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Microscopic fungi isolated from different Slovak grape varieties 从斯洛伐克不同葡萄品种中分离的显微真菌
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-17 DOI: 10.5219/893
M. Kačániová, Attila Kántor, M. Terentjeva, Soňa Felšöciová, E. Ivanišová, M. Kluz, P. Hanus, C. Puchalski, M. Horáková
Normal 0 21 false false false EN-GB X-NONE X-NONE The aim of this study was to isolate and identify microscopic fungi in different grape samples. We collected 13 grapes varienties samples (9 white and 4 red) from local Slovak winemakers in the end of the September 2017. Used 13 grape samples in this study: Alibernet, Irsai Oliver, Dornfelder, Blue Frankish, Feteasca regala, Green Veltliner, Palava, Mūller Thurgau, Rhinriesling, Cabernet Savignon, Pinot Blanc, Savignon Blanc and Welschriesling. Microscopic fungi in grape samples were detected on Malt extract agar by spread plate method. The number of microscopic fungi ranged from 2.85 log cfu.g -1 in Cabernet Savignon to 4.83 log cfu.g -1 in Feteasca regala. A total of 627 isolates of microscopic fungi were obtained in this study. The most abundant fungi belonged to genera Alternaria and Penicillium (100% frequency). The high frequency was also detected for Aspergillus (76.92%) and Cladosporium (76.92%) but with lesser relative density. Alternaria sp., Aspergillus niger , Aspergillus sp., Botrytis cinerea , Cladosporium sp., Penicillium expansum , Phoma sp., Rhizopus sp. and Trichoderma sp. species were isolated from grape berries.
本研究的目的是分离和鉴定不同葡萄样品中的显微真菌。2017年9月底,我们从斯洛伐克当地酿酒师那里收集了13个葡萄品种样本(9个白葡萄和4个红葡萄)。本研究使用了13种葡萄样品:艾利伯内、伊尔赛奥利弗、多恩费尔德、蓝弗兰什、费特斯卡雷加、绿维特林纳、帕拉瓦、Mūller图尔高、莱茵司令、赤霞珠萨维农、白皮诺、萨维农和韦氏雷司令。采用涂布平板法在麦芽浸膏琼脂上检测葡萄样品中的显微真菌。显微真菌数量为2.85 log cfu。赤霞珠的g -1至4.83 log cfu。g -1在Feteasca regala。本研究共分离得到显微真菌627株。真菌数量最多的是交替菌属和青霉属(100%)。曲霉菌(76.92%)和枝孢菌(76.92%)也检出频率较高,但相对密度较小。从葡萄果实中分离到互交菌、黑曲霉、曲霉、灰葡萄孢、枝孢霉、扩张青霉、光斑霉、根霉和木霉等菌种。
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引用次数: 2
Antioxidant, compositional evaluation and blood pressure modulating potentials of Bryophyllum pinnatum (Lam.), Viscum album (L.) and Artocarpus altilis (Parkinson) leave extracts 青叶、荆芥和山茱萸叶提取物的抗氧化、成分评价和血压调节电位
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-13 DOI: 10.5219/912
Oluronke Ruth Osunlana, M. Bello, J. Johnson, O. B. Afolabi
Bryophyllum pinnatum (Lam.), Viscum album (L.) and Artocarpus altilis (Parkinson) are medicinal plants widely used based on their ethnomedicinal properties in the regulation of blood pressure. This study was designed to evaluate the antioxidant activities and compositional constituents of these plants. The antioxidant potentials were analyzed using DPPH and FRAP assays, while Folin-Ciocalteu method was employed in the determination of the total phenolic antioxidant contents. Compositional analyses of the leave extracts were determined using Gas Chromatograghy-Mass Spectrophotometer (GC-MS). The total phenolic contents in Bryophyllum pinnatum, Artocarpus altilis and Viscum album were revealed as; 659.50 ±0.02, 1667.50 ±0.03, 1232.00 ±0.02 mg GAE.100 g -1 respectively. Considering