1 patient-derived neural progenitor cells (NPCs), we recently reported early ND phenotypic changes, including increased cell proliferation and altered neurite outgrowth in TSC1-null NPCs, which were unaffected by the mTORC1 inhibitor rapamycin.</p><p><strong>Methods: </strong>Here, we used polysome profiling, which quantifies changes in mRNA abundance and translational efficiencies at a transcriptome-wide level, to compare CRISPR-edited TSC1-null with CRISPR-corrected TSC1-WT NPCs generated from one TSC donor (one clone/genotype). To assess the relevance of identified gene expression alterations, we performed polysome profiling in postmortem brains from ASD donors and age-matched controls. We further compared effects on translation of a subset of transcripts and rescue of early ND phenotypes in NPCs following inhibition of mTORC1 using the allosteric inhibitor rapamycin versus a third-generation bi-steric, mTORC1-selective inhibitor RMC-6272.</p><p><strong>Results: </strong>Polysome profiling of NPCs revealed numerous TSC1-associated alterations in mRNA translation that were largely recapitulated in human ASD brains. Moreover, although rapamycin treatment partially reversed the TSC1-associated alterations in mRNA translation, most genes related to neural activity/synaptic regulation or ASD were rapamycin-insensitive. In contrast, treatment with RMC-6272 inhibited rapamycin-insensitive translation and reversed TSC1-associated early ND phenotypes including proliferation and neurite outgrowth that were unaffected by rapamycin.</p><p><strong>Conclusions: </strong>Our work reveals ample mRNA translation alterations in TSC1 patient-derived NPCs that recapitulate mRNA translation in ASD brain samples. Further, suppression of TSC1-associated but rapamycin-insensitive translation and ND phenotypes by RMC-6272 unveils potential implications for more efficient targeting of mTORC1 as a superior treatment strategy for TAND.</p>","PeriodicalId":18733,"journal":{"name":"Molecular Autism","volume":"14 1","pages":"39"},"PeriodicalIF":6.2,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50162121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-related changes in neural responses to sensory stimulation in autism: a cross-sectional study. 自闭症患者对感觉刺激的神经反应的年龄相关变化:一项横断面研究。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-11 DOI: 10.1186/s13229-023-00571-4
Melis E Cakar, Kaitlin K Cummings, Susan Y Bookheimer, Mirella Dapretto, Shulamite A Green

Background: Sensory over-responsivity (SOR) is an impairing sensory processing challenge in autism spectrum disorder (ASD) which shows heterogenous developmental trajectories and appears to improve into adulthood in some but not all autistic individuals. However, the neural mechanisms underlying interindividual differences in these trajectories are currently unknown.

Methods: Here, we used functional magnetic resonance imaging (fMRI) to investigate the association between age and neural activity linearly and nonlinearly in response to mildly aversive sensory stimulation as well as how SOR severity moderates this association. Participants included 52 ASD (14F) and 41 (13F) typically developing (TD) youth, aged 8.6-18.0 years.

Results: We found that in pre-teens, ASD children showed widespread activation differences in sensorimotor, frontal and cerebellar regions compared to TD children, while there were fewer differences between ASD and TD teens. In TD youth, older age was associated with less activation in the prefrontal cortex. In contrast, in ASD youth, older age was associated with more engagement of sensory integration and emotion regulation regions. In particular, orbitofrontal and medial prefrontal cortices showed a nonlinear relationship with age in ASD, with an especially steep increase in sensory-evoked neural activity during the mid-to-late teen years. There was also an interaction between age and SOR severity in ASD youth such that these age-related trends were more apparent in youth with higher SOR.

Limitations: The cross-sectional design limits causal interpretations of the data. Future longitudinal studies will be instrumental in determining how prefrontal engagement and SOR co-develop across adolescence.

Conclusions: Our results suggest that enhanced recruitment of prefrontal regions may underlie age-related decreases in SOR for a subgroup of ASD youth.

