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Effect of Fermentation Time on the Phenolic, Flavonoid and Vitamin C Contents and Antioxidant Activities of Okra (Abelmoschus esculentus) Seeds 发酵时间对秋葵种子酚类、黄酮类和维生素C含量及抗氧化活性的影响
Pub Date : 2014-01-01 DOI: 10.1016/S0189-7241(15)30128-4
Adetuyi F.O. , Ibrahim T.A.

The objective of this study was to evaluate the effect of fermentation on the phenolic, vitamin C and total flavonoid contents and antioxidant properties of okra seed. The okra seeds were removed from the pod of matured and fibrous okra that cannot be easily cut with kitchen knife, and was allowed to undergo chance fermentation for 120 h. Samples were taken for antioxidant analysis at 24 h, 72 h and 120 h, respectively. The aqueous extracts of fermented and unfermented okra seeds were obtained and subsequently used for the analysis. The phenolic, vitamin C and total flavonoid contents and the antioxidant properties (ferric reducing antioxidant power (FRAP) and 1, 1-diphenyl-2 picrylhydrazyl (DPPH) free radical-scavenging ability) of the extracts were determined. The results revealed that fermented okra seeds had significantly (p < 0.05) higher phenolic content, vitamin C, total flavonoid and non-flavonoid contents and showed greater antioxidant activities than unfermented okra seed. Okra seeds fermented for 24 h exhibited the highest ferric reducing antioxidant power of 980 mgAAE/100 g and the least IC50 of 2.27 mg/ ml for DPPH free radical-scavenging ability than the others. Okra seeds fermented for 24 hours had significantly (p < 0.05) higher antioxidant activities. The product from fermented okra seeds could be used for the production of functional foods.

本研究旨在探讨发酵对秋葵种子酚类、维生素C、总黄酮含量及抗氧化性能的影响。从不易用菜刀切开的成熟秋葵和纤维性秋葵豆荚中取出秋葵种子,进行随机发酵120 h,分别在24 h、72 h和120 h采集样品进行抗氧化分析。获得发酵和未发酵秋葵种子的水提取物,随后用于分析。测定了提取物的酚类、维生素C和总黄酮含量以及抗氧化性能(铁还原抗氧化能力(FRAP)和清除1,1 -二苯基-2苦酰肼(DPPH)自由基的能力)。结果表明,发酵秋葵种子具有显著的(p <黄酮类化合物含量、维生素C、总黄酮和非黄酮类化合物含量均高于未发酵的秋葵种子,抗氧化活性也高于未发酵的秋葵种子。发酵24 h后,秋葵种子的铁还原能力最高,为980 mgAAE/100 g,清除DPPH自由基的IC50最低,为2.27 mg/ ml。发酵24小时后,秋葵种子显著(p <0.05)抗氧化活性较高。秋葵种子发酵后的产物可用于功能性食品的生产。
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引用次数: 115
Persistence and Biofilm Assessment of Campylobacter Jujeni in Poultry Abattoir 聚氏弯曲杆菌在家禽屠宰场的持久性及生物膜评价
Pub Date : 2014-01-01 DOI: 10.1016/S0189-7241(15)30096-5
Balogu T.V. , Nwaugo V.O. , Onyeagba R.A.

Persistence of Campylobacter sp and its biofilm forming ability was assessed in two poultry abattoirs at two weeks intervals. Average prevalence (63.75%) of Campylobacter spp. was observed on assessing a total of 160 samples collected from the surfaces of packaging table (80%), dressing table (75%), floor source (70%) and washing table (30%). Biofilm assessment formed by Campylobacter jejuni within 5-days at 37°C were in decreasing order of washing table > packaging table > dressing table > floor. An average rate (19.6%) of isolates to develop biofilm observed in both sites was considered relatively low. Absorbance value (Optical Density-OD590nm) of formed biofilms ranged from 0.483 – 0.952. Wastewater from the facilities showed higher TDS (643 – 820 mgl−1), TSS (1200 – 1775 mgl−1), COD (152 – 141 mgl−1) and BOD (30.3 – 32.5mgl−1) than the WHO standards of 500 mgl−1, 100 mgl−1, 10 mgl−1 and 6 mgl−1 respectively. This is a clear indication of heavy microbial presence in the wastewater. Total bacterial count (TBC) was slightly higher in site A (4.4 × 105 CFU/ml) than site B (3.5 × 105 CFU/ml). Efficiency index ratio (≈/> 1) observed in all tested drugs suggests their effectiveness in campylobacteriosis management. Decreasing drug sensitivity pedigree was observed with streptomycin > erythromycin & gentamincin > tetracycline & neomycin > penicillin > riphapicin & ampicillin > norflaxicin & cephalexin. These results of frequency and biofilm forming tendencies of Campylobacter spp. observed in this study can be of value in checkmating campybacteriosis outbreak from poultry abattoir facility.