the antioxidant activities, Artocarpus altilis leaf extract showed inhibitory activity on DPPH with IC 50 of 2.24 ±0.26 mg.mL -1 , Bryophyllum pinnatum and Viscum album with IC 50 values 3.63 ±0.07 and 4.65 ±0.06  mg.mL -1 respectively.The FRAP in mg.GAE -1 for Artocarpus altilis , Bryophyllum pinnatum and Viscum album revealed; 2505.20 ±0.04, 1561.80 ±0.01 and 1698.00 ±0.03 respectively. GC-MS identified some vital phenolic components and essential fatty acids in the plants. The findings therefore suggest that; the plants if properly utilized, it could serve as alternatives in regulating blood pressure. Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4
苔藓植物(brophyllum pinnatum)、石竹(Viscum album)和金菖蒲(Artocarpus altilis)是因其具有调节血压的民族医药特性而被广泛应用的药用植物。本研究旨在评价这些植物的抗氧化活性及其组成成分。采用DPPH法和FRAP法测定其抗氧化电位,采用Folin-Ciocalteu法测定其总酚类抗氧化剂含量。采用气相色谱-质谱联用仪(GC-MS)对叶提取物进行成分分析。青叶、山茱萸和荆芥相册中总酚含量为;GAE.100 g -1分别为659.50±0.02、1667.50±0.03、1232.00±0.02 mg。从抗氧化活性来看,荆芥叶提取物对DPPH具有抑制作用,ic50为2.24±0.26 mg。mL -1中,青叶和粘子的ic50分别为3.63±0.07和4.65±0.06 mg。mL -1。FRAP在mg。揭示了石竹(Artocarpus altilis)、石竹(Bryophyllum pinnatum)和石竹(visum)的GAE -1;分别为2505.20±0.04、1561.80±0.01、1698.00±0.03。GC-MS鉴定了植物中一些重要的酚类成分和必需脂肪酸。因此,研究结果表明;如果利用得当,这种植物可以作为调节血压的替代品。正常0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4
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引用次数: 1
In vitro antioxidant potential and inhibitory effect of hydro-ethanolic extract from African black velvet tamarind (Dialium indium) pulp on type 2 diabetes linked enzymes 非洲黑绒罗罗子(Dialium indium)果肉水乙醇提取物对2型糖尿病相关酶的体外抗氧化潜力和抑制作用
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-13 DOI: 10.5219/911
O. B. Afolabi, O. Oloyede, A. Ojo, Amos Adeyinka Onansanya, S. Agunbiade, B. Ajiboye, J. Johnson, O. A. Peters
The alarming rate of diabetes mellitus (DM) globally is bothersome and has drawn the search light of researchers on naturally endowed phytonutrients being an alternative in managing the menace. Therefore, the current study was designed to investigate some antioxidant parameters embedded in the extract of Dialium indium ( DI ) fruit pulp and also, to elucidate its antidiabetic potentials through the inhibition of two key carbohydrate-metabolizing enzymes such as α-amylase and intestinal α-glucosidase. Hydro-ethanolic extract of DI fruit pulp was used for the antioxidants and enzyme inhibitory bioassays through various convectional antioxidant assay methods in vitro . In the results, total phenolic content of the extract had; 6.74 ±3.38 mg GAE.g -1 , total flavonoid contents; 0.02 ±0.01 mg QE.g -1 and FRAP; 0.84  ±0.47 mg AAE.g -1 dried sample. Also, there was a marked significant ( p <0.05) difference observed in the inhibition of α-amylase and intestinal α-glucosidase by the different concentrations of the extract used in concentration-dependent manner with their different EC 50 . The inhibition demonstrated against these two carbohydrate metabolizing enzymes possibly could be through the embedded antioxidant potentials of the fruit pulp and this if properly harnessed, it could be helpful in the management of type 2 diabetes.
