Md. Mehadi Hasan Sohag, Olivier Nicoud, Racha Amine, Abir Khalil-Mgharbel, J. Alcaraz, I. Vilgrain, Donald K. Martin
Abstract The goal of this study was to determine whether the Tethapod system, which was designed to determine the impedance properties of lipid bilayers, could be used for cell culture in order to utilise micro-impedance spectroscopy to examine further biological applications. To that purpose we have used normal epithelial cells from kidney (RPTEC) and a kidney cancer cell model (786-O). We demonstrate that the Tethapod system is compatible with the culture of 10,000 cells seeded to grow on a small area gold measurement electrode for several days without affecting the cell viability. Furthermore, the range of frequencies for EIS measurements were tuned to examine easily the characteristics of the cell monolayer. We demonstrate significant differences in the paracellular resistance pathway between normal and cancer kidney epithelial cells. Thus, we conclude that this device has advantages for the study of cultured cells that include (i) the configuration of measurement and reference electrodes across a microfluidic channel, and (ii) the small surface area of 6 parallel measurement electrodes (2.1 mm2) integrated in a microfluidic system. These characteristics might improve micro-impedance spectroscopy measurement techniques to provide a simple tool for further studies in the field of the patho-physiology of biological barriers.
{"title":"Improved micro-impedance spectroscopy to determine cell barrier properties","authors":"Md. Mehadi Hasan Sohag, Olivier Nicoud, Racha Amine, Abir Khalil-Mgharbel, J. Alcaraz, I. Vilgrain, Donald K. Martin","doi":"10.2478/ebtj-2020-0017","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0017","url":null,"abstract":"Abstract The goal of this study was to determine whether the Tethapod system, which was designed to determine the impedance properties of lipid bilayers, could be used for cell culture in order to utilise micro-impedance spectroscopy to examine further biological applications. To that purpose we have used normal epithelial cells from kidney (RPTEC) and a kidney cancer cell model (786-O). We demonstrate that the Tethapod system is compatible with the culture of 10,000 cells seeded to grow on a small area gold measurement electrode for several days without affecting the cell viability. Furthermore, the range of frequencies for EIS measurements were tuned to examine easily the characteristics of the cell monolayer. We demonstrate significant differences in the paracellular resistance pathway between normal and cancer kidney epithelial cells. Thus, we conclude that this device has advantages for the study of cultured cells that include (i) the configuration of measurement and reference electrodes across a microfluidic channel, and (ii) the small surface area of 6 parallel measurement electrodes (2.1 mm2) integrated in a microfluidic system. These characteristics might improve micro-impedance spectroscopy measurement techniques to provide a simple tool for further studies in the field of the patho-physiology of biological barriers.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47112337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Endophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for drug development. Pseudomonas aeruginosa is one of the major causes of healthcare-associated bacterial infections, leading to increased mortality and morbidity. In this study, isolated endophytic fungi from Cola nitida were screened for anti-pseudomonas properties. Endophytic fungi associated with healthy leaves of C. nitida were isolated using standard methods. Fungi were identified through their morphological, cultural and microscopic characteristics. The fungi were subjected to solid-state fermentation and secondary metabolites extracted using ethyl acetate and concentrated under vacuum. The crude extracts were screened for antimicrobial activity against clinical and laboratory strains of Pseudomonas aeruginosa using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi; Acremonium sp., Aspergillus sp. and Trichophyton sp. were isolated. At 1 mg/ml, extracts of the three fungi displayed antipseudomonal activity with inhibition zone diameter ranging from 6 - 4 mm. HPLC-DAD analysis revealed the presence of compounds, such as 4-hydroxyphenyl acetate. indole-3-acetic acid, and protocatechuic acid among others in the fungal extracts. The findings in this study reveal that endophytic fungi associated with C. nitida possess promising antipseudomonal properties. This finding can open new doors for the discovery of new agents against P. aeruginosa.