在两个家禽屠宰场每隔两周评估弯曲杆菌sp的持久性及其生物膜形成能力。对包装台(80%)、梳妆台(75%)、地板源(70%)和洗涤台(30%)表面采集的160份样品进行检测,发现弯曲杆菌平均检出率为63.75%。空肠弯曲菌在37℃条件下5天内形成的生物膜评价依次递减;包装表>梳妆台>地板上。在两个地点观察到的分离株形成生物膜的平均率(19.6%)被认为是相对较低的。形成的生物膜吸光度值(光密度- od590nm)在0.483 ~ 0.952之间。废水TDS (643 ~ 820 mg - 1)、TSS (1200 ~ 1775 mg - 1)、COD (152 ~ 141 mg - 1)和BOD (30.3 ~ 32.5mg - 1)分别高于WHO标准500 mg - 1、100 mg - 1、10 mg - 1和6 mg - 1。这清楚地表明废水中存在大量微生物。细菌总数(TBC) A区(4.4 × 105 CFU/ml)略高于B区(3.5 × 105 CFU/ml)。效率指数比(≈/>1)所有被试药物的观察结果表明其对弯曲杆菌病的治疗有效。链霉素的药敏谱系降低;红霉素,gentamincin祝辞四环素和新霉素祝辞青霉素比;riphapicin,氨苄青霉素比;norflaxicin,头孢氨苄。本研究所观察到的弯曲杆菌的频率和生物膜形成趋势对控制家禽屠宰场弯曲杆菌病的爆发具有一定的价值。
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引用次数: 4
Evaluation of Water-soluble Vitamins and Optimum Cooking Time of Fresh Edible Portions of Elephant Grass (Pennisetum purpureum L. Schumach) Shoot 象草(Pennisetum purpureum L. Schumach)嫩枝鲜食部分水溶性维生素及最佳蒸煮时间的评价
Pub Date : 2014-01-01 DOI: 10.1016/S0189-7241(15)30127-2
Akah N.P. , Onweluzo J.C.

Fresh edible inner tender stem (matrix) and base of domesticated elephant grass shoot were evaluated for water-soluble vitamins B1, B2, B3, B6 and vitamin C. The B-vitamins were determined using high performance liquid chromatographic (HPLC) technique, while vitamin C was evaluated using 2, 6 dichlorophenolindophenol (DCIP) titration method. The optimum blanching time of the matrix was also determined. Samples were water-blanched from 0 to 16 min at intervals of 2 min. Optimum cooking time was determined to be the time at which vitamin C showed maximum retention after the intervals of blanching. Vitamins B1, B2, B3, B6 and C concentrations (mg/100 g fresh weight) in the samples were respectively 1.23 mg/100 g, 4.37 mg/100 g, 12.35 mg/100 g, 2.40 mg/100 g and 17.23 mg/100 g in the matrix and 0.86 mg/100 g, 3.72 mg/100 g, 6.40 mg/100 g, 1.59 mg/100 g and 18.30 mg/100 g in the base. The amounts of the B-vitamins in 100 g portion of the samples adequately satisfy the RDAs for the respective B-vitamins for normal healthy adults. The matrix was significantly higher (p < 0.05) than the base in vitamins B1, B3 and B6. The matrix showed an optimum blanching time of 10 min. This was considered the optimum cooking time required to avoid significant loss of vitamin C and other heat-labile nutrients in elephant grass matrix.

