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Control of pig reproduction XI 猪繁殖控制十一
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-31 DOI: 10.1002/mrd.23696
Clay A. Lents, Nicoline M. Soede

11th International Conference on Pig Reproduction

5th – 7th June 2023

Ghent Belgium

The 11th International Conference on Pig Reproduction (ICPR) was held in the beautiful city of Ghent, Belgium, on June 5-7 in 2023. Former ICPR meetings had always taken place at 4-year intervals, but the worldwide epidemic of Corona-19 forced us to a 2-year delay, and we are thankful that we could again meet to discuss pig reproduction. The program of the meeting was developed by the International Organizing Committee. The contributions of 26 invited speakers focused on new insights into specific reproductive processes in female and male pigs and their gametes, and on genetic, environmental or management factors influencing these processes. A total of 74 posters were presented, of which 14 were selected to deliver an oral presentation, and 26 gave a flash presentation, which was a first at an ICPR meeting but represents a continued commitment of ICPR to trainee and student development.

The success of the 11th ICPR was due to the hard work and commitment of the local organising committee, chaired by Dr Dominiek Maes. The conference ran smoothly and was enjoyed by the delegates, providing a forum for stimulating scientific exchange with colleagues old and new. The conference attracted over 130 delegates from at least 18 countries, and was funded, in part, by the USDA National Institute of Food and Agriculture (Agriculture and Food Research Initiative Competitive Grant no. 2021-67015-33405). An outstanding meeting was further made possible by many industry and academic sponsors. The detailed program can be found at https://icpr2023.be.

Proceedings of previous ICPR meetings 1 through 9 can be found at www.biosciproceedings.org. Proceedings of the 10th ICPR were published as a special issue of Molecular Reproduction and Development (MRD) and are available at https://onlinelibrary.wiley.com/toc/10982795/2017/84/9. The ICPR is happy to again partner with MRD for the current proceedings entitled Control of Pig Reproduction XI. It contains review articles accompanying the invited lectures that have been internationally peer-reviewed, and abstracts of the posters and oral presentations that were peer-reviewed by the local and international organizing committees. Sincere thanks are due to the many external scientific reviewers, MRD editorial board members, and ICPR co-editors Inma Parrilla and Agnieszka Waclawik. The ICPR thanks Harvey Florman, Eunice Precious Jasmine D and Ekta Kumar at MRD for their hard work and commitment. We are confident that this Special Issue will provide a valuable resource for everyone interested in pig reproduction and fertility.

第11届国际猪繁殖会议(ICPR)于2023年6月5日至7日在比利时美丽的根特市举行。以前的ICPR会议总是每4年举行一次,但全球范围内的冠状病毒-19的流行迫使我们推迟了2年,我们很高兴我们能再次开会讨论猪的繁殖问题。会议的日程是由国际组委会制定的。26位受邀演讲者的贡献集中在对母猪和雄性猪及其配子的特定生殖过程的新见解,以及影响这些过程的遗传、环境或管理因素。共展出74张海报,其中14张海报被选中作口头介绍,26张海报作速写介绍,这是ICPR会议上的第一次,但代表ICPR对实习生和学生发展的继续承诺。第十一届ICPR的成功是由多米尼克·梅斯博士担任主席的当地组委会的辛勤工作和承诺的结果。会议进行得很顺利,代表们都很喜欢,为新老同事之间的科学交流提供了一个平台。这次会议吸引了来自至少18个国家的130多名代表参加,会议的部分资金由美国农业部国家粮食和农业研究所(农业和食品研究计划竞争补助金号)提供。2021-67015-33405)。许多行业和学术赞助商进一步使这次会议成为可能。ICPR 1 ~ 9届会议的详细日程可以在https://icpr2023.be.Proceedings上找到,可以在www.biosciproceedings.org上找到。第十届ICPR会议记录作为《分子生殖与发育》(MRD)的特刊发表,可在https://onlinelibrary.wiley.com/toc/10982795/2017/84/9上查阅。它包含已经过国际同行评议的邀请讲座的评论文章,以及经当地和国际组委会同行评议的海报和口头报告的摘要。衷心感谢众多外部科学审稿人、MRD编辑委员会成员以及ICPR共同编辑Inma Parrilla和Agnieszka Waclawik。ICPR感谢MRD的Harvey Florman、Eunice Precious Jasmine D和Ekta Kumar的辛勤工作和承诺。我们相信,这期特刊将为所有对猪的繁殖和生育感兴趣的人提供宝贵的资源。
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引用次数: 0
ICPR ABSTRACTS ICPR摘要
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-31 DOI: 10.1002/mrd.23697
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引用次数: 0
Metabolomic evolution of the postpartum dairy cow uterus 产后奶牛子宫代谢组的演变
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-26 DOI: 10.1002/mrd.23702
Nicolas Aranciaga, Alastair B. Ross, James D. Morton, Robin McDonald, Jessica L. Gathercole, Debra K. Berg