背景:在自闭症谱系障碍(ASD)中,感觉过度反应(SOR)是一种削弱感觉处理的挑战,表现出异质性的发展轨迹,在一些但并非所有自闭症个体中,似乎在成年后有所改善。然而,这些轨迹中个体间差异的神经机制目前尚不清楚。方法:在这里,我们使用功能性磁共振成像(fMRI)来研究年龄与对轻度厌恶性感觉刺激反应的神经活动之间的线性和非线性关系,以及SOR严重程度如何调节这种关系。参与者包括52名ASD(14F)和41名(13F)典型发育(TD)青年,年龄为8.6-18.0岁。结果:我们发现,与TD儿童相比,在青少年前期,ASD儿童在感觉运动、额叶和小脑区域表现出广泛的激活差异,而ASD和TD青少年之间的差异较小。在TD青年中,年龄越大,前额叶皮层的激活越少。相反,在ASD青年中,年龄越大,感觉统合和情绪调节区域的参与程度越高。特别是,ASD患者的眶额皮质和内侧前额叶皮质与年龄呈非线性关系,在青少年中后期,感觉诱发神经活动急剧增加。ASD青年的年龄和SOR严重程度之间也存在相互作用,因此这些与年龄相关的趋势在SOR较高的青年中更为明显。局限性:横断面设计限制了对数据的因果解释。未来的纵向研究将有助于确定前额叶参与和SOR如何在整个青春期共同发展。结论:我们的研究结果表明,前额叶区域的募集增强可能是ASD青年亚组SOR与年龄相关降低的原因。
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引用次数: 0
EEG functional connectivity in infants at elevated familial likelihood for autism spectrum disorder. 自闭症谱系障碍家族性可能性增高的婴儿脑电图功能连接。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-07 DOI: 10.1186/s13229-023-00570-5
Christian O'Reilly, Scott Huberty, Stefon van Noordt, James Desjardins, Nicky Wright, Julie Scorah, Sara Jane Webb, Mayada Elsabbagh

Background: Many studies have reported that autism spectrum disorder (ASD) is associated with atypical structural and functional connectivity. However, we know relatively little about the development of these differences in infancy.

Methods: We used a high-density electroencephalogram (EEG) dataset pooled from two independent infant sibling cohorts, to characterize such neurodevelopmental deviations during the first years of life. EEG was recorded at 6 and 12 months of age in infants at typical (N = 92) or elevated likelihood for ASD (N = 90), determined by the presence of an older sibling with ASD. We computed the functional connectivity between cortical sources of EEG during video watching using the corrected imaginary part of phase-locking values.

Results: Our main analysis found no significant association between functional connectivity and ASD, showing only significant effects for age, sex, age-sex interaction, and site. Given these null results, we performed an exploratory analysis and observed, at 12 months, a negative correlation between functional connectivity and ADOS calibrated severity scores for restrictive and repetitive behaviors (RRB).

Limitations: The small sample of ASD participants inherent to sibling studies limits diagnostic group comparisons. Also, results from our secondary exploratory analysis should be considered only as potential relationships to further explore, given their increased vulnerability to false positives.

Conclusions: These results are inconclusive concerning an association between EEG functional connectivity and ASD in infancy. Exploratory analyses provided preliminary support for a relationship between RRB and functional connectivity specifically, but these preliminary observations need corroboration on larger samples.

