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Isolation and characterization of probiotic bacteria from fruit pulp, screening of probiotic properties and production of bacteriocin and probiotic curd 果蔬果肉中益生菌的分离与鉴定、益生菌特性的筛选及细菌素和益生菌凝乳的生产
Pub Date : 2023-07-19 DOI: 10.17352/ojbs.000036
Kamaliya Dhanvanti B, Javia Bhumi M, Gadhvi Megha S, Dudhagara Dushyant R
The present study was investigated to isolate probiotic lactic acid bacteria from Actinidia deliciosa (kiwi fruit) pulp. A total of eight isolates were found and two of them were probiotic LAB strains. The both A2 and A5 isolates strains were Gram-positive, catalase and oxidase negative, non-spore forming, non-motile, rod or short cocci shaped bacteria. The A2 and A5 strains both survived well in conditions of low pH, 0.4% phenol and high bile salt concentrations. They could grow at different temperatures and were resistant to different NaCl concentrations. A study was also conducted on the various probiotic and safety attributes of isolates. Bacteriocins, a kind of ribosomal protein produced by LAB, were estimated using the Folin-Lowery method. The formation of bacteriocins was influenced by various physical and chemical factors. The result demonstrates that the optimum conditions for the production of bacteriocins by the A2 strain were glucose as carbon source, pH 6.5, 2% NaCl and 1% bile salts. For the A5 strain, sucrose as a carbon source, pH 6.5, 1% NaCl and 1% bile salt were optimum conditions for bacteriocin production. Study was also conducted on exopolysaccharide (EPS) production, adhesion characteristics, antagonistic activity and antibiotic sensitivity. Probiotic curd (Dahi) formation was done and several parameters of curd like pH, total lactic acid concentration and water-holding capacity were all investigated.
本研究从猕猴桃果肉中分离出益生菌乳酸菌。共分离到8株,其中2株为益生菌LAB菌株。A2和A5菌株均为革兰氏阳性,过氧化氢酶和氧化酶阴性,不形成孢子,不运动,棒状或短球菌状细菌。A2和A5菌株在低pH、0.4%苯酚和高胆盐浓度条件下均存活良好。它们能在不同温度下生长,对不同NaCl浓度具有抗性。并对菌株的各种益生菌和安全特性进行了研究。细菌素(bactericins)是一种由LAB产生的核糖体蛋白,用Folin-Lowery法测定。细菌素的形成受多种理化因素的影响。结果表明,A2菌株产菌素的最佳条件为葡萄糖为碳源、pH 6.5、2% NaCl和1%胆盐。对于A5菌株,蔗糖为碳源,pH为6.5,1% NaCl和1%胆汁盐是产菌素的最佳条件。并对其胞外多糖(EPS)的产生、粘附特性、拮抗活性和抗生素敏感性进行了研究。研究了益生菌凝乳的形成,并对凝乳的pH、总乳酸浓度、保水能力等参数进行了研究。
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引用次数: 0
Evaluation of the toxicity of aqueous extracts of Aframomum melegueta, Picralima nitida, and Garcinia cola in Wistar rats 木香、牛蒡、藤黄水提物对Wistar大鼠的毒性评价
Pub Date : 2023-05-11 DOI: 10.17352/ojbs.000035
Tjb Kabongo, LA Luvingisa, MP Ngoie, M. Musuyu, M. Musunga, MW Kabamba, PP Pyana
In order to determine the risks to human health associated with the use of certain medicinal plants, including Aframomum melegueta, Garcinia kola and Picralima nitida in a preclinical evaluation of the resistance, a pool of these three aqueous extracts was given once daily for ten days by gavage in Wistar rats. Haematological and biochemical analyzes after oral administration revealed a decrease in certain hepatic biomarkers such as glucose, Alanine Aminotransferase (ALT), etc., and renal biomarkers such as urea, creatinine, and creatinine kinase); increase in certain biomarkers such as Aspartate Transaminase (AST), an indicator of kidney and liver capacity.
