Today, the use of mesenchymal stem cells (MSCs) for treating human diseases has attracted wide attention. The aim of this study is the expression of dopaminergic genes such as Nestin, patched Tumor Suppressor (PTCH), Sonic Hedgehog (SHH), Tyrosine Hydroxylase (TH) and Nuclear receptor-related factor 1 (NURR1) in MSCs after induction with selegiline. Rat bone marrow mesenchymal stem cells (rBMSCs) were extracted from femur and tibia bones and incubated with alpha Minimum Essential Medium (α-MEM) and 10% Fetal bovine serum (FBS). The stemness of cells at passage 4 was determined by the positive response to CD71 and CD90 markers and their differentiation into adipocytes and osteoblasts. The expression of SHH, PTCH, TH, NURR1 and Nestin genes in the cells after induction by 10-8 M selegiline for 48 hours was investigated by Reverse transcription polymerase chain reaction (RT-PCR) and Real Time-PCR methods. Isolated rBMSCs expressed CD71 and CD90 markers in culture conditions and could differentiate into adipocytes and osteoblasts. Induced cells showed neuronal morphology, positive response to Nestin and TH immunostaining. There was a significant increase of dopaminergic genes TH and NURR1 compared to the untreated cells. The results showed that selegiline with a dose of 10-8 M for 48 hours can lead to dopaminergic differentiation in rBMSCs.
{"title":"Selegiline induced differentiation of rat bone marrow mesenchymal stem cells to dopaminergic neurons in vitro","authors":"Dhiya Altememy, Maryam Haji Ghasem Kashani, Pegah Khosravian","doi":"10.3897/pharmacia.70.e107909","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e107909","url":null,"abstract":"Today, the use of mesenchymal stem cells (MSCs) for treating human diseases has attracted wide attention. The aim of this study is the expression of dopaminergic genes such as Nestin, patched Tumor Suppressor (PTCH), Sonic Hedgehog (SHH), Tyrosine Hydroxylase (TH) and Nuclear receptor-related factor 1 (NURR1) in MSCs after induction with selegiline. Rat bone marrow mesenchymal stem cells (rBMSCs) were extracted from femur and tibia bones and incubated with alpha Minimum Essential Medium (α-MEM) and 10% Fetal bovine serum (FBS). The stemness of cells at passage 4 was determined by the positive response to CD71 and CD90 markers and their differentiation into adipocytes and osteoblasts. The expression of SHH, PTCH, TH, NURR1 and Nestin genes in the cells after induction by 10-8 M selegiline for 48 hours was investigated by Reverse transcription polymerase chain reaction (RT-PCR) and Real Time-PCR methods. Isolated rBMSCs expressed CD71 and CD90 markers in culture conditions and could differentiate into adipocytes and osteoblasts. Induced cells showed neuronal morphology, positive response to Nestin and TH immunostaining. There was a significant increase of dopaminergic genes TH and NURR1 compared to the untreated cells. The results showed that selegiline with a dose of 10-8 M for 48 hours can lead to dopaminergic differentiation in rBMSCs.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135596830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current study, we investigated the effect of creatine monohydrate (CrM) and newly synthesized creatine lysinate (CrLys) in tail suspension (TST) and rotarod tests and their influence on the histology of the skeletal muscles. In the TST, a slight decrease in the immobility time from the 1 st to the 3 rd week was observed in the group treated with CrM at a dose of 1.5 g/kg/day and CrLys at a dose of 6 g/kg/day. The rotarod test revealed that CrM (1.5 g/kg/day) and CrLys (3 g/kg/day) lead to a significant improvement in motor coordination in the 3 rd week. The results from histology showed an increase in the muscle fiber diameter of soleus muscle in animals treated with CrM (3 g/kg/day) and CrLys (6 g/kg/day). The results showed that supplementation with creatine derivatives appears to be a generally effective nutritional ergogenic aid for an improvement of physical performance.
