Alessandra dos Santos Danziger Silvério, R. T. G. Pereira, S. Duarte, S. Figueiredo, F. Paula, Tomaz Henrique Araújo, C. Ferreira, B. C. C. Salles, A. L. M. Viana, E. Ferreira, M. R. Rodrigues
Objectives: The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiments were approved by the ethics committee for animal experiments under N°. 420/2012 and 536/2013. Diabetic and non-diabetic rats were divided into 6 groups treated and untreated with coffee (7.2 mL/Kg body weight) and aminoguanidine (AGE inhibiting agent) (100 mg/Kg body weight) for 50 days. After 50 days, the animals were fasted for 12 h and anesthetized (40 mg/Kg sodium pentobarbital) intraperitoneally. Blood samples were collected from the abdominal artery puncture. Hematological parameters (red cells, hemoglobin, hematocrit and leukocyte) and glycemic and HbA1c levels were measured. AGEs quantification (spectrofluorometric method) and the platelet aggregation test (aggregation of cuvettes in a four-channel platelet aggregometer) were also conducted. The rats’ renal function was evaluated by measuring serum urea and creatinine. Results: Data showed that coffee intake had no effect on the hematological parameters. Fasting glucose and HbA1c dosage were significantly higher in diabetic animals compared to non-diabetic animals (confirmed the effectiveness of inducing and maintaining diabetic status). Results showed that coffee reduced AGE formation and platelet aggregation in our animal model, not altering the animals’ renal function. Conclusions: These results suggest beneficial effects on vasculopathy, a common complication in diabetic patients.
{"title":"Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats","authors":"Alessandra dos Santos Danziger Silvério, R. T. G. Pereira, S. Duarte, S. Figueiredo, F. Paula, Tomaz Henrique Araújo, C. Ferreira, B. C. C. Salles, A. L. M. Viana, E. Ferreira, M. R. Rodrigues","doi":"10.4322/2179-443X.0711","DOIUrl":"https://doi.org/10.4322/2179-443X.0711","url":null,"abstract":"Objectives: The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiments were approved by the ethics committee for animal experiments under N°. 420/2012 and 536/2013. Diabetic and non-diabetic rats were divided into 6 groups treated and untreated with coffee (7.2 mL/Kg body weight) and aminoguanidine (AGE inhibiting agent) (100 mg/Kg body weight) for 50 days. After 50 days, the animals were fasted for 12 h and anesthetized (40 mg/Kg sodium pentobarbital) intraperitoneally. Blood samples were collected from the abdominal artery puncture. Hematological parameters (red cells, hemoglobin, hematocrit and leukocyte) and glycemic and HbA1c levels were measured. AGEs quantification (spectrofluorometric method) and the platelet aggregation test (aggregation of cuvettes in a four-channel platelet aggregometer) were also conducted. The rats’ renal function was evaluated by measuring serum urea and creatinine. Results: Data showed that coffee intake had no effect on the hematological parameters. Fasting glucose and HbA1c dosage were significantly higher in diabetic animals compared to non-diabetic animals (confirmed the effectiveness of inducing and maintaining diabetic status). Results showed that coffee reduced AGE formation and platelet aggregation in our animal model, not altering the animals’ renal function. Conclusions: These results suggest beneficial effects on vasculopathy, a common complication in diabetic patients.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"455 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79759632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luiz Fernando Rodrigues Ferreira, Douglas Vieira Thomaz, M. P. F. Duarte, R. Lopez, V. Pedrazzi, O. Freitas, R. O. Couto
Objectives: To systematically evaluate the effects of hydroxypropyl methyl cellulose (HPMC) type (E5LV, E15LV, and K100LV); plasticizer type (glycerol and mannitol), plasticizer loading (0.12 and 0.24% w/w); and loading of prilocaine and lidocaine hydrochlorides combined at 1:1 ratio (0 and 47 mg/cm2) in the mechanical properties of buccal films. Methods: A quality by design (QbD) approach based on a full factorial design (3 x 23) and complementarily multivariate statistical tools i.e., principal component analysis (PCA), response surface methodology (RSM), and correlation matrix were used in this pursuit. The thickness, elongation at break, tensile strength, force at break, and Young`s modulus of the anesthetic buccal films obtained by solvent casting were assessed. Results: The QbD, PCA and RSM altogether demonstrated that all studied formulation variables, mainly the drug loading, affect the mechanical properties of the films at different significance levels. The multivariate analysis yielded the modelling of elongation at break, tensile strength, and force at break, which significantly correlated with each other. The drugs exerted a synergic plasticizing effect on the films, and the use of HPMC K100 LV (with greater hydroxypropyl substitution degree and viscosity) and mannitol favored their elasticity and resistance. Furthermore, the majority of the films fulfilled the requirements for buccal administration due to their softness and mechanical resistance. Conclusion: Mannitol is suitable plasticizer for manufacturing HPMC anesthetic buccal films with improved mechanical properties. These results are a step forward in the rational development of formulations for the replacement of needles in dentistry
