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ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES 姜黄和圆柏乙醇提取物对MCF-7癌细胞的抗氧化和细胞毒性作用
Pub Date : 2019-10-10 DOI: 10.26480/gws.02.2019.10.14
T. Sundram, M. Zakaria, Mohd Hamzah Mohd Nasir
Curcuma mangga and Bosenbergia rotunda which belongs to Zingiberaceae family are largely distributed in South East Asian countries including Malaysia. These plants have been traditionally used for treatment of various diseases. In this research we focused on determining the antioxidant and cytotoxic activity of C. mangga and B. rotunda ethanolic extracts. Initially, in this study, ethanolic extracts from rhizomes of C. mangga and B. rotunda were obtained through Soxhlet extraction method. 12.6% and 3.5% of crude extract were obtained from C. mangga and B. rotunda respectively. For MTT assay, the IC50 values are 207µg/ml and 31.96 µg/ml for C. mangga and B. rotunda respectively. Meanwhile for the antioxidant activity using DPPH assay, C. mangga showed higher antioxidant activity with IC50 values of 1073µg/ml compared to B. rotunda with IC50 values of 578µg/ml. It can be concluded both extracts showed low inhibitory effect on MCF-7 cancer cells growth and DPPH free radical activity.
姜科姜黄(Curcuma mangga)和姜黄(Bosenbergia rotunda)主要分布在包括马来西亚在内的东南亚国家。传统上,这些植物被用来治疗各种疾病。本文主要研究了芒草和圆藤乙醇提取物的抗氧化活性和细胞毒活性。本研究首先采用索氏提取法提取芒草根状茎和圆叶根状茎的乙醇提取物。芒草粗提物的总提取率为12.6%,圆形草粗提物的总提取率为3.5%。MTT法的IC50值分别为207µg/ml和31.96µg/ml。DPPH法测定芒草的抗氧化活性,其IC50值为1073µg/ml,高于圆形草(578µg/ml)。结果表明,两种提取物对MCF-7癌细胞生长和DPPH自由基活性均有较低的抑制作用。
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引用次数: 6
DIAGNOSTIC AND CHARACTERIZATION OF MATERIAL STATE FROM THE FIRST JEWS` ADOBE RANCH IN ALGARROBOS, CARLOS CASARES, BUENOS AIRES, ARGENTINA 阿根廷布宜诺斯艾利斯,carlos casares, algarrobos,第一个犹太人土坯牧场的物质状态诊断与表征
Pub Date : 2019-09-17 DOI: 10.26480/gws.02.2019.06.09
P. V. Alfieri, S. Zicarelli, G. Molinari, F. Iloro, Luis Pascual Traversa
In Buenos Aires, Argentina there are many architectonic heritage objects that represent the history of the population of different places with different degree of deterioration and conservation, so their study and survey were necessary to avoid the complete loss of it. During the study of heritage assets in towns, villages, and cities of Buenos Aires that the LEMIT makes more than 20 years ago, numerous architectonic heritage assets were found with different degree of deterioration and conservation. As result of some surveys, important restoration and conservation interventions were done preserving province heritage. Thus, the aim of this paper was the study of the materials deterioration state from the first ranch built by the settlement of the Jewish settlers in 1891, in Algarrobos city, Carlos Casares, Buenos Aires. This ranch was the first houses of Jews when they arrived from Russia. The material analysis here presented allowed to recognize its state of deterioration and conservation through discerning if these materials were from the mentioned age and consequently, providing fundamental information to know part of settlement history formation. On the other hand, its study allowed generating a plan of interventions and conservation in short - and medium-term.
