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The Disgorgement Remedy of Design Patent Law 外观设计专利法的侵权救济
Pub Date : 2019-02-01 DOI: 10.2139/ssrn.3353536
Pamela Samuelson, Mark P. Gergen
U.S. design patent law has a unique statutory remedy that allows patentees to recover an infringer’s “total profit” on sales of “articles of manufacture” to which infringing designs have been applied. In the high-profile Samsung v. Apple case, the U.S. Supreme Court decided that the relevant article of manufacture could be either a component of an end product (i.e., the flat face of an electronic device) or the end product (i.e., smartphones). However, it provided no guidance about how to decide what the relevant article is or how to measure profits from sales of them. Upon remand, a jury awarded Apple $533 million for Samsung’s infringement of three patents on small external design features of smartphones. This was 25% higher than the “total profit” award that the Supreme Court vacated when rendering its Samsung decision. We are not alone in regarding this award as excessive and indeed punitive. This Article scrutinizes the legislative history of the total profit disgorgement remedy and offers guidance about how the disgorgement remedy in design patent cases should be analyzed and applied going forward. “Total profit” disgorgement on end products may have been an appropriate remedy when patented designs covered the overall appearance of end products (e.g., carpet or wallpaper designs), as we believe that they did when this special remedy was enacted. The explicit premise was that the infringer contributed (almost) nothing of value to the end product. The nature of the design entitlement has, however, changed over the past 40 years in two key respects. First and most important, the entitlement has fragmented so that ever smaller design elements of end products are being patented. Second, patents are issuing for some designs that are more functional than ornamental. These changes have enabled design patentees to claim total profit disgorgement even when the entire profit on sales of end products is manifestly not attributable to the infringed designs, as was the case in Samsung. The Article explains why and how courts should handle issues of causation and apportionment in applying the disgorgement remedy. Generally, total profits on an end product should be awarded only when none or very little of the profit is causally attributable to the defendant’s contributions to the infringing enterprise. When the defendant’s profit is attributable to the combination of its contributions to the infringing enterprise and the use of the infringing design, the initial problem is identifying profits the defendant would have made without use of the infringing design. The plaintiff is not entitled to any profits attributable to the defendant’s contributions. A second problem is to identify profits arising from the combination of the defendant’s contributions and its use of the infringing design. These profits should be apportioned. The Article concludes with recommendations about reforms. It argues that judges should decide the relevant article of manufac