全球范围内糖尿病(DM)的惊人发病率令人担忧,并引起了研究人员对天然植物营养素作为控制威胁的另一种选择的研究。因此,本研究旨在研究Dialium indium果肉提取物中的抗氧化参数,并通过抑制α-淀粉酶和肠道α-葡萄糖苷酶两种关键的糖代谢酶来阐明其降糖作用。采用各种常规体外抗氧化测定方法,对DI果肉水乙醇提取物进行抗氧化和酶抑制生物测定。结果表明,提取物总酚含量为;6.74±3.38 mg GAE。G -1,总黄酮含量;0.02±0.01 mg QE。g -1和FRAP;0.84±0.47 mg AAE。G -1干燥样品。不同浓度(浓度依赖性)提取物对肠道α-淀粉酶和α-葡萄糖苷酶的抑制作用差异显著(p <0.05)。对这两种碳水化合物代谢酶的抑制可能是通过果肉的抗氧化潜力来实现的,如果加以适当利用,它可能有助于2型糖尿病的治疗。
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引用次数: 3
Acceptability and sensory evaluation of energy bars and protein bars enriched with edible insect 添加食用昆虫的能量棒和蛋白质棒的可接受性和感官评价
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-13 DOI: 10.5219/925
Martina Adamek, A. Adámková, J. Mlček, M. Borkovcová, M. Bednářová
For consumers, one of the basic criteria of choosing a foodstuff, apart from nutritional values, is their taste and smell. In edible insect as a novel food, these criteria are not quite decisive. The main criterion in the Western countries is the acceptability of the food. This work deals with sensory evaluation of protein and energy bars, enriched with cricket flour from American and Czech producers, and their acceptability for consumers from the Czech Republic. The sensory evaluation was done using the questionnaire survey and a simple electronic nose. The survey has shown that edible insect bars are acceptable as a new type of food for consumers in the Czech Republic. Best rated by consumers were orange and pineapple flavour bars from the Czech manufacturer. Statistically significant difference was not detected between evaluation of the bars from the American and Czech manufacturers. Also, the difference between the bars of different flavours from the Czech producer was evaluated using a simple machine - a portable electronic nose. There was not a statistically significant difference between bars of different flavours from the American manufacturer. The positive contribution of the survey is that more than 80% of consumers are willing to consume food enriched with edible insect. This fact shows a change in public attitude to these foods.
对于消费者来说,除了营养价值外,选择食物的一个基本标准是它的味道和气味。在食用昆虫作为一种新型食物的情况下,这些标准并不十分具有决定性。西方国家的主要标准是食物的可接受性。这项工作涉及蛋白质和能量棒的感官评估,从美国和捷克生产商富含蟋蟀粉,以及他们对捷克共和国消费者的接受程度。感官评价采用问卷调查法和简易电子鼻法。调查显示,食用昆虫棒作为一种新型食品,在捷克共和国的消费者是可以接受的。消费者评价最高的是捷克制造商生产的橙味和菠萝味巧克力棒。在美国和捷克制造商对棒材的评价中没有发现统计学上的显著差异。此外,捷克生产商生产的不同口味的巧克力棒之间的差异是用一种简单的机器——便携式电子鼻来评估的。美国制造商生产的不同口味的巧克力棒之间没有统计学上的显著差异。调查的积极贡献是,超过80%的消费者愿意食用富含食用昆虫的食品。这一事实表明公众对这些食品的态度发生了变化。
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引用次数: 45
Wheat flour, bread and biscuits enriched by linseed fibre – comparison of harvest year, linseed variety and addition level 亚麻籽纤维富集小麦粉、面包和饼干——收获年份、亚麻籽品种和添加量的比较
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-11 DOI: 10.5219/870
M. Hrušková, I. Švec
Wheat flour was fortified by 2.5, 5.0 or 10 wt. per cent of linseed fibre, gained from seeds of golden flax varieties Amon and Raciol and brown one Recital (granulation 500 - 700 m), prepared from 2015 and 2016 harvests. Using analytical tests, namely sedimentation according to Zeleny and Falling Number, basic technological quality of flour composites was mostly independent on all three observed factors (harvest year, linseed variety and addition). Rheological tests included the farinograph, the extensigraph and the amylograph proofs. Enhancement by brown and yellow flax fibre significantly contributed to rise of farinograph water absorption and to dough stability shortening, directed mainly by addition level. Extensigraph curves course depended on dough resting time, higher differences between wheat control and flour composites were observed after 60 min dough resting. Linseed fibre weakened dough