Y. Kang, Deuk‐Su Kim, Kibum Kim, S. Myung, Y. J. Oh, Sungsu Park, P. Hinterdorfer, K. Ko
Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.
{"title":"Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant","authors":"Y. Kang, Deuk‐Su Kim, Kibum Kim, S. Myung, Y. J. Oh, Sungsu Park, P. Hinterdorfer, K. Ko","doi":"10.2478/ebtj-2023-0006","DOIUrl":"https://doi.org/10.2478/ebtj-2023-0006","url":null,"abstract":"Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49542025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ciabattini, V. Raggi, L. Valentini, A. Morabito
Abstract Background Regenerative Medicine (RM) is a branch of medicine that aims to regenerate tissues and organs to overcome the problems transplants entail (poor availability, risk of rejection and intense immunosuppression). To do this, RM makes use of tissue engineering (TE). This fundamental branch deals with creating biological scaffolds capable of performing the role that physiologically belongs to the extracellular matrix (ECM). In this review, we report how specific characteristics of the scaffolds (bio-compatibility, biodegradability and mechanical and conformal properties) can be obtained using 3D printing, which facilitates the emulation of physiological tissues and organs. Purpose and scope This review reports recent advances in the fabrication method of bioactive scaffolds that can be used clinically, providing support for cell seeding and proliferation. To this end, silk fibroin, tannin and graphene were used to improve the scaffold’s electro-bio-mechanical properties. These materials in different compositions are studied to demonstrate their potential use as bio-ink in bioadhesives and cellularized and implantable 3D-printed scaffolds. Summary of new synthesis and conclusions reached in the review Silk fibroin is a natural biopolymer; tannin, on the other hand, is a biological polyphenol, highly reactive with other molecules by nature and with promising antioxidant capabilities. Finally, graphene is nothing more than a monolayer of graphite that has been shown to implement the mechanics and electrical conductivity of the compounds in which it is inserted; it also has excellent biocompatibility and surface area, qualities that promote cell adhesion and growth. Conclusion Polyphenols and graphene have been shown to work in synergy in improving the electro-mechanical properties of silk fibroin scaffolds. We reported optimal and potentially market-competitive bioadhesives, but above all, the proliferation of neuronal precursor cells in vitro was successfully demonstrated.
{"title":"Silk Fibroin Hybrids for Biological Scaffolds with Adhesive Surface and Adaptability to the Target Tissue Change","authors":"S. Ciabattini, V. Raggi, L. Valentini, A. Morabito","doi":"10.2478/ebtj-2023-0005","DOIUrl":"https://doi.org/10.2478/ebtj-2023-0005","url":null,"abstract":"Abstract Background Regenerative Medicine (RM) is a branch of medicine that aims to regenerate tissues and organs to overcome the problems transplants entail (poor availability, risk of rejection and intense immunosuppression). To do this, RM makes use of tissue engineering (TE). This fundamental branch deals with creating biological scaffolds capable of performing the role that physiologically belongs to the extracellular matrix (ECM). In this review, we report how specific characteristics of the scaffolds (bio-compatibility, biodegradability and mechanical and conformal properties) can be obtained using 3D printing, which facilitates the emulation of physiological tissues and organs. Purpose and scope This review reports recent advances in the fabrication method of bioactive scaffolds that can be used clinically, providing support for cell seeding and proliferation. To this end, silk fibroin, tannin and graphene were used to improve the scaffold’s electro-bio-mechanical properties. These materials in different compositions are studied to demonstrate their potential use as bio-ink in bioadhesives and cellularized and implantable 3D-printed scaffolds. Summary of new synthesis and conclusions reached in the review Silk fibroin is a natural biopolymer; tannin, on the other hand, is a biological polyphenol, highly reactive with other molecules by nature and with promising antioxidant capabilities. Finally, graphene is nothing more than a monolayer of graphite that has been shown to implement the mechanics and electrical conductivity of the compounds in which it is inserted; it also has excellent biocompatibility and surface area, qualities that promote cell adhesion and growth. Conclusion Polyphenols and graphene have been shown to work in synergy in improving the electro-mechanical properties of silk fibroin scaffolds. We reported optimal and potentially market-competitive bioadhesives, but above all, the proliferation of neuronal precursor cells in vitro was successfully demonstrated.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48612361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Javier S Martinez de Aragon, J. Villada, J. Ruiz-Moreno
