Pub Date : 2023-05-25DOI: 10.33245/2310-4902-2023-180-1-125-132
O. Shevchenko, D. Zasyekin
The article reflects the results of preclinical studies of the chronic toxicity of the disinfectant drug Iodopovidone on non-linear laboratory white rats. Disinfectant Iodopovidon produced by LLC "Basalt" (Ukraine) is a polymer complex of iodine in its composition. The purpose of our research was to establish the chronic toxicity of the new promising disinfectant drug Iodopovedon. The experiment was conducted on the basis of the laboratory and clinic "Vetmedservis" of the Faculty of Veterinary Medicine of the National University of Life and Environmental Sciences of Ukraine on non-linear laboratory white rats in compliance with biotic norms in accordance with the Law of Ukraine "On the Protection of Animals from Cruelty" and the "European Convention on the Protection of Domestic Animals". 10 animals were involved in the experiment (5 animals - experimental and 5 animals - control), groups were formed according to the principle of analogues. Biochemical indicators of blood serum (total protein, albumins, globulins, glucose, urea, creatinine and activity of enzymes AsLT, AsAT), hematological indicators of blood and organ mass ratio (liver, kidneys, heart) were determined. Determinations were made in accordance with generally accepted methods. As a result of the study of biochemical indicators, a difference in the levels of total protein between the experimental and control groups was established within 2.1% due to a decrease in the level of the globulin fraction by 0.3% and a decrease in the level of the albumin fraction by 3.7% and a tendency to lower glucose levels by 28.0%. There was also a trend of an increase in AST activity by 7.3% and a decrease in ALT activity by 3.3%. The level of creatinine did not undergo pronounced changes against the background of an increase in the level of urea by 11.7%. When examining hematological indicators, a slight decrease in the number of lymphocytes by 6.6% and an increase in monocytes and granulocytes by 5.5 and 13.6% were found, respectively. The established changes in biochemical indicators of blood serum and hematological indicators of blood are insignificant and unlikely. The results of studies of the relative mass of internal organs - liver, kidneys and heart, to the final body mass of animals, indicate the absence of deviations. Therefore, the disinfectant preparation Iodopovidon does not cause pronounced changes in the critically important organs of the liver, kidneys and heart, does not cause pronounced changes in the morphological parameters of the blood of animals, the biochemical composition and activity of enzymes in the blood serum of white laboratory rats. Key words: disinfectant, Iodopovidone, polymeric iodine complex, chronic toxicity, laboratory rats.
{"title":"Study of the chronic toxicity of the disinfectant drug Iodopovidone","authors":"O. Shevchenko, D. Zasyekin","doi":"10.33245/2310-4902-2023-180-1-125-132","DOIUrl":"https://doi.org/10.33245/2310-4902-2023-180-1-125-132","url":null,"abstract":"The article reflects the results of preclinical studies of the chronic toxicity of the disinfectant drug Iodopovidone on non-linear laboratory white rats. Disinfectant Iodopovidon produced by LLC \"Basalt\" (Ukraine) is a polymer complex of iodine in its composition. The purpose of our research was to establish the chronic toxicity of the new promising disinfectant drug Iodopovedon. The experiment was conducted on the basis of the laboratory and clinic \"Vetmedservis\" of the Faculty of Veterinary Medicine of the National University of Life and Environmental Sciences of Ukraine on non-linear laboratory white rats in compliance with biotic norms in accordance with the Law of Ukraine \"On the Protection of Animals from Cruelty\" and the \"European Convention on the Protection of Domestic Animals\". 10 animals were involved in the experiment (5 animals - experimental and 5 animals - control), groups were formed according to the principle of analogues. Biochemical indicators of blood serum (total protein, albumins, globulins, glucose, urea, creatinine and activity of enzymes AsLT, AsAT), hematological indicators of blood and organ mass ratio (liver, kidneys, heart) were determined. Determinations were made in accordance with generally accepted methods. As a result of the study of biochemical indicators, a difference in the levels of total protein between the experimental and control groups was established within 2.1% due to a decrease in the level of the globulin fraction by 0.3% and a decrease in the level of the albumin fraction by 3.7% and a tendency to lower glucose levels by 28.0%. There was also a trend of an increase in AST activity by 7.3% and a decrease in ALT activity by 3.3%. The level of creatinine did not undergo pronounced changes against the background of an increase in the level of urea by 11.7%. When examining hematological indicators, a slight decrease in the number of lymphocytes by 6.6% and an increase in monocytes and granulocytes by 5.5 and 13.6% were found, respectively. The established