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Micelle-based restricted access ion-pair microextraction of phosphate at trace levels in water samples for separation, preconcentration and determination 基于胶束的限制进入离子对微萃取,用于分离、预富集和测定水样中痕量磷酸盐
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-04-01 DOI: 10.2478/ebtj-2020-0010
M. Soylak, Haldun Hilmi Gorucu, E. Yılmaz
Abstract A new and simple micelles-rich restricted access supramolecular solvent-based liquid phase microextraction method (RASUPRASs-LPME) based on the ion-pair complex formation of phosphate (PO43-) ions with ammonium heptamolybdate and malachite green in acidic medium was developed. The phosphate ion concentration after microextraction of the ion-pair complex to the hexagonal aggregates of decanoic acid (DA) was measured with micro-volume UV-Vis spectrophotometer at 625 nm. All analytical parameters which are effective on the method such as acid type and concentration, supramolecular solvent volume, amount of the components forming the complex, sample volume, were optimized. The preconcentration factor (PF), limit of detection (LOD) and limit of quantification (LOQ) for the developed method was found to be 15, 9.6 and 32.1, respectively. The RA-SUPRAs-LPME method was finally applied for the analysis of the phosphate content of different types of water samples.
基于磷酸(PO43-)离子与七钼酸铵和孔雀石绿在酸性介质中形成离子对络合物,建立了一种新的、简单的、富胶束的限制性进入超分子溶剂型液相微萃取方法(RASUPRASs-LPME)。用微体积紫外-可见分光光度计在625 nm处测定了离子对配合物微萃取到癸酸(DA)六方聚集体后的磷酸离子浓度。对酸的种类和浓度、超分子溶剂的体积、络合物组分的量、样品体积等对该方法有影响的分析参数进行了优化。该方法的预富集因子(PF)、检出限(LOD)和定量限(LOQ)分别为15、9.6和32.1。最后应用ra - supra - lpme法对不同类型水样的磷酸盐含量进行分析。
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引用次数: 11
In silico analysis of CDC73 gene revealing 11 novel SNPs with possible association to Hyperparathyroidism-Jaw Tumor syndrome CDC73基因的计算机分析揭示了11个可能与甲状旁腺功能亢进性颌骨肿瘤综合征相关的新SNP
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-04-01 DOI: 10.2478/ebtj-2020-0008
A. H. Abdelmoneim, Alaa I. Mohammed, Esraa O. Gadim, M. A. Mohammed, Sara H. Hamza, Sara A. Mirghani, T. A. Mahmoud, Mohamed A. Hassan
Abstract Hyperparathyroidism-Jaw Tumor (HPT-JT) is an autosomal dominant disorder with variable expression, with an estimated prevalence of 6.7 per 1,000 population. Genetic testing for predisposing CDC73 (HRPT2) mutations has been an important clinical advance, aimed at early detection and/or treatment to prevent advanced disease. The aim of this study is to assess the most deleterious SNPs mutations on CDC73 gene and to predict their influence on the functional and structural levels using different bioinformatics tools. Method: Computational analysis using twelve different in-silico tools including SIFT, PROVEAN, PolyPhen-2, SNAP2, PhD-SNP, SNPs&GO, P-Mut, I-Mutant ,Project Hope, Chimera, COSMIC and dbSNP Short Genetic Variations were used to identify the impact of mutations in CDC73 gene that might be causing jaw tumor. Results: From (733) SNPs identified in the CDC73 gene we found that only Eleven SNPs (G49C, L63P, L64P, D90H, R222G, W231R, P360S, R441C, R441H, R504S and R504H) has deleterious effect on the function and structure of protein and expected to cause the syndrome. Conclusion: Eleven substantial genetic/molecular aberrations in CDC73 gene identified that could serve as diagnostic markers for hyperparathyroidism-jaw tumor (HPT-JT).
