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Staining of different tissues in thick epoxy resin-impregnated sections of human fetuses. 人类胎儿厚环氧树脂浸渍切片中不同组织的染色。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108049
H Fritsch

Sections of undecalcified human fetuses, fixed in formaldehyde, embedded in the epoxy resin Biodur E 12 and cut on a diamond-wire saw were stained according to a slight modification of the method described by Laczkó and Lévai. The sections were immersed in a methylene blue/azure II solution at 90 C for at least 3 min and counterstained with a basic fuchsin solution at the same temperature. Differential staining was as follows: bone stained pinkish; cartilage, violet; collagen fibers, blue-violet; elastic fibers, red and muscle fibers, green-blue. Most other tissues were stained blue-violet against the transparent background of the embedding epoxy resin. Thanks to the distinct and differential staining of each tissue, contrast is sufficient for black and white as well as for color photography.

未钙化的人类胎儿切片,固定在甲醛中,包埋在环氧树脂Biodur E 12中,在金刚石线锯上切割,根据Laczkó和l录影带描述的方法进行轻微修改。切片在90℃的亚甲基蓝/天蓝II溶液中浸泡至少3分钟,并在相同温度下用碱性品红溶液反染色。鉴别染色如下:骨染粉红色;软骨,紫色;胶原纤维,蓝紫色;弹力纤维,红色肌肉纤维,绿蓝色。大多数其他组织在包埋环氧树脂的透明背景下被染成蓝紫色。由于不同组织的不同染色,对比度足以用于黑白摄影和彩色摄影。
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引用次数: 73
A new, rapid, microwave-stimulated method of staining melanocytic lesions. 一种新的、快速的、微波刺激的黑素细胞病变染色方法。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108050
A S Leong, P Gilham

A new and sensitive method of staining melanocytic lesions is described. Tissue sections covered by a solution of colloidal silver nitrate are exposed to microwaves for 45 sec in a domestic oven to produce clean, crisp staining of melanocytes and melanoma cells, often showing long delicate dendritic cell processes. The staining technique does not stain other pigments or argyrophilic tissues and is shown to be more sensitive than the standard Masson-Fontana procedure.

描述了一种新的、灵敏的黑素细胞病变染色方法。用硝酸银胶体溶液覆盖的组织切片在家用烤箱中微波45秒,产生干净、清晰的黑素细胞和黑色素瘤细胞染色,通常显示出长而精致的树突状细胞过程。该染色技术不会染色其他色素或亲银组织,并被证明比标准Masson-Fontana程序更敏感。
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引用次数: 21
Improving stain penetration into epoxy resin sections for light microscopy. 改善染色渗透到环氧树脂切片光学显微镜。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108054
H H Crowley
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引用次数: 2
A high pH discontinuous buffer system for resolution of isozymes in starch gel electrophoresis. 淀粉凝胶电泳中同工酶的高pH不连续缓冲系统。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108047
P Chippindale

A Tris-citrate pH 9.5 gel/borate pH 8.2 electrode discontinuous buffer system for starch gel electrophoresis of proteins was developed to resolve iso- and allozymes of aspartate aminotransferase in frogs (Hyla crucifer). This buffer system also enhanced resolution of NADP-dependent malate dehydrogenase and the L-lactate dehydrogenase-A locus in this species. It provided good resolution of NAD-dependent malate dehydrogenase in esocid fishes, and esterases, glycerol-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, and S-aconitate hydratase in ambystomatid salamanders. Variation suppressed by other buffers was revealed by this buffer for some enzyme encoding loci, while at other loci, this buffer suppressed electromorph variability. The concentration of tris(hydroxymethyl)aminomethane in gels made with this buffer was much higher than in pH 8.7 "Poulik" gels, but running characteristics of the two gel types were similar. Gels made with this new buffer were less prone to splitting and "warping" than Poulik gels, and were easier to handle. When screening a given taxon for enzyme variability, tests using multiple buffers are essential to maximize the amount of electrophoretically detectable variation.