changes in biochemical indicators of blood serum and hematological indicators of blood are insignificant and unlikely. The results of studies of the relative mass of internal organs - liver, kidneys and heart, to the final body mass of animals, indicate the absence of deviations. Therefore, the disinfectant preparation Iodopovidon does not cause pronounced changes in the critically important organs of the liver, kidneys and heart, does not cause pronounced changes in the morphological parameters of the blood of animals, the biochemical composition and activity of enzymes in the blood serum of white laboratory rats. Key words: disinfectant, Iodopovidone, polymeric iodine complex, chronic toxicity, laboratory rats.","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136345982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.33245/2310-4902-2023-180-1-40-51
V. Lyasota, N. Bogatko, N. Bukalova, V. Dzmil, O. Hitska, T. Mazur, S. Tkachuk, T. Prilipko, B. Vakula
Against the background of the globalization of economic relations between countries, the activation of international trade is becoming urgent. In view of this, one of the main tasks for Ukraine as a member of the WTO and in connection with the prospect of its accession to the EU is the harmonization of national regulatory and legal requirements with international ones in the field of food safety and quality. The purpose of the research is to assess the safety and quality of honey of various types of individual domestic producers and to develop express, efficient methods for determining the quality of honey using the POCKET-PAL-3 refractometer and the safety of honey for establishing its adulteration with sodium hydrogen carbonate, alkaline detergents using indicators, as well as beet sugar by microscopy. The object of the study was the assessment of organoleptic, physico-chemical, microbiological parameters of natural bee honey of different botanical origins and varieties and detection of its adulteration, which was sold in supermarkets from various domestic manufacturers under the following trademarks. Analytical, organoleptic, physicochemical, microbiological, radiological, statistical methods of research were used to implement the research goal. Honey produced by different manufacturers according to organoleptic indicators: appearance, color, consistency, smell and taste, met the requirements of DSTU 4497:2005, except for sample №. 3, in which a discrepancy in taste was found. At the same time, according to physical and chemical parameters, honey samples №. 1, 2, 4 and 5 met the requirements of DSTU 4497:2005, except for sample №. 3 - by mass fraction of sucrose (to anhydrous substance) - 6.48±0.04 %, which did not meet the requirements of the current DSTU, the increase was 8.00 %. According to microbiological parameters and specific activity of Cs-137, it was established that all honey samples met the requirements of the national standard of Ukraine (DSTU 4497:2005). Tests were conducted to detect the adulteration of honey with sodium bicarbonate using the bromocresol green indicator, alkaline detergents using the chrome dark blue indicator, and beet sugar adulteration. For the express determination of the qualitative indicator - the mass fraction of honey, we used the POCKET-PAL-3 refractometer (Poland) for the first time. So, for the first time, we used the POCKET-PAL-3 refractometer (Poland) for the express determination of the mass fraction of water, which provides an opportunity to determine the mass fraction of dry matter with the subsequent calculation of the mass fraction of water, which ensures the reliability of the obtained indicators in 99.9 % compared to the indicators, established using a URL refractometer (model 1) in accordance with the requirements of DSTU 4497, and for the first time, patented methods of establishing the adulteration of honey with sodium bicarbonate, alkaline detergents were applied, the probability of the o
在国家间经济关系全球化的背景下，激活国际贸易已迫在眉睫。有鉴于此，乌克兰作为世贸组织成员的主要任务之一，以及与它加入欧盟的前景有关的主要任务之一，是使国家管理和法律要求与食品安全和质量领域的国际要求协调一致。本研究的目的是评估国内不同类型个体生产者生产的蜂蜜的安全性和质量，并开发快速、高效的方法，使用口袋- pal -3折光仪测定蜂蜜的质量，并通过显微镜测定蜂蜜的安全性，以确定其掺入碳酸氢钠、碱性洗涤剂和甜菜糖。本研究的目的是评估不同植物来源和品种的天然蜜蜂蜂蜜的感官、理化和微生物参数，并检测其掺假情况。采用分析学、感官学、理化、微生物学、放射学、统计学等方法进行研究。不同厂家根据感官指标生产的蜂蜜:外观，颜色，稠度，气味和味道，符合DSTU 4497:2005的要求，除了样品№。3，其中发现了味道的差异。同时，根据物理和化学参数，蜂蜜样品№。1、2、4和5符合DSTU 4497:2005的要求，除了样品№。3 -蔗糖(对无水物质)的质量分数- 6.48±0.04%，不符合现行DSTU的要求，增加了8.00 %。根据样品中Cs-137的微生物学参数和比活性测定，确定蜂蜜样品均符合乌克兰国家标准(DSTU 4497:2005)的要求。采用溴甲酚绿指示剂、铬深蓝色指示剂和甜菜糖掺假指示剂对蜂蜜进行了碳酸氢钠掺假检测。为了快速测定蜂蜜质量分数这一定性指标，我们首次使用波兰的POCKET-PAL-3折光计。因此，我们首次使用POCKET-PAL-3型折光仪(波兰)对水的质量分数进行了快速测定，为在计算水的质量分数的同时测定干物质的质量分数提供了机会，与DSTU 4497要求的URL折光仪(型号1)建立的指标相比，保证了所得指标的可靠性达到99.9%。采用专利方法建立了蜂蜜中碳酸氢钠的掺假，碱性洗涤剂，所得指标的概率为99.9%，以及用显微镜法掺假甜菜糖。关键词:天然蜂蜜，蜂蜜生产能力，感官，理化，微生物参数，安全，质量，消费者