甲状旁腺功能亢进颌骨肿瘤(HPT-JT)是一种常染色体显性遗传病,具有可变的表达,估计患病率为每1000人中6.7人。CDC73 (HRPT2)易感基因检测是一项重要的临床进展,旨在早期发现和/或治疗以预防晚期疾病。本研究的目的是利用不同的生物信息学工具评估CDC73基因上最有害的SNPs突变,并预测其对功能和结构水平的影响。方法:采用SIFT、PROVEAN、polyphen2、SNAP2、PhD-SNP、SNPs&GO、P-Mut、I-Mutant、Project Hope、Chimera、COSMIC和dbSNP Short Genetic Variations等12种不同的计算机工具进行计算分析,确定CDC73基因突变对颌骨肿瘤的影响。结果:从CDC73基因中鉴定的733个snp中,我们发现只有11个snp (G49C、L63P、L64P、D90H、R222G、W231R、P360S、R441C、R441H、R504S和R504H)对蛋白质的功能和结构有有害影响,可能导致该综合征。结论:鉴定出11个CDC73基因显著的遗传/分子畸变,可作为HPT-JT的诊断标记。
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引用次数: 0
Micro-macro transition for numerical simulation of submerged membrane bioreactor 浸没式膜生物反应器数值模拟的微观-宏观转换
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-04-01 DOI: 10.2478/ebtj-2020-0009
Moutafchìeva Dessislava, Iliev Veselin
Abstract The objective of this work is numerical simulation of the membrane by direct analysis at micro, meso and macro level. This approach includes first a defining and modeling of a basic structural unit, after that simulation of a fragment as a representative element of the membrane structure. Then the results obtained to transfer for the entire membrane module and finally modeling of the membrane as porous media with calculated permeability. The numerical simulation was done with Ansys CFX, using the Darcy’s equation for flow through porous media with configuration of the membrane and second order backward Euler transient scheme for solving the Navier-Stokes equations. The permeability of the membrane is determined at a micro and macro level by computer simulation for different fluids, which allows to evaluating the influence of the viscosity on the flow passing through the membrane. This micro-macro approach is quite efficient and cost-effective because it saves time and requires less computer capacity and allows direct analysis of the complex structure of the membrane modules.
摘要本工作的目的是通过微观、细观和宏观层面的直接分析对膜进行数值模拟。该方法首先包括基本结构单元的定义和建模,然后将碎片模拟为膜结构的代表性元素。然后将所得结果传递给整个膜组件,最后将膜建模为具有计算渗透率的多孔介质。使用Ansys CFX进行了数值模拟,使用具有膜结构的多孔介质中流动的Darcy方程和求解Navier-Stokes方程的二阶反向Euler瞬态格式。通过对不同流体的计算机模拟,在微观和宏观层面上确定了膜的渗透率,从而可以评估粘度对通过膜的流动的影响。这种微观-宏观方法非常有效且具有成本效益,因为它节省了时间并且需要较少的计算机容量,并且允许直接分析膜组件的复杂结构。
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引用次数: 1
Assessing the role of soil microbial communities of natural forest ecosystem 天然森林生态系统土壤微生物群落的作用评价
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0001
G. Matei, S. Matei, V. Mocanu
Abstract In forests, edaphic microbial communities are involved in litter decomposition and soil forming processes, with major contribution to humification, especially bacteria and fungi being responsible for the main ecosystem services fulfilled by the soil. Research has been carried out aiming to characterize the structure and diversity of microbial communities in the Rendzic Leptosols (WRB) under natural deciduous forest from Visterna, Babadag Plateau and to assess their contribution to ecosystem services provided by soil. The paper presents the results of quantitative estimations and taxonomic composition of soil and litter communities of heterotrophic bacteria and fungi, identification of cellulolytic species, as well as the microbial biomass and global physiological activities expressed as soil respiration potential. More than a half of bacterial species were common in litter and soil (SI=57.14%) and were represented by dominant species of fluorescent or non-fluorescent pseudomonads and Bacillus subtilis but no similarity was found between the two fungal communities. Fungal populations included cosmopolitan species, such as antagonists and strong cellulolytic representatives of genera Penicillium, Trichoderma, Mortierella, Chaetomium, Epicoccum, Aspergillus. Microbial density and microbial biomass presented the highest values in the litter (684 mg C x kg-1 d.s.) and in surface horizon Am1 of soil profile than in the bottom layers. The highest diversity was found in Am1 horizon (0-5 cm) H’=1.983 bits and ε=0.869 for cellulolytic community. Soil respiration reflected the intense physiological activity of microbiome, with high values associated to numerous effectives of bacteria and fungi especially in surface horizon. Microorganisms identified contribute to formation of soil by recycling of nutrients, cellulose decomposition, the synthesis of stable organic matter (humic acids), aggregation of soil particles, biological control of pathogens by antagonistic activity. They improve plant uptake of water and nutrients by forming symbioses (ectomycorrhizae), thus modelling the structure of vegetation.
