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Through the electrodeposition technique, toxic metals in wastewater can be removed and deposited on a chosen substrate with excellent selectivity. In this work, we use this technique to extract lead cations from simulated wastewater by using fluorine-doped tin oxide (FTO) substrate at various temperatures. In situ tracking of lead nucleation at advanced stages has been achieved by chronoamperometry. According to the experimental results, the theoretical models developed to study the kinetic growth of lead deposits in 2D and 3D are in good agreement. Nucleation rate and growth rate constants, for example, were found to be strongly influenced by temperature. Cottrell's equation is used to calculate the diffusion coefficient. X-ray diffraction, scanning electron microscopy, and energy-dispersiveX-ray techniques were used to investigate and characterize the lead deposits. The reported results could provide insight into the optimization of electrodeposition processes for heavy metal recovery from wastewater and electronic wastes.

The influence of vermicompost and various concentrations of lead on the activity of hydrolytic (urease and protease) and oxidative-reducing (catalase and dehydrogenase) enzymes in the sierozem soil of Southern Kazakhstan (Turkestan region) was studied. Background unpolluted soils served as a control. The work shows a change in the enzymatic potential when vermicompost (8 t/ha) and lead are introduced into the soil in the concentration range of 16 to 160 mg/kg Pb. As a result of experimental studies, a decrease in the activities of catalase, protease, and dehydrogenase and, conversely, an increase in the activity of urease with an increase in the lead content in the soil system were revealed. Introduction of vermicompost (vermicompost) into the soil caused an increase in the activity of all studied enzymes and a decrease in the translocation ability of Pb. Inhibition of the process of translocation of lead into plants by vermicompost creates conditions for obtaining environmentally friendly agricultural products.

Pokeweed, Phytolacca americana L., is considered a widely spreading invasive plant, while saponin contents accumulated in the roots have pharmaceutical uses, such as rheumatism treatments and anti-inflammation. Adventitious root cultures are an important source of diverse secondary metabolites, which have significant applications in various fields. This study focused on the optimization of parameters for root induction using different medium states and treatments with auxins on a pokeweed leaf. Semisolid and liquid MS (Murashige and Skoog, 1962) media were supplemented with indole-3-butyric acid (IBA) and 1-naphthylacetic acid (NAA) at 0.5, 1, 2, and 4 mg/L. Root growth parameters, e.g., induction percentage, root numbers, length, and weight, were measured to determine the adventitious root induction efficiency. Total phenolic content, total flavonoid content, total saponin content, and antioxidant activity were recorded. Results showed that adventitious roots induced in semisolid MS medium supplemented with 0.5 mg/L NAA exhibited a high density of lateral roots. Appropriate medium state and auxin for adventitious root induction in pokeweed were determined as semisolid medium supplemented with 2 mg/L NAA. Considering phytochemicals, adventitious roots induced in liquid medium containing 0.5-1 mg/L NAA had the highest yield extract percentage. Additionally, adventitious roots cultivated in a liquid medium enriched with 1 mg/L NAA exhibited the highest phenolic and saponin contents. A principal component analysis (PCA) biplot and hierarchical cluster analysis (HCA) heatmap demonstrated different response patterns between semisolid and liquid media applied with NAA. The results of the semisolid media were grouped together due to high expression levels of the root induction parameters, while elevated phytochemical values were observed in the liquid media treatments. The results suggested two different media that provide the highest adventitious root induction efficiency and the greatest phytochemical contents: semisolid medium with 2 mg/L NAA and liquid medium with 1 mg/L NAA, respectively. These culture media can be applied to optimize adventitious root culture of pokeweed and in vitro phytochemical production.

[This corrects the article DOI: 10.1155/2021/9427978.].

