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A new method for demonstrating argyrophil cells of the pancreas and intestines. 胰、肠嗜银细胞的一种新方法。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108051
W Garvey, A Fathi, F Bigelow, B Carpenter, C Jimenez

A new method for demonstrating argyrophil cells of the pancreas and intestinal tract using a combined silver and reducing solution in sections of formaldehyde fixed tissue is described. Impregnating sections in a 60 C water bath, the procedure takes about 25 min. A microwave version that takes about 5 min is also given. Results are similar to those obtained with the Grimelius method for argyrophil cells.

介绍了一种用银和还原溶液在甲醛固定组织切片上显示胰腺和肠道嗜银细胞的新方法。在60摄氏度的水浴中浸渍切片,过程大约需要25分钟。也提供了一个大约需要5分钟的微波版本。结果与用Grimelius法测定嗜银细胞的结果相似。
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引用次数: 6
A new infusion needle for perfusion fixation. 一种新型灌注固定输液针制造技术。
Pub Date : 1989-03-01 DOI: 10.3109/10520298909108056
J Hiraoka, W Wang
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引用次数: 0
Staining of developing neurites with coomassie blue. 发育中的神经突用考马斯蓝染色。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108044
N G Carri, T Ebendal
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引用次数: 2
Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections. 组织切片中皮肤硫酸酯蛋白多糖的免疫组织化学检测技术。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108042
M Sobue, J Takeuchi, T Fukatsu, T Nagasaka, N Nakashima, T Ogura, T Katoh, K Yoshida

Dermatan sulfate proteoglycan chains were detected in tissue sections treated with chondroitin B-lyase (0.01 units/ml) in 20 mM Tris-HCl (pH 8.0) for 1 hr, followed by staining with antibody 9A2 specific for unsaturated uronic acid coupled to N-acetylgalactosamine-4 sulfate. In contrast, after treatment with chondroitin B-lyase, no positive staining was observed with antibodies 3B3 and 1B5 which react to the unsaturated uronic acid coupled to N-acetylgalactosamine 6-sulfate and unsaturated uronic acid coupled to N-acetylgalactosamine, respectively. The distribution of dermatan sulfate thus revealed was confirmed by comparison with that found by monoclonal antibody 6B6 which reacts with small proteoglycans carrying dermatan sulfate side chains. The localization of positive staining in fibrous connective tissues was almost identical with these two procedures.

用软骨素b -裂解酶(0.01单位/ml)在20 mM Tris-HCl (pH 8.0)中处理1小时的组织切片检测硫酸皮肤聚糖蛋白多糖链,然后用n -乙酰半乳糖胺-4硫酸盐偶联不饱和脲酸特异性抗体9A2染色。与此相反,经软骨素b -解酶处理后,抗体3B3和1B5分别与n -乙酰半乳糖胺6-硫酸盐偶联的不饱和脲酸和n -乙酰半乳糖胺偶联的不饱和脲酸反应,未见阳性染色。通过与携带硫酸皮聚糖侧链的小蛋白聚糖反应的单克隆抗体6B6的对比,证实了硫酸皮聚糖的分布。纤维结缔组织阳性染色的定位与这两种方法几乎相同。
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引用次数: 17
A simple procedure to obtain one-micrometer sections of routinely embedded paraffin material. 一个简单的程序,以获得一微米切片常规包埋石蜡材料。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108041
L C Junqueira, M D Silva, H Torloni

By freezing blocks of paraffin-embedded tissues to a convenient temperature it is possible to obtain routinely 1 micron sections that can be further processed as normal thicker sections. Normal and disposable steel knives can be used and the staining time should be increased in most procedures. Gradual freezing of blocks to the temperature of dry ice is the simplest and safest way to obtain an adequate temperature. The best results were obtained using as fixative 4% paraformaldehyde in phosphate buffered saline solution.

通过将石蜡包埋的组织块冷冻到方便的温度,可以获得常规的1微米切片,可以进一步处理为正常的较厚切片。可使用普通和一次性钢刀,大多数程序应增加染色时间。将冰块逐渐冻结到干冰的温度是获得适当温度的最简单和最安全的方法。以4%多聚甲醛为固定剂,在磷酸盐缓冲盐水溶液中固定效果最好。
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引用次数: 4
Histological preparation of implanted biomaterials for light microscopic evaluation of the implant-tissue interaction. 植入生物材料的组织学准备,用于植入物与组织相互作用的光镜评估。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108039
E Murice-Lambert, A B Banford, R L Folger