{"title":"Quality by Design-driven investigation of the mechanical properties of mucoadhesive films for needleless anesthetics administration","authors":"Luiz Fernando Rodrigues Ferreira, Douglas Vieira Thomaz, M. P. F. Duarte, R. Lopez, V. Pedrazzi, O. Freitas, R. O. Couto","doi":"10.4322/2179-443X.0707","DOIUrl":"https://doi.org/10.4322/2179-443X.0707","url":null,"abstract":"Objectives: To systematically evaluate the effects of hydroxypropyl methyl cellulose (HPMC) type (E5LV, E15LV, and K100LV); plasticizer type (glycerol and mannitol), plasticizer loading (0.12 and 0.24% w/w); and loading of prilocaine and lidocaine hydrochlorides combined at 1:1 ratio (0 and 47 mg/cm2) in the mechanical properties of buccal films. Methods: A quality by design (QbD) approach based on a full factorial design (3 x 23) and complementarily multivariate statistical tools i.e., principal component analysis (PCA), response surface methodology (RSM), and correlation matrix were used in this pursuit. The thickness, elongation at break, tensile strength, force at break, and Young`s modulus of the anesthetic buccal films obtained by solvent casting were assessed. Results: The QbD, PCA and RSM altogether demonstrated that all studied formulation variables, mainly the drug loading, affect the mechanical properties of the films at different significance levels. The multivariate analysis yielded the modelling of elongation at break, tensile strength, and force at break, which significantly correlated with each other. The drugs exerted a synergic plasticizing effect on the films, and the use of HPMC K100 LV (with greater hydroxypropyl substitution degree and viscosity) and mannitol favored their elasticity and resistance. Furthermore, the majority of the films fulfilled the requirements for buccal administration due to their softness and mechanical resistance. Conclusion: Mannitol is suitable plasticizer for manufacturing HPMC anesthetic buccal films with improved mechanical properties. These results are a step forward in the rational development of formulations for the replacement of needles in dentistry","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88800723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. M. Feitosa, Thamires Sousa de Alencar Silva, Ana Elizabete Xavier Fonseca, Wendel Clei Souza Rodrigues, Amanda Carolline Esquerdo da Silva, Cássia Valéria Pinheiro Corrêa, F. S. Aguiar, R. Mourão, Edilene Gadelha de Oliveira, Kariane M. Nunes
A new consumer profile for pharmaceutical and cosmetic products has motivated research into natural raw materials in the development of “green” products such as herbal medicines and biocosmetics. However, various limitations have been encountered in the marketing of these products, for example the quality control of the natural raw materials used by the industrial market. This study aims to evaluate the sensory and physicochemical parameters of murumuru (Astrocaryum murumuru Mart.), bacuri (Platonia insignis Mart.), tucuma (Astrocaryum vulgare Mart.), and ucuuba (Virola sebifera Aubl.) butters for applications in pharmaceutical and cosmetic bioproducts. The acidity and saponification as well as the iodine and peroxide indexes were evaluated and fatty acid profiles for the samples obtained by GC-MS. The sensory properties of the butters showed the appearance of solid to soft cream, color (yellow, brown, buttercup, and ochre), and characteristic odor. The melting temperatures of all butters ranged between 31 oC and 49 °C. The acidity, saponification, iodine and peroxide indexes for the butters were of 5.82 – 17.73 mg (NaOH or KOH) g−1, 181.10 – 573.55 mg KOH g−1, 2.78 – 44.96 gl2 100 g−1, and 1.39 – 9.30 meq kg−1, respectively. From analyses of the fatty acid profiles, the major components identified were lauric acid in murumuru (40%) and ucuuba butters (73%), myristic acid in tucuma butter (53%), and palmitic acid in bacuri butter (42%). In general, the results of the analyses differed from the specifications of the supplier reports and official compendia. These findings highlight the importance of quality control in natural raw materials to ensure their functionality in pharmaceutical and cosmetic bioproducts.
