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Chemical Treatment of Black Tiger nut Tubers (Cyperusesculentus) Variety to Obtain a Bio-based Product 黑虎坚果块茎(Cyperusesculentus)品种化学处理获得生物基产品
Pub Date : 2021-09-20 DOI: 10.31579/2637-8914/069
Djomdi Djomdi, R. Ejoh, G. Pierre, C. Delattre, P. Michaud, R. Ndjouenkeu
The purpose of this study is to improve microbiological qualities of black tiger nut tubers. Thereby, black variety of tiger nut tubers (Cyperus esculentus) were differently processed in ascorbic acid and alkaline (Ca(OH)2 and Kanwa (alkaline calcium carbonate locally used to soften vegetable)) solutions at different concentrations and temperatures. The effect of these treatments on the water content (swelling), soluble loss, dehulling efficiency and microbial load was investigated. Water content or swelling of the tiger nut soaked in acid and alkaline solutions can be predicted by Peleg model like with brown variety.of C. esculentus tubers. This swelling lead to enhance dehulling efficiency of tuber but caused soluble solute loss overall at the highest temperature of soaking in vitamin C solution. Like soaking in water, the constant of Peleg (k1) decrease with the increase of the temperature of soaking, whereas the constant of capacity of Peleg (k2) is approximately 0.018% for all the conditions. Dehulling efficiency also increased with increasing temperatures (65% at 20°C to 70% at 60°C) for tiger nut tubers soaked in water (0 g/L), dehulling efficiency appeared most strongly affected by concentrations and temperatures of soaking solutions. Like dehulling efficiency, ascorbic solutions has given the best results in term of reduction of microbial load compared to the other soaking solutions. This reduction is considerable at the higher temperatures, reaching a maximum of 4.8 log reduction. Soaking and dehulling treatments gave to black tiger nut tuber effective decontamination in term of microbial load and obtained good microbiological quality for final process.
本研究旨在提高黑虎坚果块茎的微生物品质。因此,在不同浓度和温度的抗坏血酸和碱性(Ca(OH)2和Kanwa(当地用于软化蔬菜的碱性碳酸钙)溶液中,黑色品种虎坚果块茎(Cyperus esculentus)进行了不同的处理。考察了不同处理对玉米的含水量(溶胀)、可溶性损失、脱壳效率和微生物负荷的影响。虎坚果在酸、碱溶液中浸渍后的含水量或溶胀率均可与棕黄品种一样用Peleg模型进行预测。块茎的一种。在维生素C溶液中浸泡的最高温度下,块茎的溶胀导致块茎脱壳效率提高,但整体上造成可溶性溶质损失。与水浸泡一样,Peleg常数(k1)随浸泡温度的升高而减小,而Peleg容量常数(k2)在所有条件下均约为0.018%。0 g/L水浸泡后,脱壳率随温度的升高而升高(20℃时为65%,60℃时为70%),脱壳率受浸泡溶液浓度和温度的影响最大。与脱壳效率一样,与其他浸泡溶液相比,抗坏血酸溶液在减少微生物负荷方面具有最佳效果。在较高的温度下,这种减少是相当可观的,最大减少了4.8 log。浸泡和脱皮处理对黑虎坚果块茎的微生物负荷有较好的去污效果,最终工艺的微生物质量较好。
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引用次数: 0
Review on Macronutrient in Agronomy Crops 农用作物宏量营养素研究进展
Pub Date : 2021-09-20 DOI: 10.31579/2637-8914/062
Isreal Zewide, M. W.