美国外观设计专利法有一项独特的法定补救措施,允许专利权人从侵权设计所应用的“制成品”的销售中收回侵权人的“全部利润”。在备受瞩目的三星诉苹果案中,美国最高法院裁定,相关制造品可以是最终产品(即电子设备的平面)的组件,也可以是最终产品(即智能手机)的组件。然而,它没有就如何确定相关商品是什么或如何衡量相关商品的销售利润提供指导。在发回法庭后,陪审团裁定三星侵犯了苹果在智能手机外部设计方面的三项专利,并判给苹果5.33亿美元。这比最高法院在对三星做出判决时撤销的“总利润”裁决高出25%。我们并不是唯一认为这一裁决过度而且实际上是惩罚性的。本文对利润总额分割救济的立法历史进行了梳理,为今后如何分析和运用外观设计专利案件中的分割救济提供了指导。当专利设计涵盖最终产品的整体外观(例如,地毯或墙纸设计)时,对最终产品的“总利润”分类可能是一种适当的补救措施,正如我们认为,当这种特殊补救措施颁布时,它们确实是这样做的。明确的前提是,侵权人对最终产品(几乎)没有贡献任何价值。然而,在过去40年里,设计权利的性质在两个关键方面发生了变化。首先,也是最重要的一点是,专利权利已经支离破碎,最终产品的设计元素越来越小。其次,一些功能性大于装饰性的设计正在获得专利。这些变化使得外观设计专利权人能够要求获得全部利润,即使最终产品销售的全部利润显然不属于侵权设计,就像三星的情况一样。本文阐述了法院在适用分割救济时,为什么以及如何处理因果关系和分配问题。一般来说,只有当最终产品的全部利润没有或很少的利润可归因于被告对侵权企业的贡献时,才应判给被告。当被告的利润可归因于其对侵权企业的贡献和使用侵权设计时,首先要解决的问题是确定被告在不使用侵权设计的情况下所能获得的利润。原告无权从被告的贡献中获得任何利润。第二个问题是确定被告的贡献和使用侵权设计所产生的利润。这些利润应该分配。文章最后提出了改革建议。认为法官应将相关的制造物问题作为审前权利要求解释的一部分予以裁定。由于在知识产权案件中,追缴利润历来是一种衡平法上的补救措施,因此法官也应决定应追缴的利润。但是,如果试图向陪审团提出撤销,则应指示他们在外观设计专利案件的裁决中适用撤销原则。
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引用次数: 0
Transferrin-Guided Polymersomal Doxorubicin for Potent and Low-Toxic Chemotherapy of Orthotopic Hepatocellular Carcinoma in Vivo 转铁蛋白引导聚合体阿霉素在原位肝细胞癌体内强效低毒化疗中的应用
Pub Date : 2019-01-30 DOI: 10.2139/ssrn.3325398
Yaohua Wei, Xiaolei Gu, Liang Cheng, F. Meng, G. Storm, Z. Zhong
Hepatocellular carcinoma (HCC) remains one of the most lethal malignancies. The current chemotherapy with typically low tumor uptake and high toxicity reveals a poor anti-HCC efficacy. Here, we report transferrin-guided polycarbonate based polymersomal doxorubicin (Tf-Ps-Dox) as a low-toxic and potent nanotherapeutics for effective treatment of transferrin receptor (TfR)-positive human liver tumor SMMC-7721 models. Tf-Ps-Dox was facilely fabricated with small sizes of ca. 75 nm and varying Tf densities from 2.2% to 7.0%, by post-modification of maleimide-functionalized Ps-Dox (Dox loading content of 10.6 wt.%) with thiolated transferrin. MTT assays showed that Tf-Ps-Dox had an optimal Tf surface density of 3.9%. The cellular uptake, intracellular Dox level, and anticancer efficacy of Tf-Ps-Dox to SMMC-7721 cells were inhibited by supplementing free transferrin, supporting that Tf-Ps-Dox is endocytosed via TfR. Interestingly, Tf-Ps-Dox exhibited a high accumulation of 8.5% ID/g in subcutaneous SMMC-7721 tumors, which was 2 and 3-fold higher than non-targeted Ps-Dox and clinically used liposomal Dox formulation (Lipo-Dox), respectively. The median survival times of mice bearing orthotopic SMMC-7721 tumors increased from 82, 88 to 96 days when treated with Tf-Ps-Dox at Dox doses from 8, 12 to 16 mg/kg, which was significantly better than Ps-Dox at 8 mg/kg (58 days) and Lipo-Dox at 4 mg/kg (48 days) or PBS (36 days). Notably, unlike Lipo-Dox, no body weight loss and damage to major organs could be discerned for all Tf-Ps-Dox groups, indicating that Tf-Ps-Dox causes low systemic toxicity. This transferrin-dressed polymersomal doxorubicin provides a potent and low-toxic treatment modality for human hepatocellular carcinoma.
