Pub Date : 2023-12-07DOI: 10.1134/s0026893323060158
Yu. V. Shatalin, V. S. Shubina, M. E. Solovieva, V. S. Akatov
Abstract—Vitamin B12, or cobalamin, is essential for normal body function and is used in the therapies of different diseases. Vitamin B12 has anti-inflammatory and antioxidant properties that can play an important role in the prevention of some diseases. On the other hand, it has been reported that vitamin B12 in combination with such reducing agents as ascorbate (vitamin C) and thiols showed prooxidant activity. This review provides information on the roles of vitamin B12 in diseases accompanied by inflammation and oxidative stress and the effects of vitamin B12 administrated alone and in combinations with different reducing agents such as ascorbate and thiols on oxidative stress. In addition, the mechanisms of prooxidant actions of combinations of vitamin B12 with these reducing agents depending on the form of vitamin B12 (hydroxocobalamin and cyanocobalamin) are discussed. Understanding the mechanisms of prooxidant action of vitamin B12 is necessary for developing strategies for therapeutic administration of vitamin B12.
{"title":"The Redox-Catalytic Properties of Cobalamins","authors":"Yu. V. Shatalin, V. S. Shubina, M. E. Solovieva, V. S. Akatov","doi":"10.1134/s0026893323060158","DOIUrl":"https://doi.org/10.1134/s0026893323060158","url":null,"abstract":"<p><b>Abstract</b>—Vitamin B12, or cobalamin, is essential for normal body function and is used in the therapies of different diseases. Vitamin B12 has anti-inflammatory and antioxidant properties that can play an important role in the prevention of some diseases. On the other hand, it has been reported that vitamin B12 in combination with such reducing agents as ascorbate (vitamin C) and thiols showed prooxidant activity. This review provides information on the roles of vitamin B12 in diseases accompanied by inflammation and oxidative stress and the effects of vitamin B12 administrated alone and in combinations with different reducing agents such as ascorbate and thiols on oxidative stress. In addition, the mechanisms of prooxidant actions of combinations of vitamin B12 with these reducing agents depending on the form of vitamin B12 (hydroxocobalamin and cyanocobalamin) are discussed. Understanding the mechanisms of prooxidant action of vitamin B12 is necessary for developing strategies for therapeutic administration of vitamin B12.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.1134/s0026893323060134
E. Yu. Platonova, D. A. Golubev, N. V. Zemskaya, O. G. Shevchenko, S. A. Patov, M. V. Shaposhnikov, A. A. Moskalev
Abstract—Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (Sorbus aucuparia L.) on the lifespan and stress resistance of Drosophila melanogaster with the identification of possible mechanisms of its biological activity. It has been established that an ethanol extract of S. aucuparia berries, the main components of which are rutin and cyanidin-3-rutinoside, has a pronounced antioxidant activity in vitro. At the same time, treatment with rowan berry extract increased the resistance of D. melanogaster males to starvation, but reduced resistance to hyperthermia. In females, the extract reduced resistance to oxidative stress but increased resistance to hyperthermia. The effects of rowan berry extract on longevity depended both on its concentration and on the sex of fruit flies. In response to treatment with rowan berry extract, D. melanogaster males and females showed slight differences in the background level of expression of cellular stress response genes, including heat shock genes (hsp27, hsp68, and hsp83), oxidative stress resistance genes (hif1, nrf2, and sod1), circadian rhythm genes (clk and per), and the longevity gene sirt1, which may explain the differences in the observed effects.