{"title":"Creatine lysinate – part II: effects on the motor coordination and muscle hypertrophy in mice","authors":"Ivanka Kostadinova, Nikolai Danchev, Boycho Landzhov, Dobrina Tsvetkova, Lyubomir Marinov, Ivalina Ivanova","doi":"10.3897/pharmacia.70.e110718","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110718","url":null,"abstract":"In the current study, we investigated the effect of creatine monohydrate (CrM) and newly synthesized creatine lysinate (CrLys) in tail suspension (TST) and rotarod tests and their influence on the histology of the skeletal muscles. In the TST, a slight decrease in the immobility time from the 1 st to the 3 rd week was observed in the group treated with CrM at a dose of 1.5 g/kg/day and CrLys at a dose of 6 g/kg/day. The rotarod test revealed that CrM (1.5 g/kg/day) and CrLys (3 g/kg/day) lead to a significant improvement in motor coordination in the 3 rd week. The results from histology showed an increase in the muscle fiber diameter of soleus muscle in animals treated with CrM (3 g/kg/day) and CrLys (6 g/kg/day). The results showed that supplementation with creatine derivatives appears to be a generally effective nutritional ergogenic aid for an improvement of physical performance.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135591124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Individual NAT2 genotype identity data should be enriched to prevent Isoniazid-induced liver injury (IDILI) and optimize the dose of Isoniazid (INH). Therefore, this study aims to present the level of IDILI risk for specific genotype alleles. The data collection involves literature indexed by Google Scholar, Scopus, and Pubmed databases. The search uses a combination of the following keyword variants “INH” OR “INH”, “liver injury” OR “hepatotoxicity”, “polymorphism” OR “pharmacogenomic”, and “N-acetyltransferase 2” OR “NAT2”. Furthermore, the screening results of library sources were narrowed to 11 original articles that met the inclusion criteria. The IDILI risk assessment analysis due to NAT2 enzyme polymorphism following the odds ratio has a 95% confidence interval. The results showed that the IDILI risk level of the slow acetylator group was 3.11 times higher than other populations. Meanwhile, the rapid and intermediate acetylator groups were not at risk. Three variants related to *6 allele were classified as high risk; *6A/*6A risk 5.76 times, *6A/*7B (5.54 times), and *6/*7 (4 times). The three allele configurations of the *5 and *7 were also classified as a risk; *5B/*7B (5 times), *7B/*7B (3.23 times), and *5/*7 (2,74 times).
为预防异烟肼所致肝损伤(IDILI)和优化异烟肼(INH)剂量，应丰富个体NAT2基因型识别数据。因此，本研究旨在了解特定基因型等位基因的IDILI风险水平。数据收集涉及b谷歌Scholar、Scopus和Pubmed数据库索引的文献。搜索使用以下关键词变体“INH”或“INH”，“肝损伤”或“肝毒性”，“多态性”或“药物基因组学”，以及“n -乙酰转移酶2”或“NAT2”的组合。此外，图书馆来源的筛选结果被缩小到11篇符合纳入标准的原创文章。基于NAT2酶多态性的IDILI风险评估分析的优势比为95%置信区间。结果显示，慢乙酰化组的IDILI风险水平是其他人群的3.11倍。快速和中间乙酰化剂组无危险。*6等位基因相关的3个变异被归为高危;*6A/*6A风险为5.76倍，*6A/*7B风险为5.54倍，*6/*7风险为4倍。*5和*7的3个等位基因配置也被归为风险;* 5 b / * 7 b(5次),* b / 7 * 7 b(3.23倍),和* 5 / * 7(74次)。
{"title":"Isoniazid-induced liver injury risk level in different variants of N-acetyltransferase 2 (NAT2) polymorphisms: A literature review","authors":"Setiyo Budi Santoso, Prasojo Pribadi, Lalu Muhammad Irham","doi":"10.3897/pharmacia.70.e109869","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e109869","url":null,"abstract":"Individual NAT2 genotype identity data should be enriched to prevent Isoniazid-induced liver injury (IDILI) and optimize the dose of Isoniazid (INH). Therefore, this study aims to present the level of IDILI risk for specific genotype alleles. The data collection involves literature indexed by Google Scholar, Scopus, and Pubmed databases. The search uses a combination of the following keyword variants “INH” OR “INH”, “liver injury” OR “hepatotoxicity”, “polymorphism” OR “pharmacogenomic”, and “N-acetyltransferase 2” OR “NAT2”. Furthermore, the screening results of library sources were narrowed to 11 original articles that met the inclusion criteria. The IDILI risk assessment analysis due to NAT2 