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A proinflammatory stem cell niche drives myelofibrosis through a targetable galectin-1 axis 促炎干细胞龛通过可靶向的galectin-1轴驱动骨髓纤维化。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-09 DOI: 10.1126/scitranslmed.adj7552
Rong Li, Michela Colombo, Guanlin Wang, Antonio Rodriguez-Romera, Camelia Benlabiod, Natalie J. Jooss, Jennifer O’Sullivan, Charlotte K. Brierley, Sally-Ann Clark, Juan M. Pérez Sáez, Pedro Aragón Fernández, Erwin M. Schoof, Bo Porse, Yiran Meng, Abdullah O. Khan, Sean Wen, Pengwei Dong, Wenjiang Zhou, Nikolaos Sousos, Lauren Murphy, Matthew Clarke, Aude-Anais Olijnik, Zoë C. Wong, Christina Simoglou Karali, Korsuk Sirinukunwattana, Hosuk Ryou, Ruggiero Norfo, Qian Cheng, Joana Carrelha, Zemin Ren, Supat Thongjuea, Vijay A. Rathinam, Anandi Krishnan, Daniel Royston, Gabriel A. Rabinovich, Adam J. Mead, Bethan Psaila
Myeloproliferative neoplasms are stem cell–driven cancers associated with a large burden of morbidity and mortality. Most patients present with early-stage disease, but a substantial proportion progress to myelofibrosis or secondary leukemia, advanced cancers with a poor prognosis and high symptom burden. Currently, it remains difficult to predict progression, and therapies that reliably prevent or reverse fibrosis are lacking. A major bottleneck to the discovery of disease-modifying therapies has been an incomplete understanding of the interplay between perturbed cellular and molecular states. Several cell types have individually been implicated, but a comprehensive analysis of myelofibrotic bone marrow is lacking. We therefore mapped the cross-talk between bone marrow cell types in myelofibrotic bone marrow. We found that inflammation and fibrosis are orchestrated by a “quartet” of immune and stromal cell lineages, with basophils and mast cells creating a TNF signaling hub, communicating with megakaryocytes, mesenchymal stromal cells, and proinflammatory fibroblasts. We identified the β-galactoside–binding protein galectin-1 as a biomarker of progression to myelofibrosis and poor survival in multiple patient cohorts and as a promising therapeutic target, with reduced myeloproliferation and fibrosis in vitro and in vivo and improved survival after galectin-1 inhibition. In human bone marrow organoids, TNF increased galectin-1 expression, suggesting a feedback loop wherein the proinflammatory myeloproliferative neoplasm clone creates a self-reinforcing niche, fueling progression to advanced disease. This study provides a resource for studying hematopoietic cell–niche interactions, with relevance for cancer-associated inflammation and disorders of tissue fibrosis.
骨髓增生性肿瘤是干细胞驱动的癌症,发病率和死亡率都很高。大多数患者表现为早期疾病,但相当一部分患者会发展为骨髓纤维化或继发性白血病,这些晚期癌症预后差、症状重。目前,仍难以预测病情的发展,也缺乏能可靠预防或逆转纤维化的疗法。发现疾病改变疗法的一个主要瓶颈是对细胞和分子紊乱状态之间相互作用的不完全了解。有几种细胞类型被单独牵涉其中,但骨髓纤维化骨髓缺乏全面的分析。因此,我们绘制了骨髓纤维化骨髓中骨髓细胞类型之间的交叉对话图。我们发现,炎症和纤维化是由免疫细胞系和基质细胞系的 "四重奏 "协调的,其中嗜碱性粒细胞和肥大细胞创建了一个 TNF 信号枢纽,并与巨核细胞、间充质基质细胞和促炎症成纤维细胞沟通。我们发现β-半乳糖苷结合蛋白galectin-1是骨髓纤维化进展的生物标志物,在多个患者队列中存活率较低,同时也是一个很有前景的治疗靶点,在体外和体内可减少骨髓增殖和纤维化,抑制galectin-1后可提高存活率。在人类骨髓组织细胞中,TNF会增加galectin-1的表达,这表明促炎性骨髓增生性肿瘤克隆会形成一个自我强化的龛位,助长疾病向晚期发展的反馈回路。这项研究为研究造血细胞-生态位相互作用提供了资源,与癌症相关的炎症和组织纤维化疾病有关。
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引用次数: 0
Sleep-sensitive dopamine receptor expression in male mice underlies attention deficits after a critical period of early adversity 雄性小鼠对睡眠敏感的多巴胺受体表达是早期逆境关键期后注意力缺陷的基础。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-09 DOI: 10.1126/scitranslmed.adh9763
Yuichi Makino, Nathaniel W. Hodgson, Emma Doenier, Anna Victoria Serbin, Koya Osada, Pietro Artoni, Matthew Dickey, Breanna Sullivan, Amelia Potter-Dickey, Jelena Komanchuk, Bikram Sekhon, Nicole Letourneau, Neal D. Ryan, Jeanette Trauth, Judy L. Cameron, Takao K. Hensch
Early life stress (ELS) yields cognitive impairments of unknown molecular and physiological origin. We found that fragmented maternal care of mice during a neonatal critical period from postnatal days P2–9 elevated dopamine receptor D2R and suppressed D4R expression, specifically within the anterior cingulate cortex (ACC) in only the male offspring. This was associated with poor performance on a two-choice visual attention task, which was acutely rescued in adulthood by local or systemic pharmacological rebalancing of D2R/D4R activity. Furthermore, ELS male mice demonstrated heightened hypothalamic orexin and persistently disrupted sleep. Given that acute sleep deprivation in normally reared male mice mimicked the ACC dopamine receptor subtype modulation and disrupted attention of ELS mice, sleep loss likely underlies cognitive deficits in ELS mice. Likewise, sleep impairment mediated the attention deficits associated with early adversity in human children, as demonstrated by path analysis on data collected with multiple questionnaires for a large child cohort. A deeper understanding of the sex-specific cognitive consequences of ELS thus has the potential to reveal therapeutic strategies for overcoming them.