在森林中,土壤微生物群落参与凋落物分解和土壤形成过程,对腐殖化起主要作用,特别是细菌和真菌承担了土壤的主要生态系统服务功能。本文研究了巴巴达格高原Visterna天然落叶林下Rendzic Leptosols (WRB)微生物群落的结构和多样性,并评价了它们对土壤生态系统服务的贡献。本文介绍了异养细菌和真菌的土壤和凋落物群落的定量估计和分类组成,纤维素水解物种的鉴定,以及微生物生物量和以土壤呼吸势表示的全球生理活动的结果。在凋落物和土壤中常见的细菌种类超过一半(SI=57.14%),以荧光或非荧光假单胞菌和枯草芽孢杆菌为优势种,但两者之间没有相似性。真菌种群包括世界性的物种,如拮抗菌和强纤维素分解代表的青霉属、木霉属、摩氏菌属、毛菌属、表表皮菌属、曲霉属。微生物密度和微生物生物量在凋落物层(684 mg C x kg-1 d.s.)和土壤剖面的表层Am1均高于底层。群落多样性以Am1层位(0 ~ 5 cm)最高,H′=1.983 bit, ε=0.869。土壤呼吸反映了微生物组强烈的生理活性,其值与细菌和真菌的多种有效作用有关,特别是在表层。已确定的微生物通过营养物质的再循环、纤维素的分解、稳定有机物(腐植酸)的合成、土壤颗粒的聚集、通过拮抗活性对病原体的生物控制来促进土壤的形成。它们通过形成共生(外生菌根)来改善植物对水和养分的吸收,从而模拟植被的结构。
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引用次数: 9
Compared salt tolerance of five local wheat (Triticum aestivum L.) cultivars of Albania based on morphology, pigment synthesis and glutathione content 基于形态、色素合成和谷胱甘肽含量对阿尔巴尼亚5个地方小麦品种的耐盐性进行比较
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0006
A. Bacu, V. Ibro, Magdalena Nushi
Abstract Stressful saline concentrations in soils affect photosynthesis by damaging pigments, photosystems, components of electron transport system, and enzymes involved in the process. Plants respond through very complex stress adaptation mechanisms including proteome modulation, alterations in pigment content, cell osmotic adjustment and control of ion and water homeostasis mechanisms, which stabilize cytosolic glutathione redox potential, etc. The level of plant sensitivity depends on salt toxicity levels, growth stage, physiological and genetic factors. With aim the investigation of the salinity tolerant cultivars, and for the elucidation of mechanisms underlying this complex biological process, here we analyze the impact of four NaCl concentrations (0-50-100-200mM) in growth parameters (root, shoot and leaves length), pigment content (chla, chlb, carotenoids), and GSH content, during seedling of five bread wheat (Triticum aestivum L.) cultivars in modified Hoagland solutions. Based on biometric parameters, pigment synthesis and GSH content cultivar Nogal is salt-sensitive (growth and pigments reduced); cultivar Viktoria is medium-tolerant (growth partially impaired, pigments constant), cultivar Toborzo and cultivar Suba are medium-tolerant (growth partially impaired, pigments increased), cultivar Dajti salt-tolerant (growth partially impaired/ leaves developed, pigments increased). Quantity of GSH in response to different levels of salinity is cultivar specific, and time of exposure to salinity is in negative correlation to GSH content for all investigated cultivars.