extensibility, and energy as area under curve also partially decreased about 3, 8 and 25% in average as portion of alternative materials in dough has risen. Compared to control, suspension viscosities of tested flour composite generally increased; the strongest effect was recorded for composite samples from harvest year 2016. During dough leavening, tested samples were differentiated according to maturograph dough resistance, and interaction of all three factors was identified. Regardless to variantion in dough machinability, specific volumes of composite bread samples were similar through whole sample set - any unequivocal trend was found. Somewhat worse vaulting of bread was calculated for buns manufactured from raw materials of 2016 harvest. Reversely, linseed fibre produced in 2015 improved crumb softness, especially at 5% enhancement (about ca 50% in average). The lowest addition of linseed fibre resctricted biscuits spread during baking in the highest extent, but rising level of enhancement suppressed elevated dough elasticity. Both cereal products were considered as acceptable for common consumers. Multivariate PCA method verified changes mainly in protein visco-elastic properties, which were reflected in bread and biscuits quality in an opposite manner. Based on this statistics, quality of wheat controls was comparable in both harvest year if related to changes induced by linssed fibre. In opposite to this, technological and consumer's parameters of flour composites and manufactured cereal products were statistically dependent of harvest year of linseed. As presumed, the lowest addition level brough the smallest changes; multiplied fortification caused gradual variation in results of all conducted proofs. Owing to high dietary fibre content in linseed fibre (over 50%), the medium dosage of the alternative material (i.e. 5% addition) could be recommended for praxis.
小麦粉中添加了2.5%、5.0或10%的亚麻籽纤维,这些亚麻籽来自金色亚麻品种Amon和Raciol以及棕色亚麻品种Recital(颗粒500 - 700米)的种子,这些亚麻籽是从2015年和2016年收获的。通过分析试验,即根据Zeleny和Falling Number进行沉淀,面粉复合材料的基本技术质量基本上与所有三个观察因素(收获年份、亚麻籽品种和添加量)无关。流变学试验包括淀粉样、拉伸样和淀粉样。棕色和黄色亚麻纤维对面粉吸水率的提高和面团稳定性的缩短有显著的促进作用,主要受添加量的影响。拉伸曲线的变化与面团静息时间有关,静息60 min后小麦对照与面粉复合材料的拉伸曲线差异较大。亚麻籽纤维降低了面团的延伸性,曲线下面积能量随替代材料用量的增加而平均下降了3、8、25%。与对照相比,被试复合面粉的悬浮粘度普遍升高;2016年收获年的复合样品效果最强。在面团发酵过程中,根据面团阻力对样品进行了区分,并确定了三者的相互作用。不管面团可加工性的变化,复合面包样品的具体体积是相似的,通过整个样品集-任何明确的趋势被发现。用2016年收获的原料制作的面包的拱度稍差。相反,2015年生产的亚麻籽纤维改善了面包屑的柔软度,特别是提高了5%(平均约为50%)。最低亚麻籽纤维添加量最大程度地限制了饼干在烘烤过程中的扩散,但增加亚麻籽纤维添加量抑制了面团弹性的提高。这两种谷物产品被认为是普通消费者可以接受的。多元主成分分析方法验证了蛋白质粘弹性的变化,而蛋白质粘弹性的变化在面包和饼干质量上的反映则相反。根据这一统计数据,如果与剥落纤维引起的变化有关,两个收获年度的小麦质量对照是相当的。与此相反,面粉合成物和谷物制品的工艺参数和消费者参数与亚麻籽收获年份有统计学相关性。可以推测,最低添加水平带来的变化最小;多次设防导致所有进行的证明的结果逐渐变化。由于亚麻籽纤维的膳食纤维含量较高(超过50%),可推荐中等剂量的替代材料(即添加5%)用于实践。
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引用次数: 1
The morphological and antioxidant characteristics of inflorescences within wild-growing genotypes of elderberry (Sambucus nigra L.) 野生接骨木(Sambucus nigra L.)基因型花序形态及抗氧化特性研究
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-05 DOI: 10.5219/919
V. Sedláčková, O. Grygorieva, K. Fatrcová-Šramková, O. Vergun, Y. Vinogradova, E. Ivanišová, J. Brindza