{"title":"Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa","authors":"D. Nwobodo, C. Ihekwereme, F. Okoye","doi":"10.2478/ebtj-2020-0019","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0019","url":null,"abstract":"Abstract Endophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for drug development. Pseudomonas aeruginosa is one of the major causes of healthcare-associated bacterial infections, leading to increased mortality and morbidity. In this study, isolated endophytic fungi from Cola nitida were screened for anti-pseudomonas properties. Endophytic fungi associated with healthy leaves of C. nitida were isolated using standard methods. Fungi were identified through their morphological, cultural and microscopic characteristics. The fungi were subjected to solid-state fermentation and secondary metabolites extracted using ethyl acetate and concentrated under vacuum. The crude extracts were screened for antimicrobial activity against clinical and laboratory strains of Pseudomonas aeruginosa using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi; Acremonium sp., Aspergillus sp. and Trichophyton sp. were isolated. At 1 mg/ml, extracts of the three fungi displayed antipseudomonal activity with inhibition zone diameter ranging from 6 - 4 mm. HPLC-DAD analysis revealed the presence of compounds, such as 4-hydroxyphenyl acetate. indole-3-acetic acid, and protocatechuic acid among others in the fungal extracts. The findings in this study reveal that endophytic fungi associated with C. nitida possess promising antipseudomonal properties. This finding can open new doors for the discovery of new agents against P. aeruginosa.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46147248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-28DOI: 10.22541/au.159064938.87006424
S. Temel, M. C. Ergoren, Izel Yilmaz, Ozel Yuruker, H. Çobanogullari, O. Tosun, H. Oral, N. Bahçeci̇ler
Abstract Objective: Allergic asthma is the most frequently observed subtype of individuals with asthma. The effects of serotonin plays in the pathophysiology of asthma has not been clearly determined. Thus, this study aimed to investigate the association between the 5HTR2C gene rs 6318 G>C polymorphism and allergic asthma in pediatric patients in Cyprus. Methods: This study included total number of 177 individuals with 118 control and 59 pediatric patients (43 atopic and 16 non-atopic asthma patient). A skin prick test was performed for each patient to confirm asthma diagnosis and to evaluate atopic status. Genotyping for the 5HTR2C was completed by Real Time-PCR analysis. Results: The genotype distribution frequencies were not in agreement with the Hardy-Weinberg Equilibrium in the patients’ group (p<0.00001). The frequency of the risk allele (allele C) was not significantly different between the patient and control groups (p=0.255). The genotypic distribution between atopic asthma and non-atopic asthma within the patientsts groups was not in agreement with the Hardy-Weinberg Equilibrium (p=0.006). However, risk allele presence showed a statistically significant association with atopy-related asthma (p=0.037). Discussion & Conclusion: Overall, despite the finding of no association between the 5HTR2C rs6318 C allele and childhood asthma, the current results indicated that there is a strong association between the 5HTR2C rs6318 C variant and childhood atopic asthma.