Macronutrients play a very important role in plant growth and development. Three main elements are nitrogen, phosphorus, and potassium (N, P, K) and are required in abundance. They must be readily available through soil medium or fertilizer. The secondary elements are sulfur, calcium, and magnesium (S, Ca, Mg). The problem of low soil fertility and poor plant nutrition does not only affect crop yields but also crop quality. Various nutrient elements influence biochemical processes and eventually affect the overall quality of various crops and their products. Depending on the amount that is available for plant uptake, these nutrients influence crop yields and quality and plants treated with absence of nutrients in the nutrient solution showed that N and Ca were the most limiting nutrients to biomass production The crop quality characteristics mostly reported to be affected by plant nutrition include: proteins, carbohydrate, sucrose and fructose content in grains, root crops, tuber crops and fruits; vitamins like beta-carotene content in fruits and tubers; moisture content at storage in cereal grains, potato tuber density; and frying colours, and fruit weight. It has been noted that essential and beneficial nutrient elements contribute to crop quality through functioning as raw materials for the synthesis of various plant components that have food value to humans and animals.
常量营养素在植物生长发育中起着非常重要的作用。三种主要元素是氮、磷和钾(N、P、K),它们的需求量很大。它们必须易于通过土壤介质或肥料获得。次级元素是硫、钙和镁(S、Ca、Mg)。土壤肥力低,植物营养不良,不仅影响作物产量,而且影响作物品质。各种营养元素影响生物化学过程,最终影响各种作物及其产品的整体质量。根据可供植物吸收的量,这些养分影响作物产量和品质,在营养液中缺乏养分的情况下处理的植物表明,N和Ca是最限制生物量生产的养分。据报道,受植物营养影响的作物品质特征主要包括:谷物、块根作物、块茎作物和水果中的蛋白质、碳水化合物、蔗糖和果糖含量;水果和块茎中-胡萝卜素等维生素的含量;谷物贮藏时的水分含量,马铃薯块茎密度;油炸的颜色,水果的重量。已经注意到,必需和有益的营养元素作为合成对人类和动物具有食物价值的各种植物成分的原料,有助于提高作物质量。
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引用次数: 2
Prevalence of Obesity and Its Association with Socio Demographic Factors in Obese Omani Women in the Age Group of 30-49 Years 30-49岁阿曼肥胖妇女的肥胖患病率及其与社会人口因素的关系
Pub Date : 2021-09-20 DOI: 10.31579/2637-8914/067
M. Seth
Background: Obesity is a severe public health issue that is escalating at an alarming rate around the world. Almost all the developed and developing nations are experiencing this health crisis at varying levels; cultural and socio-demographic factors contributing significantly. Like many other Gulf countries, Oman is also facing increasing trends in obesity and the associated morbidities which have erupted as the most challenging health concerns in Oman, especially in women. Objective: This research was done to see impact of socio demographic factors such as age, occupation, marriage, education, income on obesity in Omani women. Method: A cross sectional study was done on 398 Omani women in the age group of 30 – 49 years with “BMI” (Body Mass Index) above 30. A questionnaire was used to collect socio demographic information of subjects through interview. The anthropometric measurements such as “BMI” and “WHR” (Waist Hip Ratio) were used to classify them as class I, II and III obese statistical models. Results: Sample of 398 women included 38 % in 30-34 years, 22% in 35-40 years, 16% in 40-44 years and 23% in 45-49 years age. 21% having BMI above 40 were classified as class III, 32% were class II (BMI 35-40) and 47% of women were class I obese (BMI 30-35). 60 % of sample was from Muscat and 40% from Batina. 76% of subjects were married, 18 % single and 3 % separated. 57 % of participants were from joint families and 43 % nuclear. 36% graduates, 25% diploma, 25% till high school and 14% middle school. 43% of women were not working and 51% working. 47% of subjects had family income over Rial Omani 2000, 45% between Rial Omani 600-2000 and 8% had income below Rial Omani 600. Conclusion: Results indicate high prevalence of obesity and its association with several socio demographic characteristics. There is an urgent need for launching awareness health program focusing on healthy lifestyle.