Abstract Introduction: The biocompatibility of nitinol in the human body has extensively been demonstrated. Although nitinol is already being used for intraocular surgeries such as lens fragmentation and foreign body extraction, little is known about its intracapsular, long-term behavior. The purpose of this study is to evaluate the long-term uveal and capsular biocompatibility of a nitinol CTR placed in the capsular bag after cataract surgery in an animal model. Method: After approval of the study by the Institutional Animal Care and the Ethics Committee, bilateral phacoemulsification was performed in 6 rabbits; 1 eye received a nitinol CTR and the other a control polymethylmethacrylate (PMMA) open-ended ring. Ophthalmic evaluation for the presence of infections in all 12 eyes was performed after 7 days, 4 weeks, 3 months, and 6 months follow-up period. After a follow-up period of 6 months, the eyes were enucleated, and a histopathologic evaluation was performed. Results: Neither of the groups showed any clinical signs of posterior capsule opacification (PCO) or inflammation. The nitinol group showed slightly less inflammation during histopathologic examination compared to the PMMA group. No biocompatibility issues have been observed in this animal study. Conclusions: There were no histological differences between eyes implanted with nitinol and eyes implanted with PMMA rings. Nitinol has proven to show high biocompatibility when implanted in the capsular bag of the rabbit eye.
{"title":"Ocular Biocompatibility of a Nitinol Capsular Tension Ring (CTR)","authors":"Javier S Martinez de Aragon, J. Villada, J. Ruiz-Moreno","doi":"10.2478/ebtj-2022-0016","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0016","url":null,"abstract":"Abstract Introduction: The biocompatibility of nitinol in the human body has extensively been demonstrated. Although nitinol is already being used for intraocular surgeries such as lens fragmentation and foreign body extraction, little is known about its intracapsular, long-term behavior. The purpose of this study is to evaluate the long-term uveal and capsular biocompatibility of a nitinol CTR placed in the capsular bag after cataract surgery in an animal model. Method: After approval of the study by the Institutional Animal Care and the Ethics Committee, bilateral phacoemulsification was performed in 6 rabbits; 1 eye received a nitinol CTR and the other a control polymethylmethacrylate (PMMA) open-ended ring. Ophthalmic evaluation for the presence of infections in all 12 eyes was performed after 7 days, 4 weeks, 3 months, and 6 months follow-up period. After a follow-up period of 6 months, the eyes were enucleated, and a histopathologic evaluation was performed. Results: Neither of the groups showed any clinical signs of posterior capsule opacification (PCO) or inflammation. The nitinol group showed slightly less inflammation during histopathologic examination compared to the PMMA group. No biocompatibility issues have been observed in this animal study. Conclusions: There were no histological differences between eyes implanted with nitinol and eyes implanted with PMMA rings. Nitinol has proven to show high biocompatibility when implanted in the capsular bag of the rabbit eye.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42290663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Background: Climate change affects every region across the globe with heterogeneous effects on local temperatures and precipitation patterns. In plants, sessile organisms, climate change imposes more drastic effects leading to loss of yield or even death. However, plants establish mutualistic interactions with microorganisms that boost plant tolerance against abiotic stresses or strengthen the plant immune system against pathogens, thus, enhancing their survival and fitness. Moreover, in the wild, microbial endophytes provide important ecosystem services. Purpose and scope: Little we know about the mechanisms of response against the adverse effects of climate change on natural populations of wild plants and even less about the potential role played by microbial biostimulants. In this article, we review the effects of biostimulants on plant responses against abiotic stresses, with a particular focus on the role of mycorrhizas and leaf endophytes. Results: We have reviewed the effects of the main abiotic stresses in plants, the mechanisms that plants use to face these abiotic challenges, and the interaction plant-biostimulant-abiotic stress, highlighting the primary responses and parameters to evaluate different plant responses. Conclusion: Abiotic stresses can check the phenotypic plasticity of plants and also trigger a complex and heterogeneous array of responses to face different abiotic stresses, and beneficial microorganisms do play an essential role in enhancing such responses. Our laboratory has initiated a project to characterise microbial populations associated with plants from wild areas and analyse their potential role in aiding the plants to cope with abiotic stresses.