High rates of early pregnancy loss are a critical issue in dairy herds, particularly in seasonal, grazing systems. Components of the uterine luminal fluid (ULF), on which the early embryo depends for sustenance and growth, partly determine early pregnancy losses. Here, changes in ULF from early to mid-postpartum in crossbred dairy cows were explored, linking them with divergent embryo development. For this, the uteri of 87 cows at Day 7 of pregnancy at first and third estrus postpartum were flushed to collect ULF. Eighteen metabolites (chiefly organic acids and sugars) significantly varied in abundance across postpartum, indicating a molecular signature of physiological recovery consistent of the upregulation of pyrimidine metabolism and glycerophospholipid metabolism, and downregulation of pentose phosphate and taurine metabolism pathways. Joint pathway analysis of metabolomics data and a previously generated proteomics data set on the same ULF samples suggests key links between postpartum recovery and subsequent successful embryo development. These include upregulation of VEGFA and downregulation of metabolism, NRF2, T-cell receptor, which appear to improve the ULF's capacity of sustaining normal embryo development, and a putative osmo-protectant role of beta-alanine. These relationships should be further investigated to develop tools to detect and reduce early pregnancy loss in dairy cows.

早孕损失率高是奶牛场的一个关键问题,尤其是在季节性放牧系统中。早期胚胎赖以生存和成长的子宫腔液(ULF)的成分在一定程度上决定了早期妊娠损失。在此,我们探讨了杂交奶牛产后早期到中期ULF的变化,并将其与胚胎发育差异联系起来。为此,对 87 头怀孕第 7 天、产后第一次和第三次发情的奶牛的子宫进行了冲洗,以收集超低频。18种代谢物(主要是有机酸和糖)的丰度在产后有显著差异,表明生理恢复的分子特征与嘧啶代谢和甘油磷脂代谢的上调以及磷酸戊糖和牛磺酸代谢途径的下调一致。对代谢组学数据和先前在相同超短波样品上生成的蛋白质组学数据集进行的联合通路分析表明,产后恢复与随后胚胎的成功发育之间存在关键联系。其中包括血管内皮生长因子(VEGFA)的上调和新陈代谢、NRF2、T 细胞受体的下调,这似乎提高了超微结构维持正常胚胎发育的能力,以及 beta-丙氨酸的潜在渗透保护作用。应进一步研究这些关系,以开发检测和减少奶牛早期妊娠损失的工具。
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引用次数: 0
Participation of apoptotic markers in the process of maturation and elimination of spermatozoa in the epididymis of the Corynorhinus mexicanus bat 细胞凋亡标志物参与墨西哥绿僵菌蝙蝠附睾精子的成熟和消除过程。
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-11 DOI: 10.1002/mrd.23701
Gihovani Ademir Samano-Barbosa, Blanca Patrícia López-Trinidad, Ahiezer Rodríguez-Tobón, Julio Cesar Chávez-Zamora, Edith Cortés-Barberena, Miguel Angel León-Galván, Ernesto Rodríguez-Tobón, Edith Arenas-Ríos

