Pub Date : 2019-07-28DOI: 10.30843/NZPP.2019.72.309
M. J. Vergara, I. Pushparajah, J. Mackay, K. Everett
Phlyctema vagabunda syn. Neofabraea alba is a fungal pathogen that causes bull’s eye rot (BER) of apples. Polymerase chain reaction (PCR) primers complementary to the inter-transcribed spacer region of ribosomal DNA (ITS) and the β-tubulin gene region, and a TaqMan™ probe assay were developed to detect this pathogen. These assays were compared in quantitative PCR (qPCR) reactions for amplification of DNA extracted from several fungal species and from apple tissue. Although the ITS and the β-tubulin primers amplified all N. alba isolates, both primers also amplified a few other fungal species. The TaqMan™ probe used with published primers for N. alba only amplified N. alba isolates. The TaqMan™ assay resulted in the lowest crossing threshold (Ct) values for DNA extracted from apple fruit, leaves, and spores collected on cellophane from eight apple orchards. The TaqMan™ results were correlated with percentage BER (%BER) in a 400-apple sample harvested from the same orchards. The TaqMan™ probe assay was the most sensitive and specific qPCR protocol tested, and Ct values showed the best correlation with %BER.
{"title":"Testing new PCR primers and a TaqMan™ probe for detection of Phlyctema vagabunda syn. Neofabraea alba","authors":"M. J. Vergara, I. Pushparajah, J. Mackay, K. Everett","doi":"10.30843/NZPP.2019.72.309","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.309","url":null,"abstract":"Phlyctema vagabunda syn. Neofabraea alba is a fungal pathogen that causes bull’s eye rot (BER) of apples. Polymerase chain reaction (PCR) primers complementary to the inter-transcribed spacer region of ribosomal DNA (ITS) and the β-tubulin gene region, and a TaqMan™ probe assay were developed to detect this pathogen. These assays were compared in quantitative PCR (qPCR) reactions for amplification of DNA extracted from several fungal species and from apple tissue. Although the ITS and the β-tubulin primers amplified all N. alba isolates, both primers also amplified a few other fungal species. The TaqMan™ probe used with published primers for N. alba only amplified N. alba isolates. The TaqMan™ assay resulted in the lowest crossing threshold (Ct) values for DNA extracted from apple fruit, leaves, and spores collected on cellophane from eight apple orchards. The TaqMan™ results were correlated with percentage BER (%BER) in a 400-apple sample harvested from the same orchards. The TaqMan™ probe assay was the most sensitive and specific qPCR protocol tested, and Ct values showed the best correlation with %BER.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86357224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-28DOI: 10.30843/NZPP.2019.72.325
Bingqin Xu, D. Teulon
References to many other kiwifruit arthropod pests (and diseases) were found as part of a process of searching Chinese language literature to understand the impact of brown marmorated stink bug (Halyomorpha halys; BMSB) and spotted lantern fly (Lycorma delicatula; SLF) on kiwifruit (Actinidia chinensis) and A. deliciosa in China. Information on other kiwifruit pests was collated from over 20 Chinese language publications found in searches of the Chinese National Knowledge Infrastructure platform using standard Chinese characters for BMSB, SLF and kiwifruit, and was ranked according to: (1) the number of publications they were mentioned in; and (2) the type of words used to describe their impact. In addition to BMSB and SLF, approximately 50 kiwifruit pests were identified from this process, including a number of species that were unknown to the New Zealand kiwifruit biosecurity community and which may pose a threat to kiwifruit production if they established in New Zealand. This work reinforces the need for searching Chinese databases with Chinese characters in combination with searches in international databases, to ensure comprehensive coverage for biosecurity risk assessment.
