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Biodegradation of Seaweed-based Bioplastics Using Deep-sea Marine Bacterial Consortia 利用深海海洋细菌群生物降解海藻基生物塑料
Pub Date : 2024-06-18 DOI: 10.1007/s41208-024-00736-9
M. P. Sudhakar, M. Hinduja, K. Sujitha, A. Ganesh Kumar, G. Dharani

Thin plastic films used for packing food materials are unsafe for consumers and are not readily degradable. Single-use plastic films accumulate in the environment and cause adverse effects in the food chain. In this study, Kappaphycus alvarezii, which has the value-added polymer carrageenan, was used for developing a bioplastic film along with the plasticizer polyethylene glycol (PEG 3000). Different concentrations of seaweed were used (3%, 4% and 5% dry weight), of which 4% had a higher tensile strength than the other concentrations. The physical and mechanical properties of the developed plastic films, such as thickness, tensile strength (TS), water vapor transmission rate (WVTR), oxygen transmission rate (OTR) and color, were tested for packaging applications in the food industry. A higher concentration of seaweed increased the WVTR, and a lower concentration increased the OTR. In addition, the biodegradation of the developed bioplastic was tested using isolated deep-sea microbial consortia to meet environmental standards. A deep-sea marine microbial consortium (Bacillus paralicheniformis G1, Bacillus subtilis G2, Bacillus subtilis Z1, and Enterobacter cloacae subsp. dissolvens Z2) degrades seaweed (Kappaphycus alvarezii)-derived bioplastic under buried soil conditions. The maximum degradation (88%) in the 5% (w/v) bioplastic film was observed within 10 days of incubation.

Graphical Abstract

用于包装食品材料的薄塑料薄膜对消费者不安全,而且不易降解。一次性塑料薄膜会在环境中累积,并对食物链造成不良影响。在这项研究中,使用了具有高附加值聚合物卡拉胶的 Kappaphycus alvarezii 和增塑剂聚乙二醇(PEG 3000)来开发生物塑料薄膜。使用了不同浓度的海藻(干重分别为 3%、4% 和 5%),其中 4% 的海藻比其他浓度的海藻具有更高的拉伸强度。测试了所开发塑料薄膜的物理和机械性能,如厚度、拉伸强度(TS)、水蒸气透过率(WVTR)、氧气透过率(OTR)和颜色,以用于食品行业的包装应用。海藻浓度越高,水蒸气透过率越高,而浓度越低,氧气透过率越高。此外,还利用分离的深海微生物群对所开发生物塑料的生物降解进行了测试,以达到环保标准。深海海洋微生物菌群(枯草芽孢杆菌 G1、枯草芽孢杆菌 G2、枯草芽孢杆菌 Z1 和溶解性肠杆菌 Z2)在埋土条件下降解海藻(Kappaphycus alvarezii)衍生的生物塑料。在 5%(w/v)生物塑料薄膜中,培养 10 天内降解率最高(88%)。
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引用次数: 0
Health Risk Assessment and Mineral Contents in Brown Seaweed Colpomenia sinuosa for Human Consumption 供人类食用的褐藻中的健康风险评估和矿物质含量
Pub Date : 2024-06-01 DOI: 10.1007/s41208-024-00724-z
Gülen Türker, İlknur Ak, Elif Çağrı Taş

This study focuses on the mineral content and health risk assessment of the brown seaweed Colpomenia sinuosa. The mineral composition of the seaweed samples (Na, K, Mg, Fe, Cu, Zn, Mn, P, Ni, Pb, Cd, Cr, Al) was investigated from three different Turkish coastal locations: İzmir Bay (S1), the Strait of Çanakkale (S2), and the Sea of Marmara (S3). The mineral contents of samples were analyzed using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) as part of our methodology. It was found that the mineral content varied significantly depending on the location from which the samples were taken. The highest levels of Na, Ca, Mg, Fe, Ni, and Mn were observed in the samples from İzmir Bay, whereas those from the Sea of Marmara showed elevated levels of P and Zn. The study also revealed the presence of heavy metals, including Pb, Co, Cd, Cr, and Al, in the seaweed. According to hazard index (HI) calculations, adult consumption of this seaweed poses a low health risk, with a hazard index below 1. Various heavy metals have been detected in the seaweed, but their concentrations are not high enough to pose significant health risks, and therefore, the seaweed is safe for human consumption within reasonable limits. Our results suggest that C. sinuosa could be a valuable source of essential minerals for human consumption.

