首页 > 最新文献

Nature Structural Biology最新文献

英文 中文
Current state of automated crystallographic data analysis. 自动晶体学数据分析的现状。
Pub Date : 2000-11-01 DOI: 10.1038/80763
V S Lamzin, A Perrakis

A goal of structural biology--and of structural genomics in particular--is to improve the underlying methodology for high-throughput determination of three-dimensional structures of biological macromolecules. Here we address issues related to the development, automation and streamlining of the process of macromolecular X-ray crystal structure solution.

结构生物学,特别是结构基因组学的一个目标是改进生物大分子三维结构的高通量测定的基本方法。在这里,我们讨论了与大分子x射线晶体结构溶液的开发、自动化和简化过程有关的问题。
{"title":"Current state of automated crystallographic data analysis.","authors":"V S Lamzin,&nbsp;A Perrakis","doi":"10.1038/80763","DOIUrl":"https://doi.org/10.1038/80763","url":null,"abstract":"<p><p>A goal of structural biology--and of structural genomics in particular--is to improve the underlying methodology for high-throughput determination of three-dimensional structures of biological macromolecules. Here we address issues related to the development, automation and streamlining of the process of macromolecular X-ray crystal structure solution.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 Suppl ","pages":"978-81"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/80763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 72
Target selection for structural genomics. 结构基因组学的靶标选择。
Pub Date : 2000-11-01 DOI: 10.1038/80747
S E Brenner

Structural genomics aims to use high-throughput structure determination and computational analysis to provide three-dimensional models of every tractable protein. The process of choosing proteins for experimental structure characterization is known as target selection. In this nomenclature, the targets are regions of proteins to be studied by crystallography or NMR. Selection of the targets is principally a computational process of restricting candidate proteins to those that are tractable and of unknown structure, and prioritizing according to expected interest and accessibility.

结构基因组学旨在利用高通量结构测定和计算分析来提供每个可处理蛋白质的三维模型。选择用于实验结构表征的蛋白质的过程称为靶标选择。在这个命名法中,目标是通过晶体学或核磁共振研究的蛋白质区域。靶标的选择主要是一个计算过程,将候选蛋白质限制在那些易于处理和结构未知的蛋白质中,并根据预期的兴趣和可及性进行优先排序。
{"title":"Target selection for structural genomics.","authors":"S E Brenner","doi":"10.1038/80747","DOIUrl":"https://doi.org/10.1038/80747","url":null,"abstract":"<p><p>Structural genomics aims to use high-throughput structure determination and computational analysis to provide three-dimensional models of every tractable protein. The process of choosing proteins for experimental structure characterization is known as target selection. In this nomenclature, the targets are regions of proteins to be studied by crystallography or NMR. Selection of the targets is principally a computational process of restricting candidate proteins to those that are tractable and of unknown structure, and prioritizing according to expected interest and accessibility.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 Suppl ","pages":"967-9"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/80747","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 119
Structural genomics projects in Japan. 日本的结构基因组学项目。
Pub Date : 2000-11-01 DOI: 10.1038/80712
S Yokoyama, H Hirota, T Kigawa, T Yabuki, M Shirouzu, T Terada, Y Ito, Y Matsuo, Y Kuroda, Y Nishimura, Y Kyogoku, K Miki, R Masui, S Kuramitsu

Two major structural genomics projects exist in Japan. The oldest, the RIKEN Structural Genomics Initiative, has two major goals: to determine bacterial, mammalian, and plant protein structures by X-ray crystallography and NMR spectroscopy and to perform functional analyses with the target proteins. The newest, the structural genomics project at the Biological Information Research Center, focuses on human membrane proteins.