肝细胞癌(HCC)是最致命的恶性肿瘤之一。目前的化疗具有典型的低肿瘤摄取和高毒性,表明抗hcc的效果较差。在这里,我们报道了转铁蛋白引导的聚碳酸酯基聚合体多柔比星(Tf-Ps-Dox)作为一种低毒有效的纳米疗法,可有效治疗转铁蛋白受体(TfR)阳性的人肝脏肿瘤SMMC-7721模型。通过用硫代转铁蛋白修饰马来酰亚胺功能化的Ps-Dox (Dox负载量为10.6 wt.%),制备出尺寸约为75 nm的Tf-Ps-Dox, Tf密度从2.2%到7.0%不等。MTT试验表明,Tf- ps - dox的最佳Tf表面密度为3.9%。通过补充游离转铁蛋白,可抑制Tf-Ps-Dox对SMMC-7721细胞的细胞摄取、细胞内Dox水平和抗癌功效,支持Tf-Ps-Dox通过TfR被内吞。有趣的是,Tf-Ps-Dox在皮下SMMC-7721肿瘤中表现出8.5% ID/g的高蓄积,分别比非靶向Ps-Dox和临床使用的脂质体Dox制剂(lipoo -Dox)高2倍和3倍。当接受8、12至16 mg/kg剂量的Tf-Ps-Dox治疗时,原位SMMC-7721肿瘤小鼠的中位生存时间从82、88至96天增加,显著优于8 mg/kg剂量的Ps-Dox(58天)、4 mg/kg剂量的lipop -Dox(48天)或PBS(36天)。值得注意的是,与lipoo - dox不同,所有Tf-Ps-Dox组均未发现体重减轻和主要器官损伤,这表明Tf-Ps-Dox引起的全身毒性较低。这种转铁蛋白修饰的聚合体阿霉素为人类肝细胞癌提供了一种有效的低毒治疗方式。
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引用次数: 0
Research on Pig Transportation Management System Model Based on RFID and Sensor Technology 基于RFID和传感器技术的生猪运输管理系统模型研究
Pub Date : 2019-01-18 DOI: 10.2139/ssrn.3319659
Hao Liu, Zhong Yao, Wenwen Zhou, Fu Tao Zhao
As one of the most commonly meat products, the quality of pork is directly related to people's health. Pig transportation is the key link between breeding farms and slaughterhouses. However, starvation and plague often occur in reality, which not only brings economic losses to farms and slaughterhouses but also brings some food safety risks. This paper analyzed the problems in the transportation process of pigs and puts forward a pig transportation management system model based on RFID and sensor technology under the background of the rapid development of information technology. The system model was analyzed and elaborated in detail from four key business models of slaughter, loading, transportation and receiving. At the same time, the article evaluates the system model from three aspects: problems resolution, economic benefit, and social benefit, which provide some references for the practical application of the system model.
猪肉作为最常见的肉制品之一,其质量直接关系到人们的身体健康。生猪运输是养殖场和屠宰场之间的关键环节。然而,现实中经常发生饥饿和瘟疫,这不仅给养殖场和屠宰场带来经济损失,也带来了一定的食品安全风险。本文分析了生猪运输过程中存在的问题,在信息技术快速发展的背景下,提出了一种基于RFID和传感器技术的生猪运输管理系统模型。从屠宰、装载、运输和接收四个关键业务模型对系统模型进行了详细的分析和阐述。同时,从问题解决、经济效益和社会效益三个方面对系统模型进行评价,为系统模型的实际应用提供参考。
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引用次数: 1
Structural Transformation and Embrittlement During Lithiation and Delithiation Cycles in an Amorphous Silicon Electrode 非晶硅电极锂化和去硫循环过程中的结构转变和脆化
Pub Date : 2019-01-11 DOI: 10.2139/ssrn.3314057
Swastik Basu, N. Koratkar, Yunfeng Shi
Abstract Silicon shows potential as an anode material in lithium ion batteries due to its high specific capacity, yet its considerable volume expansion during lithiation leads to fracture and pulverization. Unfortunately, neither the atomic-level structural evolution, nor the mechanical behavior of the anode during lithiation and delithiation cycles is well understood. Interestingly, the lithiation process of a-Si provides an interesting continuum from open-structured network glass to densely-packed atomic glass, which could be used to obtain useful insights regarding commonalities in glasses. Here atomic level simulation has been used to investigate one cycle of lithiation and delithiation of amorphous silicon electrode, using Grand Canonical Monte Carlo (GCMC) and molecular dynamics (MD) simulations. The atomic level structural transformation and damage accumulation of the anode during cycling has been systematically analyzed, as well as their mechanical responses in compact tension tests. There appears to be a ductile-brittle-ductile transition for the amorphous silicon anode during both the lithiation and delithiation cycle. In other words, amorphous silicon is particularly vulnerable at intermediate lithiation. The fracture behavior of lithiated silicon was found to correlate to the Poisson's ratio, due to variations in bond covalency and structural disorder.
