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Biometric Characterization and Morphophysiological Quality of Peach Rootstock Seeds Using Images of their Seedling Vigor. 利用幼苗活力图像对桃源种子进行生物特征鉴定及形态生理品质研究。
Q1 Agricultural and Biological Sciences Pub Date : 2018-01-01 DOI: 10.2174/2212798410666180426155013
Aline G Souza, Oscar J Smiderle, Valmor J Bianchi

Background: This study aimed to evaluate the efficiency of using the computerized imaging Seed Analysis System (SAS) in the biometric and morphophysiological characterization of seeds and the initial growth of seedlings from peach rootstocks.

Methods: The experimental design was completely randomized with five replicates of 20 seeds. The variables analyzed were the seed humidity content, length and width of seeds measured by SAS technology and manual measurements, mean germination time, germination percentage, radicle length and width, taproot length, length of the aerial part and taproot/aerial part ratio.

Results: The highest seed length, germination percentage (100%) and lower germination time (11.3), were obtained with the cv. Capdeboscq while, 'Tsukuba 1', 2' and 3' had intermediate seedlings length, varying from 1.55 to 1.65 cm with mean germination times between 14.5 and 18.0 days and average germination percentage of 96%. The computerized analysis of images is fast and efficient for biometric evaluations such as seed width and length, as well as initial growth of peach tree seedlings. The cvs Capdeboscq, Flordaguard and Tsukuba 2 presented greater radicle width, length and a mean taproot/aerial part ratio equal to 2, as well as higher number of adventitious roots, which indicated a strong positive correlation between radicle length, taproot length and initial seedling growth.

Conclusion: The continuity of the research will certainly allow the development of reliable procedures for other species, besides allowing the identification of wider alternatives for the use of this system for the expansion of knowledge in the areas of physiology and evaluation of the physiological potential of seeds.

背景:本研究旨在评价利用计算机成像种子分析系统(SAS)对桃源砧木种子的生物特征和形态生理特征及幼苗生长初期的有效性。方法:试验设计为完全随机设计,5个重复,每重复20粒种子。分析的变量包括种子湿度含量、SAS技术测定的种子长度和宽度、人工测定的种子长度和宽度、平均发芽时间、发芽率、胚根长度和宽度、主根长度、地上部分长度和主根/地上部分比。结果:不同处理的种子长最高,发芽率最高(100%),萌发时间最低(11.3)。而‘筑波1号’、‘筑波2号’和‘筑波3号’的幼苗长度在1.55 ~ 1.65 cm之间,平均发芽期在14.5 ~ 18.0 d之间,平均发芽率为96%。通过对图像的计算机化分析,可以快速有效地对桃树种子的宽度、长度以及幼苗的初始生长进行生物特征评估。Capdeboscq、Flordaguard和筑波2号的根宽、根长较大,主根/地上部平均比值为2,不定根数量较多,表明根长、主根长与初苗生长有较强的正相关关系。结论:这项研究的连续性肯定会为其他物种开发可靠的程序,除了允许确定更广泛的替代方案,使用该系统来扩展生理学领域的知识和评估种子的生理潜力。
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引用次数: 2
Evaluation of Antioxidant Capacity and Phenolic Content in Ethanolic Extracts of Leaves and Flowers of Some Asteraceae Species. 部分菊科植物叶和花乙醇提取物抗氧化能力及酚类物质含量的评价
Q1 Agricultural and Biological Sciences Pub Date : 2018-01-01 DOI: 10.2174/2212798409666171023150601
Donia Anvari, Rashid Jamei

Background: Medicinal plants contain large amount of natural antioxidant compounds, which enable them to neutralize free radicals. Asteraceae family is an important source of phytochemical compounds.

Objective: The objective of this research is to find out the antioxidant potential and radical scavenging from the leaf and flowers of five species (Artemisia absinthium L., Arctium lappa L., Silybum marianum L., Centaurea cyanus L. and Echinops ritro L.) of the Asteraceae family. This research includes recent patents (US20110179530A1) in plant biotechnology.