为了确定与使用某些药用植物有关的人类健康风险,包括在耐药性临床前评估中使用阿夫拉蒙(Aframomum melegueta)、藤黄(Garcinia kola)和小木犀草(Picralima nitida),在Wistar大鼠中每天一次灌胃这三种水提取物,持续十天。口服给药后的血液学和生化分析显示,某些肝脏生物标志物(如葡萄糖、丙氨酸转氨酶(ALT)等)和肾脏生物标志物(如尿素、肌酐和肌酐激酶)降低;某些生物标志物如谷草转氨酶(AST)增加,AST是肾脏和肝脏容量的指标。
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引用次数: 0
Some aspects of physiological studies of two reef building corals in the red sea 红海两种造礁珊瑚的生理研究
Pub Date : 2023-04-13 DOI: 10.17352/ojbs.000034
Floos Yahya AM, AAl-Sofyani Abdulmohsin
Some aspects of the physiology of the corals Seriatopora hystrix and Lobophyllia corymbosa described in this paper. At the study site, the lowest mean of seawater temperature is 27.42 °C and 27.17 °C at 5m and 10m depths respectively during winter, while the maximum mean of seawater temperature was 32.67 °C and 31.17 °C in 5 m and 10 m depths respectively during summer. L. corymbosa, had a lower number of zooxanthellae 0.05×105 , 0.43×105 , 0.06×105 and 0.46×105 mg-1 dry tissue weight than S. hystrix 0.19 ×105 , 5.1 ×105 , 0.27×105 and 9.59×105 mg-1 dry tissue weight in two season and two depths respectively. The mean respiration rate of whole colonies of S. hystrix was higher than that of L. corymbosa at the same depths both in summer and winter. The mean dark respiration rate decreases with increasing depths. The mean photosynthesis vs irradiance curves of S. hystrix and L. corymbosa were plotted to the hyperbolic tangent function (Chalker,1981) for summer and winter season. The growth rate was linear during each period of measurement during summer, the highest mean daily skeletal growth rate of S. hystrix was 2.3 ± 1.3 (20) mg.skel.d-1 in 10m depth and it was 1.6 ± 0.5 (21) mg .skel.d-1 at 5m depth. Whilst during winter, the lowest was 1.9 ± 0.96 (20) mg .skel. d-1 at 10m and also lowest (1.5 ± 0.7 (20) mg .skel. d-1) at 5m depth. The growth rate of the two species was lower in the winter than in summer.
本文介绍了海丝珊瑚(Seriatopora hystrix)和雪叶珊瑚(Lobophyllia corymbosa)生理的一些方面。研究点冬季5m和10m深度海水温度最低平均值分别为27.42°C和27.17°C,夏季5m和10m深度海水温度最高平均值分别为32.67°C和31.17°C。在2个季节和2个深度上,芦苇的虫黄藻数量0.05×105、0.43×105、0.06×105和0.46×105 mg-1干组织重分别低于黄棘的0.19 ×105、5.1 ×105、0.27×105和9.59×105 mg-1干组织重。在夏季和冬季,在相同深度下,全菌落的平均呼吸速率均高于冠状螺旋藻。平均暗呼吸速率随深度的增加而降低。利用双曲正切函数(Chalker,1981)绘制了夏、冬两季的平均hystrix和L. corymbosa光合作用与辐照度曲线。夏季各测量期的骨骼生长速率呈线性关系,最高平均日骨骼生长速率为2.3±1.3 (20)mg. skl。10m深度D-1, 1.6±0.5 (21)mg . skl。5m深度D-1。冬季最低,为1.9±0.96 (20)mg . skl。D-1在10m处也最低(1.5±0.7 (20)mg . skl)。D-1)水深5m。两种植物在冬季的生长速率均低于夏季。
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引用次数: 0
Updates in management of abdominal sepsis 腹部败血症治疗的最新进展
Pub Date : 2023-03-29 DOI: 10.17352/ojbs.000033
de Araujo Borges Mirla Fontes, de Sousa Lacerda Roosveni, de Sousa Oliveira Igor, Ferreira Sávio Benvindo