摘要硅具有很高的比容量,具有作为锂离子电池负极材料的潜力,但其在锂化过程中的体积膨胀会导致断裂和粉化。不幸的是,无论是原子水平的结构演变,还是阳极在锂化和衰竭循环中的力学行为都没有得到很好的理解。有趣的是,a-Si的锂化过程提供了一个有趣的连续体,从开放结构的网络玻璃到密集堆积的原子玻璃,这可以用来获得关于玻璃共性的有用见解。本文利用大正则蒙特卡罗(GCMC)和分子动力学(MD)模拟,采用原子水平模拟研究了非晶硅电极的锂化和去锂化的一个循环过程。系统地分析了循环过程中阳极的原子级结构转变和损伤积累,以及它们在紧致拉伸试验中的力学响应。非晶硅阳极在锂化和耗光循环过程中都表现出延性-脆性-延性的转变。换句话说,非晶硅在中间锂化时特别脆弱。由于化学键共价的变化和结构的无序性,硅的断裂行为与泊松比有关。
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引用次数: 19
Importance of Technology Acceptance Assessment for Successful Implementation and Development of New Technologies 技术验收评估对新技术成功实施和开发的重要性
Pub Date : 2019-01-08 DOI: 10.33552/GJES.2019.01.000511
Hamed Taherdoost
This editorial article is studying the importance of technology acceptance assessment for successful implementation and development of New Technologies. Theory of Reasoned Action (TRA), Theory of Interpersonal Behaviour (TIB), Social Cognitive Theory (SCT), Theory of Planned Behavior (TPB), Diffusion of Innovation Theory (DIT), Technology Acceptance Model (TAM), Extension of TAM, Motivational Model (MM), Uses and Gratification Theory (UGT), Model of PC Utilization (MPCU), Igbaria’s Model, Unified Theory of Acceptance and Use of Technology (UTAUT), Compatibility UTAUT (C-UTAUT), Perceived Characteristics of Innovating Theory (PCIT) and E-Service Technology Acceptance Model (ESAM).
这篇社论研究了技术验收评估对新技术成功实施和开发的重要性。理性行为理论(TRA)、人际行为理论(TIB)、社会认知理论(SCT)、计划行为理论(TPB)、创新扩散理论(DIT)、技术接受模型(TAM)、技术接受模型(TAM)的扩展、动机模型(MM)、使用与满足理论(UGT)、个人电脑利用模型(MPCU)、Igbaria模型、技术接受与使用统一理论(UTAUT)、兼容性理论(C-UTAUT)、创新理论感知特征与电子服务技术接受模型
{"title":"Importance of Technology Acceptance Assessment for Successful Implementation and Development of New Technologies","authors":"Hamed Taherdoost","doi":"10.33552/GJES.2019.01.000511","DOIUrl":"https://doi.org/10.33552/GJES.2019.01.000511","url":null,"abstract":"This editorial article is studying the importance of technology acceptance assessment for successful implementation and development of New Technologies. Theory of Reasoned Action (TRA), Theory of Interpersonal Behaviour (TIB), Social Cognitive Theory (SCT), Theory of Planned Behavior (TPB), Diffusion of Innovation Theory (DIT), Technology Acceptance Model (TAM), Extension of TAM, Motivational Model (MM), Uses and Gratification Theory (UGT), Model of PC Utilization (MPCU), Igbaria’s Model, Unified Theory of Acceptance and Use of Technology (UTAUT), Compatibility UTAUT (C-UTAUT), Perceived Characteristics of Innovating Theory (PCIT) and E-Service Technology Acceptance Model (ESAM).","PeriodicalId":220342,"journal":{"name":"Materials Science Educator: Courses","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115099005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Equilibrium of Intellectual Property Rights Under Fair Use: Case Study of Copyright Law and Trade Secrets Law Derivation of Reverse Engineering in Developing Countries 合理使用下的知识产权均衡:以发展中国家逆向工程的著作权法和商业秘密法衍生为例
Pub Date : 2019-01-01 DOI: 10.2139/ssrn.3546322
Walaiwan Mathurotpreechakun
