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Clinical data-based modeling of IVF live birth outcome and its application. 基于临床数据的试管婴儿活产结果建模及其应用。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-07-08 DOI: 10.1186/s12958-024-01253-3
Liu Liu, Hua Liang, Jing Yang, Fujin Shen, Jiao Chen, Liangfei Ao

Background: The low live birth rate and difficult decision-making of the in vitro fertilization (IVF) treatment regimen bring great trouble to patients and clinicians. Based on the retrospective clinical data of patients undergoing the IVF cycle, this study aims to establish classification models for predicting live birth outcome (LBO) with machine learning methods.

Methods: The historical data of a total of 1405 patients undergoing IVF cycle were first collected and then analyzed by univariate and multivariate analysis. The statistically significant factors were identified and taken as input to build the artificial neural network (ANN) model and supporting vector machine (SVM) model for predicting the LBO. By comparing the model performance, the one with better results was selected as the final prediction model and applied in real clinical applications.

Results: Univariate and multivariate analysis shows that 7 factors were closely related to the LBO (with P < 0.05): Age, ovarian sensitivity index (OSI), controlled ovarian stimulation (COS) treatment regimen, Gn starting dose, endometrial thickness on human chorionic gonadotrophin (HCG) day, Progesterone (P) value on HCG day, and embryo transfer strategy. By taking the 7 factors as input, the ANN-based and SVM-based LBO models were established, yielding good prediction performance. Compared with the ANN model, the SVM model performs much better and was selected as the final model for the LBO prediction. In real clinical applications, the proposed ANN-based LBO model can predict the LBO with good performance and recommend the embryo transfer strategy of potential good LBO.

Conclusions: The proposed model involving all essential IVF treatment factors can accurately predict LBO. It can provide objective and scientific assistance to clinicians for customizing the IVF treatment strategy like the embryo transfer strategy.

背景:低活产率和体外受精(IVF)治疗方案决策困难给患者和临床医生带来了极大的困扰。本研究以接受试管婴儿周期治疗的患者的回顾性临床数据为基础,旨在利用机器学习方法建立预测活产结果(LBO)的分类模型:方法:首先收集了1405名接受试管婴儿周期的患者的历史数据,然后通过单变量和多变量分析进行分析。方法:首先收集了1405名试管婴儿周期患者的历史数据,然后通过单变量和多变量分析对这些数据进行了分析,找出了在统计上具有重要意义的因素,并将其作为建立人工神经网络(ANN)模型和支持向量机(SVM)模型的输入,以预测LBO。通过比较模型的性能,选择结果更好的模型作为最终预测模型,并应用于实际临床应用中:结果:单变量和多变量分析表明,7个因素与LBO密切相关(附P结论):所提出的模型涉及所有重要的试管婴儿治疗因素,可以准确预测 LBO。它可以为临床医生定制胚胎移植策略等试管婴儿治疗策略提供客观、科学的帮助。
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引用次数: 0
DNA methylation landscape in pregnancy-induced hypertension: progress and challenges. 妊娠诱发高血压的 DNA 甲基化状况:进展与挑战。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-07-08 DOI: 10.1186/s12958-024-01248-0
Fengying Deng, Jiahui Lei, Junlan Qiu, Chenxuan Zhao, Xietong Wang, Min Li, Miao Sun, Meihua Zhang, Qinqin Gao

Gestational hypertension (PIH), especially pre-eclampsia (PE), is a common complication of pregnancy. This condition poses significant risks to the health of both the mother and the fetus. Emerging evidence suggests that epigenetic modifications, particularly DNA methylation, may play a role in initiating the earliest pathophysiology of PIH. This article describes the relationship between DNA methylation and placental trophoblast function, genes associated with the placental microenvironment, the placental vascular system, and maternal blood and vascular function, abnormalities of umbilical cord blood and vascular function in the onset and progression of PIH, as well as changes in DNA methylation in the progeny of PIH, in terms of maternal, fetal, and offspring. We also explore the latest research on DNA methylation-based early detection, diagnosis and potential therapeutic strategies for PIH. This will enable the field of DNA methylation research to continue to enhance our understanding of the epigenetic regulation of PIH genes and identify potential therapeutic targets.

