Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0510B
N. T. Tran, Taichi Oguchi, E. Matsunaga, A. Kawaoka, Kazuo N. Watanabe, A. Kikuchi
Novel transgenic Eucalyptus camaldulensis trees expressing the bacterial choline oxidase A (codA) gene by the Cauliflower mosaic virus (CaMV) 35S promoter and the Arabidopsis thaliana heat shock protein (HSP) terminator was developed. To evaluate the codA transcription level and the metabolic products and abiotic stress tolerance of the transgenic trees, a six-month semi-confined screen house cultivation trial was conducted under a moderate-stringency salt-stress condition. The transcription level of the CaMV 35S promoter driven-codA was more than fourfold higher, and the content of glycine betaine, the metabolic product of codA, was twofold higher, with the HSP terminator than with the nopaline synthase (NOS) terminator. Moreover, the screen house cultivation revealed that the growth of transgenic trees under the salt stress condition was alleviated in correlation with the glycine betaine concentration. These results suggest that the enhancement of codA transcription by the HSP terminator increased the abiotic stress tolerance of Eucalyptus plantation trees.
{"title":"Transcriptional enhancement of a bacterial choline oxidase A gene by an HSP terminator improves the glycine betaine production and salinity stress tolerance of Eucalyptus camaldulensis trees.","authors":"N. T. Tran, Taichi Oguchi, E. Matsunaga, A. Kawaoka, Kazuo N. Watanabe, A. Kikuchi","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0510B","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0510B","url":null,"abstract":"Novel transgenic Eucalyptus camaldulensis trees expressing the bacterial choline oxidase A (codA) gene by the Cauliflower mosaic virus (CaMV) 35S promoter and the Arabidopsis thaliana heat shock protein (HSP) terminator was developed. To evaluate the codA transcription level and the metabolic products and abiotic stress tolerance of the transgenic trees, a six-month semi-confined screen house cultivation trial was conducted under a moderate-stringency salt-stress condition. The transcription level of the CaMV 35S promoter driven-codA was more than fourfold higher, and the content of glycine betaine, the metabolic product of codA, was twofold higher, with the HSP terminator than with the nopaline synthase (NOS) terminator. Moreover, the screen house cultivation revealed that the growth of transgenic trees under the salt stress condition was alleviated in correlation with the glycine betaine concentration. These results suggest that the enhancement of codA transcription by the HSP terminator increased the abiotic stress tolerance of Eucalyptus plantation trees.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"215-224"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0510B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48099410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0502A
M. Bagues, Behrooz Sarabi, J. Ghashghaie, I. Souli, K. Nagaz
Arid and semiarid regions with rain shortage and scarce good quality water must make use of low-quality water for irrigation. Consequently, improved plant cultivars for use in these areas should show adaptation capacities to confer drought and salt resistance and allow the cultivation under limited water availabiltiy. The present study was conducted to determine the effect of deficit irrigation with saline water on two local barley landraces, "Karkeni" and "Bengardeni". Plants were saline-irrigated with three watering regimes during tillering, heading, and grain filling stages. Biochemical traits, carbon isotope discrimination (Δ13C), mineral composition, grain yield (GY) and water use efficiency based on grain yield (WUEgy) were evaluated as performance indicators. Almost all of the studied traits (e.g. soluble carbohydrates, proline, ∆13C, Na concentration, and GY) were significantly affected by deficient saline-irrigation regimes at different growth stages. The hierarchical clustering analysis clearly showed that Δ13C placed very close to GY averaging two barley landraces, which was in accordance with the scatter plot result. Multiple linear regression performed between GY as the dependent variable and other traits studied as the independent variables indicated that WUEgy, Δ13C, and soluble carbohydrates significantly explained the variability in GY (R 2=95.64%). A significant positive correlation that observed between ∆13C and GY at three growth stages, indicated that ∆13C may be an important proxy component for indirect selection of yield potential in barley under deficient irrigation regimes with saline water. According to our result, "Karkeni" seems to be more efficient in terms of higher GY, WUEgy, proline and carbohydrate contents, K, Mg and Zn concentrations, as well as lower Δ13C and lipid peroxidation as compared with "Bengardeni", under low osmotic potential imposed by deficient irrigation treatments with saline water, "Karkeni" can thus be selected and used as a parent in order to obtain more tolerant plants against such stresses in future breeding programs.
