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The Positive Effect of Lithium for Elevated Specific Activities of Alkaline Phosphatase and β-Galactosidase on the Liver and Brain Cell Membrane Integrity in Young and Old Aged Rats 锂对提高碱性磷酸酶和β-半乳糖苷酶比活性对青年和老年大鼠肝和脑细胞细胞膜完整性的积极影响
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/13273d
M. Prabha, K. N. Sushma, P. Suneetha, R. S. Kiran
Objectives: Differentiate the particular activity of Alkaline Phosphatase and (beta)-Galactosidase in LiCl2 treated young and old aged rat's brain and liver to their control. Materials and Methods: For 10 and 20 days, four groups of male wistar rats (each n=5) were used to assess the levels of Alkaline phosphatase and (beta)-galactosidase. In comparison to the control, LiCl2 treated the brain and liver of young and old rats. Results: LiCl2 increases protein content, which is required for old brain cell activation. Alkaline phosphatase and (beta)-Galactosidase showed increased specific activity in the brain and liver of young and old rats treated with LiCl2 for 10 and 20 days, respectively, when compared to their control. In fact Alkaline phosphatase showed significantly higher activity for 10 and 20 days LiCl2 treated young brain exhibited 16.77 ± 0.309 and 27.74 ± 0.237 respectively as compared to their control 3.80 ± 0.230 while old rat's brain exhibited 14.28 ± 0.215 and 16.30 ± 0.24 respectively as compared to their control 8.65 ± 0.304 nm/min/mg of protein, similarly higher activity obtained for young liver 19.51 ±0.487 and 21.28 ± 0.317 as compared to their control 3.67 ± 0.402 and finally the old liver exhibited 15.02 ± 0.355 and 18.45 ± 0.439 as compared to their control 3.26 ± 0.304 nm/min/mg of protein respectively. Conclusion: These findings confirm that both membrane bound Alkaline Phosphatase and (beta)-Galactosidase enzymes exhibited higher specific activity for LiCl2 treatment in wistar rats brain and liver cells. So lithium has a positive effect on both young and old brain cells for (beta)-galactosidase while having a greater positive effect on Alkaline phosphatase in the rat liver and brain for cell membrane integrity.
目的:区分LiCl2处理的幼龄和老年大鼠脑和肝脏中碱性磷酸酶和(beta) -半乳糖苷酶的特异性活性。材料与方法:取4组雄性wistar大鼠,每组5只,分别于10和20 d测定其碱性磷酸酶和(beta) -半乳糖苷酶水平。与对照组相比,LiCl2治疗了年轻和年老大鼠的大脑和肝脏。结果:LiCl2增加了老年脑细胞活化所需的蛋白质含量。与对照组相比,LiCl2处理10天和20天的年轻和年老大鼠脑和肝脏中碱性磷酸酶和(beta) -半乳糖苷酶的特异性活性分别有所增加。事实上,LiCl2处理后10天和20天的碱性磷酸酶活性显著高于对照组(3.80±0.230),分别为16.77±0.309和27.74±0.237;老龄大鼠脑活性分别为14.28±0.215和16.30±0.24,分别高于对照组(8.65±0.304 nm/min/mg)。与对照组(3.67±0.402)相比,年轻肝脏(19.51±0.487)和21.28±0.317)的蛋白质活性同样较高;老年肝脏(15.02±0.355)和18.45±0.439)的蛋白质活性分别高于对照组(3.26±0.304)nm/min/mg。结论:这些发现证实了膜结合碱性磷酸酶和(beta) -半乳糖苷酶对LiCl2处理在wistar大鼠脑和肝细胞中具有较高的特异性活性。因此,锂对年轻和年老的脑细胞(beta) -半乳糖苷酶都有积极作用,而对大鼠肝脏和大脑的碱性磷酸酶对细胞膜完整性有更大的积极作用。
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引用次数: 0
Determination of Some Biochemical Indices in Catfish from Rivers, Swamp and Commercial Fish Ponds in Oil and Non-oil Polluted Areas in Rivers and Anambra States, Nigeria: A Comparative Assessment 尼日利亚河流和阿南布拉州石油和非石油污染地区河流、沼泽和商业鱼塘鲶鱼的一些生化指标测定:比较评估
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/4808f
F. Anacletus, G. E. Bekibele, S. Onuoha, K. Nwauche
This study intended to comparatively assess some biochemical indices in eighteen catfish harvested from rivers, swamp and commercial fish ponds in oil-polluted and non-oil polluted areas of Nigeria. Three fishes were collected from each of the following sites; Akaraolu swamps in Ahoada East LGA (AR), a commercial fish pond within the area (AP), The New Calabar River in Ikwerre LGA (BR), a fish pond within the area (BP), Omambala River, Anambra East LGA (CR), a fish pond in Awka, Anambra state (CP), six groups in all. Variation of effects in fishes from the two regions was estimated using the following biochemical parameters; alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine, urea, sodium ion, potassium ion, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT). Analysis