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Antibacterial activity of biosynthesized zinc oxide nanoparticles using Kombucha extract 康普茶提取物生物合成氧化锌纳米颗粒的抗菌活性
Q1 Engineering Pub Date : 2023-11-14 DOI: 10.1007/s42452-023-05546-x
Amira A. El-Fallal, Reham A. Elfayoumy, Mohamed M. El-Zahed
Abstract Antibacterial resistance is a growing global vital medical problem when the innovation and development of new antibiotics are dwindling. Different nanomaterials were synthesized and developed as safe and effective alternative antimicrobial agents. The current study highlights the effect of the antibacterial activity of newly biosynthesized zinc oxide nanoparticles (ZnO NPs) obtained from Kombucha extract. Production of ZnO NPs was optimized and the synthesized nanoparticles were characterized using UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Zeta potential and transmission electron microscope (TEM) analysis. The optimum conditions for ZnO NPs production were at temperature 30 °C, pH 9 and 25 mM of Zn(NO 3 ) 2 .6H 2 O. The ZnO NPs biosynthesis was detected in the extract within 24 h. at 35 °C in dark conditions. The XRD pattern displayed 100, 101, 110, and 103 crystal planes. FTIR spectrum showed bands of 1° and 2° amines at 2922.6 cm −1 and a stretching vibration band of vinyl at 1657.5 cm −1 . In addition, the results confirmed the positive charge (19 ± 3 mV) and crystalline nature of spherical-shaped ZnO NPs with an average size of 23 ± 1.5 nm. The antibacterial activity of ZnO NPs was tested against different American-type culture collection strains. ZnO NPs exhibited minimum inhibition concentration (MIC) values of 25 µg/ml against Escherichia coli ATCC25922, 30 µg/ml against Staphylococcus aureus ATCC25923 and Pseudomonas aeruginosa ATCC27853, 35 µg/ml against Serratia liquefaciens OQ071699.1 and S. saprophyticus OQ071703.1 and 40 µg/ml against Lysinibacillus fusiformis OQ071701.1 and Klebsiella pneumoniae ATCC33495. Ultrastructure TEM study of the treated bacteria by MIC of ZnO NPs confirmed their highly toxic action on the bacterial cell wall, resulting in bacterial cell membrane rupture. Treated bacteria appeared as extensively damaged cells with the formation of vacuoles and lipids. The provided approach of ZnO NPs in combination with Kombucha SCOBY has a promising future in overcoming bacterial resistance problems in place of using antibiotics.
摘要随着新型抗生素的创新和开发日益减少,抗菌药物耐药性是一个日益严重的全球性重大医学问题。合成并开发了不同的纳米材料作为安全有效的替代抗菌药物。本研究重点研究了从康普茶提取物中提取的新型生物合成氧化锌纳米颗粒(ZnO NPs)的抗菌活性。优化了ZnO纳米粒子的制备工艺,并利用紫外可见光谱、傅里叶变换红外光谱(FTIR)、x射线衍射(XRD)、Zeta电位和透射电子显微镜(TEM)对合成的纳米粒子进行了表征。制备氧化锌NPs的最佳条件为:温度30℃,pH 9, 25 mM Zn(no3) 2.6 h2o, 35℃,光照条件下,24 h,氧化锌NPs的生物合成完成。XRD图谱显示出100、101、110和103个晶面。FTIR光谱显示在2922.6 cm−1处有1°和2°胺的振动带,在1657.5 cm−1处有乙烯基的伸缩振动带。此外,实验结果还证实了ZnO纳米粒子的正电荷(19±3 mV)和晶体性质,ZnO纳米粒子的平均尺寸为23±1.5 nm。研究了氧化锌NPs对不同美国型培养收集菌株的抑菌活性。氧化锌NPs对大肠杆菌ATCC25922、金黄色葡萄球菌ATCC25923和铜绿假单胞菌ATCC27853、液化沙雷菌OQ071699.1和腐生链球菌OQ071703.1、梭状芽胞杆菌OQ071701.1和肺炎克雷伯菌ATCC33495的最小抑制浓度(MIC)分别为25µg/ml、30µg/ml、40µg/ml。氧化锌NPs的超微结构TEM研究证实了氧化锌NPs对细菌细胞壁的高毒性作用,导致细菌细胞膜破裂。处理过的细菌表现为广泛受损的细胞,形成液泡和脂质。本文提出的氧化锌NPs与康普茶SCOBY联合使用的方法在克服细菌耐药问题方面具有广阔的应用前景。
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引用次数: 0
PV based OFF grid charging station for E-vehicles using PWM and phase shift controlled interleaved three port converter 基于PWM和相移控制交错三端口变换器的电动汽车离网充电站
Q1 Engineering Pub Date : 2023-11-14 DOI: 10.1007/s42452-023-05571-w