妊娠高血压(PIH),尤其是子痫前期(PE),是一种常见的妊娠并发症。这种情况对母亲和胎儿的健康都有很大风险。新的证据表明,表观遗传修饰,尤其是 DNA 甲基化,可能在引发 PIH 最早的病理生理学过程中发挥作用。本文介绍了 DNA 甲基化与胎盘滋养层功能的关系,与胎盘微环境、胎盘血管系统、母体血液和血管功能相关的基因,PIH 发病和进展过程中脐带血和血管功能的异常,以及 PIH 后代(母体、胎儿和后代)DNA 甲基化的变化。我们还将探讨基于 DNA 甲基化的 PIH 早期检测、诊断和潜在治疗策略的最新研究。这将使 DNA 甲基化研究领域继续增进我们对 PIH 基因表观遗传调控的了解,并确定潜在的治疗目标。
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引用次数: 0
9-cis-retinoic acid signaling in Sertoli cells regulates their immunomodulatory function to control lymphocyte physiology and Treg differentiation. Sertoli细胞中的9-顺式维甲酸信号调节其免疫调节功能,从而控制淋巴细胞的生理学和Treg分化。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-26 DOI: 10.1186/s12958-024-01246-2
Alicja Kamińska, Laura Pardyak, Sylwia Lustofin, Karolina Gielata, Zbigniew Arent, Agnieszka Pietsch-Fulbiszewska, Anna Hejmej

Background: Testis is an immune privileged organ, which prevents the immune response against sperm antigens and inflammation. Testicular cells responsible for immune tolerance are mainly Sertoli cells, which form the blood-testis barrier and produce immunosuppressive factors. Sertoli cells prevent inflammation in the testis and maintain immune tolerance by inhibiting proliferation and inducing lymphocyte apoptosis. It has been shown that 9-cis-retinoic acid (9cRA) blocks ex vivo apoptosis of peripheral blood lymphocytes and promotes the differentiation of Treg cells in the gut. However, the role of retinoid signaling in regulating the immune privilege of the testes remains unknown.

Objective: The aim of this study was to determine whether 9cRA, acting via the retinoic acid receptors (RAR) and the retinoic X receptors (RXR), controls the immunomodulatory functions of Sertoli cells by influencing the secretion of anti-inflammatory/pro-inflammatory factors, lymphocyte physiology and Treg cell differentiation.

Methods: Experiments were performed using in vitro model of co-cultures of murine Sertoli cells and T lymphocytes. Agonists and antagonists of retinoic acid receptors were used to inhibit/stimulate retinoid signaling in Sertoli cells.

Results: Our results have demonstrated that 9cRA inhibits the expression of immunosuppressive genes and enhances the expression of pro-inflammatory factors in Sertoli cells and lymphocytes, increases lymphocyte viability and decreases apoptosis rate. Moreover, we have found that 9cRA blocks lymphocyte apoptosis acting through both RAR and RXR and inhibiting FasL/Fas/Caspase 8 and Bax/Bcl-2/Caspase 9 pathways. Finally, we have shown that 9cRA signaling in Sertoli cells inhibits Treg differentiation.

Conclusion: Collectively, our results indicate that retinoid signaling negatively regulates immunologically privileged functions of Sertoli cells, crucial for ensuring male fertility. 9cRA inhibits lymphocyte apoptosis, which can be related to the development of autoimmunity, inflammation, and, in consequence, infertility.

背景:睾丸是一个免疫特权器官,可防止针对精子抗原和炎症的免疫反应。负责免疫耐受的睾丸细胞主要是 Sertoli 细胞,它们构成血液-睾丸屏障并产生免疫抑制因子。Sertoli 细胞通过抑制增殖和诱导淋巴细胞凋亡来防止睾丸发炎并维持免疫耐受。研究表明,9-顺式维甲酸(9cRA)可阻止外周血淋巴细胞的体内凋亡,并促进肠道中 Treg 细胞的分化。然而,视黄酸信号在调节睾丸免疫特权中的作用仍然未知:本研究旨在确定9cRA是否通过视黄酸受体(RAR)和视黄酸X受体(RXR)发挥作用,通过影响抗炎/促炎因子的分泌、淋巴细胞生理机能和Treg细胞分化来控制Sertoli细胞的免疫调节功能:实验采用小鼠 Sertoli 细胞和 T 淋巴细胞共培养的体外模型。方法:实验采用小鼠 Sertoli 细胞和 T 淋巴细胞共同培养的体外模型,使用维甲酸受体的激动剂和拮抗剂来抑制/刺激 Sertoli 细胞中的维甲酸信号转导:结果:我们的研究结果表明,9cRA 能抑制 Sertoli 细胞和淋巴细胞中免疫抑制基因的表达,增强促炎因子的表达,提高淋巴细胞的活力,降低细胞凋亡率。此外,我们还发现 9cRA 可通过 RAR 和 RXR 阻断淋巴细胞凋亡,抑制 FasL/Fas/Caspase 8 和 Bax/Bcl-2/Caspase 9 通路。最后,我们还发现 9cRA 信号在 Sertoli 细胞中可抑制 Treg 分化:总之,我们的研究结果表明,视黄醇信号负向调节 Sertoli 细胞的免疫特权功能,这对确保男性生育能力至关重要。9cRA抑制淋巴细胞凋亡,这可能与自身免疫、炎症以及不育的发展有关。
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引用次数: 0
LncRNA MALAT1 facilitates BM-MSCs differentiation into endothelial cells and ameliorates erectile dysfunction via the miR-206/CDC42/PAK1/paxillin signalling axis. LncRNA MALAT1通过miR-206/CDC42/PAK1/paxillin信号轴促进BM-间充质干细胞分化为内皮细胞并改善勃起功能障碍。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-25 DOI: 10.1186/s12958-024-01240-8
Longhua Luo, Zixin Wang, Xuxian Tong, Tenxian Xiong, Minggen Chen, Xiang Liu, Cong Peng, Xiang Sun