采用高效液相色谱(HPLC)法测定新鲜食用象草内嫩茎(基质)和栽培象草茎底的水溶性维生素B1、B2、B3、B6和维生素C含量,采用2,6二氯酚吲哚酚(DCIP)滴定法测定维生素C含量。确定了基质的最佳漂烫时间。以2分钟的间隔对样品进行水焯,时间为0 ~ 16分钟。确定最佳蒸煮时间为焯水间隔后维生素C保留量最大的时间。样品中维生素B1、B2、B3、B6和C的浓度(mg/100 g鲜重)在基质中分别为1.23 mg/100 g、4.37 mg/100 g、12.35 mg/100 g、2.40 mg/100 g和17.23 mg/100 g,在基质中分别为0.86 mg/100 g、3.72 mg/100 g、6.40 mg/100 g、1.59 mg/100 g和18.30 mg/100 g。100g部分样品中b族维生素的含量完全满足正常健康成人相应b族维生素的rda要求。基质显著增高(p <0.05),高于维生素B1、B3和B6的基础含量。该基质的最佳焯水时间为10 min。这被认为是避免象草基质中维生素C和其他热不稳定营养物质大量流失的最佳蒸煮时间。
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引用次数: 20
Effects of Processing on the Nutrient Composition of False Yam (Icacina trichantha) Flour 加工对假山药面粉营养成分的影响
Pub Date : 2014-01-01 DOI: 10.1016/S0189-7241(15)30111-9
Umoh E.O. , Iwe M.O.

False yam (Icacina trichantha) tubers were processed into different flour samples: the raw; steeped-sun-dried; steeped- oven-dried; blanched-sun-dried and blanched-oven-dried samples. The nutrient composition: proximate and mineral elements contents of the flour samples were studied. The lipid, protein and carbohydrate contents of the samples ranged between 1.77 to 5.76%, 3.01 to 60.3% and 85.56 to 93.31% respectively, while the energy values ranged between 394.27 to 412.46 Kcal. Concentration of potassium, sodium, calcium and iron (among others) ranged between 20.835 and 31.51 mg/l, 2.409 and 18.890 mg/l, 90.250 and 112.550 mg/l to 0.777 and 2.840 mg/l respectively. False yam tuber, apart from being a good source of energy for man, is rich in mineral elements (potassium, sodium and calcium).

Processing affected the levels of nutrients, in the flour samples.

假山药(Icacina trichantha)块茎被加工成不同的面粉样品:原料;steeped-sun-dried;浸泡,烘干的;热晒和热烘干样品。研究了面粉样品的营养成分:近似值和矿质元素含量。样品的脂肪、蛋白质和碳水化合物含量分别在1.77 ~ 5.76%、3.01 ~ 60.3%和85.56 ~ 93.31%之间,能量值在394.27 ~ 412.46 Kcal之间,钾、钠、钙、铁等的浓度分别在20.835 ~ 31.51 mg/l、2.409 ~ 18.890 mg/l、90.250 ~ 112.550 mg/l ~ 0.777和2.840 mg/l之间。假山药块茎,除了是一个很好的能量来源,是丰富的矿物元素(钾,钠和钙)。加工过程影响了面粉样品中的营养成分。
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引用次数: 15
Shelf Life Extension of Toasted Groundnuts through the Application of Cassava Starch and Soy Protein-Based Edible Coating 木薯淀粉和大豆蛋白基食用涂层对烤花生保质期的影响
Pub Date : 2014-01-01 DOI: 10.1016/S0189-7241(15)30106-5
Chinma C.E. , Ariahu C.C. , Abu J.O.

The use of cassava starch and soy protein concentrate edible coatings containing 20% glycerol in extending the shelf life of toasted groundnut during ambient (27 ± 1°C) storage for 14 days was studied. Chemical indices of oxidative rancidity and sensory parameters were evaluated using standard procedures. Moisture uptake, peroxide and thiobarbituric acid values of uncoated groundnuts were higher than 100% cassava starch coated groundnuts while toasted groundnuts coated with 50:50 (cassava starch:soy protein concentrate) had the lowest values. Toasted groundnuts coated with 50:50 (Cassava starch:soy protein concentrate) film had higher colour, taste, texture and overall acceptability scores than toasted groundnuts coated with 100% cassava starch film and control. The use of 50:50 (cassava starch:soy protein concentrate) edible coatings on toasted groundnut extended the shelf life of toasted groundnuts for 14 days compared to uncoated toasted groundnuts which developed objectionable taste after second day of storage at ambient (27 ± 1°C) condition.