The balancing of interest is an important objective according to Article 7 of the TRIPS Agreement, which requires the States Parties to implement the provisions of international law in the national legal system. However, each country faces differences in social, economic, political, and cultural backgrounds, especially differences of the developed countries when compared to the developing countries. There is a significant difference in the use of intellectual property law in each country. Therefore, the criteria for the exception of reverse engineering vary from country to country. The purpose of this research is to study the equilibrium of intellectual property rights under fair use derivation from reverse engineering in a developing economy. This study leads to the proposed recommendations for the criterion of intellectual property rights protection for reverse engineering in developing countries and the legislative amendment into two Thai Acts which in the Copyright Act B.E. 2537 (1994), and in the Trade Secret Act B.E. 2545 (2002). Thus, the development of economy in developing countries should amend the intellectual property law that related to reverse engineering.
根据《与贸易有关的知识产权协定》第7条,利益平衡是一个重要的目标,它要求缔约国在国家法律体系中实施国际法的规定。然而,每个国家都面临着社会、经济、政治和文化背景的差异,特别是发达国家与发展中国家的差异。在知识产权法的运用上,每个国家都有很大的不同。因此,逆向工程的例外标准因国而异。本研究的目的是研究发展中经济体中逆向工程合理使用下的知识产权均衡。本研究为发展中国家逆向工程的知识产权保护标准提出了建议,并对泰国《版权法》B.E. 2537(1994)和《商业秘密法》B.E. 2545(2002)两项法案进行了立法修订。因此,发展中国家的经济发展需要修改与逆向工程相关的知识产权法。
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引用次数: 0
Investigation of Tribological Behaviour of Journal Bearings for Different Conditions : A Review 不同工况下滑动轴承摩擦学性能研究综述
Pub Date : 2018-12-13 DOI: 10.2139/ssrn.3323680
Mangesh Saraf, B. Kothavale
In high speed applications like pumps, turbines, turbocharger journal bearing serviceability is very important one. The journal bearing acting as a support for high rotary speeds as well as acting as a damping element so as to absorb the vibrations at high speeds. In hydrodynamic region if failure of lubrication or starved lubrication is occurred it responsible for tremendous amount of friction between the shaft and bearing with high wear loss causing damage to the surfaces of the component. So it focuses that use of sintered composites for journal bearing applications is suitable for reduced frictional wear. However experimental analysis is required for sintered composites at high speed with starved or dry conditions of lubrication. The combine effect of parameters has to be studied for tribological behaviour of sintered journal bearings. It includes surface quality, alloying material compositions, porosity of sintered bearings, geometrical aspects and external conditions like applied loads, speed transitions, temperature, lubricating conditions etc. With reference to said subject thorough literature review was carried out. It provides very valuable information regarding parametric analysis and its effect on friction and wear of bearings in different situations of temperature, load, speeds etc.