The aim of this study was to determine the basic morphological characteristics (weight, length) and antioxidant activity (using DPPH method) of elderberry ( Sambucus nigra L.) inflorescences as well as some elderberry-derived food products prepared from fresh (honey, alcoholic extract, tea infusions) and dried inflorescences (syrup). For the study of problematic, it was used 113 wild-growing genotypes of elderberry from 56 locations in Slovakia growing at an altitude of 98.15 - 712.32 m. The weight of fresh inflorescences ranged from 0.45 to 57.59 g (75.65% coefficient of variation value), the total length of inflorescences ranged from 19.0 to 282.0 mm (22.42%), the length of inflorescence´s stems from 9.0 to 197.0 mm (31.51%), a number of petals predominated pentanumerous petals. Variability in primary and secondary branching reported a low to high degree of variability among as well as within the genotypes. Results showed significant differences in the shape of inflorescences and the colour of flowers among each genotype. Antioxidant activity by DPPH method in elderberry inflorescence water extract was between 85.12 and 89.29%. Activation of tea infusions and beverages was ensured using a mechanism Kalyxx based on galvanic effect. In beverages made from 10% diluted honey prepared from fresh inflorescences in the carbohydrate-based extract, anti-radical activity was determined in the range of 16.81 - 24.16%. In an alcoholic extract from fresh inflorescences, anti-radical activity was between 90.99 and 93.16%. In beverages acquired from the syrup of flowers, we identified antioxidant activity ranging from 37.92 (10%) to 62.82% (40%). Results indicated that elderberry inflorescences and elderberry-derived food products can be attractive to consumers and in future can increase the assortments of healthy products.
本研究的目的是测定接骨木(Sambucus nigra L.)花序的基本形态特征(重量、长度)和抗氧化活性(DPPH法),以及一些由新鲜(蜂蜜、酒精提取物、茶浸膏)和干燥(糖浆)的接骨木衍生食品。为了研究问题,它使用了来自斯洛伐克56个地点生长在海拔98.15 - 712.32 m的113个野生基因型接骨木。鲜花序重量为0.45 ~ 57.59 g(变异系数值为75.65%),花序总长度为19.0 ~ 282.0 mm(22.42%),花序茎长为9.0 ~ 197.0 mm(31.51%),花瓣数以五朵花瓣为主。据报道,初级和次级分支的变异性在基因型之间和基因型内部具有低到高的变异性。结果表明,各基因型在花序形状和花的颜色上存在显著差异。DPPH法测定接骨木花序水提物的抗氧化活性为85.12 ~ 89.29%。利用基于电效应的机制Kalyxx确保茶泡和饮料的活化。在以碳水化合物为基础提取的新鲜花序制成的10%稀释蜂蜜饮料中,抗自由基活性在16.81 - 24.16%之间。鲜花序醇提物抗自由基活性在90.99 ~ 93.16%之间。在从花糖浆中提取的饮料中,我们发现抗氧化活性从37.92(10%)到62.82%(40%)不等。结果表明,接骨木果花序和接骨木果衍生食品对消费者具有吸引力,未来可以增加健康产品的种类。
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引用次数: 20
Vicia villosa protein isolate: a new source of protein to make a biodegradable film 绒毛维纶蛋白分离物:一种新的蛋白质来源,可制成可生物降解的薄膜
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-05 DOI: 10.5219/913
F. Attar, F. Rezagholi, M. A. Hesarinejad
Edible films from Vicia villosa protein isolate (VVPI) containing different contents of glycerol and sorbitol (30, 40, 50 and 60%w/w of protein) were developed. The aim of this study was to investigate the influence of type and concentration of plasticizers on the properties of edible films obtained from VVPI. Type and concentration of plasticizer significantly (p <0.05) affected the mechanical, barrier, thermal and surface properties as well as opacity of the films. As plasticizer concentration increased, tensile strength decreased concomitant with increase in elongation at break and water vapor permeability. The similar trend behavior was observed for the film solubility, which increased with increasing plasticizer concentration. Sorbitol plasticized films, showed higher film solubility compared to glycerol plasticized films. Sorbitol plasticized films provided the most tensile strength values; however, its effect on water vapor permeability was low. In contrast, glycerol plasticized films exhibited the least tensile strength values, resulting in increasing water vapor permeability. Opacity of glycerol plasticized films was lower than that of sorbitol plasticized films, and decreased with increasing plasticizer content ( p <0.05). Also, a significant decrease ( p <0.05) was observed in thermal features and surface hydrophobicity values with increasing in plasticizer contents. It was observed that the films plasticized with sorbitol had lower moisture content than those with glycerol.