背景:肥胖是一个严重的公共卫生问题,在世界范围内正以惊人的速度升级。几乎所有发达国家和发展中国家都在不同程度上经历着这种健康危机;文化和社会人口因素起着重要作用。与许多其他海湾国家一样,阿曼也面临肥胖和相关发病率上升的趋势,这已成为阿曼最具挑战性的健康问题,特别是妇女。目的:本研究旨在了解年龄、职业、婚姻、教育、收入等社会人口因素对阿曼妇女肥胖的影响。方法:对398名年龄在30 - 49岁,身体质量指数(BMI)大于30的阿曼女性进行横断面研究。采用问卷调查的方式,通过访谈收集被试的社会人口学信息。采用“BMI”、“WHR”(腰臀比)等人体测量指标将其分为I、II、III类肥胖统计模型。结果:本组398例女性中,30 ~ 34岁占38%,35 ~ 40岁占22%,40 ~ 44岁占16%,45 ~ 49岁占23%。BMI超过40的女性中有21%属于III类肥胖,32%属于II类肥胖(BMI 35-40), 47%属于I类肥胖(BMI 30-35)。60%的样本来自马斯喀特,40%来自巴蒂纳。76%的研究对象已婚,18%单身,3%分居。57%的参与者来自联合家庭,43%的参与者是大家庭成员。36%毕业生,25%文凭,25%高中毕业,14%初中毕业。43%的女性没有工作,51%的女性有工作。47%的研究对象家庭收入超过2000阿曼里亚尔,45%在600-2000阿曼里亚尔之间,8%的人收入低于600阿曼里亚尔。结论:结果表明肥胖的高患病率及其与一些社会人口统计学特征有关。迫切需要开展以健康生活方式为重点的健康意识项目。
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引用次数: 0
Preparation and Quality Evaluation of Buckwheat Incorporated Bread 荞麦复合面包的制备及品质评价
Pub Date : 2021-08-12 DOI: 10.31579/2637-8914/058
Arjun Ghimire
This study aimed to optimize buckwheat flour in bread. The product formulation was based on the results obtained from the DOE (Design of Expert) v 7.1.5. Buckwheat flour incorporated bread was prepared with the incorporation of buckwheat flour in 10%, 17.5%, 20%, 25%, 30%, 32.5%, and 40% concentration with wheat flour. The physical properties of buckwheat were analyzed along with the different physical properties of the prepared product. The proximate along with iron content and sensory analysis of buckwheat incorporated bread of different concentrations was carried out and the means were compared at a 5% level of significance. The physical properties of buckwheat revealed the l/b ratio of 1.51, bulk density of 70.23 Kg/HL, and 1000 Kernel weight of 22.12g, respectively. Physical analysis of the bread formulations showed that the loaf volume and specific loaf volume decreased while the weight increased with the incorporation of buckwheat flour. The lowest loaf volume of 204 cm3 and specific loaf volume of 1.86cm3/g was recorded for 40% buckwheat incorporated bread with an increased weight of 109.40g. The protein, fat, crude fiber, ash, and iron were found to be 12.55%, 4.89%, 1.56%, 2.43%, and 3.27 mg/100 g respectively, in 25% buckwheat flour incorporated bread. The formulation of 25% buckwheat flour was found to be best in sensory characteristics in terms of color, taste, flavor, crumb appearance, and overall acceptability. Hence, the results concluded that 25% buckwheat incorporated flour bread was the optimum bread formulation for the preparation of composite bread.