早期生活压力(ELS)会导致认知障碍,其分子和生理原因尚不清楚。我们发现,在小鼠出生后第 2-9 天的关键时期,母体对小鼠的零散照顾会升高多巴胺受体 D2R 并抑制 D4R 的表达,特别是在雄性后代的前扣带回皮层(ACC)中。这与视觉注意力二选一任务的表现不佳有关,而在成年后,通过局部或全身药物重新平衡D2R/D4R活性可迅速缓解这种情况。此外,ELS 雄性小鼠表现出下丘脑奥曲肽增加和睡眠持续中断。鉴于在正常饲养的雄性小鼠中进行急性睡眠剥夺可模拟 ELS 小鼠的 ACC 多巴胺受体亚型调节和注意力紊乱,因此睡眠不足可能是 ELS 小鼠认知缺陷的原因。同样,通过对一个大型儿童队列的多种问卷数据进行路径分析,也证明了睡眠障碍介导了与人类儿童早期逆境相关的注意力缺陷。因此,深入了解ELS的性别特异性认知后果有可能揭示克服这些后果的治疗策略。
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引用次数: 0
A multispecific antibody against SARS-CoV-2 prevents immune escape in vitro and confers prophylactic protection in vivo 针对 SARS-CoV-2 的多特异性抗体可防止体外免疫逃逸,并在体内提供预防性保护。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-09 DOI: 10.1126/scitranslmed.ado9026
John Misasi, Ronnie R. Wei, Lingshu Wang, Amarendra Pegu, Chih-Jen Wei, Olamide K. Oloniniyi, Tongqing Zhou, Juan I. Moliva, Bingchun Zhao, Misook Choe, Eun Sung Yang, Yi Zhang, Marika Boruszczak, Man Chen, Kwanyee Leung, Juan Li, Zhi-Yong Yang, Hanne Andersen, Kevin Carlton, Sucheta Godbole, Darcy R. Harris, Amy R. Henry, Vera B. Ivleva, Q. Paula Lei, Cuiping Liu, Lindsay Longobardi, Jonah S. Merriam, Danielle Nase, Adam S. Olia, Laurent Pessaint, Maciel Porto, Wei Shi, Shannon M. Wallace, Jeremy J. Wolff, Daniel C. Douek, Mehul S. Suthar, Jason G. Gall, Richard A. Koup, Peter D. Kwong, John R. Mascola, Gary J. Nabel, Nancy J. Sullivan
Despite effective countermeasures, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) persists worldwide because of its ability to diversify and evade human immunity. This evasion stems from amino acid substitutions, particularly in the receptor binding domain (RBD) of the spike protein that confers resistance to vaccine-induced antibodies and antibody therapeutics. To constrain viral escape through resistance mutations, we combined antibody variable regions that recognize different RBD sites into multispecific antibodies. Here, we describe multispecific antibodies, including a trivalent trispecific antibody that potently neutralized diverse SARS-CoV-2 variants and prevented virus escape more effectively than single antibodies or mixtures of the parental antibodies. Despite being generated before the appearance of Omicron, this trispecific antibody neutralized all major Omicron variants through BA.4/BA.5 at nanomolar concentrations. Negative stain electron microscopy suggested that synergistic neutralization was achieved by engaging different epitopes in specific orientations that facilitated binding across more than one spike protein. Moreover, a tetravalent trispecific antibody containing the same variable regions as the trivalent trispecific antibody also protected Syrian hamsters against Omicron variants BA.1, BA.2, and BA.5 challenge, each of which uses different amino acid substitutions to mediate escape from therapeutic antibodies. These results demonstrated that multispecific antibodies have the potential to provide broad SARS-CoV-2 coverage, decrease the likelihood of escape, simplify treatment, and provide a strategy for antibody therapies that could help eliminate pandemic spread for this and other pathogens.