用不同含量的甘油和山梨醇(30、40、50和60%w/w)制备了绒毛豌豆蛋白分离物(VVPI)的可食用膜。研究了增塑剂种类和浓度对VVPI可食性薄膜性能的影响。增塑剂的种类和浓度对膜的力学性能、阻隔性、热性能、表面性能和不透明度有显著影响(p <0.05)。随着增塑剂浓度的增加,拉伸强度降低,断裂伸长率和透气性增加。膜溶解度随增塑剂浓度的增加而增加,具有相似的趋势。山梨醇塑化膜的溶解度比甘油塑化膜高。山梨醇塑化膜的抗拉强度值最高;但其对水蒸气渗透性的影响较小。相比之下,甘油塑化膜的拉伸强度值最小,导致水蒸气透气性增加。甘油塑化膜的不透明度低于山梨醇塑化膜,且随着增塑剂含量的增加而降低(p <0.05)。随着增塑剂含量的增加,热特性和表面疏水性显著降低(p <0.05)。结果表明,山梨醇塑化膜的含水率比甘油塑化膜低。
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引用次数: 4
Fungal diversity in the grapes-to-wines chain with emphasis on Penicillium species 葡萄到葡萄酒链中的真菌多样性,重点是青霉菌物种
Q3 Agricultural and Biological Sciences Pub Date : 2018-04-04 DOI: 10.5219/882
Soňa Felšöciová, Z. Mašková, M. Kačániová
The aim of this work was the description of surface and endogenous mycobiota colonisation of grapes, fresh grape juice, grape must, and wine primarily focused to the current spectrum of the penicillium species. One sample of white grape variety Palava and one sample of blue grape variety Dornfelder were collected in Small Carpathian wine growing region of Slovakia in the year 2017. Direct plating of grapes on agar plates was used for analysis of surface mycobiota of grapes while surface sterilsed grapes were used for endogenous mycobiota analysis. Mycobiota of juice, must, and wine was analysed by plate dilution method. Overall, we isolated 148 strains belonging to 13 genera of filamentous microscopic fungi and Mycelia sterilia from grape variety Palava, while the most frequent was Alternaria . Alternaria was the most common genus in the surface and endogenous colonisation with an average relative density 50% and 73.6%, respectively. A total of 2 species of Penicillium were detected from the grapes to wine, potentially toxigenic Penicillium expansum and P. chrysogenum . A total of 39 strains belonging to 6 genera and Mycelia sterilia were identified from grape variety Dornfelder. The most abundant genus was also Alternaria (51.3%), followed by Penicillium (12.8%). Alternaria was the most common genus in the surface and endogenous colonisation  and fresh grape juice with an average relative density from 20% (grape juice) to 71% (endogenous colonisation of grapes). A total of 3 species of Penicillium were detected from the grapes to wine, where Penicillium expansum were detected most commonly. In the second part of our work some selected isolates were tested to the ability to produce mycotoxins such as patulin, citrinin, and roquefortin C in in vitro condition by thin layer chromatography method. All tested  strains of Penicillium species were able to produce at least one mycotoxin.  Normal 0 21 false false false EN-GB X-NONE X-NONE