{"title":"Arthropod pests of kiwifruit identified from Chinese language literature","authors":"Bingqin Xu, D. Teulon","doi":"10.30843/NZPP.2019.72.325","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.325","url":null,"abstract":"References to many other kiwifruit arthropod pests (and diseases) were found as part of a process of searching Chinese language literature to understand the impact of brown marmorated stink bug (Halyomorpha halys; BMSB) and spotted lantern fly (Lycorma delicatula; SLF) on kiwifruit (Actinidia chinensis) and A. deliciosa in China. Information on other kiwifruit pests was collated from over 20 Chinese language publications found in searches of the Chinese National Knowledge Infrastructure platform using standard Chinese characters for BMSB, SLF and kiwifruit, and was ranked according to: (1) the number of publications they were mentioned in; and (2) the type of words used to describe their impact. In addition to BMSB and SLF, approximately 50 kiwifruit pests were identified from this process, including a number of species that were unknown to the New Zealand kiwifruit biosecurity community and which may pose a threat to kiwifruit production if they established in New Zealand. This work reinforces the need for searching Chinese databases with Chinese characters in combination with searches in international databases, to ensure comprehensive coverage for biosecurity risk assessment.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90985559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-28DOI: 10.30843/NZPP.2019.72.6980
L. Hasna, K. Everett, M. J. Vergara, I. Pushparajah, P. Wood, B. M. Fisher, Lucia R. Ramos, C. Middleditch, S. Olsson, A. Nangul, Jung Ook Cho, A. Woolf
Bull’s eye rot (BER) of apples is caused by a postharvest fungal pathogen (Phlyctema vagabunda syn. Neofabraea alba). Previous laboratory experiments found hot water treatments (HWT) resulted in a significant reduction of BER incidence for artificially inoculated fruit so the feasibility of HWT to control naturally infected fruit in a semi-commercial trial was tested. One bin (1934 fruit) of naturally infected ‘Scired’ apples was harvested from a Hawke’s Bay orchard with a known high incidence of BER, then placed in a coolstore for 1 week until treated. All fruit were passed through a high-pressure water blaster then air dried. Approximately half the contents of the bin (1034 fruit) were packed into Friday trays in apple boxes with a plastic polyliner. The other half (900 fruit) were treated for 2 min with hot water at 51°C in a semi-commercial hot water bath before packing. All fruit were then coolstored for 20 weeks before assessment for BER. This HWT resulted in a 6-fold reduction of BER incidence so was an effective treatment for BER in a semi-commercial test.
苹果牛眼腐病(BER)是由一种采后真菌病原菌引起的。先前的实验室实验发现，热水处理(HWT)可以显著降低人工接种水果的BER发病率，因此在半商业化试验中测试了热水处理控制自然感染水果的可行性。从已知BER高发的Hawke ' s Bay果园收获一箱(1934个水果)自然感染的' Scired '苹果，然后放置在冷藏库中1周直至处理。所有的水果都经过高压水喷射器，然后风干。大约一半的水果(1034个水果)被包装在星期五的托盘里，用塑料聚氯乙烯包装苹果盒。另一半(900个水果)在包装前在半商用热水浴中用51°C的热水处理2分钟。所有水果冷藏20周后进行BER评估。该HWT使BER发病率降低了6倍，因此在半商业化试验中是BER的有效治疗方法。
{"title":"Semi-commercial hot water treatments for control of bull’s eye rot of apples","authors":"L. Hasna, K. Everett, M. J. Vergara, I. Pushparajah, P. Wood, B. M. Fisher, Lucia R. Ramos, C. Middleditch, S. Olsson, A. Nangul, Jung Ook Cho, A. Woolf","doi":"10.30843/NZPP.2019.72.6980","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.6980","url":null,"abstract":"Bull’s eye rot (BER) of apples is caused by a postharvest fungal pathogen (Phlyctema vagabunda syn. Neofabraea alba). Previous laboratory experiments found hot water treatments (HWT) resulted in a significant reduction of BER incidence for artificially inoculated fruit so the feasibility of HWT to control naturally infected fruit in a semi-commercial trial was tested. One bin (1934 fruit) of naturally infected ‘Scired’ apples was harvested from a Hawke’s Bay orchard with a known high incidence of BER, then placed in a coolstore for 1 week until treated. All fruit were passed through a high-pressure water blaster then air dried. Approximately half the contents of the bin (1034 fruit) were packed into Friday trays in apple boxes with a plastic polyliner. The other half (900 fruit) were treated for 2 min with hot water at 51°C in a semi-commercial hot water bath before packing. All fruit were then coolstored for 20 weeks before assessment for BER. This HWT resulted in a 6-fold reduction of BER incidence so was an effective treatment for BER in a semi-commercial test.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80910131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-28DOI: 10.30843/NZPP.2019.72.322
