Nihon juigaku zasshi. The Japanese journal of veterinary science最新文献

Ultraviolet (UV)-irradiation of peripheral blood lymphocytes (PBL) of miniature swine prevented them from initiating proliferative responses in allogeneic mixed lymphocyte reactions (MLR). When pigs were given 4 weekly intravenous transfusions of UV-irradiated allogeneic donor PBL differing in major histocompatibility (MHC), PBL of recipient pigs progressively responded less vigorously to donor PBL in MLRs over the treatment period. These pigs did not produce anti-donor PBL antibody. Pigs treated with UV-irradiated PBL also had negligible delayed type hypersensitivity (DTH) responses to donor PBL at the end of the treatment period. In contrast, pigs receiving injections of untreated allogeneic PBL gave strong DTH responses to donor PBL, high proliferation in MLRs with donor PBL and all produced anti-donor PBL antibody.

Isolation of Cryptococcus neoformans was carried out on sunflower seed agar medium (SFA) and Sabouraud dextrose agar (SDA). Out of 346 environmental substrates (133 fruits, 107 avian extreta, 91 vegetables and 15 wooden scrapings) tested, 3 specimens were positive for C. neoformans. The positive isolations came from the fruits of 2 banana (Musa sapientum) and a potato tuber (Solnum tuberosum). The pathogen could not be demonstrated in 107 samples of avian droppings and 15 of wooden materials. All the 3 isolates of the yeast were obtained on SFA, while they were not cultured on the plates of SDA with chloramphenicol which were badly contaminated with rapidly growing molds, yeasts and bacteria. To the present author's knowledge, this appears to be the first reports of the isolation of this pathogenic basidiomycetous yeast from contaminated fruits of banana. We suggest more comprehensive ecological surveys to search for environmental niche of C. neoformans var. neoformans and C. neoformans var. gattii as the latter variety is also implicated in the etiology of cryptococcosis.

Analysis of surface marker of cells after intratumor injection with Nocardia rubra cell wall skeleton (N-CWS) resulted in gradually increasing percentage of macrophage, Pan T and BoCD4+ cells. Proportion of BoCD8+ cells gradually increased from 4th day and then decreased from 8th day after the injection. Fresh tumor infiltrated cells obtained from lymphatic nodule at 8 days after injection of N-CWS showed cytotoxic activity against bovine leukemia cell line, but this activity decreased with the time of cultivation and no activity could be detected after 14 days cultivation. These cultured cells were injected twice to lymphatic nodule at one week interval for adoptive immunotherapy and found to induce complete regression of nodule after 5 weeks from first injection.

Eighteen canine herpesvirus (CHV) isolates from Japan and two reference strains were compared by restriction endonuclease analysis technique using total DNA extracts from cells infected with the viruses. In order to select the suitable restriction endonucleases for differentiation of CHV isolates, ten enzymes were used and three of them, HindIII, XbaI, and PvuII, were found to be useful for strain differentiation. With these enzymes, CHV isolates from unrelated individuals were readily differentiated from each other. In contrast, all the isolates derived from the same litter were not distinguishable on the basis of restriction cleavage patterns. However, slight mobility shifts were observed among the isolates from the same litter or the same individual. The results showed that this method provides a powerful tool for epidemiological surveys of CHV infection.