本研究的重点是褐海藻(Colpomenia sinuosa)的矿物质含量和健康风险评估。研究调查了土耳其三个不同沿海地区海藻样本的矿物质成分(Na、K、Mg、Fe、Cu、Zn、Mn、P、Ni、Pb、Cd、Cr、Al):伊兹密尔湾(S1)、恰纳卡莱海峡(S2)和马尔马拉海(S3)。作为研究方法的一部分,我们使用电感耦合等离子体原子发射光谱(ICP-AES)分析了样本中的矿物质含量。结果发现,矿物质含量因采样地点的不同而有很大差异。伊兹密尔湾的样本中 Na、Ca、Mg、Fe、Ni 和 Mn 含量最高,而马尔马拉海的样本中 P 和 Zn 含量较高。研究还发现,海藻中存在重金属,包括铅、钴、镉、铬和铝。根据危害指数(HI)计算,成人食用这种海藻的健康风险较低,危害指数低于 1。虽然在海藻中检测到了多种重金属,但其浓度不足以对健康造成重大危害,因此在合理范围内,人类食用这种海藻是安全的。我们的研究结果表明,C. sinuosa 可以成为人类食用的重要必需矿物质来源。
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引用次数: 0
First record of the rare deep-sea shark, Scymnodon ichiharai Yano and Tanaka, 1984 (Squaliformes: Somniosidae) from Andaman Waters, India 印度安达曼水域罕见深海鲨鱼 Scymnodon ichiharai Yano and Tanaka, 1984 (Squaliformes: Somniosidae) 的首次记录
Pub Date : 2024-05-30 DOI: 10.1007/s41208-024-00723-0
Ravi Ranjan Kumar, S. Venu, K. K. Bineesh, K. A. Sajeela, D. A. Ebert, A. Gopalakrishnan, V. S. Basheer

The Japanese velvet dogfish Scymnodon ichiharai Yano and Tanaka 1984 (Somniosidae) is a rare deep-sea shark hitherto known only from the northwest Pacific Ocean. This species is observed for the first time from the northern Indian Ocean. Two male specimens with total length (TL) of 760 mm, and 323 mm were caught by longline hook in the commercial motorized longline boat fishery at a depth of 400 m off Port Blair, Andaman Islands, India. We provide morphological descriptions of S. ichiharai from India in comparison with formerly identified specimens along with DNA barcodes from this region. The capture of this species in the northern Indian Ocean is a considerable biogeographic range extension and confirms the first record of S. ichiharai from the Indian EEZ.

日本天鹅绒狗鱼(Scymnodon ichiharai Yano and Tanaka 1984)(Somniosidae)是一种罕见的深海鲨鱼,迄今为止只在西北太平洋发现过。这是首次在北印度洋观察到该物种。我们在印度安达曼群岛布莱尔港附近 400 米水深的商业机动延绳钓船上用延绳钓钩捕获了两条雄性标本,其总长度(TL)分别为 760 毫米和 323 毫米。我们提供了来自印度的 S. ichiharai 的形态学描述,并与以前鉴定的标本以及该地区的 DNA 条形码进行了比较。在印度洋北部捕获该物种是生物地理范围的一次相当大的扩展,并证实了印度专属经济区的首次 S. ichiharai 记录。
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引用次数: 0
Stock Assessment of Exploited Populations of Shovel-Nosed Lobster Thenus unimaculatus (Scyllaridae, Burton and Davie, 2007) Along the Indian Coast Using Length-Based Approaches 使用基于长度的方法评估印度沿岸锹鼻龙虾 Thenus unimaculatus(Scyllaridae,Burton 和 Davie,2007 年)的已开发种群数量
Pub Date : 2024-05-30 DOI: 10.1007/s41208-024-00727-w
Rekha Devi Chakraborty, Rajan Kumar, Gyanaranjan Dash, Gidda Maheswarudu

This manuscript presents a detailed investigation into the stock status of Thenus unimaculatus, a commercially valuable lobster species in India. In data-poor fisheries, Bayesian approach offers robust guidance for sustainable sand lobster population management. Utilizing a non-conventional Bayesian approach, the study employed length-based Bayesian biomass (LBB) and length-based Spawning Potential Ratio (LB-SPR) methods to analyze length frequency data collected between 2011 and 2019. In our study using LBB, the current exploited biomass (B/B0 = 0.35) was found to be lower than the biomass capable of producing maximum sustainable yield (B/BMSY=0.93). Spawning potential ratio was calculated between 28 and 37% using LB-SPR model. The fishing mortality (F/M = 1.5) was slightly above optimal, resulting in low sand lobster biomass due to moderate over-exploitation. This suggests a need for rational slipper lobster catch control. However, with an Lm50/Lc50 ratio close to 1.0, indicates an absence of targeted juvenile fishery, it suggest the need to reduce fishing pressure. . However, implementing measures is challenging because the species forms a by-catch in trawl fisheries.