{"title":"The Microbially Extended Phenotype of Plants, a Keystone against Abiotic Stress","authors":"Mario X. Ruiz-González, Ó. Vicente","doi":"10.2478/ebtj-2022-0017","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0017","url":null,"abstract":"Abstract Background: Climate change affects every region across the globe with heterogeneous effects on local temperatures and precipitation patterns. In plants, sessile organisms, climate change imposes more drastic effects leading to loss of yield or even death. However, plants establish mutualistic interactions with microorganisms that boost plant tolerance against abiotic stresses or strengthen the plant immune system against pathogens, thus, enhancing their survival and fitness. Moreover, in the wild, microbial endophytes provide important ecosystem services. Purpose and scope: Little we know about the mechanisms of response against the adverse effects of climate change on natural populations of wild plants and even less about the potential role played by microbial biostimulants. In this article, we review the effects of biostimulants on plant responses against abiotic stresses, with a particular focus on the role of mycorrhizas and leaf endophytes. Results: We have reviewed the effects of the main abiotic stresses in plants, the mechanisms that plants use to face these abiotic challenges, and the interaction plant-biostimulant-abiotic stress, highlighting the primary responses and parameters to evaluate different plant responses. Conclusion: Abiotic stresses can check the phenotypic plasticity of plants and also trigger a complex and heterogeneous array of responses to face different abiotic stresses, and beneficial microorganisms do play an essential role in enhancing such responses. Our laboratory has initiated a project to characterise microbial populations associated with plants from wild areas and analyse their potential role in aiding the plants to cope with abiotic stresses.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45959030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Background: Prior to 1970, women authored or co-authored a variety of eponymously named psychometric tests predominately in children and young adults with developmental and behavioral disorders. Gaps remain in understanding and appreciating the extent of their contributions to these psychometric tests. Methods: Two authors adjudicated psychometric tests in the text, Tests in Print, to identify those eponymously named after women. To be eligible for this study, the psychometric test title must contain the name of a woman and be published prior to 1970. If a woman published more than one eponymously named test, the earliest one, if available, was chosen to be included in this study. A search for individual and a combination of related Medical Subject Headings (MeSH) keywords of the “name of the women author” and “name of the test” was performed using the Google web browser, PubMed, APA PsycNet, and APA PsycInfo from inception to August 1, 2021. Further papers were identified using bibliographic search from source papers and reference books. Results: Three hundred eighty-six eponymously named psychometric tests were identified, with 124 (32%) of them named in whole or in part by or for a woman. Of these 124 psychometric tests, 93 represent unique women’s names. Before 1970, there were twenty-three (19%) eponymously named psychometric tests with women, with twenty-one (17%) representing unique women’s names. Conclusion: These tests were published by women whereby they served either as a single author or in collaboration with others or their spouse. In the latter case, the woman’s name universally appeared after their husband’s name. In only a few cases, are these women’s names well-referenced in biographical source materials. These women made essential contributions to the development and revision of psychometric tests and the field of psychology in general.