Background: Erectile dysfunction (ED) is a common male sexual dysfunction, with an increasing incidence, and the current treatment is often ineffective.

Methods: Vascular endothelial growth factor (VEGFA) was used to treat bone marrow-derived mesenchymal stem cells (BM-MSCs), and their cell migration rates were determined by Transwell assays. The expression of the von Willebrand Factor (vWF)VE-cadherin, and endothelial nitric oxide synthase(eNOS) endothelial markers was determined by qRT‒PCR and Western blot analyses. The MALAT1-induced differentiation of BM-MCs to ECs via the CDC42/PAK1/paxillin pathway was explored by transfecting VEGFA-induced BM-MSC with si-MALAT1 and overexpressing CDC42 and PAK1. The binding capacity between CDC42, PAK1, and paxillin in VEGFA-treated and non-VEGFA-treated BM-MSCs was examined by protein immunoprecipitation. MiR-206 was overexpressed in VEGFA-induced BM-MSC, and the binding sites of MALAT1, miR-206, and CDC42 were identified using a luciferase assay. Sixty male Sprague‒Dawley rats were divided into six groups (n = 10/group). DMED modelling was demonstrated by APO experiments and was assessed by measuring blood glucose levels. Erectile function was assessed by measuring the intracavernosa pressure (ICP) and mean arterial pressure (MAP). Penile erectile tissue was analysed by qRT‒PCR, Western blot analysis, and immunohistochemical staining.

Results: MALAT1 under VEGFA treatment conditions regulates the differentiation of BM-MSCs into ECs by modulating the CDC42/PAK1/paxillin axis. In vitro experiments demonstrated that interference with CDC42 and MALAT1 expression inhibited the differentiation of BM-MSCs to ECs. CDC42 binds to PAK1, and PAK1 binds to paxillin. In addition, CDC42 in the VEGFA group had a greater ability to bind to PAK1, whereas PAK1 in the VEGFA group had a greater ability to bind to paxillin. Overexpression of miR-206 in VEGFA-induced BM-MSCs demonstrated that MALAT1 competes with the CDC42 3'-UTR for binding to miR-206, which in turn is involved in the differentiation of BM-MSCs to ECs. Compared to the DMED model group, the ICP/MAP ratio was significantly greater in the three BM-MSCs treatment groups.

Conclusions: MALAT1 facilitates BM-MSC differentiation into ECs by regulating the miR-206/CDC42/PAK1/paxillin axis to improve ED. The present findings revealed the vital role of MALAT1 in the repair of BM-MSCs for erectile function and provided new mechanistic insights into the BM-MSC-mediated repair of DMED.