本手稿详细调查了印度具有商业价值的龙虾物种 Thenus unimaculatus 的种群状况。在数据匮乏的渔业中,贝叶斯方法为沙龙虾种群的可持续管理提供了有力指导。本研究采用非常规贝叶斯方法,使用基于长度的贝叶斯生物量(LBB)和基于长度的产卵潜能比(LB-SPR)方法来分析2011年至2019年期间收集的长度频率数据。在我们使用贝叶斯生物量法进行的研究中,发现当前已开发生物量(B/B0=0.35)低于能够产生最大可持续产量的生物量(B/BMSY=0.93)。利用 LB-SPR 模型计算的产卵潜能率介于 28% 和 37% 之间。捕捞死亡率(F/M = 1.5)略高于最佳值,导致中度过度开发造成沙龙虾生物量较低。这表明需要合理控制滑龙虾的捕捞量。然而,由于 Lm50/Lc50 比率接近 1.0,表明缺乏有针对性的幼鱼捕捞,因此需要减少捕捞压力。.然而,由于该物种是拖网渔业的副渔获物,因此实施措施具有挑战性。
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引用次数: 0
Cadmium Effects on Superoxide Dismutase Activity in Litopenaeus vannamei Hemolymph 镉对凡纳滨对虾血淋巴中超氧化物歧化酶活性的影响
Pub Date : 2024-05-29 DOI: 10.1007/s41208-024-00726-x
Juan Carlos Bautista-Covarrubias, Germán Velarde-Montes, Martín Soto-Jiménez, Marisela Aguilar-Juárez, Cristina Osuna-Martínez, Isidro Osuna-López, José Armando López-Sánchez, Martín Gabriel Frías-Espericueta

Under controlled laboratory conditions the Cd effects on hemolymph superoxide dismutase (SOD) activity were evaluated at 8.8, 24.9 and 249 µg Cd/L and exposure times of 5, 48, and 96 h. Shrimp hemolymph samples were extracted from the first abdominal segment and hemocytes separated by centrifugation, and SOD activity (units/mg of protein in shrimp hemolymph) was quantified using the RANSOD assay kit. No mortality was observed in the controls and treatments. Superoxide dismutase activity ranged from 0.938 ± 0.0938 to 0.946 ± 0.042 SOD units/mg of protein in shrimp hemolymph in controls. At 5-h exposure, SOD activity decreased at all Cd concentrations, with significant differences (p < 0.05) than those of controls. However, a significant (p < 0.05) SOD activity increase was observed at 48-h exposure in those shrimp exposed to 24.9 and 249 µg Cd/L; but a significant (p < 0.05) SOD decrease was determined in those specimens exposed to highest Cd levels at 96-h exposure. More experiments such as those in this study are required for future updates of protection criteria for Cd in estuarine and marine environments.

在受控实验室条件下,评估了镉对血淋巴超氧化物歧化酶(SOD)活性的影响,镉含量分别为 8.8、24.9 和 249 微克/升,接触时间分别为 5、48 和 96 小时。从对虾第一腹节提取血淋巴样本,离心分离血细胞,使用 RANSOD 检测试剂盒量化 SOD 活性(对虾血淋巴中的单位/毫克蛋白质)。对照组和处理组均未观察到死亡现象。对照组对虾血淋巴中的超氧化物歧化酶活性为 0.938 ± 0.0938 至 0.946 ± 0.042 SOD 单位/毫克蛋白质。接触 5 小时后,所有镉浓度下的 SOD 活性均下降,与对照组相比差异显著(p < 0.05)。然而,在暴露于 24.9 和 249 µg Cd/L 的对虾中,暴露 48 小时后 SOD 活性明显增加(p < 0.05);但在暴露于最高镉浓度的对虾中,暴露 96 小时后 SOD 活性明显降低(p < 0.05)。今后更新河口和海洋环境中镉的保护标准时,需要进行更多类似本研究的实验。
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引用次数: 0
Pomacanthidae Species in the Mediterranean and a New Location Record of the Emperor Angelfish Pomacanthus imperator (Bloch, 1787) from these Species 地中海中的帝王 Angelfish Pomacanthus imperator (Bloch, 1787) 物种以及这些物种的新位置记录
Pub Date : 2024-05-27 DOI: 10.1007/s41208-024-00728-9
Alper Yıldız, Mehmet Gökoğlu