Sepsis is an organ dysfunction caused by a disproportionate host response to infection. The condition is responsible for 1 in every 3 admissions to an intensive care unit (ICU) and about 6 million deaths worldwide. As a result of the imbalance in the inflammatory response, immune dysfunction, mitochondrial damage and other pathophysiological processes, sepsis progresses extremely. This research aimed to search the literature for updates inherent to the therapeutic approach of patients with diseases secondary to intra-abdominal sepsis. The present work is a narrative review with a bibliographical survey carried out in the PUBMED and Virtual Health Library (VHL) research bases, through the search strategy using the following descriptors, in both virtual libraries: “sepsis OR surviving sepsis” AND “intra abdominal infection” AND “therapeutic”. At the end of reading the articles in full, ten papers were selected, from which it was observed that new recommendations regarding volume operative source control, and use of additional therapies therapy. The open abdomen technique is being further explored, as well as more in-depth studies on new drugs for this condition, such as acetylcholinesterase inhibitors, and the use of glucocorticoids became contraindicated, due to low scientific evidence. Therefore, it is concluded that, as it is a medical emergency with high mortality, it is relevant to develop scientific research aimed at the effectiveness, feasibility and safety of new forms of management.
脓毒症是一种器官功能障碍,由宿主对感染的不成比例的反应引起。重症监护病房(ICU)每3名入院患者中就有1人死于该病,全世界约有600万人死于该病。由于炎症反应失衡、免疫功能紊乱、线粒体损伤等病理生理过程,脓毒症进展极为严重。本研究旨在检索文献,了解腹内败血症继发疾病患者治疗方法的更新。目前的工作是在PUBMED和虚拟健康图书馆(VHL)研究基地进行的文献调查的叙述性回顾,通过使用以下描述符的搜索策略,在两个虚拟图书馆:“败血症或幸存的败血症”和“腹腔内感染”和“治疗”。在全文阅读结束时,选择了10篇论文,从中观察到关于体积、手术源控制和使用额外疗法的新建议。开腹技术正在进一步探索中,针对这种情况的新药如乙酰胆碱酯酶抑制剂的研究也越来越深入,由于科学证据不足,糖皮质激素的使用成为禁忌症。因此,作为一种死亡率高的医疗急症,有必要开展针对新管理形式的有效性、可行性和安全性的科学研究。
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引用次数: 0
A Literature Review of International Bioeconomy Strategies 国际生物经济战略研究综述
Pub Date : 2022-10-20 DOI: 10.17352/ojbs.000032
Jiang Tian-Hang, Yang Meng
Since the 21st century, developed countries have paid increasing attention to bioeconomy strategies. In particular, since the spread of the New Crown Pneumonia epidemic, global biotechnology has surged, and the impact of significant public health and safety events has continued to escalate. Bioeconomy strategies have received unprecedented attention. In this context, sorting out and studying international bioeconomy strategies is essential. Therefore, this paper reviews selected national bioeconomy strategies and explored their characteristics.
21世纪以来,发达国家越来越重视生物经济战略。特别是,自新冠肺炎疫情蔓延以来,全球生物技术激增,重大公共卫生和安全事件的影响继续升级。生物经济战略受到了前所未有的关注。在此背景下,整理和研究国际生物经济战略是必不可少的。因此,本文回顾了国家生物经济战略的选择,并探讨了其特点。
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引用次数: 0
Population analysis and spring count of the number of geese in the Amur region (Russian Federation) 阿穆尔河地区(俄罗斯联邦)鹅种群分析及春季计数
Pub Date : 2022-10-08 DOI: 10.17352/ojbs.000031
Senchik Aleksandr, Kolischuk Sergey, Pavlov Andrey
Hunting for waterfowl is the most massive for amateur hunters in Russia. Hunting starts in spring and autumn, and the largest number of hunters take part in it, due to its popularity, low economic cost for hunters, as well as the possibility of using a hunting dog. In this regard, the role of counting waterfowl in autumn is important. The organization and conduct of accounting work should be carried out by hunting organizations annually, within certain calendar terms, and at a high-quality level.