The Corynorhinus mexicanus bat is characterized by a specific form of reproductive asynchrony between males and females. After mating, some sperm remain in the male's epididymis, the organ where the sperm had matured. It has not yet been determined if apoptotic markers participate in the process of the maturation and/or elimination of these cells, so studying this topic is essential for our understanding of this species. Male bats were collected during three stages: Before mating; during the Mating phase; After mating and the final phase, which we call, Storage. Their epididymides were removed, weighed and measured. Sperm were extracted and the following sperm parameters were evaluated: active caspases, phosphatidylserine externalization, and mitochondrial membrane potential. Sperm from the testes enter the epididymis during Before mating, causing the organ to grow. During Mating phase, spermatozoa present a large amount of active caspases with externalization of phosphatidyl serine, even while still alive. This suggests that these two markers could participate in maturation and elimination, respectively.

墨西哥Corynorhinus蝙蝠的特征是雄性和雌性之间存在一种特定形式的生殖不同步。交配后,一些精子留在雄性的附睾中,附睾是精子成熟的器官。目前还没有确定凋亡标志物是否参与了这些细胞的成熟和/或消除过程,因此研究这一主题对我们理解这一物种至关重要。雄蝙蝠在三个阶段被采集:交配前;在交配阶段;交配后和最后阶段,我们称之为储存。取下附睾,称重并测量。提取精子并评估以下精子参数:活性胱天蛋白酶、磷脂酰丝氨酸外化和线粒体膜电位。睾丸中的精子在交配前进入附睾,使器官生长。在交配期,精子即使还活着,也会表现出大量具有磷脂酰丝氨酸外化的活性半胱天冬酶。这表明这两种标记物可能分别参与成熟和消除。
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引用次数: 0
lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia lncRNA GHET1通过EZH2/LSD1介导的子痫前期MT2A表观遗传学抑制调节绒毛外滋养层表型。
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-07 DOI: 10.1002/mrd.23693
Pengyun Wan, Jia Huang, Wenting Liu, Xiaoyan Su, Bei Zhao, Xianggang Wang, Lu Zhao

Pre-eclampsia (PE) is usually defined as new-onset hypertension with albuminuria or other organ damage. Herein, the role and mechanism of long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) during PE are investigated. Expression of GHET1 in PE pregnancies was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and cell cycle of extravillous trophoblasts were assessed by Cell Counting Kit-8 (CCK-8), colony formation, 5-Ethynyl-2′-deoxyuridine (EdU) assays, and flow cytometry, respectively. Migration, invasion, and network formation of trophoblasts were measured by wound healing, transwell system, and tube formation assays. RNA immunoprecipitation (RIP), RNA pull-down, and chromatin immunoprecipitation (ChIP) assays were used to confirm the molecular interaction. GHET1 was markedly decreased in the placenta of PE patients. GHET1 promoted the proliferation and cell cycle of extravillous trophoblasts, as well as migration, invasion, and network formation in vitro. Metallothionein 2A (MT2A) functioned as a downstream effector of GHET1, which was negatively correlated with GHET1 in PE. GHET1 directly bound with zeste 2 polycomb repressive complex 2/lysine-specific demethylase 1 (EZH2/LSD1). Knockdown of GHET1 reduced the occupancies of H3K27me3 and H3K4me2 in the MT2A promoter region by recruiting EZH2 and LSD1. MT2A knockdown reversed GHET1 inhibition mediated biological functions. GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in PE.