这项工作的目的是描述葡萄、新鲜葡萄汁、葡萄汁和葡萄酒的表面和内源性真菌定殖,主要集中在青霉菌物种的当前光谱上。2017年,我们在斯洛伐克的小喀尔巴阡葡萄酒产区采集了一份白葡萄品种Palava和一份蓝葡萄品种Dornfelder。葡萄直接镀在琼脂板上进行葡萄表面菌群分析,表面灭菌的葡萄进行内生菌群分析。采用平板稀释法对果汁、葡萄酒和葡萄酒中的真菌菌群进行了分析。从Palava葡萄品种中分离到丝状显微真菌和无菌菌丝共13属148株,其中以Alternaria最为常见。地表定殖和内源定殖最常见的属为互花属,平均相对密度分别为50%和73.6%。从葡萄到葡萄酒共检出2种青霉菌,分别为具有潜在毒力的扩张青霉菌和黄霉青霉菌。从葡萄品种多恩菲尔德(Dornfelder)中共鉴定出6属39株菌丝体和绝育菌丝。真菌种类最多的属为交替菌属(51.3%),其次为青霉属(12.8%)。在地表和内源定殖和新鲜葡萄汁中最常见的属是Alternaria,平均相对密度为20%(葡萄汁)~ 71%(葡萄内源定殖)。从葡萄到葡萄酒共检出青霉3种,其中以扩张青霉最多检出。在第二部分的工作中,我们选择了一些分离菌株,用薄层色谱法测试了它们在体外条件下产生霉菌毒素的能力,如曲霉素、柑桔霉素和骆家福素C。所有被测试的青霉菌菌株都能产生至少一种霉菌毒素。正常0 21 false false false EN-GB X-NONE X-NONE
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引用次数: 6
Comparing the quality of honey from beekeepers and honey from the market chain 比较养蜂人生产的蜂蜜和市场上销售的蜂蜜的质量
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-29 DOI: 10.5219/909
M. Bušová, L. Kouřimská
Honey is a valuable food for its beneficial nutritional and dietetic effects. The quality of honey fluctuates considerably according to various criteria, the adulteration of honey with cheaper substitutes is not negligible. The quality of honey in the market chain with honey taken from beekeepers was compared in this study. A total of 10 samples from each group were tested for basic qualitative markers and compared with legislative criteria. The samples were analysed for fructose and glucose content, water content, titratable acidity and two tests for illegal sugar additions. The results revealed the addition of 25% of the technical syrup in one sample of honey from the market chain, one sample had the sum of fructose and glucose 56,3%, it is below the required limit 60% (differed by 6,3%). In other parameters the samples complied with the valid legislation. All tested parameters in honey from beekeepers met the criteria of the legislation, only 1 sample of blossom honey had the sum of fructose and glucose just below the required limit. The sum of fructose and glucose in this sample was 58.3 %, it differed by 2.9% from the required content of 60%. Sensory analysis was used to assess four samples of honey from beekeepers collected by different techniques. Results have not shown significant difference in sensory properties between manually pressed honey and honey obtained after whirling. The responses characterizing the favourable sensory properties of the examined honey samples were prevailing. The difference between the perception of honey after whirling and honey harvested by press manually was not demonstrated in sensory properties.