建立了一种pH值为9.5的三柠檬酸盐凝胶/ pH值为8.2的硼酸盐电极不连续缓冲系统,用于蛋白质淀粉凝胶电泳,用于分离青蛙(Hyla crucifer)的天冬氨酸转氨酶异酶和同酶。该缓冲系统还提高了该物种nadp依赖性苹果酸脱氢酶和l -乳酸脱氢酶a位点的分辨率。该方法能很好地分离鱼类中nadd依赖性苹果酸脱氢酶,以及ambystomatid salamander中酯酶、甘油-3-磷酸脱氢酶、甘油醛-3-磷酸脱氢酶、醇脱氢酶和S-aconitate水合酶。在某些酶编码位点上,这种缓冲液抑制了其他缓冲液的变异,而在其他位点上,这种缓冲液抑制了电形态的变异。用该缓冲液制备的凝胶中三(羟甲基)氨基甲烷的浓度远高于pH为8.7的“Poulik”凝胶,但两种凝胶的运行特性相似。与Poulik凝胶相比,用这种新缓冲液制成的凝胶更不容易分裂和“翘曲”,而且更容易处理。当筛选一个给定的分类单元的酶变异性时,使用多个缓冲液的测试是必不可少的,以最大限度地提高电泳可检测的变异量。
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引用次数: 2
Paraffin sections of nervous tissue supravitally stained with methylene blue: a new, reliable and simple fixation technique. 亚甲基蓝染色神经组织上石蜡切片:一种新的、可靠的、简单的固定技术。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108052
T Müller

Two steps are recommended for fixation of central nervous tissue after supravital staining with methylene blue: application of ammonium heptamolybdate followed by a paraformaldehyde-glutaraldehyde mixture. The two fixatives complement each other so that both dye and tissue are optimally fixed. Molybdate renders the dye water and alcohol insoluble, the aldehyde mixture conditions the tissue for embedding in paraffin. This technique is a suitable alternative to conventional whole mount and frozen sectioning methods.

亚甲基蓝染色后,中枢神经组织的固定推荐两个步骤:应用七钼酸铵,然后是多聚甲醛-戊二醛混合物。这两种固定剂相互补充,使染料和组织都得到最佳固定。钼酸盐使染料不溶于水和酒精,乙醛混合物使组织包埋在石蜡中。该技术是传统的整片和冷冻切片方法的合适替代方法。
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引用次数: 3
Method for the preservation of polystyrene latex beads in tissue sections. 组织切片中聚苯乙烯乳胶珠的保存方法。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108055
M Callebaut, C Meeussen
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引用次数: 9
A simplified aluminum stain in paraffin sections of bone from hemodialysis patients. 血液透析患者骨石蜡切片的简化铝染色。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108046
Y Ohtsuki, T Yamaguchi, H Sonobe, K Takahashi, K Hayashi, A Takenaka, H Hashimoto, K Kuwabara, T Miyamoto, N Terao

A new simplified method has been devised for staining aluminum and has been tested in paraffin sections of bone from 60 patients who have undergone hemodialysis. Iliac crest bone fragments were fixed in 20% phosphate-buffered formalin for less than a day and demineralized at room temperature in 10% phosphate-buffered formalin containing 5% formic acid for only 2 to 3 hr. Four-micron paraffin sections, accompanied by positive controls, were stained with Maloney's aluminum stain, the Berlin blue reaction for iron, dylon or Congo red for amyloid and von Kossa's reaction for calcium. Aluminum and iron were demonstrated particularly at the mineralizing front of bony tissues; aluminum in 52 cases, iron in 45. Dylon staining also gave positive results in 52 cases. It is important in determining whether aluminum deposition is present that the von Kossa reaction remains positive even after demineralization. This method may be more useful for demonstrating aluminum in bony tissues than the complicated and time-consuming resin-embedding method currently used.

