Pub Date : 2017-01-31DOI: 10.18681/PJN.V35.I01.P65-71
A. M. Rahoo
{"title":"Infection of Galleria mellonella larvae by Steinernema affine and production of infective juveniles","authors":"A. M. Rahoo","doi":"10.18681/PJN.V35.I01.P65-71","DOIUrl":"https://doi.org/10.18681/PJN.V35.I01.P65-71","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122115486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-31DOI: 10.18681/PJN.V35.I01.P85-92
A. Saad
{"title":"Evaluation of some non-fumigant nematicides and the biocide avermactin for managing Meloidogyne incognita in tomatoes","authors":"A. Saad","doi":"10.18681/PJN.V35.I01.P85-92","DOIUrl":"https://doi.org/10.18681/PJN.V35.I01.P85-92","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126222434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-31DOI: 10.18681/PJN.V35.I01.P37-45
E. Mahdikhani-Moghadam
The survey of soil nematodes of the West Azarbaijan province of Iran revealed the presence of predatory nematodes of the order Mononchida. A total of 36 soil samples were collected in the summer of 2014 from the rhizosphere of stone fruits in different areas of the province. Soil analysis results in the identification of 5 species of mononchid nematodes viz., Anatonchus kashmirensis, A. tridentatus, Clarkus papillatus, Mylonchulus brachyuris and M. paitensis. Among them, A. kashmirensis is recorded for the first time in Iran.
{"title":"Study of some mononchids (Nematoda: Mononchida) from Iran","authors":"E. Mahdikhani-Moghadam","doi":"10.18681/PJN.V35.I01.P37-45","DOIUrl":"https://doi.org/10.18681/PJN.V35.I01.P37-45","url":null,"abstract":"The survey of soil nematodes of the West Azarbaijan province of Iran revealed the presence of predatory nematodes of the order Mononchida. A total of 36 soil samples were collected in the summer of 2014 from the rhizosphere of stone fruits in different areas of the province. Soil analysis results in the identification of 5 species of mononchid nematodes viz., Anatonchus kashmirensis, A. tridentatus, Clarkus papillatus, Mylonchulus brachyuris and M. paitensis. Among them, A. kashmirensis is recorded for the first time in Iran.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117281689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-31DOI: 10.18681/PJN.V35.I01.P73-78
M. S. Bajestani
In this study, the inhibitory effect of medicinal plant extracts viz., marigold (Tagetes spp.), rosemary (Rosmarinus officinalis L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000 juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the nematode inoculum increased, reproduction factor also increased while extract treatments effect decreased. In between treatments, rosemary extract has the greatest impact on the reduction of nematode populations @ 40% concentration as compared to control.
{"title":"Effect of medicinal plant extracts on inoculated Meloidogyne javanica in tomato","authors":"M. S. Bajestani","doi":"10.18681/PJN.V35.I01.P73-78","DOIUrl":"https://doi.org/10.18681/PJN.V35.I01.P73-78","url":null,"abstract":"In this study, the inhibitory effect of medicinal plant extracts viz., marigold (Tagetes spp.), rosemary (Rosmarinus officinalis L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000 juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the nematode inoculum increased, reproduction factor also increased while extract treatments effect decreased. In between treatments, rosemary extract has the greatest impact on the reduction of nematode populations @ 40% concentration as compared to control.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129359019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P163
I. Ibrahim
A nematode survey was conducted in northern Egypt and a total of 240 soil and root samples were collected from the rhizosphere of the surveyed plants. Twenty-three genera of phytoparasitic nematodes were detected in the collected soil and root samples. In soil samples from Alexandria governorate, the sugar beet cyst nematode (Heterodera schachtii) was very common on sugar beet while the root-knot nematodes Meloidogyne incognita and M. javanica were very common on guava, olive trees and sugar beet. Helicotylenchus pseudorobustus, M. incognita, Pratylenchus sp., Rotylenchulus reniformis and Xiphinema sp. were observed in spearmint soil samples. The dagger nematode Xiphinema rivesi was found in orange soil samples from El-Nobarria, El-Beheira governorate. In lantana soil samples from El-Giza governorate, Aglenchus geraerti, Bitylenchus ventrosignatus, Coslenchus capsici, Helicotylenchus indicus and Malenchus bryanti were identified for the first time in Egypt. Survey results revealed new host plant records for most of the identified nematode species in Egypt.
