Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.11129
Juan Zhao, Yan Chen, Sheng-Ming Hui, Wei Tu, Chen Chen, Ju Gao
{"title":"Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation","authors":"Juan Zhao, Yan Chen, Sheng-Ming Hui, Wei Tu, Chen Chen, Ju Gao","doi":"10.3724/SP.J.1143.2012.11129","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.11129","url":null,"abstract":"","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"15 1","pages":"164"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78093557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.11162
Ying-hui Duan, Chang Li, Yi Dai, X. Yao
A new phenylpropanediol, (2S)-3, 3-di-(4-hydroxy-3-methoxyphenyl)-propane-1, 2-diol (1), along with a known compound, shizukanolide E (2) were isolated from the water extract of the whole plants of Sarcandra glabra. The structure was elucidated on the basis of extensive NMR, MS, and ORD spectral analysis.
{"title":"A New Phenylpropanediol from Sarcandra glabra (Chloranthaceae)","authors":"Ying-hui Duan, Chang Li, Yi Dai, X. Yao","doi":"10.3724/SP.J.1143.2012.11162","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.11162","url":null,"abstract":"A new phenylpropanediol, (2S)-3, 3-di-(4-hydroxy-3-methoxyphenyl)-propane-1, 2-diol (1), along with a known compound, shizukanolide E (2) were isolated from the water extract of the whole plants of Sarcandra glabra. The structure was elucidated on the basis of extensive NMR, MS, and ORD spectral analysis.","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"1 1","pages":"208"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90096283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.12024
Yi Wang, Qiu-mei Quan, Yun-xiang Li
The effect of flowering time on nectar secretion,effective pollinators activities and fruit set in Epimedium wushanense were revealed by One-way ANOVA.Among the three stage of flowering,the differences in nectar standing crop and sugar content of E.wushanense were very remarkable.In this three populations,the nectar standing crop in the initial stage was higher than that in other stages,but the sugar content of nectar was higher in the last stage.The results indicated that the activities of effective pollinators were influenced by the flowering time significantly.During a day,the activities of effective pollinators were mainly concentrated in the noon.At the same time the visiting frequency of effective pollinators in last stage was higher than that in the initial and middle stage.It was also indicated that the fruit set,the seed number and percentage of seed abortion per fruit of E.wushanense were influenced by the flowering time significantly.During the initial and the middle stage of flowering,the fruit set and the seed number per fruit were bigger than that in the last stage of flowering.But the percentage of seed abortion per fruit was higher in the last stage.So,in order to make work more effective,we should choose the fruits of E.wushanense in the initial of flowering stage for experiments and breeding,and pay attention to the flowering regulation in artificial cultivation.
{"title":"Effects of Flowering Period on Nectar Secretion and Fruit Set of Epimedium wushanense(Berberidaceae)","authors":"Yi Wang, Qiu-mei Quan, Yun-xiang Li","doi":"10.3724/SP.J.1143.2012.12024","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12024","url":null,"abstract":"The effect of flowering time on nectar secretion,effective pollinators activities and fruit set in Epimedium wushanense were revealed by One-way ANOVA.Among the three stage of flowering,the differences in nectar standing crop and sugar content of E.wushanense were very remarkable.In this three populations,the nectar standing crop in the initial stage was higher than that in other stages,but the sugar content of nectar was higher in the last stage.The results indicated that the activities of effective pollinators were influenced by the flowering time significantly.During a day,the activities of effective pollinators were mainly concentrated in the noon.At the same time the visiting frequency of effective pollinators in last stage was higher than that in the initial and middle stage.It was also indicated that the fruit set,the seed number and percentage of seed abortion per fruit of E.wushanense were influenced by the flowering time significantly.During the initial and the middle stage of flowering,the fruit set and the seed number per fruit were bigger than that in the last stage of flowering.But the percentage of seed abortion per fruit was higher in the last stage.So,in order to make work more effective,we should choose the fruits of E.wushanense in the initial of flowering stage for experiments and breeding,and pay attention to the flowering regulation in artificial cultivation.","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"34 1","pages":"471"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76169884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.12032
Yuan Lin, Di-qiu Yu
: Several VQ proteins are recently identified as WRKY factors鄄interacting partners in Arabidopsis and in鄄 volved in regulating physiological processes. Searching of genomic databases found that VQ gene family is specific to land plants and these VQ genes encode proteins characteristic of a conserved VQ鄄motif. It consists of 34 representa鄄 tives in Arabidopsis and can be divided into two groups based on the similarity of the amino acid sequences. To un鄄 derstand the functions of Arabidopsis VQ proteins, we examined the expression profiles of AtVQ genes in various de鄄 fense鄄related hormones treatments. qRT鄄PCR analysis revealed that a majority of them were differently regulated in response to salicylic acid (SA), methyl jasmonate (MeJA), or 1鄄aminocyclopropane鄄1鄄carboxylate (ACC). And some members are induced by two of these three hormones. Moreover, four members ( AtVQ3 , AtVQ18 , AtVQ23 and AtVQ24 ) are induced by SA, MeJA and ACC simultaneity. However, there is only one AtVQ gene ( AtVQ27 ) is up鄄 regulated after spraying of abscisic acid (ABA). These results suggest that Arabidopsis VQ genes may be involved in plant defense responses.
