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A deep intronic splice–altering AIRE variant causes APECED syndrome through antisense oligonucleotide-targetable pseudoexon inclusion 深内含子剪接改变的AIRE变体通过反义寡核苷酸靶向伪外显子包涵导致APECED综合征
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-18 DOI: 10.1126/scitranslmed.adk0845
Sebastian Ochoa, Amy P. Hsu, Andrew J. Oler, Dhaneshwar Kumar, Daniel Chauss, Jan Piet van Hamburg, Gustaaf G. van Laar, Vasileios Oikonomou, Sundar Ganesan, Elise M. N. Ferré, Monica M. Schmitt, Tom DiMaggio, Princess Barber, Gregory M. Constantine, Lindsey B. Rosen, Paul G. Auwaerter, Bhumika Gandhi, Jennifer L. Miller, Rachel Eisenberg, Arye Rubinstein, Edith Schussler, Erjola Balliu, Vandana Shashi, Olaf Neth, Peter Olbrich, Kim My Le, Nanni Mamia, Saila Laakso, Pasi I. Nevalainen, Juha Grönholm, Mikko R. J. Seppänen, Louis Boon, Gulbu Uzel, Luis M. Franco, Theo Heller, Karen K. Winer, Rajarshi Ghosh, Bryce A. Seifert, Magdalena Walkiewicz, Luigi D. Notarangelo, Qing Zhou, Ivona Askentijevich, William Gahl, Cliffton L. Dalgard, Lalith Perera, Behdad Afzali, Sander W. Tas, Steven M. Holland, Michail S. Lionakis
Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a life-threatening monogenic autoimmune disorder primarily caused by biallelic deleterious variants in the autoimmune regulator (AIRE) gene. We prospectively evaluated 104 patients with clinically diagnosed APECED syndrome and identified 17 patients (16%) from 14 kindreds lacking biallelic AIRE variants in exons or flanking intronic regions; 15 had Puerto Rican ancestry. Through whole-genome sequencing, we identified a deep intronic AIRE variant (c.1504-818 G>A) cosegregating with the disease in all 17 patients. We developed a culture system of AIRE-expressing primary patient monocyte-derived dendric cells and demonstrated that c.1504-818 G>A creates a cryptic splice site and activates inclusion of a 109–base pair frame-shifting pseudoexon. We also found low-level AIRE expression in patient-derived lymphoblastoid cell lines (LCLs) and confirmed pseudoexon inclusion in independent extrathymic AIRE–expressing cell lines. Through protein modeling and transcriptomic analyses of AIRE-transfected human embryonic kidney 293 and thymic epithelial cell 4D6 cells, we showed that this variant alters the carboxyl terminus of the AIRE protein, abrogating its function. Last, we developed an antisense oligonucleotide (ASO) that reversed pseudoexon inclusion and restored the normal AIRE transcript sequence in LCLs. Thus, our findings revealed c.1504-818 G>A as a founder APECED-causing AIRE variant in the Puerto Rican population and uncovered pseudoexon inclusion as an ASO-reversible genetic mechanism underlying APECED.
自身免疫性多内分泌病-念珠菌病-外胚层营养不良症(APECED)是一种危及生命的单基因自身免疫性疾病,主要由自身免疫调节剂(AIRE)基因中的双倍性有害变体引起。我们对 104 名临床诊断为 APECED 综合征的患者进行了前瞻性评估,并从 14 个在外显子或侧翼内含子区缺乏双倍性 AIRE 变异的家族中发现了 17 名患者(16%);其中 15 名患者有波多黎各血统。通过全基因组测序,我们在所有 17 名患者中发现了一个与疾病共存的 AIRE 深度内含子变异(c.1504-818 G>A)。我们建立了一个表达 AIRE 的原发性患者单核细胞衍生树突细胞培养系统,并证明 c.1504-818 G>A 创建了一个隐性剪接位点,并激活了 109 碱基对移帧假外显子的包含。我们还在患者衍生的淋巴母细胞系(LCLs)中发现了低水平的 AIRE 表达,并在独立的外胚层 AIRE 表达细胞系中证实了假外显子的包含。通过对转染 AIRE 的人胚肾 293 细胞和胸腺上皮细胞 4D6 细胞进行蛋白质建模和转录组分析,我们发现该变体改变了 AIRE 蛋白的羧基端,从而削弱了其功能。最后,我们开发了一种反义寡核苷酸(ASO),它能逆转假外显子的包含,并恢复 LCLs 中 AIRE 转录本的正常序列。因此,我们的研究结果显示,c.1504-818 G>A 是波多黎各人群中导致 APECED 的 AIRE 基因变异,并揭示了假外显子包涵是一种 ASO 可逆的 APECED 遗传机制。
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引用次数: 0
Perfusion imaging metrics after acute traumatic spinal cord injury are associated with injury severity in rats and humans 大鼠和人类急性创伤性脊髓损伤后的灌注成像指标与损伤严重程度有关
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-18 DOI: 10.1126/scitranslmed.adn4970
Zin Z. Khaing, Jannik Leyendecker, Jennifer N. Harmon, Sananthan Sivakanthan, Lindsay N. Cates, Jeffrey E. Hyde, Melissa Krueger, Robb W. Glenny, Matthew Bruce, Christoph P. Hofstetter
Traumatic spinal cord injury (tSCI) causes an immediate loss of neurological function, and the prediction of recovery is difficult in the acute phase. In this study, we used contrast-enhanced ultrasound imaging to quantify intraspinal vascular disruption acutely after tSCI. In a rodent thoracic tSCI model, contrast-enhanced ultrasound revealed a perfusion area deficit that was positively correlated with injury severity and negatively correlated with hindlimb locomotor function at 8 weeks after injury. The spinal perfusion index was calculated by normalizing the contrast inflow at the injury center to the contrast inflow in the injury periphery. The spinal perfusion index decreased with increasing injury severity and positively correlated with hindlimb locomotor function at 8 weeks after injury. The feasibility of intraoperative contrast-enhanced ultrasound imaging was further tested in a cohort of 27 patients with acute tSCI of varying severity and including both motor-complete and motor-incomplete tSCIs. Both the perfusion area deficit and spinal perfusion index were different between motor-complete and motor-incomplete patients. Moreover, the perfusion area deficit and spinal perfusion index correlated with the injury severity at intake and exhibited a correlation with extent of functional recovery at 6 months. Our data suggest that intraoperative contrast-enhanced, ultrasound-derived metrics are correlated with injury severity and chronic functional outcome after tSCI. Larger clinical studies are required to better assess the reliability of the proposed contrast-enhanced ultrasound biomarkers and their prognostic capacity.