本研究旨在优化荞麦粉在面包中的应用。该产品配方基于DOE (Design of Expert) v 7.1.5获得的结果。将荞麦粉分别以10%、17.5%、20%、25%、30%、32.5%和40%的浓度与小麦粉掺入,制备荞麦粉掺入面包。对荞麦的物理性质进行了分析,并对所制产品的不同物理性质进行了分析。对不同浓度荞麦掺入面包进行了近似值、铁含量和感官分析,并在5%的显著水平上进行了均值比较。荞麦理化性状的l/b比为1.51,容重为70.23 Kg/HL,千粒重为22.12g。面包配方的物理分析表明,加入荞麦粉后,面包体积和比面包体积减小,重量增加。添加40%荞麦的面包的最低面包体积为204 cm3,比面包体积为1.86cm3/g,增加重量为109.40g。25%荞麦粉混合面包的蛋白质含量为12.55%,脂肪含量为4.89%,粗纤维含量为1.56%,灰分含量为2.43%,铁含量为3.27 mg/100 g。25%荞麦粉的配方被发现在颜色、味道、风味、面包屑外观和整体可接受性方面的感官特性最好。综上所述,25%荞麦加粉面包是制备复合面包的最佳面包配方。
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引用次数: 1
Benefit and use of Sweet Basil (Ocimum Basilicum l.) In Ethiopia: -A Review 甜罗勒(Ocimum Basilicum l.)的益处和用途在埃塞俄比亚:一篇评论
Pub Date : 2021-08-12 DOI: 10.31579/2637-8914/057
D. Egata
Sweet basil (Ocimum basilicum L.) is an aromatic plant directly used for spice, medicine, feed of honeybee, ornamental and also used as raw material for different industries. Sweet basil is most widely cultivated due to its high economical value, popularity and demands among the economically important species of basil. Sweet basil is widely distributed throughout subtropic and tropical regions and currently widely cultivated in India, Ethiopia, Egypt, Iran, Japan, China and Turkey. Commercially, it extensively cultivated for essential oil production in many continents around the world for its numerous economical, medicinal and aromatic values. In Ethiopia, sweet basil is popular spice plant used by population and each Ethiopian ethnic groups have different names for sweet basil throughout the country. Ethiopian farmers conventionally cultivate and use this crop for house consumption and provide for local market mainly as spice. Sweet basil’s aromatic leaf directly used as flavouring agent in different foods and beverages industries. Oil and oleoresin of sweet basil indirectly widely used for flavour and fragrance in the food, pharmaceutical, cosmetic, and aromatherapy industries. It also used extensively in pharmaceutical, cosmetics, aromatherapy and food industry. The other use of sweet basil is for production of honey and can be used as ornamental plant. Sweet basil is very important crop that can play great role on the economy of Ethiopian farmers. Generally, Sweet basil plant is can be considered as; easily available, easy to grow, have multipurpose advantages that should be produced as high value crop.
罗勒(Ocimum basilicum L.)是一种芳香植物,可直接用于香料、医药、蜜蜂饲料、观赏和不同工业的原料。甜罗勒因其较高的经济价值,在经济上重要的罗勒品种中受欢迎和需求而被广泛种植。罗勒广泛分布于亚热带和热带地区,目前在印度、埃塞俄比亚、埃及、伊朗、日本、中国和土耳其广泛种植。在商业上,由于其众多的经济、药用和芳香价值,它在世界许多大洲被广泛种植用于精油生产。在埃塞俄比亚,甜罗勒是人们常用的香料植物,每个埃塞俄比亚民族在全国各地都有不同的名字。埃塞俄比亚农民传统上种植和使用这种作物供家庭消费,并主要作为香料提供给当地市场。甜罗勒的芳香叶直接用作各种食品和饮料工业的调味剂。甜罗勒的油和油树脂间接广泛用于食品、制药、化妆品和芳香治疗行业的香精和香料。广泛应用于医药、化妆品、香薰、食品等行业。甜罗勒的另一个用途是生产蜂蜜,可以用作观赏植物。甜罗勒是一种非常重要的作物,对埃塞俄比亚农民的经济起着重要作用。一般来说,罗勒植物是可以被认为是;易得,易种植,具有多用途的优点,应作为高价值作物生产。
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引用次数: 7
Morphological Alterations and Virulence Gene Expression of Listeria Monocytogenes Cells in Response to Gamma Irradiation Treatments γ辐照对单核增生李斯特菌细胞形态变化及毒力基因表达的影响
Pub Date : 2021-08-12 DOI: 10.31579/2637-8914/055
Hamouda Elabed
Gamma irradiation is one of the most popular ray treatments in food industry. It's used to control micro-organisms proliferation in a wide range of products however the response of bacteria to low doses is still unknown. In this study we mainly focus on morphological alteration and virulence gene expression in Listeria monocytogenes after gamma irradiation treatments. The atomic force micrographs (AFM) showed that 0.5 kGy dose has no effect on the membrane morphology of L. monocytogenes. However, after 0.7 kGy treatment, the cells lost their typical shape and smooth membrane and 1 kGy dose was totally destructive. Moreover, membrane fatty acid composition was analyzed by the chromatographic method after different gamma doses. Significant modifications on fatty acids composition were detected in the irradiated strain showing a novo synthesis of membrane lipids: C12:0; C14:0; C15:0; C16:0 and C18:0. In addition, we reported an increase of the saturated fatty acid, essential for membrane adaptation under stress conditions. The expression levels of three virulence genes (hlyA, fri and prfA) were studied in the same conditions using real-time PCR technique. The analysis revealed that both prfA and fri genes were up-regulated after gamma treatment. The induction of prfA, which is a regulator gene, may affect the expression other genes controlling the adaptive form in the treated strain. This study open prospects for further researches to explain the regulatory mechanisms of the adaptive response in Listeria monocytogenes when exposed to sublethal irradiation-stress.
