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Isolation and molecular characterization of ginger soft rot pathogenic fungi in Aizawl district of Mizoram, India 印度米佐拉姆邦Aizawl地区生姜软腐病病原真菌的分离与分子鉴定
Pub Date : 2018-12-31 DOI: 10.33493/SCIVIS.18.04.02
G. Rosangkima, Vanramliana, H. Lalthanzara, Lalringngheti, H. Lalramnghaki
Ginger soft rot disease caused by fungal pathogens have become one of the most serious problems causing reduced production around the world. It has also caused a major problem among farmers of Mizoram state in India resulting in a huge decline in rhizome yield. However, the exact causative agents of this disease have not been identified in the state. Therefore, the present study was undertaken to isolate and characterized the causative agents of ginger soft rot disease from the diseased plants collected from five different villages of Aizawl district, Mizoram. Isolated fungi were cultured and morphological and molecular identification were done using internal transcribed spacer of rDNA. Fusarium oxysporum, F. solani and Plectosphaerella cucumerina were identified in ginger samples from five villages. Fusarium spp. were the most common and seem to be the major causative agents. It is suggested that further investigation is required to explore the diversity of ginger soft rot pathogenic fungi in the whole state which could be helpful in introducing effective and eco-friendly disease management programme.
由真菌病原菌引起的生姜软腐病已成为世界范围内导致生姜减产的最严重问题之一。它还在印度米佐拉姆邦的农民中造成了一个重大问题,导致根茎产量大幅下降。然而,这种疾病的确切病原体尚未在该州确定。因此,本研究从印度米佐拉姆邦Aizawl地区5个不同村庄的生姜病株中分离和鉴定了姜软腐病的病原。对分离真菌进行培养,利用rDNA内转录间隔段进行形态学和分子鉴定。在5个村的生姜样品中检出尖孢镰刀菌、茄枯镰刀菌和黄瓜枯萎镰刀菌。镰刀菌是最常见的,似乎是主要的病原体。建议对全国生姜软腐病病原真菌的多样性进行深入调查,为制定有效的生态友好型病害管理方案提供依据。
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引用次数: 4
Phytochemical screening and evaluation of antidepressant activity of methanolic extract of the spadix of Colocasia affinis Schott 番槐叶柄甲醇提取物的植物化学筛选及抗抑郁活性评价
Pub Date : 2018-12-31 DOI: 10.33493/scivis.18.04.01
C. Lalremruati, C. Malsawmtluangi, H. Lalhlenmawia
The objective of this study was to determine the phytoconstituents present in successive extracts and the antidepressant activity of methanolic extract of the spadix of Colocasia affinis. The preliminary phytochemical investigation indicated the presence of fats and fixed oils, steroids and triterpenoids in petroleum ether extract, steroids and triterpenoids in chloroform extract, flavonoids and tannins in methanol extract and carbohydrates in the aqueous extract. An in vivo test for antidepressant activity was performed on the methanolic extract of the plant at two different doses using force swim test and tail suspension test on an experimental animal model (rat). Imipramine was used as a standard drug for the study. The methanolic extract at 200 and 400 mg/kg, produced significant reduction (p<0.001) in the immobility period when compared with that of control group animals in both the tests. Thus, the result indicates that the methanolic extract exhibited significantly good antidepressant activity. Further investigation may be needed to understand the actual mechanism of action.