先兆子痫(PE)通常被定义为伴有蛋白尿或其他器官损伤的新发性高血压。本文探讨了长链非编码RNA(lncRNA)胃癌高表达转录物1(GHET1)在PE中的作用及其机制。应用实时定量聚合酶链反应(qRT-PCR)评估PE妊娠中GHET1的表达。分别通过细胞计数试剂盒-8(CCK-8)、集落形成、5-乙炔基-2'-脱氧尿苷(EdU)测定和流式细胞术评估绒毛外滋养层的增殖和细胞周期。滋养层细胞的迁移、侵袭和网络形成通过伤口愈合、transwell系统和试管形成测定进行测量。RNA免疫沉淀(RIP)、RNA下拉和染色质免疫沉淀(ChIP)分析用于确认分子相互作用。GHET1在PE患者胎盘中明显降低。GHET1在体外促进绒毛外滋养层细胞的增殖和细胞周期,以及迁移、侵袭和网络形成。金属硫蛋白2A(MT2A)是GHET1的下游效应子,与PE中的GHET1呈负相关。GHET1的敲除通过募集EZH2和LSD1降低了H3K27me3和H3K4me2在MT2A启动子区的占有率。MT2A敲低逆转了GHET1抑制介导的生物功能。GHET1通过EZH2/LSD1介导的PE中MT2A表观遗传学抑制调节绒毛外滋养层表型。
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引用次数: 0
Inhibition of phosphodiesterase PDE8B reduces activation of primordial follicles in mouse ovaries 抑制磷酸二酯酶PDE8B可降低小鼠卵巢中原始卵泡的激活
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-07-27 DOI: 10.1002/mrd.23699
Julie Feld Madsen, Mahboobeh Amoushahi, Christian Posselt Choi, Stine Bundgaard, Anders Heuck, Karin Lykke-Hartmann

In the ovaries, cyclic adenosine 3′,5′-monophosphate (cAMP) is a second messenger supporting the generation of steroids. Phosphodiesterases (PDEs) are regulators of intracellular cAMP, and therefore, potential regulators of ovarian function. Interestingly, the family of PDE genes are differentially expressed in human oocytes and granulosa cells from primordial and primary follicles, suggesting diverse roles. In this study, we addressed the functions of PDE3B and PDE8B in primordial follicle regulation using inhibitors of PDE3B and PDE8B in murine ovary primary in vitro cultures. Inhibition of PDE8B in ovarian cultures prevented primordial follicle activation, while inhibition of PDE3B had no effect on follicle distribution in the ovary, under the tested conditions. As cAMP levels may increase steroid levels, we assessed the protein levels of the steroidogenic acute regulatory protein (StAR) and aromatase enzymes, and found that inhibition of PDE3B reduced StAR protein levels, whereas inhibition of PDE8 did not alter StAR expression in our murine ovary culture system conditions. Our results showed that ketotifen-induced inhibition of PDE8B can decrease primordial follicle activation, whereas we observed no effect of follicle distribution, when PDE3B was inhibited. Expression of the StaR enzyme was not altered when PDE8B was inhibited, which might reflect not sufficient inhibition by ketotifen to induce StAR alterations, or redundant mechanisms.

在卵巢中,环腺苷3′,5′-单磷酸(cAMP)是支持类固醇生成的第二信使。磷酸二酯酶是细胞内cAMP的调节因子,因此也是卵巢功能的潜在调节因子。有趣的是,PDE基因家族在原始卵泡和初级卵泡的人类卵母细胞和颗粒细胞中差异表达,表明其作用不同。在本研究中,我们使用PDE3B和PDE8B抑制剂在小鼠卵巢原代体外培养中研究了PDE3B或PDE8B在原始卵泡调节中的功能。在测试条件下,抑制卵巢培养物中的PDE8B阻止了原始卵泡的激活,而抑制PDE3B对卵泡在卵巢中的分布没有影响。由于cAMP水平可能会增加类固醇水平,我们评估了类固醇生成急性调节蛋白(StAR)和芳香化酶的蛋白水平,发现抑制PDE3B降低了StAR蛋白水平,而抑制PDE8不会改变小鼠卵巢培养系统条件下StAR的表达。我们的结果表明,酮替芬诱导的PDE8B抑制可以降低原始卵泡的激活,而当PDE3B被抑制时,我们没有观察到卵泡分布的影响。当PDE8B被抑制时,StaR酶的表达没有改变,这可能反映出酮替芬没有足够的抑制作用来诱导StaR的改变,或者冗余机制。
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引用次数: 0
The expression levels of NOS2, HMOX1, and VEGFC in cumulus cells are markers of oocyte maturation and fertilization rate 卵丘细胞中NOS2、HMOX1和VEGFC的表达水平是卵母细胞成熟和受精率的标志
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-07-24 DOI: 10.1002/mrd.23698
Montserrat Barragán, David Cornet-Bartolomé, Natalia Molina, Rita Vassena