{"title":"Expression Profiles of Arabidopsis thaliana VQ Gene Family in Defense-Related Hormones Treatments","authors":"Yuan Lin, Di-qiu Yu","doi":"10.3724/SP.J.1143.2012.12032","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12032","url":null,"abstract":": Several VQ proteins are recently identified as WRKY factors鄄interacting partners in Arabidopsis and in鄄 volved in regulating physiological processes. Searching of genomic databases found that VQ gene family is specific to land plants and these VQ genes encode proteins characteristic of a conserved VQ鄄motif. It consists of 34 representa鄄 tives in Arabidopsis and can be divided into two groups based on the similarity of the amino acid sequences. To un鄄 derstand the functions of Arabidopsis VQ proteins, we examined the expression profiles of AtVQ genes in various de鄄 fense鄄related hormones treatments. qRT鄄PCR analysis revealed that a majority of them were differently regulated in response to salicylic acid (SA), methyl jasmonate (MeJA), or 1鄄aminocyclopropane鄄1鄄carboxylate (ACC). And some members are induced by two of these three hormones. Moreover, four members ( AtVQ3 , AtVQ18 , AtVQ23 and AtVQ24 ) are induced by SA, MeJA and ACC simultaneity. However, there is only one AtVQ gene ( AtVQ27 ) is up鄄 regulated after spraying of abscisic acid (ABA). These results suggest that Arabidopsis VQ genes may be involved in plant defense responses.","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"84 1","pages":"509"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76173737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.12040
C. Hao, L. Tan, R. Fan, Huan Yu, Jian-feng Yang, Hua-song Wu
{"title":"Floristic Geography of Piper (Piperaceae) in China","authors":"C. Hao, L. Tan, R. Fan, Huan Yu, Jian-feng Yang, Hua-song Wu","doi":"10.3724/SP.J.1143.2012.12040","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12040","url":null,"abstract":"","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"20 1","pages":"421"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75649682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.12006
Yan Xie, Hang Sun, Z. Nie
{"title":"Pollen Morphology of Tibetia (Fabaceae) from the Hengduan Mountains, with Emphasis on the Taxonomical Status of Tibetia liangshanensis","authors":"Yan Xie, Hang Sun, Z. Nie","doi":"10.3724/SP.J.1143.2012.12006","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12006","url":null,"abstract":"","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"70 1","pages":"324"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73876994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A 4 231 bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques.The result of alignment and structure prediction of DNA sequences showed that one 1 194 bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found,including four introns(72 bp,49 bp,48 bp and 41 bp) and five extrons(217 bp,113 bp,67 bp,138 bp and 449 bp).The spliced open reading frame(ORF) contains 984 bp nucleotides encoding 327 amino acid residues,and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors.The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors,which suggested multiple ways of evolution occurred in fungi.