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引用次数: 0
MAPPING MANGROVE DEGRADATION IN PAHANG RIVER ESTUARY, PEKAN PAHANG BY USING REMOTE SENSING 应用遥感绘制巴杭河口红树林退化图
Pub Date : 2019-03-28 DOI: 10.26480/GWS.02.2019.01.05
A. Zuhairi, K Zaleha, Nur Suhaila, S. Shaheed
This study examined the mangrove degradation in Pahang River Estuary, Pekan sub-district, Pahang between 1990 and 2017 using supervised classification. Landsat 8 OLI and Landsat 5 TM images of Pahang River Estuary have been analyzed using supervised classification and field survey. Based on the accuracy assessment, the confusion matrix for supervised classifications ranged from 77% to 81%. Kappa coefficient (K) for supervised classification ranged between the value of 0.67 and 0.72. The total mangrove covers in 2017 was 1,535.40 ha, decreased by 43.7% or 670.80 ha from its total area of 2,206.20 ha in 1990. The expansion of development area was observed in the past 27 years. Several other causes of mangrove degradation were identified during field survey, including sand mining activities along riverbank, mangrove clearing for aquaculture and agriculture. The rapid development within the coastal area and estuary had suppressed the mangroves propagation over the years. Long term monitoring of mangrove distribution is crucial to ensure the sustainability of the mangrove ecosystem.
本研究采用监督分类法对彭亨Pekan街道彭亨河口1990年至2017年间的红树林退化情况进行了调查。采用监督分类和实地调查相结合的方法,对彭河河口8号陆地卫星OLI和5号陆地卫星TM影像进行了分析。根据准确度评估,监督分类的混淆矩阵在77%到81%之间。监督分类的Kappa系数(K)介于0.67和0.72之间。2017年红树林总面积为1535.40公顷,比1990年的2206.20公顷减少了43.7%,即670.80公顷。在过去的27年里,人们观察到了开发面积的扩大。在实地调查中,还发现了红树林退化的其他几个原因,包括河岸边的采砂活动、为水产养殖和农业清理红树林。多年来,沿海地区和河口的快速发展抑制了红树林的繁殖。对红树林分布的长期监测对于确保红树林生态系统的可持续性至关重要。
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引用次数: 3
ANTI-DIABETIC POTENTIAL OF PEPTIDE FROM P. NIRURI REVEALS THROUGH CARBOHYDRATE HYDROLYZING ENZYME INHIBITION ASSAY 通过碳水化合物水解酶抑制实验,揭示了该菌肽的抗糖尿病潜能
Pub Date : 2019-01-07 DOI: 10.26480/gws.01.2019.17.19
Noor Hasniza Md Zin, Ainul Mardhiah Mohd Nail
Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anti-diabetic properties. In this research, protein extracts from different plant parts (leave, fruit and stem) of P. niruri were investigated for their anti-diabetic potential through α-amylase and α-glucosidase enzyme inhibition assays. For the enzyme inhibition assay, fruit was found to have the highest inhibition percentage (90.0%) against α-glucosidase followed by leave (62.6%) and stem (38.4%). The similar patterns were also recorded for the α-amylase enzyme inhibition assay, in which, fruit showed the highest inhibition percentage (64.1%) followed by leave (33.3%) and stem (18.2%). The findings of this research suggest that fruit of P. niruri is a potential plant part with regards to antidiabetic properties since it exhibits the highest inhibition activity against both α-amylase and α-glucosidase enzyme compared to leave and stem.
叶下珠(P.nirui)具有多种药理作用,包括抗糖尿病特性。在本研究中,通过α-淀粉酶和α-葡萄糖苷酶抑制试验,研究了尼鲁里不同植物部位(叶、果和茎)的蛋白质提取物抗糖尿病的潜力。在酶抑制试验中,果实对α-葡萄糖苷酶的抑制率最高(90.0%),其次是叶(62.6%)和茎(38.4%)。α-淀粉酶酶抑制试验也记录了类似的模式,其中,果实的抑制率最高(64.1%),其次是叶(33.3%)和茎(18.2%)。本研究结果表明,与叶和茎相比,尼鲁里果实对α-淀粉酶和α-葡萄糖苷酶的抑制活性最高,是一种潜在的抗糖尿病植物。
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引用次数: 1
PROTEOMICS AS TOOLS FOR BIOMARKERS DISCOVERY OF ADULTERATION IN SLAUGHTERING PROCEDURES 蛋白质组学作为生物标志物的工具屠宰过程中掺假现象的发现
Pub Date : 2019-01-07 DOI: 10.26480/GWS.01.2019.11.16
A. Amid, N. A. Samah
Slaughtering is an unavoidably sensitive issue among Muslim and Jews. This paper gives an outlook on possible detection methods in resolving the dilemma of religious slaughtering status. It will be rather easier to differentiate meat of different animal origins due to the exclusive genetic blueprint. However, in the case of adulteration in slaughtering procedure, the meat was taken from a similar source, thus, complicating the detection process. Therefore, an alternative approach employing proteomics were developed to identify protein expression patterns after external stimulation with electrical treatment. In the slaughtering process, the pain which is triggered by an external stimulus is expected to influence the protein profiles. Therefore, variations in stunning treatments which result in different patterns of protein profiles will pinpoint the specific biomarker for over stunned animals. This will inevitably help to detect adulterations in slaughtering procedure.