创伤性脊髓损伤(tSCI)会导致神经功能立即丧失,而在急性期很难预测恢复情况。在这项研究中,我们使用对比增强超声成像技术来量化创伤性脊髓损伤后急性期的椎管内血管破坏情况。在啮齿类动物胸椎创伤后脊髓损伤模型中,对比增强超声显示了灌注区的缺损,该缺损与损伤严重程度呈正相关,与损伤后 8 周的后肢运动功能呈负相关。脊髓灌注指数的计算方法是将损伤中心的造影剂流入量与损伤周边的造影剂流入量归一化。脊髓灌注指数随着损伤严重程度的增加而降低,并与损伤后8周的后肢运动功能呈正相关。术中对比增强超声成像的可行性在一组 27 例不同严重程度的急性 tSCI 患者(包括运动完全性和运动不完全性 tSCI)中进行了进一步测试。运动完全性和运动不完全性患者的灌注面积缺失和脊髓灌注指数均不同。此外,灌注面积缺损和脊髓灌注指数与入院时的损伤严重程度相关,并与6个月时的功能恢复程度相关。我们的数据表明,术中对比增强超声衍生指标与创伤后脊髓损伤的严重程度和慢性功能预后相关。需要进行更大规模的临床研究,以更好地评估所提出的对比增强超声生物标志物的可靠性及其预后能力。
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引用次数: 0
ARGX-119 is an agonist antibody for human MuSK that reverses disease relapse in a mouse model of congenital myasthenic syndrome ARGX-119 是一种人 MuSK 激动剂抗体,可逆转先天性肌无力综合征小鼠模型的疾病复发
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-18 DOI: 10.1126/scitranslmed.ado7189
Roeland Vanhauwaert, Julien Oury, Bernhardt Vankerckhoven, Christophe Steyaert, Stine Marie Jensen, Dana L. E. Vergoossen, Christa Kneip, Leah Santana, Jamie L. Lim, Jaap J. Plomp, Roy Augustinus, Shohei Koide, Christophe Blanchetot, Peter Ulrichts, Maartje G. Huijbers, Karen Silence, Steven J. Burden
Muscle-specific kinase (MuSK) is essential for the formation, function, and preservation of neuromuscular synapses. Activation of MuSK by a MuSK agonist antibody may stabilize or improve the function of the neuromuscular junction (NMJ) in patients with disorders of the NMJ, such as congenital myasthenia (CM). Here, we generated and characterized ARGX-119, a first-in-class humanized agonist monoclonal antibody specific for MuSK, that is being developed for treatment of patients with neuromuscular diseases. We performed in vitro ligand-binding assays to show that ARGX-119 binds with high affinity to the Frizzled-like domain of human, nonhuman primate, rat, and mouse MuSK, without off-target binding, making it suitable for clinical development. Within the Fc region, ARGX-119 harbors L234A and L235A mutations to diminish potential immune-activating effector functions. Its mode of action is to activate MuSK, without interfering with its natural ligand neural Agrin, and cluster acetylcholine receptors in a dose-dependent manner, thereby stabilizing neuromuscular function. In a mouse model of DOK7 CM, ARGX-119 prevented early postnatal lethality and reversed disease relapse in adult Dok7 CM mice by restoring neuromuscular function and reducing muscle weakness and fatigability in a dose-dependent manner. Pharmacokinetic studies in nonhuman primates, rats, and mice revealed a nonlinear PK behavior of ARGX-119, indicative of target-mediated drug disposition and in vivo target engagement. On the basis of this proof-of-concept study, ARGX-119 has the potential to alleviate neuromuscular diseases hallmarked by impaired neuromuscular synaptic function, warranting further clinical development.