Kudiyarasan Swamynathan, N. Mahalingam, Alagumariyappan Paramasivam, Sankaran Vijayalakshmi
Abstract In recent years, Electric Vehicles are becoming more popular. The pollution level in the atmosphere can be effectively minimized by using Electric vehicles for large-scale transportation. A battery station is required for continuous operation; however, the Photovoltaic-based OFF grid charging station can only operate during the day. Therefore, the three-port converters have started to arise from a number of current EV charging station developments. In this study, a unique PWM and Phase Shift Controller are proposed to reduce switching losses and to improve reliability. In addition, for Maximum PowerPoint Tracking, a Fuzzy is added to the PV system. Furthermore, an appropriate interleaved boost converter topology is used to create the various charging voltages required for EV and battery stations. The proposed topology is simulated, and the hardware prototype has been created and tested. The result shows that the proposed topology has a better efficiency than the traditional converters. Article Highlights. A dual composite charging station for electric vehicle charging in environment friendly manner. Optimization of power electronics required in Electric Vehicle charging stations. Maximum utilization of battery for cyclic charging from charger and discharging through Electric Vehicles.
近年来,电动汽车越来越受欢迎。使用电动汽车进行大规模运输可以有效地降低大气中的污染水平。需要一个电池站来连续运行;然而,基于光伏的OFF电网充电站只能在白天运行。因此,三端口转换器已经开始出现在一些当前的电动汽车充电站的发展。在本研究中,提出了一种独特的PWM和相移控制器,以减少开关损耗并提高可靠性。此外,对于最大的ppt跟踪,一个模糊被添加到光伏系统。此外,采用适当的交错升压变换器拓扑来产生电动汽车和电池站所需的各种充电电压。对所提出的拓扑结构进行了仿真,并创建了硬件原型并进行了测试。结果表明,该拓扑结构比传统的变换器具有更高的效率。文章集锦。一种用于电动汽车环保充电的双复合充电站。电动汽车充电站电力电子系统优化。最大限度地利用电池,通过电动汽车从充电器循环充电和放电。
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引用次数: 0
Correction to: A scoping review on drone technology applications in forensic science 对无人机技术在法医科学中的应用进行了范围审查
Q1 Engineering Pub Date : 2023-11-13 DOI: 10.1007/s42452-023-05573-8
Natassya Ezzaty Mohd Sabri, Mansharan Kaur Chainchel Singh, Mohd Shah Mahmood, Lay See Khoo, Mohd Yusmiaidil Putera Mohd Yusof, Chong Chin Heo, Muhammad Dzulfiqri Muhammad Nasir, Hapizah Nawawi
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引用次数: 0
Correction to: Boosting the nonlinear optical absorption of graphene oxide, and gold nanorods by tailoring graphene oxide-gold nanorods hybrids 修正:通过定制氧化石墨烯-金纳米棒的杂交体,提高氧化石墨烯和金纳米棒的非线性光学吸收
Q1 Engineering Pub Date : 2023-11-13 DOI: 10.1007/s42452-023-05542-1
Abeer Salah, Salah Hassab-Elnaby, Marwa A. Ramadan
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引用次数: 0
Towards efficient video-based action recognition: context-aware memory attention network 基于视频的高效动作识别:情境感知记忆注意网络
Q1 Engineering Pub Date : 2023-11-13 DOI: 10.1007/s42452-023-05568-5
Thean Chun Koh, Chai Kiat Yeo, Xuan Jing, Sunil Sivadas
Abstract Given the prevalence of surveillance cameras in our daily lives, human action recognition from videos holds significant practical applications. A persistent challenge in this field is to develop more efficient models capable of real-time recognition with high accuracy for widespread implementation. In this research paper, we introduce a novel human action recognition model named Context-Aware Memory Attention Network (CAMA-Net), which eliminates the need for optical flow extraction and 3D convolution which are computationally intensive. By removing