土壤盐胁迫通过破坏色素、光系统、电子传递系统组分和参与光合作用的酶来影响光合作用。植物对胁迫的适应机制非常复杂,包括蛋白质组调节、色素含量改变、细胞渗透调节、离子和水稳态控制等,这些机制稳定了胞质谷胱甘肽氧化还原电位等。植物对盐的敏感程度取决于盐毒性水平、生长阶段、生理和遗传因素。为了研究耐盐品种,阐明这一复杂生物过程的机制,本研究分析了4种NaCl浓度(0-50-100-200mM)对5种面包小麦(Triticum aestivum L.)苗期生长参数(根、茎、叶长度)、色素含量(chla、chlb、类胡萝卜素)和谷胱甘肽含量的影响。基于生物特征参数,品种Nogal的色素合成和GSH含量对盐敏感(生长和色素减少);品种“Viktoria”为中等耐受性(生长部分受损,色素不变),品种“Toborzo”和“Suba”为中等耐受性(生长部分受损,色素增加),品种“Dajti”为耐盐性(生长部分受损/叶片发达,色素增加)。不同盐度对谷胱甘肽含量的响应具有品种特异性,在所有研究品种中,暴露于盐度的时间与谷胱甘肽含量呈负相关。
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引用次数: 8
Evaluation of Achillea millefolium essential oil compounds and biological effects on cervix cancer HeLa cell line 千层花精油化合物对宫颈癌症HeLa细胞系生物学效应的评价
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0003
M. B. Acar, Ebru Ibis, A. Şimşek, C. Vural, C. Tez, S. Özcan
Abstract Yarrow essential oil is used in complementary and alternative therapy for several diseases. Biological effects of essential oils span various cells and microorganisms. The aim of this study was to investigate the effects of different concentrations of the essential oil obtained from the yarrow plant (Achillea millefolium) on HeLa (CCL-2) cells. The components of the essential oil were studied by means of GC-MS analysis. Out of 10 determined compounds in the essential oil; 1,8-Cineole, Camphor, Beta-eudesmol and Camphene were found to be higher than others; and their biological effects were depicted with Ingeniuty Pathway Analysis (IPA) analysis. Moreover, cell cycle and proliferation tests were conducted on HeLa cells where yarrow plant’s essential oil was used. When extracted yarrow oil applied on HeLA Cells, apoptotic effects had been determined, furthermore proliferation of these cells decreased. In addition, activation of cell cycle control points was observed . Essential oil components could arrest the development of HeLa cells due to induction of cellular damage control mechanisms. In conclusion, we propose that the essential oil had a more repressive effect on HeLa cells, decreases their proliferation and prevented the increase in the number of cells.
摘要雅罗精油可用于多种疾病的补充和替代治疗。精油的生物学效应涉及各种细胞和微生物。本研究的目的是研究从yarrow植物(Achillea millefolium)中获得的不同浓度的精油对HeLa(CCL-2)细胞的影响。采用气相色谱-质谱联用技术对该精油的成分进行了研究。在精油中测定的10种化合物中;1,8-Cineole、Camphor、Beta eudesmol和Camphene的含量高于其他化合物;并通过Ingeniuty Pathway Analysis(IPA)分析描述了它们的生物学效应。此外,在使用了yarrow植物精油的HeLa细胞上进行了细胞周期和增殖试验。当提取的亚罗油应用于HeLA细胞时,已经确定了凋亡作用,并且这些细胞的增殖降低。此外,观察到细胞周期控制点的激活。精油成分可能由于诱导细胞损伤控制机制而阻止HeLa细胞的发育。总之,我们认为精油对HeLa细胞具有更大的抑制作用,降低了它们的增殖,并阻止了细胞数量的增加。
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引用次数: 4
Molecular docking study of L-Asparaginase I from Vibrio campbellii in the treatment of acute lymphoblastic leukemia (ALL) 坎贝尔弧菌l -天冬酰胺酶I治疗急性淋巴细胞白血病的分子对接研究
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0002
A. K. Mohideen
Abstract The potential use of asparaginases has gained tremendous significance in the treatment of acute lymphoblastic leukemia (ALL). Earlier studies suggest L-asparaginases (L-ASP) extracted from Escherichia coli and Erwinia aroideae regulates L-asparagine (L-Asn) from the circulating blood. Prolonged exposure to these enzymes may lead to hypersensitivity reactions. So, it is important to find novel asparaginases with anti-cancer properties. The three-dimensional structure of L-ASP I from Vibrio campbellii was determined by homology modeling using EasyModeller v.4.0. The structure was validated with quality indexing tools and was deposited in Protein Model DataBase. Molecular docking was performed