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RNA-Seq study of in vivo-produced single male and female bovine blastocysts 体内产生的单雄性和雌性牛囊胚的RNA-Seq研究
Pub Date : 2023-01-01 DOI: 10.37496/rbz5220220137
Baoshan Cui, Jie Wang, Fei Huang, Nan Li, Qinghua Gao
The objective of this study was to identify differentially expressed genes (DEG) between females and males in early bovine embryos. Superovulated cattle (n = 4) in the age span of 16–18 months were artificially inseminated with semen from the same bull that has been proven to be fertile. Blastocysts were collected by routine non-surgical uterine flushing on day 7 after insemination. This study determines the sex of embryos using a micro-injection pipette to collect a few blastomeres through the [...]
本研究的目的是鉴定早期牛胚胎中雌性和雄性之间的差异表达基因(DEG)。将年龄在16-18个月的超排卵牛(n = 4)用已被证明具有生育能力的同一头牛的精液进行人工授精。在授精后第7天采用常规非手术子宫冲洗收集囊胚。本研究使用微注射移液管收集几个卵裂球,通过[…]
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Revista Brasileira De Zootecnia
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