{"title":"The Antioxidant and Geroprotective Properties of an Extract of Mountain Ash (Sorbus aucuparia L.) Fruits","authors":"E. Yu. Platonova, D. A. Golubev, N. V. Zemskaya, O. G. Shevchenko, S. A. Patov, M. V. Shaposhnikov, A. A. Moskalev","doi":"10.1134/s0026893323060134","DOIUrl":"https://doi.org/10.1134/s0026893323060134","url":null,"abstract":"<p><b>Abstract</b>—Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (<i>Sorbus aucuparia</i> L.) on the lifespan and stress resistance of <i>Drosophila melanogaster</i> with the identification of possible mechanisms of its biological activity. It has been established that an ethanol extract of <i>S. aucuparia</i> berries, the main components of which are rutin and cyanidin-3-rutinoside, has a pronounced antioxidant activity in vitro. At the same time, treatment with rowan berry extract increased the resistance of <i>D. melanogaster</i> males to starvation, but reduced resistance to hyperthermia. In females, the extract reduced resistance to oxidative stress but increased resistance to hyperthermia. The effects of rowan berry extract on longevity depended both on its concentration and on the sex of fruit flies. In response to treatment with rowan berry extract, <i>D. melanogaster</i> males and females showed slight differences in the background level of expression of cellular stress response genes, including heat shock genes (<i>hsp27</i>, <i>hsp68</i>, and <i>hsp83</i>), oxidative stress resistance genes (<i>hif1</i>, <i>nrf2</i>, and <i>sod1</i>), circadian rhythm genes (<i>clk</i> and <i>per</i>), and the longevity gene <i>sirt1</i>, which may explain the differences in the observed effects.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.1134/s0026893323060195
L. A. Varfolomeeva, E. A. Klimanova, S. V. Sidorenko, D. A. Fedorov, O. D. Lopina
Abstract
Melittin, a peptide from bee venom, was found to be able to interact with many proteins, including calmodulin target proteins and ion-transporting P-type ATPases. It is assumed that melittin mimics a protein module involved in protein-protein interactions within cells. Previously, a Na+/K+-ATPase containing the α1 isoform of the catalytic subunit was found to co-precipitate with a protein with a molecular weight of about 70 kDa that interacts with antibodies against melittin by cross immunoprecipitation. In the presence of a specific Na+/K+-ATPase inhibitor (ouabain), the amount of protein with a molecular weight of 70 kDa interacting with Na+/K+-ATPase increases. In order to identify melittin-like protein from murine kidney homogenate, a fraction of melittin-like proteins with a molecular weight of approximately 70 kDa was obtained using affinity chromatography with immobilized antibodies specific to melittin. By mass spectrometry analysis, the obtained protein fraction was found to contain three molecular chaperones of Hsp70 superfamily: mitochondrial mtHsp70 (mortalin), Hsp73, Grp78 (BiP) of endoplasmic reticulum. These data suggest that chaperones from the HSP-70 superfamily contain a melittin-like module.
{"title":"Identification of Melittin-Like Proteins with a Molecular Weight of 67 kDa that Interact with Na+/K+-ATPase","authors":"L. A. Varfolomeeva, E. A. Klimanova, S. V. Sidorenko, D. A. Fedorov, O. D. Lopina","doi":"10.1134/s0026893323060195","DOIUrl":"https://doi.org/10.1134/s0026893323060195","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Melittin, a peptide from bee venom, was found to be able to interact with many proteins, including calmodulin target proteins and ion-transporting P-type ATPases. It is assumed that melittin mimics a protein module involved in protein-protein interactions within cells. Previously, a Na<sup>+</sup>/K<sup>+</sup>-ATPase containing the α1 isoform of the catalytic subunit was found to co-precipitate with a protein with a molecular weight of about 70 kDa that interacts with antibodies against melittin by cross immunoprecipitation. In the presence of a specific Na<sup>+</sup>/K<sup>+</sup>-ATPase inhibitor (ouabain), the amount of protein with a molecular weight of 70 kDa interacting with Na<sup>+</sup>/K<sup>+</sup>-ATPase increases. In order to identify melittin-like protein from murine kidney homogenate, a fraction of melittin-like proteins with a molecular weight of approximately 70 kDa was obtained using affinity chromatography with immobilized antibodies specific to melittin. By mass spectrometry analysis, the obtained protein fraction was found to contain three molecular chaperones of Hsp70 superfamily: mitochondrial mtHsp70 (mortalin), Hsp73, Grp78 (BiP) of endoplasmic reticulum. These data suggest that chaperones from the HSP-70 superfamily contain a melittin-like module.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.1134/s0026893323060109
O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina
Abstract
δ-Aminolevulinic acid dehydratase (ALAD) is a key enzyme of the cytoplasmic heme biosynthesis pathway. The primary structure of the ALAD gene, the multimeric structure of the ALAD/hemB protein, and ALAD expression during the annual reproductive cycle were studied in the cold-water marine sponge Halisarca dujardinii. The results implicated the GATA-1 transcription factor and DNA methylation in regulating ALAD expression. Re-aggregation of sponge cells was accompanied by a decrease in ALAD expression and a change in the cell content of an active ALAD/hemB form. Further study of heme biosynthesis and the role of ALAD/hemB in morphogenesis of basal animals may provide new opportunities for treating pathologies in higher animals.