{"title":"Occurrence of phytoparasitic nematodes on some crop plants in northern Egypt","authors":"I. Ibrahim","doi":"10.18681/PJN.V34.I02.P163","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P163","url":null,"abstract":"A nematode survey was conducted in northern Egypt and a total of 240 soil and root samples were collected from the rhizosphere of the surveyed plants. Twenty-three genera of phytoparasitic nematodes were detected in the collected soil and root samples. In soil samples from Alexandria governorate, the sugar beet cyst nematode (Heterodera schachtii) was very common on sugar beet while the root-knot nematodes Meloidogyne incognita and M. javanica were very common on guava, olive trees and sugar beet. Helicotylenchus pseudorobustus, M. incognita, Pratylenchus sp., Rotylenchulus reniformis and Xiphinema sp. were observed in spearmint soil samples. The dagger nematode Xiphinema rivesi was found in orange soil samples from El-Nobarria, El-Beheira governorate. In lantana soil samples from El-Giza governorate, Aglenchus geraerti, Bitylenchus ventrosignatus, Coslenchus capsici, Helicotylenchus indicus and Malenchus bryanti were identified for the first time in Egypt. Survey results revealed new host plant records for most of the identified nematode species in Egypt.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129003570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P193
Y. I. Erum, F. Shahina
Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.
{"title":"Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique","authors":"Y. I. Erum, F. Shahina","doi":"10.18681/PJN.V34.I02.P193","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P193","url":null,"abstract":"Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131206580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P205
A. Sattar
Current study was carried out in December, 2014 to investigate the occurrence of helminth parasites in catfish species belonging to family Ariidae (Blecker, 1862). Four species of catfishes namely Arius arius (Hamilton, 1822), Arius caelatus (Valenciennes, 1840), Arius dussumieri (Valenciennes, 1840) and Arius sona (Hamilton, 1822) off the Karachi coast were screened for the occurrence of helminth parasites. Fish were examined after washing contents of gastrointestinal tract and observed under light microscope with the help of regular parasitological methods. The nematode parasites namely; Raphidascaris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor, 1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been reported from Arius arius species in the region, off the Karachi coast
{"title":"Occurrence of some nematode parasites in the gastrointestinal tract of Ariidae (Teleostei: Siluriformes) catfish, Arius arius (Hamilton, 1822) from Karachi coast","authors":"A. Sattar","doi":"10.18681/PJN.V34.I02.P205","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P205","url":null,"abstract":"Current study was carried out in December, 2014 to investigate the occurrence of helminth parasites in catfish species belonging to family Ariidae (Blecker, 1862). Four species of catfishes namely Arius arius (Hamilton, 1822), Arius caelatus (Valenciennes, 1840), Arius dussumieri (Valenciennes, 1840) and Arius sona (Hamilton, 1822) off the Karachi coast were screened for the occurrence of helminth parasites. Fish were examined after washing contents of gastrointestinal tract and observed under light microscope with the help of regular parasitological methods. The nematode parasites namely; Raphidascaris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor, 1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been reported from Arius arius species in the region, off the Karachi coast","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122769280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P109
K. Tabassum, F. Shahina, K. Nasira, Y. I. Erum
Six new species of the genus Oscheius Andrassy, 1976 viz., Oscheius citri n. sp., O. cynodonti n. sp., O. cobbi n. sp., O. esculentus n. sp., O. punctata n. sp. and O. sacchari n. sp., are described by both morphological and molecular means from different agro-climatic regions of Sindh, Punjab and Azad Jammu & Kashmir, Pakistan. All species belong to insectivora group on the basis of leptoderan bursa, crochet needle-shaped spicules, normal rectum, lateral field with six separate lines and had unique ribosomal DNA-ITS, sequence. A compendium of the genus Oscheius (of both insectivora and dolichura groups) based on the following characters: body length, tail, spicules and gubernaculum length, ratios a, b, c, c' and vulva percentage is given. The morphometric and allometric characters with anterior and posterior regions were derived from the original descriptions. An identification key to 42 valid species of both insectivora and dolichura group of the genus Oscheius is given.