these components, CAMA-Net achieves superior efficiency compared to many existing approaches in terms of computation efficiency. A pivotal component of CAMA-Net is the Context-Aware Memory Attention Module, an attention module that computes the relevance score between key-value pairs obtained from the 2D ResNet backbone. This process establishes correspondences between video frames. To validate our method, we conduct experiments on four well-known action recognition datasets: ActivityNet, Diving48, HMDB51 and UCF101. The experimental results convincingly demonstrate the effectiveness of our proposed model, surpassing the performance of existing 2D-CNN based baseline models. Article Highlights Recent human action recognition models are not yet ready for practical applications due to high computation needs. We propose a 2D CNN-based human action recognition method to reduce the computation load. The proposed method achieves competitive performance compared to most SOTA 2D CNN-based methods on public datasets.
鉴于监控摄像机在我们日常生活中的普遍存在,从视频中识别人类行为具有重要的实际应用价值。该领域的一个持续挑战是开发更有效的模型,能够实时识别和高精度的广泛实施。本文提出了一种新的人体动作识别模型——情境感知记忆注意网络(CAMA-Net),该模型消除了计算量大的光流提取和三维卷积。通过去除这些组件,CAMA-Net在计算效率方面比许多现有方法具有更高的效率。CAMA-Net的一个关键组件是上下文感知记忆注意模块,该注意模块计算从2D ResNet主干获得的键值对之间的相关性评分。这个过程建立了视频帧之间的对应关系。为了验证我们的方法,我们在四个知名的动作识别数据集上进行了实验:ActivityNet, Diving48, HMDB51和UCF101。实验结果令人信服地证明了我们提出的模型的有效性,超越了现有的基于2D-CNN的基线模型的性能。由于计算量大,目前的人体动作识别模型还没有做好实际应用的准备。为了减少计算量,提出了一种基于二维cnn的人体动作识别方法。与大多数基于SOTA 2D cnn的方法相比,该方法在公共数据集上取得了具有竞争力的性能。
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引用次数: 0
Analysis of torque controlling strategies of interior permanent magnet synchronous machine in hybrid electric vehicle 混合动力汽车内嵌式永磁同步电机转矩控制策略分析
Q1 Engineering Pub Date : 2023-11-11 DOI: 10.1007/s42452-023-05563-w
S. Usha, P. Geetha, R. Palanisamy, None Kitmo, Yosef Berhan Jember
Abstract Significance of this research is based on torque controlling strategies in hybrid electric vehicle. The traction motor of a hybrid electric commercial vehicle must have a high power density-to-weight ratio and excellent torque at extremely low speeds. A proportional-integral controller estimates the high-resolution rotor position based on the difference between actual as well as reference output power. This technique can correct for hall position sensor installation error and also accurately estimate rotor position. In this study, a current proportional integral controller is also used to determine the permanent magnetic flux linkage. Experiments are used to test other key metrics such as d-axis and q-axis inductances, stator resistance, and energy loss. The measured parameters are kept in lookup tables that span the whole operating range at various current levels. A maximum torque per ampere control methodology, paired with the feed forward parameter iteration method, may be used to produce accurate and efficient torque control based on these precise parameters. The standard driving cycle performance requirements of the modelled Federal Test Procedure vehicle is analysed in the article for understanding the real model requirements of the vehicle. The efficacy of the proposed methodology using Matlab/Simulink environment is analysed. Both modelling and experimental findings validate the efficacy, torque ripple, stator and rotor flux, sensitivity of the vehicle parameter as well as the computational complexity of the proposed methodology.