M. Toome‐Heller, B. Alexander
During the inspection of imported sweet corn (Zea mays), a specimen with dry rot symptoms was detected by Ministry for Primary Industries quarantine officers. A sample was sent to the MPI Plant Health and Environment Laboratory for diagnostics, and initial examination found a layer of yeast cells on the surface of the symptomatic tissue. The fungus was cultured and identified based on DNA sequences as Ustilago maydis. While the corn-smut pathogen U. maydis is well known to cause tumour like galls on corn kernels, it is a less recognised fact that this fungus can also grow as a yeast. To determine if the yeast stage could have been associated with the dry-rot symptoms observed on the specimen, healthy material was inoculated with the isolated U. maydis strain. No symptoms developed on inoculated material, indicating that the yeast cells were likely multiplying on the surface of the cut corn ear as saprobes. To our knowledge, this is the first report of U. maydis yeast stage on corn ears and indicates a previously unconsidered pathway for the organism. For the yeast stage to cause disease, mating with a compatible mating type on the surface of a living host plant would be required.
{"title":"Ustilago maydis yeast stage found on imported sweet corn","authors":"M. Toome‐Heller, B. Alexander","doi":"10.30843/NZPP.2019.72.322","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.322","url":null,"abstract":"During the inspection of imported sweet corn (Zea mays), a specimen with dry rot symptoms was detected by Ministry for Primary Industries quarantine officers. A sample was sent to the MPI Plant Health and Environment Laboratory for diagnostics, and initial examination found a layer of yeast cells on the surface of the symptomatic tissue. The fungus was cultured and identified based on DNA sequences as Ustilago maydis. While the corn-smut pathogen U. maydis is well known to cause tumour like galls on corn kernels, it is a less recognised fact that this fungus can also grow as a yeast. To determine if the yeast stage could have been associated with the dry-rot symptoms observed on the specimen, healthy material was inoculated with the isolated U. maydis strain. No symptoms developed on inoculated material, indicating that the yeast cells were likely multiplying on the surface of the cut corn ear as saprobes. To our knowledge, this is the first report of U. maydis yeast stage on corn ears and indicates a previously unconsidered pathway for the organism. For the yeast stage to cause disease, mating with a compatible mating type on the surface of a living host plant would be required.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88497359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.257
M. Kuchár, T. Glare, J. Hampton, I. Dickie, M. Christey
Plutella xylostella (diamondback moth) is a prominent pest of brassicas which is now resistant to most insecticides. Despite years of research, the range of available products used in biological control of diamondback moth is still somewhat limited. We isolated putative endophytic fungi from New Zealand cabbage plants to search for unique biological control agents of diamondback moth larvae. The larvae were fed leaf discs from commercially grown cabbage covered in spores from endophytic fungal isolates to test the insecticidal properties of these fungi. Twenty of the 52 fungal isolates tested failed to kill any diamondback moth larvae. However, three isolates of Lecanicillium muscarium induced mortality greater than 80%. While these isolates have potential for use in biological control applications, further research into propagation, formulation, and method, rate and timing of application is needed.