尽管采取了有效的应对措施,但严重急性呼吸系统综合症冠状病毒 2(SARS-CoV-2)仍在全球范围内持续存在,因为它具有多样化和逃避人类免疫的能力。这种逃避能力源于氨基酸的置换,尤其是尖峰蛋白的受体结合域(RBD),该受体结合域对疫苗诱导的抗体和抗体疗法具有抗性。为了限制病毒通过抗性突变逃逸,我们将识别不同 RBD 位点的抗体可变区组合成多特异性抗体。在这里,我们描述了多特异性抗体,包括一种三价三特异性抗体,它能有效中和多种 SARS-CoV-2 变体,并比单一抗体或亲代抗体的混合物更有效地防止病毒逃逸。尽管这种三特异性抗体是在 Omicron 出现之前产生的,但在纳摩尔浓度下,它能中和从 BA.4/BA.5 到 Omicron 的所有主要变体。负染色电子显微镜表明,协同中和是通过不同表位的特异取向结合实现的,这种结合有利于跨越多个尖峰蛋白。此外,含有与三价三特异性抗体相同可变区的四价三特异性抗体也能保护叙利亚仓鼠免受奥米克龙变体 BA.1、BA.2 和 BA.5 的挑战,每种变体都使用不同的氨基酸取代来介导治疗性抗体的逃避。这些结果表明,多特异性抗体有可能提供广泛的SARS-CoV-2覆盖范围,降低逃逸的可能性,简化治疗,并为抗体疗法提供一种策略,有助于消除这种病原体和其他病原体的大流行。
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引用次数: 0
An altered natural killer cell immunophenotype characterizes clinically severe pediatric RSV infection 自然杀伤细胞免疫表型的改变是临床上严重的小儿 RSV 感染的特征。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-09 DOI: 10.1126/scitranslmed.ado6606
Roisin B. Reilly, Saïsha K. Ramdour, Mary E. Fuhlbrigge, Luciana P. Tavares, Steven J. Staffa, Jocelyn M. Booth, Nandini Krishnamoorthy, Bruce D. Levy, Melody G. Duvall
Respiratory syncytial virus (RSV) infects nearly all children by 2 years of age and is a leading cause of pediatric hospitalizations. A subset of children with RSV infection (RSV+ children) develop respiratory failure requiring intensive care, but immune mechanisms distinguishing severe pediatric RSV infection are not fully elucidated. Natural killer (NK) cells are key innate immune effectors of viral host defense. In this study of 47 critically ill RSV+ children, we coupled NK cell immunophenotype and cytotoxic function with clinical parameters to identify an NK cell immune signature of severe pediatric RSV disease. Airway NK cells were increased in intubated RSV+ children with severe hypoxemia and prolonged duration of mechanical ventilation and were correlated with clinical severity scores. Peripheral blood NK cells were decreased in RSV+ patients and had altered activating receptor expression, with increased expression of CD69 and decreased expression of NKG2D. Ex vivo, circulating NK cells from RSV+ patients exhibited functional impairment characterized by decreased cytotoxicity as well as aberrant immune synapse assembly and lytic granule trafficking. NK cell frequency and phenotype correlated with clinical measures that defined disease severity. These findings implicate a role for NK cells in mediating RSV immunopathology and suggest that an altered NK cell immunophenotype is associated with severe RSV disease in young children.
几乎所有两岁前的儿童都会感染呼吸道合胞病毒(RSV),这是导致儿科住院治疗的主要原因。感染 RSV 的儿童(RSV+ 儿童)中有一部分会出现呼吸衰竭,需要重症监护,但区分严重儿科 RSV 感染的免疫机制尚未完全阐明。自然杀伤(NK)细胞是病毒宿主防御的关键先天免疫效应器。在这项针对 47 名 RSV+ 重症患儿的研究中,我们将 NK 细胞免疫表型和细胞毒性功能与临床参数相结合,确定了重症儿科 RSV 疾病的 NK 细胞免疫特征。在插管的 RSV+ 患儿中,气道 NK 细胞在严重低氧血症和长时间机械通气的患儿中增加,并与临床严重程度评分相关。RSV+患者的外周血NK细胞减少,活化受体表达发生改变,CD69表达增加,NKG2D表达减少。在体内外,RSV+ 患者的循环 NK 细胞表现出功能损伤,其特点是细胞毒性下降以及免疫突触组装和溶解颗粒贩运异常。NK 细胞的频率和表型与定义疾病严重程度的临床指标相关。这些发现暗示了 NK 细胞在介导 RSV 免疫病理中的作用,并表明 NK 细胞免疫表型的改变与幼儿严重的 RSV 疾病有关。
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引用次数: 0
Glucocorticoids induce a maladaptive epithelial stress response to aggravate acute kidney injury 糖皮质激素诱导适应不良的上皮应激反应,加剧急性肾损伤
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-02 DOI: 10.1126/scitranslmed.adk5005