肌肉特异性激酶(MuSK)对神经肌肉突触的形成、功能和保存至关重要。通过 MuSK 激动剂抗体激活 MuSK 可稳定或改善神经肌肉接头(NMJ)功能,如先天性肌萎缩症(CM)患者。在这里,我们生成并鉴定了 ARGX-119,这是一种特异于 MuSK 的首类人源化激动剂单克隆抗体,目前正在开发用于治疗神经肌肉疾病患者。我们进行了体外配体结合试验,结果表明 ARGX-119 能与人类、非人灵长类动物、大鼠和小鼠 MuSK 的 Frizzled 样结构域高亲和力结合,且无脱靶结合,因此适合临床开发。在 Fc 区域,ARGX-119 存在 L234A 和 L235A 突变,从而削弱了潜在的免疫激活效应功能。它的作用模式是激活 MuSK,而不干扰其天然配体 Agrin,并以剂量依赖性方式集聚乙酰胆碱受体,从而稳定神经肌肉功能。在一种 DOK7 CM 小鼠模型中,ARGX-119 通过恢复神经肌肉功能并以剂量依赖性方式减少肌无力和疲劳,防止了出生后早期致死,并逆转了成年 Dok7 CM 小鼠的疾病复发。在非人灵长类动物、大鼠和小鼠体内进行的药代动力学研究显示,ARGX-119 的 PK 行为是非线性的,表明了靶点介导的药物处置和体内靶点参与。在这项概念验证研究的基础上,ARGX-119 有可能缓解以神经肌肉突触功能受损为特征的神经肌肉疾病,值得进一步临床开发。
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引用次数: 0
Deep humoral profiling coupled to interpretable machine learning unveils diagnostic markers and pathophysiology of schistosomiasis 深度体液分析与可解释的机器学习相结合,揭示血吸虫病的诊断标记和病理生理学
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-18 DOI: 10.1126/scitranslmed.adk7832
Anushka Saha, Trirupa Chakraborty, Javad Rahimikollu, Hanxi Xiao, Lorena B. Pereira de Oliveira, Timothy W. Hand, Sukwan Handali, W. Evan Secor, Lucia A. O. Fraga, Jessica K. Fairley, Jishnu Das, Aniruddh Sarkar
Schistosomiasis, a highly prevalent parasitic disease, affects more than 200 million people worldwide. Current diagnostics based on parasite egg detection in stool detect infection only at a late stage, and current antibody-based tests cannot distinguish past from current infection. Here, we developed and used a multiplexed antibody profiling platform to obtain a comprehensive repertoire of antihelminth humoral profiles including isotype, subclass, Fc receptor (FcR) binding, and glycosylation profiles of antigen-specific antibodies. Using Essential Regression (ER) and SLIDE, interpretable machine learning methods, we identified latent factors (context-specific groups) that move beyond biomarkers and provide insights into the pathophysiology of different stages of schistosome infection. By comparing profiles of infected and healthy individuals, we identified modules with unique humoral signatures of active disease, including hallmark signatures of parasitic infection such as elevated immunoglobulin G4 (IgG4). However, we also captured previously uncharacterized humoral responses including elevated FcR binding and specific antibody glycoforms in patients with active infection, helping distinguish them from those without active infection but with equivalent antibody titers. This signature was validated in an independent cohort. Our approach also uncovered two distinct endotypes, nonpatent infection and prior infection, in those who were not actively infected. Higher amounts of IgG1 and FcR1/FcR3A binding were also found to be likely protective of the transition from nonpatent to active infection. Overall, we unveiled markers for antibody-based diagnostics and latent factors underlying the pathogenesis of schistosome infection. Our results suggest that selective antigen targeting could be useful in early detection, thus controlling infection severity.
血吸虫病是一种高度流行的寄生虫病,影响着全球 2 亿多人。目前基于粪便中寄生虫卵检测的诊断方法只能检测到晚期感染,而目前基于抗体的检测方法无法区分过去和现在的感染。在这里,我们开发并使用了一个多重抗体图谱平台,以获得全面的抗蠕虫体液图谱,包括抗原特异性抗体的同型、亚类、Fc受体(FcR)结合和糖基化图谱。利用基本回归(ER)和可解释的机器学习方法 SLIDE,我们确定了超越生物标志物的潜伏因素(特定环境组),并深入了解了血吸虫感染不同阶段的病理生理学。通过比较感染者和健康人的特征,我们确定了具有活动性疾病独特体液特征的模块,包括寄生虫感染的标志性特征,如免疫球蛋白 G4 (IgG4) 升高。不过,我们也捕捉到了以前未曾描述过的体液反应,包括活动性感染患者中升高的 FcR 结合力和特异性抗体糖形,这有助于将他们与没有活动性感染但抗体滴度相当的患者区分开来。这一特征在一个独立的队列中得到了验证。我们的方法还在非活动性感染者中发现了两种不同的内型,即非专利感染和既往感染。我们还发现,较高的 IgG1 和 FcR1/FcR3A 结合量可能对从非专利感染到活动性感染的转变具有保护作用。总之,我们揭示了基于抗体的诊断标记和血吸虫感染发病机制的潜在因素。我们的研究结果表明,选择性抗原靶向可用于早期检测,从而控制感染的严重程度。
{"title":"Deep humoral profiling coupled to interpretable machine learning unveils diagnostic markers and pathophysiology of schistosomiasis","authors":"Anushka Saha,&nbsp;Trirupa Chakraborty,&nbsp;Javad Rahimikollu,&nbsp;Hanxi Xiao,&nbsp;Lorena B. Pereira de Oliveira,&nbsp;Timothy W. Hand,&nbsp;Sukwan Handali,&nbsp;W. Evan Secor,&nbsp;Lucia A. O. Fraga,&nbsp;Jessica K. Fairley,&nbsp;Jishnu Das,&nbsp;Aniruddh Sarkar","doi":"10.1126/scitranslmed.adk7832","DOIUrl":"10.1126/scitranslmed.adk7832","url":null,"abstract":"<div >Schistosomiasis, a highly prevalent parasitic disease, affects more than 200 million people worldwide. Current diagnostics based on parasite egg detection in stool detect infection only at a late stage, and current antibody-based tests cannot distinguish past from current infection. Here, we developed and used a multiplexed antibody profiling platform to obtain a comprehensive repertoire of antihelminth humoral profiles including isotype, subclass, Fc receptor (FcR) binding, and glycosylation profiles of antigen-specific antibodies. Using Essential Regression (ER) and SLIDE, interpretable machine learning methods, we identified latent factors (context-specific groups) that move beyond biomarkers and provide insights into the pathophysiology of different stages of schistosome infection. By comparing profiles of infected and healthy individuals, we identified modules with unique humoral signatures of active disease, including hallmark signatures of parasitic infection such as elevated immunoglobulin G4 (IgG4). However, we also captured previously uncharacterized humoral responses including elevated FcR binding and specific antibody glycoforms in patients with active infection, helping distinguish them from those without active infection but with equivalent antibody titers. This signature was validated in an independent cohort. Our approach also uncovered two distinct endotypes, nonpatent infection and prior infection, in those who were not actively infected. Higher amounts of IgG1 and FcR1/FcR3A binding were also found to be likely protective of the transition from nonpatent to active infection. Overall, we unveiled markers for antibody-based diagnostics and latent factors underlying the pathogenesis of schistosome infection. Our results suggest that selective antigen targeting could be useful in early detection, thus controlling infection severity.