摘要本文研究的意义在于混合动力汽车的转矩控制策略。混合动力商用车的牵引电机必须具有较高的功率密度重量比和极低速度下的优异扭矩。比例积分控制器根据实际输出功率和参考输出功率之间的差估计高分辨率转子位置。该方法既能校正霍尔传感器的安装误差,又能准确估计转子位置。在本研究中,还使用电流比例积分控制器来确定永磁通链。实验用于测试其他关键指标,如d轴和q轴电感,定子电阻和能量损耗。测量参数保存在查找表中,该表涵盖了在不同电流水平下的整个工作范围。最大转矩/安培控制方法与前馈参数迭代法相结合,可以在这些精确参数的基础上实现准确有效的转矩控制。本文对模拟的联邦试验程序车辆的标准行驶工况性能要求进行了分析,以了解车辆的真实模型要求。在Matlab/Simulink环境下,分析了该方法的有效性。模型和实验结果验证了该方法的有效性、转矩脉动、定子和转子磁链、车辆参数的灵敏度以及计算复杂度。
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引用次数: 0
Soil improvement to enhance resistance parameters using bacterial precipitation and nanosilica 利用细菌沉淀和纳米二氧化硅改良土壤以提高抗性参数
Q1 Engineering Pub Date : 2023-11-11 DOI: 10.1007/s42452-023-05551-0
Moein Khoshdel Sangdeh, Alireza Negahdar, Fatemeh Tabandeh
Abstract Soil has an important role in the structure of every building. Although there are different methods available to improve the mechanical properties of soil (adhesion, friction, and rigidity), some, such as chemical methods, despite their limited advantages, will harm the environment. The present study examined the use of microbiologically induced calcite precipitation, which is good for the environment and is a stable improvement method. An experimental study was conducted to study the influence of different factors on the resistance parameters of improved sandy soil. These parameters were the concentration of reactive materials (0.25, 0.5, 0.75 M), curing time (3, 7, 14 days), and compatibility of the bacteria with the nanoparticle. The results showed that the shear strength parameters improved and the soil adhesion rate increased from 0.1 to 186. The soil friction angle reached 35.6°, indicating a 12% increase compared to that of unimproved sand.
土在任何建筑的结构中都起着重要的作用。虽然有不同的方法可以改善土壤的机械特性(附着力、摩擦力和刚性),但有些方法,如化学方法,尽管优势有限,但会损害环境。本研究探讨了利用微生物诱导方解石沉淀对环境有益,是一种稳定的改善方法。通过试验研究了不同因素对改良沙土抗蚀性参数的影响。这些参数是反应物质的浓度(0.25,0.5,0.75 M),固化时间(3,7,14天),以及细菌与纳米颗粒的相容性。结果表明:土体抗剪强度参数得到改善,黏附率由0.1提高到186;土摩擦角达到35.6°,比未改良砂增大12%。
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引用次数: 0
Design of a polymeric cerebral aneurysm based on numerical modelling for the development of an aneurysm mechanical characterisation device 基于数值模拟的聚合物脑动脉瘤的设计,用于动脉瘤机械表征装置的开发
Q1 Engineering Pub Date : 2023-11-10 DOI: 10.1007/s42452-023-05553-y
Jolan Raviol, Guillaume Plet, Hélène Magoariec, Cyril Pailler-Mattei
Abstract Intracranial aneurysm is a life-threatening pathology related to the arterial wall alteration. As yet there is no method capable of predicting rupture risk based on quantitative in vivo mechanical data. This work is part of a large-scale project aimed at providing clinicians with a non-invasive patient-specific decision support tool, based on the in vivo mechanical characterisation of the aneurysm wall. First, an original wall deformation device was developed on polymeric phantom arteries. These artery models were obtained by 3D printing and an injection moulding process, each one showing pros and cons of designs of a biofidelic phantom in terms of thickness and local stiffness. A numerical modelling of this experimental study was built as a support for designing phantoms as design process choices and determining the geometrical and mechanical parameters of arteries. A numerical Fluid–Structure Interaction model based on the finite element method was developed. Several wall thicknesses, mechanical properties and deformation device locations were considered. Regarding the 3D printed phantom artery, the numerical model demonstrated that a thin wall thickness should be emphasised instead of a low Young’s modulus to reach a significant and experimentally observable strain. Regarding the injection moulded phantom, the results pointed to a locally reduced aneurysm thickness with a Young’s modulus of 0.7 MPa for the strain analysis. The numerical study provided helpful information regarding the scientific challenges of the experimental study. This work is the keystone of further animal studies and associated patient-specific models.
