Pub Date : 2023-10-01DOI: 10.1134/s0026893323050035
A. V. Burov, S. Yu. Funikov, T. M. Astakhova, E. V. Teterina, V. O. Nebogatikov, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov, A. V. Morozov
Abstract —Proteasomes are key components of the ubiquitin–proteasome system. Various forms of proteasomes are known. During aging, disturbances in the functioning of proteasomes have been revealed, as well as increased expression of their particular forms. Considering these data, we studied the expression of genes encoding the constitutive and immune subunits of proteasomes in cerebral cortex samples from C57BL/6 mice at the ages of 60, 190, 380, and 720 days. In addition, the contents of constitutive and immune proteasome subunits, chymotrypsin-like and caspase-like activities of proteasome pools, as well as the activity of the β5i immune subunit were studied in tissue homogenates. The chymotrypsin-like activity and the activity of the β5i subunit of different forms of proteasomes separated by electrophoresis in native gel were characterized. Compared with samples from young animals, in the cerebral cortex of animals at an age of 720 days the following changes in the expression patterns of proteasome genes were revealed: a decreased expression of the PSMB5 gene encoding constitutive proteasome subunit β5; increased expression of genes encoding immune proteasome subunits β5i and β1i. In tissue homogenates of aged mice, an increase in the content of immune subunits β1i and β2i was shown. In samples from old animals, chymotrypsin-like activity was decreased and a tendency to a decrease in caspase-like activity of proteasomes as well as the β5i subunit activity was revealed. Analysis of the activity of native complexes in tissues obtained from old animals revealed decreased chymotrypsin-like activity of 26S and 20S proteasomes containing the β5i subunit. Based on the obtained data, it can be assumed that changes in the pool of nonconstitutive proteasomes reflect aging-associated adaptive processes in the mouse brain.
{"title":"Dynamic Changes in the Activities and Contents of Particular Proteasome Forms in the Cerebral Cortex of C57BL/6 Mice during Aging","authors":"A. V. Burov, S. Yu. Funikov, T. M. Astakhova, E. V. Teterina, V. O. Nebogatikov, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov, A. V. Morozov","doi":"10.1134/s0026893323050035","DOIUrl":"https://doi.org/10.1134/s0026893323050035","url":null,"abstract":"Abstract —Proteasomes are key components of the ubiquitin–proteasome system. Various forms of proteasomes are known. During aging, disturbances in the functioning of proteasomes have been revealed, as well as increased expression of their particular forms. Considering these data, we studied the expression of genes encoding the constitutive and immune subunits of proteasomes in cerebral cortex samples from C57BL/6 mice at the ages of 60, 190, 380, and 720 days. In addition, the contents of constitutive and immune proteasome subunits, chymotrypsin-like and caspase-like activities of proteasome pools, as well as the activity of the β5i immune subunit were studied in tissue homogenates. The chymotrypsin-like activity and the activity of the β5i subunit of different forms of proteasomes separated by electrophoresis in native gel were characterized. Compared with samples from young animals, in the cerebral cortex of animals at an age of 720 days the following changes in the expression patterns of proteasome genes were revealed: a decreased expression of the PSMB5 gene encoding constitutive proteasome subunit β5; increased expression of genes encoding immune proteasome subunits β5i and β1i. In tissue homogenates of aged mice, an increase in the content of immune subunits β1i and β2i was shown. In samples from old animals, chymotrypsin-like activity was decreased and a tendency to a decrease in caspase-like activity of proteasomes as well as the β5i subunit activity was revealed. Analysis of the activity of native complexes in tissues obtained from old animals revealed decreased chymotrypsin-like activity of 26S and 20S proteasomes containing the β5i subunit. Based on the obtained data, it can be assumed that changes in the pool of nonconstitutive proteasomes reflect aging-associated adaptive processes in the mouse brain.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050138