{"title":"The validity of carbon isotope discrimination as a screening criterion for grain yield in two barley landraces under deficit irrigation with saline water in southern Tunisia.","authors":"M. Bagues, Behrooz Sarabi, J. Ghashghaie, I. Souli, K. Nagaz","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0502A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0502A","url":null,"abstract":"Arid and semiarid regions with rain shortage and scarce good quality water must make use of low-quality water for irrigation. Consequently, improved plant cultivars for use in these areas should show adaptation capacities to confer drought and salt resistance and allow the cultivation under limited water availabiltiy. The present study was conducted to determine the effect of deficit irrigation with saline water on two local barley landraces, \"Karkeni\" and \"Bengardeni\". Plants were saline-irrigated with three watering regimes during tillering, heading, and grain filling stages. Biochemical traits, carbon isotope discrimination (Δ13C), mineral composition, grain yield (GY) and water use efficiency based on grain yield (WUEgy) were evaluated as performance indicators. Almost all of the studied traits (e.g. soluble carbohydrates, proline, ∆13C, Na concentration, and GY) were significantly affected by deficient saline-irrigation regimes at different growth stages. The hierarchical clustering analysis clearly showed that Δ13C placed very close to GY averaging two barley landraces, which was in accordance with the scatter plot result. Multiple linear regression performed between GY as the dependent variable and other traits studied as the independent variables indicated that WUEgy, Δ13C, and soluble carbohydrates significantly explained the variability in GY (R 2=95.64%). A significant positive correlation that observed between ∆13C and GY at three growth stages, indicated that ∆13C may be an important proxy component for indirect selection of yield potential in barley under deficient irrigation regimes with saline water. According to our result, \"Karkeni\" seems to be more efficient in terms of higher GY, WUEgy, proline and carbohydrate contents, K, Mg and Zn concentrations, as well as lower Δ13C and lipid peroxidation as compared with \"Bengardeni\", under low osmotic potential imposed by deficient irrigation treatments with saline water, \"Karkeni\" can thus be selected and used as a parent in order to obtain more tolerant plants against such stresses in future breeding programs.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"193-206"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0502A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46515016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0528A
Natsuko Kinoshita, S. Betsuyaku
This paper is about the cellular responses of plants to chewing insect attacks. We deployed a recently developed experimental system to monitor the responsiveness of Arabidopsis thaliana (Arabidopsis) to the application of oral secretion (OS) from Lepidopteran generalist herbivore Spodoptera litura (S. litura). Oral secretion from S. litura contains gut regurgitant and saliva. We identified significant differences in the wound closure morphologies (e.g., dried and sealed tissue) between mechanically damaged leaves with and without an application of S. litura OS at the site-of-injury. Experimental controls were mechanically wounded leaves. Wounds were walled off by visible vertical cross sections. Cell death was restricted to the immediate areas of the wounds. In contrast, mechanically damaged leaves treated with S. litura OS did not display a clear sealing pattern due to an absence of a defined vertical cross section at the wound site. Notably, OS treated leaves exhibited a wider area of visible premature senescence (the declining of chlorophyll content caused by death of chloroplasts) around the injury than controls. More pronounced senescence was also observed around the injury in S. litura OS treated wounds than in controls. Heat inactivated S. litura OS elicited a similar response to non-heat inactivated samples. The causal compound is heat stable and thus not a protein. Our results suggest that S. litura OS: (1) inhibited wound recovery responses in leaves; (2) promoted senescence around injured areas. The function of senescence may be to relocate nutritional resources to support plant survival when attacked.