背景:勃起功能障碍(ED)是一种常见的男性性功能障碍,发病率越来越高,目前的治疗方法往往效果不佳:方法:用血管内皮生长因子(VEGFA)处理骨髓间充质干细胞(BM-MSCs),并通过 Transwell 试验测定其细胞迁移率。通过qRT-PCR和Western印迹分析确定了von Willebrand因子(vWF)VE-cadherin和内皮一氧化氮合酶(eNOS)内皮标志物的表达。通过用si-MALAT1转染VEGFA诱导的BM-MSC并过表达CDC42和PAK1,探讨了MALAT1通过CDC42/PAK1/paxillin途径诱导BM-MSC分化为ECs。蛋白免疫沉淀法检测了VEGFA处理和非VEGFA处理的BM-MSCs中CDC42、PAK1和paxillin之间的结合能力。MiR-206 在 VEGFA 诱导的 BM-MSC 中过表达,并通过荧光素酶试验确定了 MALAT1、miR-206 和 CDC42 的结合位点。60 只雄性 Sprague-Dawley 大鼠被分为 6 组(n = 10/组)。DMED模型由APO实验证明,并通过测量血糖水平进行评估。勃起功能通过测量阴茎海绵体内压(ICP)和平均动脉压(MAP)进行评估。阴茎勃起组织通过 qRT-PCR、Western 印迹分析和免疫组化染色进行分析:结果:在VEGFA处理条件下,MALAT1通过调节CDC42/PAK1/paxillin轴来调节BM-间充质干细胞向ECs的分化。体外实验表明,干扰 CDC42 和 MALAT1 的表达会抑制 BM-MSCs 向 EC 的分化。CDC42 与 PAK1 结合,而 PAK1 与 paxillin 结合。此外,VEGFA 组的 CDC42 与 PAK1 结合的能力更强,而 VEGFA 组的 PAK1 与 paxillin 结合的能力更强。在VEGFA诱导的BM-MSCs中过表达miR-206表明,MALAT1与CDC42 3'-UTR竞争结合miR-206,而miR-206又参与了BM-MSCs向ECs的分化。与DMED模型组相比,三个BM-MSCs处理组的ICP/MAP比值明显增大:结论:MALAT1通过调控miR-206/CDC42/PAK1/paxillin轴促进BM-间充质干细胞分化为EC,从而改善ED。本研究结果揭示了MALAT1在BM-间充质干细胞修复勃起功能中的重要作用,并为BM-间充质干细胞介导的DMED修复提供了新的机制认识。
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引用次数: 0
Potential molecular mechanisms and clinical implications of piRNAs in preeclampsia: a review. 子痫前期 piRNAs 的潜在分子机制和临床意义:综述。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-24 DOI: 10.1186/s12958-024-01247-1
Yuanxuan Ma, Bo Hou, Jinbao Zong, Shiguo Liu

Preeclampsia is a multisystem progressive condition and is one of the most serious complications of pregnancy. Owing to its unclear pathogenesis, there are no precise and effective therapeutic targets for preeclampsia, and the only available treatment strategy is to terminate the pregnancy and eliminate the clinical symptoms. In recent years, non-coding RNAs have become a hotspot in preeclampsia research and have shown promise as effective biomarkers for the early diagnosis of preeclampsia over conventional biochemical markers. PIWI-interacting RNAs, novel small non-coding RNA that interact with PIWI proteins, are involved in the pathogenesis of various diseases at the transcriptional or post-transcriptional level. However, the mechanisms underlying the role of PIWI-interacting RNAs in the pathogenesis of preeclampsia remain unclear. In this review, we discuss the findings of existing studies on PIWI-interacting RNA biogenesis, functions, and their possible roles in preeclampsia, providing novel insights into the potential application of PIWI-interacting RNAs in the early diagnosis and clinical treatment of preeclampsia.

子痫前期是一种多系统进展性疾病,是妊娠期最严重的并发症之一。由于子痫前期发病机制不清,目前尚无精确有效的治疗靶点,唯一的治疗策略是终止妊娠,消除临床症状。近年来,非编码 RNA 已成为子痫前期研究的热点,并有望成为比传统生化标志物更有效的早期诊断子痫前期的生物标志物。与 PIWI 蛋白相互作用的新型小非编码 RNA--PIWI-interacting RNA,在转录或转录后水平参与多种疾病的发病机制。然而,PIWI 相互作用 RNA 在子痫前期发病机制中的作用机制仍不清楚。在这篇综述中,我们讨论了有关 PIWI 相互作用 RNA 的生物发生、功能及其在子痫前期中的可能作用的现有研究结果,为 PIWI 相互作用 RNA 在子痫前期的早期诊断和临床治疗中的潜在应用提供了新的见解。
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引用次数: 0
Associations between a normal-range free thyroxine concentration and ovarian reserve in infertile women undergoing treatment via assisted reproductive technology. 接受辅助生殖技术治疗的不孕妇女体内正常范围的游离甲状腺素浓度与卵巢储备功能之间的关系。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-22 DOI: 10.1186/s12958-024-01226-6
Qiaoling Zhang, Dandan Zhang, Haoyuan Liu, Jinyun Fu, Li Tang, Meng Rao

Background: Some recent studies have shown that female subclinical hypothyroidism (SCH) is associated with diminished ovarian reserve (DOR). In this study, we aimed to investigate whether serum-free thyroxine (fT4) concentrations within the reference range are associated with ovarian reserve in women.