was done in keeping with standard procedures. On analysis, the ALP and ALT activities (33.93±2.37 and 44.33±2.96 respectively) from AR were significantly (p<0.05) different from BR (17.00±0.58 and 35.00±5.69 respectively) and CR (22.00±2.31 and 30.67±2.60). The AST activity from AR (68.67±0.88) was not significantly different from that of BR (69.00±1.73) but was significantly different from that of CR (70.33±4.33). Also, site AP (65.53±0.37, 73.00±2.08 and 50.00±1.15 respectively) were significantly higher than CP (42.00±2.89, 64.67±4.33 and 44.67±5.46 respectively) and BP (26.00±1.15 and 50.20±1.15 respectively). However, the AST activity of catfish from AP (73.00±2.08) was not significantly different from that in BP (73.67±1.86). The creatinine, urea, Na+ and K+ concentrations observed from AR and AP were significantly higher in comparison to other sites. There was a significantly increased GSH, Catalase, and MDA, and a lower SOD concentration for AR and AP in comparison to other sites. The results show a variation in biochemical indices in fishes from oil-polluted sites when compared to non-oil polluted sites.
本研究旨在对尼日利亚石油污染地区和非石油污染地区的河流、沼泽和商业鱼塘中收获的18条鲶鱼的一些生化指标进行比较评价。在下列地点各收集了三条鱼;Akaraolu沼泽位于Ahoada East LGA (AR),区域内的一个商业鱼塘(AP), Ikwerre LGA的新卡拉巴尔河(BR),区域内的一个鱼塘(BP), Omambala河,阿南布拉东部LGA (CR),阿南布拉州Awka的一个鱼塘(CP),共六个群体。使用以下生化参数估计了两个地区鱼类的影响差异:丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、肌酐、尿素、钠离子、钾离子、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)。分析按照标准程序进行。分析显示,AR组ALP和ALT活性(分别为33.93±2.37和44.33±2.96)显著高于BR组(分别为17.00±0.58和35.00±5.69)和CR组(分别为22.00±2.31和30.67±2.60)。AR组AST活性(68.67±0.88)与BR组(69.00±1.73)差异不显著,与CR组(70.33±4.33)差异显著。AP(65.53±0.37、73.00±2.08、50.00±1.15)显著高于CP(42.00±2.89、64.67±4.33、44.67±5.46)和BP(26.00±1.15、50.20±1.15)。而AP中AST活性(73.00±2.08)与BP中AST活性(73.67±1.86)差异不显著。AR和AP部位肌酸酐、尿素、Na+和K+浓度显著高于其他部位。与其他部位相比,GSH、过氧化氢酶和MDA显著增加,AR和AP的SOD浓度较低。结果表明,与非石油污染区相比,石油污染区鱼类的生化指标存在差异。
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引用次数: 0
Effect of Post-fermentation Maceration on the Chemical Composition of Some Wines 发酵后浸渍对某些葡萄酒化学成分的影响
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/12691d
Nino G. Vephkhishvili, M. Bezhuashvili, Vano L. Shiukashvili
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引用次数: 0
Determining Antioxidant Activity in Honey as a Result of Haber-Wais Reaction 用Haber-Wais反应测定蜂蜜的抗氧化活性
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/14087d
A. Kesić, Nadira Ibrišimović-Mehmedinović, Almir Šestan, Aida Crnkić, B. Catovic
The antioxidants present in natural food products show a higher antioxidant activity than synthetic one [1]. Polyphenolic compounds are the most important antioxidants in bee honey. Bee honey is the most popular bee product by human [2]. In addition to the polyphenols there are non-phenolic compounds with antioxidant potential, such as proteins, gluconic acid, L-ascorbic acid, hydrogen peroxide, hydroxyl methyl furfural (HMF), Maillard reaction products, etc. The antioxidant activity of honey largely depends on the content of micro biogenic elements. The aims of this paper are to present the complex role of micro biogenic elements in the human. It is especially important to point out micro biogenic elements that can act both as antioxidants and as pro-oxidants, such as Cu. Cu acts as antioxidant because it is an integral part of some enzymes involved in the antioxidant activity of the organism. Cu in honey also acts as a pro-oxidant through Haber-weis reaction with L-ascorbic acid also present in honey. In addition to being an essential micro-nutrient Cu is potentially very hazardous because of the capacity change in oxidation state leading to the initiation of the reaction in which free radicals are formed. Therefore, the balanced intake of copper and other trace elements in the human body is very important. Therefore, changes in the antioxidant activity of food are the result of chemical changes in antioxidant active compounds present in the food product.