研究了木薯淀粉和含20%甘油的浓缩大豆蛋白可食性包衣对花生在环境(27±1℃)贮藏14 d的延长效果。采用标准程序评价氧化酸败的化学指标和感官参数。未包被花生的吸湿、过氧化物和硫代巴比妥酸值高于100%木薯淀粉包被花生,而50:50(木薯淀粉:大豆浓缩蛋白)包被花生的烤花生的吸湿、过氧化物和硫代巴比妥酸值最低。涂有50:50(木薯淀粉:大豆蛋白浓缩物)薄膜的烤花生比涂有100%木薯淀粉薄膜和对照的烤花生具有更高的颜色、味道、质地和总体可接受性得分。使用50:50(木薯淀粉:大豆蛋白浓缩物)的可食用涂层,烤花生的保质期延长了14天,而未涂涂层的烤花生在环境(27±1℃)条件下储存第2天后出现难闻的味道。
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引用次数: 9
Physicochemical Properties and Phytochemical Components of Spiced Cucumber-Pineapple Fruit Drink 五香黄瓜菠萝果饮料的理化性质及植物化学成分研究
Pub Date : 2013-01-01 DOI: 10.1016/S0189-7241(15)30055-2
Babajide J.M. , Olaluwoye A.A. , Taofik Shittu T.A. , Adebisi M.A.

A blend of equal portions of cucumber (50%) and pineapple (50%) juices was mixed with clove and ginger powder spices at 0.25% (CPCLG1), 0.5 (CPCLG2), 0.75% (CPCLG3) and 1% (CPCLG4) (w/v) respectively to develop a new fruit drink with health benefits. The juice blend without spice extract (CP) was used as reference sample. Samples were analysed for oBrix, pH, acidity, specific gravity, total solids and ascorbic acid (vitamin C). The phytochemicals present in the products were also determined using chemical method and Gas chromatography-mass spectrometry (GC-MS). There was significant reduction in the oBrix from 8.08 to 7.60%, pH (4.41 to 4.36) and total solid (7.96 to 7.71%). Specific gravity was 1.020 while the acidity increased from 0.20 to 0.22% as the levels of spices increased. The phytochemical compounds identified in the products include alkaloids, flavonoids, saponins, steroids, tannins, terpenoids and phlobatannins. The quantitative phytochemical contents showed 0.1 μg/ml Isoquinoline in CP, 0.03271 μg/ml Isoquinoline in CPCLG1; 0.00199 μg/ml Octanoid acid, 0.00224 μg/ml Metroprolol, 0.00231 μg/ml Fumaric acid, 0.00263 μg/ml Benzoquinone, 0.00264 μg/ml Betaxolol, and 0.002471 μg/ml 1-nonene in CPCLG2; 0.00070 μg/ml Limonene and 0.00146 μg/ml Caryophyllene in CPCLG3 and 0.00048 μg/ml P-Benzoquinone, 0.00074 μg/ml 4H-Quinolizine, 0.00017 μg/ml 3-pyridinepropanol, 0.00559 μg/ml Chlorogenic acid, 0.00559 μg/ml Camphene and 0.00089 μg/ml Benzofuranone in CPCLG4. The types and quantities of phytochemicals present in the products were influenced by the formulation of the products.

将等量黄瓜汁(50%)和菠萝汁(50%)分别与0.25% (CPCLG1)、0.5 (CPCLG2)、0.75% (CPCLG3)和1% (CPCLG4) (w/v)的丁香和姜粉香料混合,研制出一种具有保健功效的新型果汁饮料。以不加香料提取物(CP)的混合果汁为参比样品。分析样品的oBrix、pH、酸度、比重、总固形物和抗坏血酸(维生素C),并使用化学方法和气相色谱-质谱(GC-MS)测定产品中的植物化学物质。基质从8.08降至7.60%,pH从4.41降至4.36,总固形物从7.96降至7.71%。比重为1.020,酸度随香料添加量的增加从0.20增加到0.22%。在产品中鉴定的植物化学化合物包括生物碱、类黄酮、皂苷、类固醇、单宁、萜类和酞菁。CP中异喹啉含量为0.1 μg/ml, CPCLG1中异喹啉含量为0.03271 μg/ml;CPCLG2中辛烷酸0.00199 μg/ml、美特洛尔0.00224 μg/ml、富马酸0.00231 μg/ml、苯醌0.00263 μg/ml、倍他洛尔0.00264 μg/ml、1-壬烯0.002471 μg/ml;CPCLG3中柠檬烯含量为0.00070 μg/ml、石竹烯含量为0.00146 μg/ml,对苯醌含量为0.00048 μg/ml、4h -喹啉含量为0.00074 μg/ml、3-吡啶丙醇含量为0.00017 μg/ml、绿原酸含量为0.00559 μg/ml、莰烯含量为0.00559 μg/ml、苯并呋喃酮含量为0.00089 μg/ml。产品中存在的植物化学物质的类型和数量受到产品配方的影响。
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引用次数: 25
Physicochemical Properties of Mango Seed Flour 芒果籽粉的理化性质
Pub Date : 2013-01-01 DOI: 10.1016/S0189-7241(15)30052-7
Okpala L.C., Gibson-Umeh G.I.