{"title":"Association of the 5HTR2C gene Ser23 variation with childhood allergic asthma","authors":"S. Temel, M. C. Ergoren, Izel Yilmaz, Ozel Yuruker, H. Çobanogullari, O. Tosun, H. Oral, N. Bahçeci̇ler","doi":"10.22541/au.159064938.87006424","DOIUrl":"https://doi.org/10.22541/au.159064938.87006424","url":null,"abstract":"Abstract Objective: Allergic asthma is the most frequently observed subtype of individuals with asthma. The effects of serotonin plays in the pathophysiology of asthma has not been clearly determined. Thus, this study aimed to investigate the association between the 5HTR2C gene rs 6318 G>C polymorphism and allergic asthma in pediatric patients in Cyprus. Methods: This study included total number of 177 individuals with 118 control and 59 pediatric patients (43 atopic and 16 non-atopic asthma patient). A skin prick test was performed for each patient to confirm asthma diagnosis and to evaluate atopic status. Genotyping for the 5HTR2C was completed by Real Time-PCR analysis. Results: The genotype distribution frequencies were not in agreement with the Hardy-Weinberg Equilibrium in the patients’ group (p<0.00001). The frequency of the risk allele (allele C) was not significantly different between the patient and control groups (p=0.255). The genotypic distribution between atopic asthma and non-atopic asthma within the patientsts groups was not in agreement with the Hardy-Weinberg Equilibrium (p=0.006). However, risk allele presence showed a statistically significant association with atopy-related asthma (p=0.037). Discussion & Conclusion: Overall, despite the finding of no association between the 5HTR2C rs6318 C allele and childhood asthma, the current results indicated that there is a strong association between the 5HTR2C rs6318 C variant and childhood atopic asthma.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44377246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Around 70 infectious agents are possible threats for blood safety. The risk for blood recipients is increasing because of new emergent agents like West Nile, Zika and Chikungunya viruses, or parasites such as Plasmodium and Trypanosoma cruzi in non-endemic regions, for instance. Screening programmes of the donors are more and more implemented in several Countries, but these cannot prevent completely infections, especially when they are caused by new agents. Pathogen inactivation (PI) methods might overcome the limits of the screening and different technologies have been set up in the last years. This review aims to describe the most widely used methods focusing on their efficacy as well as on the preservation integrity of blood components.
{"title":"New strategies for the control of infectious and parasitic diseases in blood donors: the impact of pathogen inactivation methods","authors":"L. Galli, F. Bruschi","doi":"10.2478/ebtj-2020-0007","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0007","url":null,"abstract":"Abstract Around 70 infectious agents are possible threats for blood safety. The risk for blood recipients is increasing because of new emergent agents like West Nile, Zika and Chikungunya viruses, or parasites such as Plasmodium and Trypanosoma cruzi in non-endemic regions, for instance. Screening programmes of the donors are more and more implemented in several Countries, but these cannot prevent completely infections, especially when they are caused by new agents. Pathogen inactivation (PI) methods might overcome the limits of the screening and different technologies have been set up in the last years. This review aims to describe the most widely used methods focusing on their efficacy as well as on the preservation integrity of blood components.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42306352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Matei, S. Matei, G. Matei, G. Cogălniceanu, C. Cornea
Abstract Silver nanoparticles (AgNPs) are nanomaterials obtained by nanotechnology and due to their antimicrobial properties have a major importance in the control of various species of bacteria, fungi and viruses, with applications in medicine, cosmetics or food industry. The goal of the paper was to present the results of the research carried out on rapid extracellular biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria Lactobacillus sp. strain LCM5 and to assess the antimicrobial activity. Analysis of transmission electron microscopy (TEM) micrographs evidenced that the size of AgNPs synthesized using culture filtrates of lactic acid bacteria strain LCM5 ranged between 3 and 35 nm diameter, with an average particle size of 13.84±4.56 nm. AgNPs presented a good dispersion, approximately spherical shape, with parallel stripes certifying crystal structure. Frequency distribution revealed that preponderant dimensions of biosynthesized AgNPs were below 20 nm (94%). Antimicrobial activity of AgNPs was variable depending on both species and group of test microorganisms (bacteria or fungi) involved. Diameter of growth inhibition zone of Aspergillus flavus and Aspergillus ochraceus caused by silver nanoparticles synthesized by lactic acid bacteria strain LCM5 were similar (12.39 ± 0.61mm and 12.86 ± 0.78 mm) but significant stronger inhibition was registered against Penicillium expansum (15.87 ± 1.01mm). The effectiveness of biosynthesized silver nanoparticles was more pronounced against Gram-negative bacteria Chromobacterium violaceum with larger zone of inhibition (18 ± 0.69 mm diameter) when compared to those from fungi. Results recommend the silver nanoparticles biosynthesized using culture filtrate of the lactic acid bacteria Lactobacillus sp. strain LCM5 for biotechnological purposes, as promising antimicrobial agents.