</div>","PeriodicalId":21580,"journal":{"name":"Science Translational Medicine","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preschool-age children maintain a distinct memory CD4+ T cell and memory B cell response after SARS-CoV-2 infection 学龄前儿童在感染非典-CoV-2 病毒后可保持不同的记忆 CD4+ T 细胞和记忆 B 细胞反应
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-18 DOI: 10.1126/scitranslmed.adl1997
Benoît Manfroi, Bui Thi Cuc, Aurélien Sokal, Alexis Vandenberghe, Sarah Temmam, Mikaël Attia, Mohamed El Behi, Francesco Camaglia, Ngan Thu Nguyen, Jelka Pohar, Layale Salem-Wehbe, Valentine Pottez-Jouatte, Sibyline Borzakian, Narcisse Elenga, Caroline Galeotti, Guillaume Morelle, Camille de truchis de Lays, Michaela Semeraro, Anne-Sophie Romain, Mélodie Aubart, Naim Ouldali, Florence Mahuteau-Betzer, Claire Beauvineau, Elsa Amouyal, Romain Berthaud, Célia Crétolle, Marc Duval Arnould, Albert Faye, Mathie Lorrot, Grégoire Benoist, Nelly Briand, Marie Courbebaisse, Roland Martin, Peter Van Endert, Jean-Sébastien Hulot, Anne Blanchard, Eric Tartour, Maria Leite-de-Moraes, Guillaume Lezmi, Mickael Ménager, Marine Luka, Claude-Agnès Reynaud, Jean-Claude Weill, Laetitia Languille, Marc Michel, Pascal Chappert, Thierry Mora, Aleksandra M. Walczak, Marc Eloit, Petra Bacher, Alexander Scheffold, Matthieu Mahévas, Isabelle Sermet-Gaudelus, Simon Fillatreau
The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–reactive CD3+CD4+CD154+ T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4+ T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4+ T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2–reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner.
人类免疫系统的发育在出生后持续数年。这一成熟阶段对适应性免疫质量和幼年感染后获得免疫记忆的影响仍未完全明确。在这里,我们使用抗原反应性 T 细胞(ARTE)检测法和多维流式细胞术分析了感染前、感染期间和感染后 11 个月儿童和成人中循环的严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)反应性 CD3+CD4+CD154+ T 细胞,并根据疾病严重程度将儿童和成人分为不同的年龄组。在感染 SARS-CoV-2 期间,5 岁以下儿童的 CD4+ T 细胞抗病毒反应较低,而 5 岁以上儿童和轻症成人的 CD4+ T 细胞对病毒的反应在数量和表型上具有可比性。重症成人的反应特点是病毒反应性促炎性和细胞毒性 T 细胞的频率较高。学龄前儿童在感染 SARS-CoV-2 后,不仅能维持与成人相当的中和 SARS-CoV-2 反应抗体,而且记忆 T 细胞的表型也与成人不同,具有高度炎症特征和向炎症部位迁移的特性。此外,与其他组群相比,学龄前儿童的循环病毒反应记忆 B 细胞明显较少。总之,我们的研究结果揭示了人类幼年抗病毒免疫的独特面貌,并表明针对 SARS-CoV-2 的适应性反应的成熟是以渐进的方式进行的,其特征与成人相似。
{"title":"Preschool-age children maintain a distinct memory CD4+ T cell and memory B cell response after SARS-CoV-2 infection","authors":"Benoît Manfroi,&nbsp;Bui Thi Cuc,&nbsp;Aurélien Sokal,&nbsp;Alexis Vandenberghe,&nbsp;Sarah Temmam,&nbsp;Mikaël Attia,&nbsp;Mohamed El Behi,&nbsp;Francesco Camaglia,&nbsp;Ngan Thu Nguyen,&nbsp;Jelka Pohar,&nbsp;Layale Salem-Wehbe,&nbsp;Valentine Pottez-Jouatte,&nbsp;Sibyline Borzakian,&nbsp;Narcisse Elenga,&nbsp;Caroline Galeotti,&nbsp;Guillaume Morelle,&nbsp;Camille de truchis de Lays,&nbsp;Michaela Semeraro,&nbsp;Anne-Sophie Romain,&nbsp;Mélodie Aubart,&nbsp;Naim Ouldali,&nbsp;Florence Mahuteau-Betzer,&nbsp;Claire Beauvineau,&nbsp;Elsa Amouyal,&nbsp;Romain Berthaud,&nbsp;Célia Crétolle,&nbsp;Marc Duval Arnould,&nbsp;Albert Faye,&nbsp;Mathie Lorrot,&nbsp;Grégoire Benoist,&nbsp;Nelly Briand,&nbsp;Marie Courbebaisse,&nbsp;Roland Martin,&nbsp;Peter Van Endert,&nbsp;Jean-Sébastien Hulot,&nbsp;Anne Blanchard,&nbsp;Eric Tartour,&nbsp;Maria Leite-de-Moraes,&nbsp;Guillaume Lezmi,&nbsp;Mickael Ménager,&nbsp;Marine Luka,&nbsp;Claude-Agnès Reynaud,&nbsp;Jean-Claude Weill,&nbsp;Laetitia Languille,&nbsp;Marc Michel,&nbsp;Pascal Chappert,&nbsp;Thierry Mora,&nbsp;Aleksandra M. Walczak,&nbsp;Marc Eloit,&nbsp;Petra Bacher,&nbsp;Alexander Scheffold,&nbsp;Matthieu Mahévas,&nbsp;Isabelle Sermet-Gaudelus,&nbsp;Simon Fillatreau","doi":"10.1126/scitranslmed.adl1997","DOIUrl":"10.1126/scitranslmed.adl1997","url":null,"abstract":"<div >The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–reactive CD3<sup>+</sup>CD4<sup>+</sup>CD154<sup>+</sup> T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4<sup>+</sup> T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4<sup>+</sup> T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2–reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner.</div>","PeriodicalId":21580,"journal":{"name":"Science Translational Medicine","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeted partial reprogramming of age-associated cell states improves markers of health in mouse models of aging 对衰老相关细胞状态进行有针对性的部分重编程可改善衰老小鼠模型的健康指标
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1126/scitranslmed.adg1777
Sanjeeb Kumar Sahu, Pradeep Reddy, Jinlong Lu, Yanjiao Shao, Chao Wang, Mako Tsuji, Estrella Nuñez Delicado, Concepcion Rodriguez Esteban, Juan Carlos Izpisua Belmonte