Luping Zhou, Marc Torres Pereiro, Yanqun Li, Marcus Derigs, Carsten Kuenne, Thomas Hielscher, Wei Huang, Bettina Kränzlin, Gang Tian, Kazuhiro Kobayashi, Gia-Hue Natalie Lu, Kevin Roedl, Claudia Schmidt, Stefan Günther, Mario Looso, Johannes Huber, Yong Xu, Thorsten Wiech, Jan-Peter Sperhake, Dominic Wichmann, Hermann-Josef Gröne, Thomas Worzfeld
Acute kidney injury (AKI) is a frequent and challenging clinical condition associated with high morbidity and mortality and represents a common complication in critically ill patients with COVID-19. In AKI, renal tubular epithelial cells (TECs) are a primary site of damage, and recovery from AKI depends on TEC plasticity. However, the molecular mechanisms underlying adaptation and maladaptation of TECs in AKI remain largely unclear. Here, our study of an autopsy cohort of patients with COVID-19 provided evidence that injury of TECs by myoglobin, released as a consequence of rhabdomyolysis, is a major pathophysiological mechanism for AKI in severe COVID-19. Analyses of human kidney biopsies, mouse models of myoglobinuric and gentamicin-induced AKI, and mouse kidney tubuloids showed that TEC injury resulted in activation of the glucocorticoid receptor by endogenous glucocorticoids, which aggravated tubular damage. The detrimental effect of endogenous glucocorticoids on injured TECs was exacerbated by the administration of a widely clinically used synthetic glucocorticoid, dexamethasone, as indicated by experiments in mouse models of myoglobinuric- and folic acid–induced AKI, human and mouse kidney tubuloids, and human kidney slice cultures. Mechanistically, studies in mouse models of AKI, mouse tubuloids, and human kidney slice cultures demonstrated that glucocorticoid receptor signaling in injured TECs orchestrated a maladaptive transcriptional program to hinder DNA repair, amplify injury-induced DNA double-strand break formation, and dampen mTOR activity and mitochondrial bioenergetics. This study identifies glucocorticoid receptor activation as a mechanism of epithelial maladaptation, which is functionally important for AKI.
急性肾损伤(AKI)是一种常见且具有挑战性的临床症状,与高发病率和高死亡率相关,是COVID-19重症患者的常见并发症。在 AKI 中,肾小管上皮细胞(TEC)是主要的损伤部位,而 AKI 的恢复取决于 TEC 的可塑性。然而,AKI 中肾小管上皮细胞适应和不适应的分子机制在很大程度上仍不清楚。在此,我们对 COVID-19 患者尸检队列的研究提供了证据,证明横纹肌溶解释放的肌红蛋白对 TEC 的损伤是严重 COVID-19 患者发生 AKI 的主要病理生理机制。对人类肾活检组织、肌红蛋白尿和庆大霉素诱导的 AKI 小鼠模型以及小鼠肾小管的分析表明,TEC 损伤导致内源性糖皮质激素激活糖皮质激素受体,从而加重了肾小管损伤。在肌红蛋白尿和叶酸诱导的 AKI 小鼠模型、人和小鼠肾小管以及人肾切片培养物中进行的实验表明,给予临床广泛使用的合成糖皮质激素地塞米松会加剧内源性糖皮质激素对损伤的 TEC 的有害作用。从机理上讲,对小鼠 AKI 模型、小鼠肾小管和人肾切片培养物的研究表明,损伤的 TEC 中糖皮质激素受体信号传导协调了一种不适应的转录程序,阻碍了 DNA 修复,扩大了损伤诱导的 DNA 双链断裂的形成,抑制了 mTOR 活性和线粒体生物能。这项研究确定了糖皮质激素受体激活是上皮适应不良的一种机制,而上皮适应不良在功能上对 AKI 非常重要。
{"title":"Glucocorticoids induce a maladaptive epithelial stress response to aggravate acute kidney injury","authors":"Luping Zhou,&nbsp;Marc Torres Pereiro,&nbsp;Yanqun Li,&nbsp;Marcus Derigs,&nbsp;Carsten Kuenne,&nbsp;Thomas Hielscher,&nbsp;Wei Huang,&nbsp;Bettina Kränzlin,&nbsp;Gang Tian,&nbsp;Kazuhiro Kobayashi,&nbsp;Gia-Hue Natalie Lu,&nbsp;Kevin Roedl,&nbsp;Claudia Schmidt,&nbsp;Stefan Günther,&nbsp;Mario Looso,&nbsp;Johannes Huber,&nbsp;Yong Xu,&nbsp;Thorsten Wiech,&nbsp;Jan-Peter Sperhake,&nbsp;Dominic Wichmann,&nbsp;Hermann-Josef Gröne,&nbsp;Thomas Worzfeld","doi":"10.1126/scitranslmed.adk5005","DOIUrl":"10.1126/scitranslmed.adk5005","url":null,"abstract":"<div >Acute kidney injury (AKI) is a frequent and challenging clinical condition associated with high morbidity and mortality and represents a common complication