{"title":"Biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria, characterization and antifungal activity","authors":"A. Matei, S. Matei, G. Matei, G. Cogălniceanu, C. Cornea","doi":"10.2478/ebtj-2020-0011","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0011","url":null,"abstract":"Abstract Silver nanoparticles (AgNPs) are nanomaterials obtained by nanotechnology and due to their antimicrobial properties have a major importance in the control of various species of bacteria, fungi and viruses, with applications in medicine, cosmetics or food industry. The goal of the paper was to present the results of the research carried out on rapid extracellular biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria Lactobacillus sp. strain LCM5 and to assess the antimicrobial activity. Analysis of transmission electron microscopy (TEM) micrographs evidenced that the size of AgNPs synthesized using culture filtrates of lactic acid bacteria strain LCM5 ranged between 3 and 35 nm diameter, with an average particle size of 13.84±4.56 nm. AgNPs presented a good dispersion, approximately spherical shape, with parallel stripes certifying crystal structure. Frequency distribution revealed that preponderant dimensions of biosynthesized AgNPs were below 20 nm (94%). Antimicrobial activity of AgNPs was variable depending on both species and group of test microorganisms (bacteria or fungi) involved. Diameter of growth inhibition zone of Aspergillus flavus and Aspergillus ochraceus caused by silver nanoparticles synthesized by lactic acid bacteria strain LCM5 were similar (12.39 ± 0.61mm and 12.86 ± 0.78 mm) but significant stronger inhibition was registered against Penicillium expansum (15.87 ± 1.01mm). The effectiveness of biosynthesized silver nanoparticles was more pronounced against Gram-negative bacteria Chromobacterium violaceum with larger zone of inhibition (18 ± 0.69 mm diameter) when compared to those from fungi. Results recommend the silver nanoparticles biosynthesized using culture filtrate of the lactic acid bacteria Lactobacillus sp. strain LCM5 for biotechnological purposes, as promising antimicrobial agents.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45564821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Najjaa, B. Abdelkarim, E. Doria, Abdelbasset Boubakri, N. Trabelsi, H. Falleh, H. Tlili, M. Neffati
Abstract Plants have been seen to possess the potential to be excellent biological matrices to serve as a basis for investigating the presence of promising therapeutic agents for cancer treatment. Several successful anti-cancer medicines - or their analogues - nowadays in use are plant derived and many more are under clinical trials. Under current circumstances, the purpose of this work was to test aqueous and ethanolic extracts of five aromatic and medicinal plants from arid zones on some tumor cell lines. These plants, Cymbopogon schoenanthus (L.) Spreng, Crithmum maritimum (L.) Spreng, Hammada scoparia (Pomel) Iljin, Retama raetam (Forssk.) and Zizyphus lotus (L.) Desf., widely used in Tunisian ethnomedicine, were assessed for their phenolic compounds, antioxidants and anticancer activities in aqueous and ethanol extracts. Total polyphenols, flavonoid and tannin contents were determined colorimetrically and some of these molecules were identified using RP-HPLC. A significant difference on phenolic contents and composition were found among the investigated plants. Cymbopogon schoenanthus was the richest in phenolic compounds (approx. 72%) with quercetine-3-o-rhamnoside (approx. 33%) as main contributor. For all the tested plants, the highest antioxidant capacity was detected in the ethanolic extracts rather than in the aqueous ones. The highest antiproliferative potential was observed for the ethanolic extracts. Hammada scoparia, Retama raetam and Zizyphus lotus exhibited important antiproliferative effect that reached 67% at a 1% extract concentration. Taken together, the present study supports the potential development of chemotherapeutic agents from, at least, four of the five studied Tunisian ethnomedicinal plants.