{"title":"Historical Aspects and Test Characteristics of Eponymously Named Psychometric Tests by Women: The Period up to 1970","authors":"Allison Yale, A. Colaiezzi, H. Tekiner, S. Yale","doi":"10.2478/ebtj-2022-0015","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0015","url":null,"abstract":"Abstract Background: Prior to 1970, women authored or co-authored a variety of eponymously named psychometric tests predominately in children and young adults with developmental and behavioral disorders. Gaps remain in understanding and appreciating the extent of their contributions to these psychometric tests. Methods: Two authors adjudicated psychometric tests in the text, Tests in Print, to identify those eponymously named after women. To be eligible for this study, the psychometric test title must contain the name of a woman and be published prior to 1970. If a woman published more than one eponymously named test, the earliest one, if available, was chosen to be included in this study. A search for individual and a combination of related Medical Subject Headings (MeSH) keywords of the “name of the women author” and “name of the test” was performed using the Google web browser, PubMed, APA PsycNet, and APA PsycInfo from inception to August 1, 2021. Further papers were identified using bibliographic search from source papers and reference books. Results: Three hundred eighty-six eponymously named psychometric tests were identified, with 124 (32%) of them named in whole or in part by or for a woman. Of these 124 psychometric tests, 93 represent unique women’s names. Before 1970, there were twenty-three (19%) eponymously named psychometric tests with women, with twenty-one (17%) representing unique women’s names. Conclusion: These tests were published by women whereby they served either as a single author or in collaboration with others or their spouse. In the latter case, the woman’s name universally appeared after their husband’s name. In only a few cases, are these women’s names well-referenced in biographical source materials. These women made essential contributions to the development and revision of psychometric tests and the field of psychology in general.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42439771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ö. Işeri, D. A. Körpe, F. Sahin, E. Cabi, M. Haberal
Abstract Aim of this study was to comparatively evaluate antibacterial activities of methanol (MetOH), acetone (Ace), petroleum ether (PE) and aqueous (dw) leaf (L), root (R), and seed (S) extracts of Corchorus olitorius L. on both food- and plant-borne pathogens, with DPPH radical scavenging activities (DRSA), and quantitative and qualitative constituent analysis. Leaf PE has the highest strain susceptibility on both food- and plant-borne pathogens. Clavibacter michiganensis, Pseudomonas tomato, and Erwinia caratovora were susceptible to nearly all the leaf and seed extracts. Very low minimum inhibitory concentration (8-128 μg mL−1) and minimum bactericidal concentration (32-2048 μg mL−1) were determined for both leaf and seed extracts against C. michiganensis. Total phenolic contents were correlated to DRSA. The phenolic compounds tested were higher in the leaf MetOH, cholorogenic acid being the most abundant one. Palmitic acid was determined in leaf PE and seed PE extracts. Results presented here demonstrate high antibacterial activity of C. olitorius leaf seed extracts against phytopathogens for the first time, and provide the most comprehensive data on the antibacterial activity screening against food-borne pathogens. Considering limitations in plant disease control, antibacterial activities of these extracts would be important in plant disease control.
{"title":"Corchorus olitorius L. (Jute) leaf and seed extracts exerted high antibacterial activity against food and plant pathogenic bacteria","authors":"Ö. Işeri, D. A. Körpe, F. Sahin, E. Cabi, M. Haberal","doi":"10.2478/ebtj-2022-0011","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0011","url":null,"abstract":"Abstract Aim of this study was to comparatively evaluate antibacterial activities of methanol (MetOH), acetone (Ace), petroleum ether (PE) and aqueous (dw) leaf (L), root (R), and seed (S) extracts of Corchorus olitorius L. on both food- and plant-borne pathogens, with DPPH radical scavenging activities (DRSA), and quantitative and qualitative constituent analysis. Leaf PE has the highest strain susceptibility on both food- and plant-borne pathogens. Clavibacter michiganensis, Pseudomonas tomato, and Erwinia caratovora were susceptible to nearly all the leaf and seed extracts. Very low minimum inhibitory concentration (8-128 μg mL−1) and minimum bactericidal concentration (32-2048 μg mL−1) were determined for both leaf and seed extracts against C. michiganensis. Total phenolic contents were correlated to DRSA. The phenolic compounds tested were higher in the leaf MetOH, cholorogenic acid being the most abundant one. Palmitic acid was determined in leaf PE and seed PE extracts. Results presented here demonstrate high antibacterial activity of C. olitorius leaf seed extracts against phytopathogens for the first time, and provide the most comprehensive data on the antibacterial activity screening against food-borne pathogens. Considering limitations in plant disease control, antibacterial activities of these extracts would be important in plant disease control.