Methods: The plant extract obtained by absolute extraction method was investigated for Total Phenolic Content (TPC), total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC), and Chain Breaking Activity (CBA). THIOBARBIOTURIC ACID (TBA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), IC50, nitric oxide (NO), hydrogen peroxide (H2O2), superoxide (O-) radical scavenging and Ferric Reducing Antioxidant Power (FRAP) were also measured.

Results: The results showed the highest values for the TPC, TBA, FRAP and DPPH radical scavenging related to A. absinthium. While, A. lappa extract showed the maximum content for TFC, TAC, CBA and O2 radical scavenging. In addition, our results indicated that E. ritro is a good source of NO2 and H2O2 radical scavenging activity (P<0.05). Our findings suggest that the flower is more successful than the leaf.

Conclusion: Artemisia absinthium, A. lappa and E. ritro can be used as an easily accessible source of natural antioxidants and as a possible food supplement or in pharmaceutical industry.

背景:药用植物含有大量的天然抗氧化化合物,使其能够中和自由基。菊科是植物化学物质的重要来源。目的:研究菊科5种植物(苦艾、牛蒡、水飞蓟、Centaurea cyanus L.和Echinops ritro L.)的叶和花的抗氧化能力和自由基清除能力。本研究包括植物生物技术最新专利(US20110179530A1)。方法:采用绝对提取法对提取的植物提取物进行总酚含量(TPC)、总黄酮含量(TFC)、总抗氧化能力(TAC)和断链活性(CBA)的测定。测定了硫代巴比图酸(TBA)、2,2-二苯基-1-苦味酰肼(DPPH)、IC50、一氧化氮(NO)、过氧化氢(H2O2)、超氧化物(O-)自由基清除能力和铁还原抗氧化能力(FRAP)。结果:苦艾草对TPC、TBA、FRAP和DPPH自由基的清除能力最高。枸杞提取物对TFC、TAC、CBA和O2自由基的清除作用最大。结论:苦艾草、黄花蒿和黄花蒿均可作为一种易于获取的天然抗氧化剂来源,并可作为食品补充剂或制药工业的潜在原料。
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引用次数: 7
Flow Cytometric Analysis of Pro-inflammatory Cytokine Production in Hyperglycemic Mouse Model. 高血糖小鼠模型中促炎细胞因子产生的流式细胞术分析。
Q1 Agricultural and Biological Sciences Pub Date : 2018-01-01 DOI: 10.2174/2212798410666180426161016
Muhaimin Rifa I, Mulya D Wahyuningsih, Noviana D Lestari, Aris Soewondo, Mansur Ibrahim, Hideo Tsuboi

Objective: The sharp increase of pro-inflammatory cytokine becomes a severe problem in some degenerative diseases. This study aimed to investigate the effect of Cherry Leaf Extract (CLE) on pro-inflammatory cytokine production in hyperglycaemic mouse model. A recent patent mentioned that Muntinga calabura is a flavonoid-containing plant which is capable of reinforcing the stomach, appetizing, helping digestion (CN 201611138374), and improving stomach distention (CN106071017A). The efficacy of this miracle plant has inspired this experiment.

Methods: In this study, we induced hyperglycemia with high-fat diet and 20% fructose drink. Hyperglycemic mice were orally administered with CLE with the doses 420, 700, and 28000 mg/kg BW for two weeks. After treatment, the pro-inflammatory molecule was analyzed by flow cytometry from splenic cells.

Results: This study showed that CLE decreased the production of pro-inflammatory molecules such as IL-6, NF-κB, TNF-α, and IFN γ. In hyperglycemic mice, we found CD68+IL-6+, NK1.1+IL- 6+, B220+NFκB+, CD4+TNF-α+, and CD4+IFN γ + increased significantly (p < 0.05) compared to control mice without manipulation. After treatment with 420, 700, or 2800 mg/kg BW of CLE, the pro-inflammatory molecules decreased significantly (p < 0.05), and reached a normal physiology level.

Conclusion: CLE can suppress pro-inflammatory cytokines in hyperglycemic mouse model, suggesting this medicinal herb may be beneficial as a potential strategy for future therapeutic interventions in degenerative diseases or a disease involving cell activation.