屠杀是穆斯林和犹太人之间不可避免的敏感问题。本文对解决宗教屠杀困境的可能检测方法进行了展望。由于独有的基因蓝图,将更容易区分不同动物来源的肉。然而,在屠宰过程中掺假的情况下,肉类来自类似的来源,因此,使检测过程复杂化。因此,开发了一种采用蛋白质组学的替代方法来鉴定外部电刺激后的蛋白质表达模式。在屠宰过程中,外界刺激引发的疼痛预计会影响蛋白质谱。因此,导致不同蛋白质谱模式的休克治疗的变化将查明过度休克动物的特定生物标志物。这将不可避免地有助于检测屠宰过程中的掺假。
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引用次数: 2
RESEARCH ON ARBITRARY VIRTUAL VIEW IMAGE SYNTHESIS METHOD OF TWO VIEWS 二视图任意虚拟视图图像合成方法的研究
Pub Date : 2019-01-07 DOI: 10.26480/GWS.01.2019.24.31
Shengyu Nan
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引用次数: 3
ANTIOXIDANT CAPACITY OF THE GREEN LEAFY VEGETABLES USING OXYGEN RADICAL ANTIOXIDANT CAPACITY (ORAC), 2,2′-AZINO-BIS (3-ETHYLBENZOTHIAZOLINE-6-SULPHONIC ACID(ABTS) AND 2,2-DIPHENYL-1-PICRYLHYDRAZYL (DPPH) ASSAYS 用氧自由基抗氧化能力(ORAC)、2,2′-叠氮基双(3-乙基苯并噻唑啉-6-磺酸)和2,2-二苯基-1-苦基肼(DPPH)测定绿叶蔬菜的抗氧化能力
Pub Date : 2019-01-07 DOI: 10.26480/GWS.01.2019.01.07
H Normah, M. J. Hanapi
Antioxidants are believed to play a very important role in the body defence system against reactive oxygen species (ROS), the harmful by-products that are generated during normal aerobic cell respiration. The objective of this study was to determine the antioxidant capacity in green leafy vegetables using ORAC, ABTS, and DPPH assays of different polyphenol fractions (free phenolic, alkaline hydrolysate, acidic hydrolysate).The antioxidant capacity of the identified free and bound phenolic acid content was measured using different assays including ORAC, ABTS, and DPPH assay (end-point assay and kinetic assay). Only hydrophilic antioxidant activities of all selected samples were examined using ORAC assay. Strong correlations were observed in acidic and alkaline hydrolysate fractions (p<0.01) as determined by ORAC and ABTS assays, respectively. In the free phenolic acid extracts, the O. basilicum (Sweet basil) ranked first, had highest antioxidant capacities of 521804±4243 µmol TE/100g DW, 329.8±0.4mg TE/g DW and 9.0±1.8 µg GAE/g DW as determined by ORAC, ABTS and DPPH, respectively. . The A. occidentale (Cashew shoot) in the alkaline hydrolysate extract showed a greatest antioxidant capacity in all three assays: 889126±7193 µmol TE/100g DW, 466.5±7.9 mg TE/g DW and 3.5±0.4 µg GAE/g DW as measured by ORAC, ABTS and DPPH, separately. While, in acidic hydrolysate, the A. occidentale (Cashew shoot) extract also dominated the antioxidant capacity with (560504±5785 µmol TE/100g DW, 387±0.7 mg TE/g DW and 5.9±0.5 µg GAE/g DW) as determined by ORAC, ABTS and DPPH assays, respectively. The acidic and basic hydrolysis yielded higher antioxidant capacities in the present study. It suggests that hydrolysis with alkaline and acidic play significant roles in liberating more phenolic acids and flavonoids and generating high antioxidant capacity in the extracts.