背景:许多研究报道,自闭症谱系障碍(ASD)与非典型的结构和功能连接有关。然而,我们对婴儿期这些差异的发展了解相对较少。方法:我们使用来自两个独立的婴儿兄弟姐妹队列的高密度脑电图(EEG)数据集来表征生命最初几年的神经发育偏差。在6个月和12个月大时,在典型(N = 92)或ASD可能性升高(N = 90),由患有ASD的年长兄弟姐妹的存在来确定。我们使用相位锁定值的校正虚部计算了视频观看过程中EEG皮层源之间的功能连接。结果:我们的主要分析发现,功能连接与ASD之间没有显著关联,仅对年龄、性别、年龄-性别互动和部位有显著影响。鉴于这些无效结果,我们进行了探索性分析,并在12个月时观察到,功能连接与ADOS校准的限制性和重复性行为严重程度评分(RRB)之间存在负相关。局限性:兄弟姐妹研究固有的ASD参与者的小样本限制了诊断组的比较。此外,鉴于二次探索性分析的结果更容易出现假阳性,因此只能将其视为需要进一步探索的潜在关系。结论:关于婴儿期脑电图功能连接与ASD之间的关系,这些结果是不确定的。探索性分析为RRB和功能连接之间的关系提供了初步支持,但这些初步观察结果需要在更大的样本上得到证实。
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引用次数: 0
The neuroanatomical substrates of autism and ADHD and their link to putative genomic underpinnings. 自闭症和多动症的神经解剖学基础及其与假定的基因组基础的联系。
IF 6.2 1区 医学 Q1 GENETICS & HEREDITY Pub Date : 2023-10-04 DOI: 10.1186/s13229-023-00568-z
Lisa M Berg, Caroline Gurr, Johanna Leyhausen, Hanna Seelemeyer, Anke Bletsch, Tim Schaefer, Charlotte M Pretzsch, Bethany Oakley, Eva Loth, Dorothea L Floris, Jan K Buitelaar, Christian F Beckmann, Tobias Banaschewski, Tony Charman, Emily J H Jones, Julian Tillmann, Chris H Chatham, Thomas Bourgeron, Declan G Murphy, Christine Ecker

Background: Autism spectrum disorders (ASD) are neurodevelopmental conditions accompanied by differences in brain development. Neuroanatomical differences in autism are variable across individuals and likely underpin distinct clinical phenotypes. To parse heterogeneity, it is essential to establish how the neurobiology of ASD is modulated by differences associated with co-occurring conditions, such as attention-deficit/hyperactivity disorder (ADHD). This study aimed to (1) investigate between-group differences in autistic individuals with and without co-occurring ADHD, and to (2) link these variances to putative genomic underpinnings.

Methods: We examined differences in cortical thickness (CT) and surface area (SA) and their genomic associations in a sample of 533 individuals from the Longitudinal European Autism Project. Using a general linear model including main effects of autism and ADHD, and an ASD-by-ADHD interaction, we examined to which degree ADHD modulates the autism-related neuroanatomy. Further, leveraging the spatial gene expression data of the Allen Human Brain Atlas, we identified genes whose spatial expression patterns resemble our neuroimaging findings.

Results: In addition to significant main effects for ASD and ADHD in fronto-temporal, limbic, and occipital regions, we observed a significant ASD-by-ADHD interaction in the left precentral gyrus and the right frontal gyrus for measures of CT and SA, respectively. Moreover, individuals with ASD + ADHD differed in CT to those without. Both main effects and the interaction were enriched for ASD-but not for ADHD-related genes.

Limitations: Although we employed a multicenter design to overcome single-site recruitment limitations, our sample size of N = 25 individuals in the ADHD only group is relatively small compared to the other subgroups, which limits the generalizability of the results. Also, we assigned subjects into ADHD positive groupings according to the DSM-5 rating scale. While this is sufficient for obtaining a research diagnosis of ADHD, our approach did not take into account for how long the symptoms have been present, which is typically considered when assessing ADHD in the clinical setting.

Conclusion: Thus, our findings suggest that the neuroanatomy of ASD is significantly modulated by ADHD, and that autistic individuals with co-occurring ADHD may have specific neuroanatomical underpinnings potentially mediated by atypical gene expression.