目的:促炎细胞因子的急剧升高已成为一些退行性疾病的严重问题。本研究旨在探讨樱桃叶提取物(CLE)对高血糖小鼠模型中促炎细胞因子产生的影响。最近的一项专利提到,芒tinga calabura是一种含类黄酮的植物,具有补胃、开胃、助消化(CN 201611138374)和胃胀(CN 106071017a)的作用。这种神奇植物的功效激发了这个实验的灵感。方法:采用高脂饮食和20%果糖饮料诱导高血糖。给高血糖小鼠以420、700和28000 mg/kg BW剂量口服CLE,持续两周。治疗后,用流式细胞术分析脾细胞的促炎分子。结果:本研究显示,CLE可降低IL-6、NF-κB、TNF-α、IFN γ等促炎分子的产生。在高血糖小鼠中,我们发现CD68+IL-6+、NK1.1+IL- 6+、B220+NFκB+、CD4+TNF-α+、CD4+IFN γ +与未加操作的对照组相比显著升高(p < 0.05)。给药420、700、2800 mg/kg BW后,促炎分子水平显著降低(p < 0.05),达到正常生理水平。结论:CLE可抑制高血糖小鼠模型中的促炎细胞因子,提示该草药可能是未来退行性疾病或涉及细胞活化的疾病的潜在治疗干预策略。
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引用次数: 1
Orientation of Agri-Food Companies to CSR and Consumer Perception: A Survey on Two Italian Companies. 农业食品企业社会责任取向与消费者认知:对两家意大利企业的调查。
Q1 Agricultural and Biological Sciences Pub Date : 2018-01-01 DOI: 10.2174/2212798410666180508103619
Gennaro Civero, Vincenzo Rusciano, Debora Scarpato

Background: Corporate Social Responsibility (CSR) is the most important tool for implementing Sustainability Guidelines for Business (US20030018487A1), delivering economic, social and environmental benefits for all the stakeholders and is currently the focus of international studies and debates (US7260559B1), especially in the agri-food sector as demonstrated by recent patents (CA2862273A1). In most agri-food businesses operating in Italy, there is little effectiveness in the communication strategies of this instrument to the stakeholders since they are often not advanced.

Methods: Identifying the first two food companies in the European ranking of the 6th CSR Online Awards, through an empirical survey on consumers, their level of perception of the CSR strategies communication of these companies will be analyzed.

Results: In both case studies analyzed, there is the presence of a Sustainability Orientation and the evolution of CSR tools within such companies and their promotion and communication to all the stakeholders. Despite this, the level of perception of respondents on the CSR strategies communication of these companies and the promotion of CSR instruments is not optimal.

Conclusion: The interviewed consumers have not enriched their knowledge on CSR due to the lack of an adequate communication strategy for both case studies. Consequently, to raise awareness on the subject, there is still a great deal of cooperation needed between public institutions, local communities, businesses and citizens.

背景:企业社会责任(CSR)是实施《企业可持续发展指南》(US20030018487A1)最重要的工具,为所有利益相关者提供经济、社会和环境效益,目前是国际研究和辩论的焦点(US7260559B1),特别是在农业食品领域,最近的专利(CA2862273A1)证明了这一点。在意大利经营的大多数农业食品企业中,这一工具与利益相关者的沟通策略几乎没有效果,因为它们往往不先进。方法:选取第六届企业社会责任网络大奖欧洲排名前两名的食品企业,通过对消费者的实证调查,分析消费者对这些企业企业社会责任战略传播的感知程度。结果:在分析的两个案例研究中,这些公司都存在可持续发展取向和企业社会责任工具的演变,以及它们对所有利益相关者的推广和沟通。尽管如此,受访者对这些公司的企业社会责任战略沟通和企业社会责任工具推广的认知水平并不理想。结论:由于缺乏适当的沟通策略,受访的消费者并没有丰富他们对企业社会责任的知识。因此,为了提高对这一问题的认识,公共机构、地方社区、企业和公民之间仍然需要进行大量合作。
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引用次数: 8
Edible Mushrooms from Niger Delta, Nigeria with Heavy Metal Levels of Public Health Concern: A Human Health Risk Assessment. 来自尼日利亚尼日尔三角洲的食用菌重金属含量对公众健康的影响:人体健康风险评估。
Q1 Agricultural and Biological Sciences Pub Date : 2018-01-01 DOI: 10.2174/2212798409666171129173802
Sorbari Igbiri, Nnaemeka A Udowelle, Osazuwa C Ekhator, Rose N Asomugha, Zelinjo N Igweze, Orish E Orisakwe