蜂蜜因其有益的营养和饮食作用而成为一种宝贵的食物。根据各种标准,蜂蜜的质量波动很大,蜂蜜中掺入更便宜的替代品是不可忽视的。本研究比较了养蜂人蜂蜜在市场链上的质量。每组各取10个样本进行基本定性标记检测,并与立法标准进行比较。对这些样品进行了果糖和葡萄糖含量、含水量、可滴定酸度和两项非法添加糖的测试。结果显示,在市场链的一份蜂蜜样品中添加了25%的技术糖浆,一份样品中果糖和葡萄糖的总和为56.3%,低于规定限值60%(相差6.3%)。在其他参数中,样品符合有效法规。养蜂人生产的蜂蜜的所有测试参数均符合立法标准,只有1个花蜜样本的果糖和葡萄糖的总和略低于规定的限度。该样品中果糖和葡萄糖的含量之和为58.3%,与60%的要求含量相差2.9%。采用感官分析方法对四种不同技术采集的养蜂人蜂蜜样本进行了评价。结果表明,手工压榨的蜂蜜和旋转后得到的蜂蜜的感官特性没有显著差异。反应表征良好的感官特性的检查蜂蜜样品是普遍存在的。旋转后的蜂蜜和手动压榨收获的蜂蜜的感知差异在感官特性上没有得到证明。
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引用次数: 5
Mineral composition of amaranth (Amaranthus L.) seeds of vegetable and grain usage by ARHIVBSP selection 用ARHIVBSP筛选蔬菜和谷物中苋菜种子的矿物成分
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-29 DOI: 10.5219/863
M. Gins, V. Gins, S. Motyleva, I. Kulikov, S. Medvedev, P. F. Kononkov, V. Pivovarov
The questions of the practical usage of the analytical scanning electron microscope JSM 600 LA by JEOL company (Japan) with EDS system - microanalysis for the studying of the ash elemental composition of seeds 9 breeds (Vegetable and Grain application) 4 species genus Amaranthus L. - A. hypochondriacus, A. cruentus, A. hybridus, A. caudatus, A. tricolor. Plant seeds by Federal center of vegetable production selection were envisaged. We studied the concentration of 14 basic elements (in weight %) contained in the mineral part of amaranth seeds. In the amaranth seeds of vegetable forms the accumulation order of the elements is the following: Ca >K >P >Mg >Si >Se >Fe >Mo ≈ S ≈ Cl ≈ Zn >Na >Al. In the seeds of the grain forms the order is different: К >P >Ca >Si >Se >Mg >Fe >Na >Mo >Cl ≈ S ≈ Mn ≈ Zn ≈ Al. The amaranth seeds of the grain forms are rich in macro - and microelements. P, K, Cl and S in the seeds of the grain forms are accumulated on 50, 37, 15 and 5% more and Si, Fe and Al in 2.6 and 1.8 times more than in the vegetable forms seeds. The breeds with the high concentration of the elements are recommended for using in the selection process. The elevated level of the essential macro- and microelements such as Ca, K, P, Mg, Mo, S and Cl stipulates the perspective of the functional products creation on the base of the studied amaranth seeds for the enrichment of the food stuffs.
日本JEOL公司JSM 600 LA分析扫描电子显微镜与EDS系统的应用问题-微量分析对苋属9个品种(蔬菜和粮食应用)4种种子的灰分元素组成进行了研究- A. hypochondriacus, A. cruentus, A. hybridus, A. caudatus, A. tricolor。设想由联邦蔬菜生产中心选择植物种子。研究了苋菜种子矿物部分中14种基本元素的含量(以重量%计)。在苋菜种子的蔬菜形态中,元素的积累顺序为:Ca >K >P >Mg >Si >Se >Fe >Mo≈S≈Cl≈Zn >Na >Al。籽粒形态的种子中元素含量顺序为К >P >Ca >Si >Se >Mg >Fe >Na >Mo >Cl≈S≈Mn≈Zn≈Al。籽粒形态的苋菜种子中常量元素和微量元素含量均较丰富。籽型种子中磷、钾、氯、硫的积累量分别比蔬菜型种子高50、37、15、5%,Si、Fe、Al的积累量分别是蔬菜型种子的2.6、1.8倍。建议在选择过程中使用这些元素浓度高的品种。苋菜种子中钙、钾、磷、镁、钼、硫、氯等必需宏、微量元素含量的提高,为以所研究的苋菜种子为基础,进行功能性产品的开发提供了前景。
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引用次数: 13
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Potravinarstvo
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