enzyme polymorphism following the odds ratio has a 95% confidence interval. The results showed that the IDILI risk level of the slow acetylator group was 3.11 times higher than other populations. Meanwhile, the rapid and intermediate acetylator groups were not at risk. Three variants related to *6 allele were classified as high risk; *6A/*6A risk 5.76 times, *6A/*7B (5.54 times), and *6/*7 (4 times). The three allele configurations of the *5 and *7 were also classified as a risk; *5B/*7B (5 times), *7B/*7B (3.23 times), and *5/*7 (2,74 times).","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135590790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to assess the hypoglycemic effects of Ngu-Vi-Tieu-Khat (NVTK) decoction, a traditional Vietnamese medicine, in a rat model of type-2 diabetes. The NVTK decoction was prepared using the maceration method and tested for its hypoglycemic effects by measuring blood glucose levels, insulin resistance indicators, and pancreatic mass. The results showed that NVTK decoction improved diabetes symptoms, increased insulin levels, reduced insulin resistance, restored pancreatic mass, and decreased total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) while increasing high-density lipoprotein cholesterol (HDL-C). The hypoglycemic effects of NVTK were comparable to those of gliclazide at a dose of 10 mg/kg. In conclusion, NVTK decoction possesses hypoglycemic properties and could be explored as a potential traditional medicine for treating type-2 diabetes in humans.
本研究旨在评估越南传统药物ngu - vi - tieuu - khat (NVTK)汤对2型糖尿病大鼠模型的降糖作用。采用浸渍法制备NVTK煎剂，通过测定血糖水平、胰岛素抵抗指标和胰腺质量来检测其降血糖作用。结果显示，NVTK汤改善糖尿病症状，提高胰岛素水平，降低胰岛素抵抗，恢复胰腺质量，降低总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)，升高高密度脂蛋白胆固醇(HDL-C)。NVTK的降糖作用与剂量为10mg /kg的格列齐特相当。综上所述，NVTK汤具有降糖作用，可作为治疗人类2型糖尿病的潜在传统药物。
{"title":"Ngu-Vi-Tieu-Khat decoction, a Vietnamese traditional medicine, possesses hypoglycemic and hypolipidemic effects on streptozotocin-induced type-2 diabetic rat model","authors":"Minh Hoang Le, Huyen Sanh Sam, Duy Toan Pham, Ngoc Chi Lan Nguyen, Ngoc Diem Le, Tran Nhat Phong Dao","doi":"10.3897/pharmacia.70.e108879","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e108879","url":null,"abstract":"This study aims to assess the hypoglycemic effects of Ngu-Vi-Tieu-Khat (NVTK) decoction, a traditional Vietnamese medicine, in a rat model of type-2 diabetes. The NVTK decoction was prepared using the maceration method and tested for its hypoglycemic effects by measuring blood glucose levels, insulin resistance indicators, and pancreatic mass. The results showed that NVTK decoction improved diabetes symptoms, increased insulin levels, reduced insulin resistance, restored pancreatic mass, and decreased total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) while increasing high-density lipoprotein cholesterol (HDL-C). The hypoglycemic effects of NVTK were comparable to those of gliclazide at a dose of 10 mg/kg. In conclusion, NVTK decoction possesses hypoglycemic properties and could be explored as a potential traditional medicine for treating type-2 diabetes in humans.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135696033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Headaches, especially migraines and tension-type headaches (TTH), are among the most prevalent problems encountered in neurology and medicine. Ongoing debate surrounds the pathophysiological mechanisms behind this condition. One of the potential ideas involves inflammation and diet. The purpose of the study was to compare the serum levels of C-reactive protein (CRP), D-dimer, vitamin D and magnesium in 23 migraineurs and 23 TTH-diagnosed sufferers. The CRP level (4.8±3.7 mg/L) was found to be significantly different between migraineurs and TTH sufferers, with a significant difference (p=0.03) between the two groups of headache sufferers. Vitamin D serum levels (19.5±7.9 ng/L) and TTH serum levels (26.7±8.4) were observed to differ significantly. Magnesium serum levels (p=0.692) did not differ significantly between the two headache patient groups.