{"title":"Safety and quality of natural bee honey produced under different trademarks as sold in supermarkets","authors":"V. Lyasota, N. Bogatko, N. Bukalova, V. Dzmil, O. Hitska, T. Mazur, S. Tkachuk, T. Prilipko, B. Vakula","doi":"10.33245/2310-4902-2023-180-1-40-51","DOIUrl":"https://doi.org/10.33245/2310-4902-2023-180-1-40-51","url":null,"abstract":"Against the background of the globalization of economic relations between countries, the activation of international trade is becoming urgent. In view of this, one of the main tasks for Ukraine as a member of the WTO and in connection with the prospect of its accession to the EU is the harmonization of national regulatory and legal requirements with international ones in the field of food safety and quality. The purpose of the research is to assess the safety and quality of honey of various types of individual domestic producers and to develop express, efficient methods for determining the quality of honey using the POCKET-PAL-3 refractometer and the safety of honey for establishing its adulteration with sodium hydrogen carbonate, alkaline detergents using indicators, as well as beet sugar by microscopy. The object of the study was the assessment of organoleptic, physico-chemical, microbiological parameters of natural bee honey of different botanical origins and varieties and detection of its adulteration, which was sold in supermarkets from various domestic manufacturers under the following trademarks. Analytical, organoleptic, physicochemical, microbiological, radiological, statistical methods of research were used to implement the research goal. Honey produced by different manufacturers according to organoleptic indicators: appearance, color, consistency, smell and taste, met the requirements of DSTU 4497:2005, except for sample №. 3, in which a discrepancy in taste was found. At the same time, according to physical and chemical parameters, honey samples №. 1, 2, 4 and 5 met the requirements of DSTU 4497:2005, except for sample №. 3 - by mass fraction of sucrose (to anhydrous substance) - 6.48±0.04 %, which did not meet the requirements of the current DSTU, the increase was 8.00 %. According to microbiological parameters and specific activity of Cs-137, it was established that all honey samples met the requirements of the national standard of Ukraine (DSTU 4497:2005). Tests were conducted to detect the adulteration of honey with sodium bicarbonate using the bromocresol green indicator, alkaline detergents using the chrome dark blue indicator, and beet sugar adulteration. For the express determination of the qualitative indicator - the mass fraction of honey, we used the POCKET-PAL-3 refractometer (Poland) for the first time. So, for the first time, we used the POCKET-PAL-3 refractometer (Poland) for the express determination of the mass fraction of water, which provides an opportunity to determine the mass fraction of dry matter with the subsequent calculation of the mass fraction of water, which ensures the reliability of the obtained indicators in 99.9 % compared to the indicators, established using a URL refractometer (model 1) in accordance with the requirements of DSTU 4497, and for the first time, patented methods of establishing the adulteration of honey with sodium bicarbonate, alkaline detergents were applied, the probability of the o","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136346088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.33245/2310-4902-2023-180-1-142-161
N. Ulianchych, S. Firstov, V. Kolomiiets, O. Koryak, L. Strilets, S. Rublenko, V. Chemerovskiy, T. Todosiuk, S. Shevchenko, M. Chornozub
According to clinical and radiological studies,the presented samples of calcium-phosphate ceramicimplants fully perform the osteoconductive function.However, in the case of their dense contact with theedges of the bone defect (experimental group), the primary inflammatory-resorptive stage of reparative osteogenesis turned out to be more intense and prolongedin time with pronounced phenomena of osteoresorptionof the mother bone and volumetric periosteal reactionas a compensatory mechanism. At the same time, osteoid formation is formed around the implant, while thecontrol implants are partially resorbed and replaced byosteoid tissue. As a result, macromorphologically, experimental implants in compact bone are visualized upto the 42nd day, and in cancellous bone - up to the 30thday, while the control implants are covered with boneregenerate already on the 14th day, which in the lattercase indicates the presence of osseointegration processes. The histological picture in the case of experimentalimplants confirms the prolongation of the inflammatory-resorptive stage with the formation of cartilage-boneregenerate around them, i.e. the dense placement of theimplants causes biomechanical pressure on the walls ofthe bone defect with the development of a reaction inthem, primarily to a foreign body.The study of the effect on reparative osteogenesis of the osseointegration properties of the developednew individual and standard-sized bioactive ceramicimplants based on clinical-radiological, macromorphological and histomorphological justification is relevant,as it will make it possible to evaluate their advantagesin terms of consolidation of complex defective fractures of tubular bones and reduce the frequency of theircomplications in animals. Key words: rabbits, bioceramics, reparative osteogenesis, bone defects.