{"title":"The effects of Lepidopteran oral secretion on plant wounds: A case study on the interaction between Spodoptera litura and Arabidopsis thaliana.","authors":"Natsuko Kinoshita, S. Betsuyaku","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0528A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0528A","url":null,"abstract":"This paper is about the cellular responses of plants to chewing insect attacks. We deployed a recently developed experimental system to monitor the responsiveness of Arabidopsis thaliana (Arabidopsis) to the application of oral secretion (OS) from Lepidopteran generalist herbivore Spodoptera litura (S. litura). Oral secretion from S. litura contains gut regurgitant and saliva. We identified significant differences in the wound closure morphologies (e.g., dried and sealed tissue) between mechanically damaged leaves with and without an application of S. litura OS at the site-of-injury. Experimental controls were mechanically wounded leaves. Wounds were walled off by visible vertical cross sections. Cell death was restricted to the immediate areas of the wounds. In contrast, mechanically damaged leaves treated with S. litura OS did not display a clear sealing pattern due to an absence of a defined vertical cross section at the wound site. Notably, OS treated leaves exhibited a wider area of visible premature senescence (the declining of chlorophyll content caused by death of chloroplasts) around the injury than controls. More pronounced senescence was also observed around the injury in S. litura OS treated wounds than in controls. Heat inactivated S. litura OS elicited a similar response to non-heat inactivated samples. The causal compound is heat stable and thus not a protein. Our results suggest that S. litura OS: (1) inhibited wound recovery responses in leaves; (2) promoted senescence around injured areas. The function of senescence may be to relocate nutritional resources to support plant survival when attacked.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"237-242"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0528A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46322966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0508A
S. Jang, Hsing-Yi Li
Recently, two rice genes, OsAPETALA2 (OsAP2) and OsWRKY24 have been reported to be positive regulators involved in increased lamina inclination and grain size through cell elongation. Here, we found that the two genes have tightly linked expression patterns and functional convergence in rice, and are also likely to play an opposite role in Arabidopsis. Overexpression of the two rice transcription factors in Arabidopsis caused smaller plant size with reduced cell size, and the expression of a series of genes encoding expansins and xyloglucan endotransglucosylase/hydrolases (XTHs) involved in cell elongation was reduced. However, transgenic Arabidopsis expressing OsWRKY24-SRDX as a synthetic chimeric repressor displayed indistinguishable phenotypes from wild-type plants. Moreover, the subcellular localization pattern of OsWRKY24 in Arabidopsis was different from that in rice. Thus, we demonstrate an example of transcription factors from one species playing distinct roles in different plant species.
{"title":"Overexpression of OsAP2 and OsWRKY24 in Arabidopsis results in reduction of plant size.","authors":"S. Jang, Hsing-Yi Li","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0508A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0508A","url":null,"abstract":"Recently, two rice genes, OsAPETALA2 (OsAP2) and OsWRKY24 have been reported to be positive regulators involved in increased lamina inclination and grain size through cell elongation. Here, we found that the two genes have tightly linked expression patterns and functional convergence in rice, and are also likely to play an opposite role in Arabidopsis. Overexpression of the two rice transcription factors in Arabidopsis caused smaller plant size with reduced cell size, and the expression of a series of genes encoding expansins and xyloglucan endotransglucosylase/hydrolases (XTHs) involved in cell elongation was reduced. However, transgenic Arabidopsis expressing OsWRKY24-SRDX as a synthetic chimeric repressor displayed indistinguishable phenotypes from wild-type plants. Moreover, the subcellular localization pattern of OsWRKY24 in Arabidopsis was different from that in rice. Thus, we demonstrate an example of transcription factors from one species playing distinct roles in different plant species.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"273-279"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0508A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42598869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0614A
Masashi Naruse, H. Takahashi, N. Kurata, Yukihiro Ito
The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.