The study was conducted to determine woody plant species composition, structure, and regeneration status in Saleda Yohans Church forest, South Wollo Zone, Ethiopia. Five transect lines oriented to north-south directions at a distance of about 500 m were laid down along transects across the forest. Fifty main plots of 20 m × 20 m were laid for tree/shrub data collection. Within each main plot, 5 small quadrats of 5 m × 5 m were laid in the corners and centre for the collection of seedling and sapling data of woody species. All vegetation data within the plots were counted and recorded. Tree height and diameters at the breast height of plants were also measured and estimated. Moreover, frequency, basal area, diversity, evenness, and other vegetation data were analyzed. The result showed that this Church forest is composed of 50 woody plant species distributed in 31 families. The Shannon-Wiener diversity index and evenness values of the forest were found to be 3.82 and 0.84, respectively. Lamiaceae was the dominant family followed by Fabaceae in their species composition. The density of trees/shrubs, saplings, and seedlings was 625, 650, and 935 ha^-1, respectively. Based on the result, the entire vegetation of Saleda Yohans Church forest is in good regeneration status. In conclusion, although this Church forest appears to be in a good regeneration status, its species richness is low compared to a similar study of some other vegetation. Therefore, attention should be given to the rehabilitation of this forest.

Understanding daily water use determinants is critical to sustainable water access and its efficient use at household level. To pursue this objective, primary data were generated through a survey of 276 respondents across the 5 quarters into which the city of Iwo is divided. 67.5% of the respondents were between 19 and 45 years old while 35.5% were between 46 and 65 years old and the remaining 3.9% comprised of those above 65 years. The results of factor analysis (FA) showed that 12 of the 40 factors analyzed were significant determinants of daily water use in homes. The 12 factors explained 85.794% of the observed variation in household daily water use. The factors in descending order were (i) closeness to water source; (ii) night time baths; (iii) household cooking; (iv) Sunday activities; (v) water demands of the dry season; (vi) morning time water use; (vii) household size; (viii) respondents' attitude; (ix) water availability in the dry season; (x) break in water flow; (xi) social events; and (xii) source of water. Further evaluation condensed the results into four categories, namely, (i) season-associated information; (ii) household-related information; (iii) time of the day; and (iv) water source-related information. The result of correlation analysis showed weak associations among 83.33% of the variables, indicating that each variable should be treated separately from others in the matter relating to daily variation in home water use. The study concluded that household-related information and time of the day (contributing 62.02% of the absolute explanation) are most significant to daily variation in water use at the household level and should be considered when prioritizing effective policies for water use and management.

The production of nonindustrial cannabis is highly developed in the Moroccan Rif region; however, local farmers consider hemp seeds which are rich in omega 3 and tocopherols, only as by-products of cannabis cultivation with low market value. The local ecotype is considered to be a plant with a cannabinoid content of more than 0.4%. So, the objective of this research is to investigate how the incorporation of this local hemp seed affects productive performance and egg quality traits. The experiment is conducted to evaluate the effects of hemp seed (HS) incorporation on hen laying performance and physical egg quality at three levels: 10% (HS-10% group), 20% (HS-20% group), and 30% (HS-30% group). Ninety-six Lohmann Brown classic laying hens were randomly assigned to a control group and three feed treatments. The sampling was taken after the 28-week rearing period (peak egg laying). Throughout the experiment, low-rate HS inclusion (HS-10%) showed no significant differences in egg-laying performance (p > 0.05). However, the high incorporation rates of HS (20% and 30%) negatively affected the egg-laying performance (84-94% and 80-86%, respectively). The albumen quality was also improved by the HS inclusion, where the highest values of the Haugh unit were recorded, ranging between 68.69 and 73.91 for the HS-30% groups. The results also show that HS inclusion and duration influence significantly the yolk color (p < 0.001). The yellow intensity decreases with HS incorporation and aging, from a dark yellow (b ^∗  = 38.63 for the control group) to a very pale yellow (b ^∗  = 26.29 for HS 30% group). Based on these findings, we can conclude that the incorporation of nonindustrial Moroccan cannabis seeds (ecotype Beldiya) at low rate in the diet of laying hens does not alter the laying performance or the quality of the egg; therefore, they could be used in poultry feeding as an alternative constituent to partially replace high-cost imported ingredients, such as corn and soybeans.