颅内动脉瘤是一种与动脉壁改变有关的危及生命的病理。到目前为止,还没有一种方法能够基于定量的体内力学数据来预测破裂风险。这项工作是一个大型项目的一部分,旨在为临床医生提供一种基于动脉瘤壁体内力学特征的非侵入性患者特异性决策支持工具。首先,在聚合物幻动脉上研制了一种原始的壁变形装置。这些动脉模型是通过3D打印和注射成型工艺获得的,每个模型在厚度和局部刚度方面都显示了生物神经模型设计的优点和缺点。建立了本实验研究的数值模型,作为设计工艺选择和确定动脉几何和力学参数的支撑。建立了基于有限元法的流固耦合数值模型。考虑了不同的壁厚、力学性能和变形装置的位置。对于3D打印的幻动脉,数值模型表明,应该强调薄壁厚度,而不是低杨氏模量,以达到显著的和实验可观察到的应变。对于注射模体,结果表明局部动脉瘤厚度减小,杨氏模量为0.7 MPa,用于应变分析。数值研究为实验研究的科学挑战提供了有用的信息。这项工作是进一步的动物研究和相关的患者特异性模型的基石。
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引用次数: 0
Life cycle assessment of the casting process with the addition of niobium from scrap 废铌加入铸造工艺的生命周期评价
Q1 Engineering Pub Date : 2023-11-10 DOI: 10.1007/s42452-023-05569-4
Henrique G. da Silva, Joao C. E. Ferreira, Tamile Kelm, Thiago M. Ivaniski
Abstract Sustainability has become increasingly important, given the scenario of environmental degradation, and the industrial sector is significantly responsible for this adverse scenario. An important manufacturing process is casting, but it consumes many natural resources and generates waste, emitting pollutants and consuming much water and electricity. Thus, this work investigated the environmental impacts through life cycle assessment (LCA) arising from the gray cast iron casting process by adding niobium from scrap. Niobium improves molten material’s structure and mechanical properties, and it has been obtained from a company that separates it with a ballistic magnetic separator. A production model of 800 ingots was devised, using recycled material from a supplier and reused material from the factory itself, such as recycled sand. The processes using recycled materials resulted in lower impacts than those using new materials. Sand recycling and sand mold preparation stages have significant impact due to the presence of binders, typically used in sand molds, and transportation by vehicles powered by fossil fuels. Abiotic depletion indicates the need to transition to renewable energy sources and sustainable materials, such as developing clean energy infrastructure. Regarding global warming, there were high values for all stages of the casting process, with the highest value in the sand recycling stage. To prevent eutrophication, proper waste management should be implemented, such as cleaner technologies in the smelter implementing efficient effluent treatment systems. Concerning freshwater ecotoxicity, the effluents generated should be treated to reduce the concentration of pollutants before being discarded in the aquatic environment.