{"title":"Comparison of osseointegration properties of individual and standard sized implants","authors":"N. Ulianchych, S. Firstov, V. Kolomiiets, O. Koryak, L. Strilets, S. Rublenko, V. Chemerovskiy, T. Todosiuk, S. Shevchenko, M. Chornozub","doi":"10.33245/2310-4902-2023-180-1-142-161","DOIUrl":"https://doi.org/10.33245/2310-4902-2023-180-1-142-161","url":null,"abstract":"According to clinical and radiological studies,the presented samples of calcium-phosphate ceramicimplants fully perform the osteoconductive function.However, in the case of their dense contact with theedges of the bone defect (experimental group), the primary inflammatory-resorptive stage of reparative osteogenesis turned out to be more intense and prolongedin time with pronounced phenomena of osteoresorptionof the mother bone and volumetric periosteal reactionas a compensatory mechanism. At the same time, osteoid formation is formed around the implant, while thecontrol implants are partially resorbed and replaced byosteoid tissue. As a result, macromorphologically, experimental implants in compact bone are visualized upto the 42nd day, and in cancellous bone - up to the 30thday, while the control implants are covered with boneregenerate already on the 14th day, which in the lattercase indicates the presence of osseointegration processes. The histological picture in the case of experimentalimplants confirms the prolongation of the inflammatory-resorptive stage with the formation of cartilage-boneregenerate around them, i.e. the dense placement of theimplants causes biomechanical pressure on the walls ofthe bone defect with the development of a reaction inthem, primarily to a foreign body.The study of the effect on reparative osteogenesis of the osseointegration properties of the developednew individual and standard-sized bioactive ceramicimplants based on clinical-radiological, macromorphological and histomorphological justification is relevant,as it will make it possible to evaluate their advantagesin terms of consolidation of complex defective fractures of tubular bones and reduce the frequency of theircomplications in animals. Key words: rabbits, bioceramics, reparative osteogenesis, bone defects.","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136346091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.33245/2310-4902-2023-180-1-81-88
M. Shevchenko, A. Andriichuk
Mastitis is the most common pathology of cows that causes large economic losses to dairy farms. Mastitis is often caused by a group of infectious associated pathogens that can be transmitted among animals. Most often, the pathological process in subclinically and clinically sick animals is caused by coccal gram-positive microflora. A major problem is the mechanisms by which microorganisms acquire resistance to one or more antibacterial agents. Thus, standard treatment regimens used on the farm become ineffective. The publication presents the results of the study of antibiotic resistance of 45 isolates of Staphylococcus spp. and 22 isolates of Streptococcus spp. In this study, the chromogenic media CHROMagarTM Mastitis, CHROMagarTM Orientation and CHROMagarTM MH Orientation were used, which helped to speed up the isolation and identification of cultures. Phenotypic antibiotic resistance profiles were determined using the agar diffusion method. Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) showed a high level of resistance to beta-lactams of the penicillin class of benzylpenicillin – 60% and 66.7%. Streptococcus disgalactiae and Streptococcus agalactiae showed high resistance to tetracycline – 46.7% and 35.3%. At the same time, Streptococcus agalactiae had a high resistance to clindamycin of 35.3%. Streptococcus disgalactiae to benzylpenicillin – 29.4%, Streptococus uberis to clindamycin – 75%. The lowest resistance was observed to the antibiotic vancomycin in 6.7% of isolated staphylococci and 13.3% of streptococci. MAR index of more than 0.2 was observed in 75% of Streptococus uberis, 60% of CoNS and 52.9% of Streptococcus agalactiae. More than 50% of all studied isolates had multiple resistance to antibiotics most commonly used on Ukrainian farms. Key words: Streptococcus spp., Staphylococcus spp., antibiotic resistance, mastitis, infectious mastitis, gram-positive bacteria.