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46940752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The term “vital sign” has been assigned to various phenomena with the presumptive intent to emphasize their importance in health care resulting in the emergence of eight vital signs with multiple designations and overlapping terms. This review developed a case definition for vital signs and identified and described the fifth through eighth vital signs. PubMed/Medline, Google and biographical databases were searched using the individual Medical Subject Headings (MeSH) terms, vital sign and fifth, vital sign and sixth, vital sign and seventh, and vital sign eighth. The search was limited to human clinical studies written in English literature from 1957 up until November 30, 2021. Excluded were articles containing the term vital sign if used alone without the qualifier fifth, sixth, seventh, or eighth or about temperature, blood pressure, pulse, and respiratory rate. One hundred ninety-six articles (122 for the fifth vital sign, 71 for the sixth vital sign, two for the seventh vital sign, and one for the eighth vital sign) constituted the final dataset. The vital signs consisted of 35 terms, classified into 17 categories compromising 186 unique papers for each primary authored article with redundant numbered vital signs for glucose, weight, body mass index, and medication compliance. Eleven terms have been named the fifth vital sign, 25 the sixth vital sign, three the seventh, and one as the eighth vital sign. There are four time-honored vital signs based on the case definition, and they represent an objective bedside measurement obtained noninvasively that is essential for life. Based on this case definition, pulse oximetry qualifies as the fifth while end-tidal CO2 and cardiac output as the sixth. Thus, these terms have been misappropriated 31 times. Although important to emphasize in patient care, the remainder are not vital signs and should not be construed in this manner.
{"title":"A Comprehensive Assessment of The Eight Vital Signs","authors":"J. Draves, H. Tekiner, ES Yale, JJ Mazza, SH Yale","doi":"10.2478/ebtj-2022-0014","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0014","url":null,"abstract":"Abstract The term “vital sign” has been assigned to various phenomena with the presumptive intent to emphasize their importance in health care resulting in the emergence of eight vital signs with multiple designations and overlapping terms. This review developed a case definition for vital signs and identified and described the fifth through eighth vital signs. PubMed/Medline, Google and biographical databases were searched using the individual Medical Subject Headings (MeSH) terms, vital sign and fifth, vital sign and sixth, vital sign and seventh, and vital sign eighth. The search was limited to human clinical studies written in English literature from 1957 up until November 30, 2021. Excluded were articles containing the term vital sign if used alone without the qualifier fifth, sixth, seventh, or eighth or about temperature, blood pressure, pulse, and respiratory rate. One hundred ninety-six articles (122 for the fifth vital sign, 71 for the sixth vital sign, two for the seventh vital sign, and one for the eighth vital sign) constituted the final dataset. The vital signs consisted of 35 terms, classified into 17 categories compromising 186 unique papers for each primary authored article with redundant numbered vital signs for glucose, weight, body mass index, and medication compliance. Eleven terms have been named the fifth vital sign, 25 the sixth vital sign, three the seventh, and one as the eighth vital sign. There are four time-honored vital signs based on the case definition, and they represent an objective bedside measurement obtained noninvasively that is essential for life. Based on this case definition, pulse oximetry qualifies as the fifth while end-tidal CO2 and cardiac output as the sixth. Thus, these terms have been misappropriated 31 times. Although important to emphasize in patient care, the remainder are not vital signs and should not be construed in this manner.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45597009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Eraslan, Yavuz Erden, S. Oruç, B. Bircan, Sevilay Gunay
Abstract There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines. After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.
{"title":"Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage","authors":"E. Eraslan, Yavuz Erden, S. Oruç, B. Bircan, Sevilay Gunay","doi":"10.2478/ebtj-2022-0010","DOIUrl":"https://doi.org/10.2478/ebtj-2022-0010","url":null,"abstract":"Abstract There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines. After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47151417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：