{"title":"Evaluation of the quality of Amazonian butters as sustainable raw materials for applications in bioproducts","authors":"J. M. Feitosa, Thamires Sousa de Alencar Silva, Ana Elizabete Xavier Fonseca, Wendel Clei Souza Rodrigues, Amanda Carolline Esquerdo da Silva, Cássia Valéria Pinheiro Corrêa, F. S. Aguiar, R. Mourão, Edilene Gadelha de Oliveira, Kariane M. Nunes","doi":"10.4322/2179-443X.0708","DOIUrl":"https://doi.org/10.4322/2179-443X.0708","url":null,"abstract":"A new consumer profile for pharmaceutical and cosmetic products has motivated research into natural raw materials in the development of “green” products such as herbal medicines and biocosmetics. However, various limitations have been encountered in the marketing of these products, for example the quality control of the natural raw materials used by the industrial market. This study aims to evaluate the sensory and physicochemical parameters of murumuru (Astrocaryum murumuru Mart.), bacuri (Platonia insignis Mart.), tucuma (Astrocaryum vulgare Mart.), and ucuuba (Virola sebifera Aubl.) butters for applications in pharmaceutical and cosmetic bioproducts. The acidity and saponification as well as the iodine and peroxide indexes were evaluated and fatty acid profiles for the samples obtained by GC-MS. The sensory properties of the butters showed the appearance of solid to soft cream, color (yellow, brown, buttercup, and ochre), and characteristic odor. The melting temperatures of all butters ranged between 31 oC and 49 °C. The acidity, saponification, iodine and peroxide indexes for the butters were of 5.82 – 17.73 mg (NaOH or KOH) g−1, 181.10 – 573.55 mg KOH g−1, 2.78 – 44.96 gl2 100 g−1, and 1.39 – 9.30 meq kg−1, respectively. From analyses of the fatty acid profiles, the major components identified were lauric acid in murumuru (40%) and ucuuba butters (73%), myristic acid in tucuma butter (53%), and palmitic acid in bacuri butter (42%). In general, the results of the analyses differed from the specifications of the supplier reports and official compendia. These findings highlight the importance of quality control in natural raw materials to ensure their functionality in pharmaceutical and cosmetic bioproducts.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"47 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73419243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sousa, Maria Denise Leite Ferreira, D. Fernandes, L. Cordeiro, M. F. Souza, H. Pessôa, A. A. O. Filho, R. C. S. Sá
This study aimed to evaluate the in silico, in vitro, and ex-vivo toxicity of vitexin, the flavonoid 5,7,4'- trihydroxyflavone-8-C-β-glucopyranoside from Waltheria viscosissima. The chemical structure and predicted bioactive properties were also in silico analyzed. The in vitro and ex-vivo assays were performed according to the Ethics Code of the World Medical Association and were approved by the Ethics Committee of University Center of Patos (protocol number: 3.621.284). In silico analysis suggested that the molecule presents good oral bioavailability and good absorption; penetrating biological membranes. The toxicity tests revealed the potential effectiveness of the molecule in cellular protection against free radicals, in addition to possible antimutagenic, anticarcinogenic, antioxidant, antineoplastic, anti-inflammatory, anti-hemorrhagic and apoptosis agonist activity. Hemolytic and genotoxic assessment detected low hemolysis rates in human red blood cells and no cellular toxicity against oral mucosa cells. The data suggest that vitexin is a safe molecule for possible therapeutic application and its toxicity profile indicates viability for future studies.
