Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology最新文献

The total NDAs obtained from a clinically atypical molluscum contagiosum (MC) found in a 47-year-old, male immunosuppressed renal allograft recipient and from seven MC of usual infantile cases were digested with BamHI restriction enzyme and analyzed by agarose gel electrophoresis. Three different cleavage patterns were observed and tentatively named MCV-a, -b and -c. All the infantile cases were infected by either MCV-a or MCV-b, both of which showed similar cleavage patterns. In contrast, the immunosuppressed patient was infected by MCV-c, whose cleavage pattern was quite different from those of MCV-a and -b. These results clarified the heterogeneity of MCV in Japanese patients with MC and implied the correlation between MCV types and clinical features of MC.

On the basis of clinical and histopathological studies on 17 patients who had been diagnosed as having pigmented Spitz nevus (PSN), pigmented spindle cell nevus (PSCN) was surmised to be a type of pigmented Spitz nevus. In order to distinguish pigmented spindle cell nevus and pigmented Spitz nevus from early melanoma, 5 PSCN cases and 12 PSN cases were analyzed by the fine-needle aspiration fluorescence method, touch fluorescence method and measurement of the 5-S-CD level in the lesion. With the touch fluorescence method, fluorescent tumor cells were detected in one case of PSN. With the fine-needle aspiration fluorescence method, fluorescent tumor cells were detected in one PSCN case and 2 PSN cases. In comparison with fluorescent melanoma cells, the detected fluorescent tumor cells were smaller in size and number and resembled melanocytes. The 5-S-CD level in the lesion was 50 ng/mg or less in all cases, whereas the level in melanoma is known to be a high 100 ng/mg or more. In the final analysis, measurement of the 5-S-CD level in the lesion was concluded to have the greatest utility for differential diagnosis of pigmented spindle cell nevus and pigmented Spitz nevus from early melanoma.

A 22-year-old woman with ten-year history of atopic dermatitis first noticed an erythematous ++, indurated, and fluctuant lesion on her back six month prior to visiting our hospital in February 1989. The dusky red skin lesion gradually spread to the right side of her trunk and drained small amount of purulent or serosanguineous fluid. A skin biopsy specimen showed mixed pattern of nonspecific inflammatory infiltrate and granulomatous infiltrate in the dermis. A culture of the biopsy specimen showed a rapidly growing atypical acid-fast bacteria, which was identified as Mycobacterium fortuitum and classified as biovariant "third group" by positive growth on mannitol and inositol. Minimum inhibitory concentrations (MICs) of different antimicrobial agent using broth medium showed that the isolate was susceptible to the new quinolones such as ofloxacin and ciprofloxacin. The patient responded to treatment with doxycycline followed by with ofloxacin. Subspecies classification and antibiotic susceptibilities were discussed with special reference to treatment of rapidly growing mycobacteria.

Unlabelled: As is patch test using crushed live mites and crushed dried dead mites of Dermatophagoides pteronyssinus (D.p.), Dermatophagoides farinae (D.f.), & Tryophagus ptrescentiae (T.p.) was performed on 49 AD patients and 18 control patients suffering from dermatitis other than AD.

Results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 AD patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases positive to mites, (a) D.p. was positive in all 11 positive cases (100%), (b) D.f. was negative in 5 cases (0%), and (c) T.p. was positive in 2 cases among 4 (50%); 4) because of the pathological manifestations of spongiosis and the infiltration of histiocytes and lymphocytes, the possibility of contact allergy to mite elements was suggested, as a mechanism of atopic dermatitis.