A. V. Morozov, A. V. Burov, S. Yu. Funikov, E. V. Teterina, T. M. Astakhova, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov
{"title":"Changes in the Activities and Contents of Individual Forms of Proteasomes in Samples of the Cerebral Cortex during Pathology Development in 5xFAD Mice","authors":"A. V. Morozov, A. V. Burov, S. Yu. Funikov, E. V. Teterina, T. M. Astakhova, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov","doi":"10.1134/s0026893323050138","DOIUrl":"https://doi.org/10.1134/s0026893323050138","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136117531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050114
N. V. Maluchenko, A. N. Korovina, A. A. Saulina, V. M. Studitsky, A. V. Feofanov
{"title":"The Role of the WGR Domain in the Functions of PARP1 and PARP2","authors":"N. V. Maluchenko, A. N. Korovina, A. A. Saulina, V. M. Studitsky, A. V. Feofanov","doi":"10.1134/s0026893323050114","DOIUrl":"https://doi.org/10.1134/s0026893323050114","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050023
Y. V. Abalenikhina, A. V. Shchulkin, A. A. Seidkulieva, E. D. Rokunov, F. T. Gadzhieva, E. N. Yakusheva
{"title":"The Mechanism of Bimodal Effect of DL-Butyonine Sulfoximine on Constitutive Androstane and Pregnane X Receptors In Vitro","authors":"Y. V. Abalenikhina, A. V. Shchulkin, A. A. Seidkulieva, E. D. Rokunov, F. T. Gadzhieva, E. N. Yakusheva","doi":"10.1134/s0026893323050023","DOIUrl":"https://doi.org/10.1134/s0026893323050023","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135607181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050047
I. S. Kiselev, O. G. Kulakova, O. A. Baturina, M. R. Kabilov, A. N. Boyko, O. O. Favorova
{"title":"DNA Methylation Profile of CD14+ Monocytes Changes in Primary Progressive Multiple Sclerosis","authors":"I. S. Kiselev, O. G. Kulakova, O. A. Baturina, M. R. Kabilov, A. N. Boyko, O. O. Favorova","doi":"10.1134/s0026893323050047","DOIUrl":"https://doi.org/10.1134/s0026893323050047","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135607182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050151
A. A. Sheveleva, G. S. Krasnov, A. V. Kudryavtseva, A. V. Snezhkina, E. V. Bulavkina, S. N. Chirkov
{"title":"Analysis of the Complete Tomato Aspermy Virus Genomes Suggests Reassortment in Russian Isolates from Chrysanthemum","authors":"A. A. Sheveleva, G. S. Krasnov, A. V. Kudryavtseva, A. V. Snezhkina, E. V. Bulavkina, S. N. Chirkov","doi":"10.1134/s0026893323050151","DOIUrl":"https://doi.org/10.1134/s0026893323050151","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135607183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050059
K. V. Kopylova, Ed. W. Kasparov, I. V. Marchenko, M. V. Smolnikova
{"title":"Digital PCR as a Highly Sensitive Diagnostic Tool: A Review","authors":"K. V. Kopylova, Ed. W. Kasparov, I. V. Marchenko, M. V. Smolnikova","doi":"10.1134/s0026893323050059","DOIUrl":"https://doi.org/10.1134/s0026893323050059","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050060
L. A. Koval, E. N. Proshkina, N. V. Zemskaya, I. A. Solovev, E. V. Schegoleva, M. V. Shaposhnikov, A. A. Moskalev
{"title":"Drosophila melanogaster Lifespan Is Regulated by nejire Gene Expression in Peripheral Tissues and Nervous System","authors":"L. A. Koval, E. N. Proshkina, N. V. Zemskaya, I. A. Solovev, E. V. Schegoleva, M. V. Shaposhnikov, A. A. Moskalev","doi":"10.1134/s0026893323050060","DOIUrl":"https://doi.org/10.1134/s0026893323050060","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s0026893323050072
A. V. Kuznetsov, I. Yu. Grishin, D. N. Vtyurina
{"title":"Spatial Models of Piezo Proteins and Protein‒Protein Interaction Networks in Trichoplax Animals (Placozoa)","authors":"A. V. Kuznetsov, I. Yu. Grishin, D. N. Vtyurina","doi":"10.1134/s0026893323050072","DOIUrl":"https://doi.org/10.1134/s0026893323050072","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1134/s002689332305014x
A. I. Nadyrova, A. S. Kosnyrev, V. V. Ulyanova, E. V. Dudkina, V. I. Vershinina, O. N. Ilinskaya
{"title":"Efficiency of Escherichia coli and Bacillus subtilis Expression Systems for Production of Binase Mutants","authors":"A. I. Nadyrova, A. S. Kosnyrev, V. V. Ulyanova, E. V. Dudkina, V. I. Vershinina, O. N. Ilinskaya","doi":"10.1134/s002689332305014x","DOIUrl":"https://doi.org/10.1134/s002689332305014x","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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