天然食品中的抗氧化剂表现出比合成抗氧化剂更高的抗氧化活性[1]。多酚类化合物是蜂蜜中最重要的抗氧化剂。蜂蜜是人类最喜爱的蜂产品[2]。除了多酚类化合物外,还有一些具有抗氧化潜力的非酚类化合物,如蛋白质、葡萄糖酸、l -抗坏血酸、过氧化氢、羟甲基糠醛(HMF)、美拉德反应产物等。蜂蜜的抗氧化活性在很大程度上取决于微生物元素的含量。本文的目的是介绍微生元素在人体中的复杂作用。特别重要的是要指出既可以作为抗氧化剂又可以作为促氧化剂的微量元素,如铜。铜具有抗氧化剂的作用,因为它是参与机体抗氧化活性的一些酶的组成部分。蜂蜜中的铜还通过与蜂蜜中存在的l -抗坏血酸的哈伯-威斯反应起到促氧化剂的作用。铜除了是一种必需的微量营养素外,还具有潜在的危险性,因为它在氧化状态下的容量变化会引发自由基形成的反应。因此,均衡摄入铜等人体微量元素是非常重要的。因此,食品抗氧化活性的变化是食品中抗氧化活性化合物发生化学变化的结果。
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引用次数: 0
Study on Phonon Anharmonicity in a Framework Material H3[Co(CN)6] 框架材料H3[Co(CN)6]中声子非调和性的研究
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/14409d
K. Mishra
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引用次数: 0
Study on Design, Synthesis of Novel 1, 8-Naphthalimidederivatives and Their Antimicrobial Activity 新型1,8 -萘酰亚胺衍生物的设计、合成及其抗菌活性研究
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/13557d
Rambabu Sirgamalla, Shyam Perka, Mukunda Annimalla
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引用次数: 0
Discovery of Small Compounds that Target FOXO Transcription Factors and Modulate their Transcriptional Activity and Physiological Function 靶向FOXO转录因子并调节其转录活性和生理功能的小化合物的发现
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/14349d
J. Hagenbuchner, V. Obsilova, P. Obexer, T. Obsil, M. Ausserlechner
FOXO transcription factors are critical regulators of cell homeostasis that control a plethora of different target genes and thereby steer cell death, differentiation, longevity and senescence in mammalian cells. The objective of the present study was to identify novel chemical compounds that bind to the DNA-binding domain of FOXO3 and modulate its transcriptional activity. The strategy was to combine pharmacophore-modelling-based in silico compound screening with fluorescence polarization protein-DNA binding assays and cell-based compound validation. By this approach, we identified small molecules that physically interact with the DNA-binding domain (DBD) of FOXO transcription factors with highest affinity to human FOXO3. These compounds do not act as chemical inhibitors, but modulate the FOXO3 transcriptional program in human cells. The mode of interaction between compounds and the FOXO3-DBD was assessed by NMR spectroscopy and docking studies. We demonstrate that compound S9 and its oxalate salt S9OX interfere with FOXO3 target promoter binding, modulate the transcription of distinct gene subsets and interfere with the physiological program activated by FOXO3 in cancer cells. These small molecules prove the druggability of the FOXO-DBD and provide a structural basis for modulating these important homeostasis regulators by directly affecting protein-DNA interaction in normal and malignant cells.