Methods: This cross-sectional study included 4933 infertile women with normal-range fT4 concentrations who received assisted reproductive technology treatment in our clinic. The data of women in different fT4 concentration tertiles (namely 12-15.33, 15.34-18.67, and 18.68-22 pmol/L) were compared with ovarian reserve markers, namely the anti-Müllerian hormone (AMH) concentration, the antral follicle count (AFC), and the number of aspirated oocytes. The primary outcomes were the AMH concentration and the risk of DOR, diagnosed as an AMH concentration < 1.1 ng/mL.

Results: The average ages of women in the low-normal, middle-normal, and high-normal fT4 tertiles were 33.20 (standard deviation [SD]: 5.11), 32.33 (SD: 5.13), and 31.61 (SD: 5.10) years, respectively (p < 0.0001). AMH concentrations (adjusted mean: 3.32 [95% confidence interval {CI}: 3.16 to 3.50] vs. 3.51 [3.40 to 3.62] vs. 3.64 [3.50 to 3.80] ng/mL, p = 0.022) were significantly different between the fT4 concentration tertiles. The risk of DOR was significantly increased in the low-normal (adjusted odds ratio: 1.61 [95% CI: 1.01 to 2.58]) and middle-normal (1.47 [95% CI: 1.00 to 2.16]) tertiles compared with the high-normal tertile. Subgroup analysis showed that AMH concentrations were significantly different among the fT4 concentration tertiles in women aged < 35 years (adjusted mean: 3.94 [95% CI: 3.70 to 4.20] vs. 4.25 [4.11 to 4.39] vs. 4.38 [4.18 to 4.58], p = 0.028), whereas this difference was not significant in women aged ≥ 35 years (p = 0.534). The general additive models using fT4 as a continuous variable indicated that a lower fT4 concentration within the normal range was significantly associated with a lower AMH concentration (p = 0.027), a lower AFC (p = 0.018), a lower number of aspirated oocytes (p = 0.001), and a higher risk of DOR (p = 0.007).

Conclusion: Low-normal fT4 concentrations are associated with lower ovarian reserve in infertile women.

背景:最近的一些研究表明,女性亚临床甲状腺功能减退症(SCH)与卵巢储备功能降低(DOR)有关。本研究旨在探讨血清游离甲状腺素(fT4)浓度在参考范围内是否与女性卵巢储备功能有关:这项横断面研究纳入了4933名fT4浓度在正常范围内的不孕妇女,她们都在本诊所接受了辅助生殖技术治疗。将不同fT4浓度三分位数(即12-15.33、15.34-18.67和18.68-22 pmol/L)妇女的数据与卵巢储备标志物(即抗穆勒氏管激素(AMH)浓度、前卵泡计数(AFC)和抽取的卵母细胞数)进行了比较。主要结果是 AMH 浓度和 DOR 风险(诊断为 AMH 浓度结果):低正常、中正常和高正常 fT4 三元组妇女的平均年龄分别为 33.20(标准差 [SD]:5.11)、32.33(标准差:5.13)和 31.61(标准差:5.10)岁(P 结论:低正常、中正常和高正常 fT4 三元组妇女的平均年龄为 33.20(标准差 [SD]:5.11)、32.33(标准差:5.13)和 31.61(标准差:5.10)岁:低正常 fT4 浓度与不孕妇女较低的卵巢储备功能有关。
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引用次数: 0
FMR1 allelic complexity in premutation carriers provides no evidence for a correlation with age at amenorrhea. 预突变携带者的 FMR1 等位基因复杂性没有证据表明与闭经年龄有关。
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-21 DOI: 10.1186/s12958-024-01227-5
Bárbara Rodrigues, Vanessa Sousa, Carolyn M Yrigollen, Flora Tassone, Olatz Villate, Emily G Allen, Anne Glicksman, Nicole Tortora, Sarah L Nolin, António J A Nogueira, Paula Jorge

Background: Premutations in the Fragile X Messenger Ribonucleoprotein 1 (FMR1) gene, defined as between 55 and 200 CGGs, have been implicated in fragile X-associated primary ovarian insufficiency (FXPOI). Only 20% of female premutation carriers develop early ovulatory dysfunction, the reason for this incomplete penetrance is unknown. This study validated the mathematical model in premutation alleles, after assigning each allele a score representing allelic complexity. Subsequently, allelic scores were used to investigate the impact of allele complexity on age at amenorrhea for 58 premutation cases (116 alleles) previously published.