本研究的目的是确定附着物叶柄甲醇提取物中存在的植物成分和抗抑郁活性。初步的植物化学研究表明,石油醚提取物中含有脂肪和固定油,氯仿提取物中含有类固醇和三萜,甲醇提取物中含有黄酮类和单宁,水提取物中含有碳水化合物。在实验动物模型(大鼠)上,采用力游试验和悬尾试验对两种不同剂量的植物甲醇提取物进行了体内抗抑郁活性试验。丙咪嗪被用作该研究的标准药物。与对照组相比,200和400 mg/kg的甲醇提取物在静止期均显著降低(p<0.001)。因此,结果表明,甲醇提取物具有显著的抗抑郁活性。可能需要进一步的研究来了解实际的作用机制。
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引用次数: 1
Some phytochemical analyses of different extracts of the cogon grass Imperata cylindrica from Mizoram, India 印度米佐拉姆邦白茅不同提取物的植物化学分析
Pub Date : 2018-12-31 DOI: 10.33493/SCIVIS.18.04.03
P. Lalthanpuii, Zarzokimi, K. Lalchhandama
Cogon grass, Imperata cylindrica (L.) Räuschel, was studied for its flavonoid and phenol contents. A series of extracts of the underground (rhizome-root) parts was prepared by hot extraction using solvents of different polarities such as chloroform, methanol, and petroleum ether. The total flavonoid content of the plant extracts was determined based on the reaction with aluminum chloride, sodium nitrite, and sodium hydroxide. Quercetin was used as a standard reference. Chloroform extract showed highest content of flavonoids with 30.88 mg QE/g dry wt., followed by petroleum ether extract which was 22.05 mg mg QE/g dry wt., and methanol extract has the least value with 7.35 mg QE/g dry wt. The total phenolic content of the plant was estimated based on the reaction of Folin-Ciocalteu reagent using gallic acid as the standard reference. Again, the chloroform extract had the highest content with 7.54 mg GAE/g dry wt., while methanol extract contained 5.03 mg GAE/g dry wt., and petroleum ether extract contained 3.63 mg mg GAE/g dry wt. Our study shows that I. cylindrica is a good source of antioxidants.
白茅,白茅(L.)以Räuschel为原料,对其黄酮和酚含量进行了研究。以氯仿、甲醇、石油醚等不同极性溶剂为溶剂,采用热萃取法制备了一系列地下部分(根茎-根)提取物。通过与氯化铝、亚硝酸钠和氢氧化钠的反应,测定了植物提取物的总黄酮含量。槲皮素为标准参比物。氯仿提取物总黄酮含量最高,为30.88 mg QE/g干wt,其次为石油醚提取物,为22.05 mg QE/g干wt,甲醇提取物含量最低,为7.35 mg QE/g干wt。以没食子酸为标准参比物,通过与Folin-Ciocalteu试剂反应,估算出该植物总酚含量。同样,氯仿提取物的GAE含量最高,为7.54 mg /g干wt,甲醇提取物的GAE含量为5.03 mg /g干wt,石油醚提取物的GAE含量为3.63 mg /g干wt。
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引用次数: 5
Diversity, distribution and conservation status of fish of the genus Garra (Cypriniformes: Cyprinidae) from the Kaladan drainage of Mizoram, northeastern India 印度东北部米佐拉姆邦卡拉丹流域加拉鱼属鱼类的多样性、分布和保护现状
Pub Date : 2018-12-31 DOI: 10.33493/SCIVIS.18.04.04
B. Solo, Lalnuntluanga
The present study was carried out on the diversity of fish under the genus Garra from the Kaladan River and its tributaries of Mizoram, northeast India. The study reported the occurrence of seven species viz. Garra flavatra, G. cf. koladynensis, G. cf. matensis, G. nigricolis, G. rakhinica, G. manipurensis and G. khawbungi. Key to Garra species from Kaladan River drainage of Mizoram and their brief descriptions are given.
本研究对来自印度东北部米佐拉姆邦卡拉丹河及其支流的加拉属鱼类的多样性进行了研究。本研究报告了7种黄芪(Garra flavatra)、G. cf. koladynensis、G. cf. matensis、G. nigricolis、G. rakhinica、G. manipurensis和G. khawbungi。米佐拉姆邦卡拉丹河流域加拉属植物的分类及其简要描述。
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引用次数: 1
In vitro antioxidant and preliminary phytochemical screening of methanolic extract of Macaranga denticulata (Blume) Müll.Arg. 黄芩醇提物体外抗氧化及植物化学初步筛选。
Pub Date : 2018-09-30 DOI: 10.33493/scivis.18.03.02
R. Lalmuanawmi, Zothanpuia
Macaranga denticulata (Blume) Müll.Arg. (family Euphorbiaceae) is an evergreen tree and a common pioneer species in moist open and secondary forest. It is commonly known as Kharpa in Mizoram. Traditionally, the species of Macaranga are used in the treatment of swelling, cuts, sores, boils and bruises. Preliminary phytochemical screening and evaluation of in vitro antioxidant activity were carried out on the methanolic extract obtained from the bark of M. denticulata. The presence of alkaloids, tannins, flavonoids, saponins, steroids and triterpenoids was indicated by the tests conducted. The in vitro antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and hydrogen peroxide radical scavenging activity. Ascorbic acid and butylated hydroxytoluene (BHT) were used as reference standards. The methanolic extract of the plant shows a strong antioxidant activity comparable to that of the reference standards.