between L-ASP I and ligand substrate L-Asn to study enzyme-substrate interactions. Qualitative and quantitative analysis of L-ASP I enzyme was found to be reliable and stable with a significant protein quality factor (LG score: 7.129). The enzyme is a dimer, belongs to α/β class of proteins. The active sites comprises of N-glycosylation site and a catalytic triad (T14-S117-D92). The binding energy of the docked complex was calculated to be -7.45 kcal/mol. The amino acid T14 identified as a primary nucleophile essential for catalytic reaction. The enzyme L-ASP I of V. campbellii provides a detailed view of structure and functional aspects with ligand substrate L-Asn. This in silico investigation has explicitly demonstrated for the first time that cytosolic L-ASP Type I of V. campbellii to have a catalytic triad which was attributed only to periplasmic L-ASP Type II. Thus, L-ASP I can serve as anti-leukemic agent in the treatment, management and control of ALL.
天冬酰胺酶在急性淋巴细胞白血病(ALL)治疗中的潜在应用具有重要意义。早期的研究表明,从大肠杆菌和避免大肠杆菌中提取的l -天冬酰胺酶(L-ASP)调节循环血液中的l -天冬酰胺(L-Asn)。长期接触这些酶可能导致过敏反应。因此,寻找具有抗癌特性的新型天冬酰胺酶具有重要意义。利用easymodeler v.4.0进行同源性建模,确定了cambelllii弧菌L-ASP I的三维结构。用质量索引工具对该结构进行了验证,并将其存储在Protein Model DataBase中。在L-ASP I和配体底物L-Asn之间进行分子对接,研究酶与底物的相互作用。L-ASP I酶的定性和定量分析可靠、稳定,具有显著的蛋白质品质因子(LG评分:7.129)。该酶为二聚体,属于α/β类蛋白。活性位点包括n -糖基化位点和催化三联体(T14-S117-D92)。计算得到配合物的结合能为-7.45 kcal/mol。氨基酸T14被确定为催化反应必需的初级亲核试剂。campbelllii的L-ASP I酶提供了与配体底物L-Asn的结构和功能方面的详细视图。本研究首次明确证明了坎贝尔弧菌胞质L-ASP I型具有催化三联体,而这种催化三联体仅归因于胞质周围的L-ASP II型。因此,L-ASP可作为抗白血病药物用于ALL的治疗、管理和控制。
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引用次数: 8
The role of C-terminal amidation in the mechanism of action of the antimicrobial peptide aurein 1.2 C-末端酰胺化在抗菌肽aurein 1.2作用机制中的作用
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0004
M. Shahmiri, A. Mechler
Abstract C-terminal amidation is a common feature of wild type membrane disrupting antimicrobial peptides (AMPs). Empirical evidence suggests that this modification increases antimicrobial efficacy. However, the actual role of C-terminal amidation in the molecular mechanism of action of AMPs is not fully understood. Amidation alters two key properties simultaneously: the net charge and helicity of the peptide, both of which are implicated in the mechanism of action. However, the differences between the physicochemical properties of the carboxyl and amide moieties have been disregarded in former studies. In this study we assessed whether the difference in activity is only caused by changes in the helicity and overall charge of a peptide, i.e. whether the chemistry of the terminus is otherwise irrelevant. To do so, the membrane disrupting activity of a modified aurein 1.2 peptide was studied in which a secondary amide was formed with a terminal methyl group, instead of the primary amide as in the wild type peptide. Results of quartz crystal microbalance, dye leakage and circular dichroism experiments show that the activity of the modified peptide is substantially reduced compared to the wild type peptide, in particular that the modified peptide exhibited a much-reduced ability to bind to the membrane. Thus, the primary amide at the C-terminus is required to bind to the membrane, and a secondary amide cannot serve the same purpose. We hypothesize that this difference is related to the hydration state of the terminus. The lack of membrane binding ability of the modified peptide identifies the primary amide moiety at the C terminus as a specific membrane binding motif.