Aging is a complex multifactorial process associated with epigenome dysregulation, increased cellular senescence, and decreased rejuvenation capacity. Short-term cyclic expression of octamer-binding transcription factor 4 (Oct4), sex-determining region Y-box 2 (Sox2), Kruppel-like factor 4 (Klf4), and cellular myelocytomatosis oncogene (cMyc) (OSKM) in wild-type mice improves health but fails to distinguish cell states, posing risks to healthy cells. Here, we delivered a single dose of adeno-associated viruses (AAVs) harboring OSK under the control of the cyclin-dependent kinase inhibitor 2a (Cdkn2a) promoter to specifically partially reprogram aged and stressed cells in a mouse model of Hutchinson-Gilford progeria syndrome (HGPS). Mice showed reduced expression of proinflammatory cytokines and extended life spans upon aged cell–specific OSK expression. The bone marrow and spleen, in particular, showed pronounced gene expression changes, and partial reprogramming in aged HGPS mice led to a shift in the cellular composition of the hematopoietic stem cell compartment toward that of young mice. Administration of AAVs carrying Cdkn2a-OSK to naturally aged wild-type mice also delayed aging phenotypes and extended life spans without altering the incidence of tumor development. Furthermore, intradermal injection of AAVs carrying Cdkn2a-OSK led to improved wound healing in aged wild-type mice. Expression of CDKN2A-OSK in aging or stressed human primary fibroblasts led to reduced expression of inflammation-related genes but did not alter the expression of cell cycle–related genes. This targeted partial reprogramming approach may therefore facilitate the development of strategies to improve health and life span and enhance resilience in the elderly.
衰老是一个复杂的多因素过程,与表观基因组失调、细胞衰老加剧和再生能力下降有关。在野生型小鼠体内短期循环表达八聚体结合转录因子 4(Oct4)、性别决定区 Y-box 2(Sox2)、Kruppel 样因子 4(Klf4)和细胞髓母细胞瘤病癌基因(cMyc)(OSKM)可改善健康状况,但无法区分细胞状态,从而给健康细胞带来风险。在这里,我们在细胞周期蛋白依赖性激酶抑制剂2a(Cdkn2a)启动子的控制下释放了单剂量的携带OSK的腺相关病毒(AAV),在哈钦森-吉尔福特早衰综合征(HGPS)小鼠模型中特异性地对衰老和受压细胞进行了部分重编程。老化细胞特异性 OSK 表达后,小鼠促炎细胞因子的表达减少,寿命延长。尤其是骨髓和脾脏的基因表达发生了明显变化,老年 HGPS 小鼠的部分重编程导致造血干细胞区的细胞组成向年轻小鼠的细胞组成转变。给自然衰老的野生型小鼠注射携带Cdkn2a-OSK的AAV也能延缓衰老表型,延长寿命,但不会改变肿瘤的发病率。此外,皮内注射携带 Cdkn2a-OSK 的 AAV 还能改善老年野生型小鼠的伤口愈合。在衰老或受压的人类原代成纤维细胞中表达 CDKN2A-OSK 可减少炎症相关基因的表达,但不会改变细胞周期相关基因的表达。因此,这种有针对性的部分重编程方法可能有助于开发改善老年人健康和寿命以及增强复原力的策略。
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引用次数: 0
Erratum for the Research Article “Adjuvantation of a SARS-CoV-2 mRNA vaccine with controlled tissue-specific expression of an mRNA encoding IL-12p70” by B. Brook et al. 对 B. Brook 等人的研究文章 "用编码 IL-12p70 的 mRNA 的可控组织特异性表达对 SARS-CoV-2 mRNA 疫苗进行佐剂处理 "的勘误。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1126/scitranslmed.ads5741
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引用次数: 0
Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models 罗库溴铵特异性抗体会导致围手术期过敏性休克,但在临床前模型中也能起到逆转作用
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1126/scitranslmed.ado4463
Alice Dejoux, Qianqian Zhu, Christelle Ganneau, Odile Richard-Le Goff, Ophélie Godon, Julien Lemaitre, Francis Relouzat, François Huetz, Aurélien Sokal, Alexis Vandenberghe, Cyprien Pecalvel, Lise Hunault, Thomas Derenne, Caitlin M. Gillis, Bruno Iannascoli, Yidan Wang, Thierry Rose, Christel Mertens, Pascale Nicaise-Roland, NASA Study Group, Patrick England, Matthieu Mahévas, Luc de Chaisemartin, Roger Le Grand, Hélène Letscher, Frederick Saul, Cédric Pissis, Ahmed Haouz, Laurent L. Reber, Pascal Chappert, Friederike Jönsson, Didier G. Ebo, Gaël A. Millot, Sylvie Bay, Sylvie Chollet-Martin, Aurélie Gouel-Chéron, Pierre Bruhns
Neuromuscular blocking agents (NMBAs) relax skeletal muscles to facilitate surgeries and ease intubation but can lead to adverse reactions, including complications because of postoperative residual neuromuscular blockade (rNMB) and, in rare cases, anaphylaxis. Both adverse reactions vary between types of NMBAs, with rocuronium, a widely used nondepolarizing NMBA, inducing one of the longest rNMB durations and highest anaphylaxis incidences. rNMB induced by rocuronium can be reversed by the synthetic γ-cyclodextrin sugammadex. However, in rare cases, sugammadex can provoke anaphylaxis. Thus, additional therapeutic options are needed. Rocuronium-induced anaphylaxis is proposed to rely on preexisting rocuronium-binding antibodies. To understand the pathogenesis of rocuronium-induced anaphylaxis and to identify potential therapeutics, we investigated the memory B cell antibody repertoire of patients with suspected hypersensitivity to rocuronium. We identified polyclonal antibody repertoires with a high diversity among V(D)J genes without evidence of clonal groups. When recombinantly expressed, these antibodies demonstrated specificity and low affinity for rocuronium without cross-reactivity for other NMBAs. Moreover, when these antibodies were expressed as human immunoglobulin E (IgE), they triggered human mast cell activation and passive systemic anaphylaxis in transgenic mice, although their affinities were insufficient to serve as reversal agents. Rocuronium-specific, high-affinity antibodies were thus isolated from rocuronium-immunized mice. The highest-affinity antibody was able to reverse rocuronium-induced neuromuscular blockade in nonhuman primates with kinetics comparable to that of sugammadex. Together, these data support the hypothesis that antibodies cause anaphylactic reactions to rocuronium and pave the way for improved diagnostics and neuromuscular blockade reversal agents.