在俄罗斯,捕猎水禽是业余猎人最大的任务。狩猎开始于春季和秋季,由于其受欢迎程度,猎人的经济成本低,以及使用猎犬的可能性,参加狩猎的猎人人数最多。在这方面,秋天的水禽计数的作用是重要的。会计工作的组织和实施应由狩猎组织每年在一定的日历期限内以高质量的水平进行。
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引用次数: 0
Histological characterization of sexual reproduction in the coral Pocillopora damicornis (Coelenterata: Scleractinia) from the Red Sea 红海珊瑚poillopora damicornis有性生殖的组织学特征(腔肠目:sclactinia)
Pub Date : 2022-08-26 DOI: 10.17352/ojbs.000030
Floos Yahya AM
The scleractinian coral is common along the Red Sea coast, and its reproductive mode and period of reproduction were assessed using histological preparations. the sexuality, and reproductive mod timing of reproductive of pocillopora damicornis from adjacent to the fringing reefs of the Ubhur Creek in the Red Sea, were assessed using a serial histological section. Sexual reproduction in pocillopora damicornis a shallow water hermatypic coral was studied from December 2011 to November 2012. pocillopora damicornis is a simultaneous hermaphrodite with ovary and testis in the project into the body cavity on the same mesentery. Sperm and eggs were usually released simultaneously from the same polyp. The onset of the reproductive period of pocillopora damicornis was found to be limited (April to May). In the number of eggs and testes observed in this period, the gonads were found in the polyps. The pocillopora damicornis egg size ranged from 49.80 µm (in March) to 125.0 µm (in May). Four stages were chosen, to reflect very immature ovaries, the early stages of oocyte development, ova near maturity, and mature ova, and also four distinct stages of sperm development were identified. The state of gonads development (eg. testis and eggs) was measured by a calibrated eyepiece micrometer of a compound light microscope. Zooxanthellae were presented in the mature oocytes in pocillopora damicornis. This study aimed to examine the reproduction mode and timing of pocillopora damicornis.
研究了红海沿岸常见的硬核珊瑚的繁殖方式和繁殖周期。利用连续组织学切片对红海乌布尔河(Ubhur Creek)沿岸礁石附近的达米角波鲷(pocillopora damicornis)的性别和繁殖模式进行了评估。2011年12月至2012年11月对一种浅水两性珊瑚进行了有性生殖研究。大角Pocillopora damicornis是同时雌雄同体,卵巢和睾丸在同一肠系膜上投射到体腔内。精子和卵子通常同时从同一个息肉中释放出来。大角圆孔虫的繁殖期开始时间有限(4 ~ 5月)。在这一时期观察到的卵和睾丸的数量中,性腺在息肉中发现。大角圆孔蝇卵大小为49.80µm(3月)~ 125.0µm(5月)。选择了四个阶段,分别反映了非常不成熟的卵巢、卵母细胞发育的早期阶段、接近成熟的卵子和成熟的卵子,并确定了精子发育的四个不同阶段。性腺发育状态性腺发育的状态。睾丸和卵子)测量由校准目镜千分尺的复合光学显微镜。在大角圆孔虫成熟卵母细胞中存在虫黄藻。本研究的目的是研究大角圆孔虫的繁殖方式和繁殖时间。
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引用次数: 0
An assessment of indigenous knowledge of apitherapy in the Oromia region, Ethiopia 对埃塞俄比亚奥罗米亚地区蜜蜂疗法土著知识的评估
Pub Date : 2022-06-29 DOI: 10.17352/ojbs.000029
G. Meseret, Kebebe Deressa, Demto Teferi