{"title":"Virulence of the plant-associated endophytic fungus Lecanicillium muscarium to diamondback moth larvae","authors":"M. Kuchár, T. Glare, J. Hampton, I. Dickie, M. Christey","doi":"10.30843/NZPP.2019.72.257","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.257","url":null,"abstract":"Plutella xylostella (diamondback moth) is a prominent pest of brassicas which is now resistant to most insecticides. Despite years of research, the range of available products used in biological control of diamondback moth is still somewhat limited. We isolated putative endophytic fungi from New Zealand cabbage plants to search for unique biological control agents of diamondback moth larvae. The larvae were fed leaf discs from commercially grown cabbage covered in spores from endophytic fungal isolates to test the insecticidal properties of these fungi. Twenty of the 52 fungal isolates tested failed to kill any diamondback moth larvae. However, three isolates of Lecanicillium muscarium induced mortality greater than 80%. While these isolates have potential for use in biological control applications, further research into propagation, formulation, and method, rate and timing of application is needed.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83829428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.285
M. Trolove, P. Shorten
Rapid advancements in UAVs, computing power and photogrammetry techniques now permit low cost biological-monitoring applications using off-the-shelf hardware and software. The utility of four UAV models costing $1,200 - $11, 000 and two photogrammetry programmes were assessed in separate experiments to evaluate their ability to detect standardised plant targets and to generate useable orthomoasic images. The colour and contrast of standardised targets influenced detection by UAVs more than their size as height increased. A large green rosette (50.8 cm2) could be detected by all UAVs from 28–90 m, while a yellow target 13 times smaller could be detected at 36–100 m, with the more expensive UAVs being effective at the higher altitudes. Monitoring vegetation cover or flowering plants is possible at the minimum allowable height altitude of 20 m by all four UAVs. However, identification of species in their vegetative state would require the UAVs with the better camera optics. The two photogrammetry programmes produced suitable orthomosaic images under the pasture, maize and hill country scenarios tested.
{"title":"Comparison of four off-the-shelf unmanned aerial vehicles (UAVs) and two photogrammetry programmes for monitoring pasture and cropping field trials","authors":"M. Trolove, P. Shorten","doi":"10.30843/NZPP.2019.72.285","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.285","url":null,"abstract":"Rapid advancements in UAVs, computing power and photogrammetry techniques now permit low cost biological-monitoring applications using off-the-shelf hardware and software. The utility of four UAV models costing $1,200 - $11, 000 and two photogrammetry programmes were assessed in separate experiments to evaluate their ability to detect standardised plant targets and to generate useable orthomoasic images. The colour and contrast of standardised targets influenced detection by UAVs more than their size as height increased. A large green rosette (50.8 cm2) could be detected by all UAVs from 28–90 m, while a yellow target 13 times smaller could be detected at 36–100 m, with the more expensive UAVs being effective at the higher altitudes. Monitoring vegetation cover or flowering plants is possible at the minimum allowable height altitude of 20 m by all four UAVs. However, identification of species in their vegetative state would require the UAVs with the better camera optics. The two photogrammetry programmes produced suitable orthomosaic images under the pasture, maize and hill country scenarios tested.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82028908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.301
P. Lo, R. Wallis, D. Bellamy
Sticky traps for monitoring insects use polybutene adhesive (PBA) to entangle insects. This glue is effective but messy to use and an alternative, hot-melt pressure-sensitive adhesive (HMPSA) is available. The effectiveness of these two adhesives was compared for catching pest and beneficial insects, primarily in apples. Various types of trap with either PBA or HMSPA were placed in orchards and vineyards in Hawke’s Bay and Nelson. Eight pests from six families, six parasitoids, five predators and one pollinator were commonly recorded. Traps with HMPSA generally caught similar numbers or more of both insect pests and beneficial insects than traps with PBA. Traps with HMPSA performed better for larger insects (>1.5 mm), whereas those with PBA tended to be more effective for smaller insects. Both types of adhesive were effective for up to 4 weeks. HMPSA was effective for monitoring a range of insect pests and beneficial insects. Compared with PBA, HMPSA was more consistent and much cleaner and easier to use. It is recommended that HMPSA replaces PBA in traps for monitoring moth pests in the pipfruit industry.