背景:自闭症谱系障碍(ASD)是伴随大脑发育差异的神经发育状况。自闭症的神经解剖学差异因个体而异,可能是不同临床表型的基础。为了分析异质性,有必要确定ASD的神经生物学是如何被与共同发生的条件相关的差异所调节的,例如注意力缺陷/多动障碍(ADHD)。本研究旨在(1)调查患有和不患有合并多动症的自闭症患者的组间差异,并(2)将这些差异与假定的基因组基础联系起来。方法:我们在来自欧洲自闭症纵向项目的533名个体样本中检测了皮层厚度(CT)和表面积(SA)的差异及其基因组关联。使用包括自闭症和多动症的主要影响以及由多动症相互作用引起的ASD的一般线性模型,我们研究了多动症在多大程度上调节自闭症相关的神经解剖学。此外,利用艾伦人脑图谱的空间基因表达数据,我们确定了空间表达模式与我们的神经影像学发现相似的基因。结果:除了对额颞区、边缘区和枕区的ASD和ADHD有显著的主要影响外,我们还分别在左中央前回和右额回观察到ASD与ADHD的显著相互作用。此外,ASD患者 + ADHD的CT表现与无CT者不同。ASD的主要作用和相互作用都很丰富,但ADHD相关基因却没有。局限性:尽管我们采用了多中心设计来克服单点招募的局限性,但我们的样本量N = 与其他亚组相比,仅患有多动症的组中的25人相对较小,这限制了结果的可推广性。此外,我们根据DSM-5评定量表将受试者分为多动症阳性组。虽然这足以获得多动症的研究诊断,但我们的方法没有考虑症状出现的时间,这通常是在临床环境中评估多动症时考虑的。结论:因此,我们的研究结果表明,自闭症谱系障碍的神经解剖学受到多动症的显著调节,同时患有多动症的自闭症患者可能具有特定的神经解剖学基础,可能由非典型基因表达介导。
{"title":"The neuroanatomical substrates of autism and ADHD and their link to putative genomic underpinnings.","authors":"Lisa M Berg, Caroline Gurr, Johanna Leyhausen, Hanna Seelemeyer, Anke Bletsch, Tim Schaefer, Charlotte M Pretzsch, Bethany Oakley, Eva Loth, Dorothea L Floris, Jan K Buitelaar, Christian F Beckmann, Tobias Banaschewski, Tony Charman, Emily J H Jones, Julian Tillmann, Chris H Chatham, Thomas Bourgeron, Declan G Murphy, Christine Ecker","doi":"10.1186/s13229-023-00568-z","DOIUrl":"10.1186/s13229-023-00568-z","url":null,"abstract":"<p><strong>Background: </strong>Autism spectrum disorders (ASD) are neurodevelopmental conditions accompanied by differences in brain development. Neuroanatomical differences in autism are variable across individuals and likely underpin distinct clinical phenotypes. To parse heterogeneity, it is essential to establish how the neurobiology of ASD is modulated by differences associated with co-occurring conditions, such as attention-deficit/hyperactivity disorder (ADHD). This study aimed to (1) investigate between-group differences in autistic individuals with and without co-occurring ADHD, and to (2) link these variances to putative genomic underpinnings.</p><p><strong>Methods: </strong>We examined differences in cortical thickness (CT) and surface area (SA) and their genomic associations in a sample of 533 individuals from the Longitudinal European Autism Project. Using a general linear model including main effects of autism and ADHD, and an ASD-by-ADHD interaction, we examined to which degree ADHD modulates the autism-related neuroanatomy. Further, leveraging the spatial gene expression data of the Allen Human Brain Atlas, we identified genes whose spatial expression patterns resemble our neuroimaging findings.</p><p><strong>Results: </strong>In addition to significant main effects for ASD and ADHD in fronto-temporal, limbic, and occipital regions, we observed a significant ASD-by-ADHD interaction in the left precentral gyrus and the right frontal gyrus for measures of CT and SA, respectively. Moreover, individuals with ASD + ADHD differed in CT to those without. Both main effects and the interaction were enriched for ASD-but not for ADHD-related genes.</p><p><strong>Limitations: </strong>Although we employed a multicenter design to overcome single-site recruitment limitations, our sample size of N = 25 individuals in the ADHD only group is relatively small compared to the other subgroups, which limits the generalizability of the results. Also, we assigned subjects into ADHD positive groupings according to the DSM-5 rating scale. While this is sufficient for obtaining a research diagnosis of ADHD, our approach did not take into account for how long the symptoms have been present, which is typically considered when assessing ADHD in the clinical setting.</p><p><strong>Conclusion: </strong>Thus, our findings suggest that the neuroanatomy of ASD is significantly modulated by ADHD, and that autistic individuals with co-occurring ADHD may have specific neuroanatomical underpinnings potentially mediated by atypical gene expression.</p>","PeriodicalId":18733,"journal":{"name":"Molecular Autism","volume":"14 1","pages":"36"},"PeriodicalIF":6.2,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41135918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Molecular Autism
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