摘要δ-氨基乙酰脱水酶(ALAD)是细胞质血红素生物合成途径中的一个关键酶。研究了冷水海海绵Halisarca dujardinii中ALAD基因的一级结构、ALAD/hemB蛋白的多聚体结构以及ALAD在年生殖周期中的表达。研究结果表明,GATA-1 转录因子和 DNA 甲基化参与了 ALAD 表达的调控。海绵细胞的重新聚集伴随着 ALAD 表达的减少和活性 ALAD/hemB 形式细胞含量的变化。进一步研究血红素的生物合成以及 ALAD/hemB 在基础动物形态发生中的作用,可能会为治疗高等动物的病症提供新的机会。
{"title":"Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii","authors":"O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina","doi":"10.1134/s0026893323060109","DOIUrl":"https://doi.org/10.1134/s0026893323060109","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>δ-Aminolevulinic acid dehydratase (ALAD) is a key enzyme of the cytoplasmic heme biosynthesis pathway. The primary structure of the <i>ALAD</i> gene, the multimeric structure of the ALAD/hemB protein, and <i>ALAD</i> expression during the annual reproductive cycle were studied in the cold-water marine sponge <i>Halisarca dujardinii</i>. The results implicated the GATA-1 transcription factor and DNA methylation in regulating <i>ALAD</i> expression. Re-aggregation of sponge cells was accompanied by a decrease in <i>ALAD</i> expression and a change in the cell content of an active ALAD/hemB form. Further study of heme biosynthesis and the role of ALAD/hemB in morphogenesis of basal animals may provide new opportunities for treating pathologies in higher animals.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.1134/s0026893323060110
E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk
Abstract—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.
{"title":"The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin","authors":"E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk","doi":"10.1134/s0026893323060110","DOIUrl":"https://doi.org/10.1134/s0026893323060110","url":null,"abstract":"<p><b>Abstract</b>—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the <i>Tlr4</i> gene. Third, Tα1 significantly reduced p53 and the activity of the <i>P53</i> gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in <i>Ar-1</i> gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1134/S0026893323060043
D. A. Chernyavskij, I. I. Galkin, A. N. Pavlyuchenkova, A. Fedorov, M. Chelombitko
{"title":"Role of Mitochondria in Intestinal Epithelial Barrier Dysfunction in Inflammatory Bowel Disease","authors":"D. A. Chernyavskij, I. I. Galkin, A. N. Pavlyuchenkova, A. Fedorov, M. Chelombitko","doi":"10.1134/S0026893323060043","DOIUrl":"https://doi.org/10.1134/S0026893323060043","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138610738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1134/S002689332306002X
V. E. Baksheeva, Jr A. A. Zamyatnin, E. Y. Zernii
{"title":"Neuronal Calcium Sensor-1: A Zinc/Redox-Dependent Protein of Nervous System Signaling Pathways","authors":"V. E. Baksheeva, Jr A. A. Zamyatnin, E. Y. Zernii","doi":"10.1134/S002689332306002X","DOIUrl":"https://doi.org/10.1134/S002689332306002X","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138621362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1134/S0026893323060146
T. A. Seregina, I. Petrushanko, P. I. Zaripov, Yu. D. Kuleshova, K. V. Lobanov, R. Shakulov, V. Mitkevich, A. Makarov, A. Mironov
{"title":"Low-Molecular Thiols as a Factor Improving the Sensitivity of Escherichia coli Mutants with Impaired ADP–Heptose Synthesis to Antibiotics","authors":"T. A. Seregina, I. Petrushanko, P. I. Zaripov, Yu. D. Kuleshova, K. V. Lobanov, R. Shakulov, V. Mitkevich, A. Makarov, A. Mironov","doi":"10.1134/S0026893323060146","DOIUrl":"https://doi.org/10.1134/S0026893323060146","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138608295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1134/S0026893323060092
N. D. Kondratenko, L. A. Zinovkina, R. Zinovkin
{"title":"Transcription Factor NRF2 in Endothelial Functions","authors":"N. D. Kondratenko, L. A. Zinovkina, R. Zinovkin","doi":"10.1134/S0026893323060092","DOIUrl":"https://doi.org/10.1134/S0026893323060092","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138624169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1134/S0026893323060183
A. F. Vanin
{"title":"Dinityrosyl Iron Complexes with Thiol-Containing Ligands as a Functionally Active “Working Form” of Nitric Oxide System in Living Organisms: A Review","authors":"A. F. Vanin","doi":"10.1134/S0026893323060183","DOIUrl":"https://doi.org/10.1134/S0026893323060183","url":null,"abstract":"","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138623561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}