Background: Mushrooms (US6759049B2, CA2372185A1, DE60108325D1) can be contaminated by heavy metals as described in various patents.

Objective: This study has determined the potential human health risk associated with the consumption of mushroom in Niger Delta, Nigeria. Concentrations of Pb, Cd, Sn, Cu, Ni, Al and Zn in both wild/cultivated mushroom and background soil/substrate were determined using Atomic Absorption Spectrometer (AAS).

Methods: Heavy metal pollution was calculated using Bio-Concentration Factor (BCF) and Pollution Load Index (PLI) while human health risk assessment employed Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), Hazard Index (HI), Carcinogenic Risk (CR) and Total Cancer Risk (TCR). The mean concentration of metals in mushroom and soil/substrate ranged from 0.021 mg/kg - 29.16 mg/kg and 0.01 mg/kg - 56.11 mg/kg. When compared with standards by WHO, EU, SON, DPR, USEPA and NAFDAC, 100% violation was observed in Ni, Cu, Cd, Zn and partial violation by Pb in the mushroom sample although soil heavy metal concentrations were within safe limits.

Results: There was bioconcentration and heavy metal enrichment as PLI ranged from 3.39 - 23.58. Estimated daily intakes were (0.00 - 0.47, 0.00 - 0.51 and 0.00 - 1.17) mg/kg/day and THQ were (0.01 - 0.46, 0.01 - 0.31 and 0.03 - 1.51) for adults, adolescents and children respectively. There was a violation of Permissible Tolerable Daily Intakes (PTDI) set by JECFA. Hazard indices were all >1, another indication of health concern.

Conclusion: Nickel showed the highest carcinogenic risk with values of 4.83E-04 - 1.43E-01, 5.31E-04 - 1.00E-01 and 1.20E-03 - 2.29E-01 for adults, adolescents and children respectively. About 80% of TCR ranged from x10-1 - x10-3. Consumption of mushroom in Niger delta-Nigeria may pose significant health risk.