Angelfish (Pomacanthidae) are fish distributed in reef areas in the tropical and semi-tropical regions of the Atlantic and Indo-Pacific oceans. Up to now, five different species of this particular species have been identified in the Mediterranean, but there are suspicions that one of them is misidentified. The Emperor angelfish (Pomacanthus imperator) is the most frequently reported species from the Pomacanthidae family in the Mediterranean region. This study presents a new record (36°44’34.59"N, 31°24’6.42"E) of the Emperor Angelfish from the Antalya Gulf in the Mediterranean. The recent sighting of P. imperator in the Gulf of Antalya marks its eighth location in the Mediterranean, its second on the Turkish coast, and its first in the Gulf of Antalya. Following this research, the count of fish species originating from the Red Sea found in Antalya Bay has now reached 71. When the location records of this fish in the Mediterranean are examined, it is seen that it has expanded its distribution towards the west.

天使鱼(Pomacanthidae)是一种分布在大西洋和印度洋热带和半热带地区珊瑚礁区的鱼类。迄今为止,在地中海地区已发现该物种的五个不同品种,但有人怀疑其中一个品种被误认。皇帝天使鱼(Pomacanthus imperator)是地中海地区最常报道的天使鱼科物种。本研究提供了一条来自地中海安塔利亚湾的皇帝天使鱼新记录(北纬 36°44'34.59",东经 31°24'6.42")。最近在安塔利亚湾发现帝王 Angelfator 标志着它在地中海的第八次出现,第二次出现在土耳其海岸,第一次出现在安塔利亚湾。在这项研究之后,在安塔利亚湾发现的来自红海的鱼类物种已达 71 种。对这种鱼类在地中海的分布记录进行研究后发现,它的分布范围已经向西扩展。
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引用次数: 0
First Record of Partial Albinism in the Chinese Fanray, Platyrhina Sinensis (Elasmobranchii: Myliobatiformes: Platyrhinidae) 中国扇鳐首次发现部分白化现象(鳞鳃目:贻贝形目:鲽科)
Pub Date : 2024-05-18 DOI: 10.1007/s41208-024-00713-2
Yan Zhuang, Shuting Ke, Mingru Chen, Jiamei Xiao

A female albino fanray specimen was collected by trawl from the southern Taiwan Strait on April 11, 2022. This unique individual was identified as the Chinese fanray, Platyrhina sinensis (Bloch & Schneider, 1801), based on morphological identification and DNA barcoding. It was classified as partial albinism (leucism) due to the absence of pigmentation in the skin and normal pigmentation in the irises. This specimen represents the first reported case of albinism within the family Platyrhinidae, providing valuable insights for physiological and ecological research on albino fish.

2022 年 4 月 11 日,通过拖网从台湾海峡南部采集到一只雌性白化扇鳐标本。根据形态学鉴定和 DNA 条形码,这只独特的个体被鉴定为中国扇鳐,Platyrhina sinensis (Bloch & Schneider, 1801)。由于皮肤没有色素,而虹膜色素正常,因此被归类为部分白化病(白化病)。该标本是 Platyrhinidae 科内首次报道的白化病例,为白化鱼的生理和生态研究提供了宝贵的见解。
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引用次数: 0
Body and Otolith Morphometrics of Sea Toad Chaunax Abei (Le Danois, 1978) in Suruga Bay, Japan 日本骏河湾海蟾 Chaunax Abei(Le Danois,1978 年)的身体和耳石形态计量学
Pub Date : 2024-05-16 DOI: 10.1007/s41208-024-00715-0
A. M. Şenbahar, Akira Eto, Masashi Yokota
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引用次数: 0
Seasonal Variability On Cross-shore Profile in Meso-tidal Settings Due to Lunar Cycle Effects in Kuala Terengganu Coast 瓜拉丁加奴海岸月相周期效应导致的中潮位跨岸剖面的季节性变化
Pub Date : 2024-05-13 DOI: 10.1007/s41208-024-00705-2
Muhammad Fadhli Harris, Muhammad Izuan Nadzri, Ku Mohd Kalkausar Ku Yusof, Wan Amrul Jaahiz Abd Razak, Mohamad Hakim Mohd Shukri, Nor Bakhiah Baharim, Azizi Ali, Effi Helmy Ariffin