{"title":"Phenolic composition of some Tunisian medicinal plants associated with anti-proliferative effect on human breast cancer MCF-7 cells","authors":"H. Najjaa, B. Abdelkarim, E. Doria, Abdelbasset Boubakri, N. Trabelsi, H. Falleh, H. Tlili, M. Neffati","doi":"10.2478/ebtj-2020-0012","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0012","url":null,"abstract":"Abstract Plants have been seen to possess the potential to be excellent biological matrices to serve as a basis for investigating the presence of promising therapeutic agents for cancer treatment. Several successful anti-cancer medicines - or their analogues - nowadays in use are plant derived and many more are under clinical trials. Under current circumstances, the purpose of this work was to test aqueous and ethanolic extracts of five aromatic and medicinal plants from arid zones on some tumor cell lines. These plants, Cymbopogon schoenanthus (L.) Spreng, Crithmum maritimum (L.) Spreng, Hammada scoparia (Pomel) Iljin, Retama raetam (Forssk.) and Zizyphus lotus (L.) Desf., widely used in Tunisian ethnomedicine, were assessed for their phenolic compounds, antioxidants and anticancer activities in aqueous and ethanol extracts. Total polyphenols, flavonoid and tannin contents were determined colorimetrically and some of these molecules were identified using RP-HPLC. A significant difference on phenolic contents and composition were found among the investigated plants. Cymbopogon schoenanthus was the richest in phenolic compounds (approx. 72%) with quercetine-3-o-rhamnoside (approx. 33%) as main contributor. For all the tested plants, the highest antioxidant capacity was detected in the ethanolic extracts rather than in the aqueous ones. The highest antiproliferative potential was observed for the ethanolic extracts. Hammada scoparia, Retama raetam and Zizyphus lotus exhibited important antiproliferative effect that reached 67% at a 1% extract concentration. Taken together, the present study supports the potential development of chemotherapeutic agents from, at least, four of the five studied Tunisian ethnomedicinal plants.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43714510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract A new and simple micelles-rich restricted access supramolecular solvent-based liquid phase microextraction method (RASUPRASs-LPME) based on the ion-pair complex formation of phosphate (PO43-) ions with ammonium heptamolybdate and malachite green in acidic medium was developed. The phosphate ion concentration after microextraction of the ion-pair complex to the hexagonal aggregates of decanoic acid (DA) was measured with micro-volume UV-Vis spectrophotometer at 625 nm. All analytical parameters which are effective on the method such as acid type and concentration, supramolecular solvent volume, amount of the components forming the complex, sample volume, were optimized. The preconcentration factor (PF), limit of detection (LOD) and limit of quantification (LOQ) for the developed method was found to be 15, 9.6 and 32.1, respectively. The RA-SUPRAs-LPME method was finally applied for the analysis of the phosphate content of different types of water samples.
基于磷酸(PO43-)离子与七钼酸铵和孔雀石绿在酸性介质中形成离子对络合物，建立了一种新的、简单的、富胶束的限制性进入超分子溶剂型液相微萃取方法(RASUPRASs-LPME)。用微体积紫外-可见分光光度计在625 nm处测定了离子对配合物微萃取到癸酸(DA)六方聚集体后的磷酸离子浓度。对酸的种类和浓度、超分子溶剂的体积、络合物组分的量、样品体积等对该方法有影响的分析参数进行了优化。该方法的预富集因子(PF)、检出限(LOD)和定量限(LOQ)分别为15、9.6和32.1。最后应用ra - supra - lpme法对不同类型水样的磷酸盐含量进行分析。
{"title":"Micelle-based restricted access ion-pair microextraction of phosphate at trace levels in water samples for separation, preconcentration and determination","authors":"M. Soylak, Haldun Hilmi Gorucu, E. Yılmaz","doi":"10.2478/ebtj-2020-0010","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0010","url":null,"abstract":"Abstract A new and simple micelles-rich restricted access supramolecular solvent-based liquid phase microextraction method (RASUPRASs-LPME) based on the ion-pair complex formation of phosphate (PO43-) ions with ammonium heptamolybdate and malachite green in acidic medium was developed. The phosphate ion concentration after microextraction of the ion-pair complex to the hexagonal aggregates of decanoic acid (DA) was measured with micro-volume UV-Vis spectrophotometer at 625 nm. All analytical parameters which are effective on the method such as acid type and concentration, supramolecular solvent volume, amount of the components forming the complex, sample volume, were optimized. The preconcentration factor (PF), limit of detection (LOD) and limit of quantification (LOQ) for the developed method was found to be 15, 9.6 and 32.1, respectively. The RA-SUPRAs-LPME method was finally applied for the analysis of the phosphate content of different types of water samples.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46786033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. Abdelmoneim, Alaa I. Mohammed, Esraa O. Gadim, M. A. Mohammed, Sara H. Hamza, Sara A. Mirghani, T. A. Mahmoud, Mohamed A. Hassan