摘要C-末端酰胺化是野生型破膜抗菌肽(AMP)的一个常见特征。经验证据表明,这种修饰提高了抗菌效果。然而,C末端酰胺化在AMPs作用的分子机制中的实际作用尚不完全清楚。酰胺化同时改变了两个关键特性:肽的净电荷和螺旋度,这两个特性都与作用机制有关。然而,在以前的研究中,羧基和酰胺部分的物理化学性质之间的差异被忽略了。在这项研究中,我们评估了活性的差异是否只是由肽的螺旋度和总电荷的变化引起的,即末端的化学性质是否在其他方面无关。为此,研究了修饰的aurein 1.2肽的膜破坏活性,其中用末端甲基形成仲酰胺,而不是野生型肽中的伯酰胺。石英晶体微量天平、染料渗漏和圆二色性实验的结果表明,与野生型肽相比,修饰肽的活性显著降低,特别是修饰肽与膜结合的能力大大降低。因此,C末端的伯酰胺需要与膜结合,而仲酰胺不能起到相同的作用。我们假设这种差异与末端的水合状态有关。修饰肽的膜结合能力的缺乏将C末端的伯酰胺部分鉴定为特定的膜结合基序。
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引用次数: 10
Effects of zinc and molybdenum on European Bluestar (Amsonia orientalis): An in vitro study 锌和钼对欧洲蓝星(Amsonia orientalis)影响的体外研究
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-01-01 DOI: 10.2478/ebtj-2020-0005
Arda Acemi, Y. Duman, Y. Y. Karakus, Fazıl Özen
Abstract This study aimed to investigate the effects of possible zinc (Zn) and molybdenum (Mo) contaminations on the critically endangered European Bluestar (Amsonia orientalis). The effects of Zn and Mo were tested in a dose-dependent manner on in vitro cultures. Zn at 0.1 mM in the medium inhibited root development whereas Mo showed the same effect only at ≥2.5 mM concentration. Gradual inhibition of shoot development was observed after treatment with both metals. Protein contents were also negatively affected by increasing metal concentrations, while proline levels increased gradually. Successive increases in metal concentrations resulted in higher hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations. The activity of the antioxidant enzymes, peroxidase (POD) and catalase (CAT), were found to be enhanced in response to increasing metal concentrations. Superoxide dismutase (SOD) activity decreased after Zn treatment but increased after Mo treatment. A marked increase in POD and CAT in response to metal stress suggests that these enzymes might have a significant cooperative role in regulating H2O2 production, although CAT, in response to drought and salt stress, has been reported to only play a supplementary role in A. orientalis. These results indicated that A. orientalis is susceptible to long-term Zn stress but can tolerate up to 2.5 mM Mo in the long-term. Deficiency of Mo is more common than high toxic concentrations in the environment. Therefore Zn contamination should be considered as one of the major threats for A. orientalis in its native habitat.