{"title":"Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus.","authors":"Masashi Naruse, H. Takahashi, N. Kurata, Yukihiro Ito","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0614A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0614A","url":null,"abstract":"The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"267-272"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0614A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42448367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0507A
I. Nagahage, Shingo Sakamoto, M. Nagano, T. Ishikawa, M. Kawai‐Yamada, Nobutaka Mitsuda, M. Yamaguchi
The ARABIDOPSIS THALIANA ACTIVATION FACTOR 2 (ATAF2) protein has been demonstrated to be involved in various biological processes including biotic stress responses, photo morphogenesis, and auxin catabolism. However, the transcriptional function of ATAF2 currently remains elusive. Therefore, to further understand the molecular function of ATAF2, we evaluated the transcriptional activities of ATAF2 using a transient assay system in this study. We used an effector consisting of a GAL4-DNA binding domain (GAL4-BD) fused to ATAF2, and observed upregulated reporter gene expression, suggesting that ATAF2 potentially has transcriptional activation activity. ATAF2 has been shown to activate reporter gene expression under the control of the ORE1 promoter. By contrast, ATAF2 significantly repressed reporter gene expression driven by the NIT2 promoter. These data suggest that ATAF2 is a bifunctional transcription factor that can alter target gene expression depending on the promoter sequences.
{"title":"An NAC domain transcription factor ATAF2 acts as transcriptional activator or repressor dependent on promoter context.","authors":"I. Nagahage, Shingo Sakamoto, M. Nagano, T. Ishikawa, M. Kawai‐Yamada, Nobutaka Mitsuda, M. Yamaguchi","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0507A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0507A","url":null,"abstract":"The ARABIDOPSIS THALIANA ACTIVATION FACTOR 2 (ATAF2) protein has been demonstrated to be involved in various biological processes including biotic stress responses, photo morphogenesis, and auxin catabolism. However, the transcriptional function of ATAF2 currently remains elusive. Therefore, to further understand the molecular function of ATAF2, we evaluated the transcriptional activities of ATAF2 using a transient assay system in this study. We used an effector consisting of a GAL4-DNA binding domain (GAL4-BD) fused to ATAF2, and observed upregulated reporter gene expression, suggesting that ATAF2 potentially has transcriptional activation activity. ATAF2 has been shown to activate reporter gene expression under the control of the ORE1 promoter. By contrast, ATAF2 significantly repressed reporter gene expression driven by the NIT2 promoter. These data suggest that ATAF2 is a bifunctional transcription factor that can alter target gene expression depending on the promoter sequences.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"285-289"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0507A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44978167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0601A
H. Uchida, E. Mizohata, S. Okada
The green microalga Botryococcus braunii Showa, which produces large amounts of triterpene hydrocarbons, exclusively uses the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosyntheses, and the terminal enzyme in this pathway, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), is regarded as a light-dependent key regulatory enzyme. In order to investigate the possible association of HDR and ferredoxin in this organism, we constructed tertiary structure models of B. braunii HDR (BbHDR) and one of ferredoxin families in the alga, a photosynthetic electron transport F (BbPETF)-like protein, by using counterparts from E. coli and Chlamydomonas reinhardtii as templates, respectively, and performed docking analysis of these two proteins. After docked models are superimposed onto their counterpart proteins in a non-photosynthetic organism, Plasmodium falciparum, the BbPETF-like protein comes in contact with the backside of BbHDR, which was defined in a previous report (Rekittke et al. 2013), and the distance of the two Fe-S centers is 14.7 Å. This distance is in almost the same level as that for P. falicarum, 12.6 Å. To our knowledge, this is the first model suggesting the possible association of HDR with a ferredoxin in O2-evolving photosynthetic organisms.