Abstract Hyperparathyroidism-Jaw Tumor (HPT-JT) is an autosomal dominant disorder with variable expression, with an estimated prevalence of 6.7 per 1,000 population. Genetic testing for predisposing CDC73 (HRPT2) mutations has been an important clinical advance, aimed at early detection and/or treatment to prevent advanced disease. The aim of this study is to assess the most deleterious SNPs mutations on CDC73 gene and to predict their influence on the functional and structural levels using different bioinformatics tools. Method: Computational analysis using twelve different in-silico tools including SIFT, PROVEAN, PolyPhen-2, SNAP2, PhD-SNP, SNPs&GO, P-Mut, I-Mutant ,Project Hope, Chimera, COSMIC and dbSNP Short Genetic Variations were used to identify the impact of mutations in CDC73 gene that might be causing jaw tumor. Results: From (733) SNPs identified in the CDC73 gene we found that only Eleven SNPs (G49C, L63P, L64P, D90H, R222G, W231R, P360S, R441C, R441H, R504S and R504H) has deleterious effect on the function and structure of protein and expected to cause the syndrome. Conclusion: Eleven substantial genetic/molecular aberrations in CDC73 gene identified that could serve as diagnostic markers for hyperparathyroidism-jaw tumor (HPT-JT).
甲状旁腺功能亢进颌骨肿瘤(HPT-JT)是一种常染色体显性遗传病，具有可变的表达，估计患病率为每1000人中6.7人。CDC73 (HRPT2)易感基因检测是一项重要的临床进展，旨在早期发现和/或治疗以预防晚期疾病。本研究的目的是利用不同的生物信息学工具评估CDC73基因上最有害的SNPs突变，并预测其对功能和结构水平的影响。方法:采用SIFT、PROVEAN、polyphen2、SNAP2、PhD-SNP、SNPs&GO、P-Mut、I-Mutant、Project Hope、Chimera、COSMIC和dbSNP Short Genetic Variations等12种不同的计算机工具进行计算分析，确定CDC73基因突变对颌骨肿瘤的影响。结果:从CDC73基因中鉴定的733个snp中，我们发现只有11个snp (G49C、L63P、L64P、D90H、R222G、W231R、P360S、R441C、R441H、R504S和R504H)对蛋白质的功能和结构有有害影响，可能导致该综合征。结论:鉴定出11个CDC73基因显著的遗传/分子畸变，可作为HPT-JT的诊断标记。
{"title":"In silico analysis of CDC73 gene revealing 11 novel SNPs with possible association to Hyperparathyroidism-Jaw Tumor syndrome","authors":"A. H. Abdelmoneim, Alaa I. Mohammed, Esraa O. Gadim, M. A. Mohammed, Sara H. Hamza, Sara A. Mirghani, T. A. Mahmoud, Mohamed A. Hassan","doi":"10.2478/ebtj-2020-0008","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0008","url":null,"abstract":"Abstract Hyperparathyroidism-Jaw Tumor (HPT-JT) is an autosomal dominant disorder with variable expression, with an estimated prevalence of 6.7 per 1,000 population. Genetic testing for predisposing CDC73 (HRPT2) mutations has been an important clinical advance, aimed at early detection and/or treatment to prevent advanced disease. The aim of this study is to assess the most deleterious SNPs mutations on CDC73 gene and to predict their influence on the functional and structural levels using different bioinformatics tools. Method: Computational analysis using twelve different in-silico tools including SIFT, PROVEAN, PolyPhen-2, SNAP2, PhD-SNP, SNPs&GO, P-Mut, I-Mutant ,Project Hope, Chimera, COSMIC and dbSNP Short Genetic Variations were used to identify the impact of mutations in CDC73 gene that might be causing jaw tumor. Results: From (733) SNPs identified in the CDC73 gene we found that only Eleven SNPs (G49C, L63P, L64P, D90H, R222G, W231R, P360S, R441C, R441H, R504S and R504H) has deleterious effect on the function and structure of protein and expected to cause the syndrome. Conclusion: Eleven substantial genetic/molecular aberrations in CDC73 gene identified that could serve as diagnostic markers for hyperparathyroidism-jaw tumor (HPT-JT).","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48149661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The objective of this work is numerical simulation of the membrane by direct analysis at micro, meso and macro level. This approach includes first a defining and modeling of a basic structural unit, after that simulation of a fragment as a representative element of the