本研究旨在评价牡荆素类黄酮5,7,4′-三羟基黄酮-8- c -β-葡萄糖吡喃苷的体内、体外和体外毒性。并对其化学结构和预测的生物活性进行了计算机分析。体外和离体试验按照世界医学协会伦理准则进行，并经帕托斯大学中心伦理委员会批准(协议号:3.621.284)。硅分析表明该分子具有良好的口服生物利用度和良好的吸收;穿透生物膜。毒性试验揭示了该分子对自由基的潜在保护作用，以及可能的抗诱变、抗癌、抗氧化、抗肿瘤、抗炎、抗出血和细胞凋亡激动剂活性。溶血和遗传毒性评估发现，人红细胞溶血率低，对口腔粘膜细胞无细胞毒性。这些数据表明牡荆素是一种安全的分子，可能用于治疗，其毒性谱显示了未来研究的可行性。
{"title":"In silico, in vitro and ex-vivo Toxicological Profiling of 5,7,4'-Trihydroxyflavone-8 -C-ß-Glucopyranoside - Vitexin","authors":"A. Sousa, Maria Denise Leite Ferreira, D. Fernandes, L. Cordeiro, M. F. Souza, H. Pessôa, A. A. O. Filho, R. C. S. Sá","doi":"10.4322/2179-443X.0709","DOIUrl":"https://doi.org/10.4322/2179-443X.0709","url":null,"abstract":"This study aimed to evaluate the in silico, in vitro, and ex-vivo toxicity of vitexin, the flavonoid 5,7,4'- trihydroxyflavone-8-C-β-glucopyranoside from Waltheria viscosissima. The chemical structure and predicted bioactive properties were also in silico analyzed. The in vitro and ex-vivo assays were performed according to the Ethics Code of the World Medical Association and were approved by the Ethics Committee of University Center of Patos (protocol number: 3.621.284). In silico analysis suggested that the molecule presents good oral bioavailability and good absorption; penetrating biological membranes. The toxicity tests revealed the potential effectiveness of the molecule in cellular protection against free radicals, in addition to possible antimutagenic, anticarcinogenic, antioxidant, antineoplastic, anti-inflammatory, anti-hemorrhagic and apoptosis agonist activity. Hemolytic and genotoxic assessment detected low hemolysis rates in human red blood cells and no cellular toxicity against oral mucosa cells. The data suggest that vitexin is a safe molecule for possible therapeutic application and its toxicity profile indicates viability for future studies.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"78 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84656543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daiane de Freitas Resende, G. C. S. Alves, R. O. Couto, Cristina Sanches, Farah Maria Drumond Chequer
Objective: To summarize evidence regarding the toxic potential of administering parabenscontaining cosmetics in humans. Methods: The systematic review followed the methodology proposed in Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA). Electronic searches of the PubMed, Virtual Health Library (BVS), and Science Direct databases were performed between October 1st and 31st, 2018. No language restriction was determined. Original articles reporting observational, in vitro and in silico studies of toxicity caused by parabens in human or human cells were considered for eligibility. Two independent reviewers performed data extraction and assessed the methodological quality and risk of bias of articles by using the Downs & Black Scale. Score levels greater than 70% were assumed to reflect good methodological quality. The Kappa coefficient was calculated. Results: A total of 254 studies were found. Following the eligibility evaluation, 22 studies were included for the qualitative synthesis. The concordance between the reviewers was substantial (Kappa coefficient = 0.650). The meaningful reported outcomes were: high concentrations of parabens in the body; apoptosis damage to sperm DNA; oxidative stress; DNA damage; irritative potential; interference in the control of adipogenesis; estrogenic activity; genotoxicity; necrosis; role in carcinogenesis of breast cancer; harmful effects on human skin when exposed to the sun; stimulation of oncogenes expression; and interference with DNA transcription. Despite most included articles presenting appreciable methodological quality, remarkable limitations were observed and the mechanisms by which parabens exert toxicity on humans remained unclear. Conclusions: The accumulation of parabens in the human organism following repeated cosmetics administration on the skin is noteworthy. However overall, the evidence so far does not make it possible to determine whether, and in what extent, the use of paraben-containing cosmetics can disturb human health. Further investigations are still required for clarifying these issues.