射线辐照是食品工业中最常用的射线处理方法之一。它被用于控制各种产品中微生物的繁殖,但细菌对低剂量的反应尚不清楚。本研究主要研究了γ辐照后单核增生李斯特菌的形态变化和毒力基因表达。原子力显微镜(AFM)显示,0.5 kGy剂量对单核增生乳杆菌的膜形态没有影响。0.7 kGy处理后,细胞失去了原有的形态和光滑的细胞膜,1 kGy剂量完全破坏。此外,用色谱法分析了不同γ剂量后的膜脂肪酸组成。辐照菌株的脂肪酸组成发生了显著变化,显示膜脂的新合成:C12:0;C14:0;C15:0;C16:0和C18:0。此外,我们报道了饱和脂肪酸的增加,这是在胁迫条件下膜适应所必需的。采用实时荧光定量PCR技术,在相同条件下检测3个毒力基因(hlyA、fri和prfA)的表达水平。分析显示,伽玛治疗后prfA和fri基因均上调。prfA是一种调控基因,其诱导可能会影响其他控制菌株适应形式的基因的表达。本研究为进一步研究单核增生李斯特菌在亚致死辐照胁迫下适应性反应的调控机制开辟了前景。
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引用次数: 0
Analysis of the relationship between nutrition and cancer: Gastronomic options for prevention 营养与癌症的关系分析:预防的饮食选择
Pub Date : 2021-08-12 DOI: 10.31579/2637-8914/054
M. Pal
The relationships between tumorigenesis and nutrition are difficult to analyse. In addition to the composition of foods, a number of environmental and other factors modify the results of dietary observations. The nutritional factors can also be direct acting carcinogens, promoters and anti-promoters in the process of tumor formation. The most important promoters are excessive energy, high fat and animal protein intake, low fibre intake, and excessive alcohol consumption. The dietary fibre, antioxidant vitamins, antioxidant minerals and other elements play a prominent role among anti-promoters in foods. The primary purpose of the manuscript is to analyse these promoters, anti-promoters, and direct acting carcinogens. Furthermore, we prepare and present foods that significantly contribute to a tumor preventive diet and support the active defence of the human body with the help of gastronomic science.
肿瘤发生与营养之间的关系很难分析。除了食物的成分外,许多环境和其他因素也会改变饮食观察的结果。在肿瘤形成过程中,营养因子还可直接作用致癌物、促进剂和抗促进剂。最重要的促发因素是过多的能量、高脂肪和动物蛋白摄入、低纤维摄入和过量饮酒。膳食纤维、抗氧化维生素、抗氧化矿物质等元素在食品抗促进剂中起着突出的作用。本文的主要目的是分析这些启动子、抗启动子和直接作用致癌物。此外,我们在烹饪科学的帮助下,准备和呈现对肿瘤预防饮食有重要贡献的食物,并支持人体的积极防御。
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引用次数: 0
Analysis of Functional Foods and Active Ingredients used in Sports 运动中使用的功能食品和活性成分分析
Pub Date : 2021-08-12 DOI: 10.31579/2637-8914/059
M. Pal
There are more and more group sports events and individual sporting opportunities in the world. In this case, a number of factors need coordinated organ function. This requires an increased attention to the protection of the athlete's health.The regular consumption of functional foods with physiologically active substances found in them can stimulate organ function. Physiologically active ingredients in these functional foods include minerals, macronutrients, conjugated linoleic acid, coenzyme Q10, probiotics, prebiotics, omega-3 fatty acids, choline, and vitamins. Their effect is also beneficial in the functioning of the movement system, circulatory system, visceral organs, regulatory system and sensory system. Our publication is aimed to analyze the organ systems used in the physical activity, the functional foods that affect them, and the active ingredients in them. It is imperative to use nutritionally rich foods for keeping good health.