在泵、涡轮等高速应用中,涡轮增压器轴颈轴承的可使用性是非常重要的一项。轴颈轴承作为高转速的支撑,也作为阻尼元件,以吸收高速时的振动。在流体动力区,如果发生润滑失效或缺油,则轴与轴承之间会产生巨大的摩擦,磨损大,导致部件表面损坏。因此,重点是使用烧结复合材料用于滑动轴承应用,以减少摩擦磨损。然而,需要对高速烧结复合材料在饥渴或干燥润滑条件下进行实验分析。烧结滑动轴承的摩擦学性能需要研究参数的综合影响。它包括表面质量,合金材料成分,烧结轴承的孔隙率,几何方面和外部条件,如施加载荷,速度转变,温度,润滑条件等。针对上述主题,我们进行了全面的文献综述。它提供了关于参数分析及其在不同温度、负载、速度等情况下对轴承摩擦和磨损的影响的非常有价值的信息。
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引用次数: 1
Stability Analysis of Various Lengths Conical Hydrodynamic Bearing for Variable Load Conditions 变载荷条件下不同长度锥形动压轴承稳定性分析
Pub Date : 2018-12-13 DOI: 10.2139/ssrn.3331447
A. Gangrade, V. Phalle, S. S. Mantha
Conical hydrodynamic journal bearing is used to sustain radial load and observed to adjust the effect of axial load on rotating elements. Small compact size conical hydrodynamic fluid film bearings are used in many applications, such as compact hard disc drive. Hence need arises to explore the performance of these bearing for variable load condition and various sizes in terms of aspect ratio. These conical bearings also emerge with better feature to replace two separate bearings (journal and thrust bearings) in various applications. The present study proposed the stability analysis of conical hydrodynamic journal bearing in terms of stiffness and damping coefficients for bearing of semi cone angle (γ = 30o) and bearing size in terms of aspect ratio (λ = 0.5, 0.8, 1.0) for a wide range of radial load ((W_r ) ̅=0.1-0.9) on rotating journal at a speed of 500 rpm. Finite element method has been used to solve the modified Reynolds equation in spherical coordinate system to investigate the flow of lubricant in the clearance space. Stability parameters for various configuration of conical hydrodynamic bearing have been presented and discussed in terms of direct and cross coupled stiffness coefficients, damping coefficients and threshold speed margin. Results show that threshold speed (ω ̅_th) of conical journal bearing is improved for short bearings when aspect ratio changes from 1.0 to 0.5.
锥形滑动轴承用于承受径向载荷,并观察调节轴向载荷对旋转元件的影响。小紧凑尺寸锥形流体动力流体膜轴承用于许多应用,如紧凑的硬盘驱动器。因此,有必要研究这些轴承在变载荷条件下和不同尺寸的长径比下的性能。这些圆锥轴承也出现了更好的功能,以取代两个独立的轴承(轴颈和推力轴承)在各种应用。在转速为500转/分的大范围径向载荷((W_r) =0.1-0.9)下,采用半锥角(γ = 30o)轴承的刚度和阻尼系数,以及长径比(λ = 0.5, 0.8, 1.0)的轴承尺寸,对锥形滑动轴承进行了稳定性分析。采用有限元法在球坐标系下求解修正雷诺方程,研究润滑油在间隙空间中的流动。从直接耦合和交叉耦合刚度系数、阻尼系数和阈值速度裕度等方面,给出了锥形动液轴承不同构型的稳定性参数,并进行了讨论。结果表明:当长径比在1.0 ~ 0.5范围内变化时,短轴圆锥滑动轴承的阈值转速(ω _th)有所提高;
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引用次数: 1
Effect of Marble Dust as Filler on Erosion Behaviour of Needle-punched-nonwoven Jute/Epoxy Composite 大理石粉尘填料对针刺非织造黄麻/环氧复合材料侵蚀性能的影响
Pub Date : 2018-12-13 DOI: 10.2139/ssrn.3322501
Ankush P. Sharma, Aditya Purohit, R. Nagar, A. Patnaik
In current work, solid-particle erosion wear of the needle-punched-nonwoven jute felt epoxy (NPNWJ/E) composite filled with waste marble powder (MP) particulates is investigated. The composites are fabricated in controlled condition using vacuum-assisted-resin-transfer-molding (VARTM) technique with 0, 10 20, and 30wt. % of waste marble powder. The influence of test parameters: velocity (30-60 m/s), impingement angle (30°- 90°) and erodent size (75-180 μm) are studied using air jet erosion test rig. The experiments are performed using orthogonal array L16 (45) and prominent control factors affecting the erosion rate were determined using ANOVA. The study reveals that the increasing content of marble dust in composite reduces the erosion wear rate. The maximum erosion rate is recorded at 60° impingement angle and shows the semi ductile behavior. Surface analysis of the eroded samples is performed through a scanning electron microscope (SEM) to understand the erosion mechanism meticulously of the particulate filled composites. The eroded surfaces resulting from air-jet erosion were analyzed using scanning electron microscope (SEM) for greater understanding of erosion mechanism meticulously of the particulate filled composites.