Phospholipase A2 (PL-A2) has been known to be activated in either affected or un-affected skin of patients with psoriasis vulgaris. The present study was designated to investigate whether or not there is a factor activating PL-A2 in their sera, using cultured murine epidermal cells (NCTC-2544). The cultured cells prelabeled with 3H-arachidonic acid (3H-AA). After adding a serum, the release of 3H-AA into medium from the cells was measured. The sera from 20 patients with psoriasis vulgaris showed significantly increased releases of 3H-AA as compared with those by sera from 10 healthy individuals, indicating that there may be a PL-A2 activating factor in the sera of psoriasis vulgaris. The PL-A2 activating factor in the patient sera was not inactivated with added 2 different PL-A2 inhibitors. The sera were not able to produce 3H-AA from 3H-AA labeled phospholipids. Therefore, PL-A2 activating factor does not seem to be PL-A2 itself existing in the sera. Through heat stability, dialysis, and fractionation studies, the PL-A2 activating factor in sera was found to be a protein, which had a molecular weight of more than 50 kd and was stable at 56 degrees C.

Generalized pustular psoriasis in siblings, 31-year-old male and 26-year-old female, is presented. In both cases, pustular lesions appeared in childhood and typical eruption of psoriasis vulgaris was not observed during their clinical course. Cholecystitis and chronic tonsillitis of the brother and periodontitis and chronic tonsillitis of the sister were considered to be possible provocative factors. HLA-A24, Bw52-, DR2, as the common HLA haplotype in our cases, was estimated.

We reported 7 cases of drug eruption due to Iohexol (Omnipaque) which was nonionic X-ray contrast agent without severe adverse reaction. They showed generalized edematous and erythematous plaques. The eruption appeared from 5 to 6 days after the examination when a patient had not been sensitized, or several hours after that when he/she had been already sensitized. No case showed any cross-sensitization with ionic contrast media, but some cases suggested the possibility of cross-sensitization among the nonionic contrast agents.

Very Little is known about the immunological attributes of human endothelial cells. In this study, we performed immunologic phenotypic analysis of cultured human dermal microvascular endothelial cells in comparison with human umbilical vein endothelial cells and examined the ability of various biologic response modifiers to alter the phenotypes. Using FACS analysis, both types of the cells appear to lack many of the cell surface markers of immunologically proficient cells, E.G. OKT4, OKT8, Leu7, FcIgG receptor, complement receptors, IL-2 receptor and HLA-Dr, but they possess beta 2-microglobulin and DAF. HLA-Dr antigens can be induced on both types of endothelial cells by gamma-IFN in a dose and time dependent manner. Both types of endothelial cells possess several kinds of Cell Adhesion Molecules (CAMs), such as ICAM-1, CD44, LFA-3, but not LFA-1 or CD2. ICAM-1 but not LFA-3 or CD44 can be upregulated by exposure of both types of endothelial cells to gamma-IFN, IL-1 and TNF. These data suggest that endothelial cells of the dermal microvasculature may play central roles in a variety of different cutaneous inflammation.

In 1985, Carney et al reported a complex of myxomas, spotty pigmentation, and endocrine overactivity and subsequently demonstrated dominant inheritance of the condition. The criteria for diagnosis of the complex is the presence of two or more of the following conditions: (1) cardiac myxoma, (2) cutaneous myxoma, (3) mammary myxoma, (4) spotty mucocutaneous pigmentation, (5) primary pigmented nodular adrenocortical disease (Cushing's syndrome), (6) testicular tumors (sexual precocity), (7) pituitary adenoma secreting growth hormone (acromegaly or gigantism). We encountered a family with an affected mother and daughter. Case 1 was a 43-year-old woman with multiple cutaneous myxomas, mammary myxomas and spotty mucocutaneous pigmentation. Case 2, the 19-year-old daughter of case 1 had multiple cutaneous myxomas and spotty cutaneous pigmentation. These two cases both met the criteria for the diagnosis of the complex. Our report is believed to be the first report on the complex in Japan.

Serum apolipoprotein and lipid levels were determined in 33 psoriatic patients, 26 males and 7 females, and in 61 normolipemic, non-psoriatic controls matched for age and sex. The psoriatic patients had significantly higher levels of triglyceride and lower levels of apo B. The male psoriatic patients showed a tendency to have lower levels of LDL-cholesterol. The levels of cholesterol, HDL-cholesterol, apo A-I, apo A-II, apo C-II, apo C-III and apo E did not differ significantly from those of the controls. The relevance of these findings to the development of psoriasis remains to be established.