{"title":"The effectiveness of two types of adhesive for catching insects in traps","authors":"P. Lo, R. Wallis, D. Bellamy","doi":"10.30843/NZPP.2019.72.301","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.301","url":null,"abstract":"Sticky traps for monitoring insects use polybutene adhesive (PBA) to entangle insects. This glue is effective but messy to use and an alternative, hot-melt pressure-sensitive adhesive (HMPSA) is available. The effectiveness of these two adhesives was compared for catching pest and beneficial insects, primarily in apples. Various types of trap with either PBA or HMSPA were placed in orchards and vineyards in Hawke’s Bay and Nelson. Eight pests from six families, six parasitoids, five predators and one pollinator were commonly recorded. Traps with HMPSA generally caught similar numbers or more of both insect pests and beneficial insects than traps with PBA. Traps with HMPSA performed better for larger insects (>1.5 mm), whereas those with PBA tended to be more effective for smaller insects. Both types of adhesive were effective for up to 4 weeks. HMPSA was effective for monitoring a range of insect pests and beneficial insects. Compared with PBA, HMPSA was more consistent and much cleaner and easier to use. It is recommended that HMPSA replaces PBA in traps for monitoring moth pests in the pipfruit industry.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79426021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.305
D. Iorio, M. Walter, E. Lantinga, H. Kerckhoffs, R. Campbell
European canker (EC), caused by Neonectria ditissima, is an important disease in apple-producing regions in New Zealand. In order to improve plant protection, Geographic Information Systems (GIS) can be used to map plant disease location and severity in agricultural settings. Data were compiled from apple growers in Tasman, New Zealand, to investigate EC distribution over 4 years, for the period 2015–2018. ArcGIS software, including the Spatial Analyst, Interpolation and Geospatial statistics toolboxes, was used to map EC incidence at the spatial scale of orchard blocks, which allowed the identification of disease hot-spots. A clustered spatial pattern of disease was detected every year and areas with higher risk of EC were identified within the region. The spatial patterns detected were related to disease pressure over time for different apple cultivars. The use of GIS provides a platform for analysing and visually communicating disease patterns over time. Investigating disease spatial pattern allows the inference of spatial processes and further hypothesis generation to understand the pathogen.
{"title":"Mapping European canker spatial pattern and disease progression in apples using GIS, Tasman, New Zealand","authors":"D. Iorio, M. Walter, E. Lantinga, H. Kerckhoffs, R. Campbell","doi":"10.30843/NZPP.2019.72.305","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.305","url":null,"abstract":"European canker (EC), caused by Neonectria ditissima, is an important disease in apple-producing regions in New Zealand. In order to improve plant protection, Geographic Information Systems (GIS) can be used to map plant disease location and severity in agricultural settings. Data were compiled from apple growers in Tasman, New Zealand, to investigate EC distribution over 4 years, for the period 2015–2018. ArcGIS software, including the Spatial Analyst, Interpolation and Geospatial statistics toolboxes, was used to map EC incidence at the spatial scale of orchard blocks, which allowed the identification of disease hot-spots. A clustered spatial pattern of disease was detected every year and areas with higher risk of EC were identified within the region. The spatial patterns detected were related to disease pressure over time for different apple cultivars. The use of GIS provides a platform for analysing and visually communicating disease patterns over time. Investigating disease spatial pattern allows the inference of spatial processes and further hypothesis generation to understand the pathogen.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82456792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.249
M. Kanjanamaneesathian, P. Meetum
Bacillus megaterium is a beneficial bacterium that is used as a biological control agent (BCA) against the fungi Rhizoctonia solani, Fusarium sacchari and Curvularia lunata, which attack rice plants. However, the cost of preparing the bacterium using standard nutrient broth is prohibitively expensive on a large scale. Therefore, a low-cost product (seasoning cube, a common ingredient for cooking) was examined as an alternative nutrient medium. Bacillus megaterium was cultured in either nutrient broth or in dissolved seasoning cube. These cultures were evaluated for their effect on the growth of rice seedlings in the laboratory and to suppress grain discoloration of rice in small-scale field trials. Bacillus megaterium cultured with a seasoning cube was as effective as standard nutrient broth for the growth of rice seedlings in the laboratory. It also suppressed grain discoloration disease of rice in small-scale field trials. Use of a seasoning cube is suitable for culturing B. megaterium and should be recommended to farmers.