FOXO转录因子是细胞稳态的关键调节因子,控制着大量不同的靶基因,从而引导哺乳动物细胞的死亡、分化、寿命和衰老。本研究的目的是鉴定与FOXO3的dna结合域结合并调节其转录活性的新化合物。该策略是将基于药物团模型的硅化合物筛选与荧光偏振蛋白- dna结合试验和基于细胞的化合物验证相结合。通过这种方法,我们确定了与FOXO转录因子的dna结合域(DBD)物理相互作用的小分子,这些小分子与人类FOXO3的亲和力最高。这些化合物不作为化学抑制剂,但在人类细胞中调节FOXO3转录程序。通过核磁共振波谱和对接研究评估了化合物与FOXO3-DBD的相互作用模式。我们证明了化合物S9及其草酸盐S9OX干扰FOXO3靶启动子结合,调节不同基因亚群的转录,并干扰FOXO3在癌细胞中激活的生理程序。这些小分子证明了FOXO-DBD的可药物性,并通过直接影响正常和恶性细胞中的蛋白质- dna相互作用,为调节这些重要的稳态调节因子提供了结构基础。
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引用次数: 0
Kinetics and Isotherm Studies for Methylene Blue Dye Removal Using Carbon Derived from Bulb of Zephyranthes citrina 西风球茎碳去除亚甲基蓝染料的动力学和等温线研究
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/14433d
S. Vedanayaki, J. Prakash
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引用次数: 0
Studies on Colour Removal from Dye Aqueous Solution Using Modified Forms of Adsorbent Materials 改性吸附材料对染料水溶液脱色的研究
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/14117d
A. Geetha
The potentiality of activated carbon (CSAC) and polypyrrole coated sawdust (PPy/SD) derived from the fruit of the gardening plant material of cordia sebestena to remove acid orange -7 from aqucous solutions via adsorption was investigated. The adsorption process provides an attractive alternative treatment, especially if the adsorbent is inexpensive and readily available. The batch mode adsorption studies were carried out by varying experimental parameters such as pH, initial dye concentration, contact time and temperature. The kinetic data at different concentrations and at different temperatures were analysed for both the adsorbents using pseudo first order and pseudo second order models. The equilibrium data were also analysed by Freundlich and Langmuir models. Thermodynamic studies were also evaluated. Finally, the performance of CSAC was compared with PPy/SD. The experimental results indicated that PPy/SD is most suitable for the removal of Acid Orange 7 from aqueous solutions than CSAC.
研究了园艺植物天麻果实中的活性炭(CSAC)和聚吡咯包覆木屑(PPy/SD)吸附去除水中酸性橙-7的潜力。吸附过程提供了一种有吸引力的替代处理方法,特别是如果吸附剂便宜且容易获得。通过改变pH、初始染料浓度、接触时间和温度等实验参数,进行了间歇式吸附研究。采用拟一级和拟二级模型分析了两种吸附剂在不同浓度和不同温度下的动力学数据。平衡数据也用Freundlich和Langmuir模型分析。热力学研究也进行了评估。最后,比较了CSAC与PPy/SD的性能。实验结果表明,PPy/SD比CSAC更适合去除水溶液中的酸性橙7。
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引用次数: 0
Assessment of Physicochemical and Microbial Factors of Water in Lower Anaicut Basin, Tamil Nadu, India 印度泰米尔纳德邦下阿纳库特盆地水体理化和微生物因子评价
Pub Date : 2021-10-11 DOI: 10.9734/bpi/nicb/v4/1980c
P. Anbusrinivasan, M. Thenral
Water quality and quantity have an important impact in a region's health, wealth, and prosperity. Water pollution is a phenomenon that occurs when the quality of water deteriorates as a result of numerous human activities. This chapter provides basic idea of   physiochemical and microbial factors which gives sustainable environment for aquatic life.  In this chapter, the physico-chemical parameters and microbial population in soil sediment, water and gill region of Labeo rohita from Lower Anaicut Basin were examined. Based on their growth characteristics on specific culture media, some human bacterial and fungal pathogens were isolated. The fishes' medium and the sediments in their habitat were also collected and tested for pathogens. In the gill region, however, five bacterium species and four fungus species were discovered.This study focuses on the quality of Labeo rohita in the Lower Anaicut basin and aims to raise awareness among fish consumers.
水的质量和数量对一个地区的健康、财富和繁荣有着重要的影响。水污染是由于许多人类活动导致水质恶化而发生的一种现象。本章提供了提供水生生物可持续发展环境的理化和微生物因素的基本概念。本章对下阿纳库特盆地罗氏拉贝的土壤沉积物、水体和鳃区理化参数和微生物种群进行了研究。根据其在特定培养基上的生长特点,分离出了一些人类细菌和真菌病原体。还收集了鱼类的培养基和栖息地的沉积物并进行了病原体检测。在鳃区发现了5种细菌和4种真菌。本研究的重点是下阿纳库特盆地拉比奥罗希塔的质量,旨在提高鱼类消费者的认识。
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引用次数: 0
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New Innovations in Chemistry and Biochemistry Vol. 4
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