Methods: The allelic score was determined using a formula previously described by our group. The impact of each allelic score on age at amenorrhea was analyzed using Pearson's test and a contour plot generated to visualize the effect.

Results: Correlation of allelic score revealed two distinct complexity behaviors in premutation alleles. No significant correlation was observed between the allelic score of premutation alleles and age at amenorrhea. The same lack of significant correlation was observed regarding normal-sized alleles, despite a nearly significant trend.

Conclusions: Our results suggest that the use of allelic scores combination have the potential to explain female infertility, namely the development of FXPOI, or ovarian dysfunction, despite the lack of correlation with age at amenorrhea. Such a finding is of great clinical significance for early identification of females at risk of ovulatory dysfunction, enhancement of fertility preservation techniques, and increasing the probability for a successful pregnancy in females with premutations. Additional investigation is necessary to validate this hypothesis.

背景:脆性 X 信使核糖核蛋白 1(FMR1)基因的突变(定义为 55 至 200 个 CGGs 之间)与脆性 X 相关性原发性卵巢功能不全(FXPOI)有关。只有 20% 的女性突变携带者会出现早期排卵功能障碍,而这种不完全渗透性的原因尚不清楚。这项研究在给每个等位基因分配一个代表等位基因复杂性的分数后,验证了预突变等位基因的数学模型。随后,等位基因得分被用于研究等位基因复杂性对闭经年龄的影响:等位基因得分是用我们小组之前描述的公式确定的。结果:等位基因得分与闭经年龄的相关性显示,等位基因得分与闭经年龄的相关性显示,等位基因得分与闭经年龄的相关性显示,等位基因得分与闭经年龄的相关性显示,等位基因得分与闭经年龄的相关性显示:结果:等位基因得分的相关性揭示了突变前等位基因的两种不同的复杂性行为。在前突变等位基因的等位基因得分和闭经年龄之间没有观察到明显的相关性。在正常大小等位基因方面,尽管有近乎显著的趋势,但同样没有观察到明显的相关性:我们的研究结果表明,使用等位基因评分组合有可能解释女性不孕症,即 FXPOI 或卵巢功能障碍的发生,尽管与闭经年龄缺乏相关性。这一发现对于早期识别有排卵功能障碍风险的女性、加强生育力保存技术以及提高早发型女性成功怀孕的概率具有重要的临床意义。要验证这一假设,还需要进一步的研究。
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引用次数: 0
The relationship between thyroid autoantibodies and X chromosome monosomy in the chorionic tissue of patients with missed miscarriage. 甲状腺自身抗体与漏诊流产患者绒毛组织中X染色体单体的关系
IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-20 DOI: 10.1186/s12958-024-01245-3
Lu Zhao, Li Liu, Hua Yang

Objective: The aim of this study was to investigate the relationship between thyroid autoantibodies (TGAb and TPOAb) and X chromosome monosomy in the chorionic tissue of patients with missed early miscarriage.

Methods: The baseline data, thyroid function, thyroid antibody and the chromosomes from the chorionic tissue of 228 patients with missed early miscarriage were examined.

Results: (1) Among the 228 patients, 121 had a normal chromosome number, and 107 had an abnormal chromosome number. The majority of them were autosomal trisomy, of which trisomy 16 (40.19%) was predominant. Sex chromosome monosomy (28.04%) was secondary. (2) Among the 228 patients, 208 patients in this study had normal thyroid function (including 134 cases of negative thyroid antibodies and 74 cases of positive thyroid antibodies alone); 6 patients had abnormal thyroid function (including 2 cases of clinical hyperthyroidism, 3 cases of subclinical hypothyroidism, 1 case of hypothyroxinemia); and 14 patients had normal TSH and elevated T4 alone.(3) After exclusion of patients with thyroid function abnormalities, there were no significant differences in baseline data between the normal chromosome group and the abnormal chromosome group (P > 0.05). However, there was a significant difference in TGAb and TPOAb between the normal chromosome and abnormal chromosome group with 45, X karyotype, with a higher proportion of TGAb and/or TPOAb positivity in the 45, X karyotype group (P < 0.05). Additionally, compared to TGAb and/or TPOAb-positive patients, the risk of X chromosome monosomy was significantly reduced in TGAb and TPOAb-negative patients (P < 0.05). Moreover, both TGAb and TPOAb titer values in the X chromosome monosomy group were higher than those in the chromosomally normal group (P < 0.05).