in critically ill patients with COVID-19. In AKI, renal tubular epithelial cells (TECs) are a primary site of damage, and recovery from AKI depends on TEC plasticity. However, the molecular mechanisms underlying adaptation and maladaptation of TECs in AKI remain largely unclear. Here, our study of an autopsy cohort of patients with COVID-19 provided evidence that injury of TECs by myoglobin, released as a consequence of rhabdomyolysis, is a major pathophysiological mechanism for AKI in severe COVID-19. Analyses of human kidney biopsies, mouse models of myoglobinuric and gentamicin-induced AKI, and mouse kidney tubuloids showed that TEC injury resulted in activation of the glucocorticoid receptor by endogenous glucocorticoids, which aggravated tubular damage. The detrimental effect of endogenous glucocorticoids on injured TECs was exacerbated by the administration of a widely clinically used synthetic glucocorticoid, dexamethasone, as indicated by experiments in mouse models of myoglobinuric- and folic acid–induced AKI, human and mouse kidney tubuloids, and human kidney slice cultures. Mechanistically, studies in mouse models of AKI, mouse tubuloids, and human kidney slice cultures demonstrated that glucocorticoid receptor signaling in injured TECs orchestrated a maladaptive transcriptional program to hinder DNA repair, amplify injury-induced DNA double-strand break formation, and dampen mTOR activity and mitochondrial bioenergetics. This study identifies glucocorticoid receptor activation as a mechanism of epithelial maladaptation, which is functionally important for AKI.</div>","PeriodicalId":21580,"journal":{"name":"Science Translational Medicine","volume":"16 767","pages":""},"PeriodicalIF":15.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A peptide encoded by upstream open reading frame of MYC binds to tropomyosin receptor kinase B and promotes glioblastoma growth in mice 由 MYC 上游开放阅读框编码的肽与肌球蛋白受体激酶 B 结合并促进小鼠胶质母细胞瘤的生长
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-02 DOI: 10.1126/scitranslmed.adk9524
Fanying Li, Kailin Yang, Xinya Gao, Maolei Zhang, Danling Gu, Xujia Wu, Chenfei Lu, Qiulian Wu, Deobrat Dixit, Ryan C. Gimple, Yongping You, Stephen C. Mack, Yu Shi, Tiebang Kang, Sameer A. Agnihotri, Michael D. Taylor, Jeremy N. Rich, Nu Zhang, Xiuxing Wang
MYC promotes tumor growth through multiple mechanisms. Here, we show that, in human glioblastomas, the variant MYC transcript encodes a 114–amino acid peptide, MYC pre-mRNA encoded protein (MPEP), from the upstream open reading frame (uORF) MPEP. Secreted MPEP promotes patient-derived xenograft tumor growth in vivo, independent of MYC through direct binding, and activation of tropomyosin receptor kinase B (TRKB), which induces downstream AKT-mTOR signaling. Targeting MPEP through genetic ablation reduced growth of patient-derived 4121 and 3691 glioblastoma stem cells. Administration of an MPEP-neutralizing antibody in combination with a small-molecule TRKB inhibitor reduced glioblastoma growth in patient-derived xenograft tumor–bearing mice. The overexpression of MPEP in surgical glioblastoma specimens predicted a poor prognosis, supporting its clinical relevance. In summary, our results demonstrate that tumor-specific translation of a MYC-associated uORF promotes glioblastoma growth, suggesting a new therapeutic strategy for glioblastoma.