{"title":"Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)","authors":"Wei-min Chen, Hongmei Chai, Xiaolei Zhang, SHU-HONG Li, Yongchang Zhao","doi":"10.3724/SP.J.1143.2012.12028","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12028","url":null,"abstract":"A 4 231 bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques.The result of alignment and structure prediction of DNA sequences showed that one 1 194 bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found,including four introns(72 bp,49 bp,48 bp and 41 bp) and five extrons(217 bp,113 bp,67 bp,138 bp and 449 bp).The spliced open reading frame(ORF) contains 984 bp nucleotides encoding 327 amino acid residues,and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors.The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors,which suggested multiple ways of evolution occurred in fungi.","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"11 1","pages":"519"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75080949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.12025
Xiaoling Yan, Shou HaiYang, Ma Jinshuang
{"title":"The problem and status of the alien invasive plants in China.","authors":"Xiaoling Yan, Shou HaiYang, Ma Jinshuang","doi":"10.3724/SP.J.1143.2012.12025","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.12025","url":null,"abstract":"","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"35 1","pages":"287-313"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85505008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.11143
Yong-Gang Ding, W. Chang, Shi-Bao Zhang, Hong Hu
High temperature might be one of the main limiting factors to be introduced and domesticated into lower altitude for Incarvillea zhongdiannensis. To further reveal the underlying molecular mechanism in I.zhongdiannensis responding to high temperature, the suppression subtractive hybridization approach (SSH) was used in this study. The results showed the quality of forward or reverse subtraction cDNA library is good enough for further SSH experiments. Total sixty expressed tags (ESTs) with high quality were randomly obtained by SSH and sequencing. According to BLAST screening and functional annotation, 50 ESTs with known function were classified into 9 broad categories such as defense/stress, photosynthesis, metabolism, energy, signal transduction and transcription, protein fate, transporter, biosynthesis, cell structure and cell growth. There were six ESTs significantly matched with hypothetical proteins. Four ESTs with no hits possibly encode novel genes and play important role in I.zhongdiannensis responding to high temperature stress.
{"title":"Construction of Leave Library by SSH and Preliminary Analysis of Genes Responsible for Heat Sress in Incarvillea zhongdiannensis","authors":"Yong-Gang Ding, W. Chang, Shi-Bao Zhang, Hong Hu","doi":"10.3724/SP.J.1143.2012.11143","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.11143","url":null,"abstract":"High temperature might be one of the main limiting factors to be introduced and domesticated into lower altitude for Incarvillea zhongdiannensis. To further reveal the underlying molecular mechanism in I.zhongdiannensis responding to high temperature, the suppression subtractive hybridization approach (SSH) was used in this study. The results showed the quality of forward or reverse subtraction cDNA library is good enough for further SSH experiments. Total sixty expressed tags (ESTs) with high quality were randomly obtained by SSH and sequencing. According to BLAST screening and functional annotation, 50 ESTs with known function were classified into 9 broad categories such as defense/stress, photosynthesis, metabolism, energy, signal transduction and transcription, protein fate, transporter, biosynthesis, cell structure and cell growth. There were six ESTs significantly matched with hypothetical proteins. Four ESTs with no hits possibly encode novel genes and play important role in I.zhongdiannensis responding to high temperature stress.","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"8 1","pages":"47"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84514063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1143.2012.11185
Yin Xian-Lun, Wang Chun-tao, Kong Xiang-Xiang, Y. Yongping, Hu Xiangyang
Gateway technology is a universal cloning approach that enables rapid cloning of DNA fragments into mul- tiple Gateway-compatible destination vectors using λ phage site-specific recombination , eliminating the requirement to work with restriction enzymes and ligase. But a problem using this system for making entry clone is the expensive- ness and long time to buy the enzyme. To solve this problem , we created the TA cloning entry vector that contained a T-tail in each 3 ' -end through modification of pDONR207. The TA cloning approach can construct entry clones simply , economically and rapidly. Using Gateway T vectors prepared by this improved method , prokaryotic expres- sion vector and eukaryotic expression vector for SOS2 gene were constructed. Through the methods of prokaryotic ex- pression and transient gene expression in Arabidopsis protoplasts , it proved that the SOS2 gene expressed well in both
{"title":"Simplification of Entry Vector by TA Approach","authors":"Yin Xian-Lun, Wang Chun-tao, Kong Xiang-Xiang, Y. Yongping, Hu Xiangyang","doi":"10.3724/SP.J.1143.2012.11185","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2012.11185","url":null,"abstract":"Gateway technology is a universal cloning approach that enables rapid cloning of DNA fragments into mul- tiple Gateway-compatible destination vectors using λ phage site-specific recombination , eliminating the requirement to work with restriction enzymes and ligase. But a problem using this system for making entry clone is the expensive- ness and long time to buy the enzyme. To solve this problem , we created the TA cloning entry vector that contained a T-tail in each 3 ' -end through modification of pDONR207. The TA cloning approach can construct entry clones simply , economically and rapidly. Using Gateway T vectors prepared by this improved method , prokaryotic expres- sion vector and eukaryotic expression vector for SOS2 gene were constructed. Through the methods of prokaryotic ex- pression and transient gene expression in Arabidopsis protoplasts , it proved that the SOS2 gene expressed well in both","PeriodicalId":20246,"journal":{"name":"Plant Diversity and Resources","volume":"43 1","pages":"397"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85776072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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