本文研究了废大理石粉(MP)颗粒填充的针刺-无纺布环氧黄麻毡(NPNWJ/E)复合材料的固粒冲蚀磨损。复合材料是在控制条件下使用真空辅助树脂转移成型(VARTM)技术制造的,重量分别为0、10、20和30。%的废大理石粉。在空气射流冲蚀试验台上,研究了速度(30 ~ 60 m/s)、冲蚀角(30°~ 90°)和冲蚀尺寸(75 ~ 180 μm)对冲蚀性能的影响。实验采用正交阵列L16(45)进行,并采用方差分析确定影响侵蚀率的主要控制因素。研究表明,复合材料中大理岩粉含量的增加降低了冲蚀磨损率。在60°冲击角处记录到最大冲蚀速率,并表现出半延性。通过扫描电子显微镜(SEM)对侵蚀样品进行表面分析,详细了解颗粒填充复合材料的侵蚀机理。利用扫描电子显微镜(SEM)对喷气冲蚀引起的冲蚀表面进行了分析,以更好地了解颗粒填充复合材料的冲蚀机理。
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引用次数: 10
In Vitro Conservation and Comparative GC-MS Analysis of Roots of Mother and Micropropagated Plant of Withania Somnifera Withania Somnifera母株与小苗根的离体保存及GC-MS对比分析
Pub Date : 2018-12-12 DOI: 10.2139/ssrn.3299847
N. Sharma, Felicia Lalremruati, Vanlalmalsawmi Vanlalmalsawmi, N. Kaur
Withania somnifera is an important medicinal plant utilized for several medicinal purposes. Due to poor germination rate, over exploitation and habitat destruction the plant has become critically endangered. In the present study intermodal segments were utilized for development of simple and efficient protocol for mass propagation of the plant. Development of callus was achieved onto 2,4-D supplemented medium. Regeneration of shoots from callus was achieved onto MS BAP 2,4-D. In vitro regenerated shoots were successfully rooted onto ½ MS IAA. about 78.4&% plants survived during transplantation. Methanolic extract of roots of mother and in vitro regenerated plant were subjected to GC-MS analysis which revealed comparatively higher number of phytocompounds in roots of mother plant.
Withania somnifera是一种具有多种药用价值的重要药用植物。由于发芽率低,过度开发和生境破坏,该植物已处于极度濒危状态。在本研究中,利用多式联运片段来开发简单有效的植物大量繁殖方案。愈伤组织在2,4- d培养基上发育。在MS bap2,4 - d培养基上实现了愈伤组织的再生。离体再生芽成功地生根在½MS IAA上。移植成活率约为78.4 %。用GC-MS对母株和离体再生植株的甲醇提取物进行分析,发现母株根系中含有较多的植物化合物。
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引用次数: 0
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