目的:总结有关对羟基苯甲酸酯类化妆品对人体潜在毒性的证据。方法:系统评价采用系统评价和元分析首选报告项目(PRISMA)中提出的方法。在2018年10月1日至31日期间对PubMed、Virtual Health Library (BVS)和Science Direct数据库进行了电子检索。没有确定语言限制。报告对羟基苯甲酸酯对人类或人类细胞毒性的观察性、体外和计算机研究的原创文章被认为是合格的。两名独立审稿人进行数据提取，并使用Downs & Black量表评估文章的方法学质量和偏倚风险。评分水平大于70%被认为反映了良好的方法学质量。计算Kappa系数。结果:共发现254项研究。在合格性评估后，纳入22项研究进行定性综合。审稿人之间的一致性很好(Kappa系数= 0.650)。有意义的报道结果是:体内高浓度的对羟基苯甲酸酯;精子DNA凋亡损伤;氧化应激;DNA损伤;刺激性的潜能;干预脂肪形成的控制;雌激素的活动;基因毒性;坏死;在乳腺癌发生中的作用;暴露在阳光下对人体皮肤的有害影响;刺激癌基因表达;干扰DNA转录。尽管大多数纳入的文章呈现出可观的方法学质量，但观察到显著的局限性，对羟基苯甲酸酯对人体产生毒性的机制仍不清楚。结论:对羟基苯甲酸酯在皮肤上反复使用化妆品后在人体体内的积累值得注意。然而，总的来说，到目前为止的证据还不能确定含有对羟基苯甲酸酯的化妆品是否会损害人体健康，以及在多大程度上损害人体健康。为了澄清这些问题，还需要进一步的调查。
{"title":"Can parabens be added to cosmetics without posing a risk to human health? A systematic review of its toxic effects","authors":"Daiane de Freitas Resende, G. C. S. Alves, R. O. Couto, Cristina Sanches, Farah Maria Drumond Chequer","doi":"10.4322/2179-443X.0706","DOIUrl":"https://doi.org/10.4322/2179-443X.0706","url":null,"abstract":"Objective: To summarize evidence regarding the toxic potential of administering parabenscontaining cosmetics in humans. Methods: The systematic review followed the methodology proposed in Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA). Electronic searches of the PubMed, Virtual Health Library (BVS), and Science Direct databases were performed between October 1st and 31st, 2018. No language restriction was determined. Original articles reporting observational, in vitro and in silico studies of toxicity caused by parabens in human or human cells were considered for eligibility. Two independent reviewers performed data extraction and assessed the methodological quality and risk of bias of articles by using the Downs & Black Scale. Score levels greater than 70% were assumed to reflect good methodological quality. The Kappa coefficient was calculated. Results: A total of 254 studies were found. Following the eligibility evaluation, 22 studies were included for the qualitative synthesis. The concordance between the reviewers was substantial (Kappa coefficient = 0.650). The meaningful reported outcomes were: high concentrations of parabens in the body; apoptosis damage to sperm DNA; oxidative stress; DNA damage; irritative potential; interference in the control of adipogenesis; estrogenic activity; genotoxicity; necrosis; role in carcinogenesis of breast cancer; harmful effects on human skin when exposed to the sun; stimulation of oncogenes expression; and interference with DNA transcription. Despite most included articles presenting appreciable methodological quality, remarkable limitations were observed and the mechanisms by which parabens exert toxicity on humans remained unclear. Conclusions: The accumulation of parabens in the human organism following repeated cosmetics administration on the skin is noteworthy. However overall, the evidence so far does not make it possible to determine whether, and in what extent, the use of paraben-containing cosmetics can disturb human health. Further investigations are still required for clarifying these issues.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"104 1","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85079613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. R. Momade, R. O. Vilhena, C. Castro, Fabiana Regis, Karime Domingues, Laís S. Schlichta, A. Cobre, R. Pontarolo
Objective. The aim of this study was the development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in commercial capsule formulation since pharmacopeias have not yet provided an official monograph for this drug. Methods. The parameters linearity, limit of detection, limit of quantitation, specificity, precision, accuracy, and robustness were determined according to Brazilian and international guidelines. Results. Linearity was determined for the analytical range of 5-15 μg/mL, and a limit of detection of 1.18 μg/mL and limit of quantification of 3.58 μg/mL were obtained. The method was selective and the precision was demonstrated through repeatability and intermediate precision, with relative standard deviations of 1.96% and 1.78%, respectively. In its turn, accuracy presented recovery percentages of 98.32-100.91%. All robustness and sample stability (48 h at 25 °C) results revealed no statistical variation among the groups. Conclusions. The presented method is suitable for the quantification of oclacitinib in commercial capsule formulation.