Kuala Terengganu, one of the coastal zones in Malaysia, is prone to natural hazard factors, such as coastal erosion which may affect the morphological condition of the beaches in Terengganu. The beach morphological changes were driven by the seasonal monsoon such as Northeast Monsoon (NEM) and Southwest Monsoon (SWM). Hence, to understand the short-temporal morphological response of meso-tidal beaches during spring tide events throughout the lunar cycle phase, the study was investigated in Batu Buruk Beach, Kuala Terengganu. The coastal profiles, particularly during spring tide (Full Moon and New Moon phases) were collected from five (5) transects starting on October 2020 to October 2021. The monitoring of cross-shore was conducted using Real time Kinematic (RTK) to measure the cross-sectional area and sediment volume changes. The data collected were computed using profiler 3.2XL software. MIKE-21 numerical modeling software was used to simulate tidal elevation within the period of this study. The results showed that Batu Buruk Beach received significant impact from the lunar cycle, especially on sediment gain and loss. The cross-sectional analysis revealed positive sediment gains during the Full Moon. However, during the New Moon phase, erosion seemed to take place around the upper swash zone area. This provides an insight into the spring tide erosion and accretion and contributes some understanding to beach recovery processes.

瓜拉丁加奴是马来西亚沿海地区之一,很容易受到自然灾害因素的影响,如海岸侵蚀可能会影响丁加奴海滩的形态状况。海滩形态的变化受季节性季风(如东北季风(NEM)和西南季风(SWM))的影响。因此,为了了解中潮汐海滩在整个农历周期春潮期间的短时形态反应,研究人员在瓜拉丁加奴的峇都布路克海滩进行了调查。从 2020 年 10 月到 2021 年 10 月,从五(5)个横断面收集了海岸剖面图,特别是春潮期间(满月和新月阶段)的剖面图。跨海岸监测使用实时运动学(RTK)测量横截面积和沉积物体积变化。收集到的数据使用剖面仪 3.2XL 软件进行计算。MIKE-21 数值建模软件用于模拟本研究期间的潮汐高程。研究结果表明,峇都布路克海滩受到月圆周期的显著影响,尤其是沉积物的增减。横截面分析表明,在满月期间,沉积物会增加。然而,在新月阶段,上斜坡区周围似乎出现了侵蚀。这提供了对春潮侵蚀和吸积的深入了解,有助于对海滩恢复过程的理解。
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引用次数: 0
On the Identity of a rare Morphologically Abnormal Butterfly ray from the east Coast of India Resolved Through Molecular tool 通过分子工具确定印度东海岸一种罕见的形态异常蝴蝶射线的身份
Pub Date : 2024-05-08 DOI: 10.1007/s41208-024-00717-y
Rajesh Kumar Behera, Swarup Ranjan Mohanty, Smrutirekha Acharya, Sandeep Kumar Mohapatra, Subhrendu Sekhar Mishra, K. K Bineesh, Anil Mohapatra

A free-swimming longtail butterfly ray, Gymnura poecilura (Shaw, 1804), specimen with significant abnormality was observed from the Bay of Bengal along the Odisha coast, east coast of India. One specimen, collected from Arjyapalli fish landing Centre along the Sothern Odisha coast, has the pectoral fins on both sides evenly split or symmetrically bifurcated for which initially the identity of the specimen was confusing. When classical taxonomy could not resolve the confusion, molecular analysis helped in obtaining species identity, which was confirmed as Gymnura poecilura. Pectoral fin splitting abnormality in the Gymnuridae family is reported herewith for the first time.

在印度东海岸奥迪沙沿岸的孟加拉湾观察到一条自由游动的长尾蝶魟标本(Gymnura poecilura (Shaw, 1804)),该标本有明显的异常。其中一个标本是从奥迪沙南部海岸的 Arjyapalli 鱼类上岸中心采集的,其胸鳍两侧均匀分裂或对称分叉,起初该标本的身份令人困惑。当传统分类学无法解决这一困惑时,分子分析帮助确定了物种身份,并确认为 Gymnura poecilura。本文首次报道了鲷科鱼类的胸鳍分裂异常。
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引用次数: 0
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Thalassas: An International Journal of Marine Sciences
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