Biosorption has been known as a potential method in reducing heavy metals and dyes in wastewater, including chrome (Cr) and dyes contents of the wastewater from the batik industry. Straw and spent mushroom compost (SMC) are potential biosorbents due to cheap, abundant, and easily obtained. However, the effectivity of the biosorbents is not only depended upon the type of material but also their surface area. In this study, a modified tea bag was used to increase the surface area of the straw and spent mushroom compost to absorb Cr and dyes from the batik industry wastewater. The optimum of biosorbent ratio and pH in Cr absorption and dyes decolorization was measured. This experiment was conducted using Spilt Plot Design. The results showed that the highest Cr absorption was found at 0,0050 mg/g, and the percentage of decolorization was 68,92% in the biosorbent ratio of 3:1 and pH 5. Biosorbent packed in the tea bags modification was effective removes Cr and dyes in the batik industry wastewater.
{"title":"Biosorption chrome (Cr) and dyes using biosorbent in the modified tea bag","authors":"S. Lestari, R. S. Dewi, E. Wibowo, Atang","doi":"10.37604/jmsb.v1i1.20","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.20","url":null,"abstract":"Biosorption has been known as a potential method in reducing heavy metals and dyes in wastewater, including chrome (Cr) and dyes contents of the wastewater from the batik industry. Straw and spent mushroom compost (SMC) are potential biosorbents due to cheap, abundant, and easily obtained. However, the effectivity of the biosorbents is not only depended upon the type of material but also their surface area. In this study, a modified tea bag was used to increase the surface area of the straw and spent mushroom compost to absorb Cr and dyes from the batik industry wastewater. The optimum of biosorbent ratio and pH in Cr absorption and dyes decolorization was measured. This experiment was conducted using Spilt Plot Design. The results showed that the highest Cr absorption was found at 0,0050 mg/g, and the percentage of decolorization was 68,92% in the biosorbent ratio of 3:1 and pH 5. Biosorbent packed in the tea bags modification was effective removes Cr and dyes in the batik industry wastewater.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115757522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The areas surrounding peat swamp forest at Dusun Meliau and Bukit Peninjau, Kapuas Hulu, West Kalimantan host a diverse species of flora and fauna, including macrofungi. However, the information on the macrofungal diversity in this area has not been reported. The goal of this study was to obtain information on the diversity of macrofungi that can be used as food and medicine. Data was collected by direct modified collection method using plot (10 × 20 m) with interval 10 m along the transect line. A total of 79 specimens of macrofungi discovered from the two locations, of which 23 and 66 specimens were collected from Dusun Meliau and Bukit Peninjau, respectively. Of the 79 macrofungi specimens, 16 specimens were potential as a food source, and 41 as medicine. The macrofungi in this study was dominated by members of Ordo Aphyllophorales.
在西加里曼丹的Dusun Meliau和Bukit Peninjau, Kapuas Hulu的泥炭沼泽森林周围地区拥有多种动植物，包括大型真菌。然而，有关该地区大型真菌多样性的信息尚未见报道。本研究的目的是获取可用于食品和药物的大型真菌的多样性信息。数据采集采用直接修正采集法，样线为10 × 20 m，采样间隔为10 m。在两个地点共发现大型真菌标本79份，其中独顺美柳和武吉Peninjau分别有23份和66份。79份大型真菌标本中，16份具有潜在的食物来源，41份具有药用价值。本研究的大型真菌以Ordo Aphyllophorales的成员为主。
{"title":"Inventory of macrofungi at peat swamp forest area, Kapuas Hulu, West Kalimantan","authors":"Noverita, T. Setia","doi":"10.37604/jmsb.v1i1.17","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.17","url":null,"abstract":"The areas surrounding peat swamp forest at Dusun Meliau and Bukit Peninjau, Kapuas Hulu, West Kalimantan host a diverse species of flora and fauna, including macrofungi. However, the information on the macrofungal diversity in this area has not been reported. The goal of this study was to obtain information on the diversity of macrofungi that can be used as food and medicine. Data was collected by direct modified collection method using plot (10 × 20 m) with interval 10 m along the transect line. A total of 79 specimens of macrofungi discovered from the two locations, of which 23 and 66 specimens were collected from Dusun Meliau and Bukit Peninjau, respectively. Of the 79 macrofungi specimens, 16 specimens were potential as a food source, and 41 as medicine. The macrofungi in this study was dominated by members of Ordo Aphyllophorales.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121405674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.
{"title":"Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana","authors":"I. Hidayat, Lita Citra Dewi, D. Sukmawati","doi":"10.37604/jmsb.v1i1.16","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.16","url":null,"abstract":"Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126701247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biofilm is a structural form of a microbial group that is protected by the Extracellular Polymeric Substance (EPS) matrix. The biofilm is considered as the main mediator of infection, and plays a major role in the occurrence of drug resistance. This study was aimed at determining the antimicrobial and antibiofilm activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. The antimicrobial assay showed that papaya leaf extract exhibits higher activity compared to stevia leaf extract in inhibiting the growth of the biofilm-forming bacteria. The optimum condition of papaya leaf extract to inhibit biofilm-forming bacterial growth occurred at 45% and 75% concentrations of the extract (pH 7). A 100% biofilm degradation by papaya leaf extract occurred at pH 6 and pH 9.