{"title":"Development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in capsule formulation","authors":"D. R. Momade, R. O. Vilhena, C. Castro, Fabiana Regis, Karime Domingues, Laís S. Schlichta, A. Cobre, R. Pontarolo","doi":"10.4322/2179-443X.0712","DOIUrl":"https://doi.org/10.4322/2179-443X.0712","url":null,"abstract":"Objective. The aim of this study was the development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in commercial capsule formulation since pharmacopeias have not yet provided an official monograph for this drug. Methods. The parameters linearity, limit of detection, limit of quantitation, specificity, precision, accuracy, and robustness were determined according to Brazilian and international guidelines. Results. Linearity was determined for the analytical range of 5-15 μg/mL, and a limit of detection of 1.18 μg/mL and limit of quantification of 3.58 μg/mL were obtained. The method was selective and the precision was demonstrated through repeatability and intermediate precision, with relative standard deviations of 1.96% and 1.78%, respectively. In its turn, accuracy presented recovery percentages of 98.32-100.91%. All robustness and sample stability (48 h at 25 °C) results revealed no statistical variation among the groups. Conclusions. The presented method is suitable for the quantification of oclacitinib in commercial capsule formulation.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"2 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81545121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriel Chaves Neto, H. Andrade, João Euclides Fernandes Braga, Lucindo J Q Junior, R. Almeida, M. M. Diniz
Anxiety in the world population has increased significantly; the problem has encouraged studies regarding innovative alternatives for treatment. Research with Citrus aurantium L. essential oil (CEO) has revealed positive results with anxiolytic effects in both animals and humans. However, certain limitations affect its storage and preservation, its efficiency in therapy, and determination of adequate posologies. The potential use of cyclodextrins as drug carriers has been successfully explored. This study aims to assess the anxiolytic potential of a CEO/2-hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex. Preparation of the inclusion complex was performed using the Alpha 1-2 LDplus lyophilizer. To allow formation, and avoid loss of volatiles to the atmosphere, Limonene (LIM), the main compound in CEO, together with HPβ-CD in a molar ratio of (1: 1M) was dispersed in ethanol for 36 hours using a laboratory shaker at room temperature (25°C). Non-clinical murine pharmacological tests were performed for anxiety assessment in experimental and control groups. To assess anxiety and motor impairment, the animals were evaluated using the elevated plus maze, open field, and rota-rod tests. Satisfactory results of the anxiolytic effect of the OEC complexed in HP-β-CD were observed, with the indication of an potentiation of the effect with doses lower than 500 mg/kg and 250 mg/kg complexed, suggesting improvement in the anxiolytic properties of the OEC.
{"title":"Non-clinical study of the anxiolytic effect of Citrus aurantium L. essential oil in an inclusion complex with 2-hydroxypropyl-ß-cyclodextrin","authors":"Gabriel Chaves Neto, H. Andrade, João Euclides Fernandes Braga, Lucindo J Q Junior, R. Almeida, M. M. Diniz","doi":"10.4322/2179-443X.0705","DOIUrl":"https://doi.org/10.4322/2179-443X.0705","url":null,"abstract":"Anxiety in the world population has increased significantly; the problem has encouraged studies regarding innovative alternatives for treatment. Research with Citrus aurantium L. essential oil (CEO) has revealed positive results with anxiolytic effects in both animals and humans. However, certain limitations affect its storage and preservation, its efficiency in therapy, and determination of adequate posologies. The potential use of cyclodextrins as drug carriers has been successfully explored. This study aims to assess the anxiolytic potential of a CEO/2-hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex. Preparation of the inclusion complex was performed using the Alpha 1-2 LDplus lyophilizer. To allow formation, and avoid loss of volatiles to the atmosphere, Limonene (LIM), the main compound in CEO, together with HPβ-CD in a molar ratio of (1: 1M) was dispersed in ethanol for 36 hours using a laboratory shaker at room temperature (25°C). Non-clinical murine pharmacological tests were performed for anxiety assessment in experimental and control groups. To assess anxiety and motor impairment, the animals were evaluated using the elevated plus maze, open field, and rota-rod tests. Satisfactory results of the anxiolytic effect of the OEC complexed in HP-β-CD were observed, with the indication of an potentiation of the effect with doses lower than 500 mg/kg and 250 mg/kg complexed, suggesting improvement in the anxiolytic properties of the OEC.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"os-50 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87360141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. M. Jerônimo, A. L. M. Viana, Miriam D. Fonseca, Marilene Lopes Ângelo, B. C. C. Salles, S. Duarte, S. Figueiredo, M. R. Rodrigues, F. Paula