{"title":"Antibiotic resistance of isolates of Staphylococcus spp. and Streptococcus spp. causing mastitis on dairy farms in Ukraine","authors":"M. Shevchenko, A. Andriichuk","doi":"10.33245/2310-4902-2023-180-1-81-88","DOIUrl":"https://doi.org/10.33245/2310-4902-2023-180-1-81-88","url":null,"abstract":"Mastitis is the most common pathology of cows that causes large economic losses to dairy farms. Mastitis is often caused by a group of infectious associated pathogens that can be transmitted among animals. Most often, the pathological process in subclinically and clinically sick animals is caused by coccal gram-positive microflora. A major problem is the mechanisms by which microorganisms acquire resistance to one or more antibacterial agents. Thus, standard treatment regimens used on the farm become ineffective. The publication presents the results of the study of antibiotic resistance of 45 isolates of Staphylococcus spp. and 22 isolates of Streptococcus spp. In this study, the chromogenic media CHROMagarTM Mastitis, CHROMagarTM Orientation and CHROMagarTM MH Orientation were used, which helped to speed up the isolation and identification of cultures. Phenotypic antibiotic resistance profiles were determined using the agar diffusion method. Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) showed a high level of resistance to beta-lactams of the penicillin class of benzylpenicillin – 60% and 66.7%. Streptococcus disgalactiae and Streptococcus agalactiae showed high resistance to tetracycline – 46.7% and 35.3%. At the same time, Streptococcus agalactiae had a high resistance to clindamycin of 35.3%. Streptococcus disgalactiae to benzylpenicillin – 29.4%, Streptococus uberis to clindamycin – 75%. The lowest resistance was observed to the antibiotic vancomycin in 6.7% of isolated staphylococci and 13.3% of streptococci. MAR index of more than 0.2 was observed in 75% of Streptococus uberis, 60% of CoNS and 52.9% of Streptococcus agalactiae. More than 50% of all studied isolates had multiple resistance to antibiotics most commonly used on Ukrainian farms. Key words: Streptococcus spp., Staphylococcus spp., antibiotic resistance, mastitis, infectious mastitis, gram-positive bacteria.","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136345976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.33245/2310-4902-2023-180-1-52-58
D. Ostrovskiy, V. Zotsenko, V. Grishko
Today, fusariotoxins play an increasing lyimportant role in the pathology of farm animals, among which deoxynivalenol plays an important role. Deoxynivalenol (DON) is one of the most common fusariotoxins. It affects the health of animals that consume contaminated feed, and can lead to various metabolic disorders, disrupting the body's homeostasis. One of the main effects of deoxynivalenol is that it stimulates the production of anti-inflammatory cytokines, which in turn causes the acute phase of inflammation in animals. In addition, deoxynivalenol leads to a decrease in appetite and can cause hypophagia in animals, which in turn leads to a decrease in daily gains. The direction of the conducted research is aimed at identifying the optimal substrate for the synthesis of deoxynivalenol and its maximum accumulation by the fungus F. graminearum, and also established the optimal parameters for the synthesis of mycotoxin (cultivation temperature, substrate humidity, cultivation period). Grains of the following crops were used as substrates: wheat, rice, corn, barley, oats, rye, millet, peas, soybeans, sunflower, mustard, rapeseed, buckwheat, and flax. Deoxynivalenol in samples was determined by thin-layer chromatography. The production of deoxynivalenol was studied in the temperature ranges of 4, 17, 24, and 28 °C; humidity of the substrate in the range from 14–90% and the duration of cultivation from 1 to 4 weeks. The mycotoxin-producing activity of the fungus F. graminearum isolate 195/1 was largely determined by the studied parameters. The maximum amount of deoxynivalenol was produced at a temperature of 24 ºС, a substrate humidity of 50%, and a duration of cultivation of 24 days. Of the tested substrates, rice was the best for deoxynivalenol production. The obtained results serve as a basis for possible prediction of feed contamination with deoxynivalenol and, in general, will allow to optimize measures to combat mycotoxicosis and thus minimize the possible risks of mycotoxin poisoning of people and animals. Key words: F. graminearum, grain substrates, deoxynivalenol, cultivation temperature, substrate humidity, duration of cultivation.