神经肌肉阻滞剂(NMBA)可放松骨骼肌,从而促进手术并方便插管,但也可能导致不良反应,包括术后残留神经肌肉阻滞(rNMB)引起的并发症,以及极少数情况下的过敏性休克。这两种不良反应因 NMBA 类型而异,其中罗库溴铵是一种广泛使用的非去极化 NMBA,可诱导最长的 rNMB 持续时间和最高的过敏性休克发生率。然而,在极少数情况下,苏甘麦得可引发过敏性休克。因此,还需要其他治疗方案。有人提出,罗库溴铵诱发的过敏性休克依赖于预先存在的罗库溴铵结合抗体。为了了解罗库溴铵诱发过敏性休克的发病机理并确定潜在的治疗方法,我们研究了疑似对罗库溴铵过敏的患者的记忆 B 细胞抗体复合物。我们发现多克隆抗体复合物在 V(D)J 基因间具有高度多样性,但没有克隆群的迹象。重组表达时,这些抗体对罗库溴铵表现出特异性和低亲和性,而对其他 NMBAs 没有交叉反应。此外,当这些抗体表达为人类免疫球蛋白 E(IgE)时,它们会引发转基因小鼠的人类肥大细胞活化和被动性全身过敏性休克,尽管它们的亲和力不足以作为逆转剂。因此,我们从免疫了罗库溴铵的小鼠体内分离出了罗库溴铵特异性高亲和力抗体。亲和力最高的抗体能够逆转非人灵长类动物中洛库铵诱导的神经肌肉阻滞,其动力学与苏加麦克斯相当。这些数据共同支持了抗体导致罗库溴铵过敏反应的假设,并为改进诊断和神经肌肉阻滞逆转剂铺平了道路。
{"title":"Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models","authors":"Alice Dejoux,&nbsp;Qianqian Zhu,&nbsp;Christelle Ganneau,&nbsp;Odile Richard-Le Goff,&nbsp;Ophélie Godon,&nbsp;Julien Lemaitre,&nbsp;Francis Relouzat,&nbsp;François Huetz,&nbsp;Aurélien Sokal,&nbsp;Alexis Vandenberghe,&nbsp;Cyprien Pecalvel,&nbsp;Lise Hunault,&nbsp;Thomas Derenne,&nbsp;Caitlin M. Gillis,&nbsp;Bruno Iannascoli,&nbsp;Yidan Wang,&nbsp;Thierry Rose,&nbsp;Christel Mertens,&nbsp;Pascale Nicaise-Roland,&nbsp;NASA Study Group,&nbsp;Patrick England,&nbsp;Matthieu Mahévas,&nbsp;Luc de Chaisemartin,&nbsp;Roger Le Grand,&nbsp;Hélène Letscher,&nbsp;Frederick Saul,&nbsp;Cédric Pissis,&nbsp;Ahmed Haouz,&nbsp;Laurent L. Reber,&nbsp;Pascal Chappert,&nbsp;Friederike Jönsson,&nbsp;Didier G. Ebo,&nbsp;Gaël A. Millot,&nbsp;Sylvie Bay,&nbsp;Sylvie Chollet-Martin,&nbsp;Aurélie Gouel-Chéron,&nbsp;Pierre Bruhns","doi":"10.1126/scitranslmed.ado4463","DOIUrl":"10.1126/scitranslmed.ado4463","url":null,"abstract":"<div >Neuromuscular blocking agents (NMBAs) relax skeletal muscles to facilitate surgeries and ease intubation but can lead to adverse reactions, including complications because of postoperative residual neuromuscular blockade (rNMB) and, in rare cases, anaphylaxis. Both adverse reactions vary between types of NMBAs, with rocuronium, a widely used nondepolarizing NMBA, inducing one of the longest rNMB durations and highest anaphylaxis incidences. rNMB induced by rocuronium can be reversed by the synthetic γ-cyclodextrin sugammadex. However, in rare cases, sugammadex can provoke anaphylaxis. Thus, additional therapeutic options are needed. Rocuronium-induced anaphylaxis is proposed to rely on preexisting rocuronium-binding antibodies. To understand the pathogenesis of rocuronium-induced anaphylaxis and to identify potential therapeutics, we investigated the memory B cell antibody repertoire of patients with suspected hypersensitivity to rocuronium. We identified polyclonal antibody repertoires with a high diversity among V(D)J genes without evidence of clonal groups. When recombinantly expressed, these antibodies demonstrated specificity and low affinity for rocuronium without cross-reactivity for other NMBAs. Moreover, when these antibodies were expressed as human immunoglobulin E (IgE), they triggered human mast cell activation and passive systemic anaphylaxis in transgenic mice, although their affinities were insufficient to serve as reversal agents. Rocuronium-specific, high-affinity antibodies were thus isolated from rocuronium-immunized mice. The highest-affinity antibody was able to reverse rocuronium-induced neuromuscular blockade in nonhuman primates with kinetics comparable to that of sugammadex. Together, these data support the hypothesis that antibodies cause anaphylactic reactions to rocuronium and pave the way for improved diagnostics and neuromuscular blockade reversal agents.</div>","PeriodicalId":21580,"journal":{"name":"Science Translational Medicine","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
BATF is a major driver of NK cell epigenetic reprogramming and dysfunction in AML BATF 是急性髓细胞性白血病中 NK 细胞表观遗传重编程和功能障碍的主要驱动因素
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1126/scitranslmed.adp0004
Bijender Kumar, Anand Singh, Rafet Basar, Nadima Uprety, Ye Li, Huihui Fan, Ana Karen Nunez Cortes, Mecit Kaplan, Sunil Acharya, Hila Shaim, Anna C Xu, Manrong Wu, Emily Ensley, Dexing Fang, Pinaki P. Banerjee, Luciana Melo Garcia, Silvia Tiberti, Paul Lin, Hind Rafei, Maliha Nuzhat Munir, Madison Moore, Mayra Shanley, Mayela Mendt, Lucila N. Kerbauy, Bin Liu, Alexander Biederstädt, Elif Gokdemir, Susmita Ghosh, Kiran Kundu, Francia Reyes-Silva, Xin Ru Jiang, Xinhai Wan, April L. Gilbert, Merve Dede, Vakul Mohanty, Jinzhuang Dou, Patrick Zhang, Enli Liu, Luis Muniz-Feliciano, Gary M. Deyter, Abhinav K. Jain, Juan Jose Rodriguez-Sevilla, Simona Colla, Guillermo Garcia-Manero, Elizabeth J. Shpall, Ken Chen, Hussein A. Abbas, Kunal Rai, Katayoun Rezvani, May Daher