membrane structure. Then the results obtained to transfer for the entire membrane module and finally modeling of the membrane as porous media with calculated permeability. The numerical simulation was done with Ansys CFX, using the Darcy’s equation for flow through porous media with configuration of the membrane and second order backward Euler transient scheme for solving the Navier-Stokes equations. The permeability of the membrane is determined at a micro and macro level by computer simulation for different fluids, which allows to evaluating the influence of the viscosity on the flow passing through the membrane. This micro-macro approach is quite efficient and cost-effective because it saves time and requires less computer capacity and allows direct analysis of the complex structure of the membrane modules.
{"title":"Micro-macro transition for numerical simulation of submerged membrane bioreactor","authors":"Moutafchìeva Dessislava, Iliev Veselin","doi":"10.2478/ebtj-2020-0009","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0009","url":null,"abstract":"Abstract The objective of this work is numerical simulation of the membrane by direct analysis at micro, meso and macro level. This approach includes first a defining and modeling of a basic structural unit, after that simulation of a fragment as a representative element of the membrane structure. Then the results obtained to transfer for the entire membrane module and finally modeling of the membrane as porous media with calculated permeability. The numerical simulation was done with Ansys CFX, using the Darcy’s equation for flow through porous media with configuration of the membrane and second order backward Euler transient scheme for solving the Navier-Stokes equations. The permeability of the membrane is determined at a micro and macro level by computer simulation for different fluids, which allows to evaluating the influence of the viscosity on the flow passing through the membrane. This micro-macro approach is quite efficient and cost-effective because it saves time and requires less computer capacity and allows direct analysis of the complex structure of the membrane modules.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44802896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract In forests, edaphic microbial communities are involved in litter decomposition and soil forming processes, with major contribution to humification, especially bacteria and fungi being responsible for the main ecosystem services fulfilled by the soil. Research has been carried out aiming to characterize the structure and diversity of microbial communities in the Rendzic Leptosols (WRB) under natural deciduous forest from Visterna, Babadag Plateau and to assess their contribution to ecosystem services provided by soil. The paper presents the results of quantitative estimations and taxonomic composition of soil and litter communities of heterotrophic bacteria and fungi, identification of cellulolytic species, as well as the microbial biomass and global physiological activities expressed as soil respiration potential. More than a half of bacterial species were common in litter and soil (SI=57.14%) and were represented by dominant species of fluorescent or non-fluorescent pseudomonads and Bacillus subtilis but no similarity was found between the two fungal communities. Fungal populations included cosmopolitan species, such as antagonists and strong cellulolytic representatives of genera Penicillium, Trichoderma, Mortierella, Chaetomium, Epicoccum, Aspergillus. Microbial density and microbial biomass presented the highest values in the litter (684 mg C x kg-1 d.s.) and in surface horizon Am1 of soil profile than in the bottom layers. The highest diversity was found in Am1 horizon (0-5 cm) H’=1.983 bits and ε=0.869 for cellulolytic community. Soil respiration reflected the intense physiological activity of microbiome, with high values associated to numerous effectives of bacteria and fungi especially in surface horizon. Microorganisms identified contribute to formation of soil by recycling of nutrients, cellulose decomposition, the synthesis of stable organic matter (humic acids), aggregation of soil particles, biological control of pathogens by antagonistic activity. They improve plant uptake of water and nutrients by forming symbioses (ectomycorrhizae), thus modelling the structure of vegetation.