世界上有越来越多的团体运动项目和个人运动机会。在这种情况下,许多因素需要协调的器官功能。这就需要更加重视对运动员健康的保护。经常食用含有生理活性物质的功能食品可以刺激器官功能。这些功能性食品中的生理活性成分包括矿物质、常量营养素、共轭亚油酸、辅酶Q10、益生菌、益生元、omega-3脂肪酸、胆碱和维生素。它们的作用也有利于运动系统、循环系统、内脏器官、调节系统和感觉系统的功能。我们的出版物旨在分析身体活动中使用的器官系统,影响它们的功能食品,以及其中的有效成分。为了保持身体健康,食用营养丰富的食物是必不可少的。
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引用次数: 0
Wonders of International Gastronomy: French, Italian, Hungarian, Indian and Chinese Cuisine 国际美食奇观:法国、意大利、匈牙利、印度和中国美食
Pub Date : 2021-06-25 DOI: 10.31579/2637-8914/051
M. Pal
With the growing importance of gastronomic science, gastronomic workshops are also playing an important role worldwide. These workshops do not only specialize in the preparation and presentation of healthy eating, sports nutrition, or special diets. Gastronomy experts and nutritionists are also turning to the cuisines of nations with increasing curiosity. In order to provide a comprehensive overview of this, we present the characteristics of French, Italian, Hungarian, Indian, and Chinese cuisine in our manuscript. In addition, we prepare and present dishes that also serve as the basis of gastronomic workshops in accordance with international cuisines. These dishes include: Croassant with almond plant-based drink; Home-made pizza with basil, tomatoes and mozzarella; Hungarian Bean Soup; Home-made kebab with spicy chicken; Chinese noodles. We hope to contribute to the knowledge of international gastronomy and to the global health development with the prepared food.
随着烹饪科学的日益重要,烹饪研讨会也在世界范围内发挥着重要作用。这些讲习班不仅专门准备和介绍健康饮食、运动营养或特殊饮食。美食专家和营养学家也怀着越来越大的好奇心,开始研究各国的美食。为了提供一个全面的概述,我们在我们的手稿中提出了法国、意大利、匈牙利、印度和中国美食的特点。此外,我们准备和呈现的菜肴也作为根据国际美食烹饪工作坊的基础。这些菜肴包括:杏仁植物饮料牛角面包;用罗勒、西红柿和马苏里拉奶酪自制披萨;匈牙利豆汤;自制辣鸡串;中国面条。我们希望通过预制食品为国际美食知识和全球健康发展做出贡献。
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引用次数: 0
Bio-Optimized Curcuma as Adjuvant Therapy of Osteoarthritis and Its Influence on Gene Regulation (Sirt1), Metabolic Inflammation and Associated Symptoms 生物优化姜黄作为骨关节炎辅助治疗及其对基因调控(Sirt1)、代谢性炎症和相关症状的影响
Pub Date : 2021-06-25 DOI: 10.31579/2637-8914/052
S. Bianchi
Background and aim: Osteoarthritis (OA) is a chronic degenerative disease characterized by an inflammatory state and significant oxidative stress. As curcuma is known for its anti-inflammatory and antioxidant activities, it could be used as alternative therapy next to or together with the standard treatment. This treatment consists of analgesics, steroids or non-steroidal anti-inflammatory drugs (NSAIDs) to reduce pain and inflammation related symptoms. The current study investigates the effect of bio-optimized curcuma on genetic (SIRT1) and metabolic regulation of inflammation and associated symptomatology in patients with osteoarthritis. Materials and methods: In the in vitro study, Hela human cells were seeded