小檗属植物(umaranga denticulata);大戟科(Euphorbiaceae)是一种常绿乔木,是湿阔叶林和次生林中常见的先锋树种。在米佐拉姆邦,它通常被称为Kharpa。传统上,Macaranga被用于治疗肿胀、割伤、疮、疖子和瘀伤。对小针叶树皮甲醇提取物进行了初步的植物化学筛选和体外抗氧化活性评价。通过测试表明,其中含有生物碱、单宁、黄酮类、皂苷、类固醇和三萜。以2,2 -二苯基-1-苦味酰肼(DPPH)自由基清除能力、还原能力和过氧化氢自由基清除能力为指标评价其体外抗氧化活性。以抗坏血酸和丁基羟基甲苯(BHT)为对照品。该植物的甲醇提取物显示出与参考标准相当的强抗氧化活性。
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引用次数: 0
Some pharmacognostic studies of the cogon grass Imperata cylindrica from Mizoram, India 印度米佐拉姆邦白茅的生药学研究
Pub Date : 2018-09-30 DOI: 10.33493/SCIVIS.18.03.03
P. Lalthanpuii, Zarzokimi, K. Lalchhandama
Cogon grass Imperata cylindrica is a perennial grass belonging to the family Poaceae, and the rhizome-root portion of which is used for the treatment of bacterial infections, ringworms and other skin infections. Among the Mizo people they are directly consumed or juiced for the treatment of intestinal infection. Its chemical and biological properties are poorly documented. In this study, a methanol extract of the rhizome-root was prepared by hot extraction in a Soxhlet apparatus. Standard chemical tests were conducted. The presence of alkaloids, carbohydrates including reducing sugars, phytosterols, tannins, saponins and proteins were confirmed as the major bio-compounds. Free radical-scavenging activities were also determined. The plant extract indicated concentration-dependent scavenging activity on DPPH with an inhibitory concentration (IC50) of 2.14 µg/ml. H2O2 was similarly scavenged, in which the IC50 was 2.221 µg/ml. Our results suggest that I. cylindrica has important medicinal values.
白茅是一种多年生草本植物,属于禾本科,其根茎部分用于治疗细菌感染,癣和其他皮肤感染。在米佐族人中,他们直接食用或榨汁治疗肠道感染。其化学和生物学特性文献记载甚少。在索氏装置中,采用热萃取法制备了根状茎的甲醇提取物。进行了标准化学试验。生物碱、碳水化合物(包括还原糖)、植物甾醇、单宁、皂苷和蛋白质被确认为主要的生物化合物。还测定了自由基清除活性。该植物提取物对DPPH的清除活性呈浓度依赖性,抑制浓度(IC50)为2.14µg/ml。H2O2同样被清除,IC50为2.221µg/ml。提示白茅具有重要的药用价值。
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引用次数: 0
Photocatalytic degradation of methylene blue from aqueous solutions using nanopillars-TiO2 thin films: Batch reactor studies 纳米柱- tio2薄膜光催化降解水溶液中的亚甲基蓝:间歇反应器研究
Pub Date : 2018-09-30 DOI: 10.33493/SCIVIS.18.03.01
C. Lalhriatpuia
Nanopillars-TiO2 thin films was obtained on a borosilicate glass substrate with (S1) and without (S2) polyethylene glycol as template. The photocatalytic behaviour of S1 and S2 thin films was assessed inthe degradation of methylene blue (MB) dye from aqueous solution under batch reactor operations. The thin films were characterized by the SEM, XRD, FTIR and AFM analytical methods. BET specific surface area and pore sizes were also obtained. The XRD data confirmed that the TiO2 particles are in its anatase mineral phase. The SEM and AFM images indicated the catalyst is composed with nanosized pillars of TiO2, evenly distributed on the surface of the substrate. The BET specific surface area and pore sizes of S1 and S2 catalyst were found to be 5.217 and 1.420 m2/g