在森林中，土壤微生物群落参与凋落物分解和土壤形成过程，对腐殖化起主要作用，特别是细菌和真菌承担了土壤的主要生态系统服务功能。本文研究了巴巴达格高原Visterna天然落叶林下Rendzic Leptosols (WRB)微生物群落的结构和多样性，并评价了它们对土壤生态系统服务的贡献。本文介绍了异养细菌和真菌的土壤和凋落物群落的定量估计和分类组成，纤维素水解物种的鉴定，以及微生物生物量和以土壤呼吸势表示的全球生理活动的结果。在凋落物和土壤中常见的细菌种类超过一半(SI=57.14%)，以荧光或非荧光假单胞菌和枯草芽孢杆菌为优势种，但两者之间没有相似性。真菌种群包括世界性的物种，如拮抗菌和强纤维素分解代表的青霉属、木霉属、摩氏菌属、毛菌属、表表皮菌属、曲霉属。微生物密度和微生物生物量在凋落物层(684 mg C x kg-1 d.s.)和土壤剖面的表层Am1均高于底层。群落多样性以Am1层位(0 ~ 5 cm)最高，H′=1.983 bit， ε=0.869。土壤呼吸反映了微生物组强烈的生理活性，其值与细菌和真菌的多种有效作用有关，特别是在表层。已确定的微生物通过营养物质的再循环、纤维素的分解、稳定有机物(腐植酸)的合成、土壤颗粒的聚集、通过拮抗活性对病原体的生物控制来促进土壤的形成。它们通过形成共生(外生菌根)来改善植物对水和养分的吸收，从而模拟植被的结构。
{"title":"Assessing the role of soil microbial communities of natural forest ecosystem","authors":"G. Matei, S. Matei, V. Mocanu","doi":"10.2478/ebtj-2020-0001","DOIUrl":"https://doi.org/10.2478/ebtj-2020-0001","url":null,"abstract":"Abstract In forests, edaphic microbial communities are involved in litter decomposition and soil forming processes, with major contribution to humification, especially bacteria and fungi being responsible for the main ecosystem services fulfilled by the soil. Research has been carried out aiming to characterize the structure and diversity of microbial communities in the Rendzic Leptosols (WRB) under natural deciduous forest from Visterna, Babadag Plateau and to assess their contribution to ecosystem services provided by soil. The paper presents the results of quantitative estimations and taxonomic composition of soil and litter communities of heterotrophic bacteria and fungi, identification of cellulolytic species, as well as the microbial biomass and global physiological activities expressed as soil respiration potential. More than a half of bacterial species were common in litter and soil (SI=57.14%) and were represented by dominant species of fluorescent or non-fluorescent pseudomonads and Bacillus subtilis but no similarity was found between the two fungal communities. Fungal populations included cosmopolitan species, such as antagonists and strong cellulolytic representatives of genera Penicillium, Trichoderma, Mortierella, Chaetomium, Epicoccum, Aspergillus. Microbial density and microbial biomass presented the highest values in the litter (684 mg C x kg-1 d.s.) and in surface horizon Am1 of soil profile than in the bottom layers. The highest diversity was found in Am1 horizon (0-5 cm) H’=1.983 bits and ε=0.869 for cellulolytic community. Soil respiration reflected the intense physiological activity of microbiome, with high values associated to numerous effectives of bacteria and fungi especially in surface horizon. Microorganisms identified contribute to formation of soil by recycling of nutrients, cellulose decomposition, the synthesis of stable organic matter (humic acids), aggregation of soil particles, biological control of pathogens by antagonistic activity. They improve plant uptake of water and nutrients by forming symbioses (ectomycorrhizae), thus modelling the structure of vegetation.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43590232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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