{"title":"Results of mycotoxicological studies of the influence of sanitary and hygienic conditions on the synthesis of deoxynivalenol by the micromycete F. graminearum on various grain substrates","authors":"D. Ostrovskiy, V. Zotsenko, V. Grishko","doi":"10.33245/2310-4902-2023-180-1-52-58","DOIUrl":"https://doi.org/10.33245/2310-4902-2023-180-1-52-58","url":null,"abstract":"Today, fusariotoxins play an increasing lyimportant role in the pathology of farm animals, among which deoxynivalenol plays an important role. Deoxynivalenol (DON) is one of the most common fusariotoxins. It affects the health of animals that consume contaminated feed, and can lead to various metabolic disorders, disrupting the body's homeostasis. One of the main effects of deoxynivalenol is that it stimulates the production of anti-inflammatory cytokines, which in turn causes the acute phase of inflammation in animals. In addition, deoxynivalenol leads to a decrease in appetite and can cause hypophagia in animals, which in turn leads to a decrease in daily gains. The direction of the conducted research is aimed at identifying the optimal substrate for the synthesis of deoxynivalenol and its maximum accumulation by the fungus F. graminearum, and also established the optimal parameters for the synthesis of mycotoxin (cultivation temperature, substrate humidity, cultivation period). Grains of the following crops were used as substrates: wheat, rice, corn, barley, oats, rye, millet, peas, soybeans, sunflower, mustard, rapeseed, buckwheat, and flax. Deoxynivalenol in samples was determined by thin-layer chromatography. The production of deoxynivalenol was studied in the temperature ranges of 4, 17, 24, and 28 °C; humidity of the substrate in the range from 14–90% and the duration of cultivation from 1 to 4 weeks. The mycotoxin-producing activity of the fungus F. graminearum isolate 195/1 was largely determined by the studied parameters. The maximum amount of deoxynivalenol was produced at a temperature of 24 ºС, a substrate humidity of 50%, and a duration of cultivation of 24 days. Of the tested substrates, rice was the best for deoxynivalenol production. The obtained results serve as a basis for possible prediction of feed contamination with deoxynivalenol and, in general, will allow to optimize measures to combat mycotoxicosis and thus minimize the possible risks of mycotoxin poisoning of people and animals. Key words: F. graminearum, grain substrates, deoxynivalenol, cultivation temperature, substrate humidity, duration of cultivation.","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136345983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32680/2409-9260-2023-7-8-308-309-118-125
Inna Kuznetsova, Vitaly Kuznetsov
{"title":"Management technology of site content on the basis of the process approach","authors":"Inna Kuznetsova, Vitaly Kuznetsov","doi":"10.32680/2409-9260-2023-7-8-308-309-118-125","DOIUrl":"https://doi.org/10.32680/2409-9260-2023-7-8-308-309-118-125","url":null,"abstract":"","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135709864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32680/2409-9260-2023-7-8-308-309-34-41
Tetiana Pohorielova, Alina Guzynska
{"title":"Economic development of Ukraine and its discriminant analysis","authors":"Tetiana Pohorielova, Alina Guzynska","doi":"10.32680/2409-9260-2023-7-8-308-309-34-41","DOIUrl":"https://doi.org/10.32680/2409-9260-2023-7-8-308-309-34-41","url":null,"abstract":"","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135712609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Functioning of ukrainian enterprises in the conditions of war: analysis of the situation and economic expectations","authors":"Tetiana Hudz, Svitlana Yehorycheva, Maksym Lytvyniuk","doi":"10.32680/2409-9260-2023-5-6-306-307-7-15","DOIUrl":"https://doi.org/10.32680/2409-9260-2023-5-6-306-307-7-15","url":null,"abstract":"","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136307726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32680/2409-9260-2023-5-6-306-307-123-128
Kateryna Onishchuk, Nataliia Bychkova
{"title":"Formation of the capital structure of world TNCs in the context of globalization changes","authors":"Kateryna Onishchuk, Nataliia Bychkova","doi":"10.32680/2409-9260-2023-5-6-306-307-123-128","DOIUrl":"https://doi.org/10.32680/2409-9260-2023-5-6-306-307-123-128","url":null,"abstract":"","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136306262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：