The present study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8% w/v) by adult male Wistar rats submitted to high-intensity exercises. The parameters used in the evaluation were the determination of the activities of NADPH oxidase, myeloperoxidase and other antioxidant enzymes present in neutrophils of rats. It was observed that exercise-induced superoxide anion production depends on the NADPH oxidase activity (estimated by the cytochrome C reduction test) in peritoneal neutrophils (p < 0.05). The intake of caffeinated and decaffeinated instant coffee beverages and of a caffeine solution to 1.67% did not induced changes in myeloperoxidase, superoxide dismutase and glutathione peroxidase activities in the peritoneal neutrophils of rats after exercise (p < 0.05). From the obtained results, it was concluded that moderate intake of caffeinated instant coffee (equivalent to a daily human consumption of 4 50-mL cups of coffee) may have beneficial effects on health, contributing to a reduction in superoxide anion generation triggered in neutrophils after high-intensity exercise.
{"title":"Caffeinated instant coffee prevents an increase in exercise-mediated superoxide anion production in rat peritoneal neutrophils","authors":"E. M. Jerônimo, A. L. M. Viana, Miriam D. Fonseca, Marilene Lopes Ângelo, B. C. C. Salles, S. Duarte, S. Figueiredo, M. R. Rodrigues, F. Paula","doi":"10.4322/2179-443x.0673","DOIUrl":"https://doi.org/10.4322/2179-443x.0673","url":null,"abstract":"The present study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8% w/v) by adult male Wistar rats submitted to high-intensity exercises. The parameters used in the evaluation were the determination of the activities of NADPH oxidase, myeloperoxidase and other antioxidant enzymes present in neutrophils of rats. It was observed that exercise-induced superoxide anion production depends on the NADPH oxidase activity (estimated by the cytochrome C reduction test) in peritoneal neutrophils (p < 0.05). The intake of caffeinated and decaffeinated instant coffee beverages and of a caffeine solution to 1.67% did not induced changes in myeloperoxidase, superoxide dismutase and glutathione peroxidase activities in the peritoneal neutrophils of rats after exercise (p < 0.05). From the obtained results, it was concluded that moderate intake of caffeinated instant coffee (equivalent to a daily human consumption of 4 50-mL cups of coffee) may have beneficial effects on health, contributing to a reduction in superoxide anion generation triggered in neutrophils after high-intensity exercise.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"86 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78074500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loiane Massunari, A. C. A. Souza, Paula F. K. Domingues, Juhan Augusto Scardelato, L. V. Sacramento, E. Dezan-Júnior, C. Duque
Herbal medicines have been studied and potential antimicrobial agents emerging as treatment against oral diseases. This study evaluated the antimicrobial activity of the Mikania laevigata (ML) and Cordia verbenacea (CV) crude extract and fractions against oral pathogens associated with persistent dental root infections, under planktonic and biofilm conditions. Minimal inhibitory concentrations and minimal bactericidal/fungicidal concentrations were determined for the ML and CV fractions/extracts against Enterococcus faecalis, Actinomyces israelii, Pseudomonas aeruginosa and Candida albicans using the microdilution method. The best results were chosen for subsequent biofilm assays. All tested ML and CV extracts/fractions had inhibitory activity against E. faecalis and A. israelii. ML ethyl acetate fraction affected the growth of all microorganisms tested. C. albicans and P. aeruginosa were not affected by any CV extract/fraction. ML ethyl acetate fraction eliminated E. faecalis, A. israelii and P. aeruginosa biofilms after 24h. Similar result was observed for ML crude hydroethanolic extract and its hexane fraction for A. israelii. CV hexane fraction was able to eliminate A. israelli biofilms. None of the tested extracts or fractions eliminated C. albicans biofilm. Mikania laevigata ethyl acetate fraction is an efficient antimicrobial agent against oral pathogens and could be indicated for the treatment of persistent dental infections.