抗氧化剂被认为在人体防御活性氧(ROS)系统中起着非常重要的作用,活性氧是正常有氧细胞呼吸过程中产生的有害副产物。本研究的目的是利用ORAC、ABTS和DPPH测定绿叶蔬菜中不同多酚组分(游离酚、碱性水解产物和酸性水解产物)的抗氧化能力。采用ORAC、ABTS和DPPH(终点法和动力学法)等不同的测定方法测定鉴定的游离和结合酚酸含量的抗氧化能力。仅用ORAC法检测所有选定样品的亲水抗氧化活性。ORAC和ABTS检测结果显示,酸性和碱性水解产物组分之间存在显著相关性(p<0.01)。ORAC、ABTS和DPPH测定的游离酚酸提取物中,罗勒的抗氧化能力最高,分别为521804±4243µmol TE/100g DW、329.8±0.4mg TE/g DW和9.0±1.8µg GAE/g DW。在ORAC、ABTS和DPPH测试中,碱水解提取物中的a . occidentale(腰果芽)抗氧化能力最强,分别为889126±7193µmol TE/100g DW、466.5±7.9 mg TE/g DW和3.5±0.4µg GAE/g DW。而在酸性水解产物中,ORAC、ABTS和DPPH测定的A. occidentale(腰果芽)提取物的抗氧化能力分别为(560504±5785µmol TE/100g DW, 387±0.7 mg TE/g DW和5.9±0.5µg GAE/g DW)。在本研究中,酸性和碱性水解产生了更高的抗氧化能力。结果表明,碱、酸两种水解方式均能使提取物中酚酸类和黄酮类化合物的含量增加,并产生较高的抗氧化能力。
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引用次数: 4
IN-VITRO REGENERATION OF ORTHOSIPHON STAMINEUS (MISAI KUCING) USING AXILLARY BUD 利用腋芽离体再生大麦直立蜂(MISAI KUCING)
Pub Date : 2019-01-07 DOI: 10.26480/gws.01.2019.08.10
Z. Zainuddin, Asmaa' Mohd Kamil
Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique.
直虹吸管是一种受欢迎的草药茶,有助于治疗肾脏和膀胱疾病、糖尿病和痛风。由于对这种药用植物的需求很高,建议大量繁殖这种植物。在本研究中,建立了以腋芽为外植体的正虹吸管雄蕊再生方案。评价了Murashige和Skoog(MS)培养基添加不同浓度的6-苄基氨基嘌呤(BAP)对芽诱导的影响。结果表明,当外植体在添加0.2ppm BAP的MS培养基上培养时,可观察到最佳的射击能力。随后,在含有浓度为0.5ppm和1.0ppm的赤霉素(GA3)的培养基中获得建立的芽的芽伸长。用添加0.5ppm GA3的培养基获得最佳的芽伸长。随后,将伸长的外植体转化到添加0.2ppm 1-萘乙酸(NAA)或0.5ppm NAA的根诱导培养基中。外植体在添加0.2ppm NAA的培养基中表现出阳性反应。通过本研究可以证实,通过组织培养技术,合适浓度的植物生长调节剂可以用于正虹吸管雄蕊的再生。
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引用次数: 1
THE DAMPING LOSS PREVENTION RESEARCH ON AEROBICS SPECIAL SHOE MATERIALS BASED ON INTELLIGENT ANALYSIS 基于智能分析的健美操专用鞋材料防阻尼损耗研究
Pub Date : 2019-01-07 DOI: 10.26480/GWS.01.2019.20.23
S. Qian
{"title":"THE DAMPING LOSS PREVENTION RESEARCH ON AEROBICS SPECIAL SHOE MATERIALS BASED ON INTELLIGENT ANALYSIS","authors":"S. Qian","doi":"10.26480/GWS.01.2019.20.23","DOIUrl":"https://doi.org/10.26480/GWS.01.2019.20.23","url":null,"abstract":"","PeriodicalId":21669,"journal":{"name":"Science Heritage Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46333915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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