{"title":"Comparison of C-reactive protein, D-dimer, vitamin D and magnesium serum levels between migraineurs and tension-type headache sufferers","authors":"Khairul Putra Surbakti, Eka Mahendrayana, Chairil Amin Batubara","doi":"10.3897/pharmacia.70.e103131","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e103131","url":null,"abstract":"Headaches, especially migraines and tension-type headaches (TTH), are among the most prevalent problems encountered in neurology and medicine. Ongoing debate surrounds the pathophysiological mechanisms behind this condition. One of the potential ideas involves inflammation and diet. The purpose of the study was to compare the serum levels of C-reactive protein (CRP), D-dimer, vitamin D and magnesium in 23 migraineurs and 23 TTH-diagnosed sufferers. The CRP level (4.8±3.7 mg/L) was found to be significantly different between migraineurs and TTH sufferers, with a significant difference (p=0.03) between the two groups of headache sufferers. Vitamin D serum levels (19.5±7.9 ng/L) and TTH serum levels (26.7±8.4) were observed to differ significantly. Magnesium serum levels (p=0.692) did not differ significantly between the two headache patient groups.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The colorimetric paper-based device using lipase inhibition assay was developed for rapid and visual detection of orlistat in weight loss supplements. The paper device with five circular detection zones was simply fabricated from filter paper No 1 using a low-cost paper craft puncher with a design of a flower-like shape. The enzymatic reaction on the detection zone employed a small volume of a substrate, a-naphthyl acetate, a sample, and a lipase enzyme. After incubation, the Fast Blue B solution was used as a chromogenic reagent. The decrease in purple color can be observed by the naked eye, in the presence of orlistat. Under optimized conditions, the paper device showed satisfactory sensitivity and selectivity. The device was applied for rapid screening of orlistat in weight loss supplements and the results agreed with those obtained from TLC analysis.
采用脂肪酶抑制法的比色纸装置，用于快速和视觉检测减肥补充剂中的奥利司他。具有五个圆形检测区域的纸张装置是用低成本的纸张工艺打孔机以1号滤纸为原料制作而成的，打孔机的设计呈花朵状。检测区的酶促反应采用了少量底物、乙酸a-萘酯、样品和脂肪酶。孵育后，使用Fast Blue B溶液作为显色试剂。在奥利司他存在的情况下，可以用肉眼观察到紫色的减少。在优化条件下，该装置具有良好的灵敏度和选择性。将该装置用于减肥补充剂中奥利司他的快速筛选，结果与薄层色谱分析结果一致。
{"title":"Colorimetric paper-based device for rapid screening of orlistat in weight loss supplements","authors":"Panadda Phattanawasin, Theerasak Rojanarata, Nusara Piyapolrungroj","doi":"10.3897/pharmacia.70.e108150","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e108150","url":null,"abstract":"The colorimetric paper-based device using lipase inhibition assay was developed for rapid and visual detection of orlistat in weight loss supplements. The paper device with five circular detection zones was simply fabricated from filter paper No 1 using a low-cost paper craft puncher with a design of a flower-like shape. The enzymatic reaction on the detection zone employed a small volume of a substrate, a-naphthyl acetate, a sample, and a lipase enzyme. After incubation, the Fast Blue B solution was used as a chromogenic reagent. The decrease in purple color can be observed by the naked eye, in the presence of orlistat. Under optimized conditions, the paper device showed satisfactory sensitivity and selectivity. The device was applied for rapid screening of orlistat in weight loss supplements and the results agreed with those obtained from TLC analysis.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135738560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Burnout is described as an emotional, mental and physical exhaustion observed in healthy people and directly related to their professional context. The objective is to study the level of burnout among community pharmacists and to identify the workplace factors affecting it. Materials and methods : Using a questionnaire, specifically designed by the authors of the study, an anonymous survey was conducted in the region of Varna, as the largest region of Northeastern Bulgaria. The questionnaire contains questions about the demographic characteristics; a standardised burnout measure tool (MBI-HSS-MP), and questions regarding the specifics of the work environment. According to MBI-HSS-MP, burnout is associated with high levels of emotional exhaustion (EE) and depersonalisation (DP) and low level of personal accomplishment (PA). Results : The participants in the pilot study were 127 masters of pharmacy. Regarding individual factors, a statistically significant relationship was found between age and the scales emotional exhaustion EE (p = 0.012), depersonalisation DP (0.028) and personal accomplishment PA (p = 0.028). Most susceptible to ЕЕ (p = 0.014) and DP (p = 0.008) were the age group 25–45 years while most satisfied with their PA were in the age group 46–55 years (p = 0.016). From the organisational factors, the large number of patients had the greatest impact on burnout (DP р = 0.000 /ЕЕ р = 0.006). The other key factors for burnout were an insufficient number of pharmacy staff, a heavy administrative burden and a wide range of medicinal products. Conclusion : It is necessary to develop training courses for young professionals to introduce them gradually into the work process and build up skills for effectively coping with stress. Proposals need to be made for reducing the administrative burden of pharmacists and maintaining staffing levels that are adequate to the patient flow and expanding the capabilities of The Specialised Electronic System for Tracing and Analysis System of Medicinal Products (SETASMP).