Apitherapy is the use of bee products such as honey, pollen, propolis, royal jelly, bee venom, and wax is used to prevent or treat illness and promote healing. The objectives of this study are to assess and document indigenous knowledge on the medicinal values of bee products in Ethiopia and generate baseline information on indigenous practices of the medicinal value of beehive products for further study. This study was conducted in beekeeping potential areas of Oromia Regional state by selecting seven zones and from each zone two districts and a total of 14 districts (Woreda) based on their potential for beekeeping, the existence of long-aged bee-keepers and accessibility. using a purposive sampling method and questionnaires were distributed to the elders and well-known traditional healers of the study areas. In answering the questionnaires 22-100 ages dwellers or respondents participated. The survey was conducted in 7 zones namely Jimma, Ilu ababora, West wollega, East wollega, East Shoa, West shoa, and Buno Bedele. Simple descriptive statistics were used to analyze the data obtained by collected questionnaires. The result of the study showed that 49.3% of Apis mellifera honey and 31.9% of stingless bee honey, majorly used bee products for apitherapy, and 70.3 % of the bee products used using traditional collection method. According to this study’s results, the traditional drug was prepared from honey in liquid form which comprises the biggest portion mark of 63.4% of respondents. The majority of the respondents strongly agree with bee products as a treatment for different diseases such as asthma, cough, common cold, TB, and wounds. A major challenge faced during using of bee products as medicine is the absence of awareness of apitherapy as it was reported by 28.8%. of the respondents. Thus, it can be concluded that bee products are well-known apitherapy in the Oromia region and it was recommended that awareness creation of bee products as apitherapy and further research on the medicinal value of beehive products for confirmation as medicine for different diseases since it may be a solution for various diseases resisting modern drugs.
蜜蜂疗法是使用蜂产品,如蜂蜜、花粉、蜂胶、蜂王浆、蜂毒和蜂蜡,用于预防或治疗疾病并促进愈合。本研究的目的是评估和记录埃塞俄比亚关于蜂产品药用价值的土著知识,并生成关于蜂窝产品药用价值的土著实践的基线信息,以供进一步研究。本研究在奥罗米亚州的养蜂潜力区进行,根据其养蜂潜力、高龄养蜂人的存在和可达性,从每个区中选择两个区,共14个区(wooreda)。采用有目的的抽样方法,对研究地区的老年人和知名传统治疗师进行问卷调查。在回答问卷22-100年龄的居民或受访者参与。该调查在7个地区进行,即Jimma、Ilu ababora、West wollega、East wollega、East Shoa、West Shoa和Buno Bedele。采用简单描述性统计方法对收集的问卷数据进行分析。研究结果表明,49.3%的蜜蜂蜂蜜和31.9%的无刺蜜蜂蜂蜜主要使用蜂产品进行蜂疗,70.3%的蜂产品使用传统采集方法。根据这项研究的结果,传统药物是由蜂蜜制成的液体形式,占63.4%的受访者的最大比例。大多数受访者强烈同意蜂产品可以治疗不同的疾病,如哮喘、咳嗽、普通感冒、结核病和伤口。在使用蜂产品作为药物期间面临的一个主要挑战是缺乏对蜂疗的认识,据报道有28.8%。在受访者中。综上所述,蜂产品在奥罗米亚地区是一种知名的养蜂疗法,建议提高人们对蜂产品作为养蜂疗法的认识,并进一步研究蜂产品的药用价值,以确认其作为不同疾病的药物,因为它可能是多种疾病抵抗现代药物的解决方案。
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引用次数: 0
Screening and selection of Brachiaria brizantha accessions for forage values under irrigation at Wondo Genet, Sidama, Ethiopia 埃塞俄比亚西达马Wondo Genet灌溉条件下粗棘腕毛虫牧草的筛选和选择
Pub Date : 2022-06-27 DOI: 10.17352/ojbs.000028
Getiso Aman, Mijena Diribi, Shanku Edao