{"title":"Docking analysis of models for 4-hydroxy-3-methylbut-2-enyl diphosphate reductase and a ferredoxin from Botryococcus braunii, race B.","authors":"H. Uchida, E. Mizohata, S. Okada","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0601A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0601A","url":null,"abstract":"The green microalga Botryococcus braunii Showa, which produces large amounts of triterpene hydrocarbons, exclusively uses the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosyntheses, and the terminal enzyme in this pathway, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), is regarded as a light-dependent key regulatory enzyme. In order to investigate the possible association of HDR and ferredoxin in this organism, we constructed tertiary structure models of B. braunii HDR (BbHDR) and one of ferredoxin families in the alga, a photosynthetic electron transport F (BbPETF)-like protein, by using counterparts from E. coli and Chlamydomonas reinhardtii as templates, respectively, and performed docking analysis of these two proteins. After docked models are superimposed onto their counterpart proteins in a non-photosynthetic organism, Plasmodium falciparum, the BbPETF-like protein comes in contact with the backside of BbHDR, which was defined in a previous report (Rekittke et al. 2013), and the distance of the two Fe-S centers is 14.7 Å. This distance is in almost the same level as that for P. falicarum, 12.6 Å. To our knowledge, this is the first model suggesting the possible association of HDR with a ferredoxin in O2-evolving photosynthetic organisms.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"297-301"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0601A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46479351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0612A
Nobuhiro Sasaki, A. Watanabe, T. Asakawa, Makoto Sasaki, Nobue Hoshi, Zenbi Naito, Y. Furusawa, T. Shimokawa, M. Nishihara
The development of new varieties of perennial plants generally requires lengthy and laborious procedures. In this study, we used ion beam irradiation mutagenesis in an attempt to accelerate the breeding process for perennial plants. We evaluated the biological effects of five ion beam sources (carbon, neon, argon, silicon, and iron) and neutron irradiation on Japanese gentian and apple. These treatments were applied at the National Institute of Radiological Sciences (NIRS) using the Heavy Ion Medical Accelerator in Chiba (HIMAC) and the Neutron-exposure Accelerator System for Biological Effect Experiments (NASBEE). Biological effects were observed in in vitro gentian plants after irradiation with ion beams at <10 Gy, whereas apple trees were less sensitive to ion beam irradiation. The growth of gentians in vitro was repressed by 3 Gy neutron irradiation, while that of grafted apple trees was not affected by 4 Gy neutron irradiation. During in vitro proliferation, seven pink-flowered lines were obtained from originally blue-flowered gentian after C and Ne ion beam irradiation treatments. Genomic and reverse transcription-PCR analyses of these lines suggested that the mutations occurred in the genomic region containing F3'5'H (encoding flavonoid 3',5'-hydroxylase). These results provide useful information for the mutagenesis and breeding of gentian, apple, and other perennial plants.
{"title":"Biological effects of ion beam irradiation on perennial gentian and apple.","authors":"Nobuhiro Sasaki, A. Watanabe, T. Asakawa, Makoto Sasaki, Nobue Hoshi, Zenbi Naito, Y. Furusawa, T. Shimokawa, M. Nishihara","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0612A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0612A","url":null,"abstract":"The development of new varieties of perennial plants generally requires lengthy and laborious procedures. In this study, we used ion beam irradiation mutagenesis in an attempt to accelerate the breeding process for perennial plants. We evaluated the biological effects of five ion beam sources (carbon, neon, argon, silicon, and iron) and neutron irradiation on Japanese gentian and apple. These treatments were applied at the National Institute of Radiological Sciences (NIRS) using the Heavy Ion Medical Accelerator in Chiba (HIMAC) and the Neutron-exposure Accelerator System for Biological Effect Experiments (NASBEE). Biological effects were observed in in vitro gentian plants after irradiation with ion beams at <10 Gy, whereas apple trees were less sensitive to ion beam irradiation. The growth of gentians in vitro was repressed by 3 Gy neutron irradiation, while that of grafted apple trees was not affected by 4 Gy neutron irradiation. During in vitro proliferation, seven pink-flowered lines were obtained from originally blue-flowered gentian after C and Ne ion beam irradiation treatments. Genomic and reverse transcription-PCR analyses of these lines suggested that the mutations occurred in the genomic region containing F3'5'H (encoding flavonoid 3',5'-hydroxylase). These results provide useful information for the mutagenesis and breeding of gentian, apple, and other perennial plants.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"249-257"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0612A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42836560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0529A