Conclusion: There is a correlation between TGAb, TPOAb and X chromosome monosomy in the chorionic tissue of patients with missed early miscarriage, although the mechanism remains to be further investigated.

研究目的本研究旨在探讨甲状腺自身抗体(TGAb和TPOAb)与早期流产漏诊患者绒毛组织中X染色体单体的关系:结果:(1)228例患者中,121例染色体数目正常,107例染色体数目异常。大多数为常染色体三体综合征,其中以 16 三体综合征(40.19%)为主。性染色体单体(28.04%)是次要的。(2)在228例患者中,208例患者甲状腺功能正常(包括134例甲状腺抗体阴性,74例单纯甲状腺抗体阳性);6例患者甲状腺功能异常(包括2例临床甲亢,3例亚临床甲减,1例甲状腺功能减退);14例患者促甲状腺激素正常,单纯T4升高。(3)排除甲状腺功能异常的患者后,染色体正常组与染色体异常组的基线数据无显著差异(P > 0.05)。然而,染色体正常组与染色体异常组之间的 TGAb 和 TPOAb 存在显著差异,45X 核型组的 TGAb 和/或 TPOAb 阳性比例更高(P 结论:TGAb 和/或 TPOAb 与甲状腺功能异常之间存在相关性:漏诊早期流产患者绒毛组织中的TGAb、TPOAb与X染色体单体存在相关性,但其机制仍有待进一步研究。
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引用次数: 0
The role of age and AMH on cumulative live birth rates over multiple frozen-thawed embryo transfer cycles: a study based on low prognosis patients of POSEIDON 3 and 4 groups. 年龄和 AMH 对多个冷冻-解冻胚胎移植周期累积活产率的影响:基于 POSEIDON 3 组和 4 组低预后患者的研究。
IF 4.4 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-17 DOI: 10.1186/s12958-024-01243-5
Lan Xia, Xiaowei Zhou, Xiaoling Wang, Shen Zhao, Xian Wu, Huihui Xu, Aijun Zhang, Zhihong Niu

Background: Among the POSEIDON criteria, group 3 and group 4 have an expected low prognosis. For those patients with inadequate ovary reserve, embryo accumulated from consecutive oocyte retrieval cycles for multiple frozen-thawed embryo transfers (FET) has become more common. It is necessary to inform them of the pregnancy outcomes after single or multiple FET cycles before the treatment. However few studies about cumulative live birth rate (CLBR) for those with low prognosis have been reported.

Methods: This retrospective study included 4712 patients undergoing frozen embryo transfer cycles from July 2015 to August 2020. Patients were stratified as POSEIDON group 3, group 4, control 1 group (< 35 years) and control 2 group (≥ 35 years). The primary outcome is CLBRs up to six FET cycles and the secondary outcomes were LBRs per transfer cycle. Optimistic approach was used for the analysis of CLBRs and the depiction of cumulative incidence curves.

Results: Under optimistic model analyses, control 1 group exhibited the highest CLBR (93.98%, 95%CI 91.63-95.67%) within 6 FET cycles, followed by the CLBR from women in POSEIDON group 3(92.51%, 95%CI 77.1-97.55)was slightly lower than that in control 1 group. The CLBR of POSEIDON group 4(55% ,95%CI 39.34-70.66%)was the lowest and significantly lower than that of control 2 group(88.7%, 95%CI 80.68-96.72%). Further, patients in POSEIDON group 4 reached a CLBR plateau after 5 FET cycles.

Conclusions: The patients of POSEIDON group 3 may not be considered as traditional "low prognosis" in clinical practice as extending the number of FET cycles up to 6 can archive considerably CLBR as control women. While for the POSEIDON group 4, a simple repeat of the FET cycle is not recommended after four failed FET cycles, some strategies such as PGT-A may be beneficial.