Throughout the reproductive life of women, cumulus cells (CC) protect the dormant oocyte from damage, act as sensors of the follicular microenvironment, and act as a gatekeeper for oocyte developmental potential. One such mechanism relies on the hypoxia-tolerance response, which, with age, decreases systematically, including in the ovary. We aimed to evaluate the association between gene expression related to hypoxia and aging in CC and reproductive results in in vitro fertilization cycles. We recruited 94 women undergoing controlled ovarian stimulation. Total RNA was extracted from pooled CCs collected after oocyte pick-up (OPU) and reverse-transcribed to complementary DNA using random hexamers to test 14 genes related to hypoxia response via HIF1α activation, oxidative stress, and angiogenic responses. The expression of CLU, NOS2, and TXNIP had a positive correlation with age (rs = 0.25, rs = 0.24, and rs = 0.35, respectively). Additionally, NOS2 and HMOX1 expression correlated positively with the retrieval of immature oocytes (rs = 0.22 and rs = 0.40, respectively). Moreover, VEGFC levels decreased overall with increasing fertilization rate, independently of age (rs = −0.29). We found that the fertilization potential of a cohort of oocytes is related to the ability of CC to respond to oxidative stress and hypoxia with age, pointing at NOS2, HMOX1, and VEGFC expression as markers for oocyte maturation and fertilization success.

在女性的整个生殖生活中,卵丘细胞(CC)保护休眠的卵母细胞免受损伤,充当卵泡微环境的传感器,并充当卵母细胞发育潜力的守门人。其中一种机制依赖于耐缺氧反应,随着年龄的增长,耐缺氧反应会系统性降低,包括在卵巢中。我们旨在评估CC中与缺氧和衰老相关的基因表达与体外受精周期中生殖结果之间的关系。我们招募了94名接受控制性卵巢刺激的女性。从卵母细胞采集(OPU)后收集的汇集CC中提取总RNA,并使用随机六聚体逆转录为互补DNA,以测试通过HIF1α激活、氧化应激和血管生成反应与缺氧反应相关的14个基因。CLU、NOS2和TXNIP的表达与年龄呈正相关(rs = 0.25,rs = 0.24和rs = 0.35)。此外,NOS2和HMOX1的表达与未成熟卵母细胞的回收呈正相关(rs = 0.22和rs = 0.40)。此外,VEGFC水平总体上随着受精率的增加而下降,与年龄无关(rs = −0.29)。我们发现,一组卵母细胞的受精潜力与CC随着年龄的增长对氧化应激和缺氧的反应能力有关,NOS2、HMOX1和VEGFC的表达是卵母细胞成熟和受精成功的标志。
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引用次数: 0
Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3 Hsa_circ_0001740通过调节miR-188-3p/ARRDC3介导滋养细胞功能
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-07-12 DOI: 10.1002/mrd.23695
Mei Long, Shan Wang