MYC 通过多种机制促进肿瘤生长。在这里,我们发现,在人类胶质母细胞瘤中,变异的 MYC 转录本从上游开放阅读框(uORF)MPEP 编码一种 114 氨基酸的肽,即 MYC 前 mRNA 编码蛋白(MPEP)。分泌的 MPEP 通过直接结合和激活肌球蛋白受体激酶 B (TRKB),诱导下游 AKT-mTOR 信号传导,促进患者体内异种移植肿瘤的生长,而不依赖于 MYC。通过基因消融来靶向MPEP,可减少源自患者的4121和3691胶质母细胞瘤干细胞的生长。将MPEP中和抗体与小分子TRKB抑制剂联合使用,可减少胶质母细胞瘤在患者来源异种移植肿瘤小鼠体内的生长。手术胶质母细胞瘤标本中 MPEP 的过表达预示着不良预后,这支持了 MPEP 的临床意义。总之,我们的研究结果表明,MYC相关uORF的肿瘤特异性翻译会促进胶质母细胞瘤的生长,这为胶质母细胞瘤的治疗提供了一种新策略。
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引用次数: 0
Dynamic structural remodeling of LINC01956 enhances temozolomide resistance in MGMT-methylated glioblastoma LINC01956 的动态结构重塑增强了 MGMT 甲基化胶质母细胞瘤对替莫唑胺的耐药性
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-02 DOI: 10.1126/scitranslmed.ado1573
Xinyi Liao, Shuxia Zhang, Xincheng Li, Wanying Qian, Man Li, Suwen Chen, Xingui Wu, Xuexin Yu, Ziwen Li, Miaoling Tang, Yingru Xu, Ruyuan Yu, Qiliang Zhang, Geyan Wu, Nu Zhang, Libing Song, Jun Li
The mechanisms underlying stimuli-induced dynamic structural remodeling of RNAs for the maintenance of cellular physiological function and survival remain unclear. Here, we showed that in MGMT promoter–methylated glioblastoma (GBM), the RNA helicase DEAD-box helicase 46 (DDX46) is phosphorylated by temozolomide (TMZ)–activated checkpoint kinase 1 (CHK1), resulting in a dense-to-loose conformational change and an increase in DDX46 helicase activity. DDX46-mediated tertiary structural remodeling of LINC01956 exposes the binding motifs of LINC01956 to the 3′ untranslated region of O6-methylguanine DNA methyltransferase (MGMT). This accelerates recruitment of MGMT mRNA to the RNA export machinery and transportation of MGMT mRNA from the nucleus to the cytoplasm, leading to increased MGMT abundance and TMZ resistance. Using patient-derived xenograft (PDX) and tumor organoid models, we found that treatment with the CHK1 inhibitor SRA737abolishes TMZ-induced structural remodeling of LINC01956 and subsequent MGMT up-regulation, resensitizing TMZ-resistant MGMT promoter–methylated GBM to TMZ. In conclusion, these findings highlight a mechanism underlying temozolomide-induced RNA structural remodeling and may represent a potential therapeutic strategy for patients with TMZ-resistant MGMT promoter–methylated GBM.
刺激诱导 RNA 动态结构重塑以维持细胞生理功能和存活的机制仍不清楚。在这里,我们发现在MGMT启动子甲基化的胶质母细胞瘤(GBM)中,RNA螺旋酶DEAD-box helicase 46(DDX46)被替莫唑胺(TMZ)激活的检查点激酶1(CHK1)磷酸化,导致致密到松弛的构象变化以及DDX46螺旋酶活性的增加。DDX46 介导的 LINC01956 三级结构重塑使 LINC01956 与 O 6 -甲基鸟嘌呤 DNA 甲基转移酶(MGMT)3′非翻译区的结合基序暴露出来。这加速了 MGMT mRNA 与 RNA 导出机制的结合,并将 MGMT mRNA 从细胞核运输到细胞质,从而导致 MGMT 丰度增加和 TMZ 抗性。利用患者衍生异种移植(PDX)和肿瘤类器官模型,我们发现用 CHK1 抑制剂 SRA737 治疗可消除 TMZ 诱导的 LINC01956 结构重塑和随后的 MGMT 上调,使 TMZ 耐药的 MGMT 启动子甲基化 GBM 对 TMZ 再敏感。总之,这些发现突显了替莫唑胺诱导 RNA 结构重塑的机制,可能是治疗 TMZ 耐药 MGMT 启动子甲基化 GBM 患者的一种潜在策略。
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引用次数: 0
LRRK2 regulates production of reactive oxygen species in cell and animal models of Parkinson’s disease LRRK2 在帕金森病细胞和动物模型中调节活性氧的产生
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-02 DOI: 10.1126/scitranslmed.adl3438
Matthew T. Keeney, Emily M. Rocha, Eric K. Hoffman, Kyle Farmer, Roberto Di Maio, Julie Weir, Weston G. Wagner, Xiaoping Hu, Courtney L. Clark, Sandra L. Castro, Abigail Scheirer, Marco Fazzari, Briana R. De Miranda, Sean A. Pintchovski, William D. Shrader, Patrick J. Pagano, Teresa G. Hastings, J. Timothy Greenamyre
Oxidative stress has long been implicated in Parkinson’s disease (PD) pathogenesis, although the sources and regulation of reactive oxygen species (ROS) production are poorly defined. Pathogenic mutations in the gene encoding leucine-rich repeat kinase 2 (LRRK2) are associated with increased kinase activity and a greater risk of PD. The substrates and downstream consequences of elevated LRRK2 kinase activity are still being elucidated, but overexpression of mutant LRRK2 has been associated with oxidative stress, and antioxidants reportedly mitigate LRRK2 toxicity. Here, using CRISPR-Cas9 gene-edited HEK293 cells, RAW264.7 macrophages, rat primary ventral midbrain cultures, and PD patient–derived lymphoblastoid cells, we found that elevated LRRK2 kinase activity was associated with increased ROS production and lipid peroxidation and that this was blocked by inhibitors of either LRRK2 kinase or NADPH oxidase 2 (NOX2). Oxidative stress induced by the pesticide rotenone was ameliorated by LRRK2 kinase inhibition and was absent in cells devoid of LRRK2. In a rat model of PD induced by rotenone, a LRRK2 kinase inhibitor prevented the lipid peroxidation and NOX2 activation normally seen in nigral dopaminergic neurons in this model. Mechanistically, LRRK2 kinase activity was shown to regulate phosphorylation of serine-345 in the p47phox subunit of NOX2. This, in turn, led to translocation of p47phox from the cytosol to the membrane-associated gp91phox (NOX2) subunit, activation of the NOX2 enzyme complex, and production of ROS. Thus, LRRK2 kinase activity may drive cellular ROS production in PD through the regulation of NOX2 activity.
