E. Abdeen, H. Hussien, Zakariea Hussan, W. Abdella
{"title":"Genotyping and Virulence Genes of Enterococcus faecalis Isolated Form Kareish Cheese and Minced Meat in Egypt","authors":"E. Abdeen, H. Hussien, Zakariea Hussan, W. Abdella","doi":"10.3923/JM.2016.133.138","DOIUrl":"https://doi.org/10.3923/JM.2016.133.138","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87408547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Djauhari, Widanarni, Sukenda, M. Suprayudi, M. Zairin
{"title":"Characterization of Bacillus sp. NP5 and its Application as Probiotic for Common Carp (Cyprinus carpio)","authors":"R. Djauhari, Widanarni, Sukenda, M. Suprayudi, M. Zairin","doi":"10.3923/JM.2016.101.111","DOIUrl":"https://doi.org/10.3923/JM.2016.101.111","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77174673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sadiki, S. Abed, H. Barkai, M. Balouiri, F. E. Bergadi, S. I. Koraichi
{"title":"Plant Extracts Effect on the Cell Fungal Surface Hydrophobicity and Acid-base Properties","authors":"M. Sadiki, S. Abed, H. Barkai, M. Balouiri, F. E. Bergadi, S. I. Koraichi","doi":"10.3923/JM.2016.139.145","DOIUrl":"https://doi.org/10.3923/JM.2016.139.145","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83264777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Bitter Leaf Extract (Vernonia amygdalina) on Culturable Microorganisms Isolated from Palm Wine in Makurdi Metropolis","authors":"G. Gberikon, T. Ichor, Enyi Theresa Omeche","doi":"10.3923/JM.2016.112.118","DOIUrl":"https://doi.org/10.3923/JM.2016.112.118","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86550834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chitinases are hydrolytic enzymes that break down the glycosidic bonds in chitin. Chitin is a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), therefore, chitinases are generally found in organisms that either needs to reshape their own chitin or dissolve and digest the chitin of fungi or animals. The importance of chitinase in industries cannot be overemphasized as it has been applied in agriculture, as a biopesticide for control of plant fungi infections, in medicine, as indicators of fungi infection and in waste management, for biodegradation of fish waste. African catfish (Clarias gariepinus) which plays host to bacteria is very readily available and easy to cultivate thus providing a cheap means of obtaining chitinolytic bacteria for the production of chitinase in commercial quantity. Bacteria populations isolated from the skin and gut of catfish were screened on colloidal-chitin agar medium. Chitinase production was determined by zones of hydrolysis produced after 96 h of incubation at 37EC. The result of this investigation revealed thirty-six pure bacterial isolates from the skin and gut of catfish. Gram staining test revealed, twenty five Gram positive bacteria while eleven were Gram negative. After four days of incubation, twenty-six bacteria isolates obtained from the gut and skin of catfish were selected as chitinase producing bacteria based on the clear zones of hydrolysis produced. The bacterial isolates obtained will be very useful for the production of chitinase which can be employed for the biocontrol of fungal pathogens and harmful insects. This study presents a first time report of Chitinase producing Bacillus cereus from the gut of catfish (Clarias gariepinus).