{"title":"Inhibitory activity of Varronia curassavica and Mikania laevigata fractions against pathogens associated with persistent dental infections","authors":"Loiane Massunari, A. C. A. Souza, Paula F. K. Domingues, Juhan Augusto Scardelato, L. V. Sacramento, E. Dezan-Júnior, C. Duque","doi":"10.4322/2179-443x.0686","DOIUrl":"https://doi.org/10.4322/2179-443x.0686","url":null,"abstract":"Herbal medicines have been studied and potential antimicrobial agents emerging as treatment against oral diseases. This study evaluated the antimicrobial activity of the Mikania laevigata (ML) and Cordia verbenacea (CV) crude extract and fractions against oral pathogens associated with persistent dental root infections, under planktonic and biofilm conditions. Minimal inhibitory concentrations and minimal bactericidal/fungicidal concentrations were determined for the ML and CV fractions/extracts against Enterococcus faecalis, Actinomyces israelii, Pseudomonas aeruginosa and Candida albicans using the microdilution method. The best results were chosen for subsequent biofilm assays. All tested ML and CV extracts/fractions had inhibitory activity against E. faecalis and A. israelii. ML ethyl acetate fraction affected the growth of all microorganisms tested. C. albicans and P. aeruginosa were not affected by any CV extract/fraction. ML ethyl acetate fraction eliminated E. faecalis, A. israelii and P. aeruginosa biofilms after 24h. Similar result was observed for ML crude hydroethanolic extract and its hexane fraction for A. israelii. CV hexane fraction was able to eliminate A. israelli biofilms. None of the tested extracts or fractions eliminated C. albicans biofilm. Mikania laevigata ethyl acetate fraction is an efficient antimicrobial agent against oral pathogens and could be indicated for the treatment of persistent dental infections.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"86 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80783455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Palma, T. L. Sales, G. C. S. Alves, S. Fook, Alba Otoni, Cristina Sanches, Farah Maria Drumond Chequer
Intoxications represent a serious public health problem. According to the World Health Organization, 193,000 deaths per year are caused by unintended intoxications worldwide. This study aims to know the profile of intoxications at the 24-hour Emergency Service Unit in Divinopolis, in the state of Minas Gerais. This is a descriptive study in which the study population chosen were all patients treated for intoxications in the period from 2017 to 2018, registered in the health service; 421 cases of intoxications were recorded. The female gender was the most prevalent, and the age group with the most cases was 21 to 30 years. The main circumstance found was attempted suicide (83.1 %), and the main causative agent was medication (76.5 %), with benzodiazepines being the main class responsible for intoxications. The most used treatment was gastric lavage and activated charcoal. This study demonstrates that knowledge about the city's intoxication profile is important for the development of treatment and patient management methods to better suit the demands of the municipality.
{"title":"Profile of intoxications served at the 24-hour emergency service unit","authors":"A. Palma, T. L. Sales, G. C. S. Alves, S. Fook, Alba Otoni, Cristina Sanches, Farah Maria Drumond Chequer","doi":"10.4322/2179-443x.0680","DOIUrl":"https://doi.org/10.4322/2179-443x.0680","url":null,"abstract":"Intoxications represent a serious public health problem. According to the World Health Organization, 193,000 deaths per year are caused by unintended intoxications worldwide. This study aims to know the profile of intoxications at the 24-hour Emergency Service Unit in Divinopolis, in the state of Minas Gerais. This is a descriptive study in which the study population chosen were all patients treated for intoxications in the period from 2017 to 2018, registered in the health service; 421 cases of intoxications were recorded. The female gender was the most prevalent, and the age group with the most cases was 21 to 30 years. The main circumstance found was attempted suicide (83.1 %), and the main causative agent was medication (76.5 %), with benzodiazepines being the main class responsible for intoxications. The most used treatment was gastric lavage and activated charcoal. This study demonstrates that knowledge about the city's intoxication profile is important for the development of treatment and patient management methods to better suit the demands of the municipality.","PeriodicalId":21209,"journal":{"name":"Revista de Ciências Farmacêuticas Básica e Aplicada","volume":"105 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80761758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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