{"title":"Prevalence and risk factors of burnout among community pharmacists in Northeast region of Bulgaria - a pilot study","authors":"Mariya Ivanova, Anna Todorova, Lora Georgieva","doi":"10.3897/pharmacia.70.e110145","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110145","url":null,"abstract":"Background : Burnout is described as an emotional, mental and physical exhaustion observed in healthy people and directly related to their professional context. The objective is to study the level of burnout among community pharmacists and to identify the workplace factors affecting it. Materials and methods : Using a questionnaire, specifically designed by the authors of the study, an anonymous survey was conducted in the region of Varna, as the largest region of Northeastern Bulgaria. The questionnaire contains questions about the demographic characteristics; a standardised burnout measure tool (MBI-HSS-MP), and questions regarding the specifics of the work environment. According to MBI-HSS-MP, burnout is associated with high levels of emotional exhaustion (EE) and depersonalisation (DP) and low level of personal accomplishment (PA). Results : The participants in the pilot study were 127 masters of pharmacy. Regarding individual factors, a statistically significant relationship was found between age and the scales emotional exhaustion EE (p = 0.012), depersonalisation DP (0.028) and personal accomplishment PA (p = 0.028). Most susceptible to ЕЕ (p = 0.014) and DP (p = 0.008) were the age group 25–45 years while most satisfied with their PA were in the age group 46–55 years (p = 0.016). From the organisational factors, the large number of patients had the greatest impact on burnout (DP р = 0.000 /ЕЕ р = 0.006). The other key factors for burnout were an insufficient number of pharmacy staff, a heavy administrative burden and a wide range of medicinal products. Conclusion : It is necessary to develop training courses for young professionals to introduce them gradually into the work process and build up skills for effectively coping with stress. Proposals need to be made for reducing the administrative burden of pharmacists and maintaining staffing levels that are adequate to the patient flow and expanding the capabilities of The Specialised Electronic System for Tracing and Analysis System of Medicinal Products (SETASMP).","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135697317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives : To quantify quercetin, gallic acid, rutin, naringin, and caffeic acid in the rhizome of Zingiber officinale different extracts in seven different solvents (methanol, ethanol, ethyl acetate, water, dichloromethane, chloroform, and n -hexane), for the first time, using HPLC/UV. Also, to study the anticancer activity of Zingiber officinale different extracts by evaluating its in vitro toxicity on HT-29 colorectal cancer cell line. Methods : The fresh and dried rhizomes were extracted using Soxhlet (SOX) and maceration (MAC) methods. Separation of compounds was conducted using HPLC. The cell line used for MTT cell proliferation assay antiproliferative; is HT-29 (HTB-38) colorectal adenocarcinoma. Results : The MTT test indicated that powder ginger extracted by MAC or SOX showed high cytotoxicity activity (IC 50 <50) against HT-29 cells, except water using SOX, which showed mild cytotoxicity activity. The fresh ginger extracted by MAC using dichloromethane and those extracted by SOX using ethyl acetate showed strong cytotoxicity activity (IC 50 <50). Conclusion : The phenolic and flavonoid contents of ginger can vary depending on the different extracts from ginger plant. Also, HPLC results revealed that quercetin was the highest in all extracts.