{"title":"Isolation and Characterization of Chitinolytic Bacteria for Chitinase Production from the African Catfish, Clarias gariepinus (Burchell, 1822)","authors":"A. Ajayi, E. Onibokun, F. George, O. Atolagbe","doi":"10.3923/JM.2016.119.125","DOIUrl":"https://doi.org/10.3923/JM.2016.119.125","url":null,"abstract":"Chitinases are hydrolytic enzymes that break down the glycosidic bonds in chitin. Chitin is a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), therefore, chitinases are generally found in organisms that either needs to reshape their own chitin or dissolve and digest the chitin of fungi or animals. The importance of chitinase in industries cannot be overemphasized as it has been applied in agriculture, as a biopesticide for control of plant fungi infections, in medicine, as indicators of fungi infection and in waste management, for biodegradation of fish waste. African catfish (Clarias gariepinus) which plays host to bacteria is very readily available and easy to cultivate thus providing a cheap means of obtaining chitinolytic bacteria for the production of chitinase in commercial quantity. Bacteria populations isolated from the skin and gut of catfish were screened on colloidal-chitin agar medium. Chitinase production was determined by zones of hydrolysis produced after 96 h of incubation at 37EC. The result of this investigation revealed thirty-six pure bacterial isolates from the skin and gut of catfish. Gram staining test revealed, twenty five Gram positive bacteria while eleven were Gram negative. After four days of incubation, twenty-six bacteria isolates obtained from the gut and skin of catfish were selected as chitinase producing bacteria based on the clear zones of hydrolysis produced. The bacterial isolates obtained will be very useful for the production of chitinase which can be employed for the biocontrol of fungal pathogens and harmful insects. This study presents a first time report of Chitinase producing Bacillus cereus from the gut of catfish (Clarias gariepinus).","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90388443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Garba, W. Latip, M. Ali, Siti Nurbaya Oslan, R. Rahman
Unsaturated Fatty Acids (UFAs) play a vital role in membrane lipids fluidity which maintains its structural integrity and also, are nutritionally and pharmaceutically important. Fish and vegetable oils are the major sources of the so-called essential fatty acids required for normal physiological activities of the body. However, the increasing demands for these fatty acids require more reliable, flexible and cheaper alternative sources. Antarctic bacteria can produce large amounts of unsaturated fatty acids which can be enhanced using simple biotechnological tools. To achieve this, five isolates of Antarctic bacteria were grown at low temperature and analysed for cellular fatty acids using Gas Chromatography Mass Spectrometry (GCMS). The results obtained revealed high amount of unsaturated fatty acids in virtually all the bacteria. Highest amount was recorded in Arthrobacter sp., 3B (47.24%) followed by Pseudomonas sp., A8 (45.09%), Pseudomonas sp., A3 (33.17%) and Arthrobacter sp., PB (31.92%). The results obtained suggest that the bacteria could be used to enhance essential fatty acids production through metabolic engineering for industrial applications.
{"title":"Unsaturated fatty acids in antarctic bacteria","authors":"L. Garba, W. Latip, M. Ali, Siti Nurbaya Oslan, R. Rahman","doi":"10.3923/JM.2016.146.152","DOIUrl":"https://doi.org/10.3923/JM.2016.146.152","url":null,"abstract":"Unsaturated Fatty Acids (UFAs) play a vital role in membrane lipids fluidity which maintains its structural integrity and also, are nutritionally and pharmaceutically important. Fish and vegetable oils are the major sources of the so-called essential fatty acids required for normal physiological activities of the body. However, the increasing demands for these fatty acids require more reliable, flexible and cheaper alternative sources. Antarctic bacteria can produce large amounts of unsaturated fatty acids which can be enhanced using simple biotechnological tools. To achieve this, five isolates of Antarctic bacteria were grown at low temperature and analysed for cellular fatty acids using Gas Chromatography Mass Spectrometry (GCMS). The results obtained revealed high amount of unsaturated fatty acids in virtually all the bacteria. Highest amount was recorded in Arthrobacter sp., 3B (47.24%) followed by Pseudomonas sp., A8 (45.09%), Pseudomonas sp., A3 (33.17%) and Arthrobacter sp., PB (31.92%). The results obtained suggest that the bacteria could be used to enhance essential fatty acids production through metabolic engineering for industrial applications.","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88191915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Hidayati, A. Wahyudi, A. Suwanto, R. Widyastuti
{"title":"In planta Screening of Rhizobacterial Community for Promoting Maize (Zea mays cv. BISI 2) Growth in Dryland Agriculture","authors":"E. Hidayati, A. Wahyudi, A. Suwanto, R. Widyastuti","doi":"10.3923/JM.2016.70.79","DOIUrl":"https://doi.org/10.3923/JM.2016.70.79","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81129154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Alternative Antibiotics Source from Symbiont of Lactid Acid Bacteria Inside Stomach of Honeybees (Apis mellifera and Apis dorsata) Against Multiresistant Antibiotics Pathogenic Bacteria","authors":"N. A. Sandi, S. I. Salasia","doi":"10.3923/JM.2016.93.100","DOIUrl":"https://doi.org/10.3923/JM.2016.93.100","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82603060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Varying Storage Temperatures on the Microbiological Quality of Street Sold Water in the Kumasi Metropolis, Ghana","authors":"M. Addo, C. Woods, L. A. Andoh, K. Obiri-Dans","doi":"10.3923/JM.2016.56.63","DOIUrl":"https://doi.org/10.3923/JM.2016.56.63","url":null,"abstract":"","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84988885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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