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Analysis of transcriptomic profiles and physiological traits of exogenous 24-epibrassinolide alleviating salt stress in Atractylodes macrocephala Koidz. 外源24-表油菜素内酯缓解白术盐胁迫的转录组学特征及生理特性分析。

IF 2.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Reports

Pub Date : 2023-11-15 DOI: 10.1007/s11816-023-00874-1

Jianyun Zhang, Huiyong Fang, Jianming Jiang, Xian Gu, Huimei Zhao, Jinmiao Qiu, Qian Wang, Yuguang Zheng, Kaiyan Zheng

Exogenous 24-epibrassinolide (24-epiBL) application is a preferred method for improving salt tolerance in plants. However, information about the effects of exogenous 24-epiBL application related to salt response in Atractylodes macrocephala Koidz. (AMK) has rarely been reported. Here, we aimed to investigate the effects of exogenous 24-epiBL (0.01 mg L⁻¹) on enhancing salt tolerance in AMK seedlings under salt stress (5 g L⁻¹ NaCl) based on seed germination experiments. Our results indicated that NaCl treatment suppresses AMK seed germination, while 24-epiBL treatment under nonstress and stress conditions had positive effects on plant seedling growth. Treatment with NaCl significantly inhibited radicle elongation and root activity to endure salt stress, and 24-epiBL treatment promoted growth and alleviated the damage to osmotic stress, ion stress substances, and oxidative stress by regulating free proline (Pro) and soluble protein (SP) contents and enhancing soluble sugar (SS) conversion. Moreover, the expression of normal development process genes was affected, and differentially expressed genes (DEGs) in multiple pathways associated with amino acid metabolism, signal transduction, carbohydrate metabolism, and lipid metabolism were regulated under NaCl treatment. Further analysis of the AMK transcriptome profile revealed that exogenous 24-epiBL enhanced salt tolerance through hormone regulation and carbohydrate metabolism promotion, salt-responsive gene expression inhibition, and induction of pathways related to terpenoid and polyketide metabolism as well as pentose and glucuronate interconversions for plant growth promotion. Collectively, our findings successfully demonstrate that exogenous 24-epiBL plays a positive role in regulating the AMK response to salt stress, which provides potential genetic resources to improve salt tolerance in plants.

外源施用24-表油菜素内酯(24-epiBL)是提高植物耐盐性的首选方法。然而，有关外源24-epiBL施用对白术盐反应的影响的信息。(AMK)很少被报道。本研究旨在通过种子萌发试验，研究外源24-epiBL (0.01 mg L−1)对盐胁迫(5 g L−1 NaCl)下AMK幼苗耐盐性的影响。结果表明，NaCl处理抑制AMK种子萌发，而24-epiBL处理在非胁迫和胁迫条件下对植物幼苗生长均有积极影响。NaCl处理显著抑制根伸长和根系活性以抵御盐胁迫，24-epiBL处理通过调节游离脯氨酸(Pro)和可溶性蛋白(SP)含量，提高可溶性糖(SS)转化，促进生长，减轻渗透胁迫、离子胁迫物质和氧化胁迫的损害。此外，NaCl处理影响了正常发育过程基因的表达，并调控了氨基酸代谢、信号转导、碳水化合物代谢和脂质代谢等多种途径的差异表达基因(DEGs)。对AMK转录组谱的进一步分析表明，外源24-epiBL通过激素调控和碳水化合物代谢促进、盐响应基因表达抑制、诱导萜类和聚酮代谢以及戊糖和葡萄糖酸盐相互转化促进植物生长等途径增强耐盐性。综上所述，我们的研究结果成功地证明了外源24-epiBL在调控AMK对盐胁迫的反应中起着积极的作用，这为提高植物的耐盐性提供了潜在的遗传资源。

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