背景:蘑菇(US6759049B2, CA2372185A1, DE60108325D1)可被各种专利描述的重金属污染。目的:本研究确定了尼日利亚尼日尔三角洲地区蘑菇消费的潜在人类健康风险。采用原子吸收光谱仪(AAS)测定了野生/栽培蘑菇和背景土壤/基质中Pb、Cd、Sn、Cu、Ni、Al和Zn的含量。方法:采用生物浓度因子(BCF)和污染负荷指数(PLI)计算重金属污染,采用估计每日摄入量(EDI)、目标危害商(THQ)、危害指数(HI)、致癌风险(CR)和总致癌风险(TCR)进行人体健康风险评价。蘑菇和土壤/基质中金属的平均浓度为0.021 mg/kg ~ 29.16 mg/kg和0.01 mg/kg ~ 56.11 mg/kg。与WHO、EU、SON、DPR、USEPA、NAFDAC等标准比较,蘑菇样品中Ni、Cu、Cd、Zn均100%超标,Pb部分超标,土壤重金属含量均在安全范围内。结果:生物富集和重金属富集,PLI范围为3.39 ~ 23.58。成人、青少年和儿童的估计日摄入量分别为(0.00 - 0.47、0.00 - 0.51和0.00 - 1.17)mg/kg/d, THQ分别为(0.01 - 0.46、0.01 - 0.31和0.03 - 1.51)。违反了JECFA规定的每日容许容许摄入量(PTDI)。危害指数均>1,再次提示存在健康问题。结论:镍对成人、青少年和儿童的致癌风险最高,分别为4.83E-04 ~ 1.43E-01、5.31E-04 ~ 1.00E-01和1.20E-03 ~ 2.29E-01。约80%的TCR范围为x10-1 - x10-3。在尼日尔三角洲-尼日利亚食用香菇可能会造成重大的健康风险。
{"title":"Edible Mushrooms from Niger Delta, Nigeria with Heavy Metal Levels of Public Health Concern: A Human Health Risk Assessment.","authors":"Sorbari Igbiri,&nbsp;Nnaemeka A Udowelle,&nbsp;Osazuwa C Ekhator,&nbsp;Rose N Asomugha,&nbsp;Zelinjo N Igweze,&nbsp;Orish E Orisakwe","doi":"10.2174/2212798409666171129173802","DOIUrl":"https://doi.org/10.2174/2212798409666171129173802","url":null,"abstract":"<p><strong>Background: </strong>Mushrooms (US6759049B2, CA2372185A1, DE60108325D1) can be contaminated by heavy metals as described in various patents.</p><p><strong>Objective: </strong>This study has determined the potential human health risk associated with the consumption of mushroom in Niger Delta, Nigeria. Concentrations of Pb, Cd, Sn, Cu, Ni, Al and Zn in both wild/cultivated mushroom and background soil/substrate were determined using Atomic Absorption Spectrometer (AAS).</p><p><strong>Methods: </strong>Heavy metal pollution was calculated using Bio-Concentration Factor (BCF) and Pollution Load Index (PLI) while human health risk assessment employed Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), Hazard Index (HI), Carcinogenic Risk (CR) and Total Cancer Risk (TCR). The mean concentration of metals in mushroom and soil/substrate ranged from 0.021 mg/kg - 29.16 mg/kg and 0.01 mg/kg - 56.11 mg/kg. When compared with standards by WHO, EU, SON, DPR, USEPA and NAFDAC, 100% violation was observed in Ni, Cu, Cd, Zn and partial violation by Pb in the mushroom sample although soil heavy metal concentrations were within safe limits.</p><p><strong>Results: </strong>There was bioconcentration and heavy metal enrichment as PLI ranged from 3.39 - 23.58. Estimated daily intakes were (0.00 - 0.47, 0.00 - 0.51 and 0.00 - 1.17) mg/kg/day and THQ were (0.01 - 0.46, 0.01 - 0.31 and 0.03 - 1.51) for adults, adolescents and children respectively. There was a violation of Permissible Tolerable Daily Intakes (PTDI) set by JECFA. Hazard indices were all >1, another indication of health concern.</p><p><strong>Conclusion: </strong>Nickel showed the highest carcinogenic risk with values of 4.83E-04 - 1.43E-01, 5.31E-04 - 1.00E-01 and 1.20E-03 - 2.29E-01 for adults, adolescents and children respectively. About 80% of TCR ranged from x10-1 - x10-3. Consumption of mushroom in Niger delta-Nigeria may pose significant health risk.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"9 1","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798409666171129173802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35648885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Occurrence and Importance of Plant Lipids: A Promising Insight into Algae. 植物脂质的发生和重要性:对藻类的展望。
Q1 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.2174/2212798409666170619101051
Nihed B Halima

Background: Lipids are biomolecules with interesting structural variability. They are involved in many processes such as the storage of energy, in nutrition and are also of relevance for signal transduction processes, in apoptosis, cell differentiation and phagocytosis, etc. Macroalgae and microalgae are a promising and very diverse group of organisms. These living arganisms inhabit a vaste range of ecosystems from the Antarctic to the Deserts. They account for above half the primary productivity at the base of the food chain because of their multiple nutritional products including, for instance, lipids.

Results: Recently, studies on the oleaginous algae encompass their uses for commercial exploitations with applications ranging from human health food, animal feed, aquaculture, nutraceutical, biofuels and others. In this article, updated knowledge of lipids and recent research studies for algae's valorization performed by several authors were reviewed. Special attention was paid to lipids accumulation and their characterization.