Myelodysplastic syndrome and acute myeloid leukemia (AML) belong to a continuous disease spectrum of myeloid malignancies with poor prognosis in the relapsed/refractory setting necessitating novel therapies. Natural killer (NK) cells from patients with myeloid malignancies display global dysfunction with impaired killing capacity, altered metabolism, and an exhausted phenotype at the single-cell transcriptomic and proteomic levels. In this study, we identified that this dysfunction was mediated through a cross-talk between NK cells and myeloid blasts necessitating cell-cell contact. NK cell dysfunction could be prevented by targeting the αvβ-integrin/TGF-β/SMAD pathway but, once established, was persistent because of profound epigenetic reprogramming. We identified BATF as a core transcription factor and the main mediator of this NK cell dysfunction in AML. Mechanistically, we found that BATF was directly regulated and induced by SMAD2/3 and, in turn, bound to key genes related to NK cell exhaustion, such as HAVCR2, LAG3, TIGIT, and CTLA4. BATF deletion enhanced NK cell function against AML in vitro and in vivo. Collectively, our findings reveal a previously unidentified mechanism of NK immune evasion in AML manifested by epigenetic rewiring and inactivation of NK cells by myeloid blasts. This work highlights the importance of using healthy allogeneic NK cells as an adoptive cell therapy to treat patients with myeloid malignancies combined with strategies aimed at preventing the dysfunction by targeting the TGF-β pathway or BATF.
骨髓增生异常综合征和急性髓性白血病(AML)属于髓系恶性肿瘤的一种连续性疾病谱,在复发/难治的情况下预后较差,需要采用新型疗法。髓系恶性肿瘤患者的自然杀伤(NK)细胞表现出整体功能障碍,杀伤能力受损,新陈代谢改变,在单细胞转录组和蛋白质组水平上表现出衰竭表型。在这项研究中,我们发现这种功能障碍是通过 NK 细胞和髓样细胞之间的交叉对话介导的,而这种交叉对话需要细胞与细胞之间的接触。NK细胞功能障碍可通过靶向αvβ-整合素/TGF-β/SMAD通路来预防,但一旦形成,就会因深刻的表观遗传重编程而持续存在。我们发现 BATF 是一种核心转录因子,也是导致 AML 中 NK 细胞功能障碍的主要介质。从机理上讲,我们发现BATF直接受SMAD2/3调控和诱导,进而与NK细胞衰竭相关的关键基因结合,如HAVCR2、LAG3、TIGIT和CTLA4。删除 BATF 可增强 NK 细胞在体外和体内抗 AML 的功能。总之,我们的研究结果揭示了一种之前未被发现的NK免疫在AML中的逃避机制,这种机制表现为NK细胞的表观遗传重排和髓细胞瘤的失活。这项工作强调了使用健康的异体NK细胞作为治疗髓系恶性肿瘤患者的采纳性细胞疗法的重要性,同时还强调了通过靶向TGF-β途径或BATF来预防功能障碍的策略的重要性。
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引用次数: 0
A spatially resolved single-cell lung atlas integrated with clinical and blood signatures distinguishes COVID-19 disease trajectories 结合临床和血液特征的空间分辨单细胞肺图谱可区分 COVID-19 疾病轨迹
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1126/scitranslmed.adk9149
João Da Silva Filho, Vanessa Herder, Matthew P. Gibbins, Monique Freire dos Reis, Gisely Cardoso Melo, Michael J. Haley, Carla Cristina Judice, Fernando Fonseca Almeida Val, Mayla Borba, Tatyana Almeida Tavella, Vanderson de Sousa Sampaio, Charalampos Attipa, Fiona McMonagle, Derek Wright, Marcus Vinicius Guimaraes de Lacerda, Fabio Trindade Maranhão Costa, Kevin N. Couper, Wuelton Marcelo Monteiro, Luiz Carlos de Lima Ferreira, Christopher Alan Moxon, Massimo Palmarini, Matthias Marti
COVID-19 is characterized by a broad range of symptoms and disease trajectories. Understanding the correlation between clinical biomarkers and lung pathology during acute COVID-19 is necessary to understand its diverse pathogenesis and inform more effective treatments. Here, we present an integrated analysis of longitudinal clinical parameters, peripheral blood markers, and lung pathology in 142 Brazilian patients hospitalized with COVID-19. We identified core clinical and peripheral blood signatures differentiating disease progression between patients who recovered from severe disease compared with those who succumbed to the disease. Signatures were heterogeneous among fatal cases yet clustered into two patient groups: “early death” (<15 days until death) and “late death” (>15 days). Progression to early death was characterized systemically and in lung histopathological samples by rapid endothelial and myeloid activation and the presence of thrombi associated with SARS-CoV-2+ macrophages. In contrast, progression to late death was associated with fibrosis, apoptosis, and SARS-CoV-2+ epithelial cells in postmortem lung tissue. In late death cases, cytotoxicity, interferon, and T helper 17 (TH17) signatures were only detectable in the peripheral blood after 2 weeks of hospitalization. Progression to recovery was associated with higher lymphocyte counts, TH2 responses, and anti-inflammatory–mediated responses. By integrating antemortem longitudinal blood signatures and spatial single-cell lung signatures from postmortem lung samples, we defined clinical parameters that could be used to help predict COVID-19 outcomes.