Preeclampsia is an obstetric disorder and remains the leading contributor to maternal and fetal morbidity and mortality. This study was designed to explore the role of hsa_circ_0001740 in preeclampsia as well as its underlying mechanism. Real-time quantitative polymerase chain reaction was performed to examine hsa_circ_0001740 and miR-188-3p levels in trophoblast cell line HTR-8/SVneo. The proliferation, migration, invasion, and apoptosis of HTR-8/SVneo cells were detected using cell counting kit-8, colony formation, wound healing, transwell, and terminal-deoxynucleoitidyl transferase mediated nick end labeling assays, respectively. The expression of apoptosis- and Hippo signaling-related proteins were assessed by western blot. Moreover, the binding relationship between hsa_circ_0001740 and miR-188-3p, miR-188-3p and ARRDC3 were verified by luciferase report assay. The results showed that hsa_circ_001740 overexpression inhibited the proliferation, migration, and invasion, and promoted apoptosis of HTR-8/SVneo cells. Hsa_circ_0001740 was verified to bind to miR-188-3p, and ARRDC3 was demonstrated to be a target of miR-188-3p. miR-188-3p overexpression partially counteracted the suppressive effects of hsa_circ_001740 overexpression on the proliferation, migration, and invasion of HTR-8/SVneo cells. What's more, ARRDC3 expression was upregulated by hsa_circ_001740-overexpression but was downregulated by miR-188-3p overexpression. Hsa_circ_001740/miR-188-3p also mediated Hippo signaling. To summarize, hsa_circ_0001740 could maintain trophoblast cell function via downregulating miR-188-3p, providing a potential biomarker for the diagnosis and treatment of preeclampsia.

子痫前期是一种产科疾病,仍然是孕产妇和胎儿发病率和死亡率的主要原因。本研究旨在探讨hsa_circ_0001740在子痫前期的作用及其潜在机制。采用实时定量聚合酶链反应检测滋养细胞HTR-8/SVneo中hsa_circ_0001740和miR-188-3p水平。分别采用细胞计数试剂盒-8、菌落形成、伤口愈合、transwell和末端脱氧核苷转移酶介导的缺口末端标记法检测HTR-8/SVneo细胞的增殖、迁移、侵袭和凋亡。western blot检测细胞凋亡相关蛋白和Hippo信号相关蛋白的表达。此外,hsa_circ_0001740与miR-188-3p、miR-188-3p与ARRDC3的结合关系通过荧光素酶报告实验验证。结果表明,hsa_circ_001740过表达可抑制HTR-8/SVneo细胞的增殖、迁移和侵袭,促进细胞凋亡。Hsa_circ_0001740被证实与miR-188-3p结合,ARRDC3被证明是miR-188-3p的靶标。miR-188-3p过表达部分抵消了hsa_circ_001740过表达对HTR-8/SVneo细胞增殖、迁移和侵袭的抑制作用。hsa_circ_001740过表达可上调ARRDC3的表达,miR-188-3p过表达可下调ARRDC3的表达。Hsa_circ_001740/miR-188-3p也介导Hippo信号。综上所述,hsa_circ_0001740可以通过下调miR-188-3p来维持滋养细胞功能,为子痫前期的诊断和治疗提供了潜在的生物标志物。
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引用次数: 0
Decreased LONP1 expression contributes to DNA damage and meiotic defects in oocytes 降低LONP1表达有助于卵母细胞DNA损伤和减数分裂缺陷
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-07-01 DOI: 10.1002/mrd.23694
Chuanming Liu, Manlin Xu, Yajie Guan, Lilin Li, Wenwen Liu, Bichun Guo, Xiaoqiang Sheng, Yang Zhang, Jidong Zhou, Xin Zhen, Guijun Yan, Haixiang Sun, Lijun Ding

Meiotic defects in oocytes are the primary reason for decreased female fertility with advanced maternal age. In this study, we revealed that decreased expression of ATP-dependent Lon peptidase 1 (LONP1) in aged oocytes and oocyte-specific depletion of LONP1 disrupt oocyte meiotic progression accompanying with mitochondrial dysfunction. In addition, LONP1 downregulation increased oocyte DNA damage. Moreover, we demonstrated that splicing factor proline and glutamine rich directly interacts with LONP1 and mediate the effect of LONP1 depletion on meiotic progression in oocytes. In summary, our data suggest that decreased expression of LONP1 is involved in advanced maternal age-related meiosis defects and that LONP1 represents a new therapeutic target to improve aged oocyte quality.