and 7.77 and 4.16 nm respectively. The photocatalytic degradation of MB was well studied at wide range of physico-chemical parameters. The effect of solution pH (pH 4.0 to 10.0) and MB initial concentration (1.0 to 10.0 mg/L) was extensively studied and the effect of several interfering ions, i.e., cadmium nitrate, copper sulfate, zinc chloride, sodium chloride, sodium nitrate, sodium nitrite, glycine, oxalic acid and EDTA in the photocatalytic degradation of MB was demonstrated. The maximum percent removal of MB was observed at pH 8.0 beyond which it started decreasing and a low initial concentration of the pollutant highly favoured the photocatalytic degradation using thin films and the presence of several interfering ions diminished the photocatalytic activity of thin films to some extent. The overall photocatalytic activity was in the order: S2 > S1 > UV. The photocatalytic degradation of MB was followed the pseudo-first-order rate kinetics. The mineralization of MB was studied with total organic carbon measurement using the TOC (total organic carbon) analysis.
以(S1)和(S2)聚乙二醇为模板,在硼硅酸盐玻璃衬底上制备了纳米柱- tio2薄膜。在间歇反应器操作下,研究了S1和S2薄膜在降解亚甲基蓝(MB)染料中的光催化性能。采用SEM、XRD、FTIR和AFM等分析方法对薄膜进行了表征。得到了BET比表面积和孔径。XRD数据证实TiO2颗粒处于锐钛矿相。SEM和AFM分析表明,催化剂由TiO2纳米柱组成,均匀分布在衬底表面。S1和S2催化剂的BET比表面积和孔径分别为5.217和1.420 m2/g和7.77和4.16 nm。在较宽的理化参数范围内,对光催化降解MB进行了较好的研究。广泛研究了溶液pH (pH 4.0 ~ 10.0)和MB初始浓度(1.0 ~ 10.0 mg/L)对光催化降解MB的影响,并考察了硝酸镉、硫酸铜、氯化锌、氯化钠、硝酸钠、亚硝酸钠、甘氨酸、草酸和EDTA等干扰离子对光催化降解MB的影响。pH值为8.0时,MB去除率达到最大值,超过该值后MB去除率开始下降,较低的初始浓度有利于薄膜的光催化降解,一些干扰离子的存在在一定程度上降低了薄膜的光催化活性。总光催化活性顺序为:S2 > S1 > UV。光催化降解MB符合准一级速率动力学。用TOC (total organic carbon,总有机碳)法测定了MB的矿化。
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引用次数: 3
The saga of scrub typhus with a note on the outbreaks in Mizoram 丛林斑疹伤寒的传奇故事,并说明了米佐拉姆邦的疫情
Pub Date : 2018-06-30 DOI: 10.33493/SCIVIS.18.02.01
K. Lalchhandama
Scrub typhus is one the most important re-emerging infectious disease, and perhaps, the most important bacterial disease. Caused by Orientia tsutsugamushi, it is transmitted through the bite of mites belonging to the genus Leptotrombidium, in which the bacteria are obligate parasites. Though the mites are natural ectoparasites of rodents and other animals, in which there is no disease, opportunistic infection to humans gives rise to a serious disease. Known to Japanese physicians as tsutsugamushi (insect disease), human infection is caused by the larvae of trombiculid mites, the fact established by Mataro Nagayo and co-workers established in 1917. The pathogen was discovered by Naosuke Hayashi in 1920. In Mizoram, the disease has been rampant since 2011. This paper summarises available data on the prevalence of the infection in different districts base on collective information from various sources. Records between 2012 and 2018 show that over a thousand people had been infected and 35 people had died of the disease.