The proximate composition, functional and antinutritional properties of flour produced from seeds of mangoes grown in Ebonyi State were studied. Two cultivars: India and Indochinese were used for the study. The aim of the study was to determine any possible potential that flour obtained from these seeds might possess. Results revealed that flours from India and Indochinese cultivars had protein contents of 6.00 and 4.95%; moisture, 5.00 and 10.36%; fibre, 11.00 and 15% while the carbohydrate was 74.41 and 64.23% respectively. Results from the functional properties showed that flours from both of the cultivars had water absorption capacities of 2.0 g/g and 1.5 g/g; oil absorption capacity of 2.16 g/g and 1.83 g/g while foaming capacity was 3.79 g/g and 3.75 g/g with India cultivar having higher values for each of the functional properties studied although, there was no significant difference (p > 0.05) in the values except for the water absorption capacity. Levels of hydrogen cyanide, phytic acid and trypsin inhibitors were generally low and within safe levels.

研究了以乌邦伊州芒果种子为原料生产的面粉的近似组成、功能和抗营养特性。这项研究的目的是确定从这些种子中提取的面粉可能具有的任何潜力。水分,5.00和10.36%;纤维素占11.00和15%,碳水化合物占74.41和64.23%。功能性状分析结果表明,2个品种的面粉吸水率分别为2.0 g/g和1.5 g/g;吸油量分别为2.16 g/g和1.83 g/g,起泡量分别为3.79 g/g和3.75 g/g,其中印度品种的各项功能性能均较高,但差异不显著(p >除吸水能力外,其余各值均为0.05)。氰化氢、植酸和胰蛋白酶抑制剂的含量普遍较低,处于安全水平。
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引用次数: 22
Bread Making Potential of Composite Flour of Wheat-Acha (Digitaria exilis staph) Enriched with Cowpea (Vigna unguiculata L. walp) Flour 添加豇豆粉的小麦-柞蚕复合粉的面包生产潜力
Pub Date : 2013-01-01 DOI: 10.1016/S0189-7241(15)30050-3
Olapade A.A. , Oluwole O.B.

Bread-making potentials of composite flours containing 90% wheat and 10% acha enriched with 0-15% cowpea flour were investigated. Proximate composition and functional properties of the blends were studied using AOAC standard methods. Bread loaves were prepared from the blends using the straight dough method and evaluated for loaf height, loaf volume, loaf weight and sensory characteristics. Crude protein, crude fat, crude fibre and ash contents increased significantly (p < 0.05) with increase in level of cowpea flour addition, but moisture content was not significantly (p > 0.05) different among the blends. Functional properties, with exception of bulk density and swelling capacity, were significantly (p < 0.05) different among the blends. Average loaf height and loaf volume decreased significantly (p < 0.05) with increased cowpea flour but loaf weight showed opposite trend with significant (p < 0.05) differences as cowpea flour increased. However, the addition of cowpea flour significantly (p < 0.05) decreased the loaf specific volume but all enriched samples were not significantly (p > 0.05) different. Bread samples from composited blends were rated lower than bread from all wheat bread. Bread loaves from enriched composite flour with up to 10% cowpea flour were acceptable to the panelists.