日本有两个主要的结构基因组学项目。最早的是RIKEN结构基因组计划,有两个主要目标:通过x射线晶体学和核磁共振光谱确定细菌、哺乳动物和植物蛋白质结构,并对目标蛋白质进行功能分析。最新的是生物信息研究中心的结构基因组学项目,重点研究人类膜蛋白。
{"title":"Structural genomics projects in Japan.","authors":"S Yokoyama,&nbsp;H Hirota,&nbsp;T Kigawa,&nbsp;T Yabuki,&nbsp;M Shirouzu,&nbsp;T Terada,&nbsp;Y Ito,&nbsp;Y Matsuo,&nbsp;Y Kuroda,&nbsp;Y Nishimura,&nbsp;Y Kyogoku,&nbsp;K Miki,&nbsp;R Masui,&nbsp;S Kuramitsu","doi":"10.1038/80712","DOIUrl":"https://doi.org/10.1038/80712","url":null,"abstract":"<p><p>Two major structural genomics projects exist in Japan. The oldest, the RIKEN Structural Genomics Initiative, has two major goals: to determine bacterial, mammalian, and plant protein structures by X-ray crystallography and NMR spectroscopy and to perform functional analyses with the target proteins. The newest, the structural genomics project at the Biological Information Research Center, focuses on human membrane proteins.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 Suppl ","pages":"943-5"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/80712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 202
Patent protection for protein structures and databases. 蛋白质结构和数据库的专利保护。
Pub Date : 2000-11-01 DOI: 10.1038/80723
T C Meyers, T A Turano, D A Greenhalgh, P R Waller

Patent protection is available for certain inventions in the field of structural genomics. A review of the patent application procedure is provided, and patentable aspects of protein structural information under US law are discussed. Strategic and international factors to consider when seeking patent protection for an invention also are presented.

结构基因组学领域的某些发明可以获得专利保护。提供了专利申请程序的审查,并根据美国法律讨论了蛋白质结构信息的可专利性方面。在为一项发明寻求专利保护时要考虑的战略和国际因素也被提出。
{"title":"Patent protection for protein structures and databases.","authors":"T C Meyers,&nbsp;T A Turano,&nbsp;D A Greenhalgh,&nbsp;P R Waller","doi":"10.1038/80723","DOIUrl":"https://doi.org/10.1038/80723","url":null,"abstract":"<p><p>Patent protection is available for certain inventions in the field of structural genomics. A review of the patent application procedure is provided, and patentable aspects of protein structural information under US law are discussed. Strategic and international factors to consider when seeking patent protection for an invention also are presented.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 Suppl ","pages":"950-2"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/80723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Protein production: feeding the crystallographers and NMR spectroscopists. 蛋白质生产:供给晶体学家和核磁共振波谱学家。
Pub Date : 2000-11-01 DOI: 10.1038/80751
A M Edwards, C H Arrowsmith, D Christendat, A Dharamsi, J D Friesen, J F Greenblatt, M Vedadi

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

结构基因组学的蛋白质纯化工作将集中于容易表达的蛋白质的自动化,以及更难表达的蛋白质(如膜蛋白)的工艺开发。预计未来几年将生产出数千种蛋白质。纯化的蛋白质将成为整个研究界有价值的试剂。
{"title":"Protein production: feeding the crystallographers and NMR spectroscopists.","authors":"A M Edwards,&nbsp;C H Arrowsmith,&nbsp;D Christendat,&nbsp;A Dharamsi,&nbsp;J D Friesen,&nbsp;J F Greenblatt,&nbsp;M Vedadi","doi":"10.1038/80751","DOIUrl":"https://doi.org/10.1038/80751","url":null,"abstract":"<p><p>Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 Suppl ","pages":"970-2"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/80751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 158
Zinc ion mediated amino acid discrimination by threonyl-tRNA synthetase 锌离子介导的苏酰trna合成酶氨基酸识别
Pub Date : 2000-06-01 DOI: 10.1038/nsb0600_461
R. Sankaranarayanan, A. Dock‐Brégeon, B. Rees, M. Bovee, J. Caillet, P. Romby, C. Francklyn, D. Moras
{"title":"Zinc ion mediated amino acid discrimination by threonyl-tRNA synthetase","authors":"R. Sankaranarayanan, A. Dock‐Brégeon, B. Rees, M. Bovee, J. Caillet, P. Romby, C. Francklyn, D. Moras","doi":"10.1038/nsb0600_461","DOIUrl":"https://doi.org/10.1038/nsb0600_461","url":null,"abstract":"","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"35 1","pages":"461-465"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84554602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 131
Observing conformational and activity changes of tet repressor in vivo. 观察tet抑制因子在体内的构象和活性变化。
Pub Date : 2000-06-01 DOI: 10.1038/75883
B Tiebel, K Garke, W Hillen

Effector triggered conformational changes of proteins such as regulators of transcription, receptors, or enzymes are the molecular basis for regulation in biology. Most proteins perform their biological functions intracellularly, in the presence of many potential interaction partners. Studies of conformational changes have mainly been performed in vitro using sophisticated physical and biochemical methods that usually require purified proteins. Here we describe the observation of conformational changes of Tet repressor in the cytoplasm of growing Escherichia coli cells, analyzed by ligand dependent disulfide crosslinking of cysteine residues substituted into mobile regions of the protein. The amount of protein undergoing the structural change is quantitatively linked to the concomitant induction of transcription of a reporter gene.