考虑到环境恶化的情况,可持续发展变得越来越重要,而工业部门对这种不利情况负有重大责任。铸造是一个重要的制造过程,但它消耗许多自然资源,产生废物,排放污染物,消耗大量的水和电。因此,本工作通过生命周期评估(LCA)研究了从废料中添加铌的灰铸铁铸造工艺对环境的影响。铌改善了熔融材料的结构和力学性能,并从一家公司用弹道磁选机分离它。设计了一个800锭的生产模型,使用供应商的回收材料和工厂自己的再利用材料,如回收的沙子。与使用新材料的工艺相比,使用回收材料的工艺产生的影响更小。由于砂模中通常使用粘合剂,以及化石燃料驱动的交通工具的存在,砂的回收和砂模制备阶段会产生重大影响。非生物耗竭表明需要向可再生能源和可持续材料过渡,例如发展清洁能源基础设施。对于全球变暖,铸造过程的各个阶段都有较高的数值,其中砂回收阶段的数值最高。为了防止富营养化,应实施适当的废物管理,例如在冶炼厂采用更清洁的技术,实施有效的污水处理系统。在淡水生态毒性方面,对产生的废水进行处理,降低污染物浓度,然后排入水生环境。
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引用次数: 0
Fractional-view analysis of the transmission dynamics of a bacterial infection with nonlocal and nonsingular kernel 非局部非奇异核细菌感染传播动力学的分式分析
Q1 Engineering Pub Date : 2023-11-10 DOI: 10.1007/s42452-023-05538-x
Hakima Degaichia, Rashid Jan, Ziad Ur Rehman, Salah Boulaaras, Asif Jan
Abstract An enormous cost is placed on people, communities, and healthcare systems by bacterial infections. Measures of the burden of bacterial infections include morbidity, mortality, economic expenditures, and overall effects on public health. Campylobacteriosis is a bacterial infection imposes a significant economic burden on both individuals and societies due to its prevalence, healthcare costs, and the associated loss of productivity. In our research, we develop a model to analyze the transmission of campylobacteriosis infection, taking into account factors such as vaccination and treatment. We also examine the fundamental characteristics of fractional calculus to understand the model better. The equilibria of the model are studied, and we calculate the reproduction parameter denoted as $$mathcal{R}_{0}$$ R 0 . Furthermore, we provide proof of stability for the equilibria of the system. Lastly, we conduct numerical investigations to explore the variation of the system’s reproduction parameter with different input parameters. We have established the necessary conditions to ensure the existence and uniqueness of solutions for the proposed model of campylobacteriosis infection. To better understand the complex dynamics of campylobacteriosis infection, we conduct various simulations of the suggested model while modifying the input factors. These simulations allow us to investigate the effects of different input parameters on the dynamics of campylobacteriosis infection. We analyze the dynamic behavior of the system to develop efficient control strategies for managing the infection. Notable improvements have been observed by reducing the order of the fractional derivative. Based on our findings, we propose various factors to the policy makers in the community to mitigate the spread of campylobacteriosis infection.
细菌感染给人们、社区和医疗保健系统带来了巨大的成本。衡量细菌感染负担的措施包括发病率、死亡率、经济支出和对公共卫生的总体影响。弯曲杆菌病是一种细菌感染,由于其普遍性、医疗费用和相关的生产力损失,给个人和社会带来了重大的经济负担。在我们的研究中,我们建立了一个模型来分析弯曲杆菌病感染的传播,考虑到疫苗接种和治疗等因素。我们还考察了分数阶微积分的基本特征,以便更好地理解该模型。研究了模型的均衡,并计算了模型的再生参数$$mathcal{R}_{0}$$ R 0。进一步给出了系统平衡点的稳定性证明。最后,通过数值研究探讨了不同输入参数下系统再生参数的变化规律。我们已经建立了必要的条件,以确保所提出的弯曲杆菌感染模型解的存在性和唯一性。为了更好地理解弯曲杆菌感染的复杂动力学,我们在修改输入因素的同时对所建议的模型进行了各种模拟。这些模拟使我们能够研究不同输入参数对弯曲杆菌病感染动力学的影响。我们分析了系统的动态行为,以制定有效的控制策略来管理感染。通过降低分数阶导数的阶数,可以观察到显著的改进。基于我们的研究结果,我们向社区决策者提出了各种因素来减轻弯曲杆菌病感染的传播。
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引用次数: 0
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