氧化应激长期以来一直与帕金森病(PD)的发病机制有关,但活性氧(ROS)产生的来源和调控机制尚不明确。富亮氨酸重复激酶 2(LRRK2)编码基因的致病突变与激酶活性增加和帕金森病发病风险增高有关。LRRK2激酶活性升高的底物和下游后果仍在阐明之中,但突变型LRRK2的过表达与氧化应激有关,据报道抗氧化剂可减轻LRRK2的毒性。在这里,我们使用 CRISPR-Cas9 基因编辑的 HEK293 细胞、RAW264.7 巨噬细胞、大鼠原代腹侧中脑培养物和帕金森病患者衍生的淋巴母细胞,发现 LRRK2 激酶活性的升高与 ROS 生成和脂质过氧化的增加有关,而 LRRK2 激酶或 NADPH 氧化酶 2 (NOX2) 的抑制剂可阻断 ROS 生成和脂质过氧化的增加。农药鱼藤酮诱导的氧化应激可通过抑制 LRRK2 激酶而得到改善,而在没有 LRRK2 的细胞中则没有这种应激。在由鱼藤酮诱导的老年痴呆症大鼠模型中,LRRK2激酶抑制剂阻止了该模型中黑质多巴胺能神经元通常出现的脂质过氧化和NOX2激活。从机理上讲,LRRK2 激酶活性可调节 NOX2 的 p47 phox 亚基中丝氨酸-345 的磷酸化。这反过来又导致 p47 phox 从细胞质转位到与膜结合的 gp91 phox(NOX2)亚基,激活 NOX2 酶复合物并产生 ROS。因此,LRRK2激酶的活性可能通过调节NOX2的活性来驱动帕金森病细胞ROS的产生。
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引用次数: 0
A microbiome-directed therapeutic food for children recovering from severe acute malnutrition 针对严重急性营养不良恢复期儿童的微生物导向食疗食品
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-10-02 DOI: 10.1126/scitranslmed.adn2366
Steven J. Hartman, Matthew C. Hibberd, Ishita Mostafa, Nurun N. Naila, Md. Munirul Islam, Mahabub Uz Zaman, Sayeeda Huq, Mustafa Mahfuz, Md. Tazul Islam, Kallol Mukherji, Vaha Akbary Moghaddam, Robert Y. Chen, Michael A. Province, Daniel M. Webber, Suzanne Henrissat, Bernard Henrissat, Nicolas Terrapon, Dmitry A. Rodionov, Andrei L. Osterman, Michael J. Barratt, Tahmeed Ahmed, Jeffrey I. Gordon
Globally, severe acute malnutrition (SAM), defined as a weight-for-length z-score more than three SDs below a reference mean (WLZ < −3), affects 14 million children under 5 years of age. Complete anthropometric recovery after standard, short-term interventions is rare, with children often left with moderate acute malnutrition (MAM; WLZ −2 to −3). We conducted a randomized controlled trial (RCT) involving 12- to 18-month-old Bangladeshi children from urban and rural sites, who, after initial hospital-based treatment for SAM, received a 3-month intervention with a microbiome-directed complementary food (MDCF-2) or a calorically more dense, standard ready-to-use supplementary food (RUSF). The rate of WLZ improvement was significantly greater in MDCF-2–treated children (P = 8.73 × 10−3), similar to our previous RCT of Bangladeshi children with MAM without antecedent SAM (P = 0.032). A correlated meta-analysis of plasma levels of 4520 proteins in both RCTs revealed 215 positively associated with WLZ (largely representing musculoskeletal and central nervous system development) and 44 negatively associated (primarily related to immune activation). Moreover, the positively associated proteins were significantly enriched by MDCF-2 (q = 1.1 × 10−6). Characterizing the abundances of 754 bacterial metagenome-assembled genomes in serially collected fecal samples disclosed the effects of acute rehabilitation for SAM on the microbiome and how, during treatment for MAM, specific strains of Prevotella copri function at the intersection between MDCF-2 glycan metabolism and anthropometric recovery. These results provide a rationale for further testing the generalizability of MDCF efficacy and for identifying biomarkers to define treatment responses.