卵母细胞的减数分裂缺陷是高龄产妇生育能力下降的主要原因。在这项研究中,我们发现,衰老卵母细胞中ATP依赖性Lon肽酶1(LONP1)的表达减少和卵母细胞特异性LONP1的缺失破坏了卵母细胞减数分裂进程,并伴有线粒体功能障碍。此外,LONP1下调增加了卵母细胞DNA损伤。此外,我们证明了富含脯氨酸和谷氨酰胺的剪接因子直接与LONP1相互作用,并介导LONP1缺失对卵母细胞减数分裂进程的影响。总之,我们的数据表明,LONP1表达的减少与晚期母体年龄相关的减数分裂缺陷有关,并且LONP1代表了改善衰老卵母细胞质量的一个新的治疗靶点。
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引用次数: 0
The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells 组成活性的pSMAD2/3相对促进鸡原始生殖细胞的增殖
IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-06-28 DOI: 10.1002/mrd.23689
Masumeh Zare, Seyed Ziaeddin Mirhoseini, Shahrokh Ghovvati, Saeed Yakhkeshi, Mahdi Hesaraki, Mojgan Barati, Forough Azam Sayyahpour, Hossein Baharvand, Seyedeh-Nafiseh Hassani

In many multicellular organisms, mature gametes originate from primordial germ cells (PGCs). Improvements in the culture of PGCs are important not only for developmental biology research, but also for preserving endangered species, and for genome editing and transgenic animal technologies. SMAD2/3 appear to be powerful regulators of gene expression; however, their potential positive impact on the regulation of PGC proliferation has not been taken into consideration. Here, the effect of TGF-β signaling as the upstream activator of SMAD2/3 transcription factors was evaluated on chicken PGCs' proliferation. For this, chicken PGCs at stages 26−28 Hamburger−Hamilton were obtained from the embryonic gonadal regions and cultured on different feeders or feeder-free substrates. The results showed that TGF-β signaling agonists (IDE1 and Activin-A) improved PGC proliferation to some extent while treatment with SB431542, the antagonist of TGF-β, disrupted PGCs' proliferation. However, the transfection of PGCs with constitutively active SMAD2/3 (SMAD2/3CA) resulted in improved PGC proliferation for more than 5 weeks. The results confirmed the interactions between overexpressed SMAD2/3CA and pluripotency-associated genes NANOG, OCT4, and SOX2. According to the results, the application of SMAD2/3CA could represent a step toward achieving an efficient expansion of avian PGCs.

在许多多细胞生物中,成熟配子起源于原始生殖细胞(PGCs)。PGCs培养的改进不仅对发育生物学研究很重要,而且对保护濒危物种、基因组编辑和转基因动物技术也很重要。SMAD2/3似乎是基因表达的强大调节因子;然而,它们对PGC增殖调节的潜在积极影响尚未被考虑在内。在此,评估了TGF-β信号作为SMAD2/3转录因子的上游激活剂对鸡PGCs增殖的影响。为此,从胚胎性腺区域获得26−28 Hamburger−Hamilton阶段的鸡PGCs,并在不同的饲养物或无饲养物的基质上培养。结果表明,TGF-β信号激动剂(IDE1和激活素-A)在一定程度上改善了PGC的增殖,而TGF-β的拮抗剂SB431542则破坏了PGCs的增殖。然而,用组成型活性SMAD2/3(SMAD2/3CA)转染PGC导致PGC增殖改善5周以上。结果证实了过表达的SMAD2/3CA与多能性相关基因NANOG、OCT4和SOX2之间的相互作用。根据研究结果,SMAD2/3CA的应用可能代表着实现鸟类PGCs有效扩增的一步。
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引用次数: 0
期刊
Molecular Reproduction and Development
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