巨芽孢杆菌是一种有益的细菌，被用作生物防治剂(BCA)，以防止真菌solani Rhizoctonia, sacchari Fusarium and Curvularia lunata侵袭水稻植株。然而，大规模使用标准营养肉汤制备细菌的成本高得令人望而却步。因此，研究了一种低成本的产品(调味块，一种常见的烹饪原料)作为替代营养培养基。在营养液和调味块中培养巨型芽孢杆菌。在实验室中评估了这些培养物对水稻幼苗生长的影响，并在小规模田间试验中评估了它们对水稻籽粒变色的抑制作用。用调味块培养的巨芽孢杆菌在实验室中与标准营养液培养水稻幼苗一样有效。在小规模的田间试验中，它还能抑制水稻的籽粒变色病。使用调味剂适合栽培巨型白刺鸡，应推荐给农民。
{"title":"Comparison of aqueous seasoning cube solution and nutrient broth as culture media for production of the biocontrol agent Bacillus megaterium in the laboratory, and for suppression of rice grain discolouration in the field","authors":"M. Kanjanamaneesathian, P. Meetum","doi":"10.30843/NZPP.2019.72.249","DOIUrl":"https://doi.org/10.30843/NZPP.2019.72.249","url":null,"abstract":"Bacillus megaterium is a beneficial bacterium that is used as a biological control agent (BCA) against the fungi Rhizoctonia solani, Fusarium sacchari and Curvularia lunata, which attack rice plants. However, the cost of preparing the bacterium using standard nutrient broth is prohibitively expensive on a large scale. Therefore, a low-cost product (seasoning cube, a common ingredient for cooking) was examined as an alternative nutrient medium. Bacillus megaterium was cultured in either nutrient broth or in dissolved seasoning cube. These cultures were evaluated for their effect on the growth of rice seedlings in the laboratory and to suppress grain discoloration of rice in small-scale field trials. Bacillus megaterium cultured with a seasoning cube was as effective as standard nutrient broth for the growth of rice seedlings in the laboratory. It also suppressed grain discoloration disease of rice in small-scale field trials. Use of a seasoning cube is suitable for culturing B. megaterium and should be recommended to farmers.","PeriodicalId":19180,"journal":{"name":"New Zealand Plant Protection","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85552745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-27DOI: 10.30843/NZPP.2019.72.304
B. Howlett, Megan Gee
The drone fly (Eristalis tenax) pollinates many crops and is found almost worldwide. Its successful management as a field-crop pollinator would provide an additional option to augment bee pollination. We reviewed literature to assess their management potential. A literature search was conducted for information on drone-fly abundance across New Zealand crops, pollinator effectiveness, lifecycle-requirements and potential for mass rearing. Relevant literature was then evaluated to assess the feasibility, benefits and limitations of their management. Eristalis tenax is a proven pollinator of pak choi (Brassica rapa spp. chinensis), kiwifruit (Actinidia deliciosa) and onion (Allium cepa), and visits the flowers of several more crops in New Zealand. It readily completes its lifecycle under laboratory conditions when reared on various organic materials. No reviewed studies were identified that showed successful management of populations for the purpose of field-crop pollination. Key challenges for their management as field-crop pollinators include: being able to mass rear them at an appropriate scale; retaining numbers within targeted areas in the field; and ensuring their use does not significantly impact on non-target species and land-user interests.
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