全球有 1400 万 5 岁以下儿童患有严重急性营养不良(SAM),其定义是体重身长 z 值比参考平均值低三个标准差以上(WLZ < -3)。经过标准的短期干预后,人体测量完全恢复的情况非常罕见,儿童通常会遗留中度急性营养不良(MAM;WLZ -2至-3)。我们进行了一项随机对照试验(RCT),研究对象是来自城市和农村地区的 12 至 18 个月大的孟加拉国儿童,这些儿童在接受了医院对中度急性营养不良的初步治疗后,接受了为期 3 个月的微生物指导型辅食(MDCF-2)或热量更高的标准即食辅食(RUSF)干预。接受MDCF-2治疗的儿童的WLZ改善率明显更高(P = 8.73 × 10 -3),这与我们之前对患有MAM但无先兆SAM的孟加拉国儿童进行的研究结果相似(P = 0.032)。对两项研究中 4520 种蛋白质的血浆水平进行的相关荟萃分析表明,215 种蛋白质与 WLZ 呈正相关(主要代表肌肉骨骼和中枢神经系统的发育),44 种呈负相关(主要与免疫激活有关)。此外,MDCF-2 显著富集了正相关蛋白质(q = 1.1 × 10 -6)。对连续收集的粪便样本中 754 个细菌元基因组的丰度进行鉴定,揭示了急性 SAM 康复对微生物组的影响,以及在 MAM 治疗期间,特定的 copri Prevotella 菌株如何在 MDCF-2 糖代谢和人体测量恢复之间发挥作用。这些结果为进一步测试 MDCF 疗效的普遍性和确定生物标志物以确定治疗反应提供了理论依据。
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引用次数: 0
Neutrophil extracellular traps protect the kidney from ascending infection and are required for a positive leukocyte dipstick test 中性粒细胞胞外捕获器可保护肾脏免受上升型感染,是白细胞量筒检测呈阳性的必要条件
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-25 DOI: 10.1126/scitranslmed.adh5090
Andrew P. Stewart, Kevin W. Loudon, Matthew Routledge, Colin Y. C. Lee, Patrick Trotter, Nathan Richoz, Eleanor Gillman, Robin Antrobus, James Mccaffrey, David Posner, Andrew Conway Morris, Fiona E. Karet Frankl, Menna R. Clatworthy
Lower urinary tract infection (UTI) is common but only rarely complicated by pyelonephritis. However, the mechanisms preventing extension to the kidney are unclear. Here, we identified neutrophil extracellular traps (NETs) in healthy human urine that provide an antibacterial defense strategy within the urinary tract. In both in vivo murine models of UTI where uropathogenic E. coli are inoculated into the bladder and ex vivo human urine models, NETs interacted with uromodulin to form large webs that entrapped the bacteria. Peptidyl arginine deiminase 4 (PADI4) inhibition in mice blocked NETosis and resulted in progression of cystitis into pyelonephritis, suggesting that NETosis of urinary neutrophils acts to prevent bacterial ascent into the kidney. Analysis of UK Biobank data revealed that genetic variants in PADI4 that associated with increased risk of rheumatoid arthritis in multiple genome-wide association studies were consistently associated with reduced susceptibility to UTI. Last, we showed that urine dipstick testing for leukocyte esterase was negative in the presence of intact blood neutrophils but became positive when neutrophils were stimulated to NET, and this could be prevented by selective PADI4 inhibition, demonstrating that this test does not detect absolute neutrophil count, as has long been assumed, but specifically detects neutrophils that have undergone NETosis. These findings highlight the role of NETosis in preventing ascending infections in the urinary tract and improve our understanding of one of the most common clinical tests in medicine.
下尿路感染(UTI)很常见,但很少并发肾盂肾炎。然而,防止感染扩展到肾脏的机制尚不清楚。在这里,我们在健康人的尿液中发现了中性粒细胞胞外捕获物(NET),它们在尿路中提供了一种抗菌防御策略。在将尿路致病性大肠杆菌接种到膀胱的体内小鼠UTI模型和体外人体尿液模型中,NETs与尿液调节蛋白相互作用,形成大网将细菌困住。在小鼠体内抑制肽基精氨酸脱氨酶 4 (PADI4)可阻断 NETosis,并导致膀胱炎发展为肾盂肾炎,这表明尿液中性粒细胞的 NETosis 起到了防止细菌进入肾脏的作用。对英国生物库数据的分析表明,在多项全基因组关联研究中,与类风湿性关节炎风险增加有关的 PADI4 基因变异与UTI 易感性的降低一直存在关联。最后,我们还发现,尿液浸量计检测白细胞酯酶在有完整的血液中性粒细胞存在的情况下呈阴性,但当中性粒细胞受刺激发生NET时则呈阳性,而这可以通过选择性抑制PADI4来防止,这表明该检测并不像人们长期以来认为的那样检测绝对的中性粒细胞数量,而是专门检测发生NETosis的中性粒细胞。这些发现强调了NETosis在预防泌尿道升天性感染中的作用,并加深了我们对医学中最常见的临床检验之一的理解。
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