摘要本研究旨在探讨可能存在的锌(Zn)和钼(Mo)污染对濒危欧洲蓝星(Amsonia orientalis)的影响。锌和钼对体外培养的影响呈剂量依赖性。培养基中0.1 mM的Zn对根系发育有抑制作用,而浓度≥2.5 mM的Mo对根系发育有抑制作用。两种金属处理后,茎的发育逐渐受到抑制。金属浓度的增加对蛋白质含量也有负面影响,而脯氨酸含量则逐渐增加。金属浓度的连续增加导致过氧化氢(H2O2)和丙二醛(MDA)浓度升高。抗氧化酶过氧化物酶(POD)和过氧化氢酶(CAT)的活性随着金属浓度的增加而增强。超氧化物歧化酶(SOD)活性在Zn处理后降低,Mo处理后升高。金属胁迫下POD和CAT的显著增加表明,这两种酶可能在调控H2O2生产中起着重要的协同作用,尽管CAT在干旱和盐胁迫下仅起辅助作用。结果表明,东方蒿对长期Zn胁迫敏感,但能长期耐受高达2.5 mM的Zn胁迫。钼缺乏比环境中有毒的高浓度更为常见。因此,锌污染应被认为是东方桦原生生境的主要威胁之一。
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引用次数: 5
Differences in organic matter quality, chemical and microbiological characteristics of two Phaeozems under natural and anthropic influence 自然和人为影响下两种Phaeozems有机质质量、化学和微生物特性的差异
IF 1.7 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2019-10-01 DOI: 10.2478/ebtj-2019-0022
M. Sorin, G. Matei, Dumitrașcu Monica, V. Mocanu
Abstract The soil degraded and changed by the anthropic activity must be monitored and the awareness of the intrinsic processes will allow a pertinent analysis of the effects of the application of the new technologies on the management and the sustainability of the soil. Two natural and anthropic Phaeozems were analyzed from the point of view of chemical, microbiological characteristics, quality and composition of organic compounds. Total values of microbial biomass and bacterial and fungal counts were generally twice higher in Calcaric Phaeozems than in Verti-Stagnic Phaeozems. The content of humic precursors in Calcaric Phaeozems was quantitatively higher than that determined in Verti-stagnic Phaeozems, with a total content of phenols of 14.6mgGAExg−1d.m., polysaccharides and proteins of 97mgxg−1, respectivelly 16.6mgxl−1. The ascending chromatograms showed specific distribution and higher density of the organic compounds in the CAFT sub-fraction of the Verti-stagnic Phaeozems. Pfeiffer specific chromatograms revealed an enzyme activity much higher than average at the Verti-stagnic Phaeozems, with a well-characterized functional diversity. The nutritional reserve appeared increased but poorly diversified in the Calcaric Phaeozems. Humification processes are intense, colloidal substances are present, the mineral component is very well integrated in the organic material at the Verti-stagnic Phaeozems and complex protein content is well revealed especially in the Calcaric Phaeozems. Capillary dynamolysis reflected a characteristic pattern of Phaeozems soils, with particularities for each soil type, represented by colors, contours and particular forms of the specific structures developed. Both soils presented good conditions for sustaining vegetation either natural or cultivated but results indicated that anthropic intervention determined a more dynamic mineralization of organic matter. Further monitoring of soil organic matter dynamics is needed and adjusting management practices for conservation of biodiversity and global ecosystem protection against the effect of anthropic intervention.
人类活动对土壤的退化和变化必须进行监测,认识其内在过程将有助于对新技术应用对土壤管理和可持续性的影响进行有针对性的分析。从化学、微生物特性、质量和有机物组成等方面分析了两种天然的和人为的Phaeozems。石灰腐菌的微生物总量、细菌和真菌数量一般比灰腐菌高2倍。钙化腐殖菌中腐殖质前体的含量高于垂直静止腐殖菌,总酚含量为14.6mgGAExg−1d.m。,多糖97mgxl−1,蛋白质16.6mgxl−1。从上升层析图可以看出,垂直静止Phaeozems的CAFT亚组分中有机化合物的密度较高,分布特殊。特异色谱显示,垂直静止Phaeozems的酶活性远高于平均水平,具有良好的功能多样性特征。钙质phaeozem的营养储备有所增加,但多样性较差。腐殖化作用强烈,存在胶体物质,垂直静止phaezem的矿物成分在有机物质中整合得很好,特别是在钙质phaezem中显示出复杂的蛋白质含量。毛细动力分解反应了Phaeozems土壤的特征模式,每种土壤类型都具有特殊性,由颜色、轮廓和特定结构的特定形式所代表。两种土壤都具有良好的维持植被的条件,但结果表明,人为干预决定了有机质的动态矿化。需要进一步监测土壤有机质动态,调整管理措施,以保护生物多样性和全球生态系统免受人为干预的影响。
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引用次数: 1
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The EuroBiotech Journal
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