Conclusion: The selection of the prominent species of algae will be of great importance to satisfy the corresponding valorization process. Patents identified with algal lipids composition, production and application are presented.

背景:脂质是具有有趣的结构变异性的生物分子。它们参与许多过程,如能量储存、营养,也与信号转导过程、细胞凋亡、细胞分化和吞噬等有关。巨藻和微藻是一种极具发展前景且种类繁多的生物。这些生物生活在从南极到沙漠的广阔生态系统中。它们占了食物链底部初级生产力的一半以上,因为它们有多种营养产品,例如脂类。结果:最近,对产油藻类的研究包括其商业开发用途,应用范围包括人类保健食品、动物饲料、水产养殖、营养食品、生物燃料等。本文综述了脂类的最新知识和最近几位作者对藻类增殖的研究进展。特别注意脂质积累及其表征。结论:选择突出的藻类品种对满足相应的增殖过程具有重要意义。介绍了藻脂的组成、生产和应用方面的专利认定。
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引用次数: 1
The Role of Saffron in Attenuating Age-related Oxidative Damage in Rat Hippocampus. 藏红花在大鼠海马衰老性氧化损伤中的作用。
Q1 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.2174/2212798409666170529073601
Saeed Samarghandian, Mohsen Azimi-Nezhad, Fariborz Samini, Tahereh Farkhondeh

Background: Using antioxidant diets may be a good strategy for the inhibition of oxidative stress connected to age-related disease. Saffron is a dried stigma of with antioxidant properties. This investigation was done to study the effects of saffron administration on lipid peroxidation and antioxidant status in female aged rats.

Methods: The rats were treated with the ethanolic extract of saffron for 4 weeks. At the end of our investigation, the hippocampi of rats were removed. The homogenate was used for the evaluation of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxides (GPx), catalase (CAT) and glutathione (GSH). There are recent patents also suggesting the use of saffron and its gradients for prevention of oxidative stress in aged tissues.

Result: The results showed that the aging was connected with a significant reduction in the activities of antioxidant enzymes, and an increase in MDA and nitric oxide levels in the hippocampus of aged rats. In addition, treatment of saffron was effective in reducing hippocampus MDA and NO levels and in increasing the antioxidant status. There is a possibility that saffron extract acts as a hormetin by inducing mild oxidative damage which leads to the activation of antioxidative enzymes.

Conclusion: Therefore, saffron exerted considerable neuroprotective effect and proved to be efficacious in protecting rat hippocampus against age-related oxidative damage.