Research on the effective attachment of aptamers to beads, which is essential for using aptamers, has made relatively little progress. Here, we demonstrate a new method based on flow cytometry to determine the optimal aptamer-to-bead ratio for aptamer immobilization. The fluorescence intensity increased with a gradual two-fold increase in the aptamer fluorescence concentration, peaked at an aptamer-to-bead ratio of 2.56 × 10⁵, and tended to decrease at higher ratios. A similar pattern was observed in an additional analysis using fluorescence microscopy. However, measurement of the free aptamer concentration after the aptamer-bead conjugation reaction revealed a large aptamer loss compared to the 1.28 × 10⁵ aptamer-bead ratio. In addition, the binding efficiency of the aptamer/bead to the target was highest at the aptamer-to-bead ratio of 1.28 × 10⁵. Taken together, our data suggest that the proposed method is the best and easiest for determining the optimal aptamer-to-bead ratio.

Background: Implantology focuses on the measurement of bone thickness in both the lower and upper jaws. This study aimed to measure and compare alveolar bone thickness of the upper and lower jaws at single edentate sites and cortical bone thickness of their mesial and distal dentate sites.

Methods: Thickness of alveolar bone thickness was measured in 80 upper and 80 lower implant edentate sites and that of buccal and lingual cortical plates of their mesial and distal dentate sites using Cone beam CT. The bone thickness of the edentulous sites was recorded at 3 points (crestal bone, five mm from the crest, and ten mm from the crest), while the bone thickness of the dentate sites was determined at four points (crestal bone, midroot bone, mid of the alveolar bone housing, and apical portion).

Results: An increased amount of bone was measured from the crest to the apical portion of the dentate sites on the buccal and lingual sides of both jaws with a highly significant difference detected among all the tested points (P <  0.0001). No statistical difference was detected between the means of buccal bone width at the first 3 points, except at point 4 (the apical portion), where the mean of the lower jaw (3.35 ± 0.54) was significantly larger than that of the upper jaw (3.17 ± 0.55) (P = 0.04). Bone width measured in the edentulous sites showed a gradual increase from the crest to the apical portions in both jaws.

Conclusion: Bone thickness at the coronal levels is low and susceptible to resorption compared to the apical portions regardless of the dentate state.

In Africa, Alstonia boonei is used folklorically for the management of the multitude of conditions including cataract, which accounts for 50% of cases of blindness in the region. The current study set out to probe the traditional use of the aqueous extract of Alstonia boonei stem bark (ABE) as an anticataract remedy using Sprague Dawley rat models. We investigated the probable phytochemical constituents in the extract, in vitro antioxidant potential, and its in vitro aldose reductase inhibition. For the anticataract investigations, diabetic cataract was induced using galactose in 3-week-old Sprague Dawley rats, and age-related cataract was induced by the administration of sodium selenite to 10-day-old rat pups. Cataract scores in both models were determined after treatment with 30, 100, and 300 mgkg^-1 doses of ABE and 10 mlkg^-1 of distilled water. Lens glutathione, total lens protein, soluble lens proteins (alpha-A) crystallin, and aquaporin 0 levels in the enucleated lens homogenates were determined. Changes in lens to body weight were also determined with histopathological analysis done on the lenses in the selenite-induced cataract model. The presence of alkaloids, tannins, flavonoids, glycosides, and triterpenoids was identified in the extract. The extract inhibited aldose reductase activity with IC₅₀ of 92.30 μgml^-1. The 30, 100, and 300 mgkg^-1ABE-treated rats recorded significantly (p < 0.05) reduced cataract scores indicating a delay in cataractogenesis in galactose-induced cataract and in selenite-induced cataractogenesis as well. Markers of lens transparency such as AQP0, alpha-A crystallin, and total lens proteins and lens glutathione levels were significantly (p < 0.05) preserved. In conclusion, this study establishes the anticataract potential of the aqueous stem bark extract of Alstonia boonei in Sprague Dawley rat models.