{"title":"Quantitative analysis of total phenolic and flavonoid compounds in different extracts from ginger plant (Zingiber officinale) and evaluation of their anticancer effect against colorectal cancer cell lines","authors":"Nadeen Waleed Al-Areer, Khaldun M. Al Azzam, Rima H. Al Omari, Ibrahim Al-Deeb, Lyazzat Bekbayeva, El-Sayed Negim","doi":"10.3897/pharmacia.70.e103936","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e103936","url":null,"abstract":"Objectives : To quantify quercetin, gallic acid, rutin, naringin, and caffeic acid in the rhizome of Zingiber officinale different extracts in seven different solvents (methanol, ethanol, ethyl acetate, water, dichloromethane, chloroform, and n -hexane), for the first time, using HPLC/UV. Also, to study the anticancer activity of Zingiber officinale different extracts by evaluating its in vitro toxicity on HT-29 colorectal cancer cell line. Methods : The fresh and dried rhizomes were extracted using Soxhlet (SOX) and maceration (MAC) methods. Separation of compounds was conducted using HPLC. The cell line used for MTT cell proliferation assay antiproliferative; is HT-29 (HTB-38) colorectal adenocarcinoma. Results : The MTT test indicated that powder ginger extracted by MAC or SOX showed high cytotoxicity activity (IC 50 &lt;50) against HT-29 cells, except water using SOX, which showed mild cytotoxicity activity. The fresh ginger extracted by MAC using dichloromethane and those extracted by SOX using ethyl acetate showed strong cytotoxicity activity (IC 50 &lt;50). Conclusion : The phenolic and flavonoid contents of ginger can vary depending on the different extracts from ginger plant. Also, HPLC results revealed that quercetin was the highest in all extracts.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135828914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Atrial fibrillation (AF) is the most common heart rhythm disorder. AF ablation based on endovascular or surgical techniques is a standard of care in everyday practice. Early recurrence of atrial arrhythmias is a significant problem after ablative procedures. Prophylactic use of antiarrhythmic drugs has become a possible solution. Materials and methods : We performed a retrospective observational cohort study of 59 patients with heart disease requiring surgery under extracorporeal circulation (ECC) and have received radiofrequency ablation (RFA) for chronic or persistent AF with a successful conversion to sinus rhythm. Patients were divided into two groups: 36 were treated with amiodarone for three months postoperatively (treatment group), and 23 had no concomitant amiodarone therapy (control group). We compared the groups regarding freedom from recurrent atrial arrhythmia and the frequency of conduction disorders requiring permanent pacemaker implantation. Results : No differences between the groups were found regarding demographic characteristics, risk profile, and heart diseases indicating cardiac surgery. During follow-up, a significantly higher proportion of patients with sinus rhythm in the group receiving amiodarone was found (80.56% vs. 52.17%; p < 0.041). No cases of premature discontinuation of amiodarone for side effects or noncompliance were registered in the treatment group. Conclusion : Three months of amiodarone therapy post-surgical RFA of AF is an effective and safe strategy for relapse prevention.
背景:心房颤动(AF)是最常见的心律失常。基于血管内或外科技术的房颤消融是日常实践中的标准护理。心房心律失常的早期复发是消融手术后的一个重要问题。预防性使用抗心律失常药物已成为一种可能的解决方案。材料和方法:我们对59例需要体外循环(ECC)手术的心脏病患者进行了回顾性观察队列研究，这些患者接受了射频消融(RFA)治疗慢性或持续性房颤，并成功转化为窦性心律。将患者分为两组:36例患者术后3个月接受胺碘酮治疗(治疗组)，23例患者未同时接受胺碘酮治疗(对照组)。我们比较了两组复发性心房心律失常和需要植入永久性起搏器的传导障碍的频率。结果:在人口统计学特征、风险概况和需要进行心脏手术的心脏病方面，各组之间没有发现差异。随访中，胺碘酮组出现窦性心律的比例明显高于对照组(80.56% vs. 52.17%;p, lt;0.041)。治疗组没有因副作用或不依从性而过早停药的病例。结论:房颤RFA术后3个月的胺碘酮治疗是一种安全有效的预防房颤复发策略。