背景:使用抗氧化饮食可能是抑制与年龄相关疾病相关的氧化应激的良好策略。藏红花是一种具有抗氧化特性的干柱头。本实验研究了藏红花对雌性老龄大鼠脂质过氧化和抗氧化能力的影响。方法:大鼠用藏红花乙醇提取物灌胃4周。在研究结束时,我们切除了大鼠的海马体。用匀浆评价丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物(GPx)、过氧化氢酶(CAT)和谷胱甘肽(GSH)的含量。最近也有专利建议使用藏红花及其梯度预防老化组织中的氧化应激。结果:老龄大鼠海马抗氧化酶活性显著降低,MDA和一氧化氮水平升高,与衰老有关。此外,藏红花处理有效降低海马MDA和NO水平,提高抗氧化能力。有一种可能性是,藏红花提取物通过诱导轻度氧化损伤,从而导致抗氧化酶的激活,从而起到促激素的作用。结论:藏红花具有明显的神经保护作用,对大鼠海马抗衰老氧化损伤具有一定的保护作用。
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引用次数: 15
Multiplex-PCR As a Rapid and Sensitive Method for Identification of Meat Species in Halal-Meat Products. 多重pcr作为一种快速、灵敏的清真肉制品中肉类种类鉴定方法。
Q1 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.2174/2212798409666170113151213
Mahsa Alikord, J. Keramat, M. Kadivar, H. Momtaz, M. N. Eshtiaghi, A. Homayouni‐Rad
BACKGROUND Species identification and authentication in meat products are important subjects for ensuring the health of consumers. The multiplex-PCR amplification and species- specific primer set were used for the identification of horse, donkey, pig and other ruminants in raw and processed meat products. METHODS Oligonucleotid primers were designed and patented for amplification of species-specific mitochondrial DNA sequences of each species and samples were prepared from binary meat mixtures. RESULTS The results showed that meat species were accurately determined in all combinations by multiplex-PCR, and the sensitivity of this method was 0.001 ng, rendering this technique open to and suitable for use in industrial meat products. It is concluded that more fraud is seen in lower percentage industrial meat products than in higher percentage ones. There was also more fraud found in processed products than in raw ones. CONCLUSION This rapid and useful test is recommended for quality control firms for applying more rigorous controls over industrial meat products, for the benefit of target consumers.
背景:肉制品中的物种识别和认证是保障消费者健康的重要课题。利用多重pcr扩增和物种特异性引物对马、驴、猪等反刍动物的生肉和加工肉制品进行鉴定。方法设计并申请专利的单核苷酸引物,用于扩增各物种的线粒体DNA序列,并从二元肉混合物中制备样品。结果多重聚合酶链反应(multiple - pcr)可准确测定肉制品种类,方法灵敏度为0.001 ng,可用于肉制品工业生产。结论是,比例较低的工业肉制品比比例较高的工业肉制品更容易出现欺诈行为。在加工产品中发现的欺诈行为也比在生产品中发现的更多。结论该快速、有效的检测方法可用于质量控制公司对工业肉制品进行更严格的控制,有利于目标消费者。
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引用次数: 12
Potential of Macroalgae Ulva lactuca as a Source Feedstock for Biodiesel Production. 大型海藻Ulva lactuca作为生物柴油原料的潜力。
Q1 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.2174/2212798409666170602080725
Hanaa H Abd El Baky, Gamal S El Baroty

Background: The aim of this study was to investigate the possibility of growing of algae Ulva lactuca L.under different salinity levels coupled with varied KNO3 concentrations (source of N) as a potential source of oil for biodiesel production.

Methods: U. lactuta was cultured in 10.0% NaCl coupled with either 2.5 g/L (S1+ 1N) or 1.0 g/L KNO3 (S1+ 2N) and in 30.0% NaCl coupled with 2.5 g/L (S2+ 1N) or 1.0 g/L KNO3 (S2+ 2N) nutrient medium. Among all algae cultures, biomass (dry weight) and lipid accumulation (total lipid content, TL) were significantly different (P>0.5%), with various degrees. The TL was increased (8.21% to 15.95%, g/100g) by increasing the NaCl % (from 10% to 30%) coupled with the depletion of KNO3 level (from 2.5% to 1%) in culture medium. High lipid content (15.95%) was obtained in S2+ 2N culture, this lipid showed physical (density, viscosity and average molecular weight) and chemical (iodine, acid, saponification and peroxide values) properties suitable for biodiesel production.

Results: The fatty acid methyl esters (FAME, biodiesel) prepared by trans-esterifiction reaction under acidic condition were mainly composed of saturated (50.33%), monounsaturated (MUFA, 36.12%) and polyunsaturated (13.55%) esters. C-18:1 was found to be the main MUFA, representing 25.76% of total FAME. On the other hand, the values of some critical of physiochemical parameter (density, kinematic viscosity, iodine value, acid value and oxidation stability) of biodiesel were found to meet the standards for a high quality biodiesel.

Conclusion: Hence, U. lactuta could be serving as a valuable renewable biomass of oil for biodiesel production. There are recent patents also suggesting use of oil of U. lactuta marine biomass for biodiesel production.