The experiment was conducted to evaluate different Brachiaria brizantha accessions at the Wondo genet agricultural research center to identify species/accessions so as to use the better performing ecotype for wider distribution among livestock producer communities in the country. Seven Brachiaria grass accessions (B. brizantha, 13151; B. brizantha, 13368; B. brizantha, 13653; B. brizantha, 16550; B. humidicola, 9222; B. brizantha, 13379 and B. mutica,) were used as treatments in randomized complete block design with three replications. The overall dry matter yield for B. humidicola, 9222 (5.95t/ha), and B. mutica, (5.61t/ha) accessions didn’t show a significant difference (p > 0.05) but significantly (p < 0.05) lower than all other accessions. Mean plant heights for the accessions were significantly different (p < 0.05) throughout the harvesting cycle period and overall yield with a lower plant height of B. mutica (63.2 cm) while non-significant (p > 0.05) overall mean height was recorded among other accessions. B. brizantha, 13151; B. brizantha, 13368; B. brizantha, 13653; B. brizantha, 16550 and B. brizantha, 13379 accessions outperformed in terms of overall dry matter yield. Further studies on agronomic performances of promising B. brizantha accessions under on-farm conditions and nutritional evaluation involving live-animal experiments are recommended.
在Wondo基因农业研究中心对不同的bribriaria brizantha品种进行了评价,以确定品种/品种,以便在全国畜牧生产社区中使用性能较好的生态型。Brachiaria grass资料7份(B. brizantha, 13151;B. brizantha, 13368;B. brizantha, 13653;B. brizantha, 16550;B.湿润菌,9222;采用3个重复的随机完全区组设计,以棘球菌、13379和多棘球菌为处理。总干物质产量9222 (5.95t/ha)和5.61t/ha的差异不显著(p > 0.05),但显著(p < 0.05)低于其他所有材料。各品系的平均株高在采收期和总产量上差异显著(p < 0.05),但不同品系间的平均株高差异不显著(p > 0.05),不同品系间的平均株高差异显著(p < 0.05)。B. brizantha, 13151;B. brizantha, 13368;B. brizantha, 13653;brizantha, 16550和brizantha, 13379在总体干物质产量方面表现较好。建议在农场条件下进一步研究有前途的刺花材料的农艺性能和包括活体动物实验的营养评价。
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引用次数: 1
Salute to Semyon M. Hertz (1947-2020), an outstanding engineer-radio physicist, his social life and contribution to photosynthetic science 向谢yon M. Hertz(1947-2020)致敬,杰出的工程师和无线电物理学家,他的社会生活和对光合作用科学的贡献
Pub Date : 2021-12-20 DOI: 10.17352/ojbs.000027
Biel Karl Y, Fomina Irina R
The article is dedicated to the memory of Semyon M. Hertz, a talented radiophysicist engineer and remarkable person who left us prematurely at the age of 74. He devoted most of his scientific activities to biology, primarily phototrophic organisms, using radionuclide substances. The main achievements in the study of photosynthesis made thanks to his physical and mathematical calculations and engineering developments, are described. The ability to overcome difficulties, fortitude, vitality, and optimism of Semyon Hertz were noted.
这篇文章是为了纪念谢yon M. Hertz,一位才华横溢的放射物理学家工程师和杰出的人,他在74岁时过早地离开了我们。他的大部分科学活动都致力于生物学,主要是利用放射性核素物质的光养生物。由于他的物理和数学计算以及工程发展,光合作用研究取得了主要成就。谢杨·赫兹克服困难的能力、坚韧不拔的精神、活力和乐观精神得到了人们的注意。
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引用次数: 0
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