研究了含有90%小麦和10%阿卡果的复合面粉在0-15%豇豆粉的基础上制作面包的潜力。采用AOAC标准方法研究了共混物的近似组成和功能特性。采用直面团法制备面包,并对其面包高度、面包体积、面包重量和感官特性进行评价。粗蛋白质、粗脂肪、粗纤维和灰分含量显著增加(p <0.05),但水分含量不显著(p >0.05)。除体积密度和膨胀容量外,其功能性能显著(p <0.05)。平均面包高度和面包体积显著下降(p <0.05),但面包重呈显著相反趋势(p <0.05),差异随豇豆粉的增加而增大。然而,豇豆粉的添加显著(p <0.05)降低了面包比容,但所有富集样品的比容均不显著(p >0.05)不同。复合混合物面包样品的评级低于全小麦面包样品。由含有高达10%豇豆粉的强化复合面粉制成的面包被小组成员接受。
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引用次数: 40
Modelling the Effect of Toasting Time on the Functional Properties of Brachystegia eurycoma Flour 烘烤时间对短肌肌肉瘤面粉功能特性影响的模拟研究
Pub Date : 2013-01-01 DOI: 10.1016/S0189-7241(15)30064-3
Ikegwu O.J. , Okechukwu P.E. , Ekumankama E.O. , Okorie P.A. , Odo M.O.

The effect of toasting time on the functional properties of Brachystegia eurycoma flour was investigated. The water absorption capacity, swelling power and solubility index of the flour, increased with increase in toasting time, while the oil absorption capacity and amylose content of the flour decreased with increase in toasting time. The peak viscosity, final viscosity, break down and setback viscosity increased from 134.67 to 176.24 RVU, 460.33 to 650.43 RVU, 57.83 to 120.33 RVU and 405.70 to 581.17 RVU respectively, as the toasting time increased. The pasting temperature decreased from 88.25 to 78.32 °C, as the toasting time increased from 0 to 12 min. Regression models that could be used to adequately express the relationships existing between the functional properties of the flour and toasting time were established.

研究了不同烘焙时间对粗筋面粉功能特性的影响。面粉的吸水能力、溶胀能力和溶解度指数随烘烤时间的延长而增加,而吸油能力和直链淀粉含量随烘烤时间的延长而降低。随着焙烧时间的延长,峰值粘度、最终粘度、破碎粘度和后退粘度分别从134.67 ~ 176.24 RVU、460.33 ~ 650.43 RVU、57.83 ~ 120.33 RVU和405.70 ~ 581.17 RVU增加。糊化温度从88.25℃降低到78.32℃,烘烤时间从0 min增加到12 min。建立了能充分表达面粉功能特性与烘烤时间关系的回归模型。
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引用次数: 4
Mathematical Modelling of Thin Layer Dried Cashew Kernels 薄层干燥腰果果仁的数学建模
Pub Date : 2013-01-01 DOI: 10.1016/S0189-7241(15)30083-7
Asiru W.B. , Raji A.O. , Igbeka J.C. , Elemo G.N.

In this paper mathematical models describing thin layer drying of cashew kernels in a batch dryer were presented. The range of drying air temperature was 70 – 110°C. The initial moisture content of the cashew kernels was 9.29% (d.b.) and the final moisture content was in the range of 3.5 to 4.6% dry-basis. Seven different thin layer mathematical drying models were compared according to their coefficients of determination (R2) mean square error (MSE) and mean relative deviation modulus (P) to estimate drying curves. The effects of the drying air temperature and time on the drying model constants and coefficients were predicted by multiple regression analysis using linear and non-linear type models. The results have shown that among the models, the Page model was found to be the best for describing the drying behaviour of cashew kernels with R2, MSE and P values of 0.9830, 0.00311 and 5.046 respectively.

本文建立了腰果仁在间歇式干燥机中薄层干燥的数学模型。干燥空气温度范围为70 ~ 110℃。腰果仁的初始水分含量为9.29% (d.b.s),干基含量为3.5% ~ 4.6%。比较7种不同薄层干燥数学模型的决定系数(R2)、均方误差(MSE)和平均相对偏差模量(P),估算干燥曲线。采用线性和非线性模型进行多元回归分析,预测了干燥空气温度和干燥时间对干燥模型常数和系数的影响。结果表明,Page模型最能描述腰果仁的干燥特性,其R2、MSE和P值分别为0.9830、0.00311和5.046。
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引用次数: 11
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Nigerian Food Journal
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