背景:本研究的目的是探讨在不同盐度水平和不同KNO3浓度(N源)条件下,海藻Ulva lactuca l.s生长的可能性,作为生产生物柴油的潜在油源。方法:乳乳杆菌在10.0% NaCl + 2.5 g/L (S1+ 1N)或1.0 g/L KNO3 (S1+ 2N)培养基和30.0% NaCl + 2.5 g/L (S2+ 1N)或1.0 g/L KNO3 (S2+ 2N)培养基中培养。在所有培养物中,生物量(干重)和脂质积累(总脂质含量,TL)均有不同程度的显著差异(P>0.5%)。将培养基中NaCl浓度从10%提高到30%,同时降低KNO3浓度(从2.5%降低到1%),可使TL提高8.21% ~ 15.95% (g/100g)。在S2+ 2N培养基中获得了高脂质含量(15.95%),该脂质具有适合生产生物柴油的物理(密度、粘度和平均分子量)和化学(碘、酸、皂化和过氧化值)特性。结果:酸性条件下反式酯化反应制备的脂肪酸甲酯(FAME,生物柴油)主要由饱和(50.33%)、单不饱和(MUFA, 36.12%)和多不饱和(13.55%)酯组成。发现C-18:1是主要的MUFA,占总FAME的25.76%。另一方面,生物柴油的一些关键理化参数(密度、运动粘度、碘值、酸值和氧化稳定性)的值符合高质量生物柴油的标准。结论:乳曲霉可作为一种有价值的可再生生物质油脂用于生物柴油的生产。最近也有一些专利建议使用海洋生物油来生产生物柴油。
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引用次数: 6
New Advances in Infant Feeding: New Products and Novel Technologies. 婴儿喂养的新进展:新产品和新技术。
Q1 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.2174/2212798409666170328145150
Maria C Pina-Pérez, Antonio Martínez, Dolores Rodrigo

Background: Breastfeeding is the best way to provide ideal food for infants. However, there are many situations in which breastfeeding is unviable (maternal illness, hygiene problems, intravenous feeding requirement, storage). Safe, efficient, and nutritious products should be offered by the industry to mothers and healthcare professionals as an alternative.

Objective: The present review aims to update the state of the art regarding the most innovative developments in infant feeding formulation and, the promising novel technologies that are being investigated to achieve a balance between the microbiological stability, organoleptic and nutritional value of baby food.

Methods: The research lines included in the present systematic review are focused on both, the most innovative ingredients and emergent technologies applied increasing the offer of tailor-made nutritional profiled products with improved quality and safety.

Results: According to the reviewed research and recently published patents the current emergence of a new generation of infant products is remarkable, with specific product lines aimed at infants at different stages of development and affected by several disorders (low-weight babies, premature neonates, allergenic patients), with special emphasis on the application of novel technologies (e.g. High Hydrostatic Pressure (HHP) and microencapsulation) as potential techniques to ensure the microbiological safety of developed products, and the improvement of their nutritional value, complementary to the addition of functional ingredients, such as omega 3 and 6 fatty acids, amino acids, prebiotics and probiotics, and medicinal herb supplementation (e.g. lemon balm, royal jelly).

Conclusion: The present research work provides a general view of recent advances in infant products processing and formulation focusing on the technological effects and quality/safety developments.

背景:母乳喂养是为婴儿提供理想食物的最佳方式。然而,在许多情况下,母乳喂养是不可行的(产妇疾病、卫生问题、静脉喂养要求、储存)。该行业应向母亲和保健专业人员提供安全、高效和营养的产品作为替代。目的:本综述旨在更新婴儿喂养配方的最新发展,以及正在研究的有前途的新技术,以实现婴儿食品的微生物稳定性,感官和营养价值之间的平衡。方法:本系统综述的研究重点是最具创新性的成分和新兴技术的应用,增加了量身定制的营养产品的质量和安全性。结果:根据审查的研究和最近公布的专利,目前新一代婴儿产品的出现是显着的,针对不同发育阶段和受几种疾病影响的婴儿(低体重婴儿,早产儿,过敏性患者)的特定产品线。特别强调新技术的应用(如高静水压力(HHP)和微胶囊)作为潜在技术,以确保所开发产品的微生物安全性,并提高其营养价值,补充功能性成分,如omega 3和6脂肪酸,氨基酸,益生元和益生菌,以及草药补充剂(如柠檬香蜂草,蜂王浆)。结论:本研究工作概述了婴儿用品加工和配方的最新进展,重点是技术效果和质量/安全发展。
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Recent patents on food, nutrition & agriculture
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