恙虫病是最重要的再发传染病之一,也许也是最重要的细菌性疾病。由恙虫病东方体引起,通过细原体属螨的叮咬传播,其中的细菌是专性寄生虫。虽然螨虫是啮齿类动物和其他动物的天然体外寄生虫,没有疾病,但机会性感染人类会引起严重疾病。日本医生将其称为恙虫病(昆虫疾病),人类感染是由恙虫螨的幼虫引起的,这一事实由Mataro Nagayo及其同事于1917年确定。这种病原体是由林直介于1920年发现的。在米佐拉姆邦,这种疾病自2011年以来一直猖獗。本文根据各种来源的集体信息,总结了不同地区感染流行的现有数据。2012年至2018年的记录显示,已有1000多人感染这种疾病,35人死于这种疾病。
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引用次数: 3
Estimation of current population mean using two-occasion successive sampling with one auxiliary variable 用一个辅助变量的两次连续抽样估计当前总体均值
Pub Date : 2018-06-30 DOI: 10.33493/SCIVIS.18.02.05
R. Zoramthanga
In this study, two-occasion successive sampling for ratio-to-regression estimator was used to determine the current estimate of the population mean using only the matched part and one auxiliary variable, which is available on both the occasions. The data used were based on the total number of female workers in villages in Mizoram with the total number of literate female in villages in Mizoram as an auxiliary variables. The data were gotten from Census of India 2001 and 2011. The optimum mean square error of the combined ratio-to-regression and ratio estimator has been compared with (i) the optimum mean square error of the chain-type ratio estimator (ii) mean per unit estimator and (iii) combined estimator when no auxiliary information is used at any occasion. This result showed that the combined ratio-to-regression and ratio estimator is more efficient than the other three existing estimators.
在本研究中,采用两次连续抽样的比率-回归估计器,仅使用匹配部分和一个辅助变量来确定总体均值的当前估计值,这两种情况下都可用。所使用的数据基于米佐拉姆邦村庄女工总数,米佐拉姆邦村庄识字女性总数作为辅助变量。数据来自2001年和2011年的印度人口普查。将组合比率回归和比率估计器的最佳均方误差与(i)链式比率估计器的最佳均方误差(ii)单位平均估计器和(iii)在任何场合不使用辅助信息时的组合估计器进行了比较。结果表明,比值回归和比值估计相结合的方法比其他三种估计方法更有效。
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引用次数: 0
Comparison of single and double entry twin cup dosimeter in measuring indoor radon and thoron concentration in Mizoram, India 单入口和双入口双杯剂量计在印度米佐拉姆邦测量室内氡和钍浓度的比较
Pub Date : 2018-06-30 DOI: 10.33493/scivis.18.02.02
L. Chhangte, P. C. Rohmingliana, B. Sahoo, B. K. Sapra, Vanramlawma, Hmingchungnunga, Remlalsiama, Z. Pachuau, B. Zoliana
Indoor radon and thoron concentrations have been measured using solid state nuclear track detector (LR-115 type-ІІ) based twin cup dosimeter with single and double entry deployed side by side. The measurements have been carried out in 50 dwellings of 8 different villages/towns situated in Saiha and Lawngtlai districts, Mizoram. Dwellings were selected primarily from the gamma level measured using Micro-R survey meter and the construction type of the building. The average concentrations of radon and thoron were found to be 75.76 Bq/m3 and 96.50 Bq/m3 for single entry dosimeter and 63.47 Bq/m3 and 19.79 Bq/m3 for double entry dosimeter. The single entry dosimeter was found to give more reliable observations than that of double entry in terms of trends of seasonal data and also on the theory behind the manufacturing qualities.
室内氡和钍浓度已使用固态核径迹探测器(LR-115型-ІІ)双杯剂量计进行测量,单入口和双入口并排部署。这些测量是在米佐拉姆邦Saiha和lawinglai地区8个不同村庄/城镇的50所住宅中进行的。住宅主要是从使用Micro-R测量仪测量的伽马水平和建筑物的建筑类型中选择的。单入口剂量计测得氡和钍的平均浓度分别为75.76 Bq/m3和96.50 Bq/m3,双入口剂量计测得氡和钍的平均浓度分别为63.47 Bq/m3和19.79 Bq/m3。人们发现,就季节数据的趋势和生产质量背后的理论而言,单入口剂量计提供的观测结果比双入口剂量计更可靠。
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引用次数: 0
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