{"title":"Amiodarone prevention for atrial fibrillation relapse after surgical ablation","authors":"Ivayla Zheleva-Kyuchukova, Dimitar Kyuchukov","doi":"10.3897/pharmacia.70.e110256","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110256","url":null,"abstract":"Background : Atrial fibrillation (AF) is the most common heart rhythm disorder. AF ablation based on endovascular or surgical techniques is a standard of care in everyday practice. Early recurrence of atrial arrhythmias is a significant problem after ablative procedures. Prophylactic use of antiarrhythmic drugs has become a possible solution. Materials and methods : We performed a retrospective observational cohort study of 59 patients with heart disease requiring surgery under extracorporeal circulation (ECC) and have received radiofrequency ablation (RFA) for chronic or persistent AF with a successful conversion to sinus rhythm. Patients were divided into two groups: 36 were treated with amiodarone for three months postoperatively (treatment group), and 23 had no concomitant amiodarone therapy (control group). We compared the groups regarding freedom from recurrent atrial arrhythmia and the frequency of conduction disorders requiring permanent pacemaker implantation. Results : No differences between the groups were found regarding demographic characteristics, risk profile, and heart diseases indicating cardiac surgery. During follow-up, a significantly higher proportion of patients with sinus rhythm in the group receiving amiodarone was found (80.56% vs. 52.17%; p &lt; 0.041). No cases of premature discontinuation of amiodarone for side effects or noncompliance were registered in the treatment group. Conclusion : Three months of amiodarone therapy post-surgical RFA of AF is an effective and safe strategy for relapse prevention.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135425154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This scoping review aimed to examine the existing evidence on complementary and alternative medicine interventions for preeclampsia, emphasizing empowering midwives in postpartum treatment. Three databases (PubMed, Web of Science, Scopus) were used following the PRISMA-2020 guidelines to find articles from January 2012 to December 2022 and synthesized using Rayyan AI software. A total of 707 articles were initially identified, 7 were included in the review. Traditional Chinese Medicine combined with Western medicine showed promise in treating severe preeclampsia. Massage therapy reduced anxiety, aromatherapy positively affected postpartum well-being, and warm foot therapy reduced swelling associated with postpartum preeclampsia. Using herbal ingredients, lavender aromatherapy, magnesium sulfate, massage, and physical therapy contributed to successful postpartum preeclampsia management. Complementary and alternative medicine, collaboration with community health workers, clear guidelines, targeted training, and future research are crucial for effective postpartum preeclampsia management.
本综述旨在研究补充和替代药物干预子痫前期的现有证据，强调助产士在产后治疗中的权力。根据PRISMA-2020指南使用PubMed、Web of Science、Scopus三个数据库查找2012年1月至2022年12月的文章，并使用Rayyan人工智能软件进行合成。初步确定共707篇，其中7篇纳入综述。中西医结合治疗重度子痫前期疗效显著。按摩疗法减少焦虑，芳香疗法积极影响产后健康，暖足疗法减少与产后子痫前期相关的肿胀。使用草药成分，薰衣草芳香疗法，硫酸镁，按摩和物理治疗有助于成功产后子痫前期管理。补充和替代医学、与社区卫生工作者合作、明确的指导方针、有针对性的培训和未来的研究对于有效的产后子痫前期管理至关重要。
{"title":"Complementary and alternatives care for postpartum preeclampsia: A scoping review","authors":"Susilo Rini, Soetrisno Soetrisno, Tri Rejeki Andayani, Suminah Suminah","doi":"10.3897/pharmacia.70.e110572","DOIUrl":"https://doi.org/10.3897/pharmacia.70.e110572","url":null,"abstract":"This scoping review aimed to examine the existing evidence on complementary and alternative medicine interventions for preeclampsia, emphasizing empowering midwives in postpartum treatment. Three databases (PubMed, Web of Science, Scopus) were used following the PRISMA-2020 guidelines to find articles from January 2012 to December 2022 and synthesized using Rayyan AI software. A total of 707 articles were initially identified, 7 were included in the review. Traditional Chinese Medicine combined with Western medicine showed promise in treating severe preeclampsia. Massage therapy reduced anxiety, aromatherapy positively affected postpartum well-being, and warm foot therapy reduced swelling associated with postpartum preeclampsia. Using herbal ingredients, lavender aromatherapy, magnesium sulfate, massage, and physical therapy contributed to successful postpartum preeclampsia management. Complementary and alternative medicine, collaboration with community health workers, clear guidelines, targeted training, and future research are crucial for effective postpartum preeclampsia management.","PeriodicalId":20086,"journal":{"name":"Pharmacia","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135387360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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