{"title":"Antibiofilm and antimicrobial activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria","authors":"A. Hastuty","doi":"10.37604/jmsb.v1i1.18","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.18","url":null,"abstract":"Biofilm is a structural form of a microbial group that is protected by the Extracellular Polymeric Substance (EPS) matrix. The biofilm is considered as the main mediator of infection, and plays a major role in the occurrence of drug resistance. This study was aimed at determining the antimicrobial and antibiofilm activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. The antimicrobial assay showed that papaya leaf extract exhibits higher activity compared to stevia leaf extract in inhibiting the growth of the biofilm-forming bacteria. The optimum condition of papaya leaf extract to inhibit biofilm-forming bacterial growth occurred at 45% and 75% concentrations of the extract (pH 7). A 100% biofilm degradation by papaya leaf extract occurred at pH 6 and pH 9.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122584972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.
{"title":"PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer","authors":"I. Hidayat, N. Laili, D. Agustiyani, S. Antonius","doi":"10.37604/jmsb.v1i1.21","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.21","url":null,"abstract":"Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130841456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Latifah, I. Idris, T. P. Napitupulu, A. Z. N. Ikhwani, Gunawan Ruhiyat, I. N. Sumerta, A. Kanti, F. Y. Amandita, I. Sudiana
Symbiosis of and AMF increase soybean production, but the information on the association between these microbes in Paraserianthes falcataria (L.) I.C. Nielson or ‘sengon’ under Hg contamination is limited. We observed P. falcataria inoculated with arbuscular mycorrhiza fungi (Glomus sp.) stimulate nodule formation under Hg contamination. The study was set up in a pot experiment in the growth chamber a the Hg concentration was adjusted to 50 ppm in soil medium. Inoculation of AMF stimulates nodule formation and increases P. falcataria tolerance to Hg. We isolated several bacteria from the rhizosphere belonging to Rhizobium group and others. Understanding the ecology of soil bacteria is important for Hg bioremediation using P. falcataria.
{"title":"Mycorrhiza stimulates Rhizobium infection in Paraserianthes falcataria (L.) I.C. Nielson under Hg contamination","authors":"I. Latifah, I. Idris, T. P. Napitupulu, A. Z. N. Ikhwani, Gunawan Ruhiyat, I. N. Sumerta, A. Kanti, F. Y. Amandita, I. Sudiana","doi":"10.37604/jmsb.v3i1.70","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.70","url":null,"abstract":"Symbiosis of and AMF increase soybean production, but the information on the association between these microbes in Paraserianthes falcataria (L.) I.C. Nielson or ‘sengon’ under Hg contamination is limited. We observed P. falcataria inoculated with arbuscular mycorrhiza fungi (Glomus sp.) stimulate nodule formation under Hg contamination. The study was set up in a pot experiment in the growth chamber a the Hg concentration was adjusted to 50 ppm in soil medium. Inoculation of AMF stimulates nodule formation and increases P. falcataria tolerance to Hg. We isolated several bacteria from the rhizosphere belonging to Rhizobium group and others. Understanding the ecology of soil bacteria is important for Hg bioremediation using P. falcataria.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116073634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Fibriani, Irin Annisa Evitayani, Gusti Ayu Prani Pradani, Rebecca Stephanie, Ema Rahmawati, R. B. Ristandi, Cut Nur Cinthia Alamanda, Rifky Waluyajati Rachman, Rini Robiani, Isak Solihin
According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.
{"title":"Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java","authors":"A. Fibriani, Irin Annisa Evitayani, Gusti Ayu Prani Pradani, Rebecca Stephanie, Ema Rahmawati, R. B. Ristandi, Cut Nur Cinthia Alamanda, Rifky Waluyajati Rachman, Rini Robiani, Isak Solihin","doi":"10.37604/jmsb.v3i1.66","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.66","url":null,"abstract":"According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114101212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants
{"title":"Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling","authors":"I. Isti'anah, Nisa Rachmania, A. Tjahjoleksono","doi":"10.37604/jmsb.v3i1.77","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.77","url":null,"abstract":"Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114988200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arif Nurkanto, R. Handayani, I. Purnaningsih, Mia Kusmiati, Ninu Setianingrum, M. Mulyadi, Endang Kusdiyanti, A. Dinoto
In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m
{"title":"Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media","authors":"Arif Nurkanto, R. Handayani, I. Purnaningsih, Mia Kusmiati, Ninu Setianingrum, M. Mulyadi, Endang Kusdiyanti, A. Dinoto","doi":"10.37604/jmsb.v3i1.61","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.61","url":null,"abstract":"In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"287 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121070356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.
{"title":"Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro","authors":"K. Khusnul, Puput Putuh Anisa, D. P. Virgianti","doi":"10.37604/jmsb.v3i1.78","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.78","url":null,"abstract":"One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122028763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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