Mirai Azuma, Y. Oshima, Shingo Sakamoto, Nobutaka Mitsuda, M. Ohme-Takagi, S. Otagaki, S. Matsumoto, K. Shiratake
We had previously reported that the InMYB1 promoter, the 1023 bp upstream region of InMYB1, works petal-specifically in various dicot plants by recognizing petal identity at a cellular level. To determine the petal-specific region in the InMYB1 promoter, Arabidopsis plants harboring InMYB1_1023b::GUS (β-glucuronidase), InMYB1_713b::GUS, InMYB1_506b::GUS, InMYB1_403b::GUS, InMYB1_332b::GUS, InMYB1_200b::GUS and InMYB1_140b::GUS were produced and confirmed a shortest region, which has the petal-specific promoter activity by using histochemical GUS assay. Petal-specific GUS staining was not observed in the Arabidopsis plants transformed with InMYB1_200b::GUS and InMYB1_140b::GUS, but observed in transgenic Arabidopsis plants harboring from InMYB1_1023b::GUS to InMYB1_332b::GUS. cDNA sequence of InMYB1 shows that 120 bp upstream region of InMYB1 is 5' untranslated region, suggesting that the 332-121 bp upstream region of InMYB1 contains an important element for petal-specific gene expression. In the Arabidopsis harboring the InMYB1_332-121b×3_TATA_Ω::GUS, petal-specific GUS staining was observed and the staining was stronger than in the Arabidopsis harboring InMYB1_1023b::GUS. This result shows that the 332-121 bp region is enough and essential for the petal specificity and the InMYB1_332-121b×3_TATA_Ω could be used for the molecular breeding of floricultural crops.
{"title":"Dissecting promoter of InMYB1 gene showing petal-specific expression.","authors":"Mirai Azuma, Y. Oshima, Shingo Sakamoto, Nobutaka Mitsuda, M. Ohme-Takagi, S. Otagaki, S. Matsumoto, K. Shiratake","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0529A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0529A","url":null,"abstract":"We had previously reported that the InMYB1 promoter, the 1023 bp upstream region of InMYB1, works petal-specifically in various dicot plants by recognizing petal identity at a cellular level. To determine the petal-specific region in the InMYB1 promoter, Arabidopsis plants harboring InMYB1_1023b::GUS (β-glucuronidase), InMYB1_713b::GUS, InMYB1_506b::GUS, InMYB1_403b::GUS, InMYB1_332b::GUS, InMYB1_200b::GUS and InMYB1_140b::GUS were produced and confirmed a shortest region, which has the petal-specific promoter activity by using histochemical GUS assay. Petal-specific GUS staining was not observed in the Arabidopsis plants transformed with InMYB1_200b::GUS and InMYB1_140b::GUS, but observed in transgenic Arabidopsis plants harboring from InMYB1_1023b::GUS to InMYB1_332b::GUS. cDNA sequence of InMYB1 shows that 120 bp upstream region of InMYB1 is 5' untranslated region, suggesting that the 332-121 bp upstream region of InMYB1 contains an important element for petal-specific gene expression. In the Arabidopsis harboring the InMYB1_332-121b×3_TATA_Ω::GUS, petal-specific GUS staining was observed and the staining was stronger than in the Arabidopsis harboring InMYB1_1023b::GUS. This result shows that the 332-121 bp region is enough and essential for the petal specificity and the InMYB1_332-121b×3_TATA_Ω could be used for the molecular breeding of floricultural crops.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"243-248"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0529A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48944190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-25DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0424A
Kanna Sato-Izawa, Kyoko Tokue, H. Ezura
Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.
{"title":"Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430.","authors":"Kanna Sato-Izawa, Kyoko Tokue, H. Ezura","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0424A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0424A","url":null,"abstract":"Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 2 1","pages":"181-185"},"PeriodicalIF":1.6,"publicationDate":"2018-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0424A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44088874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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