COVID-19 的特点是症状和疾病轨迹多种多样。了解急性 COVID-19 期间临床生物标志物与肺部病理之间的相关性对于了解其多样化的发病机制和提供更有效的治疗方法非常必要。在此,我们对 142 名巴西 COVID-19 住院患者的纵向临床参数、外周血标志物和肺部病理进行了综合分析。我们发现了核心临床和外周血特征,这些特征区分了重症康复患者和死亡患者的疾病进展。死亡病例的特征不尽相同,但可分为两组:"早期死亡"(15 天内死亡)和 "晚期死亡"(15 天内死亡)。在全身和肺组织病理学样本中,早期死亡的特征是内皮细胞和髓细胞迅速活化,并出现与 SARS-CoV-2+ 巨噬细胞相关的血栓。与此相反,晚期死亡的进展与纤维化、凋亡和死后肺组织中的 SARS-CoV-2+ 上皮细胞有关。在晚期死亡病例中,只有在住院两周后才能在外周血中检测到细胞毒性、干扰素和T辅助细胞17(TH17)特征。恢复期的进展与较高的淋巴细胞计数、TH2 反应和抗炎介导的反应有关。通过整合死前纵向血液特征和死后肺样本的空间单细胞肺特征,我们定义了可用于帮助预测 COVID-19 结果的临床参数。
{"title":"A spatially resolved single-cell lung atlas integrated with clinical and blood signatures distinguishes COVID-19 disease trajectories","authors":"João Da Silva Filho,&nbsp;Vanessa Herder,&nbsp;Matthew P. Gibbins,&nbsp;Monique Freire dos Reis,&nbsp;Gisely Cardoso Melo,&nbsp;Michael J. Haley,&nbsp;Carla Cristina Judice,&nbsp;Fernando Fonseca Almeida Val,&nbsp;Mayla Borba,&nbsp;Tatyana Almeida Tavella,&nbsp;Vanderson de Sousa Sampaio,&nbsp;Charalampos Attipa,&nbsp;Fiona McMonagle,&nbsp;Derek Wright,&nbsp;Marcus Vinicius Guimaraes de Lacerda,&nbsp;Fabio Trindade Maranhão Costa,&nbsp;Kevin N. Couper,&nbsp;Wuelton Marcelo Monteiro,&nbsp;Luiz Carlos de Lima Ferreira,&nbsp;Christopher Alan Moxon,&nbsp;Massimo Palmarini,&nbsp;Matthias Marti","doi":"10.1126/scitranslmed.adk9149","DOIUrl":"10.1126/scitranslmed.adk9149","url":null,"abstract":"<div >COVID-19 is characterized by a broad range of symptoms and disease trajectories. Understanding the correlation between clinical biomarkers and lung pathology during acute COVID-19 is necessary to understand its diverse pathogenesis and inform more effective treatments. Here, we present an integrated analysis of longitudinal clinical parameters, peripheral blood markers, and lung pathology in 142 Brazilian patients hospitalized with COVID-19. We identified core clinical and peripheral blood signatures differentiating disease progression between patients who recovered from severe disease compared with those who succumbed to the disease. Signatures were heterogeneous among fatal cases yet clustered into two patient groups: “early death” (&lt;15 days until death) and “late death” (&gt;15 days). Progression to early death was characterized systemically and in lung histopathological samples by rapid endothelial and myeloid activation and the presence of thrombi associated with SARS-CoV-2<sup>+</sup> macrophages. In contrast, progression to late death was associated with fibrosis, apoptosis, and SARS-CoV-2<sup>+</sup> epithelial cells in postmortem lung tissue. In late death cases, cytotoxicity, interferon, and T helper 17 (T<sub>H</sub>17) signatures were only detectable in the peripheral blood after 2 weeks of hospitalization. Progression to recovery was associated with higher lymphocyte counts, T<sub>H</sub>2 responses, and anti-inflammatory–mediated responses. By integrating antemortem longitudinal blood signatures and spatial single-cell lung signatures from postmortem lung samples, we defined clinical parameters that could be used to help predict COVID-19 outcomes.</div>","PeriodicalId":21580,"journal":{"name":"Science Translational Medicine","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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