in 12-well plates, incubated with curcuma at different concentrations and incubated for 3, 6 and 24 hours. The targeted protein expression/phosphorylation was evaluated by immunoblotting, while cytotoxicity tests were performed by CellTiter-Blue Cell Viability Assay. In the in vivo study, a total of 33 patients were recruited and divided into 3 subgroups based on the treatment: standard treatment (ST), ST + curcuma and ST + curcuma + Vitamin D (2000UI). The health status (SF36) and osteoarthritis index (WOMAC score) were analyzed at 0; 1,5 and 3 months with blood sampling at 0 and 3 months for the evaluation of inflammation markers, 25 (OH) VitD and SIRT1. Results: in vitro data showed no statistically significant decrease (p>0.05) in the number of viable cells. The expression of SIRT1 and the activation of AMP activated protein kinase (AMPK) were significantly increased in all experimental groups compared with the control group (p<0.001). A significant increase in 25(OH) VitD values in the ST + curcuma + VitD group (p <0.007) and in SIRT1 in all groups taking curcuma (p <0.001) was shown. Also for IL-1 (p=0.031), IL-6 (p=0.031) and IFN-γ (p=0.013), all groups taking curcuma showed significantly lower inflammatory markers with no added value of vitamin D. Conclusions: Curcuma as an adjuvant to ST leads to a positive modulation of the SIRT1 pathway, a significant decline of blood inflammatory markers and a better osteoarthritis outcome. Osteoarthritis, a chronic degenerative disease characterized by an inflammatory state and significant oxidative stress Curcuma as alternative therapy next to or together with the standard treatment Effect of bio-optimized curcuma on genetic and metabolic regulation of inflammation Positive modulation of SIRT1 pathway, decline of blood inflammatory markers and a better OA outcome
背景与目的:骨关节炎(OA)是一种以炎症状态和显著氧化应激为特征的慢性退行性疾病。姜黄因其抗炎和抗氧化活性而闻名,它可以作为标准治疗的替代疗法或与标准治疗一起使用。这种治疗包括止痛剂、类固醇或非甾体抗炎药(NSAIDs),以减轻疼痛和炎症相关症状。本研究旨在探讨生物优化姜黄对骨关节炎患者炎症和相关症状的遗传(SIRT1)和代谢调节的影响。材料与方法:体外实验中,将Hela人细胞接种于12孔板中,与不同浓度的姜黄共孵育3、6、24小时。免疫印迹法评估靶向蛋白表达/磷酸化水平,细胞毒性试验采用CellTiter-Blue细胞活力测定法。在体内研究中,共招募了33例患者,并根据治疗方法分为标准治疗(ST)、ST +姜黄和ST +姜黄+维生素D (2000UI) 3个亚组。健康状况(SF36)和骨关节炎指数(WOMAC评分)在0时进行分析;1、5和3个月时,分别在0和3个月采血,评估炎症标志物、25 (OH) VitD和SIRT1。结果:体外数据显示,活细胞数无统计学意义(p < 0.05)。与对照组相比,各实验组SIRT1的表达和AMP活化蛋白激酶(AMPK)的活化均显著升高(p<0.001)。结果显示,ST +姜黄+ VitD组25(OH) VitD值显著升高(p <0.007),所有姜黄组的SIRT1显著升高(p <0.001)。此外,对于IL-1 (p=0.031)、IL-6 (p=0.031)和IFN-γ (p=0.013),服用姜黄的各组炎症标志物均显著降低,而维生素d没有增加。结论:姜黄作为ST的佐剂,可调节SIRT1通路,显著降低血液炎症标志物,改善骨关节炎的预后。骨关节炎,一种以炎症状态和显著氧化应激为特征的慢性退行性疾病姜黄作为标准治疗的替代或联合治疗生物优化姜黄对炎症的遗传和代谢调节作用SIRT1途径的阳性调节,血液炎症标志物的下降,OA预后更好
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Nutrition and Food Processing
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