本文从形态和分子的角度描述了1976年在巴基斯坦信德省、旁遮普省和查谟和克什米尔不同农业气候区发现的6个新种:柑桔属(Oscheius citri n. sp)、cynodonti n. sp、cobbi n. sp、esculentus n. sp、punctata n. sp和sacchari n. sp。所有种属均属食虫纲，具有钩针状囊、正常直肠、6条分离线的侧野和独特的核糖体DNA-ITS序列。根据体长、尾长、针状体和管骨长度、比例A、b、c、c’和外阴百分率等特征，给出了食虫目和刺虫目双翅蝽属的概要。前、后区域的形态计量和异速计量特征来源于原始描述。给出了食虫科和刺虫科42种有效种的鉴定钥匙。
{"title":"Description of six new species of Oscheius Andrassy, 1976 (Nematoda: Rhabditida) from Pakistan with a key and diagnostic compendium to species of the genus","authors":"K. Tabassum, F. Shahina, K. Nasira, Y. I. Erum","doi":"10.18681/PJN.V34.I02.P109","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P109","url":null,"abstract":"Six new species of the genus Oscheius Andrassy, 1976 viz., Oscheius citri n. sp., O. cynodonti n. sp., O. cobbi n. sp., O. esculentus n. sp., O. punctata n. sp. and O. sacchari n. sp., are described by both morphological and molecular means from different agro-climatic regions of Sindh, Punjab and Azad Jammu & Kashmir, Pakistan. All species belong to insectivora group on the basis of leptoderan bursa, crochet needle-shaped spicules, normal rectum, lateral field with six separate lines and had unique ribosomal DNA-ITS, sequence. A compendium of the genus Oscheius (of both insectivora and dolichura groups) based on the following characters: body length, tail, spicules and gubernaculum length, ratios a, b, c, c' and vulva percentage is given. The morphometric and allometric characters with anterior and posterior regions were derived from the original descriptions. An identification key to 42 valid species of both insectivora and dolichura group of the genus Oscheius is given.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122152472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P183
M. Javeed
Two studies, in vitro and in the green house, were conducted to determine the efficacy of some Saudi Trichoderma isolates against Meloidogyne javanica. The isolates included: Trichoderma hamatum (one isolate), T. viride (one isolate), T. asperellum (three isolates), T. atroviride (one isolate) and T. harzianum (two isolates). In vitro , results showed that all eight tested isolates and their culture filtrates were effective at different levels, in egg hatch inhibition and mortality of juvenile (J 2 ). The mortality of J 2 and un-hatching of eggs increased as the concentrations of the culture filtrates increased. In general, T. harzianum (isolate No.27), T. hamatum (isolate No.5) and T. viride (isolate No.8) were the most effective among all tested isolates. Therefore, these three isolates were further evaluated in the greenhouse test on tomato against M. javanica . The three tested isolates inhibited the reproduction, root galling and number of juveniles of M. javanica. The T. harzianum (isolate No.27) was the most effective isolate.
{"title":"Antagonistic effects of some indigenous isolates of Trichoderma spp. against Meloidogyne javanica","authors":"M. Javeed","doi":"10.18681/PJN.V34.I02.P183","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P183","url":null,"abstract":"Two studies, in vitro and in the green house, were conducted to determine the efficacy of some Saudi Trichoderma isolates against Meloidogyne javanica. The isolates included: Trichoderma hamatum (one isolate), T. viride (one isolate), T. asperellum (three isolates), T. atroviride (one isolate) and T. harzianum (two isolates). In vitro , results showed that all eight tested isolates and their culture filtrates were effective at different levels, in egg hatch inhibition and mortality of juvenile (J 2 ). The mortality of J 2 and un-hatching of eggs increased as the concentrations of the culture filtrates increased. In general, T. harzianum (isolate No.27), T. hamatum (isolate No.5) and T. viride (isolate No.8) were the most effective among all tested isolates. Therefore, these three isolates were further evaluated in the greenhouse test on tomato against M. javanica . The three tested isolates inhibited the reproduction, root galling and number of juveniles of M. javanica. The T. harzianum (isolate No.27) was the most effective isolate.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115303754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-31DOI: 10.18681/PJN.V34.I02.P177
I. Ibrahim, M. El-Saedy, S. Awd-Allah, Z. Handoo
The interactions of the cyst nematodes Heterodera daverti, H. goldeni and H. zeae with the root-knot nematode Meloidogyne incognita on rice (Oryza sativa) cultivars 'Giza 178' and Sakha 101' were studied in the greenhouse. Inoculation with H. goldeni alone or one week before inoculation with M. incognita on rice cv. Sakha 101 resulted in a significant increase in the number of cysts of H. goldeni as compared to plants inoculated with M. incognita concurrently or a week beforehand. When H. daverti or H. zeae were inoculated one week after inoculation with M. incognita on rice cultivars Giza 178 or Sakha 101, respectively, the final population of these cyst nematodes increased. Treatments with M. incognita alone or one week before inoculations with the tested cyst nematodes induced a significant increase in the numbers of M. incognita root galls and egg-masses as compared to other treatments.
{"title":"Interactions of Heterodera daverti, H. goldeni and H. zeae with Meloidogyne incognita on rice","authors":"I. Ibrahim, M. El-Saedy, S. Awd-Allah, Z. Handoo","doi":"10.18681/PJN.V34.I02.P177","DOIUrl":"https://doi.org/10.18681/PJN.V34.I02.P177","url":null,"abstract":"The interactions of the cyst nematodes Heterodera daverti, H. goldeni and H. zeae with the root-knot nematode Meloidogyne incognita on rice (Oryza sativa) cultivars 'Giza 178' and Sakha 101' were studied in the greenhouse. Inoculation with H. goldeni alone or one week before inoculation with M. incognita on rice cv. Sakha 101 resulted in a significant increase in the number of cysts of H. goldeni as compared to plants inoculated with M. incognita concurrently or a week beforehand. When H. daverti or H. zeae were inoculated one week after inoculation with M. incognita on rice cultivars Giza 178 or Sakha 101, respectively, the final population of these cyst nematodes increased. Treatments with M. incognita alone or one week before inoculations with the tested cyst nematodes induced a significant increase in the numbers of M. incognita root galls and egg-masses as compared to other treatments.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122445551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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