A technique is presented for processing implanted biomaterials with surrounding soft tissue for histological assessment of the implant-tissue interaction. Specimens are removed with the implant-tissue interface intact, fixed in formalin, dehydrated in a graded series of ethanol followed by a graded series of acetone in ethanol, and embedded in Spurr's low viscosity epoxy resin. Sections 0.5-1.0 mm thick are cut from the cured blocks using a metallurigical saw with a diamond wafer blade. After being glued to glass microscope slides, they are ground and polished to approximately 75 microns in thickness. The polished sections are treated with 95% ethanol saturated with sodium hydroxide, stained with Gill's hematoxylin and counterstained in eosin Y-phloxine B. The sodium hydroxide solution degrades the resin, allowing the stain to penetrate the tissue. By limiting the time in sodium hydroxide, the depth of staining is controlled and one is able to simulate a thin paraffin section with high resolution of the implant-soft tissue interface.

提出了一种处理植入生物材料与周围软组织的技术,用于植入物与组织相互作用的组织学评估。标本在植入物-组织界面完整的情况下取出,在福尔马林中固定,在分级系列乙醇中脱水,然后在乙醇中分级系列丙酮中脱水,然后嵌入spr的低粘度环氧树脂中。用带金刚石片刀片的冶金锯从固化块上切下0.5-1.0毫米厚的部分。在被粘到玻璃显微镜载玻片上后,它们被研磨和抛光到大约75微米的厚度。抛光后的切片用饱和氢氧化钠的95%乙醇处理,用吉尔苏木精染色,并用伊红y -苯氧辛b反染色。氢氧化钠溶液降解树脂,使染色渗入组织。通过限制在氢氧化钠中的时间,可以控制染色深度,并且可以模拟具有高分辨率的种植体-软组织界面的薄石蜡切片。
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引用次数: 28
A new technique for staining catecholic residues in biological samples. 生物样品中儿茶酚残留染色新技术。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108038
R Kalyani, K Nellaiappan

This technique for localizing catecholic residues in biological samples is based on the condensation of Besthorn's hydrazone (3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) with quinone residues obtained by the oxidation of catechols in the presence of ammonia. The product is a dark pink MBTH-quinone compound. This method is very sensitive and positive to catechol even at the 0.05 microgram level and the final product is chemically stable.

这种定位生物样品中儿茶酚残基的技术是基于贝索恩的腙(3-甲基-2-苯并噻唑啉酮肼盐酸盐(MBTH))与儿茶酚在氨存在下氧化得到的醌残基的缩合。该产品是一种深粉红色的四氢甲酚醌化合物。该方法对儿茶酚在0.05微克水平下也具有很高的灵敏度和阳性反应,且最终产物化学性质稳定。
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引用次数: 1
A rapid method for removing silver from stained chromosomes. 从染色染色体上快速去除银的方法。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108043
T E Denton
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引用次数: 3
Sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells. 植物细胞核DNA序列测定及核仁银染色。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108037
J Dolezel, J Cíhalíková, O V Zakchlenjuk

A method for sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells is described. Feulgen staining is done first and nuclear DNA estimated by absorption cytophotometry. Following this, the slides are stained with AgNO3. The method has been used to study the process of nucleolar fusion in garlic (Allium sativum L.) meristem root tip cells. It was found that during interphase nucleoli rarely fused, thus most fusion must have occurred before the G1 phase of the cell cycle.

介绍了一种植物细胞核DNA序列测定和核仁银染色的方法。首先进行Feulgen染色,然后通过吸收细胞光度法估计核DNA。随后,用AgNO3染色载玻片。用该方法对大蒜分生组织根尖细胞的核仁融合过程进行了研究。在间期核仁很少融合,因此大多数融合发生在细胞周期的G1期之前。
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引用次数: 2
Fixative evaluation and histologic appearance of embryonic rodent tissue. 啮齿动物胚胎组织的固定物评价和组织学外观。
Pub Date : 1989-01-01 DOI: 10.3109/10520298909108036
W B Howard, C C Willhite, R A Smart

Histopathologic study of early hamster embryos was carried out after fixation in Zenker's solution, alcoholic formalin, Bouin's fluid, 10% neutral buffered formalin, or 3% glutaraldehyde and staining with hematoxylin and eosin. Fixation in Zenker's fluid followed by postfixation in neutral buffered formalin provided superior preservation of normal embryonic subcellular detail as compared to the other candidate processing techniques.

采用Zenker溶液、含酒精福尔马林、Bouin液、10%中性缓冲福尔马林或3%戊二醛固定,苏木精和伊红染色,对早期仓鼠胚胎进行组织病理学研究。与其他候选处理技术相比,在Zenker氏液中固定,然后在中性缓冲福尔马林中固定,可以更好地保存正常胚胎亚细胞的细节。
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引用次数: 3
期刊
Stain technology
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