背景:在 POSEIDON 标准中,第 3 组和第 4 组的预后较差。对于卵巢储备不足的患者,通过连续取卵周期积累胚胎进行多次冻融胚胎移植(FET)已变得越来越普遍。有必要在治疗前告知他们单个或多个 FET 周期后的妊娠结果。然而,有关低预后患者累积活产率(CLBR)的研究报道却很少:这项回顾性研究纳入了 2015 年 7 月至 2020 年 8 月期间接受冷冻胚胎移植周期的 4712 名患者。患者被分为POSEIDON第3组、第4组、对照1组(结果:POSEIDON第3组、第4组、对照1组):在乐观模型分析下,对照1组在6个FET周期内的CLBR最高(93.98%,95%CI 91.63-95.67%),其次是POSEIDON 3组女性的CLBR(92.51%,95%CI 77.1-97.55)略低于对照1组。POSEIDON 4 组的 CLBR 最低(55%,95%CI 39.34-70.66%),明显低于对照 2 组(88.7%,95%CI 80.68-96.72%)。此外,POSEIDON 4 组患者在 5 个 FET 周期后达到 CLBR 高点:结论:在临床实践中,POSEIDON 3 组患者可能不被视为传统意义上的 "低预后 "患者,因为与对照组妇女一样,将 FET 周期延长至 6 个周期可显著提高 CLBR。对于POSEIDON 4组患者,在4个FET周期失败后,不建议简单地重复FET周期,一些策略(如PGT-A)可能是有益的。
{"title":"The role of age and AMH on cumulative live birth rates over multiple frozen-thawed embryo transfer cycles: a study based on low prognosis patients of POSEIDON 3 and 4 groups.","authors":"Lan Xia, Xiaowei Zhou, Xiaoling Wang, Shen Zhao, Xian Wu, Huihui Xu, Aijun Zhang, Zhihong Niu","doi":"10.1186/s12958-024-01243-5","DOIUrl":"10.1186/s12958-024-01243-5","url":null,"abstract":"<p><strong>Background: </strong>Among the POSEIDON criteria, group 3 and group 4 have an expected low prognosis. For those patients with inadequate ovary reserve, embryo accumulated from consecutive oocyte retrieval cycles for multiple frozen-thawed embryo transfers (FET) has become more common. It is necessary to inform them of the pregnancy outcomes after single or multiple FET cycles before the treatment. However few studies about cumulative live birth rate (CLBR) for those with low prognosis have been reported.</p><p><strong>Methods: </strong>This retrospective study included 4712 patients undergoing frozen embryo transfer cycles from July 2015 to August 2020. Patients were stratified as POSEIDON group 3, group 4, control 1 group (< 35 years) and control 2 group (≥ 35 years). The primary outcome is CLBRs up to six FET cycles and the secondary outcomes were LBRs per transfer cycle. Optimistic approach was used for the analysis of CLBRs and the depiction of cumulative incidence curves.</p><p><strong>Results: </strong>Under optimistic model analyses, control 1 group exhibited the highest CLBR (93.98%, 95%CI 91.63-95.67%) within 6 FET cycles, followed by the CLBR from women in POSEIDON group 3(92.51%, 95%CI 77.1-97.55)was slightly lower than that in control 1 group. The CLBR of POSEIDON group 4(55% ,95%CI 39.34-70.66%)was the lowest and significantly lower than that of control 2 group(88.7%, 95%CI 80.68-96.72%). Further, patients in POSEIDON group 4 reached a CLBR plateau after 5 FET cycles.</p><p><strong>Conclusions: </strong>The patients of POSEIDON group 3 may not be considered as traditional \"low prognosis\" in clinical practice as extending the number of FET cycles up to 6 can archive considerably CLBR as control women. While for the POSEIDON group 4, a simple repeat of the FET cycle is not recommended after four failed FET cycles, some strategies such as PGT-A may be beneficial.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"69"},"PeriodicalIF":4.4,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11181651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Survival in pregnancy-associated breast cancer patients compared to non-pregnant controls. 更正:与非妊娠对照组相比,妊娠相关乳腺癌患者的生存率。
IF 4.4 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-06-15 DOI: 10.1186/s12958-024-01244-4
María Martín Cameán, Ibon Jaunarena, Jose Ignacio Sánchez-Méndez, Covadonga Martí, Félix Boria, Elena Martín, Emanuela Spagnolo, Ignacio Zapardiel, Alicia Hernández Gutiérrez
{"title":"Correction: Survival in pregnancy-associated breast cancer patients compared to non-pregnant controls.","authors":"María Martín Cameán, Ibon Jaunarena, Jose Ignacio Sánchez-Méndez, Covadonga Martí, Félix Boria, Elena Martín, Emanuela Spagnolo, Ignacio Zapardiel, Alicia Hernández Gutiérrez","doi":"10.1186/s12958-024-01244-4","DOIUrl":"10.1186/s12958-024-01244-4","url":null,"abstract":"","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"68"},"PeriodicalIF":4.4,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11179194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Reproductive Biology and Endocrinology
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