效应器触发的蛋白质构象变化,如转录调节剂、受体或酶,是生物学调控的分子基础。大多数蛋白质在细胞内,在许多潜在的相互作用伙伴的存在下执行其生物学功能。构象变化的研究主要是在体外进行的,使用复杂的物理和生化方法,通常需要纯化的蛋白质。在这里,我们描述了在生长的大肠杆菌细胞细胞质中观察到的Tet抑制因子的构象变化,通过配体依赖的二硫交联分析了半胱氨酸残基取代到蛋白质的可移动区域。经历结构变化的蛋白质数量与报告基因转录的伴随诱导有定量联系。
{"title":"Observing conformational and activity changes of tet repressor in vivo.","authors":"B Tiebel,&nbsp;K Garke,&nbsp;W Hillen","doi":"10.1038/75883","DOIUrl":"https://doi.org/10.1038/75883","url":null,"abstract":"<p><p>Effector triggered conformational changes of proteins such as regulators of transcription, receptors, or enzymes are the molecular basis for regulation in biology. Most proteins perform their biological functions intracellularly, in the presence of many potential interaction partners. Studies of conformational changes have mainly been performed in vitro using sophisticated physical and biochemical methods that usually require purified proteins. Here we describe the observation of conformational changes of Tet repressor in the cytoplasm of growing Escherichia coli cells, analyzed by ligand dependent disulfide crosslinking of cysteine residues substituted into mobile regions of the protein. The amount of protein undergoing the structural change is quantitatively linked to the concomitant induction of transcription of a reporter gene.</p>","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"7 6","pages":"479-81"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/75883","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21723564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The SsrA–SmpB system for protein tagging, directed degradation and ribosome rescue SsrA-SmpB系统用于蛋白质标记,定向降解和核糖体救援
Pub Date : 2000-06-01 DOI: 10.1038/nsb0600_449
A. Karzai, E. Roche, R. Sauer
{"title":"The SsrA–SmpB system for protein tagging, directed degradation and ribosome rescue","authors":"A. Karzai, E. Roche, R. Sauer","doi":"10.1038/nsb0600_449","DOIUrl":"https://doi.org/10.1038/nsb0600_449","url":null,"abstract":"","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"10 1","pages":"449-455"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80867745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 403
Structure of Arf6–GDP suggests a basis for guanine nucleotide exchange factors specificity Arf6-GDP的结构提示了鸟嘌呤核苷酸交换因子特异性的基础
Pub Date : 2000-06-01 DOI: 10.1038/nsb0600_466
J. Ménétrey, E. Macia, S. Pasqualato, M. Franco, J. Cherfils
{"title":"Structure of Arf6–GDP suggests a basis for guanine nucleotide exchange factors specificity","authors":"J. Ménétrey, E. Macia, S. Pasqualato, M. Franco, J. Cherfils","doi":"10.1038/nsb0600_466","DOIUrl":"https://doi.org/10.1038/nsb0600_466","url":null,"abstract":"","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"90 1","pages":"466-469"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76202991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 58
Conformation of the myosin motor during force generation in skeletal muscle 骨骼肌力产生过程中肌球蛋白运动的构象
Pub Date : 2000-06-01 DOI: 10.1038/nsb0600_482
M. Irving, G. Piazzesi, L. Lucii, Yin-Biao Sun, J. Harford, I. Dobbie, M. Ferenczi, M. Reconditi, V. Lombardi
{"title":"Conformation of the myosin motor during force generation in skeletal muscle","authors":"M. Irving, G. Piazzesi, L. Lucii, Yin-Biao Sun, J. Harford, I. Dobbie, M. Ferenczi, M. Reconditi, V. Lombardi","doi":"10.1038/nsb0600_482","DOIUrl":"https://doi.org/10.1038/nsb0600_482","url":null,"abstract":"","PeriodicalId":18848,"journal":{"name":"Nature Structural Biology","volume":"479 1","pages":"482-485"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77050081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 101
期刊
Nature Structural Biology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1