一种新的简化方法被设计出来用于铝染色,并在60名接受过血液透析的患者的骨石蜡切片上进行了测试。髂嵴骨碎片在20%磷酸盐缓冲福尔马林中固定不到一天,在室温下在含有5%甲酸的10%磷酸盐缓冲福尔马林中脱矿仅2至3小时。4微米石蜡切片,并附有阳性对照,用马洛尼铝染色,柏林蓝反应铁染色,涤纶或刚果红反应淀粉样蛋白染色,冯科萨反应钙染色。铝和铁在骨组织的矿化前沿表现得尤为明显;52箱铝,45箱铁。Dylon染色也有52例阳性。von Kossa反应即使在脱矿后仍保持阳性,这对于确定铝沉积是否存在是很重要的。该方法可能比目前使用的复杂且耗时的树脂包埋方法更有助于证明骨组织中的铝。
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引用次数: 11
Activated charcoal as a adsorbent of oxidized 3,3'-diaminobenzidine in peroxidase cytochemistry. 活性炭在过氧化物酶细胞化学中对氧化3,3′-二氨基联苯胺的吸附剂。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108053
A. Ros Barceló, R. Muñoz, F. Sabater
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引用次数: 5
Transmission electron microscopic study of maize pachytene chromosome 6. 玉米粗线体6号染色体的透射电镜研究。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108048
D L Shen, M Wu

Cytoplasm-free chromosomes are frequently obtained in meiotic chromosome spreads prepared from mildly-fixed maize microsporocytes. These chromosomes are suitable for detailed structural analysis using a published electron microscopic technique. In the electron micrograph, the knobs and heterochromatin regions that have been used for karyotype analyses in the light microscope are clearly visible. Therefore, the electron microscopic map can be easily aligned with the traditional cytological map. In addition to these prominent structural features, numerous electron-dense bands also are observed. To determine whether the bands can be used as markers for the identification of each chromosomal subregion, the banding pattern of chromosome 6 is analyzed. Chromosome 6 is frequently associated with the nucleolus and can be easily recognized. We observed that at the zygotene stage in prophase I, electron-dense regions are detected on each homolog of the synapsing chromosome. During synapsis, the electron-dense regions on both homologs are brought into register to form more conspicuous bands. At the early pachytene stage, the banding pattern is stable and reproducible. Chromosome 6 contains eight dark bands, 19 medium bands and 14 light bands. The bands can be used as intrachromosomal markers for regional assignment of genes in detailed in situ hybridization mapping or cytogenetic studies. As the pachytene stage progresses, condensation of the chromosome bivalents is accompanied by fusion of adjacent bands.

从温和固定的玉米小孢子细胞制备的减数分裂染色体扩增中经常获得无细胞质染色体。这些染色体适合使用已发表的电子显微镜技术进行详细的结构分析。在电子显微镜下,光镜下用于核型分析的旋钮和异染色质区域清晰可见。因此,电子显微镜图谱可以很容易地与传统的细胞学图谱对齐。除了这些突出的结构特征外,还观察到许多电子密带。为了确定条带是否可以作为各染色体亚区鉴定的标记,对6号染色体的条带模式进行了分析。6号染色体通常与核仁相关,很容易识别。我们观察到,在I前期的合子蛋白阶段,在突触染色体的每一个同源物上都检测到电子密集区。在突触过程中,两个同源物上的电子密集区域被带入寄存器,形成更明显的带。在粗线期早期,条带模式是稳定的和可复制的。6号染色体包含8条暗带、19条中带和14条亮带。这些条带可以用作染色体内标记,用于详细的原位杂交作图或细胞遗传学研究中基因的区域分配。随着粗线期的进展,染色体二价体的凝聚伴随着相邻条带的融合。
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引用次数: 6
Activated charcoal as a adsorbent of oxidized 3,3'-diaminobenzidine in peroxidase cytochemistry. 活性炭在过氧化物酶细胞化学中对氧化3,3′-二氨基联苯胺的吸附剂。
Pub Date : 1989-03-01
A Ros Barceló, R Muñoz, F Sabater
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引用次数: 0
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Stain technology
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